Rorug04G0018600

Belongs to the peptidase S8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002949
Physical Location & Seq
Reverse (-)
2766299 .. 2767672
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0018600.1

Sequence Viewer

Length: 1293 bp
ATGGCGCAAATTCACTGCGATTCCTCATCAGCAGCAGAAACGGTGGCCAATATCGAAGAGCTTCTGTCTCAGATACTTGTATTGGTTCCAGCCTTATCTCTGATCCGTTTCAAGTGCGTCTCCAGGCACTGGCTCACTCTCATCTCCGACCCCGGATTTTTTCGCCGCCACTTACATCAAAAGTCGAAGATCTCCGGCTTCTTTTCTAGCCAAACCGAAGACGAGTCCTTCAAGTCCATTGCTCTTAGTGACCGCGAAATCCCATCTGGGAATCCCTTCAAAGCTATAAACGATTCCTTCGGTGATGGCTCCAGGTTGAAGATTATTCAATCCTGCAACGGCCTGTTCCTTTGCCTTCGCTACTCTAGGGATATTTCTCTTTTGCGTCAAGCATATGGTGTCAAAGATCATCCCGCATATGTTGTGAATCCCACAACCAACAAATTCTTGGCTCTTTATTCTCCAACCGTTATGAAAAATAAAAAATATGATAGGTTTTTGGTACGTTATGCTCTGGCTTTTGACCCTTCCATATCACCTTTTTACAAGGTGGTCTGCGTCACTAACTACTCGTTTTGGGGGCATTCCCACCACCTAGACATATATTCCTCTGAGACTGAAGAGTGGAAGGAGCTTCTCATGGTTCCTTTATTCCCCATCTATCCCATGCACTTTGACCACAGGAGCACGGAAGGGGCAGTATACTGTAATGGCAAGATTCATTGGATAAGACGCATAACCGACAGATATGGCTATGACTCATGGTGCCACTTCCGCGATGGTAGATTCATAAGAGATGAAGGTGACGTGTTACACTACTTTGACATAGATGAACAAAGTTTGCAACTCGTCGCTGCTGCTACTCCTGTTCCTGTGGCTGTAAGAGCTGATAGATATTTTGGCGAGTCTGGCGGCCATTTGTATTTGATTGAGTTTTACAAGCATTACGACCCCCAATTTGACATCATGGAGATGGAGAGGGACTACTCTTGCTGGTTTATCAAGCATCACGTTGATCTTAATCTGGTATTCGCAGCTCTTCCTAACACAATTTCTTTTGTTGTCTTGTGCCTCTCTCCAGAGGAGGAAAAGGATGCGGAGGAGGAGGATTCTTCATGTAACCTTTGGTTGCATATGCCTGGCAAAGTCATGTCTTATAATCTAAGGAATAAGACCTTCAAAACATCAGCGGCGCTGGCCGATAAGGAACTTTTTCTAGCTTTGGATAATGATCGATGCTCTCACATGGAGGCTGCTAACTATCCTTACATGGAGACTTTAGCTTGTGTGTGA

Protein Analysis

430

Amino Acids

49.66

Weight (kDa)

5.94

Isoelectric Point (pI)

