pycom13g16530

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0008379
Physical Location & Seq
Forward (+)
12346843 .. 12348723
1881 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g16530.2

Sequence Viewer

Length: 1071 bp
ATGGCCGGAGGTCCACCTCCCAAGGGCCCACCACTCCACAAGCCATCTTCTTCTTCCTCCGCCTCCAGCCGCAAATCTCGCTGGGAATCCTACCCCAACGCCACCGCCACCACCACCACCACCACCACCAACAAAAACAAAAACCCACCCGACCCCAAATCCTCCAAGACTAAACCCGGACCATCCCCGAAACCCAGCCCCGCCCATCCCAAAAACCAGCCAGGCCCCTCTCCTAGAGCTGCTTCCTTCCCCTTCTCCGACCCGGCTTCTTTCGGCCCACCACCTCCGCCGGTGTACGATTTCCACATGCTCGAGCGCCGGACTTTTGTTCTTGCCGACGGTAGTGTTCGATCTTACTTCGCTCTGCCGCCAGATTATCAAGAGTTCCCTCCGCCCATGAACCCGCCTGGCCGGTTCATGCCATTCGGTCCCGGTGGACCAGGTGGGCCGGATTACTGGAATTCGCTAGGGCTCGACGGGCGTGGGCCTGCAGAAGGGCCCGCTAAGAGGAAGTACGCCGAAGAAGAGGAGCACAGGGATAAAGCAGAAGAATTGGCGATGCGTCGGCAGCAATTTATGCAGTATGGGAATCCAAATGGTTTTCCGGGGGGTCCCGGAGGAGGAGGTCGGGGAGAGTTTTTGCCGGGATCGAGTAGTCCGTTTCGGCGGGAAGTGGGCGGCAGTGAGGGGAGAGCTAACAAGTACATGAGGGTTGGTGGTGGTGGGGGTGGAGGTCATGAGAGTGTGGGGTTTAGGCAAGTCGGCGGAGGCGGAGGCGAGAATGTGGGTCTGAAGCATCTTCAAGTTGATCAAATTGCGTTGAAGAAGGCGTTTCTTAACTTTGTGAAGCTGATTCATGAGAATACGCAGCAGAGGAAGACTTACTTGGAGGATGGGAAGCATGGCCGTCTGCACTGCATTGCTTGTGCCAGGTTTAAGACATGCTTACACGAGGATCTGTGCTTTATGGTATGTGCTGCATTAGGCTTCAAGGAAAAGTCATTTCACATTTGGTGGATAATGAAGACTGGTTATCTCCTTGGAAGAGAGAATCGTTTTCCCTTTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

357

Amino Acids

38.67

Weight (kDa)

9.67

Isoelectric Point (pI)

