Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0002949

Physical Location & Seq

Genomic Coordinates Reverse (-)

49624082 .. 49634936

Gene Structure 10855 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000009242

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2460 bp

ATGAAAGTTGTTGATTATGCACACGTTTCCACCCCAGCCATTTCTGGATCTCTCTCTGTACAAATCTTGTGGAGTACTGAAGTTGCATACTTCAATGGAGAGCCTATTATAAGTTATAGAGGTGATGTTGATGGTTTTGAAGCCACTGCTGTGGAATCTGATGAAAAGATCGACACCACTAGTGAATCAGTCACGTCCTATGCTCGACACCTTGAGAATAAACATGATATGCTTCTTGGAATGCTGTTTGAGCAAGGGACCTACAAGAAACTCTACAGCTATCAGCATCTTATAAATGGCTTTGCAGTTCATATTTCCCCTGAGCAGGCAGAAAGACTAATGCGTGCTCCGGGTGTGAAATCCGTGGAGAGAGATTGGAAGGTCCGGAGGCTTACAACTCACACACCACAGTTTTTAGGGCTTCCAACCGGAGTATGGCCAACAGGAGGTGGCTTTGATAGGGCTGGAGAAAATATTGTGATAGGGTTTGTGGATTCGGGGATTTACCCTCATCATCCAAGCTTTGCAAGCCACAGTACTGATCCGTACGGGCCTGTTCCCAAGTACAGAGGTACATGTGAAGTTGACCCAGACACCAAGAAGAGCTTCTGTAACGGAAAGATTATTGGAGCCCACCATTTCGCCAAAGCTGCAATAGCAGCTGGGGTGTTTAATCCAGTTATTGATTTCGCCTCTCCTATGGATGGTGATGGACATGGAAGTCACACTGCAGCTATTGCAGCTGGAAACAATGGAATTCCTGTCAGAATGCATGGCCATGAATTTGGCAAAGCAAGTGGGATGGCTCCCCGTGCCAGAATTGCTGTATATAAAGCTCTTTACAGGCTCTTTGGAGGGTTTGTTGCAGATGTAGTAGCCGCAATTGATCAGGCTGTTTATGATGGAGTGGATATACTTAGCCTTTCAGTGGGGCCAAACAGTCCTCCAGCTACCACCAAGACCACTTATTTGAACCCGTTTGATGCCACACTTCTTTCAGCTGTGAAAGCTGGTGTATTTGTTGCACAGGCAGCTGGAAATGGAGGACCTTTTCCAAAAACTTTGGTTTCTTATAGCCCTTGGATAGCTTCTGTTGCTGCTGCCATAGATGACCGGAGATACAAAAACCATTTCACGCTTGGGAATGGAAAAATATTACCTGGACTTGGGTTGTCACCATCTACACTTCCAAATCGAACGTACACACTGGTTGCTGCAAATGATGTGCTTTTGGATTCTTCAGTAGTGAAGTATAGCCCCTCAGACTGCCAGAGACCAGAAGTTTTAAACAAGAACTTGGTTGAGGGGAATATTCTTCTTTGTGGCTATTCGTTCAATTTTGTTGCTGGCTCTGCATCAATCAAGAAAGTCTCGGAAACAGCTAAAAGTCTTGGTGCAATTGGCTTTGTTCTTGCTGTGGAAAATGTTTCCCCAGGAACCAAATTTGATCCTGTTCCTTCTGGAATTCCTGGGATTGTAATCACTGATGTTAAGAAGTCAATGGATCTTATAGATTACTACAACATCTCCACATCAAGAGATTGGACTGGTCGAGTTAAGAGTTTCAAAGCTATAGGTAGCATTGGGGATGGTTTGACGCCTATACTCCACAAATCAGCACCCCAGGTGGCAATATTCTCTGCTCGAGGACCCAATATTAAAGATTTCAGCTTCCAAGATGCGGATCTTCTCAAACCAGATATTCTAGCTCCTGGTTCTCTGATTTGGGCTGCCTGGTCCCCTAATGGAACTGATGAGCCTGATTATGTTGGGGAGGGATTTGCCATGATTTCTGGAACAAGTATGGCTGCACCGCATATAGCAGGAATAGCTGCTCTTATAAAGCAGAAGCACCCTCATTGGAGCCCTGCTGCCATCAAGTCATCCTTGATGACAACATCAACAACTATAGACCGGGCTGGAAGGCCTCTCCAGGCACAACAGACTTCCGAAACACAAGGCATTAAGTTTGTGAGTGCTACTCCTTTTGATTATGGTAGTGGCCATGTTGATCCAAGGGCTGCTCTAGATCCTGGACTAATCTTTGACGTGGGTTTTGAGGATTACGTGGGCTTCTTGTGTACAACACCTGGTATTGATGCCCATGAGATAAAGAAATACACAAACTCCCCCTGCAATTACACCATGGGCCACCCATCAAATTTCAACTCTCCGTCAATCACAGTATCTCATCTTGTAGGAAGTCGTACAGTAACTCGCACAGTGACAAATGTTGCTCAAGAAGAAACCTATGTGATGACAGCCAGAATGGCTCCAGCAATTGCCATTAGTGTCAGCCCTCCAGCAATGACCCTGAAACCTGGTGCTTCTAGAAAGTTCTCTGTGAGTCTCACAGCTCGACGTTTGACTGGAACCTACAGTTTTGGAGAGGTTCTGTTGAAAGGTAGCCGGGGGCACAAGGTTAGGATCCCGGTGGTAGCTATGGGCTACCGACGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8 fn3_5

