Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0002949

Physical Location & Seq

Genomic Coordinates Forward (+)

20449536 .. 20470232

Gene Structure 20697 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG096300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2463 bp

ATGGTAAAACTGAGGGCTATGGAGTTTTGGTATGCCGTTTTGGTATTGTTTTCCCTTTTCGTTATTGGGAAGGCTGATGTTTATATTGTGACTGTCGAAGGAGAGCCTATTGTAAGTTATAGAGGTGATGTTGATGGTTTTGAAGCCACTGCTGTGGAATCTGATGAAAAGATCGACACCACTAGTGAATCAGTCACGTCCTATGCTCGACACCTTGAGAATAAACATGATATGCTTCTTGGAATGCTGTTTGAGCAAGGGACCTACAAGAAACTCTACAGCTATCAGCATCTTATAAATGGCTTTGCAGTTCATATTTCTCCTGAGCAGGCAGAAAGACTAATGCGTGCTCCGGGTGTGAAATCCGTGGAGAGAGATTGGAAGGTCCGGAGGCTTACAACTCACACACCACAGTTTTTAGGGCTTCCAACCGGAGTATGGCCAACAGGAGGTGGCTTTGATAGGGCTGGAGAAAATATTGTGATAGGGTTTGTGGACTCGGGGATTTACCCTCATCATCCAAGCTTTGCAAGCCCCAGTACTGATCCTTATGGGCCTGTTCCCAAGTACAGAGGTACATGTGAAGTTGACCCAGACACCAAGAAGAGCTTCTGTAACGGAAAGATTATTGGAGCCCACCATTTCGCCAAAGCTGCAATAGCAGCTGGGGTGTTTAATCCAGTTATTGATTTCGCCTCTCCTATGGATGGTGATGGACATGGAAGTCACACTGCAGCTATTGCAGCTGGAAACAATGGAATTCCTGTCAGAATGCATGGCCATGAATTTGGCAAAGCAAGTGGGATGGCTCCCCGTGCCAGAATTGCTGTATATAAAGCTCTTTACAGGCTCTTTGGAGGGTTTGTAGCAGATGTAGTAGCCGCAATTGATCAGGCTGTTTATGATGGAGTGGATATACTGAGCCTTTCTGTGGGGCCAAACAGTCCTCCAGCTACCACCAAGACCACTTATTTGAACCCGTTTGATGCCACACTTCTTTCAGCTGTGAAAGCGGGTGTATTTGTAGCACAGGCAGCTGGAAATGGAGGACCTTTTCCGAAAACTTTGGTTTCTTATAGCCCTTGGATAGCTTCTGTTGCTGCTGCCATAGATGACCGGAGATACAAAAACCATTTCACGCTTGGGAATGGAAAAATATTACCTGGACTTGGGTTGTCACCATCTACACATCCAAATCGAACGTACGCACTGGTTGCTGCAAATGATGTGCTTTTGGATTCTTCAGTAGTGAAGTATAGCCCCTCAGACTGCCAGAGACCAGAAGTTTTAAACAAGAACTTGGTTGAGGGGAATATTCTTCTTTGTGGCTATTCGTTCAATTTTGTTGCTGGCTCTGCATCAATCAAGAAAGTCTCGGAAACAGCTAGAAGTCTTGGTGCAATTGGCTTTGTTCTCGCTGTGGAAAATGTTTCCCCAGGAACCAAATTTGATCCTGTTCCTTCTGGAATTCCTGGGATTGTAATCACTGATGTTAAGAAGTCAATGGATCTTATAGATTACTACAACATCTCCACATCAAGAGATTGGACTGGTCGAGTTAAGAGTTTCAAAGCTATAGGTAGCATTGGGGATGGTTTGACGCCTATACTCCATAAATCAGCACCCCAGGTGGCAATATTCTCTGCTCGAGGACCCAATATTAAAGATTTCAGCTTCCAAGATGCGGATCTTCTCAAACCAGATATTCTAGCTCCTGGTTCTCTGATTTGGGCTGCCTGGTCCCCTAATGGAACAGATGAGCCTGATTATGTTGGGGAGGGATTTGCCATGATTTCTGGAACAAGTATGGCTGCACCGCATATAGCAGGAATTGCTGCTCTTATAAAGCAGAAGCACCCTCATTGGAGCCCTGCTGCCATCAAGTCATCCTTGATGACAACATCAACAACTATAGACCGGGCTGGAAGGCCTCTCCAGGCACAACAGACTTCCGAAACACAAGGCATTAAGTTTGTGAGTGCTACTCCTTTTGATTATGGTAGTGGTCATGTTGATCCAAGGGCTGCTCTAGATCCTGGACTAATCTTTGACGTGGGTTTTGAGGATTACTTGGGCTTCTTGTGTACAACACCTGGTATTGATGCCCATGAGATAAAGAAATACACAAACTCCCCCTGCAATTACACCATGGGCCACCCATCAAATTTCAACTCTCCGTCAATCACAGTATCTCATCTTGTAGGAAGTCGTACAGTTACTCGCACAGTGACAAATGTTGCTCAAGAAGAAACCTATGTGATCACAGCCAGAATGGCTCCAGCAATTGCCATTAGTGTCAGCCCTCCGGCAATGACTCTGAAACCTGGTGCTTCTAGAAAGTTCTCTGTGAGTCTCACAGCTCGACGTTTGACTGGAACCTACAGTTTTGGAGAGGTTCTGTTGAAAGGTAGCCGGGGGCACAAGGTTAGGATCCCGGTGGTAGCTATGGGTTACCGACGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8 fn3_5

