MD13G1193000.v1.1

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008379
Physical Location & Seq
Forward (+)
16719226 .. 16724241
5016 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1193000.v1.1.491

Sequence Viewer

Length: 681 bp
ATGCATTATTTCAGGTCCTCTAAAGATTTTCCAGATATGCATAGTCTCATCATGCACAGTTACAACTATGATAGTGCTGACTTGCGCGTGGATCACCTGGGGTTCCACAAGGCTTTGTGTGTTCTAATGGGTTGGGACTACTTGAAGCCTCCCGAGAACTCAAGGGCGTATCAGTTTCTTTCTGCTGAAGCAGCAGCAGCAAATGTGGATGATCTGATTATGTGGCCACCTGTGGTGATAATTCATAATACTGTTACGGGAAAGAGTAAAGATGGCCGCATGGAGGGTTTGGGCAATAAAGCAATGGATAGTAATATTAGAGATCTTGGATTTGGAAGTGGGAAGTCGAAATCCTTATATGGTAGAGATGGTCATTTGGGTATGACTCTGGTTAAGTTTTCCGGTGACGAAGCAGGCCTAAAGGATGCCATTCGAATGGCAGAGTTCTTTGAGAAGGAAAATCATGGGCGCAGGGGCTGGGCTCGTGTACAGCCGCCAACGCTGGGCAGTAGGGATGATGATAACAATCCAAACCTTGTCAAGTTTGATGAAAAGACGCGGGAGAAGAAGAGGATCCTATATGGCTATCTCGGAACAGCTTATGATCTTGACAAAGTTGATTTCGACACAAGAAAGAAGGCCGTGATCGAAAGCCTTAGGGAGTACAAGTCATCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

227

Amino Acids

25.69

Weight (kDa)