Protein Analysis

820

Amino Acids

87.58

Weight (kDa)

8.56

Isoelectric Point (pI)

29.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	67 - 129	1.6e-14	Peptidase inhibitor I9
Peptidase_S8	PF00082	156 - 642	3.8e-41	Subtilase family
fn3_5	PF06280	716 - 813	8.3e-17	Fn3-like domain
fn3_6	PF17766	721 - 813	2.2e-18	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002949)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19170 AT2G19170 AT4G30020 AT4G30020 AT4G30020 AT4G30020
fragaria_vesca	FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370
malus_domestica	MD13G1242900.v1.1 MD16G1249700.v1.1
prunus_persica	Prupe.1G076600_v2.0.a1 Prupe.1G076600_v2.0.a1 Prupe.1G076600_v2.0.a1 Prupe.1G076600_v2.0.a1
pyrus_communis	pycom13g21460 pycom16g20920 pycom16g20930
rosa_chinensis	RchiOBHm_Chr4g0399741 RchiOBHm_Chr4g0399751
rosa_laevigata	RLG00000009242
rosa_multiflora	Rmu_sc0005299.1_g000011 Rmu_sc0028740.1_g000001
rosa_roxburghii	Rroxscaffold_5G00344690
rosa_rugosa	Rorug04G0018500 Rorug04G0018600 Rorug04G0018700
rosa_samantha	Rh4AG096300 Rh4BG093700 Rh4CG105200 Rh4DG090100
rosa_wichuraiana	Rw4G007960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	110, 293, 1841
AasI	GACNNNNNNGTC	1 cut(s)	720
AccIII	TCCGGA	1 cut(s)	384
AciI	CCGC	3 cut(s)	879, 1682, 1814
AclWI	GGATC	9 cut(s)	55, 536, 1442, 1512, 1692, 2006, 2024, 2422, 2435
AcoI	YGGCCR	3 cut(s)	437, 775, 2002
AcsI	RAATTY	5 cut(s)	756, 782, 1442, 1464, 2161
AcuI	CTGAAG	2 cut(s)	99, 1224
AcyI	GRCGYC	1 cut(s)	1598
AfaI	GTAC	9 cut(s)	60, 76, 538, 548, 566, 574, 1202, 2083, 2209
AfiI	CCNNNNNNNGG	7 cut(s)	325, 435, 446, 704, 928, 1166, 1681
AflIII	ACRYGT	2 cut(s)	22, 575
AgsI	TTSAA	7 cut(s)	94, 140, 973, 1336, 1567, 2167, 2401
AhlI	ACTAGT	1 cut(s)	179
AjiI	CACGTC	2 cut(s)	195, 2050
AjuI	GAANNNNNNNTTGG	2 cut(s)	590, 622
AleI	CACNNNNGTG	1 cut(s)	149
Alw21I	GWGCWC	1 cut(s)	349
Alw26I	GTCTC	3 cut(s)	1267, 1375, 2354
AlwI	GGATC	9 cut(s)	55, 536, 1442, 1512, 1692, 2006, 2024, 2422, 2435
Ama87I	CYCGRG	1 cut(s)	1644
Aor13HI	TCCGGA	1 cut(s)	384
AoxI	GGCC	7 cut(s)	437, 551, 775, 932, 1925, 2002, 2149
ApoI	RAATTY	5 cut(s)	756, 782, 1442, 1464, 2161
ArsI	GACNNNNNNTTYG	4 cut(s)	952, 984, 2039, 2071
