MD13G1255100.v1.1

Syntaxin 6, N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009458
Physical Location & Seq
Reverse (-)
27701891 .. 27706975
5085 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1255100.v1.1.491

Sequence Viewer

Length: 1002 bp
ATGATGGTCGCAAGCAGTTTTGATCTTTGGAAAAAGGACGTCTTCTTTCCTGCAGCTGAGGAGGTCCAAGAATCAGCTGACATAATGGAATCTACGTACAGGGTATGGGTTCGACAGAGGAGTGAAAGGCTAGCACCAAAACATTTGGATGAACTCTGTAGGGAGCTGCAAACTGCTTTAGGTACTGCCAAATGGCAGTTGGAAGAGTTTGATAAGGCTGTCAGGTTGAGCTATGGACAACGTGGTGATGATCATACAGCAGCTAGACATGGACAATTTATTACTGCTATTGAAAACCAAATTTCCCAAGTTGAAACAGCACTAAGGGAATCGTTTAGTGTGGAAGGAAAGCAACCCCTTCGATGGGTACAGCTGGATGAAGAAGAACATGATGATTTTGCTGCATTTCTCTCTGGGACATCCCAAAGTATGCGAACTGCTAAAAATGAACGTCTAGAACTCGGACCTTGTGGGAGCAGCTCTGGGGAAAGTGACAATAGAAGAAAAGAGATGAACCTTAAATCTAGTGCTGCATGCAATGGAGACATCTCTGATGAAATAAAGGGTGTTAAAGATGTTATTACTATTTCCAAGGATCCAAATTTCGTCATGGAATCAAAAGGAAAAGAAATTGCAGGAATGAGGGATGATATATTTTATGATACAGAAAAAACAACTAATACAAGGAAGGCATGCAGTTCACCAAATTACGGTGAGCTGAGGATCGTAATAGCGGATGAAGTTGAACAGAGAAGAAATCTAATTCCGGGGTATGAGGACACACCCAAAGAAAAAGGATCTAAACTTGTCTTCTGGAGGCAAAGGTGTGGGCAATTGCATCAGATGTTTGGTCGGGTTGGTGCATCTCAGAAGCAATTGCAAAGTCCACGGAAGCAATGGCAAACACCACTACGCTTGCACAATCGTTGTTCAGTTCATGCTACCCTTGGTTTGATGCTCACTGTTTTTTTGCTTGTACCGTTTGTATTTTATTCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syntaxin-6_N

Protein Analysis

334

Amino Acids

37.95

Weight (kDa)

