MD16G1258200.v1.1

Syntaxin 6, N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009458
Physical Location & Seq
Reverse (-)
30382358 .. 30387638
5281 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1258200.v1.1.491

Sequence Viewer

Length: 1002 bp
ATGATTGTGGCAAGCAGTTTTGATCTTTGGAAAAAAGACGTCTTCTTTCCAGCAGCTGAGGAGGTCCAAGAATCAGCTGACATAATGGAATCTACATACAGGGCATGGGTTCGACAGAGGAGTGAAAGGCTAGCACCAGAACATTTGGATGAACTCTGTAGGGAGCTGCAAACCGCTTTGGGTACTGCCAAATGGCAGTTGGAAGAGTTTGAGAAGGCTGTCAGGTTGAGCTATGGACAATGTGGTGATGATCATACAGCAGCTAGACATGGACAATTTCTTGCTGCTATTGAAAACCAAATTTCTCGTGTTGAAACAACACTAAGGGAATCATTTAGTATGGAAGGAAAGAAACCCCTTCAATGGGTACACATGGATGAAGAAGAACAAGATGATTTTGCTGCATTTCTCTTTGGGACATCCCAAAGTAAGCAAACTGCCAAAAATGAACGTGTAGAACTCAGGCATTGGGTGAAAAGCTCCGAGGAAAGTGACATTACAAGAAAAGACAGGAACCTTAAATATGGTGCTGCCTGCAATGGAGACATTTCTGATGAAATAAAGGGTGTTAAAGATGTTATTACTATTTCCAAGGATCCAAATTTTGTCATGGAATTGAAAGGAAAAGAAATCGCAGAAATGAAGGATGATAAAATTTATACAAGGAAGACATGCAGTTCACCAAATTACAGGGAGCTGAGGATCGTAATAGCAGATGAAGTTGAACAGAGAAAAAATCTAATTCCAGGGTATGAGGATACACCCAAAGAAAAAGGATTCAAACTTGTCTTCGGGAAGCAAAGGTTGCATCAGGCAACTAAAGCAATTAATTTGTTCAACCAGATGTACAGTCGAGTTGGTGCATCTCAGAAGCAATTGCAAAGCCCACGGAAGCAATGGCAAGCACCTCCACGCCTGAACAATCGTTGTTCAGTTCAAGTTACCCTTGGTTTAATGCTCACTGTTTTTCTACTTGTACCGTTTGTATTTTATTCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syntaxin-6_N

Protein Analysis

334

Amino Acids

38.39

Weight (kDa)