Asp700I	GAANNNNTTC	1 cut(s)	605
AspS9I	GGNCC	8 cut(s)	258, 382, 551, 932, 1046, 1649, 1737, 2149
AsuC2I	CCSGG	4 cut(s)	351, 1916, 2411, 2432
AsuHPI	GGTGA	3 cut(s)	134, 719, 1167
AvaI	CYCGRG	1 cut(s)	1644
AvaII	GGWCC	5 cut(s)	258, 382, 1046, 1649, 1737
BaeGI	GKGCMC	1 cut(s)	2418
BalI	TGGCCA	3 cut(s)	439, 777, 2004
BamHI	GGATCC	1 cut(s)	2427
BanII	GRGCYC	2 cut(s)	634, 1868
Bbv12I	GWGCWC	1 cut(s)	349
BccI	CCATC	9 cut(s)	125, 698, 704, 796, 896, 1186, 1583, 1883, 2164
BclI	TGATCA	1 cut(s)	886
BcnI	CCSGG	4 cut(s)	351, 1916, 2411, 2432
BcoDI	GTCTC	3 cut(s)	1267, 1375, 2354
BcuI	ACTAGT	1 cut(s)	179
BfaI	CTAG	4 cut(s)	180, 1706, 2027, 2331
BfmI	CTRYAG	5 cut(s)	274, 729, 1572, 1908, 2377
BglI	GCCNNNNNGGC	1 cut(s)	2270
BmcAI	AGTACT	2 cut(s)	76, 538
Bme18I	GGWCC	5 cut(s)	258, 382, 1046, 1649, 1737
BmeT110I	CYCGRG	1 cut(s)	1644
BmgBI	CACGTC	2 cut(s)	195, 2050
BmgT120I	GGNCC	8 cut(s)	258, 382, 551, 932, 1046, 1649, 1737, 2149
BmsI	GCATC	5 cut(s)	295, 973, 1364, 1669, 2089
BplI	GAGNNNNNCTC	2 cut(s)	1966, 1998
BpmI	CTGGAG	5 cut(s)	486, 930, 1916, 2259, 2286
Bpu10I	CCTNAGC	1 cut(s)	321
BpuEI	CTTGAG	2 cut(s)	233, 2223
BpuMI	CCSGG	4 cut(s)	351, 1916, 2411, 2432
BsaAI	YACGTR	1 cut(s)	2068
BsaBI	GATNNNNATC	2 cut(s)	1478, 1683
BsaHI	GRCGYC	1 cut(s)	1598
BsaI	GGTCTC	1 cut(s)	1267
BsaJI	CCNNGG	8 cut(s)	363, 1079, 1432, 1469, 1623, 2015, 2145, 2410
BsaWI	WCCGGW	3 cut(s)	384, 428, 1113
BsaXI	ACNNNNNCTCC	6 cut(s)	846, 876, 1511, 1541, 2111, 2141
Bsc4I	CCNNNNNNNGG	7 cut(s)	325, 435, 446, 704, 928, 1166, 1681
Bse1I	ACTGG	4 cut(s)	677, 1212, 1552, 2374
Bse3DI	GCAATG	1 cut(s)	2313
Bse8I	GATNNNNATC	2 cut(s)	1478, 1683
BseAI	TCCGGA	1 cut(s)	384
BseDI	CCNNGG	8 cut(s)	363, 1079, 1432, 1469, 1623, 2015, 2145, 2410
BseGI	GGATG	5 cut(s)	514, 709, 807, 1594, 1883
BseJI	GATNNNNATC	2 cut(s)	1478, 1683
BseLI	CCNNNNNNNGG	7 cut(s)	325, 435, 446, 704, 928, 1166, 1681
BseMI	GCAATG	1 cut(s)	2313
BseMII	CTCAG	2 cut(s)	312, 1275
BseNI	ACTGG	4 cut(s)	677, 1212, 1552, 2374
BseSI	GKGCMC	1 cut(s)	2418
BseYI	CCCAGC	2 cut(s)	34, 662
BsgI	GTGCAG	1 cut(s)	1794
BshFI	GGCC	7 cut(s)	439, 553, 777, 934, 1927, 2004, 2151
BsiHKAI	GWGCWC	1 cut(s)	349
BsiHKCI	CYCGRG	1 cut(s)	1644
