Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0002949

Physical Location & Seq

Genomic Coordinates Forward (+)

13635058 .. 13654261

Gene Structure 19204 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG090100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2685 bp

ATGGTGTTTTCCCTGCAGCCATGGCCGCCGCCGTCATCTATTCCCCTCCCAGTTACACCTATTCCAAGGACAGTCCTAAGTCCACCAAATAAGGCATTGCTCGGGGCATACGGCATGGAAGTGGAAGTTAGAAGCTTGGCTCTTTTTTTGGTTCAAATATGCCTCTCAGCCATTTCTGGATCTCTCTGCAAATCTTGTGGAGTGCTGAAGTTGCATACTTCAATGGTAAAACTGAGGGCTATGGAGTTTTGGTATGCCGTTTTCGTATTGTTTTCCCTCTTCATTATTGGGAAAGCCGATGTTTATATTGTGACTGTCGAAGGAGAGCCTATTATAAGTTATAGAGGTGATGTTGATGGTTTTGAAGCCACTGCTGTGGAGTCTGATGAAAAGATCGACACCACTAGTGAATCAGTCACGTCCTATGCTCGACACCTTGAGAATAAACATGATATGCTTCTTGGAATGCTGTTTGAGCAAGGGACCTACAAGAAACTCTACAGCTATCAGCATCTTATAAATGGCTTTGCAGTTCATATTTCTCCCGAGCAGGCAGAAAGACTAATGCGTGCTCCGGGTGTGAAATCCGTGGAGAGAGATTGGAAGGTCCGGAGGCTTACAACTCACACACCACAGTTTTTAGGGCTTCCAACCGGAGTATGGCCAACAGGAGGTGGCTTTGATAGGGCTGGAGAAAATATTGTGATAGGGTTTGTGGACTCGGGGATTTACCCTCATCATCCAAGCTTTGCAAGCCACAGTACTGATCCTTACGGGCCTGTTCCCAAGTACAGAGGTACATGTGAAGTTGACCCAGACACCAAGAAGAGCTTCTGTAACGGAAAGATTATTGGAGCCCACCATTTCGCCAAAGCTGCAATAGCAGCTGGGGTGTTTAATCCAGTTATTGATTTCGCCTCTCCTATGGATGGTGATGGACATGGAAGTCACACTGCAGCTATTGCAGCTGGAAACAATGGAATTCCTGTCAGAATGCATGGCCATGAATTTGGCAAAGCAAGTGGGATGGCTCCCCGTGCCAGAATTGCTGTATATAAAGCTCTTTACAGGCTCTTTGGAGGGTTTGTAGCAGATGTAGTAGCCGCAATTGATCAGGCTGTTTATGATGGAGTGGATATACTGAGCCTTTCTGTGGGGCCAAACAGTCCTCCAGCTACCACCAAGACCACTTATTTGAACCCGTTTGATGCCACACTTCTTTCAGCTGTGAAAGCGGGTGTATTTGTTGCACAGGCAGCTGGAAATGGAGGACCTTTTCCAAAAACTTTGGTTTCTTATAGCCCTTGGATAGCTTCTGTTGCTGCTGCCATAGATGACCGGAGATACAAAAACCATTTCACGCTTGGGAATGGAAAAATATTACCTGGACTTGGGTTGTCACCATCTACACATCCAAATCGAACGTACACACTGATTGCTGCAAATGATGTGCTTTTGGATTCTTCAGTAGTGAAGTATAGCCCCTCAGACTGCCAGAGACCAGAAGTTTTAAACAAGAACTTGGTTGCGGGGAATATTCTTCTTTGTGGCTATTCGTTCAATTTTGTTGCTGGCTCTGCATCAATCAAGAAAGTCTCGGAAACAGCTAAAAGTCTTGGTGCAATTGGCTTTGTTCTTGCTGTGGAAAATGTTTCCCCCGGAACCAAATTTGATCCTGTTCCTTCTGGAATTCCTGGGATTGTAATCACTGATGTTAAGAAGTCAATGGATCTTATAGATTACTACAACATCTCCACATCAAGAGATTGGACTGGTCGAGTTAAGAGTTTCAAAGCTATAGGTAGCATTGGGGATGGTTTGATGCCTATACTCCACAAATCAGCACCCCAGGTGGCAATATTCTCTGCTCGAGGACCCAATATTAAAGATTTCAGCTTCCAAGATGCGGATCTTCTCAAACCAGATATTCTAGCTCCTGGTTCTCTGATTTGGGCTGCCTGGTCCCCTAATGGAACTGATGAGCCTGATTATGTTGGGGAGGGATTTGCCATGATTTCTGGAACAAGTATGGCTGCACCGCATATAGCAGGAATAGCTGCTCTTATAAAGCAGAAGCGCCCTCATTGGAGCCCTGCTGCCATCAAGTCATCCTTGATGACAACATCAACAACTATAGACCGGGCTGGAAGGCCTCTCCAGGCACAACAGACTTCCGAAACACAAGGCATTAAGTTTGTGAGTGCTACTCCTTTTGATTATGGTAGTGGCCATGTTGATCCAAGGGCTGCTCTAGATCCTGGACTAATCTTTGACGTGGGTTTTGAGGATTACGTGGGCTTCTTGTGTACAACACCTGGTATTGATGCCCATGAGATAAAGAAATACACAAACTCCCCCTGCAATTACACCATGGGCCACCCATCAAATTTCAACTCTCCGTCAATCACAGTAGCTCATCTTGTAGGAAGTCGTACAGTTACTCGCACAGTGACAAATGTTGCTCAAGAAGAAACCTATGTGATCACAGCCAGAATGGCTCCAGCAATTGCCATTAGTGTCAGCCCTCCAGCAATGACTCTGAAACCTGGTGCTTCTCGAAAGTTCTCTGTGAGTCTCACGGCTCGACGTTTGACTGGAACCTACAGTTTTGGAGAGGTTCTGTTGAAAGGTAGCCGGGGGCACAAGGTTAGGATCCCGGTGGTAGCTATGGGTTACCGACGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8 fn3_5

Protein Analysis

894

Amino Acids

95.8

Weight (kDa)

8.93

Isoelectric Point (pI)

30.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	102 - 204	4e-15	Peptidase inhibitor I9
Peptidase_S8	PF00082	231 - 717	9.8e-41	Subtilase family
fn3_5	PF06280	792 - 888	1.7e-16	Fn3-like domain
fn3_6	PF17766	796 - 888	3.7e-18	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002949)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19170 AT2G19170 AT4G30020 AT4G30020 AT4G30020 AT4G30020
fragaria_vesca	FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370
malus_domestica	MD13G1242900.v1.1 MD16G1249700.v1.1
prunus_persica	Prupe.1G076600_v2.0.a1 Prupe.1G076600_v2.0.a1 Prupe.1G076600_v2.0.a1 Prupe.1G076600_v2.0.a1
pyrus_communis	pycom13g21460 pycom16g20920 pycom16g20930
rosa_chinensis	RchiOBHm_Chr4g0399741 RchiOBHm_Chr4g0399751
rosa_laevigata	RLG00000009242
rosa_multiflora	Rmu_sc0005299.1_g000011 Rmu_sc0028740.1_g000001
rosa_roxburghii	Rroxscaffold_5G00344690
rosa_rugosa	Rorug04G0018500 Rorug04G0018600 Rorug04G0018700
rosa_samantha	Rh4AG096300 Rh4BG093700 Rh4CG105200 Rh4DG090100
