RLG00000008005

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0008379
Physical Location & Seq
Forward (+)
24188909 .. 24198425
9517 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008005

Sequence Viewer

Length: 1536 bp
ATGGCTGGAGGTAACCACCCCAAGGCCCCACCACACAGCAACAAGCCTTCCTCTTCCTCCGCCCCTAACCGCAAATCCCGCTGGGAATCCTCCCCCTCCAGCAACATCAAAAACAACCACCCCCACCCACCCGACCCGACCAAACCCCCCAAGCCCACCACCACCGGCCCATCCCCCAATAAGGCCCCCACCGCCAGCCCCGCCAACCCCAAGCGCCCGCCCCTCCCCTCTCCCGGCGCCGCCCCTCCCTTTCCCTTCCCGGACCCCGCCGCGTTCGGCCCGCCGCCTCCGCCCGTCTACGGCTTCCACAACCTCGAGCGCCGCACGATAGTTCTCGCCGACGGTAGCGTCCGCTCCTACTTCGCCCTCCCGCCAGATTACCAAGACTTCCCTCCTCCTCCTCCTCCTCCTCCTCATATGGACCTGTCGGGGCGGTTCTTGCCGTTCGGGCCGGGCCGGCCGGCCGCTCCGGACTACTGGAATTCGCTTGGGCTGGACGGCCGTGGGCCCCAGGAAGGGTCGATGAAGAGGAAGTTTGGGGAAGAAGAGGAGCTGAGGGATAAGGGAGAAGAGTTGGCCAAGCGGCGGCAGCAGTTGGTGCAATTCGGAAACGGGTTTGCGGCGGGTCCGAGTAGCCCGTTTCGGCGTGACTTGGGGGCCCAATCCAGAAGTGGCGATGATCCGAGAGCTTCCAAGTATATGAGGACTGGTGGGGGTTATGAGAATGTGGGGTTTAGGCAAGGCGGCGGCGGCGGAGGTGGTGATAATGTGGGTCATAAGCATCTTCAGGTTGACCAGGCTGCGTTGAAGAAGGCATTTCTGTACTTTGCAAAGGTGATTAATGAGAATGGAGCGCAGAAGAAGATTTACATAGAGGATGGGAAGCAGGGACGGCTTAATTGTCTTGCTTGTGGCACCAGTGGCAGGTCTGCAAAAGATTTTCCGGATATGCATAGTCTCATCATGCACAGTTACAACATGGACAATGCGGACATTCGTGTGGATCACTTGGGCTTACACAAGGCTTTGTGTGTTTTAATGGGATGGAACTACTCAAAGCCTCCTGATAACTCAAAGGCATATCAGTTCCTTTCCGCTGATGAAGCAGCAGCAAATCAAGATGACTTGATTATGTGGCCACCCATGGTGATAATTCATAACACGCTTACAGGAAAGAGTAAAGATGGCCGCATGGAGGGTTTGGGCAATAAAGCTATGGATAGTTATATACGAGATCTTGGGTTTGGGAGTGGCAAGTCGAAGTCCCTGTACGGTAGAGATGGACATTTGGGAACAACTTTAATCAAGTTTTCAGCTGATGAAGCGGGCCTCAAGGAGGCCTTGCGAATGACAGAATTCTTTGAGAAGGACAAGCACGGACGCAGGGCTTGGTATCATGTACAACCGCTCACGTTGGGCAATAAGGATGATGAGAACAATCCTTATCTTATGAAGTTGGATGAAAAGACTCGGGAAAAGAAGAGAGTCCTTTATGAGTCCTGTGAGATCCAAGTGGTTTCATGGTGTGGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

512

Amino Acids

56.04

Weight (kDa)

9.28

Isoelectric Point (pI)

57.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 374 - 479 2.3e-23 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 347
Acc36I ACCTGC 1 cut(s) 915
AccB1I GGYRCC 2 cut(s) 236, 914
AccBSI CCGCTC 3 cut(s) 354, 467, 1408
AccI GTMKAC 1 cut(s) 297
AccII CGCG 1 cut(s) 272
