MD13G1156300.v1.1

Zinc finger CCHC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007741
Physical Location & Seq
Reverse (-)
12180240 .. 12182552
2313 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1156300.v1.1.491

Sequence Viewer

Length: 786 bp
ATGTCAAGTAAGAAAGAAGAGAAAGCTCAAGCTGCGGCTGAAAGGATCAAGGCAGCAGCCTTGAGTGCTGCAAAAGGTCTTAGTCGAGCTCAGGCTGAAAGGGCAGCTGCTGCAGCTGCTCGGAATGTTAATGCTTATGGGCAGAAGGAAGAAGGACCCAGCAGATGGCAGGAGAAAAGGGAAGCCAAGAGACAGATGTATTTGATGAGTACTGAAAAGGCTGTGAGATTGGGTGAACGGAAAGACCTCAAGTCAAATGTGTCTACCGTTGGTGGTGCAGCTTCACAATGCCAGAAGTGTTACCAACCCGGGCATTGGACATATGAATGCAAGAATGAACGCGTTTACATCTCGAGACCGTCTAGGACCCAGCAACTCAAAAATCCTAAACTGAAGATGAAGGTTGAAATATCATATGATTTGGACAGTCCAGAAATTAAGGAAGAGAAGGAAGAAAAGCAGTCAAAGAAAAGCAAGAGGAAGCATCGCTCAGCCTCTGATTCCGGAAGTGATAGCGAGGCTTCAGTTTTTGAGACTGATAGTGGTGCATCATCAGTTACAGGATCTGAATATTCTTCGGAGGAGAGTAGCTCAGATTACAGCTCATCTACTGACTCAGAGGAGGAGAGGAGGCACCGAAGGAAGAAGAAGAAGCAAAAGATGGCAAGGCGTAGAAGAAGGTACAGCTCATCATCTGAATCAACTGATTCTGAGTCAGCTTCCGAATCTGATTCTGATGATAGGAGGAGCCGGAAGAAGAGCACGAGGCACGACAGAAAGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC_3

Protein Analysis

262

Amino Acids

29.52

Weight (kDa)

9.73

Isoelectric Point (pI)