rosa_wichuraiana	Rw4G007960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	335, 518, 2066
AasI	GACNNNNNNGTC	1 cut(s)	945
AccIII	TCCGGA	1 cut(s)	609
AciI	CCGC	7 cut(s)	26, 29, 1104, 1235, 1529, 1907, 2039
AclWI	GGATC	9 cut(s)	187, 761, 1667, 1737, 1917, 2231, 2249, 2647, 2660
AcoI	YGGCCR	4 cut(s)	23, 662, 1000, 2227
AcsI	RAATTY	5 cut(s)	981, 1007, 1667, 1689, 2386
AcuI	CTGAAG	2 cut(s)	227, 1449
AfaI	GTAC	6 cut(s)	763, 791, 799, 1427, 2308, 2434
AfiI	CCNNNNNNNGG	6 cut(s)	660, 671, 929, 1153, 1391, 1906
AflIII	ACRYGT	1 cut(s)	800
AgsI	TTSAA	8 cut(s)	155, 222, 365, 1198, 1561, 1792, 2392, 2626
AhlI	ACTAGT	1 cut(s)	404
AjiI	CACGTC	2 cut(s)	420, 2275
AjnI	CCWGG	9 cut(s)	1384, 1693, 1848, 1936, 1958, 2157, 2257, 2314, 2545
AjuI	GAANNNNNNNTTGG	2 cut(s)	815, 847
AleI	CACNNNNGTG	1 cut(s)	374
Alw21I	GWGCWC	1 cut(s)	574
Alw26I	GTCTC	3 cut(s)	1492, 1600, 2579
AlwI	GGATC	9 cut(s)	187, 761, 1667, 1737, 1917, 2231, 2249, 2647, 2660
Ama87I	CYCGRG	4 cut(s)	101, 545, 721, 1869
Aor13HI	TCCGGA	1 cut(s)	609
AoxI	GGCC	8 cut(s)	23, 662, 776, 1000, 1157, 2150, 2227, 2374
ApoI	RAATTY	5 cut(s)	981, 1007, 1667, 1689, 2386
ArsI	GACNNNNNNTTYG	4 cut(s)	1177, 1209, 2264, 2296
Asp700I	GAANNNNTTC	1 cut(s)	830
AspLEI	GCGC	1 cut(s)	2079
AspS9I	GGNCC	8 cut(s)	483, 607, 776, 1157, 1271, 1874, 1962, 2374
AsuC2I	CCSGG	5 cut(s)	576, 1659, 2141, 2636, 2657
AsuHPI	GGTGA	3 cut(s)	359, 944, 1392
AvaI	CYCGRG	4 cut(s)	101, 545, 721, 1869
AvaII	GGWCC	5 cut(s)	483, 607, 1271, 1874, 1962
BaeGI	GKGCMC	1 cut(s)	2643
BalI	TGGCCA	3 cut(s)	664, 1002, 2229
BamHI	GGATCC	1 cut(s)	2652
BanII	GRGCYC	2 cut(s)	859, 2093
Bbv12I	GWGCWC	1 cut(s)	574
BccI	CCATC	9 cut(s)	350, 923, 929, 1021, 1121, 1411, 1808, 2108, 2389
BceAI	ACGGC	4 cut(s)	16, 127, 242, 2595
BciT130I	CCWGG	9 cut(s)	1386, 1695, 1850, 1938, 1960, 2159, 2259, 2316, 2547
BclI	TGATCA	2 cut(s)	1111, 2481
BcnI	CCSGG	5 cut(s)	576, 1659, 2141, 2636, 2657
BcoDI	GTCTC	3 cut(s)	1492, 1600, 2579
BcuI	ACTAGT	1 cut(s)	404
BfaI	CTAG	3 cut(s)	405, 1931, 2252
BfmI	CTRYAG	6 cut(s)	14, 499, 954, 1797, 2133, 2602
BfoI	RGCGCY	1 cut(s)	2080
BglI	GCCNNNNNGGC	1 cut(s)	2495