Protein Analysis

820

Amino Acids

87.88

Weight (kDa)

8.67

Isoelectric Point (pI)

29.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	28 - 130	3.6e-15	Peptidase inhibitor I9
Peptidase_S8	PF00082	157 - 643	2.1e-40	Subtilase family
fn3_5	PF06280	717 - 814	3e-17	Fn3-like domain
fn3_6	PF17766	722 - 814	2.5e-18	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002949)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19170 AT2G19170 AT4G30020 AT4G30020 AT4G30020 AT4G30020
fragaria_vesca	FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370 FvH4_4g07370
malus_domestica	MD13G1242900.v1.1 MD16G1249700.v1.1
prunus_persica	Prupe.1G076600_v2.0.a1 Prupe.1G076600_v2.0.a1 Prupe.1G076600_v2.0.a1 Prupe.1G076600_v2.0.a1
pyrus_communis	pycom13g21460 pycom16g20920 pycom16g20930
rosa_chinensis	RchiOBHm_Chr4g0399741 RchiOBHm_Chr4g0399751
rosa_laevigata	RLG00000009242
rosa_multiflora	Rmu_sc0005299.1_g000011 Rmu_sc0028740.1_g000001
rosa_roxburghii	Rroxscaffold_5G00344690
rosa_rugosa	Rorug04G0018500 Rorug04G0018600 Rorug04G0018700
rosa_samantha	Rh4AG096300 Rh4BG093700 Rh4CG105200 Rh4DG090100
rosa_wichuraiana	Rw4G007960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	296, 1844
AasI	GACNNNNNNGTC	1 cut(s)	723
AccIII	TCCGGA	1 cut(s)	387
AciI	CCGC	4 cut(s)	882, 1013, 1685, 1817
AclWI	GGATC	8 cut(s)	539, 1445, 1515, 1695, 2009, 2027, 2425, 2438
AcoI	YGGCCR	2 cut(s)	440, 778
AcsI	RAATTY	5 cut(s)	759, 785, 1445, 1467, 2164
AcuI	CTGAAG	1 cut(s)	1227
AcyI	GRCGYC	1 cut(s)	1601
AfaI	GTAC	6 cut(s)	541, 569, 577, 1205, 2086, 2212
AfiI	CCNNNNNNNGG	6 cut(s)	438, 449, 707, 931, 1169, 1684
AflIII	ACRYGT	1 cut(s)	578
AgsI	TTSAA	6 cut(s)	143, 976, 1339, 1570, 2170, 2404
AhlI	ACTAGT	1 cut(s)	182
AjiI	CACGTC	2 cut(s)	198, 2053
AjuI	GAANNNNNNNTTGG	2 cut(s)	593, 625
AleI	CACNNNNGTG	1 cut(s)	152
Alw21I	GWGCWC	1 cut(s)	352
Alw26I	GTCTC	3 cut(s)	1270, 1378, 2357
AlwI	GGATC	8 cut(s)	539, 1445, 1515, 1695, 2009, 2027, 2425, 2438
Ama87I	CYCGRG	2 cut(s)	499, 1647
Aor13HI	TCCGGA	1 cut(s)	387
AoxI	GGCC	6 cut(s)	440, 554, 778, 935, 1928, 2152
ApoI	RAATTY	5 cut(s)	759, 785, 1445, 1467, 2164
ArsI	GACNNNNNNTTYG	4 cut(s)	955, 987, 2042, 2074
Asp700I	GAANNNNTTC	1 cut(s)	608
