Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002949

Physical Location & Seq

Genomic Coordinates Forward (+)

27929359 .. 27936953

Gene Structure 7595 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1249700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2454 bp

ATGGTGGCCGTGGAGTTTGGGTGCACGGTTGCCGTACTGTTTTCCCTTTTAATTATTGGCAAAGCTGATGTTTACATCGTGACGATTGAAGGAGAGCCTATTATAAGTTATCAAGGTGGTGTTGATGGATTTGAAGCCACTGCTGTGGAATCTGATGAAAAGATTGATACAACTAGTGAATCAGTCACGTCCTATGCCCGACACTTGGAGAGTAAACATGATATGCTTCTTGGCATGTTGTTTGAGGAAGGGAGCTACCAAAAACTCTACAGCTATCAACATCTTATTAATGGATTTGCAGTCCACATTTCTCATGAACAGGCAGAAACATTGATGCGTGCTCCTGGTGTGAAATCGGTGGAGAGAGATTGGAAGGTGAAGAGACTTACAACCCACACCCCACAGTTTTTAGGTCTCCCAACTGGAGTATGGCCGACAGGAGGTGGCTTTGATAGGGCTGGGGAAGATATTGTGATAGGGTTTGTGGACTCAGGGATCTATCCGCACCATCCAAGCTTTGCGAGCCCCAATATCGATCCTTATGGGCCTGTTCCAAAGTATAGAGGTAAATGTGAAGTTGATCCAGACACAAAGAGGAACTTCTGTAATGGGAAGATTATTGGAGCTCACCATTTTGCCAAAGCTGCAATAGCAGCTGGGGTATTTAATCCTTCTATTGATTTTGACTCTCCTATGGATGGTGATGGACATGGAAGTCACACTGCAGCTATTGCAGCTGGTAATAATGGAATTCCAGTGAGAATGCGTGGTCATGAATTTGGGAAAGCAAGTGGGATGGCTCCCCGTGCCAGAATTGCTGTATATAAAGCCCTTTACAGGCTTTTTGGAGGATTTGTTGCAGATGTAGTTGCCGCAATTGATCAGGCTGTATATGATGGAGTGGATATATTGAGCCTTTCTGTGGGGCCAAACAGTCCTCCAGCTACCACCAAGACCACTTATTTGAATCCTTTTGATGCCACACTTCTTTCAGCTGTGAAAGCAGGTGTATTTGTTGCACAAGCAGCTGGAAATGGAGGACCTTTTCCAAAAACTTTAGTTTCTTATAGTCCTTGGATAGCTTCTGTTGCTGCTGCCATTGATGATCGCAGATACAAAAACCATTTAACGCTTGGAAATGGAAAACTATTAGCTGGACTTGGGCTGTCACCATCAACACATCCAAATCAAACGTACACACTGGTTGCTGCAAATGATGTGCTTCTTGATTCTTCTGTAGTTAAGTATAGCCCCTCAGACTGCCAGAAACCAGAAGTTTTAAACAAGAACTTGGTTCAGGGGAATATTCTTCTTTGTGGCTATTCATTCAATTTCGTTGTTGGTACTGCATCAATCAAGAAAGTGTCCGAAACAGCCAAAAGTCTTGGTGCAATTGGTTTTGTTCTTGTGGTGGAAAATGTTTCCCCAGGGACAAAATTTGATCCTGTTCCTGTAGGCGTTCCTGGGATTTTAATCACCGATGTTGAAAAGTCAATGGATCTTATAGAATATTACAACATCTCTACAGTAAGGGATTGGACTGGTCGTGTGAAGAGCTTCAAAGCTATAGGTAGCATTGGGAATGGTTTGACGCCTATACTCCACAAGTCTGCACCACAGGTGGCAATATTCTCTGCTAGAGGACCCAACATCAAAGATTTCAGCTACCAAGAAGCGGATCTTCTCAAGCCGGATATTCTAGCTCCTGGTTCTCTAATTTGGGCTGCTTGGTCTCCAAATGGAACAGATGAACCTGATTATGTTGGGGAGGGATTTGCCATGATTTCGGGAACAAGTATGGCTGCACCACATATAGCTGGAATAGCTGCTCTTATAAAGCAGAAGCACCCTCATTGGAGCCCTGCCGCCATCAAATCAGCTTTGATGACAACATCAACAACTATGGACAGGGCTGGCAGACCTCTTCAAGCACAGCAATATTCAGAAACACAAGCCATTAAGTTTGTCAGTGCCAACCCTTTTGATTATGGGAGTGGCCATGTCGATCCAAGAGCTGCTCTGGATCCTGGACTAATCTTTGATGCGGGCTATCAGGATTACTTGGGTTTCTTGTGTACAACACCTGGTATTAATTCTAATGAGATAAAGAAGTACACAAACTCCCCCTGCAATTACACTATGGGCCACCCGTCGAATTTCAACTCTCCTTCAATTACAGTAGCTCATCTTGTGAAAAGCCAAACAGTTACACGCACAGTCACAAATGTTGCACAAGAAGAAACCTATGTGATCACAACCAGAATGGCCCCAGCCGTTGCCATCGAAGCTAGCCCTCGGGCAATGACTCTGAGACCTGGTGCATCCCGAAAGTTCTCAGTGATTCTGACGGTTCGGTCTGTGACAGGATCCTACAGTTTTGGCGAGGTGATGATGAAAGGTAGCCGGGGGCACAAGGTGAGGATCGCTGTGGTAGCTATGGGATACCAACGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8 fn3_5

