Rmu_sc0032525.1_g000002

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0032525.1
OG0008379
Physical Location & Seq
Forward (+)
651 .. 1250
600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0032525.1_g000002.1.cds

Sequence Viewer

Length: 600 bp
atggacccgtcggggcggttcttgccgttcgggccgggccggccggccgccccggactactggaattcgcttgggctggacggccgtgggccccaggaagggtcgatgaagaggaagtttggggaagaagaggagctgagggataagggagaagagttggccaagcggcggcagcagttggtgcaattcggaaacgggtttggggcgggtccgagtagcccgtttcggcgtgacttgggggcccaatccagaagtggcgatgatccgagggcttccaagtatatgaggactggtgggggttatgagaatgtggggtttaggcaaggcggtggcggcggaggtggtgataatgtgggtcataagcatcttcaggttgaccaggctgcgttgaagaaggcatttctgtactttgcgaaggtgattaatgagaatggagcgcagaagaagatttacatagaggatgggaagcagggacggcttaattgtcttgcttgtggcaccactggcaggtctgatactgatcgtttcgctctctttaaatttaaatttacaggatgtttattttcgatgctaatattatccatgggttggttgatttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

199

Amino Acids

21.74

Weight (kDa)

9.51

Isoelectric Point (pI)

54.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 498
AccB1I GGYRCC 1 cut(s) 497
AccB7I CCANNNNNTGG 1 cut(s) 588
AciI CCGC 8 cut(s) 16, 48, 166, 169, 206, 327, 333, 336
AclWI GGATC 1 cut(s) 257
AcoI YGGCCR 4 cut(s) 41, 45, 82, 159
AcsI RAATTY 3 cut(s) 64, 539, 545
AcuI CTGAAG 1 cut(s) 353
AfaI GTAC 1 cut(s) 407
AfiI CCNNNNNNNGG 5 cut(s) 98, 99, 168, 507, 588
AgsI TTSAA 1 cut(s) 391
AjnI CCWGG 2 cut(s) 93, 378
AluBI AGCT 1 cut(s) 136
AluI AGCT 1 cut(s) 136
AlwI GGATC 1 cut(s) 257
AoxI GGCC 8 cut(s) 32, 37, 41, 45, 82, 89, 159, 240
ApaI GGGCCC 2 cut(s) 93, 244
ApeKI GCWGC 2 cut(s) 172, 383
ApoI RAATTY 3 cut(s) 64, 539, 545
AseI ATTAAT 1 cut(s) 423
AspLEI GCGC 1 cut(s) 439
AspS9I GGNCC 8 cut(s) 4, 32, 37, 89, 90, 209, 240, 241
AsuC2I CCSGG 2 cut(s) 36, 53
AsuHPI GGTGA 2 cut(s) 356, 430
AvaII GGWCC 2 cut(s) 4, 209
BaeGI GKGCMC 2 cut(s) 93, 244
BalI TGGCCA 1 cut(s) 161
BanI GGYRCC 1 cut(s) 497
BanII GRGCYC 2 cut(s) 93, 244
BarI GAAGNNNNNNTAC 2 cut(s) 434, 466
BbvCI CCTCAGC 1 cut(s) 137
BbvI GCAGC 2 cut(s) 184, 370
BccI CCATC 1 cut(s) 455
BceAI ACGGC 4 cut(s) 10, 69, 97, 491
BciT130I CCWGG 2 cut(s) 95, 380
BcnI CCSGG 2 cut(s) 36, 53
BfuAI ACCTGC 1 cut(s) 498
BglI GCCNNNNNGGC 2 cut(s) 31, 40
BisI GCNGC 6 cut(s) 48, 167, 170, 173, 334, 384
BlsI GCNGC 6 cut(s) 49, 168, 171, 174, 335, 385
Bme1390I CCNGG 4 cut(s) 36, 53, 95, 380
Bme18I GGWCC 2 cut(s) 4, 209