BmcAI	AGTACT	1 cut(s)	763
Bme18I	GGWCC	5 cut(s)	483, 607, 1271, 1874, 1962
BmeT110I	CYCGRG	4 cut(s)	101, 545, 721, 1869
BmgBI	CACGTC	2 cut(s)	420, 2275
BmgT120I	GGNCC	8 cut(s)	483, 607, 776, 1157, 1271, 1874, 1962, 2374
BmrI	ACTGGG	1 cut(s)	44
BmsI	GCATC	6 cut(s)	520, 1198, 1589, 1812, 1894, 2314
BmuI	ACTGGG	1 cut(s)	44
BplI	GAGNNNNNCTC	2 cut(s)	2191, 2223
BpmI	CTGGAG	5 cut(s)	711, 1155, 2141, 2484, 2511
BpuEI	CTTGAG	2 cut(s)	458, 2448
BpuMI	CCSGG	5 cut(s)	576, 1659, 2141, 2636, 2657
BsaAI	YACGTR	1 cut(s)	2293
BsaBI	GATNNNNATC	2 cut(s)	1703, 1908
BsaI	GGTCTC	1 cut(s)	1492
BsaWI	WCCGGW	3 cut(s)	609, 653, 1338
BsaXI	ACNNNNNCTCC	8 cut(s)	236, 266, 1071, 1101, 1736, 1766, 2336, 2366
Bsc4I	CCNNNNNNNGG	6 cut(s)	660, 671, 929, 1153, 1391, 1906
Bse1I	ACTGG	4 cut(s)	50, 902, 1777, 2599
Bse3DI	GCAATG	2 cut(s)	95, 2538
Bse8I	GATNNNNATC	2 cut(s)	1703, 1908
BseAI	TCCGGA	1 cut(s)	609
BseBI	CCWGG	9 cut(s)	1386, 1695, 1850, 1938, 1960, 2159, 2259, 2316, 2547
BseGI	GGATG	6 cut(s)	739, 934, 1032, 1411, 1819, 2108
BseJI	GATNNNNATC	2 cut(s)	1703, 1908
BseLI	CCNNNNNNNGG	6 cut(s)	660, 671, 929, 1153, 1391, 1906
BseMI	GCAATG	2 cut(s)	95, 2538
BseMII	CTCAG	4 cut(s)	180, 224, 1133, 1500
BseNI	ACTGG	4 cut(s)	50, 902, 1777, 2599
BseSI	GKGCMC	1 cut(s)	2643
BseYI	CCCAGC	1 cut(s)	887
BsgI	GTGCAG	1 cut(s)	2019
BshFI	GGCC	8 cut(s)	25, 664, 778, 1002, 1159, 2152, 2229, 2376
BsiHKAI	GWGCWC	1 cut(s)	574
BsiHKCI	CYCGRG	4 cut(s)	101, 545, 721, 1869
BsiSI	CCGG	8 cut(s)	575, 610, 654, 1339, 1659, 2140, 2635, 2657
BslFI	GGGAC	2 cut(s)	496, 1948
BslI	CCNNNNNNNGG	6 cut(s)	660, 671, 929, 1153, 1391, 1906
BsmAI	GTCTC	3 cut(s)	1492, 1600, 2579
BsmFI	GGGAC	2 cut(s)	496, 1948
BsmI	GAATGC	2 cut(s)	471, 999
BsnI	GGCC	8 cut(s)	25, 664, 778, 1002, 1159, 2152, 2229, 2376
Bso31I	GGTCTC	1 cut(s)	1492
BsoBI	CYCGRG	4 cut(s)	101, 545, 721, 1869
Bsp1286I	GDGCHC	4 cut(s)	574, 859, 2093, 2643
Bsp13I	TCCGGA	1 cut(s)	609
Bsp1407I	TGTACA	1 cut(s)	2306
Bsp19I	CCATGG	2 cut(s)	20, 2370
BspACI	CCGC	7 cut(s)	26, 29, 1104, 1235, 1529, 1907, 2039
BspANI	GGCC	8 cut(s)	25, 664, 778, 1002, 1159, 2152, 2229, 2376