AccIII TCCGGA 2 cut(s) 469, 943
AclWI GGATC 3 cut(s) 674, 1011, 1501
AcoI YGGCCR 6 cut(s) 458, 462, 499, 576, 1136, 1186
AcsI RAATTY 2 cut(s) 481, 1355
AcuI CTGAAG 1 cut(s) 770
AcyI GRCGYC 1 cut(s) 237
AfaI GTAC 3 cut(s) 824, 1271, 1401
AgsI TTSAA 1 cut(s) 808
AjnI CCWGG 2 cut(s) 510, 795
AluBI AGCT 4 cut(s) 553, 689, 1214, 1316
AluI AGCT 4 cut(s) 553, 689, 1214, 1316
Alw26I GTCTC 1 cut(s) 962
AlwI GGATC 3 cut(s) 674, 1011, 1501
Ama87I CYCGRG 2 cut(s) 314, 1470
Aor13HI TCCGGA 2 cut(s) 469, 943
ApaI GGGCCC 2 cut(s) 510, 661
ApeKI GCWGC 4 cut(s) 589, 800, 1106, 1109
ApoI RAATTY 2 cut(s) 481, 1355
AseI ATTAAT 1 cut(s) 840
AspLEI GCGC 4 cut(s) 216, 239, 321, 856
AsuC2I CCSGG 3 cut(s) 234, 260, 453
AsuHPI GGTGA 3 cut(s) 773, 847, 1159
AvaI CYCGRG 2 cut(s) 314, 1470
AvaII GGWCC 3 cut(s) 262, 421, 626
BaeGI GKGCMC 2 cut(s) 510, 661
BalI TGGCCA 2 cut(s) 578, 1138
BanI GGYRCC 2 cut(s) 236, 914
BanII GRGCYC 2 cut(s) 510, 661
BarI GAAGNNNNNNTAC 4 cut(s) 851, 883, 1253, 1285
BbvCI CCTCAGC 1 cut(s) 554
BbvI GCAGC 4 cut(s) 601, 787, 1118, 1121
BccI CCATC 5 cut(s) 178, 872, 1038, 1178, 1274
BceAI ACGGC 5 cut(s) 316, 427, 486, 514, 908
BciT130I CCWGG 2 cut(s) 512, 797
BcnI CCSGG 3 cut(s) 234, 260, 453
BcoDI GTCTC 1 cut(s) 962
BfoI RGCGCY 3 cut(s) 217, 240, 322
BfuAI ACCTGC 1 cut(s) 915
BglI GCCNNNNNGGC 2 cut(s) 448, 457
BglII AGATCT 1 cut(s) 1234
Bme1390I CCNGG 5 cut(s) 234, 260, 453, 512, 797
Bme18I GGWCC 3 cut(s) 262, 421, 626
BmeT110I CYCGRG 2 cut(s) 314, 1470
BmrFI CCNGG 5 cut(s) 234, 260, 453, 512, 797
BmsI GCATC 1 cut(s) 790
BpmI CTGGAG 2 cut(s) 27, 82
Bpu10I CCTNAGC 1 cut(s) 554
BpuEI CTTGAG 1 cut(s) 1316
BpuMI CCSGG 3 cut(s) 234, 260, 453
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 4 cut(s) 21, 502, 510, 1143
BsaWI WCCGGW 2 cut(s) 469, 943
Bse118I RCCGGY 3 cut(s) 164, 456, 460
Bse1I ACTGG 3 cut(s) 482, 712, 918
BseAI TCCGGA 2 cut(s) 469, 943
BseBI CCWGG 2 cut(s) 512, 797
BseDI CCNNGG 4 cut(s) 21, 502, 510, 1143
BseGI GGATG 5 cut(s) 170, 883, 1049, 1432, 1465
BseMII CTCAG 1 cut(s) 545
BseNI ACTGG 3 cut(s) 482, 712, 918
BseRI GAGGAG 8 cut(s) 384, 387, 390, 393, 396, 399, 402, 563
BseSI GKGCMC 2 cut(s) 510, 661
BseX3I CGGCCG 3 cut(s) 458, 462, 499
