Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002949

Physical Location & Seq

Genomic Coordinates Forward (+)

25343329 .. 25351854

Gene Structure 8526 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1242900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2463 bp

ATGGTAAAAATGGTGGCCGTCAAGTTTGGGTGCGCAGTTGCTGTACTGTTTTCCCTTTTAATTGTTGGCAAAGCTGATGTTTACATTGTGACGATTGAAGGAGAGCCTATTATAAGTTACCAAGGTGGTGTTGATGGATTTGAAGCCACTGCAGTGGAATCTGATGAAAAGATTGACACAACTAGTGAATCAGTCACATCGTATGCCCGACACTTGGAGAGCAAACACGATATGCTTCTTGGCATGTTGTTTGAGGAAGGGAGCTACCAAAAACTCTACAGCTATCAACATCTTATTAATGGATTTGCTGTCCACATTTCTCATGAACAGGCAGAAACATTGATGCGTGCTCCTGGTGTGAAATCGGTGGAGAGAGATTGGAAGGTGAGGAGACTTACAACCCACACCCCACAGTTTTTAGGGCTCCCAACTGGAGTATGGCCGACAGGAGGTGGCTCCGATAGGGCTGGAGAAGATATTGTGATAGGGTTTGTCGACTCAGGGATTTATCCGCACCATCCAAGCTTTGCTAGCCACAATACCGATCCTTATGGGCCTGTTCCAAAGTATAGAGGTAAATGTGAAGTTGATCCAGACACCAAGAGGAGCTTCTGTAATGGGAAGATTATTGGAGCTCACCATTTTGCCAAAGCTGCAATAGCAGCTGGAGTATTTAATCCTTCTATTGATTTTGCCTCTCCTATGGACGGTGATGGACATGGAAGTCACACTGCAGCTATTGCAGCTGGTAATAATGGAATTCCAGTGAGAATGCATGGTCATGAATTTGGGAAAGCAAGTGGGATGGCTCCCCGTGCCAGAATTGCTGTATATAAAGCCCTTTACAGGCTTTTTGGAGGGTTTGTTGCAGATGTAGTAGCCGCAATTGATCAGGCTGTGTATGATGGAGTGGATATACTGAGCCTTTCTGTGGGGCCAAACAGTCCTCCAGCTACCACGAAGACCACTTATTTGAATCCTTTTGATGCCACACTTCTTTCAGCTGTGAAAGCAGGTGTGTTTGTTGCACAAGCAGCTGGAAATGGAGGACCTTTTCCAAAAACTTTGGTTTCTTATAGTCCTTGGATAGCTTCTGTTGCTGCCGCCATTGATGATCGCAGATACAAAAACCACCTAACGCTTGGAAATGGAAAAATATTAGCTGGACTTGGGTTGTCACCATCGACACATCCAAATCAAACATACACGCTGGTTGCTGCAAATGATGTGCTTCTTGATTCTTCTGTAGTGAAGTATAGCCCCTCAGACTGCCAGAAACCAGAAGTTTTAAACAAGAACTTGGTTCAGGGGAATGTTCTTCTTTGTGGCTATTCATTTAATTTCGTTGTTGGTACTGCATCAATCAAGAAAGTGTCCGAAACAGCCAAAAGTCTTGGTGCAATCGGTTTTGTTCTTGCGGTGGAAAATGTTTCCCCAGGGACAAAATTTGATCCTGTTCCTGTAGGCGTTCCTGGGATTCTAATCACTGATGTTGAAAAGTCAATGGATCTTATAGATTATTACAACATCTCAACTGTTAGGGATTGGACTGGTCGTGTGAAGAGCTTCAAAGCTATAGGTAGCATTGGGAATGGTTTGATGCCTATACTCCACAAGTCTGCACCACAGGTGGCACTATTCTCTGCTAGAGGACCCAACATCAAAGATTTCAGCTTCCAAGAAGCAGATCTTCTCAAACCGGATATTCTAGCTCCTGGTTCTCTGATTTGGGCTGCTTGGTCTCCAAATGGAACAGATGAACCAAATTATGTTGGGGAGGGATTTGCCATGATTTCTGGAACAAGTATGGCTGCACCACATATAGCGGGAATAGCTGCTCTTGTAAAGCAGAAGCACCCTCATTGGAGCCCCGCTGCCATCAAATCAGCTTTGATGACAACATCAACAACTATCGACAGGGCTGGAAAACCTCTTCAAGCACAACAATATTCTGAAACACAAACCATTAAGTTTGTCGGTGCCACCCCTTTTGATTATGGGAGTGGCCATGTTGATCCAAAAGCTGCTCTGGATCCTGGACTAATCTTTGATGCAGGCTATCAGGATTACTTGGGTTTCTTGTGTACAACAGCTGGTATTAATTCTAATGAGATAAAGAACTACACAAACTCCCCCTGCAACTACACTATGGGCCACCCGTCAAATTTCAACTCTCCGTCAATCACAGTAGCTCATCTTGTGAAAAGTCAAACAGTTACACGCACGGTCACAAATGTTGCTGAAGAAGAAACCTATGTGATCACAACCAGAATGGCTCCAGCCATTGCAATTGAAGCCAGCCCTCGGGCAATGACTCTGAGACCTGGTGCATCACGAAAGTTCTCAGTGACTCTGACAGTTCGGTCTATCACTGGAGCCTACAGTTTCGGAGAGGTGATAATGAAAGGTAGCCGGGGGCACAAGGTGAGGATCCCAGTAGTAGCTATGGGTTACCAACGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8 fn3_5