60.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 655, 963
AcoI YGGCCR 3 cut(s) 3, 409, 904
AcsI RAATTY 1 cut(s) 460
AcuI CTGAAG 1 cut(s) 812
AfaI GTAC 3 cut(s) 296, 515, 704
AfiI CCNNNNNNNGG 4 cut(s) 23, 494, 507, 620
AgsI TTSAA 3 cut(s) 803, 823, 991
AjnI CCWGG 4 cut(s) 220, 406, 439, 929
AjuI GAANNNNNNNTTGG 2 cut(s) 869, 901
AluBI AGCT 3 cut(s) 239, 695, 850
AluI AGCT 3 cut(s) 239, 695, 850
Alw21I GWGCWC 1 cut(s) 534
AlwI GGATC 2 cut(s) 655, 963
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 9 cut(s) 3, 25, 223, 274, 409, 446, 485, 497, 904
ApaI GGGCCC 2 cut(s) 29, 501
ApeKI GCWGC 4 cut(s) 239, 568, 868, 977
ApoI RAATTY 1 cut(s) 460
AspLEI GCGC 1 cut(s) 318
AsuC2I CCSGG 6 cut(s) 177, 263, 432, 606, 615, 645
AvaI CYCGRG 1 cut(s) 311
AvaII GGWCC 5 cut(s) 11, 179, 428, 437, 611
BaeGI GKGCMC 2 cut(s) 29, 501
BanII GRGCYC 3 cut(s) 29, 474, 501
BauI CACGAG 1 cut(s) 950
BbsI GAAGAC 2 cut(s) 884, 1031
Bbv12I GWGCWC 1 cut(s) 534
BbvI GCAGC 4 cut(s) 226, 580, 880, 964
BccI CCATC 4 cut(s) 52, 190, 213, 887
BceAI ACGGC 1 cut(s) 891
BciT130I CCWGG 4 cut(s) 222, 408, 441, 931
BclI TGATCA 1 cut(s) 808
BcnI CCSGG 6 cut(s) 177, 263, 432, 606, 615, 645
BfaI CTAG 2 cut(s) 234, 467
BfmI CTRYAG 1 cut(s) 489
BfoI RGCGCY 1 cut(s) 319
BisI GCNGC 7 cut(s) 70, 240, 368, 569, 679, 869, 978
BlsI GCNGC 7 cut(s) 71, 241, 369, 570, 680, 870, 979
Bme18I GGWCC 5 cut(s) 11, 179, 428, 437, 611
BmeT110I CYCGRG 1 cut(s) 311
BmiI GGNNCC 6 cut(s) 27, 226, 430, 499, 612, 613
BmsI GCATC 2 cut(s) 549, 805
BpiI GAAGAC 2 cut(s) 884, 1031
BpmI CTGGAG 1 cut(s) 49
BpuMI CCSGG 6 cut(s) 177, 263, 432, 606, 615, 645
BsaJI CCNNGG 3 cut(s) 21, 605, 1039
Bsc4I CCNNNNNNNGG 4 cut(s) 23, 494, 507, 620
Bse118I RCCGGY 2 cut(s) 289, 411
Bse1I ACTGG 2 cut(s) 461, 1033
Bse3DI GCAATG 1 cut(s) 918
BseBI CCWGG 4 cut(s) 222, 408, 441, 931
BseDI CCNNGG 3 cut(s) 21, 605, 1039
BseGI GGATG 3 cut(s) 182, 205, 898
BseLI CCNNNNNNNGG 4 cut(s) 23, 494, 507, 620
BseMI GCAATG 1 cut(s) 918
BseNI ACTGG 2 cut(s) 461, 1033
BseRI GAGGAG 3 cut(s) 542, 633, 636
BseSI GKGCMC 2 cut(s) 29, 501
BseXI GCAGC 4 cut(s) 226, 580, 880, 964
BseYI CCCAGC 2 cut(s) 81, 194
BsgI GTGCAG 1 cut(s) 896
BshFI GGCC 9 cut(s) 5, 27, 225, 276, 411, 448, 487, 499, 906
BsiHKAI GWGCWC 1 cut(s) 534