86.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_3 PF13917 93 - 129 1.2e-21 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 633
AccB7I CCANNNNNTGG 1 cut(s) 165
AccI GTMKAC 1 cut(s) 263
AccII CGCG 1 cut(s) 342
AccIII TCCGGA 1 cut(s) 503
AciI CCGC 1 cut(s) 35
AclWI GGATC 2 cut(s) 53, 571
AcuI CTGAAG 2 cut(s) 413, 507
AfaI GTAC 2 cut(s) 211, 683
AfiI CCNNNNNNNGG 2 cut(s) 165, 315
AflIII ACRYGT 1 cut(s) 340
AgsI TTSAA 1 cut(s) 407
AhdI GACNNNNNGTC 1 cut(s) 250
Alw21I GWGCWC 2 cut(s) 91, 764
Alw26I GTCTC 3 cut(s) 184, 349, 527
AlwI GGATC 2 cut(s) 53, 571
AlwNI CAGNNNCTG 3 cut(s) 110, 497, 566
Ama87I CYCGRG 2 cut(s) 308, 352
Aor13HI TCCGGA 1 cut(s) 503
AspS9I GGNCC 2 cut(s) 155, 366
AsuC2I CCSGG 2 cut(s) 309, 310
AsuHPI GGTGA 1 cut(s) 245
AvaI CYCGRG 2 cut(s) 308, 352
AvaII GGWCC 2 cut(s) 155, 366
BanI GGYRCC 1 cut(s) 633
BanII GRGCYC 1 cut(s) 91
BauI CACGAG 1 cut(s) 763
Bbv12I GWGCWC 2 cut(s) 91, 764
BccI CCATC 2 cut(s) 159, 655
BcnI CCSGG 2 cut(s) 309, 310
BcoDI GTCTC 3 cut(s) 184, 349, 527
BfaI CTAG 1 cut(s) 363
BfmI CTRYAG 1 cut(s) 111
BlpI GCTNAGC 1 cut(s) 490
BmcAI AGTACT 1 cut(s) 211
Bme1390I CCNGG 2 cut(s) 309, 310
Bme18I GGWCC 2 cut(s) 155, 366
BmeRI GACNNNNNGTC 1 cut(s) 250
BmeT110I CYCGRG 2 cut(s) 308, 352
BmgT120I GGNCC 2 cut(s) 155, 366
BmiI GGNNCC 4 cut(s) 157, 368, 635, 749
BmrFI CCNGG 2 cut(s) 309, 310
BmsI GCATC 2 cut(s) 493, 557
BplI GAGNNNNNCTC 2 cut(s) 575, 607
Bpu10I CCTNAGC 1 cut(s) 90
Bpu1102I GCTNAGC 1 cut(s) 490
BpuEI CTTGAG 3 cut(s) 12, 82, 233
BpuMI CCSGG 2 cut(s) 309, 310
BsaI GGTCTC 1 cut(s) 349
BsaJI CCNNGG 1 cut(s) 308
BsaWI WCCGGW 1 cut(s) 503
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 315
BseAI TCCGGA 1 cut(s) 503
BseDI CCNNGG 1 cut(s) 308
BseLI CCNNNNNNNGG 2 cut(s) 165, 315
BseMII CTCAG 5 cut(s) 104, 504, 606, 630, 702
BseRI GAGGAG 5 cut(s) 596, 635, 638, 643, 760
BseYI CCCAGC 2 cut(s) 158, 369
BsgI GTGCAG 1 cut(s) 297
Bsh1236I CGCG 1 cut(s) 342
BshNI GGYRCC 1 cut(s) 633
BsiHKAI GWGCWC 2 cut(s) 91, 764
BsiHKCI CYCGRG 2 cut(s) 308, 352
BsiSI CCGG 3 cut(s) 309, 504, 751
BslI CCNNNNNNNGG 2 cut(s) 165, 315
BsmAI GTCTC 3 cut(s) 184, 349, 527
BsmI GAATGC 1 cut(s) 332
Bso31I GGTCTC 1 cut(s) 349
BsoBI CYCGRG 2 cut(s) 308, 352
Bsp1286I GDGCHC 2 cut(s) 91, 764
Bsp13I TCCGGA 1 cut(s) 503
Bsp143I GATC 2 cut(s) 45, 563
Bsp1720I GCTNAGC 1 cut(s) 490
BspACI CCGC 1 cut(s) 35
BspCNI CTCAG 5 cut(s) 103, 503, 605, 629, 703
BspEI TCCGGA 1 cut(s) 503
BspFNI CGCG 1 cut(s) 342