BsiSI	CCGG	7 cut(s)	350, 385, 429, 1114, 1915, 2410, 2432
BsiWI	CGTACG	1 cut(s)	546
BslFI	GGGAC	2 cut(s)	271, 1723
BslI	CCNNNNNNNGG	7 cut(s)	325, 435, 446, 704, 928, 1166, 1681
BsmAI	GTCTC	3 cut(s)	1267, 1375, 2354
BsmFI	GGGAC	2 cut(s)	271, 1723
BsmI	GAATGC	2 cut(s)	246, 774
BsnI	GGCC	7 cut(s)	439, 553, 777, 934, 1927, 2004, 2151
Bso31I	GGTCTC	1 cut(s)	1267
BsoBI	CYCGRG	1 cut(s)	1644
Bsp1286I	GDGCHC	4 cut(s)	349, 634, 1868, 2418
Bsp13I	TCCGGA	1 cut(s)	384
Bsp1407I	TGTACA	2 cut(s)	58, 2081
Bsp19I	CCATGG	1 cut(s)	2145
BspACI	CCGC	3 cut(s)	879, 1682, 1814
BspANI	GGCC	7 cut(s)	439, 553, 777, 934, 1927, 2004, 2151
BspCNI	CTCAG	2 cut(s)	313, 1274
BspEI	TCCGGA	1 cut(s)	384
BspMAI	CTGCAG	1 cut(s)	733
BspPI	GGATC	9 cut(s)	55, 536, 1442, 1512, 1692, 2006, 2024, 2422, 2435
BspQI	GCTCTTC	1 cut(s)	596
BspTNI	GGTCTC	1 cut(s)	1267
BsrDI	GCAATG	1 cut(s)	2313
BsrGI	TGTACA	2 cut(s)	58, 2081
BsrI	ACTGG	4 cut(s)	677, 1212, 1552, 2374
BssECI	CCNNGG	8 cut(s)	363, 1079, 1432, 1469, 1623, 2015, 2145, 2410
BssNI	GRCGYC	1 cut(s)	1598
BssT1I	CCWWGG	3 cut(s)	1079, 2015, 2145
Bst4CI	ACNGT	8 cut(s)	411, 536, 941, 2185, 2212, 2224, 2381, 2457
Bst6I	CTCTTC	1 cut(s)	596
BstACI	GRCGYC	1 cut(s)	1598
BstAPI	GCANNNNNTGC	1 cut(s)	737
BstAUI	TGTACA	2 cut(s)	58, 2081
BstBAI	YACGTR	1 cut(s)	2068
BstC8I	GCNNGC	4 cut(s)	327, 345, 529, 1348
BstDEI	CTNAG	3 cut(s)	321, 917, 1261
BstDSI	CCRYGG	2 cut(s)	363, 2145
BstF5I	GGATG	5 cut(s)	514, 709, 807, 1594, 1883
BstMAI	GTCTC	3 cut(s)	1267, 1375, 2354
BstNSI	RCATGY	1 cut(s)	579
BstSFI	CTRYAG	5 cut(s)	274, 729, 1572, 1908, 2377
BstSLI	GKGCMC	1 cut(s)	2418
BstX2I	RGATCY	5 cut(s)	47, 1504, 1684, 2029, 2427
BstXI	CCANNNNNNTGG	2 cut(s)	151, 785
BstYI	RGATCY	5 cut(s)	47, 1504, 1684, 2029, 2427
BsuRI	GGCC	7 cut(s)	439, 553, 777, 934, 1927, 2004, 2151
BtgI	CCRYGG	2 cut(s)	363, 2145
BtrI	CACGTC	2 cut(s)	195, 2050
BtsCI	GGATG	5 cut(s)	514, 709, 807, 1594, 1883
BtsI	GCAGTG	2 cut(s)	144, 726
BtsIMutI	CAGTG	6 cut(s)	144, 726, 933, 1205, 1482, 2229
Cac8I	GCNNGC	4 cut(s)	327, 345, 529, 1348
Cfr13I	GGNCC	8 cut(s)	258, 382, 551, 932, 1046, 1649, 1737, 2149
CseI	GACGC	1 cut(s)	1606
CsiI	ACCWGGT	2 cut(s)	2089, 2320
Csp6I	GTAC	9 cut(s)	59, 75, 537, 547, 565, 573, 1201, 2082, 2208
