MD13G1172700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016307
Physical Location & Seq
Reverse (-)
14169248 .. 14170465
1218 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1172700.v1.1.491

Sequence Viewer

Length: 735 bp
ATGGGTTGCGGGGAATCAAAGCTGGCGGTTGCCACCACCAACACCATCCTCCGCCGCAAGAAATCGAGTGTTGCCGATCCTTCCAAGAAGAGCAAAGACATAGAAATCGTCCTTGAAAACAATGACAGCGCCGCAAACTCATCGGTGCAACACCAACAACAACATGACCAACAAGAAGAACAAGTCAAGGAGGACAATGCGATTGCCAATAATGTTGGTGGTGGCGAGACGTCTGCGGTGGCCGATGATGTGAATGTGAATGTAAAGGACATAAAGGTAAATAACAAGGACGGAGAAGATGGTGGTAAGGAAGTATTGGAAAAAGGTGACAAAGAAGAGCACGACGGGGCCGCGGGGAGATTGATTTCGCATGGTTCTCCGAATCGTTTCTTTTCGTCGAGGAAATTGGATGAGGAGGGAATTGACGCGATTATTTCTGAGGGACGGTCTGGGACGTCGGACTATTATACTCCACGCCATGGAAGTAAGGGAAACTTGCACTTCAAGGTAGATGATGATATTGCAGAGGACGATAACGAATTAGATCTGCCGGCTGCAGAAACAAAAGCTCCAGTGGTGGAGACACAAAATAAGGGTACAGAAGAGCCTGTAAACAAACCGGAGGAGAATTCGGTGAAGGAAACAGAAGTGGCAGTTACAATCACTGTTGAAGCCGAAGTGGCTGAAGCCGCTGAACCTGAAGTTTCAATCCCTGCTGAAGAAGTGAAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

245

Amino Acids

26.24

Weight (kDa)

4.55

Isoelectric Point (pI)