BspCNI	CTCAG	4 cut(s)	179, 225, 1134, 1499
BspEI	TCCGGA	1 cut(s)	609
BspMAI	CTGCAG	2 cut(s)	18, 958
BspPI	GGATC	9 cut(s)	187, 761, 1667, 1737, 1917, 2231, 2249, 2647, 2660
BspQI	GCTCTTC	1 cut(s)	821
BspTNI	GGTCTC	1 cut(s)	1492
BsrDI	GCAATG	2 cut(s)	95, 2538
BsrGI	TGTACA	1 cut(s)	2306
BsrI	ACTGG	4 cut(s)	50, 902, 1777, 2599
BssT1I	CCWWGG	5 cut(s)	20, 65, 1304, 2240, 2370
Bst2UI	CCWGG	9 cut(s)	1386, 1695, 1850, 1938, 1960, 2159, 2259, 2316, 2547
Bst6I	CTCTTC	2 cut(s)	284, 821
BstAPI	GCANNNNNTGC	1 cut(s)	962
BstAUI	TGTACA	1 cut(s)	2306
BstBAI	YACGTR	1 cut(s)	2293
BstC8I	GCNNGC	4 cut(s)	552, 570, 754, 1573
BstDEI	CTNAG	5 cut(s)	77, 166, 233, 1142, 1486
BstDSI	CCRYGG	3 cut(s)	20, 588, 2370
BstEII	GGTNACC	1 cut(s)	2672
BstF5I	GGATG	6 cut(s)	739, 934, 1032, 1411, 1819, 2108
BstH2I	RGCGCY	1 cut(s)	2080
BstHHI	GCGC	1 cut(s)	2079
BstMAI	GTCTC	3 cut(s)	1492, 1600, 2579
BstNI	CCWGG	9 cut(s)	1386, 1695, 1850, 1938, 1960, 2159, 2259, 2316, 2547
BstNSI	RCATGY	1 cut(s)	804
BstPI	GGTNACC	1 cut(s)	2672
BstSFI	CTRYAG	6 cut(s)	14, 499, 954, 1797, 2133, 2602
BstSLI	GKGCMC	1 cut(s)	2643
BstX2I	RGATCY	5 cut(s)	179, 1729, 1909, 2254, 2652
BstXI	CCANNNNNNTGG	2 cut(s)	376, 1010
BstYI	RGATCY	5 cut(s)	179, 1729, 1909, 2254, 2652
BsuRI	GGCC	8 cut(s)	25, 664, 778, 1002, 1159, 2152, 2229, 2376
BtgI	CCRYGG	3 cut(s)	20, 588, 2370
BtrI	CACGTC	2 cut(s)	420, 2275
BtsCI	GGATG	6 cut(s)	739, 934, 1032, 1411, 1819, 2108
BtsI	GCAGTG	2 cut(s)	369, 951
BtsIMutI	CAGTG	5 cut(s)	369, 951, 1430, 1707, 2454
Cac8I	GCNNGC	4 cut(s)	552, 570, 754, 1573
CfoI	GCGC	1 cut(s)	2079
Cfr13I	GGNCC	8 cut(s)	483, 607, 776, 1157, 1271, 1874, 1962, 2374
CsiI	ACCWGGT	2 cut(s)	2314, 2545
Csp6I	GTAC	6 cut(s)	762, 790, 798, 1426, 2307, 2433
CspCI	CAANNNNNGTGG	4 cut(s)	178, 213, 1003, 1038
CviQI	GTAC	6 cut(s)	762, 790, 798, 1426, 2307, 2433
DdeI	CTNAG	5 cut(s)	77, 166, 233, 1142, 1486
DraI	TTTAAA	1 cut(s)	1512
DrdI	GACNNNNNNGTC	1 cut(s)	945
DseDI	GACNNNNNNGTC	1 cut(s)	945
EaeI	YGGCCR	4 cut(s)	23, 662, 1000, 2227
Eam1104I	CTCTTC	2 cut(s)	284, 821
EarI	CTCTTC	2 cut(s)	284, 821
Eco130I	CCWWGG	5 cut(s)	20, 65, 1304, 2240, 2370