58.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 104 - 251 1.6e-06 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1158
AccB1I GGYRCC 1 cut(s) 765
AccB7I CCANNNNNTGG 1 cut(s) 129
AccI GTMKAC 1 cut(s) 702
AccII CGCG 2 cut(s) 255, 777
AciI CCGC 7 cut(s) 166, 253, 414, 775, 912, 1097, 1190
AclWI GGATC 1 cut(s) 97
AcoI YGGCCR 3 cut(s) 45, 913, 1197
AcsI RAATTY 2 cut(s) 9, 443
AcuI CTGAAG 1 cut(s) 639
AfaI GTAC 1 cut(s) 504
AfiI CCNNNNNNNGG 1 cut(s) 129
AflIII ACRYGT 1 cut(s) 807
AgsI TTSAA 6 cut(s) 112, 232, 280, 319, 329, 1180
AjiI CACGTC 1 cut(s) 808
AjnI CCWGG 3 cut(s) 122, 311, 1138
AluBI AGCT 7 cut(s) 61, 284, 634, 887, 1037, 1220, 1283
AluI AGCT 7 cut(s) 61, 284, 634, 887, 1037, 1220, 1283
Alw21I GWGCWC 1 cut(s) 689
Alw26I GTCTC 4 cut(s) 72, 124, 608, 1268
AlwI GGATC 1 cut(s) 97
AlwNI CAGNNNCTG 1 cut(s) 129
AoxI GGCC 4 cut(s) 45, 340, 913, 1197
ApeKI GCWGC 5 cut(s) 32, 854, 857, 1034, 1253
ApoI RAATTY 2 cut(s) 9, 443
ArsI GACNNNNNNTTYG 2 cut(s) 941, 973
Asp700I GAANNNNTTC 3 cut(s) 60, 275, 1212
AspLEI GCGC 2 cut(s) 7, 1195
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 3 cut(s) 314, 528, 815
BalI TGGCCA 1 cut(s) 47
BanI GGYRCC 1 cut(s) 765
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 689
BbvI GCAGC 5 cut(s) 44, 841, 844, 1046, 1240
BccI CCATC 5 cut(s) 271, 299, 665, 773, 967
BceAI ACGGC 1 cut(s) 355
BciT130I CCWGG 3 cut(s) 124, 313, 1140
BcnI CCSGG 1 cut(s) 153
BcoDI GTCTC 4 cut(s) 72, 124, 608, 1268
BfaI CTAG 4 cut(s) 207, 366, 596, 1217
BfoI RGCGCY 1 cut(s) 1196
BglII AGATCT 1 cut(s) 189
BisI GCNGC 8 cut(s) 33, 166, 855, 858, 913, 1035, 1191, 1254
BlsI GCNGC 8 cut(s) 34, 167, 856, 859, 914, 1036, 1192, 1255
Bme1390I CCNGG 4 cut(s) 124, 153, 313, 1140
BmgBI CACGTC 1 cut(s) 808
BmiI GGNNCC 4 cut(s) 87, 310, 645, 767
BmrFI CCNGG 4 cut(s) 124, 153, 313, 1140
BmsI GCATC 3 cut(s) 1015, 1084, 1226
BpiI GAAGAC 1 cut(s) 225
BpmI CTGGAG 3 cut(s) 106, 295, 1062
BpuMI CCSGG 1 cut(s) 153
Bsa29I ATCGAT 1 cut(s) 1234
BsaBI GATNNNNATC 2 cut(s) 1020, 1230
BsaJI CCNNGG 1 cut(s) 151
Bsc4I CCNNNNNNNGG 1 cut(s) 129
Bse1I ACTGG 1 cut(s) 134
Bse3DI GCAATG 1 cut(s) 237
Bse8I GATNNNNATC 2 cut(s) 1020, 1230
BseBI CCWGG 3 cut(s) 124, 313, 1140
BseCI ATCGAT 1 cut(s) 1234
BseDI CCNNGG 1 cut(s) 151
BseGI GGATG 2 cut(s) 409, 1099
BseJI GATNNNNATC 2 cut(s) 1020, 1230
BseLI CCNNNNNNNGG 1 cut(s) 129
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 2 cut(s) 83, 603
BseNI ACTGG 1 cut(s) 134