BseXI GCAGC 4 cut(s) 601, 787, 1118, 1121
BseYI CCCAGC 1 cut(s) 81
Bsh1236I CGCG 1 cut(s) 272
Bsh1285I CGRYCG 3 cut(s) 461, 465, 502
BshNI GGYRCC 2 cut(s) 236, 914
BsiEI CGRYCG 3 cut(s) 461, 465, 502
BsiHKCI CYCGRG 2 cut(s) 314, 1470
BsiSI CCGG 8 cut(s) 165, 234, 260, 452, 457, 461, 470, 944
BslFI GGGAC 2 cut(s) 903, 1249
BsmAI GTCTC 1 cut(s) 962
BsmFI GGGAC 2 cut(s) 903, 1249
BsoBI CYCGRG 2 cut(s) 314, 1470
Bsp120I GGGCCC 2 cut(s) 506, 657
Bsp1286I GDGCHC 2 cut(s) 510, 661
Bsp13I TCCGGA 2 cut(s) 469, 943
Bsp1407I TGTACA 1 cut(s) 1399
Bsp143I GATC 4 cut(s) 679, 1003, 1234, 1506
Bsp19I CCATGG 1 cut(s) 1143
BspCNI CTCAG 1 cut(s) 546
BspEI TCCGGA 2 cut(s) 469, 943
BspFNI CGCG 1 cut(s) 272
BspMI ACCTGC 1 cut(s) 915
BspPI GGATC 3 cut(s) 674, 1011, 1501
BspT107I GGYRCC 2 cut(s) 236, 914
BsrBI CCGCTC 3 cut(s) 354, 467, 1408
BsrFI RCCGGY 3 cut(s) 164, 456, 460
BsrGI TGTACA 1 cut(s) 1399
BsrI ACTGG 3 cut(s) 482, 712, 918
BssAI RCCGGY 3 cut(s) 164, 456, 460
BssECI CCNNGG 4 cut(s) 21, 502, 510, 1143
BssMI GATC 4 cut(s) 679, 1003, 1234, 1506
BssNI GRCGYC 1 cut(s) 237
BssT1I CCWWGG 2 cut(s) 21, 1143
Bst2UI CCWGG 2 cut(s) 512, 797
Bst4CI ACNGT 3 cut(s) 344, 971, 1274
Bst6I CTCTTC 5 cut(s) 58, 521, 540, 564, 1475
BstACI GRCGYC 1 cut(s) 237
BstAPI GCANNNNNTGC 1 cut(s) 598
BstAUI TGTACA 1 cut(s) 1399
BstC8I GCNNGC 6 cut(s) 196, 218, 281, 458, 462, 1327
BstDEI CTNAG 1 cut(s) 554
BstDSI CCRYGG 2 cut(s) 502, 1143
BstEII GGTNACC 1 cut(s) 11
BstENI CCTNNNNNAGG 1 cut(s) 1334
BstF5I GGATG 5 cut(s) 170, 883, 1049, 1432, 1465
BstFNI CGCG 1 cut(s) 272
BstH2I RGCGCY 3 cut(s) 217, 240, 322
BstHHI GCGC 4 cut(s) 216, 239, 321, 856
BstKTI GATC 4 cut(s) 682, 1006, 1237, 1509
BstMAI GTCTC 1 cut(s) 962
BstMBI GATC 4 cut(s) 679, 1003, 1234, 1506
BstMCI CGRYCG 3 cut(s) 461, 465, 502
BstNI CCWGG 2 cut(s) 512, 797
BstPI GGTNACC 1 cut(s) 11
BstSCI CCNGG 5 cut(s) 232, 258, 451, 510, 795
BstSLI GKGCMC 2 cut(s) 510, 661
BstUI CGCG 1 cut(s) 272
BstV1I GCAGC 4 cut(s) 601, 787, 1118, 1121
BstX2I RGATCY 2 cut(s) 1234, 1506
BstYI RGATCY 2 cut(s) 1234, 1506
BstZI CGGCCG 3 cut(s) 458, 462, 499
BtgI CCRYGG 2 cut(s) 502, 1143
BtgZI GCGATG 1 cut(s) 690
