Rh4BG199400

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0008379
Physical Location & Seq
Reverse (-)
34285084 .. 34295684
10601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG199400.1

Sequence Viewer

Length: 1590 bp
ATGGCCGGAGGTAACCACCCCAAGGCCCCACCACACAACAACAAGCCTTCCTCTTCTTCCTCCGCCTCTAACCGCAAATCCCGCTGGGAATCCTCCCCCTCCACCACCATCAAAAACAACCACCCCCACCCACCCGACCCGACCAAACCCCCCAAGCCCACCACCACCGGCCCATCCCCCAATAAGGCCCCCACCGCCAGCCCCGCCAACCCCAAGCACCCCCCGCTCCCCTCTCCCGGCGCCGCCCCTCCCTTTCCCTTCCCGGACCCCGCCGCCTTCGGCCCGCCGCCTCCGCCCGTCTACGGCTTCCACAACCTCGAGCGCCGCACGATCGTTCTCGCCGACGGTAGCGTCCGGTCCTACTTCGCCCTCCCGCCAGATTACCAGGACTTCCCGCCTCCTCCTCCTCCGCATATGGACCCGTCGGGGCGGTTCTTGCCGTTCGGGCCGGGCCGGCCGGCCGCCCCGGACTACTGGAATTCGCTTGGGCTGGACGGCCGTGGGCCCCAGGAAGGGTCGATGAAGAGGAAGTTTGGGGAAGAAGAGGAGCTGAGGGATAAGGGAGAAGAGTTGGCCAAGCGGCGGCAGCAGTTGGTGCAATTCGGAAACGGGTTTGGGGCGGGTCCGAGTAGCCCGTTTCGGCGTGACTTGGGGGCCCAATCCAGAAGTGGCGATGATCCGAGGGCTTCCAAGTATATGAGGACTGGTGGGGGTTATGAGAATGTGGGGTTTAGGCAAGGCGGTGGCGGCGGAGGTGGTGATAATGTGGGTCATAAGCATCTTCAGGTTGACCAGGCTGCGTTGAAGAAGGCATTTCTGTACTTTGCGAAGGTGATTAATGAGAATGGAGCGCAGAAGAAGATTTACATAGAGGATGGGAAGCAGGGACGGCTTAATTGTCTTGCTTGTGGCACCACTGGCAGGTCTGCAAAAGATTTTCCGGATATGCATAGTCTCATCATGCACAGTTACAACACGGACAATGCGGACATTCGTGTGGATCACTTGGGCTTACACAAGGCTTTGTGTGTTTTAAAGGGGTGGAACTACTCAAAGCCTCCTGATAACTCAAAGGCATATCAGTTCCTTTCCGCTGATGAAGCAGCAGCAAATCAAGATGACTTGATTATGTGGCCGCCCATGGTGATAATTCATAATACGCTTACAGGAAAGAGTAAAGATGGCCGCATGGAGGGTTTGGGCAATAAAGCTATGGATAGTTATATACGAGATCTTGGGTTTGGGAGTGGCAAGTCGAAGTCCCTGTACGGTAGAGATGGACATTTGGGAACAACTTTAATCAAGTTTTCAGCTGATGAAGCGGGCCTCAAGGAGGCCTTGCGAATGACAGAATTCTTTGAGAAGGACAATCATGGACGCAGGGCTTGGTATCATGTACAACCGCTCATGTTGGGCAATAAGGATGATGAGAACAATCCTTATCTTGTGAAGTTAGATGAAAAGACTCGGGAAAAGAAGAGAGTCCTTTATGGTTATCTAGGAACTGCTTCTGATCTTGAGAAAGTTGATTTTGACACGAGAAAGAAGGCTGTGATTGAAAGCTGGAGAGAATTCAAACCTCCTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

529

Amino Acids

58.08

Weight (kDa)

9.35

Isoelectric Point (pI)

52.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 373 - 507 4.4e-27 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 350
Acc36I ACCTGC 1 cut(s) 912
AccB1I GGYRCC 2 cut(s) 239, 911
AccBSI CCGCTC 2 cut(s) 226, 1405
AccI GTMKAC 1 cut(s) 300