AspS9I	GGNCC	8 cut(s)	261, 385, 554, 935, 1049, 1652, 1740, 2152
AsuC2I	CCSGG	4 cut(s)	354, 1919, 2414, 2435
AsuHPI	GGTGA	3 cut(s)	137, 722, 1170
AvaI	CYCGRG	2 cut(s)	499, 1647
AvaII	GGWCC	5 cut(s)	261, 385, 1049, 1652, 1740
BaeGI	GKGCMC	1 cut(s)	2421
BalI	TGGCCA	2 cut(s)	442, 780
BamHI	GGATCC	1 cut(s)	2430
BanII	GRGCYC	2 cut(s)	637, 1871
Bbv12I	GWGCWC	1 cut(s)	352
BccI	CCATC	9 cut(s)	128, 701, 707, 799, 899, 1189, 1586, 1886, 2167
BceAI	ACGGC	1 cut(s)	20
BclI	TGATCA	2 cut(s)	889, 2259
BcnI	CCSGG	4 cut(s)	354, 1919, 2414, 2435
BcoDI	GTCTC	3 cut(s)	1270, 1378, 2357
BcuI	ACTAGT	1 cut(s)	182
BfaI	CTAG	5 cut(s)	183, 1386, 1709, 2030, 2334
BfmI	CTRYAG	5 cut(s)	277, 732, 1575, 1911, 2380
BglI	GCCNNNNNGGC	1 cut(s)	2273
BmcAI	AGTACT	1 cut(s)	541
Bme18I	GGWCC	5 cut(s)	261, 385, 1049, 1652, 1740
BmeT110I	CYCGRG	2 cut(s)	499, 1647
BmgBI	CACGTC	2 cut(s)	198, 2053
BmgT120I	GGNCC	8 cut(s)	261, 385, 554, 935, 1049, 1652, 1740, 2152
BmrI	ACTGGG	1 cut(s)	531
BmsI	GCATC	5 cut(s)	298, 976, 1367, 1672, 2092
BmuI	ACTGGG	1 cut(s)	531
BplI	GAGNNNNNCTC	2 cut(s)	1969, 2001
BpmI	CTGGAG	4 cut(s)	489, 933, 1919, 2262
Bpu10I	CCTNAGC	1 cut(s)	324
BpuEI	CTTGAG	2 cut(s)	236, 2226
BpuMI	CCSGG	4 cut(s)	354, 1919, 2414, 2435
BsaBI	GATNNNNATC	2 cut(s)	1481, 1686
BsaHI	GRCGYC	1 cut(s)	1601
BsaI	GGTCTC	1 cut(s)	1270
BsaJI	CCNNGG	8 cut(s)	366, 1082, 1435, 1472, 1626, 2018, 2148, 2413
BsaWI	WCCGGW	3 cut(s)	387, 431, 1116
BsaXI	ACNNNNNCTCC	8 cut(s)	14, 44, 849, 879, 1514, 1544, 2114, 2144
Bsc4I	CCNNNNNNNGG	6 cut(s)	438, 449, 707, 931, 1169, 1684
Bse1I	ACTGG	5 cut(s)	537, 680, 1215, 1555, 2377
Bse3DI	GCAATG	1 cut(s)	2316
Bse8I	GATNNNNATC	2 cut(s)	1481, 1686
BseAI	TCCGGA	1 cut(s)	387
BseDI	CCNNGG	8 cut(s)	366, 1082, 1435, 1472, 1626, 2018, 2148, 2413
BseGI	GGATG	6 cut(s)	517, 712, 810, 1189, 1597, 1886
BseJI	GATNNNNATC	2 cut(s)	1481, 1686
BseLI	CCNNNNNNNGG	6 cut(s)	438, 449, 707, 931, 1169, 1684
BseMI	GCAATG	1 cut(s)	2316
BseMII	CTCAG	3 cut(s)	315, 911, 1278
BseNI	ACTGG	5 cut(s)	537, 680, 1215, 1555, 2377
BseSI	GKGCMC	1 cut(s)	2421
BseYI	CCCAGC	1 cut(s)	665
BsgI	GTGCAG	1 cut(s)	1797
BshFI	GGCC	6 cut(s)	442, 556, 780, 937, 1930, 2154
BsiHKAI	GWGCWC	1 cut(s)	352
BsiHKCI	CYCGRG	2 cut(s)	499, 1647