6.22

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
STX6_10_61_N PF09177 13 - 105 9.7e-19 Syntaxin 6/10/61, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 42
AciI CCGC 1 cut(s) 734
AclWI GGATC 4 cut(s) 590, 603, 731, 805
AcsI RAATTY 2 cut(s) 300, 601
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 4 cut(s) 98, 184, 369, 978
AfiI CCNNNNNNNGG 3 cut(s) 363, 364, 710
AgsI TTSAA 4 cut(s) 293, 314, 746, 996
AluBI AGCT 8 cut(s) 56, 77, 166, 231, 263, 373, 480, 718
AluI AGCT 8 cut(s) 56, 77, 166, 231, 263, 373, 480, 718
Alw26I GTCTC 1 cut(s) 537
AlwI GGATC 4 cut(s) 590, 603, 731, 805
ApeKI GCWGC 6 cut(s) 53, 166, 260, 401, 477, 530
ApoI RAATTY 2 cut(s) 300, 601
AspS9I GGNCC 2 cut(s) 64, 464
AsuC2I CCSGG 1 cut(s) 768
AsuHPI GGTGA 3 cut(s) 257, 693, 725
AsuNHI GCTAGC 1 cut(s) 130
AvaII GGWCC 2 cut(s) 64, 464
BamHI GGATCC 1 cut(s) 595
BbsI GAAGAC 2 cut(s) 34, 802
BbvCI CCTCAGC 2 cut(s) 57, 719
BbvI GCAGC 6 cut(s) 65, 153, 272, 388, 489, 517
BccI CCATC 1 cut(s) 357
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BclI TGATCA 1 cut(s) 250
BcnI CCSGG 1 cut(s) 768
BcoDI GTCTC 1 cut(s) 537
BfaI CTAG 4 cut(s) 131, 264, 455, 525
BfmI CTRYAG 2 cut(s) 51, 157
BisI GCNGC 6 cut(s) 54, 167, 261, 402, 478, 531
BlsI GCNGC 6 cut(s) 55, 168, 262, 403, 479, 532
Bme1390I CCNGG 1 cut(s) 768
Bme18I GGWCC 2 cut(s) 64, 464
BmgT120I GGNCC 2 cut(s) 64, 464
BmiI GGNNCC 1 cut(s) 597
BmrFI CCNGG 1 cut(s) 768
BmsI GCATC 3 cut(s) 847, 872, 945
BmtI GCTAGC 1 cut(s) 134
BpiI GAAGAC 2 cut(s) 34, 802
BpmI CTGGAG 1 cut(s) 835
Bpu10I CCTNAGC 2 cut(s) 57, 719
BpuMI CCSGG 1 cut(s) 768
BsaAI YACGTR 1 cut(s) 96
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 4 cut(s) 591, 767, 887, 946
Bsc4I CCNNNNNNNGG 3 cut(s) 363, 364, 710
Bse3DI GCAATG 2 cut(s) 544, 902
BseDI CCNNGG 4 cut(s) 591, 767, 887, 946
BseGI GGATG 5 cut(s) 154, 382, 419, 652, 742
BseLI CCNNNNNNNGG 3 cut(s) 363, 364, 710
BseMI GCAATG 2 cut(s) 544, 902
BseMII CTCAG 3 cut(s) 48, 710, 881
BseRI GAGGAG 2 cut(s) 74, 133
BseXI GCAGC 6 cut(s) 65, 153, 272, 388, 489, 517
BsiSI CCGG 1 cut(s) 767
BslFI GGGAC 1 cut(s) 430
BslI CCNNNNNNNGG 3 cut(s) 363, 364, 710
BsmAI GTCTC 1 cut(s) 537
BsmFI GGGAC 1 cut(s) 430
Bsp143I GATC 5 cut(s) 22, 250, 595, 723, 797
BspACI CCGC 1 cut(s) 734
BspCNI CTCAG 3 cut(s) 49, 711, 880
BspLI GGNNCC 1 cut(s) 597
BspMAI CTGCAG 1 cut(s) 55
BspOI GCTAGC 1 cut(s) 134
BspPI GGATC 4 cut(s) 590, 603, 731, 805
BsrDI GCAATG 2 cut(s) 544, 902
BssECI CCNNGG 4 cut(s) 591, 767, 887, 946
BssMI GATC 5 cut(s) 22, 250, 595, 723, 797
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 2 cut(s) 591, 946