CspCI	CAANNNNNGTGG	4 cut(s)	50, 85, 778, 813
CviAII	CATG	9 cut(s)	224, 576, 716, 773, 779, 1786, 2006, 2105, 2146
CviQI	GTAC	9 cut(s)	59, 75, 537, 547, 565, 573, 1201, 2082, 2208
DdeI	CTNAG	3 cut(s)	321, 917, 1261
DraI	TTTAAA	1 cut(s)	1287
DrdI	GACNNNNNNGTC	1 cut(s)	720
DseDI	GACNNNNNNGTC	1 cut(s)	720
EaeI	YGGCCR	3 cut(s)	437, 775, 2002
Eam1104I	CTCTTC	1 cut(s)	596
EarI	CTCTTC	1 cut(s)	596
Eco130I	CCWWGG	3 cut(s)	1079, 2015, 2145
Eco147I	AGGCCT	1 cut(s)	1927
Eco24I	GRGCYC	2 cut(s)	634, 1868
Eco31I	GGTCTC	1 cut(s)	1267
Eco47I	GGWCC	5 cut(s)	258, 382, 1046, 1649, 1737
Eco57I	CTGAAG	2 cut(s)	99, 1224
Eco88I	CYCGRG	1 cut(s)	1644
EcoO109I	RGGNCCY	3 cut(s)	258, 1046, 1649
EcoRI	GAATTC	2 cut(s)	756, 1464
EcoT14I	CCWWGG	3 cut(s)	1079, 2015, 2145
EcoT22I	ATGCAT	1 cut(s)	774
EcoT38I	GRGCYC	2 cut(s)	634, 1868
ErhI	CCWWGG	3 cut(s)	1079, 2015, 2145
FaeI	CATG	9 cut(s)	227, 579, 719, 776, 782, 1789, 2009, 2108, 2149
FalI	AAGNNNNNCTT	4 cut(s)	590, 622, 1871, 1903
FaqI	GGGAC	2 cut(s)	271, 1723
FatI	CATG	9 cut(s)	223, 575, 715, 772, 778, 1785, 2005, 2104, 2145
FbaI	TGATCA	1 cut(s)	886
FokI	GGATG	5 cut(s)	501, 716, 814, 1601, 1870
FriOI	GRGCYC	2 cut(s)	634, 1868
FspBI	CTAG	4 cut(s)	180, 1706, 2027, 2331
GsaI	CCCAGC	2 cut(s)	38, 666
GsuI	CTGGAG	5 cut(s)	486, 930, 1916, 2259, 2286
HaeIII	GGCC	7 cut(s)	439, 553, 777, 934, 1927, 2004, 2151
HapII	CCGG	7 cut(s)	350, 385, 429, 1114, 1915, 2410, 2432
HgaI	GACGC	1 cut(s)	1606
Hin1I	GRCGYC	1 cut(s)	1598
Hin1II	CATG	9 cut(s)	227, 579, 719, 776, 782, 1789, 2009, 2108, 2149
HincII	GTYRAC	1 cut(s)	586
HindII	GTYRAC	1 cut(s)	586
HindIII	AAGCTT	1 cut(s)	520
HinfI	GANTC	5 cut(s)	155, 185, 494, 1235, 2347
HpaII	CCGG	7 cut(s)	350, 385, 429, 1114, 1915, 2410, 2432
HphI	GGTGA	3 cut(s)	134, 719, 1167
Hpy166II	GTNNAC	3 cut(s)	586, 1203, 2082
Hpy188I	TCNGA	6 cut(s)	160, 767, 1264, 1375, 1722, 1951
Hpy188III	TCNNGA	9 cut(s)	45, 385, 1363, 1461, 1536, 1794, 2027, 2240, 2331
Hpy8I	GTNNAC	3 cut(s)	586, 1203, 2082
Hpy99I	CGWCG	2 cut(s)	2364, 2457
HpyAV	CCTTC	3 cut(s)	373, 1467, 1917
HpyCH4III	ACNGT	8 cut(s)	411, 536, 941, 2185, 2212, 2224, 2381, 2457
HpyCH4IV	ACGT	6 cut(s)	24, 194, 1199, 2049, 2067, 2362
HpyF3I	CTNAG	3 cut(s)	321, 917, 1261