BsiSI	CCGG	8 cut(s)	353, 388, 432, 1117, 1918, 2306, 2413, 2435
BsiWI	CGTACG	1 cut(s)	1203
BslFI	GGGAC	2 cut(s)	274, 1726
BslI	CCNNNNNNNGG	6 cut(s)	438, 449, 707, 931, 1169, 1684
BsmAI	GTCTC	3 cut(s)	1270, 1378, 2357
BsmFI	GGGAC	2 cut(s)	274, 1726
BsmI	GAATGC	2 cut(s)	249, 777
BsnI	GGCC	6 cut(s)	442, 556, 780, 937, 1930, 2154
Bso31I	GGTCTC	1 cut(s)	1270
BsoBI	CYCGRG	2 cut(s)	499, 1647
Bsp1286I	GDGCHC	4 cut(s)	352, 637, 1871, 2421
Bsp13I	TCCGGA	1 cut(s)	387
Bsp1407I	TGTACA	1 cut(s)	2084
Bsp19I	CCATGG	1 cut(s)	2148
BspACI	CCGC	4 cut(s)	882, 1013, 1685, 1817
BspANI	GGCC	6 cut(s)	442, 556, 780, 937, 1930, 2154
BspCNI	CTCAG	3 cut(s)	316, 912, 1277
BspEI	TCCGGA	1 cut(s)	387
BspMAI	CTGCAG	1 cut(s)	736
BspPI	GGATC	8 cut(s)	539, 1445, 1515, 1695, 2009, 2027, 2425, 2438
BspQI	GCTCTTC	1 cut(s)	599
BspTNI	GGTCTC	1 cut(s)	1270
BsrDI	GCAATG	1 cut(s)	2316
BsrGI	TGTACA	1 cut(s)	2084
BsrI	ACTGG	5 cut(s)	537, 680, 1215, 1555, 2377
BssECI	CCNNGG	8 cut(s)	366, 1082, 1435, 1472, 1626, 2018, 2148, 2413
BssNI	GRCGYC	1 cut(s)	1601
BssT1I	CCWWGG	3 cut(s)	1082, 2018, 2148
Bst4CI	ACNGT	8 cut(s)	94, 414, 944, 2188, 2215, 2227, 2384, 2460
Bst6I	CTCTTC	1 cut(s)	599
BstACI	GRCGYC	1 cut(s)	1601
BstAPI	GCANNNNNTGC	3 cut(s)	740, 1214, 1832
BstAUI	TGTACA	1 cut(s)	2084
BstC8I	GCNNGC	4 cut(s)	330, 348, 532, 1351
BstDEI	CTNAG	4 cut(s)	11, 324, 920, 1264
BstDSI	CCRYGG	2 cut(s)	366, 2148
BstEII	GGTNACC	1 cut(s)	2450
BstF5I	GGATG	6 cut(s)	517, 712, 810, 1189, 1597, 1886
BstMAI	GTCTC	3 cut(s)	1270, 1378, 2357
BstNSI	RCATGY	1 cut(s)	582
BstPI	GGTNACC	1 cut(s)	2450
BstSFI	CTRYAG	5 cut(s)	277, 732, 1575, 1911, 2380
BstSLI	GKGCMC	1 cut(s)	2421
BstX2I	RGATCY	4 cut(s)	1507, 1687, 2032, 2430
BstXI	CCANNNNNNTGG	2 cut(s)	154, 788
BstYI	RGATCY	4 cut(s)	1507, 1687, 2032, 2430
BsuRI	GGCC	6 cut(s)	442, 556, 780, 937, 1930, 2154
BtgI	CCRYGG	2 cut(s)	366, 2148
BtrI	CACGTC	2 cut(s)	198, 2053
BtsCI	GGATG	6 cut(s)	517, 712, 810, 1189, 1597, 1886
BtsI	GCAGTG	2 cut(s)	147, 729
BtsIMutI	CAGTG	5 cut(s)	147, 729, 1208, 1485, 2232
Cac8I	GCNNGC	4 cut(s)	330, 348, 532, 1351
Cfr13I	GGNCC	8 cut(s)	261, 385, 554, 935, 1049, 1652, 1740, 2152
CseI	GACGC	1 cut(s)	1609
CsiI	ACCWGGT	2 cut(s)	2092, 2323
Csp6I	GTAC	6 cut(s)	540, 568, 576, 1204, 2085, 2211