BmgT120I GGNCC 8 cut(s) 4, 32, 37, 89, 90, 209, 240, 241
BmiI GGNNCC 7 cut(s) 6, 91, 92, 210, 241, 242, 499
BmrFI CCNGG 4 cut(s) 36, 53, 95, 380
BmsI GCATC 2 cut(s) 373, 558
Bpu10I CCTNAGC 1 cut(s) 137
BpuMI CCSGG 2 cut(s) 36, 53
BsaBI GATNNNNATC 1 cut(s) 519
BsaJI CCNNGG 5 cut(s) 51, 85, 93, 266, 582
Bsc4I CCNNNNNNNGG 5 cut(s) 98, 99, 168, 507, 588
Bse118I RCCGGY 2 cut(s) 39, 43
Bse1I ACTGG 3 cut(s) 65, 295, 508
Bse8I GATNNNNATC 1 cut(s) 519
BseBI CCWGG 2 cut(s) 95, 380
BseDI CCNNGG 5 cut(s) 51, 85, 93, 266, 582
BseGI GGATG 2 cut(s) 466, 560
BseJI GATNNNNATC 1 cut(s) 519
BseLI CCNNNNNNNGG 5 cut(s) 98, 99, 168, 507, 588
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 3 cut(s) 65, 295, 508
BseRI GAGGAG 1 cut(s) 146
BseSI GKGCMC 2 cut(s) 93, 244
BseX3I CGGCCG 3 cut(s) 41, 45, 82
BseXI GCAGC 2 cut(s) 184, 370
Bsh1285I CGRYCG 3 cut(s) 44, 48, 85
BshFI GGCC 8 cut(s) 34, 39, 43, 47, 84, 91, 161, 242
BshNI GGYRCC 1 cut(s) 497
BsiEI CGRYCG 3 cut(s) 44, 48, 85
BsiSI CCGG 4 cut(s) 35, 40, 44, 53
BslFI GGGAC 1 cut(s) 486
BslI CCNNNNNNNGG 5 cut(s) 98, 99, 168, 507, 588
BsmFI GGGAC 1 cut(s) 486
BsnI GGCC 8 cut(s) 34, 39, 43, 47, 84, 91, 161, 242
Bsp120I GGGCCC 2 cut(s) 89, 240
Bsp1286I GDGCHC 2 cut(s) 93, 244
Bsp143I GATC 2 cut(s) 262, 520
Bsp19I CCATGG 1 cut(s) 582
BspACI CCGC 8 cut(s) 16, 48, 166, 169, 206, 327, 333, 336
BspANI GGCC 8 cut(s) 34, 39, 43, 47, 84, 91, 161, 242
BspCNI CTCAG 1 cut(s) 129
BspLI GGNNCC 7 cut(s) 6, 91, 92, 210, 241, 242, 499
BspMI ACCTGC 1 cut(s) 498
BspPI GGATC 1 cut(s) 257
BspT107I GGYRCC 1 cut(s) 497
BsrFI RCCGGY 2 cut(s) 39, 43
BsrI ACTGG 3 cut(s) 65, 295, 508
BssAI RCCGGY 2 cut(s) 39, 43
BssECI CCNNGG 5 cut(s) 51, 85, 93, 266, 582
BssMI GATC 2 cut(s) 262, 520
BssT1I CCWWGG 1 cut(s) 582
Bst2UI CCWGG 2 cut(s) 95, 380
Bst6I CTCTTC 3 cut(s) 104, 123, 147
BstAPI GCANNNNNTGC 1 cut(s) 181
BstC8I GCNNGC 2 cut(s) 41, 45
BstDEI CTNAG 1 cut(s) 137
BstDSI CCRYGG 2 cut(s) 85, 582
BstF5I GGATG 2 cut(s) 466, 560
BstHHI GCGC 1 cut(s) 439
BstKTI GATC 2 cut(s) 265, 523
BstMBI GATC 2 cut(s) 262, 520
BstMCI CGRYCG 3 cut(s) 44, 48, 85
BstMWI GCNNNNNNNGC 8 cut(s) 22, 31, 40, 172, 181, 333, 475, 504
BstNI CCWGG 2 cut(s) 95, 380
BstSCI CCNGG 4 cut(s) 34, 51, 93, 378
BstSLI GKGCMC 2 cut(s) 93, 244
BstV1I GCAGC 2 cut(s) 184, 370
BstZI CGGCCG 3 cut(s) 41, 45, 82
BsuRI GGCC 8 cut(s) 34, 39, 43, 47, 84, 91, 161, 242