Protein Analysis

818

Amino Acids

87.44

Weight (kDa)

7.35

Isoelectric Point (pI)

28.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	25 - 127	4.3e-15	Peptidase inhibitor I9
Peptidase_S8	PF00082	154 - 635	1.4e-41	Subtilase family
fn3_5	PF06280	716 - 808	1.1e-14	Fn3-like domain
fn3_6	PF17766	719 - 811	2.7e-16	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002949)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19170 AT2G19170 AT4G30020 AT4G30020 AT4G30020 AT4G30020
fragaria_vesca	FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370
malus_domestica	MD13G1242900.v1.1 MD16G1249700.v1.1
prunus_persica	Prupe.1G076600_v2.0.a1 Prupe.1G076600_v2.0.a1 Prupe.1G076600_v2.0.a1 Prupe.1G076600_v2.0.a1
pyrus_communis	pycom13g21460 pycom16g20920 pycom16g20930
rosa_chinensis	RchiOBHm_Chr4g0399741 RchiOBHm_Chr4g0399751
rosa_laevigata	RLG00000009242
rosa_multiflora	Rmu_sc0005299.1_g000011 Rmu_sc0028740.1_g000001
rosa_roxburghii	Rroxscaffold_5G00344690
rosa_rugosa	Rorug04G0018500 Rorug04G0018600 Rorug04G0018700
rosa_samantha	Rh4AG096300 Rh4BG093700 Rh4CG105200 Rh4DG090100
rosa_wichuraiana	Rw4G007960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	104, 1835
AarI	CACCTGC	1 cut(s)	995
AasI	GACNNNNNNGTC	2 cut(s)	714, 2353
Acc36I	ACCTGC	1 cut(s)	995
AciI	CCGC	5 cut(s)	503, 873, 1676, 1866, 2045
AcoI	YGGCCR	3 cut(s)	6, 431, 1996
AcsI	RAATTY	4 cut(s)	750, 776, 1436, 2155
AcyI	GRCGYC	1 cut(s)	1592
AdeI	CACNNNGTG	1 cut(s)	2416
AfaI	GTAC	5 cut(s)	36, 1196, 1345, 2077, 2114
AfiI	CCNNNNNNNGG	6 cut(s)	205, 440, 698, 837, 922, 1675
AgsI	TTSAA	9 cut(s)	89, 134, 967, 1330, 1487, 1561, 1928, 2161, 2172
AhlI	ACTAGT	1 cut(s)	173
AjiI	CACGTC	1 cut(s)	189
AjnI	CCWGG	7 cut(s)	343, 1426, 1462, 1705, 2026, 2083, 2314
AleI	CACNNNNGTG	1 cut(s)	143
Alw21I	GWGCWC	3 cut(s)	26, 343, 628
Alw26I	GTCTC	4 cut(s)	376, 419, 1737, 2305
Alw44I	GTGCAC	1 cut(s)	22
Ama87I	CYCGRG	1 cut(s)	2295
AoxI	GGCC	7 cut(s)	6, 431, 545, 926, 1996, 2143, 2265
ApaLI	GTGCAC	1 cut(s)	22
ApoI	RAATTY	4 cut(s)	750, 776, 1436, 2155
ArsI	GACNNNNNNTTYG	2 cut(s)	946, 978
AseI	ATTAAT	2 cut(s)	288, 2091
Asp700I	GAANNNNTTC	1 cut(s)	1556
AspS9I	GGNCC	6 cut(s)	545, 926, 1040, 1643, 2143, 2266
AsuC2I	CCSGG	1 cut(s)	2405