CspCI	CAANNNNNGTGG	2 cut(s)	781, 816
CviAII	CATG	9 cut(s)	227, 579, 719, 776, 782, 1789, 2009, 2108, 2149
CviQI	GTAC	6 cut(s)	540, 568, 576, 1204, 2085, 2211
DdeI	CTNAG	4 cut(s)	11, 324, 920, 1264
DraI	TTTAAA	1 cut(s)	1290
DrdI	GACNNNNNNGTC	1 cut(s)	723
DseDI	GACNNNNNNGTC	1 cut(s)	723
EaeI	YGGCCR	2 cut(s)	440, 778
Eam1104I	CTCTTC	1 cut(s)	599
EarI	CTCTTC	1 cut(s)	599
Eco130I	CCWWGG	3 cut(s)	1082, 2018, 2148
Eco147I	AGGCCT	1 cut(s)	1930
Eco24I	GRGCYC	2 cut(s)	637, 1871
Eco31I	GGTCTC	1 cut(s)	1270
Eco47I	GGWCC	5 cut(s)	261, 385, 1049, 1652, 1740
Eco57I	CTGAAG	1 cut(s)	1227
Eco88I	CYCGRG	2 cut(s)	499, 1647
Eco91I	GGTNACC	1 cut(s)	2450
EcoO109I	RGGNCCY	3 cut(s)	261, 1049, 1652
EcoO65I	GGTNACC	1 cut(s)	2450
EcoRI	GAATTC	2 cut(s)	759, 1467
EcoT14I	CCWWGG	3 cut(s)	1082, 2018, 2148
EcoT22I	ATGCAT	1 cut(s)	777
EcoT38I	GRGCYC	2 cut(s)	637, 1871
ErhI	CCWWGG	3 cut(s)	1082, 2018, 2148
FaeI	CATG	9 cut(s)	230, 582, 722, 779, 785, 1792, 2012, 2111, 2152
FalI	AAGNNNNNCTT	4 cut(s)	593, 625, 1874, 1906
FaqI	GGGAC	2 cut(s)	274, 1726
FatI	CATG	9 cut(s)	226, 578, 718, 775, 781, 1788, 2008, 2107, 2148
FauI	CCCGC	1 cut(s)	1006
FbaI	TGATCA	2 cut(s)	889, 2259
FokI	GGATG	6 cut(s)	504, 719, 817, 1176, 1604, 1873
FriOI	GRGCYC	2 cut(s)	637, 1871
FspBI	CTAG	5 cut(s)	183, 1386, 1709, 2030, 2334
GsaI	CCCAGC	1 cut(s)	669
GsuI	CTGGAG	4 cut(s)	489, 933, 1919, 2262
HaeIII	GGCC	6 cut(s)	442, 556, 780, 937, 1930, 2154
HapII	CCGG	8 cut(s)	353, 388, 432, 1117, 1918, 2306, 2413, 2435
HgaI	GACGC	1 cut(s)	1609
Hin1I	GRCGYC	1 cut(s)	1601
Hin1II	CATG	9 cut(s)	230, 582, 722, 779, 785, 1792, 2012, 2111, 2152
HincII	GTYRAC	1 cut(s)	589
HindII	GTYRAC	1 cut(s)	589
HindIII	AAGCTT	1 cut(s)	523
HinfI	GANTC	6 cut(s)	158, 188, 497, 1238, 2314, 2350
HpaII	CCGG	8 cut(s)	353, 388, 432, 1117, 1918, 2306, 2413, 2435
HphI	GGTGA	3 cut(s)	137, 722, 1170
Hpy166II	GTNNAC	3 cut(s)	496, 589, 2085
Hpy188I	TCNGA	8 cut(s)	163, 770, 1059, 1267, 1378, 1725, 1954, 2319
Hpy188III	TCNNGA	9 cut(s)	323, 388, 1366, 1464, 1539, 1797, 2030, 2243, 2334
Hpy8I	GTNNAC	3 cut(s)	496, 589, 2085
Hpy99I	CGWCG	2 cut(s)	2367, 2460
HpyAV	CCTTC	5 cut(s)	64, 92, 376, 1470, 1920
HpyCH4III	ACNGT	8 cut(s)	94, 414, 944, 2188, 2215, 2227, 2384, 2460