BtgI CCRYGG 2 cut(s) 85, 582
BtgZI GCGATG 1 cut(s) 273
BtsCI GGATG 2 cut(s) 466, 560
BtsIMutI CAGTG 1 cut(s) 501
BveI ACCTGC 1 cut(s) 498
Cac8I GCNNGC 2 cut(s) 41, 45
CfoI GCGC 1 cut(s) 439
Cfr10I RCCGGY 2 cut(s) 39, 43
Cfr13I GGNCC 8 cut(s) 4, 32, 37, 89, 90, 209, 240, 241
Csp6I GTAC 1 cut(s) 406
CviAII CATG 1 cut(s) 583
CviQI GTAC 1 cut(s) 406
DdeI CTNAG 1 cut(s) 137
DpnI GATC 2 cut(s) 264, 522
DpnII GATC 2 cut(s) 262, 520
DraI TTTAAA 2 cut(s) 538, 544
EaeI YGGCCR 4 cut(s) 41, 45, 82, 159
EagI CGGCCG 3 cut(s) 41, 45, 82
Eam1104I CTCTTC 3 cut(s) 104, 123, 147
EarI CTCTTC 3 cut(s) 104, 123, 147
EciI GGCGGA 1 cut(s) 351
EclXI CGGCCG 3 cut(s) 41, 45, 82
Eco130I CCWWGG 1 cut(s) 582
Eco24I GRGCYC 2 cut(s) 93, 244
Eco47I GGWCC 2 cut(s) 4, 209
Eco52I CGGCCG 3 cut(s) 41, 45, 82
Eco57I CTGAAG 1 cut(s) 353
EcoO109I RGGNCCY 2 cut(s) 90, 240
EcoRI GAATTC 1 cut(s) 64
EcoRII CCWGG 2 cut(s) 93, 378
EcoT14I CCWWGG 1 cut(s) 582
EcoT38I GRGCYC 2 cut(s) 93, 244
ErhI CCWWGG 1 cut(s) 582
FaeI CATG 1 cut(s) 586
FaiI YATR 6 cut(s) 282, 284, 303, 360, 455, 584
FaqI GGGAC 1 cut(s) 486
FatI CATG 1 cut(s) 582
FauI CCCGC 1 cut(s) 199
Fnu4HI GCNGC 6 cut(s) 48, 167, 170, 173, 334, 384
FokI GGATG 2 cut(s) 473, 567
FriOI GRGCYC 2 cut(s) 93, 244
FseI GGCCGGCC 2 cut(s) 43, 47
Fsp4HI GCNGC 6 cut(s) 48, 167, 170, 173, 334, 384
GlaI GCGC 1 cut(s) 438
GluI GCNGC 6 cut(s) 48, 167, 170, 173, 334, 384
HaeIII GGCC 8 cut(s) 34, 39, 43, 47, 84, 91, 161, 242
HapII CCGG 4 cut(s) 35, 40, 44, 53
HhaI GCGC 1 cut(s) 439
Hin1II CATG 1 cut(s) 586
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HpaII CCGG 4 cut(s) 35, 40, 44, 53
HphI GGTGA 2 cut(s) 356, 430
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 4 cut(s) 191, 213, 267, 514
Hpy188III TCNNGA 1 cut(s) 249
Hpy8I GTNNAC 1 cut(s) 376
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 3 cut(s) 92, 388, 409
HpyCH4V TGCA 1 cut(s) 184
HpyF10VI GCNNNNNNNGC 8 cut(s) 22, 31, 40, 172, 181, 333, 475, 504
HpyF3I CTNAG 1 cut(s) 137
Hsp92II CATG 1 cut(s) 586
HspAI GCGC 1 cut(s) 437
KroI GCCGGC 2 cut(s) 39, 43
KroNI GCCGGC 2 cut(s) 41, 45
Kzo9I GATC 2 cut(s) 262, 520
LmnI GCTCC 2 cut(s) 133, 434
Lsp1109I GCAGC 2 cut(s) 184, 370
LweI GCATC 2 cut(s) 373, 558
MaeIII GTNAC 1 cut(s) 230
MalI GATC 2 cut(s) 264, 522
MboI GATC 2 cut(s) 262, 520
MboII GAAGA 8 cut(s) 121, 137, 140, 164, 359, 403, 454, 457
