pycom16g16360

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008379
Physical Location & Seq
Forward (+)
12298519 .. 12304379
5861 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g16360.2

Sequence Viewer

Length: 1254 bp
ATGGAAGATTCGAAGCAAAAGGCCCCATTGCGCATCCGTAAAAACAAAACTTATAAAATAAACTCCCAGCGACAGGAAGCATCGTCTTCCTCATTCCTCCTCTCCCACTTTCACACTCTCAATTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCTTCTTCTCCTATTTAATTTATTTATTTTCCTCCAAATCCCTAACCCCAGGATGGCTGGAGGTCCCCATCCCAAGGGCCCATCACTCCACAAGCCATCTTCTTCCTCCGCCTCCAGCCGCAAATCGCGCTGGGAATCCTCCGCCAACGCCACCGCCCCCACCGCCACCCCCAGCACCACCACCATCACCAACAACAAAAACCCACCCGATCCCAAATCCTCCAAGACCAAAACCGGCCCATCCCCAAAACCGAGCCCCGCCCATCCCAAGCACCCGCCTGGCCCCTCTCCCGGAACCGCTCCATTCCCCTTCTCCGACCCGGCTGCTTTCGGCCCACCACCTCCCCCGGTATACGATTTCCACATGCTCGAGCGCCGGACTTTTGTTCTTGCCGATGGTAGTGCTCGATCCTACTTCGCTCTCCCGCCCGATTATCAAGACTTCCCCCCACCCATGAACCCGCCTGGCCGGTTCATGCCATTTGGTCCGGGTGGATCGGGCGGGCCGGGTCCGGATTACTGGAATTCGCTCGGGCTCGACGGGCGTGGGCCTGCAGAAGGGCCCGCTAAGAGGAAGTACAGCGGAGAAAAGGAGCAGAGGGATAAGGCAGAAGAATTGGCAATGCGTCGGCAGCAACTTATGCAGTATGGGAATCCAAATGGGTTTTCGGGGGGTCCCGGAGGAGGTCGGGCAACGAGTAGTCCCTTTCGGCGGGAAGCGGGCGGCGATGAGGGGAGGGCTAACAAGTACATGAGGGTTGGTGGTGGTGGTGGAGGTCATGAGAGTGTGGGGTTTAGGCAAGGCGGTGGTGGCGGAGGTGAGAATGTGGGTCTGAAGCATCTTCAAGTTGATCAGACTGCGTTGAAGAAGGCGTTTCTTAACTTTGTGAAGCTGATTCATGAGAACACGCAGCAGAGGAAGACTTACTTGGAGGATGGGAAGCAAGGCCGTCTGCATTGTCTTGCTTGTGTCAGTATTTCAGGTCCTCGAAAGATTTTCCGGATATGCATAGTCTCATCATGCACAGTTACAACTCTGATAGTGCTGACATGCGCGTGGATCACTTGGGGTTACACAAGGCTTTGTGTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

418

Amino Acids

44.97

Weight (kDa)

9.97

Isoelectric Point (pI)