8.46

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 70 - 204 4.7e-27 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 87, 559
AciI CCGC 3 cut(s) 277, 494, 559
AclWI GGATC 3 cut(s) 99, 568, 581
AcoI YGGCCR 2 cut(s) 224, 274
AcuI CTGAAG 1 cut(s) 207
AfaI GTAC 2 cut(s) 489, 665
AfiI CCNNNNNNNGG 2 cut(s) 283, 503
AgsI TTSAA 1 cut(s) 145
AjnI CCWGG 1 cut(s) 96
AluBI AGCT 1 cut(s) 599
AluI AGCT 1 cut(s) 599
Alw26I GTCTC 1 cut(s) 50
AlwI GGATC 3 cut(s) 99, 568, 581
AlwNI CAGNNNCTG 1 cut(s) 477
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 4 cut(s) 224, 274, 415, 639
ApeKI GCWGC 3 cut(s) 191, 194, 197
AspLEI GCGC 2 cut(s) 87, 471
AspS9I GGNCC 1 cut(s) 15
AsuHPI GGTGA 3 cut(s) 86, 247, 416
AsuII TTCGAA 1 cut(s) 433
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 1 cut(s) 15
AxyI CCTNAGG 1 cut(s) 656
BalI TGGCCA 1 cut(s) 226
BamHI GGATCC 1 cut(s) 573
BanII GRGCYC 1 cut(s) 484
BauI CACGAG 1 cut(s) 483
BbvI GCAGC 3 cut(s) 203, 206, 209
BccI CCATC 2 cut(s) 266, 362
BceAI ACGGC 1 cut(s) 626
BciT130I CCWGG 1 cut(s) 98
BcoDI GTCTC 1 cut(s) 50
BglII AGATCT 1 cut(s) 322
BisI GCNGC 5 cut(s) 192, 195, 198, 277, 494
BlsI GCNGC 5 cut(s) 193, 196, 199, 278, 495
Bme1390I CCNGG 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 15
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 1 cut(s) 15
BmiI GGNNCC 2 cut(s) 104, 575
BmrFI CCNGG 1 cut(s) 98
BmsI GCATC 1 cut(s) 415
Bpu14I TTCGAA 1 cut(s) 433
BpuEI CTTGAG 1 cut(s) 145
BsaBI GATNNNNATC 1 cut(s) 525
BsaJI CCNNGG 1 cut(s) 97
BsaWI WCCGGW 1 cut(s) 401
BsaXI ACNNNNNCTCC 1 cut(s) 653
Bsc4I CCNNNNNNNGG 2 cut(s) 283, 503
Bse21I CCTNAGG 1 cut(s) 656
Bse3DI GCAATG 1 cut(s) 309
Bse8I GATNNNNATC 1 cut(s) 525
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 1 cut(s) 97
BseGI GGATG 4 cut(s) 214, 430, 520, 671
BseJI GATNNNNATC 1 cut(s) 525
BseLI CCNNNNNNNGG 2 cut(s) 283, 503
BseMI GCAATG 1 cut(s) 309
BseXI GCAGC 3 cut(s) 203, 206, 209
BseYI CCCAGC 2 cut(s) 477, 502
Bsh1236I CGCG 2 cut(s) 87, 559
BshFI GGCC 4 cut(s) 226, 276, 417, 641
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 1 cut(s) 402
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 2 cut(s) 283, 503
BsmAI GTCTC 1 cut(s) 50
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 4 cut(s) 226, 276, 417, 641
BsoBI CYCGRG 1 cut(s) 152
Bsp119I TTCGAA 1 cut(s) 433
Bsp1286I GDGCHC 1 cut(s) 484
Bsp1407I TGTACA 1 cut(s) 487
Bsp143I GATC 6 cut(s) 91, 211, 322, 573, 604, 645
BspACI CCGC 3 cut(s) 277, 494, 559
BspANI GGCC 4 cut(s) 226, 276, 417, 641
BspFNI CGCG 2 cut(s) 87, 559
BspLI GGNNCC 2 cut(s) 104, 575
BspPI GGATC 3 cut(s) 99, 568, 581
BspT104I TTCGAA 1 cut(s) 433
BsrDI GCAATG 1 cut(s) 309
BsrGI TGTACA 1 cut(s) 487
BssECI CCNNGG 1 cut(s) 97
BssMI GATC 6 cut(s) 91, 211, 322, 573, 604, 645
BssSI CACGAG 1 cut(s) 483
Bst2BI CACGAG 1 cut(s) 483
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 2 cut(s) 59, 253
Bst6I CTCTTC 1 cut(s) 563
BstAUI TGTACA 1 cut(s) 487
BstBI TTCGAA 1 cut(s) 433
BstC8I GCNNGC 1 cut(s) 415
BstDEI CTNAG 1 cut(s) 656
BstF5I GGATG 4 cut(s) 214, 430, 520, 671
BstFNI CGCG 2 cut(s) 87, 559
BstHHI GCGC 2 cut(s) 87, 471
BstKTI GATC 6 cut(s) 94, 214, 325, 576, 607, 648
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 6 cut(s) 91, 211, 322, 573, 604, 645
BstMWI GCNNNNNNNGC 3 cut(s) 191, 197, 499
BstNI CCWGG 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 96
BstUI CGCG 2 cut(s) 87, 559
BstV1I GCAGC 3 cut(s) 203, 206, 209
BstX2I RGATCY 2 cut(s) 322, 573
BstXI CCANNNNNNTGG 1 cut(s) 436
BstYI RGATCY 2 cut(s) 322, 573
Bsu36I CCTNAGG 1 cut(s) 656
BsuRI GGCC 4 cut(s) 226, 276, 417, 641
BtsCI GGATG 4 cut(s) 214, 430, 520, 671
Cac8I GCNNGC 1 cut(s) 415
CaiI CAGNNNCTG 1 cut(s) 477
CfoI GCGC 2 cut(s) 87, 471
Cfr13I GGNCC 1 cut(s) 15
CseI GACGC 1 cut(s) 565
Csp6I GTAC 2 cut(s) 488, 664
CviAII CATG 3 cut(s) 52, 280, 464
CviQI GTAC 2 cut(s) 488, 664
DdeI CTNAG 1 cut(s) 656
DpnI GATC 6 cut(s) 93, 213, 324, 575, 606, 647