Eco147I	AGGCCT	1 cut(s)	2152
Eco24I	GRGCYC	2 cut(s)	859, 2093
Eco31I	GGTCTC	1 cut(s)	1492
Eco47I	GGWCC	5 cut(s)	483, 607, 1271, 1874, 1962
Eco57I	CTGAAG	2 cut(s)	227, 1449
Eco88I	CYCGRG	4 cut(s)	101, 545, 721, 1869
Eco91I	GGTNACC	1 cut(s)	2672
EcoO109I	RGGNCCY	3 cut(s)	483, 1271, 1874
EcoO65I	GGTNACC	1 cut(s)	2672
EcoRI	GAATTC	2 cut(s)	981, 1689
EcoRII	CCWGG	9 cut(s)	1384, 1693, 1848, 1936, 1958, 2157, 2257, 2314, 2545
EcoT14I	CCWWGG	5 cut(s)	20, 65, 1304, 2240, 2370
EcoT22I	ATGCAT	1 cut(s)	999
EcoT38I	GRGCYC	2 cut(s)	859, 2093
ErhI	CCWWGG	5 cut(s)	20, 65, 1304, 2240, 2370
FalI	AAGNNNNNCTT	4 cut(s)	815, 847, 2096, 2128
FaqI	GGGAC	2 cut(s)	496, 1948
FauI	CCCGC	2 cut(s)	1228, 1522
FbaI	TGATCA	2 cut(s)	1111, 2481
FokI	GGATG	6 cut(s)	726, 941, 1039, 1398, 1826, 2095
FriOI	GRGCYC	2 cut(s)	859, 2093
FspBI	CTAG	3 cut(s)	405, 1931, 2252
GlaI	GCGC	1 cut(s)	2078
GsaI	CCCAGC	1 cut(s)	891
GsuI	CTGGAG	5 cut(s)	711, 1155, 2141, 2484, 2511
HaeII	RGCGCY	1 cut(s)	2080
HaeIII	GGCC	8 cut(s)	25, 664, 778, 1002, 1159, 2152, 2229, 2376
HapII	CCGG	8 cut(s)	575, 610, 654, 1339, 1659, 2140, 2635, 2657
HhaI	GCGC	1 cut(s)	2079
Hin6I	GCGC	1 cut(s)	2077
HinP1I	GCGC	1 cut(s)	2077
HincII	GTYRAC	1 cut(s)	811
HindII	GTYRAC	1 cut(s)	811
HindIII	AAGCTT	2 cut(s)	133, 745
HinfI	GANTC	6 cut(s)	380, 410, 719, 1460, 2536, 2572
HpaII	CCGG	8 cut(s)	575, 610, 654, 1339, 1659, 2140, 2635, 2657
HphI	GGTGA	3 cut(s)	359, 944, 1392
Hpy166II	GTNNAC	5 cut(s)	83, 718, 811, 1428, 2307
Hpy188I	TCNGA	7 cut(s)	385, 992, 1489, 1600, 1947, 2176, 2541
Hpy8I	GTNNAC	5 cut(s)	83, 718, 811, 1428, 2307
Hpy99I	CGWCG	2 cut(s)	2589, 2682
HpyAV	CCTTC	4 cut(s)	314, 598, 1692, 2142
HpyCH4IV	ACGT	5 cut(s)	419, 1424, 2274, 2292, 2587
HpyF3I	CTNAG	5 cut(s)	77, 166, 233, 1142, 1486
HpySE526I	ACGT	5 cut(s)	419, 1424, 2274, 2292, 2587
HspAI	GCGC	1 cut(s)	2077
Kpn2I	TCCGGA	1 cut(s)	609
Ksp22I	TGATCA	2 cut(s)	1111, 2481
LguI	GCTCTTC	1 cut(s)	821
LmnI	GCTCC	6 cut(s)	577, 854, 1036, 1939, 2088, 2503
LweI	GCATC	6 cut(s)	520, 1198, 1589, 1812, 1894, 2314
MabI	ACCWGGT	2 cut(s)	2314, 2545