HpyCH4IV	ACGT	4 cut(s)	197, 1202, 2052, 2365
HpyF3I	CTNAG	4 cut(s)	11, 324, 920, 1264
HpySE526I	ACGT	4 cut(s)	197, 1202, 2052, 2365
Hsp92I	GRCGYC	1 cut(s)	1601
Hsp92II	CATG	9 cut(s)	230, 582, 722, 779, 785, 1792, 2012, 2111, 2152
Kpn2I	TCCGGA	1 cut(s)	387
Ksp22I	TGATCA	2 cut(s)	889, 2259
LguI	GCTCTTC	1 cut(s)	599
LmnI	GCTCC	6 cut(s)	355, 632, 814, 1717, 1866, 2281
LweI	GCATC	5 cut(s)	298, 976, 1367, 1672, 2092
MabI	ACCWGGT	2 cut(s)	2092, 2323
MaeI	CTAG	5 cut(s)	183, 1386, 1709, 2030, 2334
MaeII	ACGT	4 cut(s)	197, 1202, 2052, 2365
MaeIII	GTNAC	8 cut(s)	88, 193, 614, 725, 1176, 2215, 2227, 2450
MboII	GAAGA	5 cut(s)	616, 1233, 1310, 1682, 2258
MfeI	CAATTG	3 cut(s)	885, 1401, 2283
MflI	RGATCY	4 cut(s)	1507, 1687, 2032, 2430
MhlI	GDGCHC	4 cut(s)	352, 637, 1871, 2421
MlsI	TGGCCA	2 cut(s)	442, 780
MluNI	TGGCCA	2 cut(s)	442, 780
MlyI	GAGTC	3 cut(s)	491, 2308, 2359
MmeI	TCCRAC	1 cut(s)	452
Mox20I	TGGCCA	2 cut(s)	442, 780
Mph1103I	ATGCAT	1 cut(s)	777
MroI	TCCGGA	1 cut(s)	387
MroXI	GAANNNNTTC	1 cut(s)	608
MscI	TGGCCA	2 cut(s)	442, 780
MseI	TTAA	6 cut(s)	675, 1289, 1494, 1560, 1662, 1968
MslI	CAYNNNNRTG	2 cut(s)	152, 780
Msp20I	TGGCCA	2 cut(s)	442, 780
MspA1I	CMGCKG	4 cut(s)	665, 746, 1004, 1037
MspI	CCGG	8 cut(s)	353, 388, 432, 1117, 1918, 2306, 2413, 2435
MunI	CAATTG	3 cut(s)	885, 1401, 2283
Mva1269I	GAATGC	2 cut(s)	249, 777
NciI	CCSGG	4 cut(s)	354, 1919, 2414, 2435
NcoI	CCATGG	1 cut(s)	2148
NlaIII	CATG	9 cut(s)	230, 582, 722, 779, 785, 1792, 2012, 2111, 2152
NmuCI	GTSAC	5 cut(s)	88, 193, 725, 1176, 2227
NsiI	ATGCAT	1 cut(s)	777
NspI	RCATGY	1 cut(s)	582
OliI	CACNNNNGTG	1 cut(s)	152
PaeR7I	CTCGAG	1 cut(s)	1647
PceI	AGGCCT	1 cut(s)	1930
PciI	ACATGT	1 cut(s)	578
PciSI	GCTCTTC	1 cut(s)	599
PctI	GAATGC	2 cut(s)	249, 777
PdmI	GAANNNNTTC	1 cut(s)	608
PfeI	GAWTC	3 cut(s)	158, 188, 1238
Pfl23II	CGTACG	1 cut(s)	1203
PfoI	TCCNGGA	1 cut(s)	2035
PleI	GAGTC	3 cut(s)	491, 2308, 2358
PpsI	GAGTC	3 cut(s)	491, 2308, 2358
PpuMI	RGGWCCY	3 cut(s)	261, 1049, 1652
PscI	ACATGT	1 cut(s)	578
PsiI	TTATAA	2 cut(s)	296, 1844
Psp5II	RGGWCCY	3 cut(s)	261, 1049, 1652
PspEI	GGTNACC	1 cut(s)	2450
PspFI	CCCAGC	1 cut(s)	665
PspLI	CGTACG	1 cut(s)	1203
PspPI	GGNCC	8 cut(s)	261, 385, 554, 935, 1049, 1652, 1740, 2152