BtsCI GGATG 5 cut(s) 170, 883, 1049, 1432, 1465
BtsIMutI CAGTG 1 cut(s) 925
BveI ACCTGC 1 cut(s) 915
Cac8I GCNNGC 6 cut(s) 196, 218, 281, 458, 462, 1327
CfoI GCGC 4 cut(s) 216, 239, 321, 856
Cfr10I RCCGGY 3 cut(s) 164, 456, 460
CseI GACGC 2 cut(s) 337, 1389
Csp6I GTAC 3 cut(s) 823, 1270, 1400
CviAII CATG 6 cut(s) 964, 979, 1144, 1192, 1397, 1521
CviQI GTAC 3 cut(s) 823, 1270, 1400
DdeI CTNAG 1 cut(s) 554
DinI GGCGCC 1 cut(s) 238
DpnI GATC 4 cut(s) 681, 1005, 1236, 1508
DpnII GATC 4 cut(s) 679, 1003, 1234, 1506
DrdI GACNNNNNNGTC 1 cut(s) 347
DseDI GACNNNNNNGTC 1 cut(s) 347
EaeI YGGCCR 6 cut(s) 458, 462, 499, 576, 1136, 1186
EagI CGGCCG 3 cut(s) 458, 462, 499
Eam1104I CTCTTC 5 cut(s) 58, 521, 540, 564, 1475
EarI CTCTTC 5 cut(s) 58, 521, 540, 564, 1475
EciI GGCGGA 3 cut(s) 49, 279, 768
EclXI CGGCCG 3 cut(s) 458, 462, 499
Eco130I CCWWGG 2 cut(s) 21, 1143
Eco147I AGGCCT 1 cut(s) 1340
Eco24I GRGCYC 2 cut(s) 510, 661
Eco47I GGWCC 3 cut(s) 262, 421, 626
Eco52I CGGCCG 3 cut(s) 458, 462, 499
Eco57I CTGAAG 1 cut(s) 770
Eco88I CYCGRG 2 cut(s) 314, 1470
Eco91I GGTNACC 1 cut(s) 11
EcoNI CCTNNNNNAGG 1 cut(s) 1334
EcoO109I RGGNCCY 4 cut(s) 25, 184, 507, 657
EcoO65I GGTNACC 1 cut(s) 11
EcoRI GAATTC 2 cut(s) 481, 1355
EcoRII CCWGG 2 cut(s) 510, 795
EcoT14I CCWWGG 2 cut(s) 21, 1143
EcoT22I ATGCAT 1 cut(s) 954
EcoT38I GRGCYC 2 cut(s) 510, 661
EgeI GGCGCC 1 cut(s) 238
EheI GGCGCC 1 cut(s) 238
ErhI CCWWGG 2 cut(s) 21, 1143
FaeI CATG 6 cut(s) 967, 982, 1147, 1195, 1400, 1524
FalI AAGNNNNNCTT 2 cut(s) 1325, 1357
FaqI GGGAC 2 cut(s) 903, 1249
FatI CATG 6 cut(s) 963, 978, 1143, 1191, 1396, 1520
FauI CCCGC 8 cut(s) 86, 208, 225, 274, 288, 378, 616, 1318
FauNDI CATATG 1 cut(s) 417
FblI GTMKAC 1 cut(s) 297
FokI GGATG 5 cut(s) 157, 890, 1056, 1439, 1472
FriOI GRGCYC 2 cut(s) 510, 661
FseI GGCCGGCC 2 cut(s) 460, 464
GlaI GCGC 4 cut(s) 215, 238, 320, 855
GsaI CCCAGC 1 cut(s) 85
GsuI CTGGAG 2 cut(s) 27, 82
HaeII RGCGCY 3 cut(s) 217, 240, 322
HapII CCGG 8 cut(s) 165, 234, 260, 452, 457, 461, 470, 944
HgaI GACGC 2 cut(s) 337, 1389
HhaI GCGC 4 cut(s) 216, 239, 321, 856
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 6 cut(s) 967, 982, 1147, 1195, 1400, 1524