BsiHKCI CYCGRG 1 cut(s) 311
BslFI GGGAC 2 cut(s) 414, 597
BslI CCNNNNNNNGG 4 cut(s) 23, 494, 507, 620
BsmFI GGGAC 2 cut(s) 414, 597
BsnI GGCC 9 cut(s) 5, 27, 225, 276, 411, 448, 487, 499, 906
BsoBI CYCGRG 1 cut(s) 311
Bsp120I GGGCCC 2 cut(s) 25, 497
Bsp1286I GDGCHC 4 cut(s) 29, 474, 501, 534
Bsp143I GATC 4 cut(s) 350, 647, 808, 955
BspANI GGCC 9 cut(s) 5, 27, 225, 276, 411, 448, 487, 499, 906
BspHI TCATGA 2 cut(s) 736, 856
BspLI GGNNCC 6 cut(s) 27, 226, 430, 499, 612, 613
BspMAI CTGCAG 1 cut(s) 493
BspPI GGATC 2 cut(s) 655, 963
BsrDI GCAATG 1 cut(s) 918
BsrFI RCCGGY 2 cut(s) 289, 411
BsrI ACTGG 2 cut(s) 461, 1033
BssAI RCCGGY 2 cut(s) 289, 411
BssECI CCNNGG 3 cut(s) 21, 605, 1039
BssMI GATC 4 cut(s) 350, 647, 808, 955
BssSI CACGAG 1 cut(s) 950
BssT1I CCWWGG 2 cut(s) 21, 1039
Bst2BI CACGAG 1 cut(s) 950
Bst2UI CCWGG 4 cut(s) 222, 408, 441, 931
Bst4CI ACNGT 1 cut(s) 341
Bst6I CTCTTC 2 cut(s) 519, 1039
BstAPI GCANNNNNTGC 1 cut(s) 577
BstC8I GCNNGC 2 cut(s) 489, 501
BstDEI CTNAG 1 cut(s) 504
BstENI CCTNNNNNAGG 1 cut(s) 492
BstF5I GGATG 3 cut(s) 182, 205, 898
BstH2I RGCGCY 1 cut(s) 319
BstHHI GCGC 1 cut(s) 318
BstKTI GATC 4 cut(s) 353, 650, 811, 958
BstMBI GATC 4 cut(s) 350, 647, 808, 955
BstMWI GCNNNNNNNGC 4 cut(s) 78, 478, 568, 577
BstNI CCWGG 4 cut(s) 222, 408, 441, 931
BstNSI RCATGY 2 cut(s) 310, 945
BstSFI CTRYAG 1 cut(s) 489
BstSLI GKGCMC 2 cut(s) 29, 501
BstV1I GCAGC 4 cut(s) 226, 580, 880, 964
BstV2I GAAGAC 2 cut(s) 884, 1031
BstX2I RGATCY 1 cut(s) 955
BstYI RGATCY 1 cut(s) 955
BsuRI GGCC 9 cut(s) 5, 27, 225, 276, 411, 448, 487, 499, 906
BtgZI GCGATG 1 cut(s) 572
BtsCI GGATG 3 cut(s) 182, 205, 898
BtsI GCAGTG 2 cut(s) 688, 913
BtsIMutI CAGTG 2 cut(s) 688, 913
Cac8I GCNNGC 2 cut(s) 489, 501
CciI TCATGA 2 cut(s) 736, 856
CfoI GCGC 1 cut(s) 318
Cfr10I RCCGGY 2 cut(s) 289, 411
CseI GACGC 1 cut(s) 551
CsiI ACCWGGT 1 cut(s) 439
Csp6I GTAC 3 cut(s) 295, 514, 703
CviAII CATG 8 cut(s) 307, 397, 418, 706, 737, 857, 902, 942
CviQI GTAC 3 cut(s) 295, 514, 703
DdeI CTNAG 1 cut(s) 504
DpnI GATC 4 cut(s) 352, 649, 810, 957
DpnII GATC 4 cut(s) 350, 647, 808, 955
EaeI YGGCCR 3 cut(s) 3, 409, 904
Eam1104I CTCTTC 2 cut(s) 519, 1039
EarI CTCTTC 2 cut(s) 519, 1039
EciI GGCGGA 5 cut(s) 49, 276, 381, 780, 786
