MD13G1221200.v1.1

Mitochondrial import receptor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005799
Physical Location & Seq
Forward (+)
21402511 .. 21402813
303 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1221200.v1.1.491

Sequence Viewer

Length: 303 bp
ATGGCGTCAGAGGGGAGAAAGCGACTGTCGCTCCGAAGCAACGGCGAAGAACAGAGCATGCTTTCGAGGGTGTCGCAATCGGAGATCGTGCAGCAGACGAAGCGCGTGGCGATCGACGCGTCGTCGGTGACGAAGAAGCTGGCGAAGAGCACGGGGAAGGCGGCGTGGATATTGGGAACGACGTTCCTGATCTTGGGGGTGCCACTCATCATCGCCATGGATCGTGAGCAGCAGTTCAACGAGTTGGAGTTGCAGCAGGCCAGCATCCTCGGTTCTCCTCCCCCTCCTCCCGCCTTCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tom22

Protein Analysis

101

Amino Acids

10.89

Weight (kDa)

9.69

Isoelectric Point (pI)

74.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tom22 PF04281 31 - 86 1.7e-10 Mitochondrial import receptor subunit Tom22
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 199
AccII CGCG 2 cut(s) 105, 119
AciI CCGC 2 cut(s) 161, 291
AclWI GGATC 1 cut(s) 228
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 193
AflIII ACRYGT 1 cut(s) 117
AgsI TTSAA 2 cut(s) 238, 298
AhdI GACNNNNNGTC 1 cut(s) 121
AluBI AGCT 1 cut(s) 139
AluI AGCT 1 cut(s) 139
Alw21I GWGCWC 1 cut(s) 152
AlwI GGATC 1 cut(s) 228
AoxI GGCC 1 cut(s) 258
ApeKI GCWGC 3 cut(s) 91, 229, 253
AspLEI GCGC 1 cut(s) 105
AsuHPI GGTGA 1 cut(s) 139
BanI GGYRCC 1 cut(s) 199
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 3 cut(s) 103, 241, 265
BceAI ACGGC 1 cut(s) 58
BisI GCNGC 4 cut(s) 92, 162, 230, 254
BlsI GCNGC 4 cut(s) 93, 163, 231, 255
BmeRI GACNNNNNGTC 1 cut(s) 121
BmiI GGNNCC 1 cut(s) 201
BmsI GCATC 1 cut(s) 273
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 216, 268
BsaXI ACNNNNNCTCC 2 cut(s) 15, 45
Bsc4I CCNNNNNNNGG 1 cut(s) 193
BseDI CCNNGG 2 cut(s) 216, 268
BseGI GGATG 1 cut(s) 264
BseLI CCNNNNNNNGG 1 cut(s) 193
BseRI GAGGAG 2 cut(s) 267, 276
BseXI GCAGC 3 cut(s) 103, 241, 265
BsgI GTGCAG 1 cut(s) 110
Bsh1236I CGCG 2 cut(s) 105, 119
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 1 cut(s) 260
BshNI GGYRCC 1 cut(s) 199
BsiEI CGRYCG 1 cut(s) 114
BsiHKAI GWGCWC 1 cut(s) 152
BslI CCNNNNNNNGG 1 cut(s) 193
BsnI GGCC 1 cut(s) 260
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 4 cut(s) 84, 111, 189, 220
Bsp19I CCATGG 1 cut(s) 216
BspACI CCGC 2 cut(s) 161, 291
BspANI GGCC 1 cut(s) 260
BspFNI CGCG 2 cut(s) 105, 119
BspLI GGNNCC 1 cut(s) 201
BspPI GGATC 1 cut(s) 228
BspQI GCTCTTC 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 199
BssECI CCNNGG 2 cut(s) 216, 268
BssMI GATC 4 cut(s) 84, 111, 189, 220
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 216
Bst4CI ACNGT 1 cut(s) 27
Bst6I CTCTTC 1 cut(s) 140
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 4 cut(s) 59, 141, 258, 262
BstDSI CCRYGG 1 cut(s) 216
BstF5I GGATG 1 cut(s) 264
BstFNI CGCG 2 cut(s) 105, 119
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 4 cut(s) 87, 114, 192, 223
BstMBI GATC 4 cut(s) 84, 111, 189, 220
BstMCI CGRYCG 1 cut(s) 114
BstMWI GCNNNNNNNGC 3 cut(s) 28, 100, 116
BstNSI RCATGY 1 cut(s) 61
BstUI CGCG 2 cut(s) 105, 119
BstV1I GCAGC 3 cut(s) 103, 241, 265