BseRI GAGGAG 3 cut(s) 1097, 1115, 1118
BseXI GCAGC 5 cut(s) 44, 841, 844, 1046, 1240
Bsh1236I CGCG 2 cut(s) 255, 777
BshFI GGCC 4 cut(s) 47, 342, 915, 1199
BshNI GGYRCC 1 cut(s) 765
BshVI ATCGAT 1 cut(s) 1234
BsiHKAI GWGCWC 1 cut(s) 689
BsiSI CCGG 2 cut(s) 153, 195
BslFI GGGAC 1 cut(s) 995
BslI CCNNNNNNNGG 1 cut(s) 129
BsmAI GTCTC 4 cut(s) 72, 124, 608, 1268
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 1 cut(s) 995
BsmI GAATGC 1 cut(s) 583
BsnI GGCC 4 cut(s) 47, 342, 915, 1199
Bsp1286I GDGCHC 1 cut(s) 689
Bsp143I GATC 5 cut(s) 102, 189, 406, 1015, 1231
BspACI CCGC 7 cut(s) 166, 253, 414, 775, 912, 1097, 1190
BspANI GGCC 4 cut(s) 47, 342, 915, 1199
BspCNI CTCAG 2 cut(s) 82, 604
BspDI ATCGAT 1 cut(s) 1234
BspFNI CGCG 2 cut(s) 255, 777
BspLI GGNNCC 4 cut(s) 87, 310, 645, 767
BspPI GGATC 1 cut(s) 97
BspQI GCTCTTC 2 cut(s) 51, 1044
BspT107I GGYRCC 1 cut(s) 765
BsrDI GCAATG 1 cut(s) 237
BsrI ACTGG 1 cut(s) 134
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 5 cut(s) 102, 189, 406, 1015, 1231
BssNAI GTATAC 1 cut(s) 703
Bst1107I GTATAC 1 cut(s) 703
Bst2UI CCWGG 3 cut(s) 124, 313, 1140
Bst4CI ACNGT 3 cut(s) 43, 469, 707
Bst6I CTCTTC 3 cut(s) 51, 615, 1044
BstC8I GCNNGC 1 cut(s) 1197
BstDEI CTNAG 4 cut(s) 69, 245, 612, 1163
BstF5I GGATG 2 cut(s) 409, 1099
BstFNI CGCG 2 cut(s) 255, 777
BstH2I RGCGCY 1 cut(s) 1196
BstHHI GCGC 2 cut(s) 7, 1195
BstKTI GATC 5 cut(s) 105, 192, 409, 1018, 1234
BstMAI GTCTC 4 cut(s) 72, 124, 608, 1268
BstMBI GATC 5 cut(s) 102, 189, 406, 1015, 1231
BstMWI GCNNNNNNNGC 4 cut(s) 774, 884, 909, 1196
BstNI CCWGG 3 cut(s) 124, 313, 1140
BstSCI CCNGG 4 cut(s) 122, 151, 311, 1138
BstUI CGCG 2 cut(s) 255, 777
BstV1I GCAGC 5 cut(s) 44, 841, 844, 1046, 1240
BstV2I GAAGAC 1 cut(s) 225
BstX2I RGATCY 1 cut(s) 189
BstYI RGATCY 1 cut(s) 189
BstZ17I GTATAC 1 cut(s) 703
Bsu15I ATCGAT 1 cut(s) 1234
BsuRI GGCC 4 cut(s) 47, 342, 915, 1199
BsuTUI ATCGAT 1 cut(s) 1234
BtgZI GCGATG 1 cut(s) 792
BtrI CACGTC 1 cut(s) 808
BtsCI GGATG 2 cut(s) 409, 1099
BtsI GCAGTG 1 cut(s) 13
BtsIMutI CAGTG 2 cut(s) 13, 127
Cac8I GCNNGC 1 cut(s) 1197
CaiI CAGNNNCTG 1 cut(s) 129
CfoI GCGC 2 cut(s) 7, 1195
ClaI ATCGAT 1 cut(s) 1234
CseI GACGC 4 cut(s) 106, 374, 547, 741
Csp6I GTAC 1 cut(s) 503
CviAII CATG 8 cut(s) 640, 667, 762, 967, 1116, 1150, 1246, 1270
CviQI GTAC 1 cut(s) 503
DdeI CTNAG 4 cut(s) 69, 245, 612, 1163
DpnI GATC 5 cut(s) 104, 191, 408, 1017, 1233
DpnII GATC 5 cut(s) 102, 189, 406, 1015, 1231