AsuHPI	GGTGA	7 cut(s)	388, 620, 713, 1161, 1468, 2398, 2428
AsuNHI	GCTAGC	1 cut(s)	2288
AvaI	CYCGRG	1 cut(s)	2295
AvaII	GGWCC	2 cut(s)	1040, 1643
BaeGI	GKGCMC	2 cut(s)	26, 2412
BalI	TGGCCA	1 cut(s)	1998
BamHI	GGATCC	2 cut(s)	2023, 2366
BanII	GRGCYC	3 cut(s)	527, 628, 1862
Bbv12I	GWGCWC	3 cut(s)	26, 343, 628
BccI	CCATC	9 cut(s)	119, 516, 692, 698, 790, 890, 1180, 1877, 2288
BceAI	ACGGC	2 cut(s)	17, 2258
BciT130I	CCWGG	7 cut(s)	345, 1428, 1464, 1707, 2028, 2085, 2316
BciVI	GTATCC	1 cut(s)	2435
BclI	TGATCA	2 cut(s)	880, 2250
BcnI	CCSGG	1 cut(s)	2405
BcoDI	GTCTC	4 cut(s)	376, 419, 1737, 2305
BcuI	ACTAGT	1 cut(s)	173
BfaI	CTAG	4 cut(s)	174, 1638, 1700, 2289
BfmI	CTRYAG	7 cut(s)	268, 723, 1236, 1452, 1524, 1566, 2371
BfuAI	ACCTGC	1 cut(s)	995
BfuI	GTATCC	1 cut(s)	2435
Bme1390I	CCNGG	8 cut(s)	345, 1428, 1464, 1707, 2028, 2085, 2316, 2405
Bme18I	GGWCC	2 cut(s)	1040, 1643
BmeT110I	CYCGRG	1 cut(s)	2295
BmgBI	CACGTC	1 cut(s)	189
BmgT120I	GGNCC	6 cut(s)	545, 926, 1040, 1643, 2143, 2266
BmiI	GGNNCC	7 cut(s)	801, 927, 1645, 1859, 2025, 2268, 2368
BmrFI	CCNGG	8 cut(s)	345, 1428, 1464, 1707, 2028, 2085, 2316, 2405
BmsI	GCATC	5 cut(s)	324, 967, 1358, 2032, 2330
BmtI	GCTAGC	1 cut(s)	2292
BpmI	CTGGAG	2 cut(s)	444, 924
BpuEI	CTTGAG	1 cut(s)	1670
BpuMI	CCSGG	1 cut(s)	2405
Bsa29I	ATCGAT	1 cut(s)	534
BsaBI	GATNNNNATC	1 cut(s)	1472
BsaHI	GRCGYC	1 cut(s)	1592
BsaI	GGTCTC	3 cut(s)	419, 1737, 2305
BsaJI	CCNNGG	7 cut(s)	9, 1073, 1426, 1427, 1463, 2294, 2404
BsaXI	ACNNNNNCTCC	6 cut(s)	5, 35, 840, 870, 2105, 2135
Bsc4I	CCNNNNNNNGG	6 cut(s)	205, 440, 698, 837, 922, 1675
Bse1I	ACTGG	4 cut(s)	427, 755, 1206, 1546
Bse3DI	GCAATG	1 cut(s)	2307
Bse8I	GATNNNNATC	1 cut(s)	1472
BseBI	CCWGG	7 cut(s)	345, 1428, 1464, 1707, 2028, 2085, 2316
BseCI	ATCGAT	1 cut(s)	534
BseDI	CCNNGG	7 cut(s)	9, 1073, 1426, 1427, 1463, 2294, 2404
BseGI	GGATG	5 cut(s)	508, 703, 801, 1180, 2321
BseJI	GATNNNNATC	1 cut(s)	1472
BseLI	CCNNNNNNNGG	6 cut(s)	205, 440, 698, 837, 922, 1675
BseMI	GCAATG	1 cut(s)	2307
BseMII	CTCAG	4 cut(s)	504, 1269, 2300, 2349
BseNI	ACTGG	4 cut(s)	427, 755, 1206, 1546
BseSI	GKGCMC	2 cut(s)	26, 2412
BseYI	CCCAGC	3 cut(s)	458, 656, 2269
BsgI	GTGCAG	2 cut(s)	1596, 1788
BshFI	GGCC	7 cut(s)	8, 433, 547, 928, 1998, 2145, 2267