55.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 54
Acc16I TGCGCA 1 cut(s) 32
AccBSI CCGCTC 1 cut(s) 458
AccI GTMKAC 1 cut(s) 510
AccII CGCG 2 cut(s) 286, 1214
AccIII TCCGGA 2 cut(s) 670, 1158
AclWI GGATC 4 cut(s) 362, 561, 661, 1226
AcoI YGGCCR 1 cut(s) 625
AcsI RAATTY 1 cut(s) 682
AcuI CTGAAG 1 cut(s) 1013
AfaI GTAC 2 cut(s) 737, 908
AfiI CCNNNNNNNGG 8 cut(s) 73, 211, 232, 449, 716, 729, 842, 870
AgsI TTSAA 2 cut(s) 1004, 1024
AjnI CCWGG 3 cut(s) 206, 436, 622
AjuI GAANNNNNNNTTGG 4 cut(s) 808, 840, 1070, 1102
AluBI AGCT 1 cut(s) 1051
AluI AGCT 1 cut(s) 1051
Alw21I GWGCWC 1 cut(s) 565
Alw26I GTCTC 1 cut(s) 1177
AlwI GGATC 4 cut(s) 362, 561, 661, 1226
Ama87I CYCGRG 2 cut(s) 527, 689
Aor13HI TCCGGA 2 cut(s) 670, 1158
ApaI GGGCCC 2 cut(s) 239, 723
ApeKI GCWGC 3 cut(s) 482, 790, 1069
ApoI RAATTY 1 cut(s) 682
AspLEI GCGC 4 cut(s) 33, 288, 534, 1214
AsuC2I CCSGG 6 cut(s) 450, 479, 506, 648, 666, 837
AsuHPI GGTGA 2 cut(s) 337, 989
AsuII TTCGAA 1 cut(s) 11
AvaI CYCGRG 2 cut(s) 527, 689
AvaII GGWCC 5 cut(s) 221, 644, 668, 833, 1142
BaeGI GKGCMC 2 cut(s) 239, 723
BanII GRGCYC 4 cut(s) 239, 416, 696, 723
BbsI GAAGAC 2 cut(s) 78, 1085
Bbv12I GWGCWC 1 cut(s) 565
BbvI GCAGC 3 cut(s) 469, 802, 1081
BccI CCATC 9 cut(s) 205, 234, 247, 262, 350, 406, 429, 548, 1088
BceAI ACGGC 1 cut(s) 1092
BcgI CGANNNNNNTGC 4 cut(s) 464, 498, 542, 576
BciT130I CCWGG 3 cut(s) 208, 438, 624
BclI TGATCA 1 cut(s) 1009
BcnI CCSGG 6 cut(s) 450, 479, 506, 648, 666, 837
BcoDI GTCTC 1 cut(s) 1177
BfmI CTRYAG 1 cut(s) 711
BfoI RGCGCY 1 cut(s) 535
BisI GCNGC 5 cut(s) 277, 483, 791, 883, 1070
BlsI GCNGC 5 cut(s) 278, 484, 792, 884, 1071
Bme1390I CCNGG 9 cut(s) 208, 438, 450, 479, 506, 624, 648, 666, 837
Bme18I GGWCC 5 cut(s) 221, 644, 668, 833, 1142
BmeT110I CYCGRG 2 cut(s) 527, 689
BmiI GGNNCC 9 cut(s) 24, 223, 237, 442, 454, 669, 721, 834, 835
BmrFI CCNGG 9 cut(s) 208, 438, 450, 479, 506, 624, 648, 666, 837
BmsI GCATC 3 cut(s) 42, 89, 1006
BpiI GAAGAC 2 cut(s) 78, 1085
BpmI CTGGAG 2 cut(s) 237, 256
Bpu14I TTCGAA 1 cut(s) 11
BpuMI CCSGG 6 cut(s) 450, 479, 506, 648, 666, 837
BsaJI CCNNGG 3 cut(s) 206, 231, 504
BsaWI WCCGGW 2 cut(s) 670, 1158
Bsc4I CCNNNNNNNGG 8 cut(s) 73, 211, 232, 449, 716, 729, 842, 870
Bse118I RCCGGY 2 cut(s) 392, 627
Bse1I ACTGG 1 cut(s) 683
Bse3DI GCAATG 2 cut(s) 26, 786
BseAI TCCGGA 2 cut(s) 670, 1158
BseBI CCWGG 3 cut(s) 208, 438, 624
BseDI CCNNGG 3 cut(s) 206, 231, 504
BseGI GGATG 6 cut(s) 33, 216, 226, 398, 421, 1099
BseLI CCNNNNNNNGG 8 cut(s) 73, 211, 232, 449, 716, 729, 842, 870
BseMI GCAATG 2 cut(s) 26, 786