AccIII TCCGGA 1 cut(s) 940
AclWI GGATC 2 cut(s) 671, 1008
AcoI YGGCCR 7 cut(s) 3, 455, 459, 496, 573, 1133, 1183
AcsI RAATTY 3 cut(s) 478, 1352, 1571
AcuI CTGAAG 1 cut(s) 767
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 3 cut(s) 821, 1268, 1398
AgsI TTSAA 3 cut(s) 805, 1559, 1576
AjnI CCWGG 3 cut(s) 384, 507, 792
AluBI AGCT 4 cut(s) 550, 1211, 1313, 1563
AluI AGCT 4 cut(s) 550, 1211, 1313, 1563
Alw26I GTCTC 1 cut(s) 959
AlwI GGATC 2 cut(s) 671, 1008
Ama87I CYCGRG 2 cut(s) 317, 1467
Aor13HI TCCGGA 1 cut(s) 940
ApaI GGGCCC 2 cut(s) 507, 658
ApeKI GCWGC 4 cut(s) 586, 797, 1103, 1106
ApoI RAATTY 3 cut(s) 478, 1352, 1571
AseI ATTAAT 1 cut(s) 837
Asp700I GAANNNNTTC 1 cut(s) 1507
AspLEI GCGC 3 cut(s) 242, 324, 853
AsuC2I CCSGG 4 cut(s) 237, 263, 450, 467
AsuHPI GGTGA 3 cut(s) 770, 844, 1156
AvaI CYCGRG 2 cut(s) 317, 1467
AvaII GGWCC 4 cut(s) 265, 357, 418, 623
BaeGI GKGCMC 2 cut(s) 507, 658
BalI TGGCCA 1 cut(s) 575
BanI GGYRCC 2 cut(s) 239, 911
BanII GRGCYC 2 cut(s) 507, 658
BarI GAAGNNNNNNTAC 4 cut(s) 848, 880, 1250, 1282
BauI CACGAG 1 cut(s) 1537
BbvCI CCTCAGC 1 cut(s) 551
BbvI GCAGC 4 cut(s) 598, 784, 1115, 1118
BccI CCATC 5 cut(s) 116, 181, 869, 1175, 1271
BceAI ACGGC 5 cut(s) 319, 424, 483, 511, 905
BciT130I CCWGG 3 cut(s) 386, 509, 794
BcnI CCSGG 4 cut(s) 237, 263, 450, 467
BcoDI GTCTC 1 cut(s) 959
BfaI CTAG 1 cut(s) 1499
BfoI RGCGCY 2 cut(s) 243, 325
BfuAI ACCTGC 1 cut(s) 912
BglI GCCNNNNNGGC 2 cut(s) 445, 454
BglII AGATCT 1 cut(s) 1231
Bme1390I CCNGG 7 cut(s) 237, 263, 386, 450, 467, 509, 794
Bme18I GGWCC 4 cut(s) 265, 357, 418, 623
BmeT110I CYCGRG 2 cut(s) 317, 1467
BmrFI CCNGG 7 cut(s) 237, 263, 386, 450, 467, 509, 794
BmsI GCATC 1 cut(s) 787
BpmI CTGGAG 1 cut(s) 1585
Bpu10I CCTNAGC 1 cut(s) 551
BpuEI CTTGAG 2 cut(s) 1313, 1538
BpuMI CCSGG 4 cut(s) 237, 263, 450, 467
BsaHI GRCGYC 1 cut(s) 240
BsaJI CCNNGG 6 cut(s) 21, 465, 499, 507, 680, 1140
BsaWI WCCGGW 2 cut(s) 354, 940
Bse118I RCCGGY 3 cut(s) 167, 453, 457
Bse1I ACTGG 3 cut(s) 479, 709, 922
BseAI TCCGGA 1 cut(s) 940
BseBI CCWGG 3 cut(s) 386, 509, 794
BseDI CCNNGG 6 cut(s) 21, 465, 499, 507, 680, 1140
BseGI GGATG 3 cut(s) 173, 880, 1429
BseMII CTCAG 1 cut(s) 542