BshVI	ATCGAT	1 cut(s)	534
BsiHKAI	GWGCWC	3 cut(s)	26, 343, 628
BsiHKCI	CYCGRG	1 cut(s)	2295
BsiSI	CCGG	2 cut(s)	1691, 2404
BslFI	GGGAC	1 cut(s)	1444
BslI	CCNNNNNNNGG	6 cut(s)	205, 440, 698, 837, 922, 1675
BsmAI	GTCTC	4 cut(s)	376, 419, 1737, 2305
BsmFI	GGGAC	1 cut(s)	1444
BsmI	GAATGC	1 cut(s)	768
BsnI	GGCC	7 cut(s)	8, 433, 547, 928, 1998, 2145, 2267
Bso31I	GGTCTC	3 cut(s)	419, 1737, 2305
BsoBI	CYCGRG	1 cut(s)	2295
Bsp1286I	GDGCHC	6 cut(s)	26, 343, 527, 628, 1862, 2412
Bsp1407I	TGTACA	1 cut(s)	2075
BspACI	CCGC	5 cut(s)	503, 873, 1676, 1866, 2045
BspANI	GGCC	7 cut(s)	8, 433, 547, 928, 1998, 2145, 2267
BspCNI	CTCAG	4 cut(s)	503, 1268, 2301, 2348
BspDI	ATCGAT	1 cut(s)	534
BspHI	TCATGA	2 cut(s)	313, 772
BspLI	GGNNCC	7 cut(s)	801, 927, 1645, 1859, 2025, 2268, 2368
BspMAI	CTGCAG	1 cut(s)	727
BspMI	ACCTGC	1 cut(s)	995
BspOI	GCTAGC	1 cut(s)	2292
BspQI	GCTCTTC	1 cut(s)	1547
BspTNI	GGTCTC	3 cut(s)	419, 1737, 2305
BsrDI	GCAATG	1 cut(s)	2307
BsrGI	TGTACA	1 cut(s)	2075
BsrI	ACTGG	4 cut(s)	427, 755, 1206, 1546
BssECI	CCNNGG	7 cut(s)	9, 1073, 1426, 1427, 1463, 2294, 2404
BssNI	GRCGYC	1 cut(s)	1592
BssT1I	CCWWGG	1 cut(s)	1073
Bst2UI	CCWGG	7 cut(s)	345, 1428, 1464, 1707, 2028, 2085, 2316
Bst6I	CTCTTC	3 cut(s)	374, 1547, 1929
BstACI	GRCGYC	1 cut(s)	1592
BstAPI	GCANNNNNTGC	1 cut(s)	731
BstAUI	TGTACA	1 cut(s)	2075
BstC8I	GCNNGC	5 cut(s)	339, 523, 1915, 2047, 2290
BstDEI	CTNAG	4 cut(s)	490, 1255, 2309, 2335
BstDSI	CCRYGG	1 cut(s)	9
BstF5I	GGATG	5 cut(s)	508, 703, 801, 1180, 2321
BstMAI	GTCTC	4 cut(s)	376, 419, 1737, 2305
BstNI	CCWGG	7 cut(s)	345, 1428, 1464, 1707, 2028, 2085, 2316
BstNSI	RCATGY	1 cut(s)	238
BstSCI	CCNGG	8 cut(s)	343, 1426, 1462, 1705, 2026, 2083, 2314, 2403
BstSFI	CTRYAG	7 cut(s)	268, 723, 1236, 1452, 1524, 1566, 2371
BstSLI	GKGCMC	2 cut(s)	26, 2412
BstX2I	RGATCY	5 cut(s)	495, 1498, 1678, 2023, 2366
BstXI	CCANNNNNNTGG	1 cut(s)	145
BstYI	RGATCY	5 cut(s)	495, 1498, 1678, 2023, 2366
Bsu15I	ATCGAT	1 cut(s)	534
BsuI	GTATCC	1 cut(s)	2435
BsuRI	GGCC	7 cut(s)	8, 433, 547, 928, 1998, 2145, 2267
BsuTUI	ATCGAT	1 cut(s)	534
BtgI	CCRYGG	1 cut(s)	9
BtrI	CACGTC	1 cut(s)	189
BtsCI	GGATG	5 cut(s)	508, 703, 801, 1180, 2321
BtsI	GCAGTG	2 cut(s)	138, 720