41.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g17371
malus_domestica MD13G1172700.v1.1 MD16G1174200.v1.1
prunus_persica Prupe.1G140900_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0421301
rosa_laevigata RLG00000007699
rosa_multiflora Rmu_sc0004892.1_g000010 Rmu_sc0007721.1_g000001
rosa_roxburghii Rroxscaffold_5G00363530
rosa_rugosa Rorug04G0169200
rosa_samantha Rh4AG230600 Rh4BG233600 Rh4CG244800 Rh4DG229300
rosa_wichuraiana Rw4G020320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 233, 458
AccB7I CCANNNNNTGG 1 cut(s) 479
AccII CGCG 2 cut(s) 353, 428
AciI CCGC 9 cut(s) 9, 26, 52, 55, 132, 236, 351, 353, 690
AclWI GGATC 1 cut(s) 71
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 1 cut(s) 628
AcuI CTGAAG 2 cut(s) 705, 720
AcyI GRCGYC 2 cut(s) 230, 455
AfaI GTAC 1 cut(s) 598
AfiI CCNNNNNNNGG 1 cut(s) 479
AgsI TTSAA 4 cut(s) 116, 505, 671, 708
AluBI AGCT 2 cut(s) 22, 569
AluI AGCT 2 cut(s) 22, 569
Alw21I GWGCWC 1 cut(s) 342
Alw26I GTCTC 2 cut(s) 221, 575
AlwI GGATC 1 cut(s) 71
AoxI GGCC 2 cut(s) 240, 348
ApeKI GCWGC 1 cut(s) 554
ApoI RAATTY 1 cut(s) 628
Asp700I GAANNNNTTC 1 cut(s) 386
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 1 cut(s) 348
AsuHPI GGTGA 2 cut(s) 338, 646
Bbv12I GWGCWC 1 cut(s) 342
BbvI GCAGC 1 cut(s) 541
BccI CCATC 2 cut(s) 53, 293
BcgI CGANNNNNNTGC 4 cut(s) 123, 157, 215, 249
BcoDI GTCTC 2 cut(s) 221, 575
BfmI CTRYAG 1 cut(s) 555
BfoI RGCGCY 1 cut(s) 132
BglI GCCNNNNNGGC 1 cut(s) 680
BglII AGATCT 1 cut(s) 544
BisI GCNGC 5 cut(s) 55, 132, 351, 555, 690
BlsI GCNGC 5 cut(s) 56, 133, 352, 556, 691
BmgT120I GGNCC 1 cut(s) 348
BmiI GGNNCC 1 cut(s) 349
BpmI CTGGAG 1 cut(s) 555
BsaHI GRCGYC 2 cut(s) 230, 455
BsaJI CCNNGG 2 cut(s) 351, 478
BsaWI WCCGGW 1 cut(s) 619
BsaXI ACNNNNNCTCC 2 cut(s) 553, 583
Bsc4I CCNNNNNNNGG 1 cut(s) 479
Bse118I RCCGGY 1 cut(s) 550
Bse1I ACTGG 1 cut(s) 572
BseDI CCNNGG 2 cut(s) 351, 478
BseGI GGATG 2 cut(s) 45, 415
BseLI CCNNNNNNNGG 1 cut(s) 479
BseMII CTCAG 1 cut(s) 429
BseNI ACTGG 1 cut(s) 572
BseRI GAGGAG 2 cut(s) 428, 638
BseXI GCAGC 1 cut(s) 541
Bsh1236I CGCG 2 cut(s) 353, 428
BshFI GGCC 2 cut(s) 242, 350
BsiHKAI GWGCWC 1 cut(s) 342
BsiSI CCGG 2 cut(s) 551, 620
BslFI GGGAC 2 cut(s) 456, 466
BslI CCNNNNNNNGG 1 cut(s) 479
BsmAI GTCTC 2 cut(s) 221, 575
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 2 cut(s) 456, 466
BsnI GGCC 2 cut(s) 242, 350
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 2 cut(s) 76, 544
Bsp19I CCATGG 1 cut(s) 478
BspACI CCGC 9 cut(s) 9, 26, 52, 55, 132, 236, 351, 353, 690
BspANI GGCC 2 cut(s) 242, 350
BspCNI CTCAG 1 cut(s) 430
BspFNI CGCG 2 cut(s) 353, 428
BspLI GGNNCC 1 cut(s) 349
BspMAI CTGCAG 1 cut(s) 559