6.64

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
STX6_10_61_N PF09177 13 - 105 3.1e-18 Syntaxin 6/10/61, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 42
AciI CCGC 1 cut(s) 174
AclWI GGATC 3 cut(s) 590, 603, 710
AcsI RAATTY 3 cut(s) 300, 601, 654
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 4 cut(s) 184, 369, 848, 978
AfiI CCNNNNNNNGG 2 cut(s) 363, 364
AflIII ACRYGT 1 cut(s) 451
AgsI TTSAA 9 cut(s) 293, 314, 362, 619, 725, 781, 838, 938, 996
AjnI CCWGG 1 cut(s) 745
AluBI AGCT 7 cut(s) 56, 77, 166, 231, 263, 480, 697
AluI AGCT 7 cut(s) 56, 77, 166, 231, 263, 480, 697
Alw26I GTCTC 1 cut(s) 537
AlwI GGATC 3 cut(s) 590, 603, 710
AlwNI CAGNNNCTG 1 cut(s) 56
ApeKI GCWGC 6 cut(s) 53, 166, 260, 284, 401, 530
ApoI RAATTY 3 cut(s) 300, 601, 654
AseI ATTAAT 1 cut(s) 828
AspS9I GGNCC 1 cut(s) 64
AsuHPI GGTGA 3 cut(s) 257, 484, 672
AsuNHI GCTAGC 1 cut(s) 130
AvaII GGWCC 1 cut(s) 64
BamHI GGATCC 1 cut(s) 595
BauI CACGAG 1 cut(s) 306
BbsI GAAGAC 3 cut(s) 34, 674, 781
BbvCI CCTCAGC 2 cut(s) 57, 698
BbvI GCAGC 6 cut(s) 65, 153, 271, 272, 388, 517
BciT130I CCWGG 1 cut(s) 747
BciVI GTATCC 1 cut(s) 751
BclI TGATCA 1 cut(s) 250
BcoDI GTCTC 1 cut(s) 537
BfaI CTAG 2 cut(s) 131, 264
BfmI CTRYAG 1 cut(s) 157
BfuI GTATCC 1 cut(s) 751
BisI GCNGC 6 cut(s) 54, 167, 261, 285, 402, 531
BlsI GCNGC 6 cut(s) 55, 168, 262, 286, 403, 532
Bme1390I CCNGG 1 cut(s) 747
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 2 cut(s) 515, 597
BmrFI CCNGG 1 cut(s) 747
BmsI GCATC 2 cut(s) 817, 872
BmtI GCTAGC 1 cut(s) 134
BpiI GAAGAC 3 cut(s) 34, 674, 781
Bpu10I CCTNAGC 2 cut(s) 57, 698
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 5 cut(s) 483, 591, 746, 887, 946
Bsc4I CCNNNNNNNGG 2 cut(s) 363, 364
Bse3DI GCAATG 2 cut(s) 544, 902
BseBI CCWGG 1 cut(s) 747
BseDI CCNNGG 5 cut(s) 483, 591, 746, 887, 946
BseGI GGATG 4 cut(s) 154, 382, 419, 652
BseLI CCNNNNNNNGG 2 cut(s) 363, 364
BseMI GCAATG 2 cut(s) 544, 902
BseMII CTCAG 4 cut(s) 48, 475, 689, 881
BseRI GAGGAG 2 cut(s) 74, 133
BseXI GCAGC 6 cut(s) 65, 153, 271, 272, 388, 517
BslFI GGGAC 1 cut(s) 430
BslI CCNNNNNNNGG 2 cut(s) 363, 364
BsmAI GTCTC 1 cut(s) 537
BsmFI GGGAC 1 cut(s) 430
Bsp1407I TGTACA 1 cut(s) 846
Bsp143I GATC 4 cut(s) 22, 250, 595, 702
BspACI CCGC 1 cut(s) 174
BspCNI CTCAG 4 cut(s) 49, 474, 690, 880
BspLI GGNNCC 2 cut(s) 515, 597
BspOI GCTAGC 1 cut(s) 134
BspPI GGATC 3 cut(s) 590, 603, 710
BsrDI GCAATG 2 cut(s) 544, 902
BsrGI TGTACA 1 cut(s) 846
BssECI CCNNGG 5 cut(s) 483, 591, 746, 887, 946
BssMI GATC 4 cut(s) 22, 250, 595, 702