BseNI ACTGG 3 cut(s) 479, 709, 922
BseRI GAGGAG 4 cut(s) 390, 393, 396, 560
BseSI GKGCMC 2 cut(s) 507, 658
BseX3I CGGCCG 3 cut(s) 455, 459, 496
BseXI GCAGC 4 cut(s) 598, 784, 1115, 1118
BseYI CCCAGC 1 cut(s) 84
Bsh1285I CGRYCG 4 cut(s) 333, 458, 462, 499
BshNI GGYRCC 2 cut(s) 239, 911
BsiEI CGRYCG 4 cut(s) 333, 458, 462, 499
BsiHKCI CYCGRG 2 cut(s) 317, 1467
BslFI GGGAC 2 cut(s) 900, 1246
BsmAI GTCTC 1 cut(s) 959
BsmFI GGGAC 2 cut(s) 900, 1246
BsoBI CYCGRG 2 cut(s) 317, 1467
Bsp120I GGGCCC 2 cut(s) 503, 654
Bsp1286I GDGCHC 2 cut(s) 507, 658
Bsp13I TCCGGA 1 cut(s) 940
Bsp1407I TGTACA 1 cut(s) 1396
Bsp143I GATC 5 cut(s) 330, 676, 1000, 1231, 1513
Bsp19I CCATGG 1 cut(s) 1140
BspCNI CTCAG 1 cut(s) 543
BspEI TCCGGA 1 cut(s) 940
BspMI ACCTGC 1 cut(s) 912
BspPI GGATC 2 cut(s) 671, 1008
BspT107I GGYRCC 2 cut(s) 239, 911
BsrBI CCGCTC 2 cut(s) 226, 1405
BsrFI RCCGGY 3 cut(s) 167, 453, 457
BsrGI TGTACA 1 cut(s) 1396
BsrI ACTGG 3 cut(s) 479, 709, 922
BssAI RCCGGY 3 cut(s) 167, 453, 457
BssECI CCNNGG 6 cut(s) 21, 465, 499, 507, 680, 1140
BssMI GATC 5 cut(s) 330, 676, 1000, 1231, 1513
BssNI GRCGYC 1 cut(s) 240
BssSI CACGAG 1 cut(s) 1537
BssT1I CCWWGG 2 cut(s) 21, 1140
Bst2BI CACGAG 1 cut(s) 1537
Bst2UI CCWGG 3 cut(s) 386, 509, 794
Bst4CI ACNGT 3 cut(s) 347, 968, 1271
Bst6I CTCTTC 5 cut(s) 58, 518, 537, 561, 1472
BstACI GRCGYC 1 cut(s) 240
BstAPI GCANNNNNTGC 1 cut(s) 595
BstAUI TGTACA 1 cut(s) 1396
BstC8I GCNNGC 5 cut(s) 199, 284, 455, 459, 1324
BstDEI CTNAG 2 cut(s) 551, 1584
BstDSI CCRYGG 2 cut(s) 499, 1140
BstEII GGTNACC 1 cut(s) 11
BstENI CCTNNNNNAGG 1 cut(s) 1331
BstF5I GGATG 3 cut(s) 173, 880, 1429
BstH2I RGCGCY 2 cut(s) 243, 325
BstHHI GCGC 3 cut(s) 242, 324, 853
BstKTI GATC 5 cut(s) 333, 679, 1003, 1234, 1516
BstMAI GTCTC 1 cut(s) 959
BstMBI GATC 5 cut(s) 330, 676, 1000, 1231, 1513
BstMCI CGRYCG 4 cut(s) 333, 458, 462, 499
BstNI CCWGG 3 cut(s) 386, 509, 794
BstPI GGTNACC 1 cut(s) 11
BstSCI CCNGG 7 cut(s) 235, 261, 384, 448, 465, 507, 792
BstSLI GKGCMC 2 cut(s) 507, 658
BstV1I GCAGC 4 cut(s) 598, 784, 1115, 1118
BstX2I RGATCY 1 cut(s) 1231
BstYI RGATCY 1 cut(s) 1231
BstZI CGGCCG 3 cut(s) 455, 459, 496
BtgI CCRYGG 2 cut(s) 499, 1140
BtgZI GCGATG 1 cut(s) 687