BspLI GGNNCC 4 cut(s) 157, 368, 635, 749
BspMAI CTGCAG 1 cut(s) 115
BspPI GGATC 2 cut(s) 53, 571
BspQI GCTCTTC 1 cut(s) 752
BspT107I GGYRCC 1 cut(s) 633
BspTNI GGTCTC 1 cut(s) 349
BssECI CCNNGG 1 cut(s) 308
BssMI GATC 2 cut(s) 45, 563
BssSI CACGAG 1 cut(s) 763
Bst2BI CACGAG 1 cut(s) 763
Bst4CI ACNGT 3 cut(s) 268, 360, 428
Bst6I CTCTTC 3 cut(s) 12, 438, 752
BstAPI GCANNNNNTGC 1 cut(s) 110
BstDEI CTNAG 6 cut(s) 80, 90, 490, 592, 616, 711
BstFNI CGCG 1 cut(s) 342
BstKTI GATC 2 cut(s) 48, 566
BstMAI GTCTC 3 cut(s) 184, 349, 527
BstMBI GATC 2 cut(s) 45, 563
BstMWI GCNNNNNNNGC 6 cut(s) 32, 65, 101, 110, 113, 116
BstSCI CCNGG 2 cut(s) 307, 308
BstSFI CTRYAG 1 cut(s) 111
BstUI CGCG 1 cut(s) 342
BstX2I RGATCY 1 cut(s) 563
BstYI RGATCY 1 cut(s) 563
BtgZI GCGATG 1 cut(s) 470
CaiI CAGNNNCTG 3 cut(s) 110, 497, 566
Cfr13I GGNCC 2 cut(s) 155, 366
Cfr9I CCCGGG 1 cut(s) 308
Csp6I GTAC 2 cut(s) 210, 682
CviQI GTAC 2 cut(s) 210, 682
DdeI CTNAG 6 cut(s) 80, 90, 490, 592, 616, 711
DpnI GATC 2 cut(s) 47, 565
DpnII GATC 2 cut(s) 45, 563
DriI GACNNNNNGTC 1 cut(s) 250
Eam1104I CTCTTC 3 cut(s) 12, 438, 752
Eam1105I GACNNNNNGTC 1 cut(s) 250
EarI CTCTTC 3 cut(s) 12, 438, 752
Ecl136II GAGCTC 1 cut(s) 89
Eco24I GRGCYC 1 cut(s) 91
Eco31I GGTCTC 1 cut(s) 349
Eco47I GGWCC 2 cut(s) 155, 366
Eco53kI GAGCTC 1 cut(s) 89
Eco57I CTGAAG 2 cut(s) 413, 507
Eco88I CYCGRG 2 cut(s) 308, 352
EcoICRI GAGCTC 1 cut(s) 89
EcoO109I RGGNCCY 2 cut(s) 155, 366
EcoT38I GRGCYC 1 cut(s) 91
FaiI YATR 5 cut(s) 138, 322, 324, 415, 417
FauNDI CATATG 2 cut(s) 322, 415
FblI GTMKAC 1 cut(s) 263
FriOI GRGCYC 1 cut(s) 91
FspBI CTAG 1 cut(s) 363
GsaI CCCAGC 2 cut(s) 162, 373
HapII CCGG 3 cut(s) 309, 504, 751
HinfI GANTC 7 cut(s) 500, 614, 698, 707, 713, 725, 731
HpaII CCGG 3 cut(s) 309, 504, 751
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 3 cut(s) 236, 264, 346
Hpy188III TCNNGA 4 cut(s) 352, 354, 431, 504
Hpy8I GTNNAC 3 cut(s) 236, 264, 346
HpyAV CCTTC 6 cut(s) 139, 146, 394, 442, 633, 672
HpyCH4III ACNGT 3 cut(s) 268, 360, 428
HpyCH4V TGCA 5 cut(s) 71, 113, 278, 330, 548
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 65, 101, 110, 113, 116
HpyF3I CTNAG 6 cut(s) 80, 90, 490, 592, 616, 711
Kpn2I TCCGGA 1 cut(s) 503
Kzo9I GATC 2 cut(s) 45, 563
LguI GCTCTTC 1 cut(s) 752
LmnI GCTCC 1 cut(s) 747
LweI GCATC 2 cut(s) 493, 557
MaeI CTAG 1 cut(s) 363
MaeIII GTNAC 2 cut(s) 299, 556
MalI GATC 2 cut(s) 47, 565
MboI GATC 2 cut(s) 45, 563
MflI RGATCY 1 cut(s) 563