Protein Analysis

821

Amino Acids

87.48

Weight (kDa)

7.99

Isoelectric Point (pI)

27.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	28 - 130	5.4e-15	Peptidase inhibitor I9
Peptidase_S8	PF00082	157 - 640	7.3e-42	Subtilase family
fn3_5	PF06280	705 - 814	1.8e-17	Fn3-like domain
fn3_6	PF17766	722 - 814	9.2e-18	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002949)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19170 AT2G19170 AT4G30020 AT4G30020 AT4G30020 AT4G30020
fragaria_vesca	FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370
malus_domestica	MD13G1242900.v1.1 MD16G1249700.v1.1
prunus_persica	Prupe.1G076600_v2.0.a1 Prupe.1G076600_v2.0.a1 Prupe.1G076600_v2.0.a1 Prupe.1G076600_v2.0.a1
pyrus_communis	pycom13g21460 pycom16g20920 pycom16g20930
rosa_chinensis	RchiOBHm_Chr4g0399741 RchiOBHm_Chr4g0399751
rosa_laevigata	RLG00000009242
rosa_multiflora	Rmu_sc0005299.1_g000011 Rmu_sc0028740.1_g000001
rosa_roxburghii	Rroxscaffold_5G00344690
rosa_rugosa	Rorug04G0018500 Rorug04G0018600 Rorug04G0018700
rosa_samantha	Rh4AG096300 Rh4BG093700 Rh4CG105200 Rh4DG090100
rosa_wichuraiana	Rw4G007960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	113
AarI	CACCTGC	1 cut(s)	1004
AasI	GACNNNNNNGTC	2 cut(s)	723, 2362
Acc16I	TGCGCA	1 cut(s)	34
Acc36I	ACCTGC	1 cut(s)	1004
AccB1I	GGYRCC	1 cut(s)	1979
AccI	GTMKAC	1 cut(s)	495
AciI	CCGC	6 cut(s)	512, 882, 1104, 1418, 1826, 1872
AclWI	GGATC	9 cut(s)	539, 584, 1445, 1515, 2009, 2027, 2040, 2425, 2438
AcoI	YGGCCR	3 cut(s)	15, 440, 2005
AcsI	RAATTY	4 cut(s)	759, 785, 1445, 2164
AcuI	CTGAAG	1 cut(s)	2262
AdeI	CACNNNGTG	1 cut(s)	2425
AfaI	GTAC	3 cut(s)	45, 1354, 2086
AfiI	CCNNNNNNNGG	6 cut(s)	214, 449, 707, 846, 931, 2304
AgsI	TTSAA	8 cut(s)	98, 143, 976, 1496, 1570, 1937, 2170, 2294
AhlI	ACTAGT	1 cut(s)	182
AjnI	CCWGG	6 cut(s)	352, 1435, 1471, 1714, 2035, 2323
AjuI	GAANNNNNNNTTGG	2 cut(s)	593, 625
AleI	CACNNNNGTG	1 cut(s)	152
Alw21I	GWGCWC	2 cut(s)	352, 637
Alw26I	GTCTC	3 cut(s)	385, 1746, 2314
AlwI	GGATC	9 cut(s)	539, 584, 1445, 1515, 2009, 2027, 2040, 2425, 2438
AlwNI	CAGNNNCTG	1 cut(s)	41
Ama87I	CYCGRG	1 cut(s)	2304
AoxI	GGCC	6 cut(s)	15, 440, 554, 935, 2005, 2152
ApoI	RAATTY	4 cut(s)	759, 785, 1445, 2164