BssNI GRCGYC 1 cut(s) 39
BssSI CACGAG 1 cut(s) 306
BssT1I CCWWGG 2 cut(s) 591, 946
Bst2BI CACGAG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 747
Bst4CI ACNGT 3 cut(s) 851, 964, 981
Bst6I CTCTTC 1 cut(s) 198
BstACI GRCGYC 1 cut(s) 39
BstAPI GCANNNNNTGC 1 cut(s) 805
BstAUI TGTACA 1 cut(s) 846
BstC8I GCNNGC 4 cut(s) 13, 132, 535, 903
BstDEI CTNAG 5 cut(s) 57, 323, 461, 698, 867
BstDSI CCRYGG 1 cut(s) 887
BstF5I GGATG 4 cut(s) 154, 382, 419, 652
BstKTI GATC 4 cut(s) 25, 253, 598, 705
BstMAI GTCTC 1 cut(s) 537
BstMBI GATC 4 cut(s) 22, 250, 595, 702
BstMWI GCNNNNNNNGC 2 cut(s) 805, 821
BstNI CCWGG 1 cut(s) 747
BstNSI RCATGY 1 cut(s) 675
BstSCI CCNGG 1 cut(s) 745
BstSFI CTRYAG 1 cut(s) 157
BstV1I GCAGC 6 cut(s) 65, 153, 271, 272, 388, 517
BstV2I GAAGAC 3 cut(s) 34, 674, 781
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
BsuI GTATCC 1 cut(s) 751
BtgI CCRYGG 1 cut(s) 887
BtsCI GGATG 4 cut(s) 154, 382, 419, 652
BtsIMutI CAGTG 1 cut(s) 960
Cac8I GCNNGC 4 cut(s) 13, 132, 535, 903
CaiI CAGNNNCTG 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 64
Csp6I GTAC 4 cut(s) 183, 368, 847, 977
CviAII CATG 5 cut(s) 105, 269, 373, 610, 672
CviQI GTAC 4 cut(s) 183, 368, 847, 977
DdeI CTNAG 5 cut(s) 57, 323, 461, 698, 867
DpnI GATC 4 cut(s) 24, 252, 597, 704
DpnII GATC 4 cut(s) 22, 250, 595, 702
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco130I CCWWGG 2 cut(s) 591, 946
Eco47I GGWCC 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 745
EcoT14I CCWWGG 2 cut(s) 591, 946
ErhI CCWWGG 2 cut(s) 591, 946
FaeI CATG 5 cut(s) 108, 272, 376, 613, 675
FalI AAGNNNNNCTT 2 cut(s) 930, 962
FaqI GGGAC 1 cut(s) 430
FatI CATG 5 cut(s) 104, 268, 372, 609, 671
FbaI TGATCA 1 cut(s) 250
Fnu4HI GCNGC 6 cut(s) 54, 167, 261, 285, 402, 531
FokI GGATG 4 cut(s) 161, 389, 406, 659
Fsp4HI GCNGC 6 cut(s) 54, 167, 261, 285, 402, 531
FspBI CTAG 2 cut(s) 131, 264
GluI GCNGC 6 cut(s) 54, 167, 261, 285, 402, 531
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 5 cut(s) 108, 272, 376, 613, 675
HinfI GANTC 4 cut(s) 71, 89, 329, 777
HphI GGTGA 3 cut(s) 257, 484, 672
Hpy166II GTNNAC 2 cut(s) 370, 680
Hpy188I TCNGA 3 cut(s) 484, 553, 870
Hpy188III TCNNGA 1 cut(s) 793
Hpy8I GTNNAC 2 cut(s) 370, 680
HpyAV CCTTC 4 cut(s) 208, 338, 368, 637
HpyCH4III ACNGT 3 cut(s) 851, 964, 981
HpyCH4IV ACGT 2 cut(s) 39, 451
HpyCH4V TGCA 7 cut(s) 169, 404, 537, 675, 808, 863, 880
HpyF10VI GCNNNNNNNGC 2 cut(s) 805, 821
HpyF3I CTNAG 5 cut(s) 57, 323, 461, 698, 867
HpySE526I ACGT 2 cut(s) 39, 451
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 5 cut(s) 108, 272, 376, 613, 675