BtsIMutI	CAGTG	6 cut(s)	138, 720, 762, 1199, 1975, 2343
BveI	ACCTGC	1 cut(s)	995
Cac8I	GCNNGC	5 cut(s)	339, 523, 1915, 2047, 2290
CciI	TCATGA	2 cut(s)	313, 772
Cfr13I	GGNCC	6 cut(s)	545, 926, 1040, 1643, 2143, 2266
ClaI	ATCGAT	1 cut(s)	534
CseI	GACGC	1 cut(s)	1600
CsiI	ACCWGGT	2 cut(s)	2083, 2314
Csp6I	GTAC	5 cut(s)	35, 1195, 1344, 2076, 2113
CviAII	CATG	7 cut(s)	218, 235, 314, 710, 773, 1780, 2000
CviQI	GTAC	5 cut(s)	35, 1195, 1344, 2076, 2113
DdeI	CTNAG	4 cut(s)	490, 1255, 2309, 2335
DraI	TTTAAA	1 cut(s)	1281
DraIII	CACNNNGTG	1 cut(s)	2416
DrdI	GACNNNNNNGTC	2 cut(s)	714, 2353
DseDI	GACNNNNNNGTC	2 cut(s)	714, 2353
EaeI	YGGCCR	3 cut(s)	6, 431, 1996
Eam1104I	CTCTTC	3 cut(s)	374, 1547, 1929
EarI	CTCTTC	3 cut(s)	374, 1547, 1929
Ecl136II	GAGCTC	1 cut(s)	626
Eco130I	CCWWGG	1 cut(s)	1073
Eco24I	GRGCYC	3 cut(s)	527, 628, 1862
Eco31I	GGTCTC	3 cut(s)	419, 1737, 2305
Eco47I	GGWCC	2 cut(s)	1040, 1643
Eco53kI	GAGCTC	1 cut(s)	626
Eco88I	CYCGRG	1 cut(s)	2295
EcoICRI	GAGCTC	1 cut(s)	626
EcoO109I	RGGNCCY	2 cut(s)	1040, 1643
EcoRI	GAATTC	1 cut(s)	750
EcoRII	CCWGG	7 cut(s)	343, 1426, 1462, 1705, 2026, 2083, 2314
EcoT14I	CCWWGG	1 cut(s)	1073
EcoT38I	GRGCYC	3 cut(s)	527, 628, 1862
ErhI	CCWWGG	1 cut(s)	1073
FaeI	CATG	7 cut(s)	221, 238, 317, 713, 776, 1783, 2003
FalI	AAGNNNNNCTT	4 cut(s)	584, 616, 1665, 1697
FaqI	GGGAC	1 cut(s)	1444
FatI	CATG	7 cut(s)	217, 234, 313, 709, 772, 1779, 1999
FauI	CCCGC	1 cut(s)	2038
FbaI	TGATCA	2 cut(s)	880, 2250
FokI	GGATG	5 cut(s)	495, 710, 808, 1167, 2308
FriOI	GRGCYC	3 cut(s)	527, 628, 1862
FspBI	CTAG	4 cut(s)	174, 1638, 1700, 2289
GsaI	CCCAGC	3 cut(s)	462, 660, 2273
GsuI	CTGGAG	2 cut(s)	444, 924
HaeIII	GGCC	7 cut(s)	8, 433, 547, 928, 1998, 2145, 2267
HapII	CCGG	2 cut(s)	1691, 2404
HgaI	GACGC	1 cut(s)	1600
Hin1I	GRCGYC	1 cut(s)	1592
Hin1II	CATG	7 cut(s)	221, 238, 317, 713, 776, 1783, 2003
HindIII	AAGCTT	1 cut(s)	514
HinfI	GANTC	8 cut(s)	149, 179, 488, 686, 967, 1229, 2305, 2341
HpaII	CCGG	2 cut(s)	1691, 2404
HphI	GGTGA	7 cut(s)	388, 620, 713, 1161, 1468, 2398, 2428
Hpy166II	GTNNAC	8 cut(s)	24, 73, 215, 304, 487, 1197, 2076, 2115
Hpy188I	TCNGA	6 cut(s)	154, 1258, 1369, 1945, 2310, 2346
Hpy8I	GTNNAC	8 cut(s)	24, 73, 215, 304, 487, 1197, 2076, 2115
Hpy99I	CGWCG	1 cut(s)	2155