ArsI	GACNNNNNNTTYG	2 cut(s)	955, 987
AseI	ATTAAT	2 cut(s)	297, 2100
Asp700I	GAANNNNTTC	1 cut(s)	1565
AspLEI	GCGC	1 cut(s)	35
AspS9I	GGNCC	5 cut(s)	554, 935, 1049, 1652, 2152
AsuC2I	CCSGG	1 cut(s)	2414
AsuHPI	GGTGA	6 cut(s)	397, 629, 722, 1170, 2407, 2437
AsuNHI	GCTAGC	1 cut(s)	530
AvaI	CYCGRG	1 cut(s)	2304
AvaII	GGWCC	2 cut(s)	1049, 1652
BaeGI	GKGCMC	1 cut(s)	2421
BalI	TGGCCA	1 cut(s)	2007
BamHI	GGATCC	2 cut(s)	2032, 2430
BanI	GGYRCC	1 cut(s)	1979
BanII	GRGCYC	3 cut(s)	426, 637, 1871
BbsI	GAAGAC	1 cut(s)	968
Bbv12I	GWGCWC	2 cut(s)	352, 637
BccI	CCATC	7 cut(s)	128, 525, 707, 799, 899, 1189, 1886
BceAI	ACGGC	1 cut(s)	2
BciT130I	CCWGG	6 cut(s)	354, 1437, 1473, 1716, 2037, 2325
BclI	TGATCA	2 cut(s)	889, 2259
BcnI	CCSGG	1 cut(s)	2414
BcoDI	GTCTC	3 cut(s)	385, 1746, 2314
BcuI	ACTAGT	1 cut(s)	182
BfaI	CTAG	4 cut(s)	183, 531, 1647, 1709
BfmI	CTRYAG	7 cut(s)	150, 277, 732, 1245, 1461, 1575, 2380
BfuAI	ACCTGC	1 cut(s)	1004
BglII	AGATCT	1 cut(s)	1687
Bme1390I	CCNGG	7 cut(s)	354, 1437, 1473, 1716, 2037, 2325, 2414
Bme18I	GGWCC	2 cut(s)	1049, 1652
BmeT110I	CYCGRG	1 cut(s)	2304
BmgT120I	GGNCC	5 cut(s)	554, 935, 1049, 1652, 2152
BmrFI	CCNGG	7 cut(s)	354, 1437, 1473, 1716, 2037, 2325, 2414
BmrI	ACTGGG	1 cut(s)	2429
BmsI	GCATC	6 cut(s)	333, 976, 1367, 1590, 2041, 2339
BmtI	GCTAGC	1 cut(s)	534
BmuI	ACTGGG	1 cut(s)	2429
BpiI	GAAGAC	1 cut(s)	968
BpmI	CTGGAG	6 cut(s)	453, 489, 687, 933, 2262, 2394
BpuMI	CCSGG	1 cut(s)	2414
BsaBI	GATNNNNATC	1 cut(s)	1481
BsaI	GGTCTC	2 cut(s)	1746, 2314
BsaJI	CCNNGG	7 cut(s)	121, 1082, 1435, 1436, 1472, 2303, 2413
BsaWI	WCCGGW	1 cut(s)	1699
BsaXI	ACNNNNNCTCC	6 cut(s)	598, 628, 849, 879, 2114, 2144
Bsc4I	CCNNNNNNNGG	6 cut(s)	214, 449, 707, 846, 931, 2304
Bse1I	ACTGG	5 cut(s)	436, 764, 1555, 2377, 2435
Bse3DI	GCAATG	2 cut(s)	2283, 2316
Bse8I	GATNNNNATC	1 cut(s)	1481
BseBI	CCWGG	6 cut(s)	354, 1437, 1473, 1716, 2037, 2325
BseDI	CCNNGG	7 cut(s)	121, 1082, 1435, 1436, 1472, 2303, 2413
BseGI	GGATG	3 cut(s)	517, 810, 1189
BseJI	GATNNNNATC	1 cut(s)	1481
BseLI	CCNNNNNNNGG	6 cut(s)	214, 449, 707, 846, 931, 2304
BseMI	GCAATG	2 cut(s)	2283, 2316
BseMII	CTCAG	5 cut(s)	513, 911, 1278, 2309, 2358
BseNI	ACTGG	5 cut(s)	436, 764, 1555, 2377, 2435