BtsCI GGATG 3 cut(s) 173, 880, 1429
BtsIMutI CAGTG 1 cut(s) 915
BveI ACCTGC 1 cut(s) 912
Cac8I GCNNGC 5 cut(s) 199, 284, 455, 459, 1324
CfoI GCGC 3 cut(s) 242, 324, 853
Cfr10I RCCGGY 3 cut(s) 167, 453, 457
CseI GACGC 2 cut(s) 340, 1386
Csp6I GTAC 3 cut(s) 820, 1267, 1397
CviAII CATG 6 cut(s) 961, 1141, 1189, 1373, 1394, 1408
CviQI GTAC 3 cut(s) 820, 1267, 1397
DdeI CTNAG 2 cut(s) 551, 1584
DinI GGCGCC 1 cut(s) 241
DpnI GATC 5 cut(s) 332, 678, 1002, 1233, 1515
DpnII GATC 5 cut(s) 330, 676, 1000, 1231, 1513
DraI TTTAAA 1 cut(s) 1035
DrdI GACNNNNNNGTC 1 cut(s) 350
DseDI GACNNNNNNGTC 1 cut(s) 350
EaeI YGGCCR 7 cut(s) 3, 455, 459, 496, 573, 1133, 1183
EagI CGGCCG 3 cut(s) 455, 459, 496
Eam1104I CTCTTC 5 cut(s) 58, 518, 537, 561, 1472
EarI CTCTTC 5 cut(s) 58, 518, 537, 561, 1472
EciI GGCGGA 3 cut(s) 52, 282, 765
EclXI CGGCCG 3 cut(s) 455, 459, 496
Eco130I CCWWGG 2 cut(s) 21, 1140
Eco147I AGGCCT 1 cut(s) 1337
Eco24I GRGCYC 2 cut(s) 507, 658
Eco47I GGWCC 4 cut(s) 265, 357, 418, 623
Eco52I CGGCCG 3 cut(s) 455, 459, 496
Eco57I CTGAAG 1 cut(s) 767
Eco88I CYCGRG 2 cut(s) 317, 1467
Eco91I GGTNACC 1 cut(s) 11
EcoNI CCTNNNNNAGG 1 cut(s) 1331
EcoO109I RGGNCCY 4 cut(s) 25, 187, 504, 654
EcoO65I GGTNACC 1 cut(s) 11
EcoRI GAATTC 3 cut(s) 478, 1352, 1571
EcoRII CCWGG 3 cut(s) 384, 507, 792
EcoT14I CCWWGG 2 cut(s) 21, 1140
EcoT22I ATGCAT 1 cut(s) 951
EcoT38I GRGCYC 2 cut(s) 507, 658
EgeI GGCGCC 1 cut(s) 241
EheI GGCGCC 1 cut(s) 241
ErhI CCWWGG 2 cut(s) 21, 1140
FaeI CATG 6 cut(s) 964, 1144, 1192, 1376, 1397, 1411
FalI AAGNNNNNCTT 2 cut(s) 1322, 1354
FaqI GGGAC 2 cut(s) 900, 1246
FatI CATG 6 cut(s) 960, 1140, 1188, 1372, 1393, 1407
FauI CCCGC 9 cut(s) 89, 211, 231, 277, 291, 381, 402, 613, 1315
FauNDI CATATG 1 cut(s) 414
FblI GTMKAC 1 cut(s) 300
FokI GGATG 3 cut(s) 160, 887, 1436
FriOI GRGCYC 2 cut(s) 507, 658
FseI GGCCGGCC 2 cut(s) 457, 461
FspBI CTAG 1 cut(s) 1499
GlaI GCGC 3 cut(s) 241, 323, 852
GsaI CCCAGC 1 cut(s) 88
GsuI CTGGAG 1 cut(s) 1585
HaeII RGCGCY 2 cut(s) 243, 325
HgaI GACGC 2 cut(s) 340, 1386
HhaI GCGC 3 cut(s) 242, 324, 853
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 6 cut(s) 964, 1144, 1192, 1376, 1397, 1411
Hin6I GCGC 3 cut(s) 240, 322, 851