Hin6I GCGC 4 cut(s) 214, 237, 319, 854
HinP1I GCGC 4 cut(s) 214, 237, 319, 854
HincII GTYRAC 1 cut(s) 793
HindII GTYRAC 1 cut(s) 793
HinfI GANTC 4 cut(s) 86, 1468, 1485, 1496
HpaII CCGG 8 cut(s) 165, 234, 260, 452, 457, 461, 470, 944
HphI GGTGA 3 cut(s) 773, 847, 1159
Hpy166II GTNNAC 2 cut(s) 298, 793
Hpy188I TCNGA 3 cut(s) 608, 630, 684
Hpy188III TCNNGA 6 cut(s) 470, 666, 944, 1064, 1118, 1472
Hpy8I GTNNAC 2 cut(s) 298, 793
Hpy99I CGWCG 1 cut(s) 344
HpyAV CCTTC 6 cut(s) 57, 265, 509, 805, 1360, 1524
HpyCH4III ACNGT 3 cut(s) 344, 971, 1274
HpyCH4IV ACGT 1 cut(s) 1412
HpyCH4V TGCA 5 cut(s) 601, 830, 932, 952, 967
HpyF3I CTNAG 1 cut(s) 554
HpySE526I ACGT 1 cut(s) 1412
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 6 cut(s) 967, 982, 1147, 1195, 1400, 1524
HspAI GCGC 4 cut(s) 214, 237, 319, 854
KasI GGCGCC 1 cut(s) 236
Kpn2I TCCGGA 2 cut(s) 469, 943
KroI GCCGGC 2 cut(s) 456, 460
KroNI GCCGGC 2 cut(s) 458, 462
Kzo9I GATC 4 cut(s) 679, 1003, 1234, 1506
LmnI GCTCC 4 cut(s) 359, 472, 550, 851
Lsp1109I GCAGC 4 cut(s) 601, 787, 1118, 1121
LweI GCATC 1 cut(s) 790
MaeII ACGT 1 cut(s) 1412
MaeIII GTNAC 3 cut(s) 11, 647, 971
MalI GATC 4 cut(s) 681, 1005, 1236, 1508
MbiI CCGCTC 3 cut(s) 354, 467, 1408
MboI GATC 4 cut(s) 679, 1003, 1234, 1506
MflI RGATCY 2 cut(s) 1234, 1506
MhlI GDGCHC 2 cut(s) 510, 661
MlsI TGGCCA 2 cut(s) 578, 1138
MluCI AATT 5 cut(s) 481, 602, 898, 1152, 1355
MluNI TGGCCA 2 cut(s) 578, 1138
Mly113I GGCGCC 1 cut(s) 237
MlyI GAGTC 3 cut(s) 1462, 1494, 1505
MmeI TCCRAC 1 cut(s) 1437
Mox20I TGGCCA 2 cut(s) 578, 1138
Mph1103I ATGCAT 1 cut(s) 954
MroI TCCGGA 2 cut(s) 469, 943
MroNI GCCGGC 2 cut(s) 456, 460
MscI TGGCCA 2 cut(s) 578, 1138
MseI TTAA 4 cut(s) 840, 897, 1037, 1301
MslI CAYNNNNRTG 1 cut(s) 998
Msp20I TGGCCA 2 cut(s) 578, 1138
MspA1I CMGCKG 3 cut(s) 81, 1097, 1316
MspI CCGG 8 cut(s) 165, 234, 260, 452, 457, 461, 470, 944
MspR9I CCNGG 5 cut(s) 234, 260, 453, 512, 797
MvaI CCWGG 2 cut(s) 512, 797
MvnI CGCG 1 cut(s) 272
NaeI GCCGGC 2 cut(s) 458, 462
NarI GGCGCC 1 cut(s) 237
NciI CCSGG 3 cut(s) 234, 260, 453
NcoI CCATGG 1 cut(s) 1143
NdeI CATATG 1 cut(s) 417
NdeII GATC 4 cut(s) 679, 1003, 1234, 1506
NgoMIV GCCGGC 2 cut(s) 456, 460