BseRI	GAGGAG	2 cut(s)	403, 619
BseSI	GKGCMC	1 cut(s)	2421
BsgI	GTGCAG	2 cut(s)	1605, 1797
BshFI	GGCC	6 cut(s)	17, 442, 556, 937, 2007, 2154
BshNI	GGYRCC	1 cut(s)	1979
BsiHKAI	GWGCWC	2 cut(s)	352, 637
BsiHKCI	CYCGRG	1 cut(s)	2304
BsiSI	CCGG	2 cut(s)	1700, 2413
BslFI	GGGAC	1 cut(s)	1453
BslI	CCNNNNNNNGG	6 cut(s)	214, 449, 707, 846, 931, 2304
BsmAI	GTCTC	3 cut(s)	385, 1746, 2314
BsmFI	GGGAC	1 cut(s)	1453
BsmI	GAATGC	1 cut(s)	777
BsnI	GGCC	6 cut(s)	17, 442, 556, 937, 2007, 2154
Bso31I	GGTCTC	2 cut(s)	1746, 2314
BsoBI	CYCGRG	1 cut(s)	2304
Bsp1286I	GDGCHC	5 cut(s)	352, 426, 637, 1871, 2421
Bsp1407I	TGTACA	1 cut(s)	2084
BspACI	CCGC	6 cut(s)	512, 882, 1104, 1418, 1826, 1872
BspANI	GGCC	6 cut(s)	17, 442, 556, 937, 2007, 2154
BspCNI	CTCAG	5 cut(s)	512, 912, 1277, 2310, 2357
BspHI	TCATGA	2 cut(s)	322, 781
BspMAI	CTGCAG	2 cut(s)	154, 736
BspMI	ACCTGC	1 cut(s)	1004
BspOI	GCTAGC	1 cut(s)	534
BspPI	GGATC	9 cut(s)	539, 584, 1445, 1515, 2009, 2027, 2040, 2425, 2438
BspQI	GCTCTTC	1 cut(s)	1556
BspT107I	GGYRCC	1 cut(s)	1979
BspTNI	GGTCTC	2 cut(s)	1746, 2314
BsrDI	GCAATG	2 cut(s)	2283, 2316
BsrGI	TGTACA	1 cut(s)	2084
BsrI	ACTGG	5 cut(s)	436, 764, 1555, 2377, 2435
BssECI	CCNNGG	7 cut(s)	121, 1082, 1435, 1436, 1472, 2303, 2413
BssT1I	CCWWGG	2 cut(s)	121, 1082
Bst2UI	CCWGG	6 cut(s)	354, 1437, 1473, 1716, 2037, 2325
Bst6I	CTCTTC	2 cut(s)	1556, 1938
BstAPI	GCANNNNNTGC	1 cut(s)	740
BstAUI	TGTACA	1 cut(s)	2084
BstC8I	GCNNGC	4 cut(s)	348, 532, 2056, 2299
BstDEI	CTNAG	5 cut(s)	499, 920, 1264, 2318, 2344
BstEII	GGTNACC	1 cut(s)	2450
BstF5I	GGATG	3 cut(s)	517, 810, 1189
BstHHI	GCGC	1 cut(s)	35
BstMAI	GTCTC	3 cut(s)	385, 1746, 2314
BstNI	CCWGG	6 cut(s)	354, 1437, 1473, 1716, 2037, 2325
BstNSI	RCATGY	1 cut(s)	247
BstPI	GGTNACC	1 cut(s)	2450
BstSCI	CCNGG	7 cut(s)	352, 1435, 1471, 1714, 2035, 2323, 2412
BstSFI	CTRYAG	7 cut(s)	150, 277, 732, 1245, 1461, 1575, 2380
BstSLI	GKGCMC	1 cut(s)	2421
BstV2I	GAAGAC	1 cut(s)	968
BstX2I	RGATCY	4 cut(s)	1507, 1687, 2032, 2430
BstXI	CCANNNNNNTGG	1 cut(s)	154
BstYI	RGATCY	4 cut(s)	1507, 1687, 2032, 2430
BsuRI	GGCC	6 cut(s)	17, 442, 556, 937, 2007, 2154
BtsCI	GGATG	3 cut(s)	517, 810, 1189
BtsI	GCAGTG	3 cut(s)	147, 159, 729