MhlI GDGCHC 2 cut(s) 91, 764
MluCI AATT 1 cut(s) 435
MluI ACGCGT 1 cut(s) 340
MlyI GAGTC 2 cut(s) 608, 722
MroI TCCGGA 1 cut(s) 503
MseI TTAA 3 cut(s) 129, 438, 784
MslI CAYNNNNRTG 1 cut(s) 325
MspA1I CMGCKG 2 cut(s) 107, 116
MspI CCGG 3 cut(s) 309, 504, 751
MspR9I CCNGG 2 cut(s) 309, 310
Mva1269I GAATGC 1 cut(s) 332
MvnI CGCG 1 cut(s) 342
MwoI GCNNNNNNNGC 6 cut(s) 32, 65, 101, 110, 113, 116
NciI CCSGG 2 cut(s) 309, 310
NdeI CATATG 2 cut(s) 322, 415
NdeII GATC 2 cut(s) 45, 563
NlaIV GGNNCC 4 cut(s) 157, 368, 635, 749
PaeR7I CTCGAG 1 cut(s) 352
PciSI GCTCTTC 1 cut(s) 752
PctI GAATGC 1 cut(s) 332
PfeI GAWTC 5 cut(s) 500, 698, 707, 725, 731
PflMI CCANNNNNTGG 1 cut(s) 165
PleI GAGTC 2 cut(s) 608, 721
PpsI GAGTC 2 cut(s) 608, 721
PpuMI RGGWCCY 2 cut(s) 155, 366
Psp124BI GAGCTC 1 cut(s) 91
Psp5II RGGWCCY 2 cut(s) 155, 366
PspFI CCCAGC 2 cut(s) 158, 369
PspN4I GGNNCC 4 cut(s) 157, 368, 635, 749
PspPI GGNCC 2 cut(s) 155, 366
PspPPI RGGWCCY 2 cut(s) 155, 366
PstI CTGCAG 1 cut(s) 115
PstNI CAGNNNCTG 3 cut(s) 110, 497, 566
PsuI RGATCY 1 cut(s) 563
PvuII CAGCTG 2 cut(s) 107, 116
RsaI GTAC 2 cut(s) 211, 683
RsaNI GTAC 2 cut(s) 210, 682
RseI CAYNNNNRTG 1 cut(s) 325
SacI GAGCTC 1 cut(s) 91
SapI GCTCTTC 1 cut(s) 752
SaqAI TTAA 3 cut(s) 129, 438, 784
Sau3AI GATC 2 cut(s) 45, 563
Sau96I GGNCC 2 cut(s) 155, 366
ScaI AGTACT 1 cut(s) 211
SchI GAGTC 2 cut(s) 608, 722
ScrFI CCNGG 2 cut(s) 309, 310
SduI GDGCHC 2 cut(s) 91, 764
SfaNI GCATC 2 cut(s) 493, 557
SfcI CTRYAG 1 cut(s) 111
Sfr274I CTCGAG 1 cut(s) 352
SinI GGWCC 2 cut(s) 155, 366
SlaI CTCGAG 1 cut(s) 352
SmaI CCCGGG 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 325
SmlI CTYRAG 4 cut(s) 27, 61, 248, 352
SmoI CTYRAG 4 cut(s) 27, 61, 248, 352
Sse9I AATT 1 cut(s) 435
SsiI CCGC 1 cut(s) 35
SspI AATATT 1 cut(s) 572
SspMI CTAG 1 cut(s) 363
SstI GAGCTC 1 cut(s) 91
StyD4I CCNGG 2 cut(s) 307, 308
TaaI ACNGT 3 cut(s) 268, 360, 428
TaqI TCGA 2 cut(s) 85, 353
TasI AATT 1 cut(s) 435
TatI WGTACW 1 cut(s) 209
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 5 cut(s) 500, 698, 707, 725, 731
Tru1I TTAA 3 cut(s) 129, 438, 784
Tru9I TTAA 3 cut(s) 129, 438, 784
TspDTI ATGAA 3 cut(s) 339, 351, 413
TspGWI ACGGA 1 cut(s) 253
TspMI CCCGGG 1 cut(s) 308
Van91I CCANNNNNTGG 1 cut(s) 165
VpaK11BI GGWCC 2 cut(s) 155, 366
XhoI CTCGAG 1 cut(s) 352
XmaI CCCGGG 1 cut(s) 308
XmiI GTMKAC 1 cut(s) 263
XspI CTAG 1 cut(s) 363
ZrmI AGTACT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.