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 4 cut(s) 22, 250, 595, 702
LmnI GCTCC 3 cut(s) 163, 485, 694
Lsp1109I GCAGC 6 cut(s) 65, 153, 271, 272, 388, 517
LweI GCATC 2 cut(s) 817, 872
MaeI CTAG 2 cut(s) 131, 264
MaeII ACGT 2 cut(s) 39, 451
MaeIII GTNAC 2 cut(s) 491, 940
MalI GATC 4 cut(s) 24, 252, 597, 704
MboI GATC 4 cut(s) 22, 250, 595, 702
MboII GAAGA 6 cut(s) 34, 215, 392, 395, 679, 781
MfeI CAATTG 1 cut(s) 875
MflI RGATCY 1 cut(s) 595
MmeI TCCRAC 1 cut(s) 180
MnlI CCTC 7 cut(s) 52, 55, 111, 478, 693, 748, 918
MseI TTAA 5 cut(s) 519, 570, 828, 953, 1000
MslI CAYNNNNRTG 2 cut(s) 147, 375
MspA1I CMGCKG 2 cut(s) 56, 77
MspR9I CCNGG 1 cut(s) 747
MunI CAATTG 1 cut(s) 875
MvaI CCWGG 1 cut(s) 747
MwoI GCNNNNNNNGC 2 cut(s) 805, 821
NdeII GATC 4 cut(s) 22, 250, 595, 702
NheI GCTAGC 1 cut(s) 130
NlaIII CATG 5 cut(s) 108, 272, 376, 613, 675
NlaIV GGNNCC 2 cut(s) 515, 597
NmuCI GTSAC 1 cut(s) 491
NspI RCATGY 1 cut(s) 675
PfeI GAWTC 4 cut(s) 71, 89, 329, 777
PkrI GCNGC 6 cut(s) 55, 168, 262, 286, 403, 532
PshBI ATTAAT 1 cut(s) 828
Psp6I CCWGG 1 cut(s) 745
PspGI CCWGG 1 cut(s) 745
PspN4I GGNNCC 2 cut(s) 515, 597
PspPI GGNCC 1 cut(s) 64
PstNI CAGNNNCTG 1 cut(s) 56
PsuI RGATCY 1 cut(s) 595
PvuII CAGCTG 2 cut(s) 56, 77
RsaI GTAC 4 cut(s) 184, 369, 848, 978
RsaNI GTAC 4 cut(s) 183, 368, 847, 977
RseI CAYNNNNRTG 2 cut(s) 147, 375
SaqAI TTAA 5 cut(s) 519, 570, 828, 953, 1000
SatI GCNGC 6 cut(s) 54, 167, 261, 285, 402, 531
Sau3AI GATC 4 cut(s) 22, 250, 595, 702
Sau96I GGNCC 1 cut(s) 64
ScrFI CCNGG 1 cut(s) 747
SfaNI GCATC 2 cut(s) 817, 872
SfcI CTRYAG 1 cut(s) 157
SinI GGWCC 1 cut(s) 64
SmiMI CAYNNNNRTG 2 cut(s) 147, 375
SsiI CCGC 1 cut(s) 174
SspMI CTAG 2 cut(s) 131, 264
StyD4I CCNGG 1 cut(s) 745
StyI CCWWGG 2 cut(s) 591, 946
TaaI ACNGT 3 cut(s) 851, 964, 981
TaiI ACGT 2 cut(s) 42, 454
TaqI TCGA 2 cut(s) 112, 853
TatI WGTACW 1 cut(s) 846
TfiI GAWTC 4 cut(s) 71, 89, 329, 777
Tru1I TTAA 5 cut(s) 519, 570, 828, 953, 1000
Tru9I TTAA 5 cut(s) 519, 570, 828, 953, 1000
TscAI CASTG 1 cut(s) 967
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 6 cut(s) 53, 166, 260, 284, 401, 530
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 6 cut(s) 165, 393, 462, 570, 656, 732
TspGWI ACGGA 1 cut(s) 904
TspRI CASTG 1 cut(s) 967
VpaK11BI GGWCC 1 cut(s) 64
VspI ATTAAT 1 cut(s) 828
XapI RAATTY 3 cut(s) 300, 601, 654
XceI RCATGY 1 cut(s) 675
XcmI CCANNNNNNNNNTGG 2 cut(s) 196, 894
XspI CTAG 2 cut(s) 131, 264
ZraI GACGTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.