BtsIMutI	CAGTG	7 cut(s)	147, 159, 729, 771, 1485, 2352, 2370
BveI	ACCTGC	1 cut(s)	1004
Cac8I	GCNNGC	4 cut(s)	348, 532, 2056, 2299
CaiI	CAGNNNCTG	1 cut(s)	41
CciI	TCATGA	2 cut(s)	322, 781
CfoI	GCGC	1 cut(s)	35
Cfr13I	GGNCC	5 cut(s)	554, 935, 1049, 1652, 2152
CsiI	ACCWGGT	1 cut(s)	2323
Csp6I	GTAC	3 cut(s)	44, 1353, 2085
CspCI	CAANNNNNGTGG	2 cut(s)	1972, 2007
CviAII	CATG	7 cut(s)	244, 323, 719, 776, 782, 1789, 2009
CviQI	GTAC	3 cut(s)	44, 1353, 2085
DdeI	CTNAG	5 cut(s)	499, 920, 1264, 2318, 2344
DraI	TTTAAA	1 cut(s)	1290
DraIII	CACNNNGTG	1 cut(s)	2425
DrdI	GACNNNNNNGTC	2 cut(s)	723, 2362
DseDI	GACNNNNNNGTC	2 cut(s)	723, 2362
EaeI	YGGCCR	3 cut(s)	15, 440, 2005
Eam1104I	CTCTTC	2 cut(s)	1556, 1938
EarI	CTCTTC	2 cut(s)	1556, 1938
Ecl136II	GAGCTC	1 cut(s)	635
Eco130I	CCWWGG	2 cut(s)	121, 1082
Eco24I	GRGCYC	3 cut(s)	426, 637, 1871
Eco31I	GGTCTC	2 cut(s)	1746, 2314
Eco47I	GGWCC	2 cut(s)	1049, 1652
Eco53kI	GAGCTC	1 cut(s)	635
Eco57I	CTGAAG	1 cut(s)	2262
Eco88I	CYCGRG	1 cut(s)	2304
Eco91I	GGTNACC	1 cut(s)	2450
EcoICRI	GAGCTC	1 cut(s)	635
EcoO109I	RGGNCCY	2 cut(s)	1049, 1652
EcoO65I	GGTNACC	1 cut(s)	2450
EcoRI	GAATTC	1 cut(s)	759
EcoRII	CCWGG	6 cut(s)	352, 1435, 1471, 1714, 2035, 2323
EcoT14I	CCWWGG	2 cut(s)	121, 1082
EcoT22I	ATGCAT	1 cut(s)	777
EcoT38I	GRGCYC	3 cut(s)	426, 637, 1871
ErhI	CCWWGG	2 cut(s)	121, 1082
FaeI	CATG	7 cut(s)	247, 326, 722, 779, 785, 1792, 2012
FalI	AAGNNNNNCTT	4 cut(s)	593, 625, 1674, 1706
FaqI	GGGAC	1 cut(s)	1453
FatI	CATG	7 cut(s)	243, 322, 718, 775, 781, 1788, 2008
FauI	CCCGC	2 cut(s)	1819, 1879
FbaI	TGATCA	2 cut(s)	889, 2259
FblI	GTMKAC	1 cut(s)	495
FokI	GGATG	3 cut(s)	504, 817, 1176
FriOI	GRGCYC	3 cut(s)	426, 637, 1871
FspBI	CTAG	4 cut(s)	183, 531, 1647, 1709
FspI	TGCGCA	1 cut(s)	34
GlaI	GCGC	1 cut(s)	34
GsuI	CTGGAG	6 cut(s)	453, 489, 687, 933, 2262, 2394
HaeIII	GGCC	6 cut(s)	17, 442, 556, 937, 2007, 2154
HapII	CCGG	2 cut(s)	1700, 2413
HhaI	GCGC	1 cut(s)	35
Hin1II	CATG	7 cut(s)	247, 326, 722, 779, 785, 1792, 2012
Hin6I	GCGC	1 cut(s)	33
HinP1I	GCGC	1 cut(s)	33
HincII	GTYRAC	1 cut(s)	496
HindII	GTYRAC	1 cut(s)	496
HindIII	AAGCTT	1 cut(s)	523
HinfI	GANTC	8 cut(s)	158, 188, 497, 976, 1238, 1477, 2314, 2350
HpaII	CCGG	2 cut(s)	1700, 2413