Eco130I CCWWGG 2 cut(s) 21, 1039
Eco24I GRGCYC 3 cut(s) 29, 474, 501
Eco47I GGWCC 5 cut(s) 11, 179, 428, 437, 611
Eco57I CTGAAG 1 cut(s) 812
Eco88I CYCGRG 1 cut(s) 311
EcoNI CCTNNNNNAGG 1 cut(s) 492
EcoO109I RGGNCCY 4 cut(s) 25, 224, 497, 611
EcoRI GAATTC 1 cut(s) 460
EcoRII CCWGG 4 cut(s) 220, 406, 439, 929
EcoT14I CCWWGG 2 cut(s) 21, 1039
EcoT38I GRGCYC 3 cut(s) 29, 474, 501
ErhI CCWWGG 2 cut(s) 21, 1039
FaeI CATG 8 cut(s) 310, 400, 421, 709, 740, 860, 905, 945
FalI AAGNNNNNCTT 4 cut(s) 869, 901, 929, 961
FaqI GGGAC 2 cut(s) 414, 597
FatI CATG 8 cut(s) 306, 396, 417, 705, 736, 856, 901, 941
FauI CCCGC 4 cut(s) 208, 411, 508, 660
FbaI TGATCA 1 cut(s) 808
Fnu4HI GCNGC 7 cut(s) 70, 240, 368, 569, 679, 869, 978
FokI GGATG 3 cut(s) 169, 192, 905
FriOI GRGCYC 3 cut(s) 29, 474, 501
Fsp4HI GCNGC 7 cut(s) 70, 240, 368, 569, 679, 869, 978
FspBI CTAG 2 cut(s) 234, 467
GlaI GCGC 1 cut(s) 317
GluI GCNGC 7 cut(s) 70, 240, 368, 569, 679, 869, 978
GsaI CCCAGC 2 cut(s) 85, 198
GsuI CTGGAG 1 cut(s) 49
HaeII RGCGCY 1 cut(s) 319
HaeIII GGCC 9 cut(s) 5, 27, 225, 276, 411, 448, 487, 499, 906
HgaI GACGC 1 cut(s) 551
HhaI GCGC 1 cut(s) 318
Hin1II CATG 8 cut(s) 310, 400, 421, 709, 740, 860, 905, 945
Hin6I GCGC 1 cut(s) 316
HinP1I GCGC 1 cut(s) 316
HinfI GANTC 4 cut(s) 86, 589, 853, 1051
Hpy166II GTNNAC 3 cut(s) 14, 295, 437
Hpy188I TCNGA 2 cut(s) 259, 792
Hpy188III TCNNGA 3 cut(s) 380, 737, 857
Hpy8I GTNNAC 3 cut(s) 14, 295, 437
Hpy99I CGWCG 3 cut(s) 341, 479, 567
HpyAV CCTTC 4 cut(s) 256, 262, 488, 820
HpyCH4III ACNGT 1 cut(s) 341
HpyCH4V TGCA 5 cut(s) 491, 580, 913, 918, 980
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 478, 568, 577
HpyF3I CTNAG 1 cut(s) 504
Hsp92II CATG 8 cut(s) 310, 400, 421, 709, 740, 860, 905, 945
HspAI GCGC 1 cut(s) 316
KflI GGGWCCC 1 cut(s) 611
Ksp22I TGATCA 1 cut(s) 808
Kzo9I GATC 4 cut(s) 350, 647, 808, 955
LmnI GCTCC 1 cut(s) 529
Lsp1109I GCAGC 4 cut(s) 226, 580, 880, 964
LweI GCATC 2 cut(s) 549, 805
MabI ACCWGGT 1 cut(s) 439
MaeI CTAG 2 cut(s) 234, 467
MalI GATC 4 cut(s) 352, 649, 810, 957
MboI GATC 4 cut(s) 350, 647, 808, 955
MflI RGATCY 1 cut(s) 955
MhlI GDGCHC 4 cut(s) 29, 474, 501, 534
MluCI AATT 4 cut(s) 460, 551, 572, 813
MmeI TCCRAC 1 cut(s) 282
MseI TTAA 2 cut(s) 837, 936