MaeI	CTAG	3 cut(s)	405, 1931, 2252
MaeII	ACGT	5 cut(s)	419, 1424, 2274, 2292, 2587
MaeIII	GTNAC	9 cut(s)	52, 310, 415, 836, 947, 1398, 2437, 2449, 2672
MboII	GAAGA	6 cut(s)	271, 838, 1455, 1532, 1904, 2480
MfeI	CAATTG	3 cut(s)	1107, 1623, 2505
MflI	RGATCY	5 cut(s)	179, 1729, 1909, 2254, 2652
MhlI	GDGCHC	4 cut(s)	574, 859, 2093, 2643
MlsI	TGGCCA	3 cut(s)	664, 1002, 2229
MluNI	TGGCCA	3 cut(s)	664, 1002, 2229
MlyI	GAGTC	4 cut(s)	389, 713, 2530, 2581
MmeI	TCCRAC	1 cut(s)	674
Mox20I	TGGCCA	3 cut(s)	664, 1002, 2229
Mph1103I	ATGCAT	1 cut(s)	999
MroI	TCCGGA	1 cut(s)	609
MroXI	GAANNNNTTC	1 cut(s)	830
MscI	TGGCCA	3 cut(s)	664, 1002, 2229
MseI	TTAA	6 cut(s)	897, 1511, 1716, 1782, 1884, 2190
MslI	CAYNNNNRTG	3 cut(s)	119, 374, 1002
Msp20I	TGGCCA	3 cut(s)	664, 1002, 2229
MspA1I	CMGCKG	4 cut(s)	887, 968, 1226, 1259
MspI	CCGG	8 cut(s)	575, 610, 654, 1339, 1659, 2140, 2635, 2657
MunI	CAATTG	3 cut(s)	1107, 1623, 2505
Mva1269I	GAATGC	2 cut(s)	471, 999
MvaI	CCWGG	9 cut(s)	1386, 1695, 1850, 1938, 1960, 2159, 2259, 2316, 2547
NciI	CCSGG	5 cut(s)	576, 1659, 2141, 2636, 2657
NcoI	CCATGG	2 cut(s)	20, 2370
NmuCI	GTSAC	5 cut(s)	310, 415, 947, 1398, 2449
NsiI	ATGCAT	1 cut(s)	999
NspI	RCATGY	1 cut(s)	804
OliI	CACNNNNGTG	1 cut(s)	374
PaeR7I	CTCGAG	1 cut(s)	1869
PceI	AGGCCT	1 cut(s)	2152
PciI	ACATGT	1 cut(s)	800
PciSI	GCTCTTC	1 cut(s)	821
PctI	GAATGC	2 cut(s)	471, 999
PdmI	GAANNNNTTC	1 cut(s)	830
PfeI	GAWTC	2 cut(s)	410, 1460
PfoI	TCCNGGA	1 cut(s)	2257
PleI	GAGTC	4 cut(s)	388, 713, 2530, 2580
PpsI	GAGTC	4 cut(s)	388, 713, 2530, 2580
Ppu21I	YACGTR	1 cut(s)	2293
PpuMI	RGGWCCY	3 cut(s)	483, 1271, 1874
PscI	ACATGT	1 cut(s)	800
PsiI	TTATAA	3 cut(s)	335, 518, 2066
Psp5II	RGGWCCY	3 cut(s)	483, 1271, 1874
Psp6I	CCWGG	9 cut(s)	1384, 1693, 1848, 1936, 1958, 2157, 2257, 2314, 2545
PspEI	GGTNACC	1 cut(s)	2672
PspFI	CCCAGC	1 cut(s)	887
PspGI	CCWGG	9 cut(s)	1384, 1693, 1848, 1936, 1958, 2157, 2257, 2314, 2545
PspPI	GGNCC	8 cut(s)	483, 607, 776, 1157, 1271, 1874, 1962, 2374
PspPPI	RGGWCCY	3 cut(s)	483, 1271, 1874
PspXI	VCTCGAGB	1 cut(s)	1869
PstI	CTGCAG	2 cut(s)	18, 958