BspPI GGATC 1 cut(s) 71
BspQI GCTCTTC 3 cut(s) 83, 330, 597
BsrFI RCCGGY 1 cut(s) 550
BsrI ACTGG 1 cut(s) 572
BssAI RCCGGY 1 cut(s) 550
BssECI CCNNGG 2 cut(s) 351, 478
BssMI GATC 2 cut(s) 76, 544
BssNI GRCGYC 2 cut(s) 230, 455
BssT1I CCWWGG 1 cut(s) 478
Bst4CI ACNGT 2 cut(s) 447, 667
Bst6I CTCTTC 3 cut(s) 83, 330, 597
BstACI GRCGYC 2 cut(s) 230, 455
BstC8I GCNNGC 2 cut(s) 24, 552
BstDEI CTNAG 1 cut(s) 438
BstDSI CCRYGG 2 cut(s) 351, 478
BstF5I GGATG 2 cut(s) 45, 415
BstFNI CGCG 2 cut(s) 353, 428
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 2 cut(s) 79, 547
BstMAI GTCTC 2 cut(s) 221, 575
BstMBI GATC 2 cut(s) 76, 544
BstMWI GCNNNNNNNGC 2 cut(s) 680, 689
BstSFI CTRYAG 1 cut(s) 555
BstUI CGCG 2 cut(s) 353, 428
BstV1I GCAGC 1 cut(s) 541
BstX2I RGATCY 1 cut(s) 544
BstYI RGATCY 1 cut(s) 544
BsuRI GGCC 2 cut(s) 242, 350
BtgI CCRYGG 2 cut(s) 351, 478
BtsCI GGATG 2 cut(s) 45, 415
BtsIMutI CAGTG 2 cut(s) 579, 663
Cac8I GCNNGC 2 cut(s) 24, 552
CfoI GCGC 1 cut(s) 131
Cfr10I RCCGGY 1 cut(s) 550
Cfr13I GGNCC 1 cut(s) 348
Cfr42I CCGCGG 1 cut(s) 354
CseI GACGC 1 cut(s) 434
Csp6I GTAC 1 cut(s) 597
CviAII CATG 3 cut(s) 164, 371, 479
CviJI RGCY 9 cut(s) 22, 242, 350, 554, 569, 607, 674, 683, 689
CviKI_1 RGCY 9 cut(s) 22, 242, 350, 554, 569, 607, 674, 683, 689
CviQI GTAC 1 cut(s) 597
DdeI CTNAG 1 cut(s) 438
DpnI GATC 2 cut(s) 78, 546
DpnII GATC 2 cut(s) 76, 544
EaeI YGGCCR 1 cut(s) 240
Eam1104I CTCTTC 3 cut(s) 83, 330, 597
EarI CTCTTC 3 cut(s) 83, 330, 597
EciI GGCGGA 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 478
Eco57I CTGAAG 2 cut(s) 705, 720
EcoRI GAATTC 1 cut(s) 628
EcoT14I CCWWGG 1 cut(s) 478
ErhI CCWWGG 1 cut(s) 478
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 3 cut(s) 167, 374, 482
FaiI YATR 6 cut(s) 101, 165, 272, 372, 468, 480
FalI AAGNNNNNCTT 2 cut(s) 479, 511
FaqI GGGAC 2 cut(s) 456, 466
FatI CATG 3 cut(s) 163, 370, 478
FauI CCCGC 2 cut(s) 2, 346
Fnu4HI GCNGC 5 cut(s) 55, 132, 351, 555, 690
FokI GGATG 2 cut(s) 32, 422
Fsp4HI GCNGC 5 cut(s) 55, 132, 351, 555, 690
GlaI GCGC 1 cut(s) 130
GluI GCNGC 5 cut(s) 55, 132, 351, 555, 690
GsuI CTGGAG 1 cut(s) 555
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 2 cut(s) 242, 350
HapII CCGG 2 cut(s) 551, 620
HgaI GACGC 1 cut(s) 434
HhaI GCGC 1 cut(s) 131
Hin1I GRCGYC 2 cut(s) 230, 455
Hin1II CATG 3 cut(s) 167, 374, 482
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HinfI GANTC 2 cut(s) 14, 382
HpaII CCGG 2 cut(s) 551, 620
HphI GGTGA 2 cut(s) 338, 646
Hpy166II GTNNAC 1 cut(s) 613
Hpy188I TCNGA 3 cut(s) 381, 439, 460
Hpy8I GTNNAC 1 cut(s) 613
Hpy99I CGWCG 3 cut(s) 347, 400, 460
HpyAV CCTTC 2 cut(s) 90, 631
HpyCH4III ACNGT 2 cut(s) 447, 667