DpnII GATC 6 cut(s) 91, 211, 322, 573, 604, 645
EaeI YGGCCR 2 cut(s) 224, 274
Eam1104I CTCTTC 1 cut(s) 563
EarI CTCTTC 1 cut(s) 563
Eco147I AGGCCT 1 cut(s) 417
Eco24I GRGCYC 1 cut(s) 484
Eco47I GGWCC 1 cut(s) 15
Eco57I CTGAAG 1 cut(s) 207
Eco81I CCTNAGG 1 cut(s) 656
Eco88I CYCGRG 1 cut(s) 152
EcoO109I RGGNCCY 1 cut(s) 15
EcoRII CCWGG 1 cut(s) 96
EcoT22I ATGCAT 2 cut(s) 6, 42
EcoT38I GRGCYC 1 cut(s) 484
FaeI CATG 3 cut(s) 55, 283, 467
FaqI GGGAC 1 cut(s) 149
FatI CATG 3 cut(s) 51, 279, 463
FauI CCCGC 1 cut(s) 552
Fnu4HI GCNGC 5 cut(s) 192, 195, 198, 277, 494
FokI GGATG 4 cut(s) 221, 437, 527, 658
FriOI GRGCYC 1 cut(s) 484
Fsp4HI GCNGC 5 cut(s) 192, 195, 198, 277, 494
GlaI GCGC 2 cut(s) 86, 470
GluI GCNGC 5 cut(s) 192, 195, 198, 277, 494
GsaI CCCAGC 2 cut(s) 481, 506
HaeIII GGCC 4 cut(s) 226, 276, 417, 641
HapII CCGG 1 cut(s) 402
HgaI GACGC 1 cut(s) 565
HhaI GCGC 2 cut(s) 87, 471
Hin1II CATG 3 cut(s) 55, 283, 467
Hin6I GCGC 2 cut(s) 85, 469
HinP1I GCGC 2 cut(s) 85, 469
HinfI GANTC 1 cut(s) 385
HpaII CCGG 1 cut(s) 402
HphI GGTGA 3 cut(s) 86, 247, 416
Hpy166II GTNNAC 1 cut(s) 488
Hpy188I TCNGA 2 cut(s) 216, 593
Hpy188III TCNNGA 3 cut(s) 32, 152, 608
Hpy8I GTNNAC 1 cut(s) 488
HpyAV CCTTC 2 cut(s) 448, 631
HpyCH4III ACNGT 2 cut(s) 59, 253
HpyCH4V TGCA 3 cut(s) 4, 40, 55
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 197, 499
HpyF3I CTNAG 1 cut(s) 656
Hsp92II CATG 3 cut(s) 55, 283, 467
HspAI GCGC 2 cut(s) 85, 469
Kzo9I GATC 6 cut(s) 91, 211, 322, 573, 604, 645
Lsp1109I GCAGC 3 cut(s) 203, 206, 209
LweI GCATC 1 cut(s) 415
MaeIII GTNAC 3 cut(s) 59, 253, 404
MalI GATC 6 cut(s) 93, 213, 324, 575, 606, 647
MboI GATC 6 cut(s) 91, 211, 322, 573, 604, 645
MboII GAAGA 2 cut(s) 577, 580
MflI RGATCY 2 cut(s) 322, 573
MhlI GDGCHC 1 cut(s) 484
MlsI TGGCCA 1 cut(s) 226
MluCI AATT 1 cut(s) 240
MluNI TGGCCA 1 cut(s) 226
MlyI GAGTC 1 cut(s) 379
MnlI CCTC 4 cut(s) 28, 159, 277, 564
Mox20I TGGCCA 1 cut(s) 226
Mph1103I ATGCAT 2 cut(s) 6, 42
MscI TGGCCA 1 cut(s) 226
MseI TTAA 1 cut(s) 393
MslI CAYNNNNRTG 1 cut(s) 434
Msp20I TGGCCA 1 cut(s) 226
MspI CCGG 1 cut(s) 402
MspR9I CCNGG 1 cut(s) 98
MvaI CCWGG 1 cut(s) 98
MvnI CGCG 2 cut(s) 87, 559
MwoI GCNNNNNNNGC 3 cut(s) 191, 197, 499
NdeII GATC 6 cut(s) 91, 211, 322, 573, 604, 645
NlaIII CATG 3 cut(s) 55, 283, 467
NlaIV GGNNCC 2 cut(s) 104, 575
NmuCI GTSAC 1 cut(s) 404
NsiI ATGCAT 2 cut(s) 6, 42
NspV TTCGAA 1 cut(s) 433
PceI AGGCCT 1 cut(s) 417
PkrI GCNGC 5 cut(s) 193, 196, 199, 278, 495
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
PpuMI RGGWCCY 1 cut(s) 15
Psp5II RGGWCCY 1 cut(s) 15
Psp6I CCWGG 1 cut(s) 96
PspFI CCCAGC 2 cut(s) 477, 502
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 2 cut(s) 104, 575
PspPI GGNCC 1 cut(s) 15
PspPPI RGGWCCY 1 cut(s) 15
PstNI CAGNNNCTG 1 cut(s) 477
PsuI RGATCY 2 cut(s) 322, 573
RsaI GTAC 2 cut(s) 489, 665
RsaNI GTAC 2 cut(s) 488, 664
RseI CAYNNNNRTG 1 cut(s) 434
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 5 cut(s) 192, 195, 198, 277, 494
Sau3AI GATC 6 cut(s) 91, 211, 322, 573, 604, 645
Sau96I GGNCC 1 cut(s) 15
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 1 cut(s) 98
SduI GDGCHC 1 cut(s) 484
SetI ASST 5 cut(s) 17, 99, 232, 537, 601
SfaNI GCATC 1 cut(s) 415
SfuI TTCGAA 1 cut(s) 433
SinI GGWCC 1 cut(s) 15
SmiMI CAYNNNNRTG 1 cut(s) 434
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
Sse9I AATT 1 cut(s) 240
SseBI AGGCCT 1 cut(s) 417
SsiI CCGC 3 cut(s) 277, 494, 559
SspI AATATT 1 cut(s) 316
StuI AGGCCT 1 cut(s) 417
StyD4I CCNGG 1 cut(s) 96
TaaI ACNGT 2 cut(s) 59, 253
TaqI TCGA 4 cut(s) 347, 433, 624, 648
TasI AATT 1 cut(s) 240
TatI WGTACW 2 cut(s) 487, 663
TauI GCSGC 2 cut(s) 279, 496
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 3 cut(s) 191, 194, 197
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 2 cut(s) 233, 564
VpaK11BI GGWCC 1 cut(s) 15
Zsp2I ATGCAT 2 cut(s) 6, 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.