HphI	GGTGA	6 cut(s)	397, 629, 722, 1170, 2407, 2437
Hpy166II	GTNNAC	4 cut(s)	82, 313, 496, 2085
Hpy188I	TCNGA	9 cut(s)	163, 460, 1267, 1378, 1725, 1954, 2319, 2355, 2390
Hpy188III	TCNNGA	9 cut(s)	323, 593, 782, 1235, 1366, 1797, 2030, 2063, 2334
Hpy8I	GTNNAC	4 cut(s)	82, 313, 496, 2085
HpyAV	CCTTC	4 cut(s)	92, 251, 376, 690
HpyF3I	CTNAG	5 cut(s)	499, 920, 1264, 2318, 2344
Hsp92II	CATG	7 cut(s)	247, 326, 722, 779, 785, 1792, 2012
HspAI	GCGC	1 cut(s)	33
Ksp22I	TGATCA	2 cut(s)	889, 2259
LguI	GCTCTTC	1 cut(s)	1556
LweI	GCATC	6 cut(s)	333, 976, 1367, 1590, 2041, 2339
MabI	ACCWGGT	1 cut(s)	2323
MaeI	CTAG	4 cut(s)	183, 531, 1647, 1709
MaeIII	GTNAC	9 cut(s)	88, 116, 193, 725, 1176, 2215, 2227, 2347, 2450
MfeI	CAATTG	2 cut(s)	885, 2289
MflI	RGATCY	4 cut(s)	1507, 1687, 2032, 2430
MhlI	GDGCHC	5 cut(s)	352, 426, 637, 1871, 2421
MlsI	TGGCCA	1 cut(s)	2007
MluNI	TGGCCA	1 cut(s)	2007
MlyI	GAGTC	3 cut(s)	491, 2308, 2344
Mox20I	TGGCCA	1 cut(s)	2007
Mph1103I	ATGCAT	1 cut(s)	777
MroXI	GAANNNNTTC	1 cut(s)	1565
MscI	TGGCCA	1 cut(s)	2007
MseI	TTAA	7 cut(s)	59, 297, 675, 1289, 1338, 1968, 2100
MslI	CAYNNNNRTG	2 cut(s)	152, 780
Msp20I	TGGCCA	1 cut(s)	2007
MspA1I	CMGCKG	6 cut(s)	665, 746, 1004, 1037, 1874, 2093
MspI	CCGG	2 cut(s)	1700, 2413
MspR9I	CCNGG	7 cut(s)	354, 1437, 1473, 1716, 2037, 2325, 2414
MunI	CAATTG	2 cut(s)	885, 2289
Mva1269I	GAATGC	1 cut(s)	777
MvaI	CCWGG	6 cut(s)	354, 1437, 1473, 1716, 2037, 2325
NciI	CCSGG	1 cut(s)	2414
NheI	GCTAGC	1 cut(s)	530
NlaIII	CATG	7 cut(s)	247, 326, 722, 779, 785, 1792, 2012
NmuCI	GTSAC	6 cut(s)	88, 193, 725, 1176, 2227, 2347
NsbI	TGCGCA	1 cut(s)	34
NsiI	ATGCAT	1 cut(s)	777
NspI	RCATGY	1 cut(s)	247
OliI	CACNNNNGTG	1 cut(s)	152
PagI	TCATGA	2 cut(s)	322, 781
PaqCI	CACCTGC	1 cut(s)	1004
PasI	CCCWGGG	1 cut(s)	1436
PciSI	GCTCTTC	1 cut(s)	1556
PctI	GAATGC	1 cut(s)	777
PdmI	GAANNNNTTC	1 cut(s)	1565
PfeI	GAWTC	5 cut(s)	158, 188, 976, 1238, 1477
PfoI	TCCNGGA	1 cut(s)	2035
PleI	GAGTC	3 cut(s)	491, 2308, 2344
PpsI	GAGTC	3 cut(s)	491, 2308, 2344
PpuMI	RGGWCCY	2 cut(s)	1049, 1652
PshBI	ATTAAT	2 cut(s)	297, 2100
PsiI	TTATAA	1 cut(s)	113
Psp124BI	GAGCTC	1 cut(s)	637
Psp5II	RGGWCCY	2 cut(s)	1049, 1652
Psp6I	CCWGG	6 cut(s)	352, 1435, 1471, 1714, 2035, 2323