HpyAV	CCTTC	5 cut(s)	83, 242, 367, 681, 2178
HpyCH4IV	ACGT	2 cut(s)	188, 1193
HpyF3I	CTNAG	4 cut(s)	490, 1255, 2309, 2335
HpySE526I	ACGT	2 cut(s)	188, 1193
Hsp92I	GRCGYC	1 cut(s)	1592
Hsp92II	CATG	7 cut(s)	221, 238, 317, 713, 776, 1783, 2003
Ksp22I	TGATCA	2 cut(s)	880, 2250
LguI	GCTCTTC	1 cut(s)	1547
LmnI	GCTCC	6 cut(s)	252, 346, 623, 805, 1708, 1857
LweI	GCATC	5 cut(s)	324, 967, 1358, 2032, 2330
MabI	ACCWGGT	2 cut(s)	2083, 2314
MaeI	CTAG	4 cut(s)	174, 1638, 1700, 2289
MaeII	ACGT	2 cut(s)	188, 1193
MaeIII	GTNAC	7 cut(s)	79, 184, 716, 1167, 2206, 2218, 2359
MboII	GAAGA	9 cut(s)	391, 476, 625, 1224, 1301, 1564, 1673, 1916, 2249
MfeI	CAATTG	2 cut(s)	876, 1392
MflI	RGATCY	5 cut(s)	495, 1498, 1678, 2023, 2366
MhlI	GDGCHC	6 cut(s)	26, 343, 527, 628, 1862, 2412
MlsI	TGGCCA	1 cut(s)	1998
MluNI	TGGCCA	1 cut(s)	1998
MlyI	GAGTC	3 cut(s)	482, 680, 2299
Mox20I	TGGCCA	1 cut(s)	1998
MroXI	GAANNNNTTC	1 cut(s)	1556
MscI	TGGCCA	1 cut(s)	1998
MseI	TTAA	9 cut(s)	50, 288, 666, 1127, 1242, 1280, 1472, 1959, 2091
MslI	CAYNNNNRTG	1 cut(s)	143
Msp20I	TGGCCA	1 cut(s)	1998
MspA1I	CMGCKG	4 cut(s)	656, 737, 995, 1028
MspI	CCGG	2 cut(s)	1691, 2404
MspR9I	CCNGG	8 cut(s)	345, 1428, 1464, 1707, 2028, 2085, 2316, 2405
MunI	CAATTG	2 cut(s)	876, 1392
Mva1269I	GAATGC	1 cut(s)	768
MvaI	CCWGG	7 cut(s)	345, 1428, 1464, 1707, 2028, 2085, 2316
NciI	CCSGG	1 cut(s)	2405
NheI	GCTAGC	1 cut(s)	2288
NlaIII	CATG	7 cut(s)	221, 238, 317, 713, 776, 1783, 2003
NlaIV	GGNNCC	7 cut(s)	801, 927, 1645, 1859, 2025, 2268, 2368
NmuCI	GTSAC	6 cut(s)	79, 184, 716, 1167, 2218, 2359
NspI	RCATGY	1 cut(s)	238
OliI	CACNNNNGTG	1 cut(s)	143
PagI	TCATGA	2 cut(s)	313, 772
PaqCI	CACCTGC	1 cut(s)	995
PasI	CCCWGGG	1 cut(s)	1427
PciSI	GCTCTTC	1 cut(s)	1547
PctI	GAATGC	1 cut(s)	768
PdmI	GAANNNNTTC	1 cut(s)	1556
PfeI	GAWTC	5 cut(s)	149, 179, 967, 1229, 2341
PfoI	TCCNGGA	1 cut(s)	2026
PleI	GAGTC	3 cut(s)	482, 680, 2299
PpsI	GAGTC	3 cut(s)	482, 680, 2299
PpuMI	RGGWCCY	2 cut(s)	1040, 1643
PshBI	ATTAAT	2 cut(s)	288, 2091
PsiI	TTATAA	2 cut(s)	104, 1835
Psp124BI	GAGCTC	1 cut(s)	628
Psp5II	RGGWCCY	2 cut(s)	1040, 1643
Psp6I	CCWGG	7 cut(s)	343, 1426, 1462, 1705, 2026, 2083, 2314
PspFI	CCCAGC	3 cut(s)	458, 656, 2269
PspGI	CCWGG	7 cut(s)	343, 1426, 1462, 1705, 2026, 2083, 2314