Bst4CI ACNGT 3 cut(s) 713, 964, 981
Bst6I CTCTTC 1 cut(s) 198
BstACI GRCGYC 1 cut(s) 39
BstBAI YACGTR 1 cut(s) 96
BstC8I GCNNGC 5 cut(s) 13, 132, 535, 694, 917
BstDEI CTNAG 4 cut(s) 57, 323, 719, 867
BstDSI CCRYGG 1 cut(s) 887
BstF5I GGATG 5 cut(s) 154, 382, 419, 652, 742
BstKTI GATC 5 cut(s) 25, 253, 598, 726, 800
BstMAI GTCTC 1 cut(s) 537
BstMBI GATC 5 cut(s) 22, 250, 595, 723, 797
BstNSI RCATGY 2 cut(s) 537, 696
BstSCI CCNGG 1 cut(s) 766
BstSFI CTRYAG 2 cut(s) 51, 157
BstSNI TACGTA 1 cut(s) 96
BstV1I GCAGC 6 cut(s) 65, 153, 272, 388, 489, 517
BstV2I GAAGAC 2 cut(s) 34, 802
BstX2I RGATCY 2 cut(s) 595, 797
BstYI RGATCY 2 cut(s) 595, 797
BtgI CCRYGG 1 cut(s) 887
BtsCI GGATG 5 cut(s) 154, 382, 419, 652, 742
BtsIMutI CAGTG 1 cut(s) 960
Cac8I GCNNGC 5 cut(s) 13, 132, 535, 694, 917
Cfr13I GGNCC 2 cut(s) 64, 464
Csp6I GTAC 4 cut(s) 97, 183, 368, 977
CspCI CAANNNNNGTGG 2 cut(s) 897, 932
CviAII CATG 6 cut(s) 269, 389, 534, 610, 693, 938
CviQI GTAC 4 cut(s) 97, 183, 368, 977
DdeI CTNAG 4 cut(s) 57, 323, 719, 867
DpnI GATC 5 cut(s) 24, 252, 597, 725, 799
DpnII GATC 5 cut(s) 22, 250, 595, 723, 797
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco105I TACGTA 1 cut(s) 96
Eco130I CCWWGG 2 cut(s) 591, 946
Eco47I GGWCC 2 cut(s) 64, 464
EcoT14I CCWWGG 2 cut(s) 591, 946
ErhI CCWWGG 2 cut(s) 591, 946
FaeI CATG 6 cut(s) 272, 392, 537, 613, 696, 941
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FaqI GGGAC 1 cut(s) 430
FatI CATG 6 cut(s) 268, 388, 533, 609, 692, 937
FbaI TGATCA 1 cut(s) 250
Fnu4HI GCNGC 6 cut(s) 54, 167, 261, 402, 478, 531
FokI GGATG 5 cut(s) 161, 389, 406, 659, 749
Fsp4HI GCNGC 6 cut(s) 54, 167, 261, 402, 478, 531
FspBI CTAG 4 cut(s) 131, 264, 455, 525
GluI GCNGC 6 cut(s) 54, 167, 261, 402, 478, 531
GsuI CTGGAG 1 cut(s) 835
HapII CCGG 1 cut(s) 767
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 6 cut(s) 272, 392, 537, 613, 696, 941
HinfI GANTC 4 cut(s) 71, 89, 329, 614
HpaII CCGG 1 cut(s) 767
HphI GGTGA 3 cut(s) 257, 693, 725
Hpy166II GTNNAC 2 cut(s) 701, 887
Hpy188I TCNGA 4 cut(s) 464, 553, 843, 870
Hpy188III TCNNGA 2 cut(s) 455, 814
Hpy8I GTNNAC 2 cut(s) 701, 887
HpyAV CCTTC 3 cut(s) 338, 368, 682
HpyCH4III ACNGT 3 cut(s) 713, 964, 981
HpyCH4IV ACGT 4 cut(s) 39, 95, 241, 451
HpyF3I CTNAG 4 cut(s) 57, 323, 719, 867
HpySE526I ACGT 4 cut(s) 39, 95, 241, 451
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 6 cut(s) 272, 392, 537, 613, 696, 941
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 5 cut(s) 22, 250, 595, 723, 797
LmnI GCTCC 2 cut(s) 163, 474
LpnPI CCDG 9 cut(s) 63, 85, 208, 359, 399, 468, 621, 780, 799
Lsp1109I GCAGC 6 cut(s) 65, 153, 272, 388, 489, 517