BseNI ACTGG 1 cut(s) 683
BseRI GAGGAG 2 cut(s) 89, 855
BseSI GKGCMC 2 cut(s) 239, 723
BseXI GCAGC 3 cut(s) 469, 802, 1081
BseYI CCCAGC 3 cut(s) 66, 288, 329
Bsh1236I CGCG 2 cut(s) 286, 1214
BsiHKAI GWGCWC 1 cut(s) 565
BsiHKCI CYCGRG 2 cut(s) 527, 689
BslFI GGGAC 3 cut(s) 207, 819, 846
BslI CCNNNNNNNGG 8 cut(s) 73, 211, 232, 449, 716, 729, 842, 870
BsmAI GTCTC 1 cut(s) 1177
BsmFI GGGAC 3 cut(s) 207, 819, 846
BsoBI CYCGRG 2 cut(s) 527, 689
Bsp119I TTCGAA 1 cut(s) 11
Bsp120I GGGCCC 2 cut(s) 235, 719
Bsp1286I GDGCHC 5 cut(s) 239, 416, 565, 696, 723
Bsp13I TCCGGA 2 cut(s) 670, 1158
Bsp143I GATC 5 cut(s) 367, 566, 653, 1009, 1218
BspEI TCCGGA 2 cut(s) 670, 1158
BspFNI CGCG 2 cut(s) 286, 1214
BspHI TCATGA 2 cut(s) 937, 1057
BspLI GGNNCC 9 cut(s) 24, 223, 237, 442, 454, 669, 721, 834, 835
BspMAI CTGCAG 1 cut(s) 715
BspPI GGATC 4 cut(s) 362, 561, 661, 1226
BspT104I TTCGAA 1 cut(s) 11
BsrBI CCGCTC 1 cut(s) 458
BsrDI GCAATG 2 cut(s) 26, 786
BsrFI RCCGGY 2 cut(s) 392, 627
BsrI ACTGG 1 cut(s) 683
BssAI RCCGGY 2 cut(s) 392, 627
BssECI CCNNGG 3 cut(s) 206, 231, 504
BssMI GATC 5 cut(s) 367, 566, 653, 1009, 1218
BssNAI GTATAC 1 cut(s) 511
BssT1I CCWWGG 1 cut(s) 231
Bst1107I GTATAC 1 cut(s) 511
Bst2UI CCWGG 3 cut(s) 208, 438, 624
Bst4CI ACNGT 1 cut(s) 1186
BstAPI GCANNNNNTGC 1 cut(s) 799
BstBI TTCGAA 1 cut(s) 11
BstC8I GCNNGC 4 cut(s) 662, 711, 723, 880
BstDEI CTNAG 1 cut(s) 726
BstENI CCTNNNNNAGG 1 cut(s) 714
BstF5I GGATG 6 cut(s) 33, 216, 226, 398, 421, 1099
BstFNI CGCG 2 cut(s) 286, 1214
BstH2I RGCGCY 1 cut(s) 535
BstHHI GCGC 4 cut(s) 33, 288, 534, 1214
BstKTI GATC 5 cut(s) 370, 569, 656, 1012, 1221
BstMAI GTCTC 1 cut(s) 1177
BstMBI GATC 5 cut(s) 367, 566, 653, 1009, 1218
BstMWI GCNNNNNNNGC 6 cut(s) 285, 320, 700, 790, 799, 969
BstNI CCWGG 3 cut(s) 208, 438, 624
BstNSI RCATGY 2 cut(s) 526, 1212
BstSCI CCNGG 9 cut(s) 206, 436, 448, 477, 504, 622, 646, 664, 835
BstSFI CTRYAG 1 cut(s) 711
BstSLI GKGCMC 2 cut(s) 239, 723
BstUI CGCG 2 cut(s) 286, 1214
BstV1I GCAGC 3 cut(s) 469, 802, 1081
BstV2I GAAGAC 2 cut(s) 78, 1085
BstZ17I GTATAC 1 cut(s) 511
BtgZI GCGATG 1 cut(s) 900
BtsCI GGATG 6 cut(s) 33, 216, 226, 398, 421, 1099
Cac8I GCNNGC 4 cut(s) 662, 711, 723, 880
CciI TCATGA 2 cut(s) 937, 1057
CfoI GCGC 4 cut(s) 33, 288, 534, 1214
Cfr10I RCCGGY 2 cut(s) 392, 627
CseI GACGC 1 cut(s) 773
Csp6I GTAC 2 cut(s) 736, 907
CviAII CATG 8 cut(s) 523, 613, 634, 910, 938, 1058, 1179, 1209
CviQI GTAC 2 cut(s) 736, 907
DdeI CTNAG 1 cut(s) 726
DpnI GATC 5 cut(s) 369, 568, 655, 1011, 1220
DpnII GATC 5 cut(s) 367, 566, 653, 1009, 1218
EaeI YGGCCR 1 cut(s) 625