MslI CAYNNNNRTG 1 cut(s) 741
MvaI CCWGG 4 cut(s) 222, 408, 441, 931
MwoI GCNNNNNNNGC 4 cut(s) 78, 478, 568, 577
NciI CCSGG 6 cut(s) 177, 263, 432, 606, 615, 645
NdeII GATC 4 cut(s) 350, 647, 808, 955
NlaIII CATG 8 cut(s) 310, 400, 421, 709, 740, 860, 905, 945
NlaIV GGNNCC 6 cut(s) 27, 226, 430, 499, 612, 613
NspI RCATGY 2 cut(s) 310, 945
PaeR7I CTCGAG 1 cut(s) 311
PagI TCATGA 2 cut(s) 736, 856
PcsI WCGNNNNNNNCGW 1 cut(s) 656
PfeI GAWTC 4 cut(s) 86, 589, 853, 1051
PfoI TCCNGGA 1 cut(s) 613
PkrI GCNGC 7 cut(s) 71, 241, 369, 570, 680, 870, 979
PpuMI RGGWCCY 1 cut(s) 611
Psp5II RGGWCCY 1 cut(s) 611
Psp6I CCWGG 4 cut(s) 220, 406, 439, 929
PspFI CCCAGC 2 cut(s) 81, 194
PspGI CCWGG 4 cut(s) 220, 406, 439, 929
PspN4I GGNNCC 6 cut(s) 27, 226, 430, 499, 612, 613
PspOMI GGGCCC 2 cut(s) 25, 497
PspPPI RGGWCCY 1 cut(s) 611
PspXI VCTCGAGB 1 cut(s) 311
PstI CTGCAG 1 cut(s) 493
PsuI RGATCY 1 cut(s) 955
RsaI GTAC 3 cut(s) 296, 515, 704
RsaNI GTAC 3 cut(s) 295, 514, 703
RseI CAYNNNNRTG 1 cut(s) 741
SaqAI TTAA 2 cut(s) 837, 936
SatI GCNGC 7 cut(s) 70, 240, 368, 569, 679, 869, 978
Sau3AI GATC 4 cut(s) 350, 647, 808, 955
SduI GDGCHC 4 cut(s) 29, 474, 501, 534
SexAI ACCWGGT 1 cut(s) 439
SfaNI GCATC 2 cut(s) 549, 805
SfcI CTRYAG 1 cut(s) 489
Sfr274I CTCGAG 1 cut(s) 311
SgrAI CRCCGGYG 1 cut(s) 289
SinI GGWCC 5 cut(s) 11, 179, 428, 437, 611
SlaI CTCGAG 1 cut(s) 311
SmiMI CAYNNNNRTG 1 cut(s) 741
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
Sse9I AATT 4 cut(s) 460, 551, 572, 813
SspMI CTAG 2 cut(s) 234, 467
StyI CCWWGG 2 cut(s) 21, 1039
TaaI ACNGT 1 cut(s) 341
TaqI TCGA 4 cut(s) 312, 349, 474, 650
TaqII GACCGA 1 cut(s) 416
TasI AATT 4 cut(s) 460, 551, 572, 813
TatI WGTACW 1 cut(s) 702
TauI GCSGC 3 cut(s) 72, 370, 681
TfiI GAWTC 4 cut(s) 86, 589, 853, 1051
Tru1I TTAA 2 cut(s) 837, 936
Tru9I TTAA 2 cut(s) 837, 936
TscAI CASTG 2 cut(s) 688, 920
TseI GCWGC 4 cut(s) 239, 568, 868, 977
TspDTI ATGAA 4 cut(s) 406, 413, 845, 1037
TspGWI ACGGA 1 cut(s) 648
TspRI CASTG 2 cut(s) 688, 920
VpaK11BI GGWCC 5 cut(s) 11, 179, 428, 437, 611
XagI CCTNNNNNAGG 1 cut(s) 492
XapI RAATTY 1 cut(s) 460
XceI RCATGY 2 cut(s) 310, 945
XhoI CTCGAG 1 cut(s) 311
XspI CTAG 2 cut(s) 234, 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.