PspPPI	RGGWCCY	3 cut(s)	261, 1049, 1652
PspXI	VCTCGAGB	1 cut(s)	1647
PstI	CTGCAG	1 cut(s)	736
PsuI	RGATCY	4 cut(s)	1507, 1687, 2032, 2430
PvuII	CAGCTG	4 cut(s)	665, 746, 1004, 1037
RsaI	GTAC	6 cut(s)	541, 569, 577, 1205, 2086, 2212
RsaNI	GTAC	6 cut(s)	540, 568, 576, 1204, 2085, 2211
RseI	CAYNNNNRTG	2 cut(s)	152, 780
SapI	GCTCTTC	1 cut(s)	599
SaqAI	TTAA	6 cut(s)	675, 1289, 1494, 1560, 1662, 1968
Sau96I	GGNCC	8 cut(s)	261, 385, 554, 935, 1049, 1652, 1740, 2152
ScaI	AGTACT	1 cut(s)	541
SchI	GAGTC	3 cut(s)	491, 2308, 2359
SduI	GDGCHC	4 cut(s)	352, 637, 1871, 2421
SexAI	ACCWGGT	2 cut(s)	2092, 2323
SfaNI	GCATC	5 cut(s)	298, 976, 1367, 1672, 2092
SfcI	CTRYAG	5 cut(s)	277, 732, 1575, 1911, 2380
Sfr274I	CTCGAG	1 cut(s)	1647
SinI	GGWCC	5 cut(s)	261, 385, 1049, 1652, 1740
SlaI	CTCGAG	1 cut(s)	1647
SmiMI	CAYNNNNRTG	2 cut(s)	152, 780
SmlI	CTYRAG	3 cut(s)	215, 1647, 2241
SmoI	CTYRAG	3 cut(s)	215, 1647, 2241
SpeI	ACTAGT	1 cut(s)	182
SseBI	AGGCCT	1 cut(s)	1930
SsiI	CCGC	4 cut(s)	882, 1013, 1685, 1817
SspI	AATATT	5 cut(s)	478, 1158, 1315, 1638, 1660
SspMI	CTAG	5 cut(s)	183, 1386, 1709, 2030, 2334
StuI	AGGCCT	1 cut(s)	1930
StyI	CCWWGG	3 cut(s)	1082, 2018, 2148
TaaI	ACNGT	8 cut(s)	94, 414, 944, 2188, 2215, 2227, 2384, 2460
TaiI	ACGT	4 cut(s)	200, 1205, 2055, 2368
TaqI	TCGA	7 cut(s)	96, 174, 208, 1198, 1555, 1648, 2362
TatI	WGTACW	3 cut(s)	539, 567, 2084
TauI	GCSGC	1 cut(s)	884
TfiI	GAWTC	3 cut(s)	158, 188, 1238
Tru1I	TTAA	6 cut(s)	675, 1289, 1494, 1560, 1662, 1968
Tru9I	TTAA	6 cut(s)	675, 1289, 1494, 1560, 1662, 1968
TscAI	CASTG	5 cut(s)	154, 736, 1215, 1492, 2232
TseFI	GTSAC	5 cut(s)	88, 193, 725, 1176, 2227
Tsp45I	GTSAC	5 cut(s)	88, 193, 725, 1176, 2227
TspDTI	ATGAA	3 cut(s)	180, 302, 798
TspGWI	ACGGA	3 cut(s)	355, 633, 2166
TspRI	CASTG	5 cut(s)	154, 736, 1215, 1492, 2232
VpaK11BI	GGWCC	5 cut(s)	261, 385, 1049, 1652, 1740
XapI	RAATTY	5 cut(s)	759, 785, 1445, 1467, 2164
XbaI	TCTAGA	2 cut(s)	2029, 2333
XceI	RCATGY	1 cut(s)	582
XcmI	CCANNNNNNNNNTGG	2 cut(s)	435, 1139
XhoI	CTCGAG	1 cut(s)	1647
XmnI	GAANNNNTTC	1 cut(s)	608
XspI	CTAG	5 cut(s)	183, 1386, 1709, 2030, 2334
ZrmI	AGTACT	1 cut(s)	541
Zsp2I	ATGCAT	1 cut(s)	777

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.