HpySE526I	ACGT	6 cut(s)	24, 194, 1199, 2049, 2067, 2362
Hsp92I	GRCGYC	1 cut(s)	1598
Hsp92II	CATG	9 cut(s)	227, 579, 719, 776, 782, 1789, 2009, 2108, 2149
Kpn2I	TCCGGA	1 cut(s)	384
Ksp22I	TGATCA	1 cut(s)	886
LguI	GCTCTTC	1 cut(s)	596
LmnI	GCTCC	6 cut(s)	352, 629, 811, 1714, 1863, 2278
LweI	GCATC	5 cut(s)	295, 973, 1364, 1669, 2089
MabI	ACCWGGT	2 cut(s)	2089, 2320
MaeI	CTAG	4 cut(s)	180, 1706, 2027, 2331
MaeII	ACGT	6 cut(s)	24, 194, 1199, 2049, 2067, 2362
MaeIII	GTNAC	6 cut(s)	190, 611, 722, 1173, 2212, 2224
MboII	GAAGA	5 cut(s)	613, 1230, 1307, 1679, 2255
MfeI	CAATTG	3 cut(s)	882, 1398, 2280
MflI	RGATCY	5 cut(s)	47, 1504, 1684, 2029, 2427
MhlI	GDGCHC	4 cut(s)	349, 634, 1868, 2418
MlsI	TGGCCA	3 cut(s)	439, 777, 2004
MluNI	TGGCCA	3 cut(s)	439, 777, 2004
MlyI	GAGTC	1 cut(s)	2356
MmeI	TCCRAC	1 cut(s)	449
Mox20I	TGGCCA	3 cut(s)	439, 777, 2004
Mph1103I	ATGCAT	1 cut(s)	774
MroI	TCCGGA	1 cut(s)	384
MroXI	GAANNNNTTC	1 cut(s)	605
MscI	TGGCCA	3 cut(s)	439, 777, 2004
MseI	TTAA	6 cut(s)	672, 1286, 1491, 1557, 1659, 1965
MslI	CAYNNNNRTG	2 cut(s)	149, 777
Msp20I	TGGCCA	3 cut(s)	439, 777, 2004
MspA1I	CMGCKG	4 cut(s)	662, 743, 1001, 1034
MspI	CCGG	7 cut(s)	350, 385, 429, 1114, 1915, 2410, 2432
MunI	CAATTG	3 cut(s)	882, 1398, 2280
Mva1269I	GAATGC	2 cut(s)	246, 774
NciI	CCSGG	4 cut(s)	351, 1916, 2411, 2432
NcoI	CCATGG	1 cut(s)	2145
NlaIII	CATG	9 cut(s)	227, 579, 719, 776, 782, 1789, 2009, 2108, 2149
NmuCI	GTSAC	4 cut(s)	190, 722, 1173, 2224
NsiI	ATGCAT	1 cut(s)	774
NspI	RCATGY	1 cut(s)	579
OliI	CACNNNNGTG	1 cut(s)	149
PaeR7I	CTCGAG	1 cut(s)	1644
PceI	AGGCCT	1 cut(s)	1927
PciI	ACATGT	1 cut(s)	575
PciSI	GCTCTTC	1 cut(s)	596
PctI	GAATGC	2 cut(s)	246, 774
PdmI	GAANNNNTTC	1 cut(s)	605
PfeI	GAWTC	4 cut(s)	155, 185, 494, 1235
Pfl23II	CGTACG	1 cut(s)	546
PfoI	TCCNGGA	1 cut(s)	2032
PleI	GAGTC	1 cut(s)	2355
PpsI	GAGTC	1 cut(s)	2355
Ppu21I	YACGTR	1 cut(s)	2068
PpuMI	RGGWCCY	3 cut(s)	258, 1046, 1649
PscI	ACATGT	1 cut(s)	575
PsiI	TTATAA	3 cut(s)	110, 293, 1841
Psp5II	RGGWCCY	3 cut(s)	258, 1046, 1649
PspFI	CCCAGC	2 cut(s)	34, 662
PspLI	CGTACG	1 cut(s)	546
PspPI	GGNCC	8 cut(s)	258, 382, 551, 932, 1046, 1649, 1737, 2149
PspPPI	RGGWCCY	3 cut(s)	258, 1046, 1649