EciI GGCGGA 3 cut(s) 256, 289, 987
Eco130I CCWWGG 1 cut(s) 231
Eco24I GRGCYC 4 cut(s) 239, 416, 696, 723
Eco47I GGWCC 5 cut(s) 221, 644, 668, 833, 1142
Eco57I CTGAAG 1 cut(s) 1013
Eco88I CYCGRG 2 cut(s) 527, 689
EcoNI CCTNNNNNAGG 1 cut(s) 714
EcoO109I RGGNCCY 6 cut(s) 22, 221, 235, 719, 833, 1142
EcoRI GAATTC 1 cut(s) 682
EcoRII CCWGG 3 cut(s) 206, 436, 622
EcoT14I CCWWGG 1 cut(s) 231
EcoT22I ATGCAT 1 cut(s) 1169
EcoT38I GRGCYC 4 cut(s) 239, 416, 696, 723
ErhI CCWWGG 1 cut(s) 231
FaeI CATG 8 cut(s) 526, 616, 637, 913, 941, 1061, 1182, 1212
FalI AAGNNNNNCTT 2 cut(s) 1070, 1102
FaqI GGGAC 3 cut(s) 207, 819, 846
FatI CATG 8 cut(s) 522, 612, 633, 909, 937, 1057, 1178, 1208
FauI CCCGC 8 cut(s) 424, 441, 591, 627, 653, 730, 864, 871
FbaI TGATCA 1 cut(s) 1009
FblI GTMKAC 1 cut(s) 510
Fnu4HI GCNGC 5 cut(s) 277, 483, 791, 883, 1070
FokI GGATG 6 cut(s) 20, 213, 223, 385, 408, 1106
FriOI GRGCYC 4 cut(s) 239, 416, 696, 723
Fsp4HI GCNGC 5 cut(s) 277, 483, 791, 883, 1070
FspI TGCGCA 1 cut(s) 32
GlaI GCGC 4 cut(s) 32, 287, 533, 1213
GluI GCNGC 5 cut(s) 277, 483, 791, 883, 1070
GsaI CCCAGC 3 cut(s) 70, 292, 333
GsuI CTGGAG 2 cut(s) 237, 256
HaeII RGCGCY 1 cut(s) 535
HgaI GACGC 1 cut(s) 773
HhaI GCGC 4 cut(s) 33, 288, 534, 1214
Hin1II CATG 8 cut(s) 526, 616, 637, 913, 941, 1061, 1182, 1212
Hin6I GCGC 4 cut(s) 31, 286, 532, 1212
HinP1I GCGC 4 cut(s) 31, 286, 532, 1212
HinfI GANTC 4 cut(s) 8, 293, 811, 1054
HphI GGTGA 2 cut(s) 337, 989
Hpy166II GTNNAC 1 cut(s) 511
Hpy188I TCNGA 4 cut(s) 475, 993, 1014, 1197
Hpy188III TCNNGA 5 cut(s) 596, 671, 938, 1058, 1159
Hpy8I GTNNAC 1 cut(s) 511
Hpy99I CGWCG 2 cut(s) 701, 789
HpyAV CCTTC 4 cut(s) 170, 478, 710, 1021
HpyCH4III ACNGT 1 cut(s) 1186
HpyCH4V TGCA 5 cut(s) 713, 802, 1114, 1167, 1182
HpyF10VI GCNNNNNNNGC 6 cut(s) 285, 320, 700, 790, 799, 969
HpyF3I CTNAG 1 cut(s) 726
Hsp92II CATG 8 cut(s) 526, 616, 637, 913, 941, 1061, 1182, 1212
HspAI GCGC 4 cut(s) 31, 286, 532, 1212
KflI GGGWCCC 1 cut(s) 833
Kpn2I TCCGGA 2 cut(s) 670, 1158
Ksp22I TGATCA 1 cut(s) 1009
Kzo9I GATC 5 cut(s) 367, 566, 653, 1009, 1218
LmnI GCTCC 2 cut(s) 463, 751
Lsp1109I GCAGC 3 cut(s) 469, 802, 1081
LweI GCATC 3 cut(s) 42, 89, 1006
MaeIII GTNAC 2 cut(s) 1186, 1229
MalI GATC 5 cut(s) 369, 568, 655, 1011, 1220
MbiI CCGCTC 1 cut(s) 458
MboI GATC 5 cut(s) 367, 566, 653, 1009, 1218
MboII GAAGA 9 cut(s) 17, 78, 155, 249, 252, 782, 992, 1036, 1090
MhlI GDGCHC 5 cut(s) 239, 416, 565, 696, 723
MluCI AATT 4 cut(s) 121, 175, 682, 773
MmeI TCCRAC 1 cut(s) 498
Mph1103I ATGCAT 1 cut(s) 1169
MroI TCCGGA 2 cut(s) 670, 1158