MhlI GDGCHC 2 cut(s) 93, 244
MlsI TGGCCA 1 cut(s) 161
MluCI AATT 5 cut(s) 64, 185, 481, 539, 545
MluNI TGGCCA 1 cut(s) 161
MnlI CCTC 7 cut(s) 105, 124, 132, 261, 279, 332, 451
Mox20I TGGCCA 1 cut(s) 161
MroNI GCCGGC 2 cut(s) 39, 43
MscI TGGCCA 1 cut(s) 161
MseI TTAA 4 cut(s) 423, 480, 537, 543
Msp20I TGGCCA 1 cut(s) 161
MspI CCGG 4 cut(s) 35, 40, 44, 53
MspR9I CCNGG 4 cut(s) 36, 53, 95, 380
MvaI CCWGG 2 cut(s) 95, 380
MwoI GCNNNNNNNGC 8 cut(s) 22, 31, 40, 172, 181, 333, 475, 504
NaeI GCCGGC 2 cut(s) 41, 45
NciI CCSGG 2 cut(s) 36, 53
NcoI CCATGG 1 cut(s) 582
NdeII GATC 2 cut(s) 262, 520
NgoMIV GCCGGC 2 cut(s) 39, 43
NlaIII CATG 1 cut(s) 586
NlaIV GGNNCC 7 cut(s) 6, 91, 92, 210, 241, 242, 499
NmuCI GTSAC 1 cut(s) 230
PdiI GCCGGC 2 cut(s) 41, 45
PflMI CCANNNNNTGG 1 cut(s) 588
PkrI GCNGC 6 cut(s) 49, 168, 171, 174, 335, 385
PshBI ATTAAT 1 cut(s) 423
Psp6I CCWGG 2 cut(s) 93, 378
PspGI CCWGG 2 cut(s) 93, 378
PspN4I GGNNCC 7 cut(s) 6, 91, 92, 210, 241, 242, 499
PspOMI GGGCCC 2 cut(s) 89, 240
PspPI GGNCC 8 cut(s) 4, 32, 37, 89, 90, 209, 240, 241
RigI GGCCGGCC 2 cut(s) 43, 47
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
SaqAI TTAA 4 cut(s) 423, 480, 537, 543
SatI GCNGC 6 cut(s) 48, 167, 170, 173, 334, 384
Sau3AI GATC 2 cut(s) 262, 520
Sau96I GGNCC 8 cut(s) 4, 32, 37, 89, 90, 209, 240, 241
ScrFI CCNGG 4 cut(s) 36, 53, 95, 380
SduI GDGCHC 2 cut(s) 93, 244
SetI ASST 5 cut(s) 138, 343, 375, 420, 512
SfaNI GCATC 2 cut(s) 373, 558
SfiI GGCCNNNNNGGCC 1 cut(s) 40
SinI GGWCC 2 cut(s) 4, 209
SmiI ATTTAAAT 1 cut(s) 544
Sse9I AATT 5 cut(s) 64, 185, 481, 539, 545
SsiI CCGC 8 cut(s) 16, 48, 166, 169, 206, 327, 333, 336
SspI AATATT 1 cut(s) 576
StyD4I CCNGG 4 cut(s) 34, 51, 93, 378
StyI CCWWGG 1 cut(s) 582
SwaI ATTTAAAT 1 cut(s) 544
TaqI TCGA 2 cut(s) 104, 566
TasI AATT 5 cut(s) 64, 185, 481, 539, 545
TatI WGTACW 1 cut(s) 405
TauI GCSGC 4 cut(s) 50, 169, 172, 336
Tru1I TTAA 4 cut(s) 423, 480, 537, 543
Tru9I TTAA 4 cut(s) 423, 480, 537, 543
TscAI CASTG 1 cut(s) 508
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 2 cut(s) 172, 383
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 1 cut(s) 122
TspRI CASTG 1 cut(s) 508
Van91I CCANNNNNTGG 1 cut(s) 588
VpaK11BI GGWCC 2 cut(s) 4, 209
VspI ATTAAT 1 cut(s) 423
XapI RAATTY 3 cut(s) 64, 539, 545
XcmI CCANNNNNNNNNTGG 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.