PspN4I	GGNNCC	7 cut(s)	801, 927, 1645, 1859, 2025, 2268, 2368
PspPI	GGNCC	6 cut(s)	545, 926, 1040, 1643, 2143, 2266
PspPPI	RGGWCCY	2 cut(s)	1040, 1643
PstI	CTGCAG	1 cut(s)	727
PsuI	RGATCY	5 cut(s)	495, 1498, 1678, 2023, 2366
PvuII	CAGCTG	4 cut(s)	656, 737, 995, 1028
RsaI	GTAC	5 cut(s)	36, 1196, 1345, 2077, 2114
RsaNI	GTAC	5 cut(s)	35, 1195, 1344, 2076, 2113
RseI	CAYNNNNRTG	1 cut(s)	143
SacI	GAGCTC	1 cut(s)	628
SapI	GCTCTTC	1 cut(s)	1547
SaqAI	TTAA	9 cut(s)	50, 288, 666, 1127, 1242, 1280, 1472, 1959, 2091
Sau96I	GGNCC	6 cut(s)	545, 926, 1040, 1643, 2143, 2266
SchI	GAGTC	3 cut(s)	482, 680, 2299
ScrFI	CCNGG	8 cut(s)	345, 1428, 1464, 1707, 2028, 2085, 2316, 2405
SduI	GDGCHC	6 cut(s)	26, 343, 527, 628, 1862, 2412
SexAI	ACCWGGT	2 cut(s)	2083, 2314
SfaNI	GCATC	5 cut(s)	324, 967, 1358, 2032, 2330
SfcI	CTRYAG	7 cut(s)	268, 723, 1236, 1452, 1524, 1566, 2371
SinI	GGWCC	2 cut(s)	1040, 1643
SmiMI	CAYNNNNRTG	1 cut(s)	143
SmlI	CTYRAG	1 cut(s)	1685
SmoI	CTYRAG	1 cut(s)	1685
SpeI	ACTAGT	1 cut(s)	173
SsiI	CCGC	5 cut(s)	503, 873, 1676, 1866, 2045
SspI	AATATT	4 cut(s)	1306, 1511, 1629, 1940
SspMI	CTAG	4 cut(s)	174, 1638, 1700, 2289
SstI	GAGCTC	1 cut(s)	628
StyD4I	CCNGG	8 cut(s)	343, 1426, 1462, 1705, 2026, 2083, 2314, 2403
StyI	CCWWGG	1 cut(s)	1073
TaiI	ACGT	2 cut(s)	191, 1196
TaqI	TCGA	4 cut(s)	534, 2004, 2153, 2283
TaqII	GACCGA	1 cut(s)	2343
TatI	WGTACW	2 cut(s)	2075, 2112
TauI	GCSGC	2 cut(s)	875, 1868
TfiI	GAWTC	5 cut(s)	149, 179, 967, 1229, 2341
Tru1I	TTAA	9 cut(s)	50, 288, 666, 1127, 1242, 1280, 1472, 1959, 2091
Tru9I	TTAA	9 cut(s)	50, 288, 666, 1127, 1242, 1280, 1472, 1959, 2091
TscAI	CASTG	6 cut(s)	145, 727, 762, 1206, 1975, 2343
TseFI	GTSAC	6 cut(s)	79, 184, 716, 1167, 2218, 2359
Tsp45I	GTSAC	6 cut(s)	79, 184, 716, 1167, 2218, 2359
TspDTI	ATGAA	6 cut(s)	171, 330, 789, 1314, 1764, 2408
TspRI	CASTG	6 cut(s)	145, 727, 762, 1206, 1975, 2343
VneI	GTGCAC	1 cut(s)	22
VpaK11BI	GGWCC	2 cut(s)	1040, 1643
VspI	ATTAAT	2 cut(s)	288, 2091
XapI	RAATTY	4 cut(s)	750, 776, 1436, 2155
XceI	RCATGY	1 cut(s)	238
XcmI	CCANNNNNNNNNTGG	2 cut(s)	426, 1130
XmnI	GAANNNNTTC	1 cut(s)	1556
XspI	CTAG	4 cut(s)	174, 1638, 1700, 2289

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.