HpyCH4IV ACGT 2 cut(s) 230, 455
HpyCH4V TGCA 4 cut(s) 148, 499, 524, 557
HpyF10VI GCNNNNNNNGC 2 cut(s) 680, 689
HpyF3I CTNAG 1 cut(s) 438
HpySE526I ACGT 2 cut(s) 230, 455
Hsp92I GRCGYC 2 cut(s) 230, 455
Hsp92II CATG 3 cut(s) 167, 374, 482
HspAI GCGC 1 cut(s) 129
KroI GCCGGC 1 cut(s) 550
KroNI GCCGGC 1 cut(s) 552
KspI CCGCGG 1 cut(s) 354
Kzo9I GATC 2 cut(s) 76, 544
LguI GCTCTTC 3 cut(s) 83, 330, 597
LmnI GCTCC 1 cut(s) 574
LpnPI CCDG 8 cut(s) 8, 435, 564, 585, 621, 633, 711, 726
Lsp1109I GCAGC 1 cut(s) 541
MaeII ACGT 2 cut(s) 230, 455
MaeIII GTNAC 2 cut(s) 326, 655
MalI GATC 2 cut(s) 78, 546
MboI GATC 2 cut(s) 76, 544
MboII GAAGA 6 cut(s) 100, 188, 308, 347, 614, 731
MflI RGATCY 1 cut(s) 544
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 5 cut(s) 404, 420, 539, 628, 730
MmeI TCCRAC 1 cut(s) 438
MnlI CCTC 8 cut(s) 59, 184, 393, 406, 409, 433, 520, 616
MroNI GCCGGC 1 cut(s) 550
MroXI GAANNNNTTC 1 cut(s) 386
MseI TTAA 1 cut(s) 733
MspA1I CMGCKG 2 cut(s) 353, 692
MspI CCGG 2 cut(s) 551, 620
MvnI CGCG 2 cut(s) 353, 428
MwoI GCNNNNNNNGC 2 cut(s) 680, 689
NaeI GCCGGC 1 cut(s) 552
NcoI CCATGG 1 cut(s) 478
NdeII GATC 2 cut(s) 76, 544
NgoMIV GCCGGC 1 cut(s) 550
NlaIII CATG 3 cut(s) 167, 374, 482
NlaIV GGNNCC 1 cut(s) 349
NmuCI GTSAC 1 cut(s) 326
PciSI GCTCTTC 3 cut(s) 83, 330, 597
PdiI GCCGGC 1 cut(s) 552
PdmI GAANNNNTTC 1 cut(s) 386
PfeI GAWTC 2 cut(s) 14, 382
PflMI CCANNNNNTGG 1 cut(s) 479
PkrI GCNGC 5 cut(s) 56, 133, 352, 556, 691
PspN4I GGNNCC 1 cut(s) 349
PspPI GGNCC 1 cut(s) 348
PstI CTGCAG 1 cut(s) 559
PsuI RGATCY 1 cut(s) 544
RsaI GTAC 1 cut(s) 598
RsaNI GTAC 1 cut(s) 597
SacII CCGCGG 1 cut(s) 354
SapI GCTCTTC 3 cut(s) 83, 330, 597
SaqAI TTAA 1 cut(s) 733
SatI GCNGC 5 cut(s) 55, 132, 351, 555, 690
Sau3AI GATC 2 cut(s) 76, 544
Sau96I GGNCC 1 cut(s) 348
SduI GDGCHC 1 cut(s) 342
SetI ASST 8 cut(s) 24, 233, 279, 328, 458, 510, 571, 700
SfcI CTRYAG 1 cut(s) 555
Sfr303I CCGCGG 1 cut(s) 354
SgrBI CCGCGG 1 cut(s) 354
Sse9I AATT 5 cut(s) 404, 420, 539, 628, 730
SsiI CCGC 9 cut(s) 9, 26, 52, 55, 132, 236, 351, 353, 690
StyI CCWWGG 1 cut(s) 478
TaaI ACNGT 2 cut(s) 447, 667
TaiI ACGT 2 cut(s) 233, 458
TaqI TCGA 2 cut(s) 65, 398
TasI AATT 5 cut(s) 404, 420, 539, 628, 730
TauI GCSGC 4 cut(s) 57, 134, 353, 692
TfiI GAWTC 2 cut(s) 14, 382
Tru1I TTAA 1 cut(s) 733
Tru9I TTAA 1 cut(s) 733
TscAI CASTG 2 cut(s) 579, 670
TseFI GTSAC 1 cut(s) 326
TseI GCWGC 1 cut(s) 554
Tsp45I GTSAC 1 cut(s) 326
TspGWI ACGGA 1 cut(s) 306
TspRI CASTG 2 cut(s) 579, 670
Van91I CCANNNNNTGG 1 cut(s) 479
XapI RAATTY 1 cut(s) 628
XmnI GAANNNNTTC 1 cut(s) 386
ZraI GACGTC 2 cut(s) 231, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.