NlaIII CATG 6 cut(s) 967, 982, 1147, 1195, 1400, 1524
NmuCI GTSAC 1 cut(s) 647
NsiI ATGCAT 1 cut(s) 954
PaeR7I CTCGAG 1 cut(s) 314
PceI AGGCCT 1 cut(s) 1340
PdiI GCCGGC 2 cut(s) 458, 462
PfeI GAWTC 1 cut(s) 86
PfoI TCCNGGA 1 cut(s) 258
PleI GAGTC 3 cut(s) 1462, 1493, 1504
PluTI GGCGCC 1 cut(s) 240
PpsI GAGTC 3 cut(s) 1462, 1493, 1504
PshBI ATTAAT 1 cut(s) 840
Psp6I CCWGG 2 cut(s) 510, 795
PspEI GGTNACC 1 cut(s) 11
PspFI CCCAGC 1 cut(s) 81
PspGI CCWGG 2 cut(s) 510, 795
PspOMI GGGCCC 2 cut(s) 506, 657
PspXI VCTCGAGB 1 cut(s) 314
PsuI RGATCY 2 cut(s) 1234, 1506
PvuII CAGCTG 1 cut(s) 1316
RigI GGCCGGCC 2 cut(s) 460, 464
RsaI GTAC 3 cut(s) 824, 1271, 1401
RsaNI GTAC 3 cut(s) 823, 1270, 1400
RseI CAYNNNNRTG 1 cut(s) 998
SaqAI TTAA 4 cut(s) 840, 897, 1037, 1301
Sau3AI GATC 4 cut(s) 679, 1003, 1234, 1506
SchI GAGTC 3 cut(s) 1462, 1494, 1505
ScrFI CCNGG 5 cut(s) 234, 260, 453, 512, 797
SduI GDGCHC 2 cut(s) 510, 661
SfaNI GCATC 1 cut(s) 790
SfiI GGCCNNNNNGGCC 1 cut(s) 457
SfoI GGCGCC 1 cut(s) 238
Sfr274I CTCGAG 1 cut(s) 314
SinI GGWCC 3 cut(s) 262, 421, 626
SlaI CTCGAG 1 cut(s) 314
SmiMI CAYNNNNRTG 1 cut(s) 998
SmlI CTYRAG 2 cut(s) 314, 1331
SmoI CTYRAG 2 cut(s) 314, 1331
Sse9I AATT 5 cut(s) 481, 602, 898, 1152, 1355
SseBI AGGCCT 1 cut(s) 1340
SspDI GGCGCC 1 cut(s) 236
StuI AGGCCT 1 cut(s) 1340
StyD4I CCNGG 5 cut(s) 232, 258, 451, 510, 795
StyI CCWWGG 2 cut(s) 21, 1143
TaaI ACNGT 3 cut(s) 344, 971, 1274
TaiI ACGT 1 cut(s) 1415
TaqI TCGA 3 cut(s) 315, 521, 1259
TasI AATT 5 cut(s) 481, 602, 898, 1152, 1355
TatI WGTACW 2 cut(s) 822, 1399
TfiI GAWTC 1 cut(s) 86
Tru1I TTAA 4 cut(s) 840, 897, 1037, 1301
Tru9I TTAA 4 cut(s) 840, 897, 1037, 1301
TscAI CASTG 1 cut(s) 925
TseFI GTSAC 1 cut(s) 647
TseI GCWGC 4 cut(s) 589, 800, 1106, 1109
Tsp45I GTSAC 1 cut(s) 647
TspDTI ATGAA 7 cut(s) 539, 1116, 1145, 1335, 1466, 1476, 1509
TspGWI ACGGA 1 cut(s) 1392
TspRI CASTG 1 cut(s) 925
VpaK11BI GGWCC 3 cut(s) 262, 421, 626
VspI ATTAAT 1 cut(s) 840
XagI CCTNNNNNAGG 1 cut(s) 1334
XapI RAATTY 2 cut(s) 481, 1355
XcmI CCANNNNNNNNNTGG 1 cut(s) 668
XhoI CTCGAG 1 cut(s) 314
XmiI GTMKAC 1 cut(s) 297
Zsp2I ATGCAT 1 cut(s) 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.