LweI GCATC 3 cut(s) 847, 872, 945
MaeI CTAG 4 cut(s) 131, 264, 455, 525
MaeII ACGT 4 cut(s) 39, 95, 241, 451
MaeIII GTNAC 1 cut(s) 491
MalI GATC 5 cut(s) 24, 252, 597, 725, 799
MboI GATC 5 cut(s) 22, 250, 595, 723, 797
MboII GAAGA 7 cut(s) 34, 215, 392, 395, 513, 765, 802
MfeI CAATTG 2 cut(s) 833, 875
MflI RGATCY 2 cut(s) 595, 797
MluCI AATT 8 cut(s) 275, 300, 601, 630, 706, 762, 833, 875
MmeI TCCRAC 1 cut(s) 180
MnlI CCTC 7 cut(s) 52, 55, 111, 636, 714, 769, 810
MseI TTAA 3 cut(s) 519, 570, 1000
MslI CAYNNNNRTG 1 cut(s) 147
MspA1I CMGCKG 3 cut(s) 56, 77, 373
MspI CCGG 1 cut(s) 767
MspR9I CCNGG 1 cut(s) 768
MunI CAATTG 2 cut(s) 833, 875
NciI CCSGG 1 cut(s) 768
NdeII GATC 5 cut(s) 22, 250, 595, 723, 797
NheI GCTAGC 1 cut(s) 130
NlaIII CATG 6 cut(s) 272, 392, 537, 613, 696, 941
NlaIV GGNNCC 1 cut(s) 597
NmuCI GTSAC 1 cut(s) 491
NspI RCATGY 2 cut(s) 537, 696
PaeI GCATGC 2 cut(s) 537, 696
PfeI GAWTC 4 cut(s) 71, 89, 329, 614
PkrI GCNGC 6 cut(s) 55, 168, 262, 403, 479, 532
Ppu21I YACGTR 1 cut(s) 96
PspN4I GGNNCC 1 cut(s) 597
PspPI GGNCC 2 cut(s) 64, 464
PstI CTGCAG 1 cut(s) 55
PsuI RGATCY 2 cut(s) 595, 797
PvuII CAGCTG 3 cut(s) 56, 77, 373
RsaI GTAC 4 cut(s) 98, 184, 369, 978
RsaNI GTAC 4 cut(s) 97, 183, 368, 977
RseI CAYNNNNRTG 1 cut(s) 147
SaqAI TTAA 3 cut(s) 519, 570, 1000
SatI GCNGC 6 cut(s) 54, 167, 261, 402, 478, 531
Sau3AI GATC 5 cut(s) 22, 250, 595, 723, 797
Sau96I GGNCC 2 cut(s) 64, 464
ScrFI CCNGG 1 cut(s) 768
SfaNI GCATC 3 cut(s) 847, 872, 945
SfcI CTRYAG 2 cut(s) 51, 157
SinI GGWCC 2 cut(s) 64, 464
SmiMI CAYNNNNRTG 1 cut(s) 147
SnaBI TACGTA 1 cut(s) 96
SphI GCATGC 2 cut(s) 537, 696
Sse9I AATT 8 cut(s) 275, 300, 601, 630, 706, 762, 833, 875
SsiI CCGC 1 cut(s) 734
SspMI CTAG 4 cut(s) 131, 264, 455, 525
StyD4I CCNGG 1 cut(s) 766
StyI CCWWGG 2 cut(s) 591, 946
TaaI ACNGT 3 cut(s) 713, 964, 981
TaiI ACGT 4 cut(s) 42, 98, 244, 454
TaqI TCGA 2 cut(s) 112, 361
TasI AATT 8 cut(s) 275, 300, 601, 630, 706, 762, 833, 875
TfiI GAWTC 4 cut(s) 71, 89, 329, 614
Tru1I TTAA 3 cut(s) 519, 570, 1000
Tru9I TTAA 3 cut(s) 519, 570, 1000
TscAI CASTG 1 cut(s) 967
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 6 cut(s) 53, 166, 260, 401, 477, 530
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 7 cut(s) 165, 393, 462, 527, 570, 753, 926
TspGWI ACGGA 1 cut(s) 904
TspRI CASTG 1 cut(s) 967
VpaK11BI GGWCC 2 cut(s) 64, 464
XapI RAATTY 2 cut(s) 300, 601
XbaI TCTAGA 1 cut(s) 454
XceI RCATGY 2 cut(s) 537, 696
XcmI CCANNNNNNNNNTGG 2 cut(s) 196, 894
XspI CTAG 4 cut(s) 131, 264, 455, 525
ZraI GACGTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.