EaeI YGGCCR 3 cut(s) 45, 913, 1197
Eam1104I CTCTTC 3 cut(s) 51, 615, 1044
EarI CTCTTC 3 cut(s) 51, 615, 1044
Eco57I CTGAAG 1 cut(s) 639
EcoRII CCWGG 3 cut(s) 122, 311, 1138
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 8 cut(s) 643, 670, 765, 970, 1119, 1153, 1249, 1273
FaqI GGGAC 1 cut(s) 995
FatI CATG 8 cut(s) 639, 666, 761, 966, 1115, 1149, 1245, 1269
FauI CCCGC 1 cut(s) 421
FauNDI CATATG 3 cut(s) 394, 418, 1134
FblI GTMKAC 1 cut(s) 702
Fnu4HI GCNGC 8 cut(s) 33, 166, 855, 858, 913, 1035, 1191, 1254
FokI GGATG 2 cut(s) 396, 1106
Fsp4HI GCNGC 8 cut(s) 33, 166, 855, 858, 913, 1035, 1191, 1254
FspBI CTAG 4 cut(s) 207, 366, 596, 1217
GlaI GCGC 2 cut(s) 6, 1194
GluI GCNGC 8 cut(s) 33, 166, 855, 858, 913, 1035, 1191, 1254
GsuI CTGGAG 3 cut(s) 106, 295, 1062
HaeII RGCGCY 1 cut(s) 1196
HaeIII GGCC 4 cut(s) 47, 342, 915, 1199
HapII CCGG 2 cut(s) 153, 195
HgaI GACGC 4 cut(s) 106, 374, 547, 741
HhaI GCGC 2 cut(s) 7, 1195
Hin1II CATG 8 cut(s) 643, 670, 765, 970, 1119, 1153, 1249, 1273
Hin6I GCGC 2 cut(s) 5, 1193
HinP1I GCGC 2 cut(s) 5, 1193
HpaII CCGG 2 cut(s) 153, 195
HphI GGTGA 3 cut(s) 314, 528, 815
Hpy166II GTNNAC 1 cut(s) 703
Hpy188I TCNGA 4 cut(s) 72, 102, 148, 613
Hpy188III TCNNGA 1 cut(s) 1079
Hpy8I GTNNAC 1 cut(s) 703
Hpy99I CGWCG 1 cut(s) 854
HpyAV CCTTC 9 cut(s) 238, 286, 307, 365, 537, 622, 686, 794, 1186
HpyCH4III ACNGT 3 cut(s) 43, 469, 707
HpyCH4IV ACGT 3 cut(s) 505, 807, 1011
HpyCH4V TGCA 4 cut(s) 336, 670, 844, 1132
HpyF10VI GCNNNNNNNGC 4 cut(s) 774, 884, 909, 1196
HpyF3I CTNAG 4 cut(s) 69, 245, 612, 1163
HpySE526I ACGT 3 cut(s) 505, 807, 1011
Hsp92II CATG 8 cut(s) 643, 670, 765, 970, 1119, 1153, 1249, 1273
HspAI GCGC 2 cut(s) 5, 1193
Kzo9I GATC 5 cut(s) 102, 189, 406, 1015, 1231
LguI GCTCTTC 2 cut(s) 51, 1044
LmnI GCTCC 3 cut(s) 314, 631, 684
Lsp1109I GCAGC 5 cut(s) 44, 841, 844, 1046, 1240
LweI GCATC 3 cut(s) 1015, 1084, 1226
MaeI CTAG 4 cut(s) 207, 366, 596, 1217
MaeII ACGT 3 cut(s) 505, 807, 1011
MaeIII GTNAC 5 cut(s) 248, 559, 803, 810, 1118
MalI GATC 5 cut(s) 104, 191, 408, 1017, 1233
MboI GATC 5 cut(s) 102, 189, 406, 1015, 1231
MboII GAAGA 7 cut(s) 68, 199, 230, 331, 632, 1031, 1104
MflI RGATCY 1 cut(s) 189
MhlI GDGCHC 1 cut(s) 689
MlsI TGGCCA 1 cut(s) 47
MluCI AATT 4 cut(s) 9, 443, 956, 1050
MluNI TGGCCA 1 cut(s) 47
MlyI GAGTC 3 cut(s) 233, 752, 914
MmeI TCCRAC 2 cut(s) 171, 488
Mox20I TGGCCA 1 cut(s) 47
MroXI GAANNNNTTC 3 cut(s) 60, 275, 1212
MscI TGGCCA 1 cut(s) 47