BsuRI GGCC 1 cut(s) 260
BtgI CCRYGG 1 cut(s) 216
BtgZI GCGATG 1 cut(s) 196
BtsCI GGATG 1 cut(s) 264
Cac8I GCNNGC 4 cut(s) 59, 141, 258, 262
CfoI GCGC 1 cut(s) 105
CseI GACGC 2 cut(s) 108, 125
CviAII CATG 2 cut(s) 58, 217
CviJI RGCY 2 cut(s) 139, 260
CviKI_1 RGCY 2 cut(s) 139, 260
DpnI GATC 4 cut(s) 86, 113, 191, 222
DpnII GATC 4 cut(s) 84, 111, 189, 220
DriI GACNNNNNGTC 1 cut(s) 121
Eam1104I CTCTTC 1 cut(s) 140
Eam1105I GACNNNNNGTC 1 cut(s) 121
EarI CTCTTC 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 216
EcoT14I CCWWGG 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 2 cut(s) 61, 220
FaiI YATR 2 cut(s) 59, 218
FatI CATG 2 cut(s) 57, 216
FauI CCCGC 1 cut(s) 298
Fnu4HI GCNGC 4 cut(s) 92, 162, 230, 254
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 4 cut(s) 92, 162, 230, 254
GlaI GCGC 1 cut(s) 104
GluI GCNGC 4 cut(s) 92, 162, 230, 254
HaeIII GGCC 1 cut(s) 260
HgaI GACGC 2 cut(s) 108, 125
HhaI GCGC 1 cut(s) 105
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 61, 220
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HphI GGTGA 1 cut(s) 139
Hpy188I TCNGA 3 cut(s) 10, 35, 82
Hpy188III TCNNGA 2 cut(s) 187, 224
Hpy99I CGWCG 4 cut(s) 119, 124, 127, 184
HpyAV CCTTC 1 cut(s) 151
HpyCH4III ACNGT 1 cut(s) 27
HpyCH4IV ACGT 1 cut(s) 182
HpyCH4V TGCA 2 cut(s) 91, 253
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 100, 116
HpySE526I ACGT 1 cut(s) 182
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 61, 220
HspAI GCGC 1 cut(s) 103
Kzo9I GATC 4 cut(s) 84, 111, 189, 220
LguI GCTCTTC 1 cut(s) 140
LmnI GCTCC 1 cut(s) 36
LpnPI CCDG 4 cut(s) 125, 200, 242, 274
Lsp1109I GCAGC 3 cut(s) 103, 241, 265
LweI GCATC 1 cut(s) 273
MaeII ACGT 1 cut(s) 182
MaeIII GTNAC 1 cut(s) 127
MalI GATC 4 cut(s) 86, 113, 191, 222
MboI GATC 4 cut(s) 84, 111, 189, 220
MboII GAAGA 3 cut(s) 59, 145, 157
MhlI GDGCHC 1 cut(s) 152
MluI ACGCGT 1 cut(s) 117
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 6 cut(s) 4, 60, 278, 288, 294, 297
MslI CAYNNNNRTG 1 cut(s) 215
MvnI CGCG 2 cut(s) 105, 119
MwoI GCNNNNNNNGC 3 cut(s) 28, 100, 116
NcoI CCATGG 1 cut(s) 216
NdeII GATC 4 cut(s) 84, 111, 189, 220
NlaIII CATG 2 cut(s) 61, 220
NlaIV GGNNCC 1 cut(s) 201
NmuCI GTSAC 1 cut(s) 127
NspI RCATGY 1 cut(s) 61
PaeI GCATGC 1 cut(s) 61
PciSI GCTCTTC 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 128
PkrI GCNGC 4 cut(s) 93, 163, 231, 255
Ple19I CGATCG 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 201
PvuI CGATCG 1 cut(s) 114
RseI CAYNNNNRTG 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 140
SatI GCNGC 4 cut(s) 92, 162, 230, 254
Sau3AI GATC 4 cut(s) 84, 111, 189, 220
SduI GDGCHC 1 cut(s) 152
SetI ASST 2 cut(s) 141, 185
SfaNI GCATC 1 cut(s) 273
SmiMI CAYNNNNRTG 1 cut(s) 215
SphI GCATGC 1 cut(s) 61
SsiI CCGC 2 cut(s) 161, 291
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 1 cut(s) 27
TaiI ACGT 1 cut(s) 185
TaqI TCGA 2 cut(s) 65, 114
TauI GCSGC 1 cut(s) 164
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 3 cut(s) 91, 229, 253
Tsp45I GTSAC 1 cut(s) 127
XceI RCATGY 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.