Rw0G003270

Mitochondrial import receptor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00113
OG0005799
Physical Location & Seq
Forward (+)
9361 .. 9935
575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G003270.1

Sequence Viewer

Length: 276 bp
ATGGCGTCTGAAGGCAGAAAGCGAGTGGCTGTGGGCAATGACAAAGAGGAACAGGGGCTGATCACGAGGCTGTCACAGTCGACGGTGGTGCAGAAGGCCAAGGGGTTGGCAAACGACGGGTCGTATGTGGCGAAGAAGCTGGTGAAGAGCACGGGGAAGGCGGCGTGGATCTTCGGCACGACGTTTCTGATCCTGGTCGTCCCTATCATGGTCCAGACGGACCGAGAGCAGCAGGTCTTCGAATATGAGTCGCAGCAGGGCATCCTCGGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tom22

Protein Analysis

91

Amino Acids

9.97

Weight (kDa)

9.57

Isoelectric Point (pI)

35.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tom22 PF04281 37 - 86 1.2e-07 Mitochondrial import receptor subunit Tom22
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 223
AccI GTMKAC 1 cut(s) 80
AciI CCGC 1 cut(s) 161
AclWI GGATC 2 cut(s) 176, 184
AcuI CTGAAG 1 cut(s) 30
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 208
AjnI CCWGG 1 cut(s) 192
AluBI AGCT 1 cut(s) 139
AluI AGCT 1 cut(s) 139
Alw21I GWGCWC 1 cut(s) 152
AlwI GGATC 2 cut(s) 176, 184
AlwNI CAGNNNCTG 1 cut(s) 58
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 2 cut(s) 229, 253
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
AspS9I GGNCC 2 cut(s) 211, 220
AsuHPI GGTGA 1 cut(s) 154
AsuII TTCGAA 1 cut(s) 240
AvaII GGWCC 2 cut(s) 211, 220
BauI CACGAG 1 cut(s) 64
BbsI GAAGAC 1 cut(s) 229
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 2 cut(s) 241, 265
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciT130I CCWGG 1 cut(s) 194
BclI TGATCA 1 cut(s) 60
BfuAI ACCTGC 1 cut(s) 223
BisI GCNGC 3 cut(s) 162, 230, 254
BlsI GCNGC 3 cut(s) 163, 231, 255
Bme1390I CCNGG 1 cut(s) 194
Bme18I GGWCC 2 cut(s) 211, 220
BmgT120I GGNCC 2 cut(s) 211, 220
BmrFI CCNGG 1 cut(s) 194
BmsI GCATC 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 229
Bpu14I TTCGAA 1 cut(s) 240
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 99, 265
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse3DI GCAATG 1 cut(s) 43
BseBI CCWGG 1 cut(s) 194
BseDI CCNNGG 2 cut(s) 99, 265
BseGI GGATG 1 cut(s) 261
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMI GCAATG 1 cut(s) 43
BseXI GCAGC 2 cut(s) 241, 265
BsgI GTGCAG 1 cut(s) 110
BshFI GGCC 1 cut(s) 98
BsiHKAI GWGCWC 1 cut(s) 152
BslFI GGGAC 1 cut(s) 185
BslI CCNNNNNNNGG 1 cut(s) 208
BsmFI GGGAC 1 cut(s) 185
BsnI GGCC 1 cut(s) 98
Bsp119I TTCGAA 1 cut(s) 240
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 3 cut(s) 60, 168, 189
BspACI CCGC 1 cut(s) 161
BspANI GGCC 1 cut(s) 98
BspMI ACCTGC 1 cut(s) 223
BspPI GGATC 2 cut(s) 176, 184
BspQI GCTCTTC 1 cut(s) 140
BspT104I TTCGAA 1 cut(s) 240
BsrDI GCAATG 1 cut(s) 43
BssECI CCNNGG 2 cut(s) 99, 265
BssMI GATC 3 cut(s) 60, 168, 189
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 64
BssT1I CCWWGG 1 cut(s) 99
Bst2BI CACGAG 1 cut(s) 64
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 2 cut(s) 78, 85
Bst6I CTCTTC 1 cut(s) 140
BstACI GRCGYC 1 cut(s) 5
BstBI TTCGAA 1 cut(s) 240
BstF5I GGATG 1 cut(s) 261
BstKTI GATC 3 cut(s) 63, 171, 192
BstMBI GATC 3 cut(s) 60, 168, 189
BstMWI GCNNNNNNNGC 1 cut(s) 267
BstNI CCWGG 1 cut(s) 194
BstSCI CCNGG 1 cut(s) 192
BstV1I GCAGC 2 cut(s) 241, 265
BstV2I GAAGAC 1 cut(s) 229