MseI TTAA 2 cut(s) 174, 1038
MslI CAYNNNNRTG 2 cut(s) 942, 1213
MspA1I CMGCKG 1 cut(s) 741
MspR9I CCNGG 9 cut(s) 208, 438, 450, 479, 506, 624, 648, 666, 837
MvaI CCWGG 3 cut(s) 208, 438, 624
MvnI CGCG 2 cut(s) 286, 1214
MwoI GCNNNNNNNGC 6 cut(s) 285, 320, 700, 790, 799, 969
NciI CCSGG 6 cut(s) 450, 479, 506, 648, 666, 837
NdeII GATC 5 cut(s) 367, 566, 653, 1009, 1218
NlaIII CATG 8 cut(s) 526, 616, 637, 913, 941, 1061, 1182, 1212
NlaIV GGNNCC 9 cut(s) 24, 223, 237, 442, 454, 669, 721, 834, 835
NsbI TGCGCA 1 cut(s) 32
NsiI ATGCAT 1 cut(s) 1169
NspI RCATGY 2 cut(s) 526, 1212
NspV TTCGAA 1 cut(s) 11
PaeR7I CTCGAG 1 cut(s) 527
PagI TCATGA 2 cut(s) 937, 1057
PfeI GAWTC 4 cut(s) 8, 293, 811, 1054
PfoI TCCNGGA 2 cut(s) 448, 835
PkrI GCNGC 5 cut(s) 278, 484, 792, 884, 1071
PpuMI RGGWCCY 3 cut(s) 221, 833, 1142
PsiI TTATAA 1 cut(s) 54
Psp5II RGGWCCY 3 cut(s) 221, 833, 1142
Psp6I CCWGG 3 cut(s) 206, 436, 622
PspFI CCCAGC 3 cut(s) 66, 288, 329
PspGI CCWGG 3 cut(s) 206, 436, 622
PspN4I GGNNCC 9 cut(s) 24, 223, 237, 442, 454, 669, 721, 834, 835
PspOMI GGGCCC 2 cut(s) 235, 719
PspPPI RGGWCCY 3 cut(s) 221, 833, 1142
PspXI VCTCGAGB 1 cut(s) 527
PstI CTGCAG 1 cut(s) 715
RsaI GTAC 2 cut(s) 737, 908
RsaNI GTAC 2 cut(s) 736, 907
RseI CAYNNNNRTG 2 cut(s) 942, 1213
SaqAI TTAA 2 cut(s) 174, 1038
SatI GCNGC 5 cut(s) 277, 483, 791, 883, 1070
Sau3AI GATC 5 cut(s) 367, 566, 653, 1009, 1218
ScrFI CCNGG 9 cut(s) 208, 438, 450, 479, 506, 624, 648, 666, 837
SduI GDGCHC 5 cut(s) 239, 416, 565, 696, 723
SetI ASST 7 cut(s) 223, 502, 847, 937, 979, 1053, 1144
SfaNI GCATC 3 cut(s) 42, 89, 1006
SfcI CTRYAG 1 cut(s) 711
Sfr274I CTCGAG 1 cut(s) 527
SfuI TTCGAA 1 cut(s) 11
SinI GGWCC 5 cut(s) 221, 644, 668, 833, 1142
SlaI CTCGAG 1 cut(s) 527
SmiMI CAYNNNNRTG 2 cut(s) 942, 1213
SmlI CTYRAG 1 cut(s) 527
SmoI CTYRAG 1 cut(s) 527
Sse9I AATT 4 cut(s) 121, 175, 682, 773
StyD4I CCNGG 9 cut(s) 206, 436, 448, 477, 504, 622, 646, 664, 835
StyI CCWWGG 1 cut(s) 231
TaaI ACNGT 1 cut(s) 1186
TaqI TCGA 5 cut(s) 11, 528, 565, 696, 1147
TasI AATT 4 cut(s) 121, 175, 682, 773
TatI WGTACW 2 cut(s) 735, 906
TauI GCSGC 2 cut(s) 279, 885
TfiI GAWTC 4 cut(s) 8, 293, 811, 1054
Tru1I TTAA 2 cut(s) 174, 1038
Tru9I TTAA 2 cut(s) 174, 1038
TseI GCWGC 3 cut(s) 482, 790, 1069
TspDTI ATGAA 3 cut(s) 622, 629, 1046
TspGWI ACGGA 1 cut(s) 26
VpaK11BI GGWCC 5 cut(s) 221, 644, 668, 833, 1142
XagI CCTNNNNNAGG 1 cut(s) 714
XapI RAATTY 1 cut(s) 682
XceI RCATGY 2 cut(s) 526, 1212
XhoI CTCGAG 1 cut(s) 527
XmiI GTMKAC 1 cut(s) 510
Zsp2I ATGCAT 1 cut(s) 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.