HinP1I GCGC 3 cut(s) 240, 322, 851
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HinfI GANTC 3 cut(s) 89, 1465, 1482
HphI GGTGA 3 cut(s) 770, 844, 1156
Hpy166II GTNNAC 2 cut(s) 301, 790
Hpy188I TCNGA 4 cut(s) 605, 627, 681, 1513
Hpy188III TCNNGA 6 cut(s) 663, 941, 1061, 1115, 1469, 1517
Hpy8I GTNNAC 2 cut(s) 301, 790
Hpy99I CGWCG 2 cut(s) 347, 427
HpyAV CCTTC 8 cut(s) 57, 268, 286, 506, 802, 823, 1357, 1540
HpyCH4III ACNGT 3 cut(s) 347, 968, 1271
HpyCH4V TGCA 4 cut(s) 598, 929, 949, 964
HpyF3I CTNAG 2 cut(s) 551, 1584
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 6 cut(s) 964, 1144, 1192, 1376, 1397, 1411
HspAI GCGC 3 cut(s) 240, 322, 851
KasI GGCGCC 1 cut(s) 239
Kpn2I TCCGGA 1 cut(s) 940
KroI GCCGGC 2 cut(s) 453, 457
KroNI GCCGGC 2 cut(s) 455, 459
Kzo9I GATC 5 cut(s) 330, 676, 1000, 1231, 1513
LmnI GCTCC 3 cut(s) 231, 547, 848
Lsp1109I GCAGC 4 cut(s) 598, 784, 1115, 1118
LweI GCATC 1 cut(s) 787
MaeI CTAG 1 cut(s) 1499
MaeIII GTNAC 3 cut(s) 11, 644, 968
MalI GATC 5 cut(s) 332, 678, 1002, 1233, 1515
MbiI CCGCTC 2 cut(s) 226, 1405
MboI GATC 5 cut(s) 330, 676, 1000, 1231, 1513
MflI RGATCY 1 cut(s) 1231
MhlI GDGCHC 2 cut(s) 507, 658
MlsI TGGCCA 1 cut(s) 575
MluCI AATT 6 cut(s) 478, 599, 895, 1149, 1352, 1571
MluNI TGGCCA 1 cut(s) 575
Mly113I GGCGCC 1 cut(s) 240
MlyI GAGTC 2 cut(s) 1459, 1491
Mox20I TGGCCA 1 cut(s) 575
Mph1103I ATGCAT 1 cut(s) 951
MroI TCCGGA 1 cut(s) 940
MroNI GCCGGC 2 cut(s) 453, 457
MroXI GAANNNNTTC 1 cut(s) 1507
MscI TGGCCA 1 cut(s) 575
MseI TTAA 4 cut(s) 837, 894, 1034, 1298
MslI CAYNNNNRTG 1 cut(s) 995
Msp20I TGGCCA 1 cut(s) 575
MspA1I CMGCKG 3 cut(s) 84, 1094, 1313
MspR9I CCNGG 7 cut(s) 237, 263, 386, 450, 467, 509, 794
MvaI CCWGG 3 cut(s) 386, 509, 794
NaeI GCCGGC 2 cut(s) 455, 459
NarI GGCGCC 1 cut(s) 240
NciI CCSGG 4 cut(s) 237, 263, 450, 467
NcoI CCATGG 1 cut(s) 1140
NdeI CATATG 1 cut(s) 414
NdeII GATC 5 cut(s) 330, 676, 1000, 1231, 1513
NgoMIV GCCGGC 2 cut(s) 453, 457
NlaIII CATG 6 cut(s) 964, 1144, 1192, 1376, 1397, 1411
NmuCI GTSAC 1 cut(s) 644
NsiI ATGCAT 1 cut(s) 951
PaeR7I CTCGAG 1 cut(s) 317
PceI AGGCCT 1 cut(s) 1337
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PdiI GCCGGC 2 cut(s) 455, 459