BstX2I RGATCY 1 cut(s) 168
BstXI CCANNNNNNTGG 1 cut(s) 106
BstYI RGATCY 1 cut(s) 168
BsuRI GGCC 1 cut(s) 98
BtsCI GGATG 1 cut(s) 261
BveI ACCTGC 1 cut(s) 223
CaiI CAGNNNCTG 1 cut(s) 58
Cfr13I GGNCC 2 cut(s) 211, 220
CpoI CGGWCCG 1 cut(s) 220
CspI CGGWCCG 1 cut(s) 220
CviAII CATG 1 cut(s) 208
CviJI RGCY 5 cut(s) 29, 58, 70, 98, 139
CviKI_1 RGCY 5 cut(s) 29, 58, 70, 98, 139
DpnI GATC 3 cut(s) 62, 170, 191
DpnII GATC 3 cut(s) 60, 168, 189
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 99
Eco47I GGWCC 2 cut(s) 211, 220
Eco57I CTGAAG 1 cut(s) 30
EcoRII CCWGG 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 1 cut(s) 211
FaiI YATR 3 cut(s) 126, 209, 246
FaqI GGGAC 1 cut(s) 185
FatI CATG 1 cut(s) 207
FbaI TGATCA 1 cut(s) 60
FblI GTMKAC 1 cut(s) 80
Fnu4HI GCNGC 3 cut(s) 162, 230, 254
FokI GGATG 1 cut(s) 248
Fsp4HI GCNGC 3 cut(s) 162, 230, 254
GluI GCNGC 3 cut(s) 162, 230, 254
HaeIII GGCC 1 cut(s) 98
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 211
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HinfI GANTC 1 cut(s) 248
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 1 cut(s) 81
Hpy188I TCNGA 2 cut(s) 10, 189
Hpy188III TCNNGA 2 cut(s) 64, 214
Hpy8I GTNNAC 1 cut(s) 81
Hpy99I CGWCG 3 cut(s) 85, 119, 184
HpyAV CCTTC 3 cut(s) 5, 88, 151
HpyCH4III ACNGT 2 cut(s) 78, 85
HpyCH4IV ACGT 1 cut(s) 182
HpyCH4V TGCA 1 cut(s) 91
HpyF10VI GCNNNNNNNGC 1 cut(s) 267
HpySE526I ACGT 1 cut(s) 182
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 211
Ksp22I TGATCA 1 cut(s) 60
Kzo9I GATC 3 cut(s) 60, 168, 189
LguI GCTCTTC 1 cut(s) 140
LpnPI CCDG 7 cut(s) 38, 125, 179, 206, 218, 227, 242
Lsp1109I GCAGC 2 cut(s) 241, 265
LweI GCATC 1 cut(s) 270
MaeII ACGT 1 cut(s) 182
MaeIII GTNAC 1 cut(s) 72
MalI GATC 3 cut(s) 62, 170, 191
MboI GATC 3 cut(s) 60, 168, 189
MboII GAAGA 4 cut(s) 145, 157, 163, 229
MflI RGATCY 1 cut(s) 168
MhlI GDGCHC 1 cut(s) 152
MlyI GAGTC 1 cut(s) 257
MnlI CCTC 3 cut(s) 40, 60, 275
MspR9I CCNGG 1 cut(s) 194
MvaI CCWGG 1 cut(s) 194
MwoI GCNNNNNNNGC 1 cut(s) 267
NdeII GATC 3 cut(s) 60, 168, 189
NlaIII CATG 1 cut(s) 211
NmeAIII GCCGAG 1 cut(s) 246
NmuCI GTSAC 1 cut(s) 72
NspV TTCGAA 1 cut(s) 240
PciSI GCTCTTC 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 128
PkrI GCNGC 3 cut(s) 163, 231, 255
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
Psp6I CCWGG 1 cut(s) 192
PspGI CCWGG 1 cut(s) 192
PspPI GGNCC 2 cut(s) 211, 220
PstNI CAGNNNCTG 1 cut(s) 58
PsuI RGATCY 1 cut(s) 168
Rsr2I CGGWCCG 1 cut(s) 220
RsrII CGGWCCG 1 cut(s) 220
SalI GTCGAC 1 cut(s) 79
SapI GCTCTTC 1 cut(s) 140
SatI GCNGC 3 cut(s) 162, 230, 254
Sau3AI GATC 3 cut(s) 60, 168, 189
Sau96I GGNCC 2 cut(s) 211, 220
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 1 cut(s) 194
SduI GDGCHC 1 cut(s) 152
SetI ASST 3 cut(s) 141, 185, 237
SfaNI GCATC 1 cut(s) 270
SfuI TTCGAA 1 cut(s) 240
SinI GGWCC 2 cut(s) 211, 220
SsiI CCGC 1 cut(s) 161
StyD4I CCNGG 1 cut(s) 192
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 2 cut(s) 78, 85
TaiI ACGT 1 cut(s) 185
TaqI TCGA 2 cut(s) 80, 240
TaqII GACCGA 1 cut(s) 237
TauI GCSGC 1 cut(s) 164
TseFI GTSAC 1 cut(s) 72
TseI GCWGC 2 cut(s) 229, 253
Tsp45I GTSAC 1 cut(s) 72
TspGWI ACGGA 1 cut(s) 233
VpaK11BI GGWCC 2 cut(s) 211, 220
XmiI GTMKAC 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.