PspXI	VCTCGAGB	1 cut(s)	1644
PstI	CTGCAG	1 cut(s)	733
PsuI	RGATCY	5 cut(s)	47, 1504, 1684, 2029, 2427
PvuII	CAGCTG	4 cut(s)	662, 743, 1001, 1034
RsaI	GTAC	9 cut(s)	60, 76, 538, 548, 566, 574, 1202, 2083, 2209
RsaNI	GTAC	9 cut(s)	59, 75, 537, 547, 565, 573, 1201, 2082, 2208
RseI	CAYNNNNRTG	2 cut(s)	149, 777
SapI	GCTCTTC	1 cut(s)	596
SaqAI	TTAA	6 cut(s)	672, 1286, 1491, 1557, 1659, 1965
Sau96I	GGNCC	8 cut(s)	258, 382, 551, 932, 1046, 1649, 1737, 2149
ScaI	AGTACT	2 cut(s)	76, 538
SchI	GAGTC	1 cut(s)	2356
SduI	GDGCHC	4 cut(s)	349, 634, 1868, 2418
SexAI	ACCWGGT	2 cut(s)	2089, 2320
SfaNI	GCATC	5 cut(s)	295, 973, 1364, 1669, 2089
SfcI	CTRYAG	5 cut(s)	274, 729, 1572, 1908, 2377
Sfr274I	CTCGAG	1 cut(s)	1644
SinI	GGWCC	5 cut(s)	258, 382, 1046, 1649, 1737
SlaI	CTCGAG	1 cut(s)	1644
SmiMI	CAYNNNNRTG	2 cut(s)	149, 777
SmlI	CTYRAG	3 cut(s)	212, 1644, 2238
SmoI	CTYRAG	3 cut(s)	212, 1644, 2238
SpeI	ACTAGT	1 cut(s)	179
SseBI	AGGCCT	1 cut(s)	1927
SsiI	CCGC	3 cut(s)	879, 1682, 1814
SspI	AATATT	5 cut(s)	475, 1155, 1312, 1635, 1657
SspMI	CTAG	4 cut(s)	180, 1706, 2027, 2331
StuI	AGGCCT	1 cut(s)	1927
StyI	CCWWGG	3 cut(s)	1079, 2015, 2145
TaaI	ACNGT	8 cut(s)	411, 536, 941, 2185, 2212, 2224, 2381, 2457
TaiI	ACGT	6 cut(s)	27, 197, 1202, 2052, 2070, 2365
TaqI	TCGA	6 cut(s)	171, 205, 1195, 1552, 1645, 2359
TatI	WGTACW	5 cut(s)	58, 74, 536, 564, 2081
TauI	GCSGC	1 cut(s)	881
TfiI	GAWTC	4 cut(s)	155, 185, 494, 1235
Tru1I	TTAA	6 cut(s)	672, 1286, 1491, 1557, 1659, 1965
Tru9I	TTAA	6 cut(s)	672, 1286, 1491, 1557, 1659, 1965
TscAI	CASTG	6 cut(s)	151, 733, 933, 1212, 1489, 2229
TseFI	GTSAC	4 cut(s)	190, 722, 1173, 2224
Tsp45I	GTSAC	4 cut(s)	190, 722, 1173, 2224
TspDTI	ATGAA	4 cut(s)	17, 177, 299, 795
TspGWI	ACGGA	4 cut(s)	352, 534, 630, 2163
TspRI	CASTG	6 cut(s)	151, 733, 933, 1212, 1489, 2229
VpaK11BI	GGWCC	5 cut(s)	258, 382, 1046, 1649, 1737
XapI	RAATTY	5 cut(s)	756, 782, 1442, 1464, 2161
XbaI	TCTAGA	2 cut(s)	2026, 2330
XceI	RCATGY	1 cut(s)	579
XcmI	CCANNNNNNNNNTGG	2 cut(s)	432, 1136
XhoI	CTCGAG	1 cut(s)	1644
XmnI	GAANNNNTTC	1 cut(s)	605
XspI	CTAG	4 cut(s)	180, 1706, 2027, 2331
ZrmI	AGTACT	2 cut(s)	76, 538
Zsp2I	ATGCAT	1 cut(s)	774

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.