PdmI GAANNNNTTC 1 cut(s) 1507
PfeI GAWTC 1 cut(s) 89
PfoI TCCNGGA 1 cut(s) 261
Ple19I CGATCG 1 cut(s) 333
PleI GAGTC 2 cut(s) 1459, 1490
PluTI GGCGCC 1 cut(s) 243
PpsI GAGTC 2 cut(s) 1459, 1490
PshBI ATTAAT 1 cut(s) 837
Psp6I CCWGG 3 cut(s) 384, 507, 792
PspEI GGTNACC 1 cut(s) 11
PspFI CCCAGC 1 cut(s) 84
PspGI CCWGG 3 cut(s) 384, 507, 792
PspOMI GGGCCC 2 cut(s) 503, 654
PspXI VCTCGAGB 1 cut(s) 317
PsuI RGATCY 1 cut(s) 1231
PvuI CGATCG 1 cut(s) 333
PvuII CAGCTG 1 cut(s) 1313
RigI GGCCGGCC 2 cut(s) 457, 461
RsaI GTAC 3 cut(s) 821, 1268, 1398
RsaNI GTAC 3 cut(s) 820, 1267, 1397
RseI CAYNNNNRTG 1 cut(s) 995
SaqAI TTAA 4 cut(s) 837, 894, 1034, 1298
Sau3AI GATC 5 cut(s) 330, 676, 1000, 1231, 1513
SchI GAGTC 2 cut(s) 1459, 1491
ScrFI CCNGG 7 cut(s) 237, 263, 386, 450, 467, 509, 794
SduI GDGCHC 2 cut(s) 507, 658
SfaNI GCATC 1 cut(s) 787
SfiI GGCCNNNNNGGCC 1 cut(s) 454
SfoI GGCGCC 1 cut(s) 241
Sfr274I CTCGAG 1 cut(s) 317
SinI GGWCC 4 cut(s) 265, 357, 418, 623
SlaI CTCGAG 1 cut(s) 317
SmiMI CAYNNNNRTG 1 cut(s) 995
SmlI CTYRAG 3 cut(s) 317, 1328, 1517
SmoI CTYRAG 3 cut(s) 317, 1328, 1517
Sse9I AATT 6 cut(s) 478, 599, 895, 1149, 1352, 1571
SseBI AGGCCT 1 cut(s) 1337
SspDI GGCGCC 1 cut(s) 239
SspMI CTAG 1 cut(s) 1499
StuI AGGCCT 1 cut(s) 1337
StyD4I CCNGG 7 cut(s) 235, 261, 384, 448, 465, 507, 792
StyI CCWWGG 2 cut(s) 21, 1140
TaaI ACNGT 3 cut(s) 347, 968, 1271
TaqI TCGA 3 cut(s) 318, 518, 1256
TasI AATT 6 cut(s) 478, 599, 895, 1149, 1352, 1571
TatI WGTACW 2 cut(s) 819, 1396
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 4 cut(s) 837, 894, 1034, 1298
Tru9I TTAA 4 cut(s) 837, 894, 1034, 1298
TscAI CASTG 1 cut(s) 922
TseFI GTSAC 1 cut(s) 644
TseI GCWGC 4 cut(s) 586, 797, 1103, 1106
Tsp45I GTSAC 1 cut(s) 644
TspDTI ATGAA 5 cut(s) 536, 1113, 1142, 1332, 1473
TspGWI ACGGA 1 cut(s) 992
TspRI CASTG 1 cut(s) 922
VpaK11BI GGWCC 4 cut(s) 265, 357, 418, 623
VspI ATTAAT 1 cut(s) 837
XagI CCTNNNNNAGG 1 cut(s) 1331
XapI RAATTY 3 cut(s) 478, 1352, 1571
XcmI CCANNNNNNNNNTGG 1 cut(s) 665
XhoI CTCGAG 1 cut(s) 317
XmiI GTMKAC 1 cut(s) 300
XmnI GAANNNNTTC 1 cut(s) 1507
XspI CTAG 1 cut(s) 1499
Zsp2I ATGCAT 1 cut(s) 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.