PsuI	RGATCY	5 cut(s)	179, 1729, 1909, 2254, 2652
PvuII	CAGCTG	4 cut(s)	887, 968, 1226, 1259
RsaI	GTAC	6 cut(s)	763, 791, 799, 1427, 2308, 2434
RsaNI	GTAC	6 cut(s)	762, 790, 798, 1426, 2307, 2433
RseI	CAYNNNNRTG	3 cut(s)	119, 374, 1002
SapI	GCTCTTC	1 cut(s)	821
SaqAI	TTAA	6 cut(s)	897, 1511, 1716, 1782, 1884, 2190
Sau96I	GGNCC	8 cut(s)	483, 607, 776, 1157, 1271, 1874, 1962, 2374
ScaI	AGTACT	1 cut(s)	763
SchI	GAGTC	4 cut(s)	389, 713, 2530, 2581
SduI	GDGCHC	4 cut(s)	574, 859, 2093, 2643
SexAI	ACCWGGT	2 cut(s)	2314, 2545
SfaNI	GCATC	6 cut(s)	520, 1198, 1589, 1812, 1894, 2314
SfcI	CTRYAG	6 cut(s)	14, 499, 954, 1797, 2133, 2602
Sfr274I	CTCGAG	1 cut(s)	1869
SinI	GGWCC	5 cut(s)	483, 607, 1271, 1874, 1962
SlaI	CTCGAG	1 cut(s)	1869
SmiMI	CAYNNNNRTG	3 cut(s)	119, 374, 1002
SmlI	CTYRAG	3 cut(s)	437, 1869, 2463
SmoI	CTYRAG	3 cut(s)	437, 1869, 2463
SpeI	ACTAGT	1 cut(s)	404
SseBI	AGGCCT	1 cut(s)	2152
SsiI	CCGC	7 cut(s)	26, 29, 1104, 1235, 1529, 1907, 2039
SspI	AATATT	5 cut(s)	700, 1380, 1537, 1860, 1882
SspMI	CTAG	3 cut(s)	405, 1931, 2252
StuI	AGGCCT	1 cut(s)	2152
StyI	CCWWGG	5 cut(s)	20, 65, 1304, 2240, 2370
TaiI	ACGT	5 cut(s)	422, 1427, 2277, 2295, 2590
TaqI	TCGA	8 cut(s)	318, 396, 430, 1420, 1777, 1870, 2557, 2584
TatI	WGTACW	3 cut(s)	761, 789, 2306
TauI	GCSGC	3 cut(s)	28, 31, 1106
TfiI	GAWTC	2 cut(s)	410, 1460
Tru1I	TTAA	6 cut(s)	897, 1511, 1716, 1782, 1884, 2190
Tru9I	TTAA	6 cut(s)	897, 1511, 1716, 1782, 1884, 2190
TscAI	CASTG	5 cut(s)	376, 958, 1437, 1714, 2454
TseFI	GTSAC	5 cut(s)	310, 415, 947, 1398, 2449
Tsp45I	GTSAC	5 cut(s)	310, 415, 947, 1398, 2449
TspDTI	ATGAA	4 cut(s)	271, 402, 524, 1020
TspGWI	ACGGA	3 cut(s)	577, 855, 2388
TspRI	CASTG	5 cut(s)	376, 958, 1437, 1714, 2454
VpaK11BI	GGWCC	5 cut(s)	483, 607, 1271, 1874, 1962
XapI	RAATTY	5 cut(s)	981, 1007, 1667, 1689, 2386
XbaI	TCTAGA	1 cut(s)	2251
XceI	RCATGY	1 cut(s)	804
XcmI	CCANNNNNNNNNTGG	2 cut(s)	657, 1361
XhoI	CTCGAG	1 cut(s)	1869
XmnI	GAANNNNTTC	1 cut(s)	830
XspI	CTAG	3 cut(s)	405, 1931, 2252
ZrmI	AGTACT	1 cut(s)	763
Zsp2I	ATGCAT	1 cut(s)	999

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.