MseI TTAA 1 cut(s) 1020
MslI CAYNNNNRTG 1 cut(s) 971
Msp20I TGGCCA 1 cut(s) 47
MspA1I CMGCKG 1 cut(s) 1190
MspI CCGG 2 cut(s) 153, 195
MspR9I CCNGG 4 cut(s) 124, 153, 313, 1140
Mva1269I GAATGC 1 cut(s) 583
MvaI CCWGG 3 cut(s) 124, 313, 1140
MvnI CGCG 2 cut(s) 255, 777
MwoI GCNNNNNNNGC 4 cut(s) 774, 884, 909, 1196
NciI CCSGG 1 cut(s) 153
NdeI CATATG 3 cut(s) 394, 418, 1134
NdeII GATC 5 cut(s) 102, 189, 406, 1015, 1231
NlaIII CATG 8 cut(s) 643, 670, 765, 970, 1119, 1153, 1249, 1273
NlaIV GGNNCC 4 cut(s) 87, 310, 645, 767
NmuCI GTSAC 3 cut(s) 248, 559, 803
PciSI GCTCTTC 2 cut(s) 51, 1044
PctI GAATGC 1 cut(s) 583
PdmI GAANNNNTTC 3 cut(s) 60, 275, 1212
PfeI GAWTC 7 cut(s) 20, 271, 293, 427, 718, 786, 1109
PflMI CCANNNNNTGG 1 cut(s) 129
PkrI GCNGC 8 cut(s) 34, 167, 856, 859, 914, 1036, 1192, 1255
PleI GAGTC 3 cut(s) 232, 752, 913
PpsI GAGTC 3 cut(s) 232, 752, 913
PsiI TTATAA 1 cut(s) 1158
Psp6I CCWGG 3 cut(s) 122, 311, 1138
PspGI CCWGG 3 cut(s) 122, 311, 1138
PspN4I GGNNCC 4 cut(s) 87, 310, 645, 767
PstNI CAGNNNCTG 1 cut(s) 129
PsuI RGATCY 1 cut(s) 189
RsaI GTAC 1 cut(s) 504
RsaNI GTAC 1 cut(s) 503
RseI CAYNNNNRTG 1 cut(s) 971
SapI GCTCTTC 2 cut(s) 51, 1044
SaqAI TTAA 1 cut(s) 1020
SatI GCNGC 8 cut(s) 33, 166, 855, 858, 913, 1035, 1191, 1254
Sau3AI GATC 5 cut(s) 102, 189, 406, 1015, 1231
SchI GAGTC 3 cut(s) 233, 752, 914
ScrFI CCNGG 4 cut(s) 124, 153, 313, 1140
SduI GDGCHC 1 cut(s) 689
SfaNI GCATC 3 cut(s) 1015, 1084, 1226
SmiMI CAYNNNNRTG 1 cut(s) 971
Sse9I AATT 4 cut(s) 9, 443, 956, 1050
SsiI CCGC 7 cut(s) 166, 253, 414, 775, 912, 1097, 1190
SspMI CTAG 4 cut(s) 207, 366, 596, 1217
StyD4I CCNGG 4 cut(s) 122, 151, 311, 1138
TaaI ACNGT 3 cut(s) 43, 469, 707
TaiI ACGT 3 cut(s) 508, 810, 1014
TaqI TCGA 3 cut(s) 54, 185, 1234
TasI AATT 4 cut(s) 9, 443, 956, 1050
TauI GCSGC 3 cut(s) 168, 915, 1193
TfiI GAWTC 7 cut(s) 20, 271, 293, 427, 718, 786, 1109
Tru1I TTAA 1 cut(s) 1020
Tru9I TTAA 1 cut(s) 1020
TscAI CASTG 2 cut(s) 20, 134
TseFI GTSAC 3 cut(s) 248, 559, 803
TseI GCWGC 5 cut(s) 32, 854, 857, 1034, 1253
Tsp45I GTSAC 3 cut(s) 248, 559, 803
TspDTI ATGAA 6 cut(s) 488, 710, 778, 813, 846, 1104
TspGWI ACGGA 2 cut(s) 95, 704
TspRI CASTG 2 cut(s) 20, 134
Van91I CCANNNNNTGG 1 cut(s) 129
XapI RAATTY 2 cut(s) 9, 443
XcmI CCANNNNNNNNNTGG 2 cut(s) 445, 776
XmiI GTMKAC 1 cut(s) 702
XmnI GAANNNNTTC 3 cut(s) 60, 275, 1212
XspI CTAG 4 cut(s) 207, 366, 596, 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.