PspEI	GGTNACC	1 cut(s)	2450
PspGI	CCWGG	6 cut(s)	352, 1435, 1471, 1714, 2035, 2323
PspPI	GGNCC	5 cut(s)	554, 935, 1049, 1652, 2152
PspPPI	RGGWCCY	2 cut(s)	1049, 1652
PstI	CTGCAG	2 cut(s)	154, 736
PstNI	CAGNNNCTG	1 cut(s)	41
PsuI	RGATCY	4 cut(s)	1507, 1687, 2032, 2430
PvuII	CAGCTG	5 cut(s)	665, 746, 1004, 1037, 2093
RsaI	GTAC	3 cut(s)	45, 1354, 2086
RsaNI	GTAC	3 cut(s)	44, 1353, 2085
RseI	CAYNNNNRTG	2 cut(s)	152, 780
SacI	GAGCTC	1 cut(s)	637
SalI	GTCGAC	1 cut(s)	494
SapI	GCTCTTC	1 cut(s)	1556
SaqAI	TTAA	7 cut(s)	59, 297, 675, 1289, 1338, 1968, 2100
Sau96I	GGNCC	5 cut(s)	554, 935, 1049, 1652, 2152
SchI	GAGTC	3 cut(s)	491, 2308, 2344
ScrFI	CCNGG	7 cut(s)	354, 1437, 1473, 1716, 2037, 2325, 2414
SduI	GDGCHC	5 cut(s)	352, 426, 637, 1871, 2421
SexAI	ACCWGGT	1 cut(s)	2323
SfaNI	GCATC	6 cut(s)	333, 976, 1367, 1590, 2041, 2339
SfcI	CTRYAG	7 cut(s)	150, 277, 732, 1245, 1461, 1575, 2380
SinI	GGWCC	2 cut(s)	1049, 1652
SmiMI	CAYNNNNRTG	2 cut(s)	152, 780
SpeI	ACTAGT	1 cut(s)	182
SsiI	CCGC	6 cut(s)	512, 882, 1104, 1418, 1826, 1872
SspI	AATATT	2 cut(s)	1158, 1949
SspMI	CTAG	4 cut(s)	183, 531, 1647, 1709
SstI	GAGCTC	1 cut(s)	637
StyD4I	CCNGG	7 cut(s)	352, 1435, 1471, 1714, 2035, 2323, 2412
StyI	CCWWGG	2 cut(s)	121, 1082
TaqI	TCGA	3 cut(s)	495, 1184, 1914
TaqII	GACCGA	1 cut(s)	2352
TatI	WGTACW	2 cut(s)	43, 2084
TauI	GCSGC	2 cut(s)	884, 1106
TfiI	GAWTC	5 cut(s)	158, 188, 976, 1238, 1477
Tru1I	TTAA	7 cut(s)	59, 297, 675, 1289, 1338, 1968, 2100
Tru9I	TTAA	7 cut(s)	59, 297, 675, 1289, 1338, 1968, 2100
TscAI	CASTG	7 cut(s)	154, 159, 736, 771, 1492, 2352, 2377
TseFI	GTSAC	6 cut(s)	88, 193, 725, 1176, 2227, 2347
Tsp45I	GTSAC	6 cut(s)	88, 193, 725, 1176, 2227, 2347
TspDTI	ATGAA	6 cut(s)	180, 339, 798, 1323, 1773, 2417
TspGWI	ACGGA	1 cut(s)	2166
TspRI	CASTG	7 cut(s)	154, 159, 736, 771, 1492, 2352, 2377
VpaK11BI	GGWCC	2 cut(s)	1049, 1652
VspI	ATTAAT	2 cut(s)	297, 2100
XapI	RAATTY	4 cut(s)	759, 785, 1445, 2164
XceI	RCATGY	1 cut(s)	247
XcmI	CCANNNNNNNNNTGG	2 cut(s)	435, 1139
XmiI	GTMKAC	1 cut(s)	495
XmnI	GAANNNNTTC	1 cut(s)	1565
XspI	CTAG	4 cut(s)	183, 531, 1647, 1709
Zsp2I	ATGCAT	1 cut(s)	777

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.