MD14G1010100.v1.1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012658
Physical Location & Seq
Reverse (-)
975219 .. 976015
797 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1010100.v1.1.491

Sequence Viewer

Length: 363 bp
ATGCCAATAGCCATCATCGCCCACCCTCTCTCCCCTCCTTTTATTTTTCTCCAGTCTGCAGTGCTGTGTAGATCCATTGAGTGCTTCATAAGAAGCAAAGGCATGGCGCACAAGTGTAATTTTATGACACAACTAGTTATCTTTCTATGTTTTACATATGTCCTATCATCAATAGCTGCAGCTCCTACGACAAGAAGTCGGAAGATAAGTAAGGAAGGCCTACCGAGTCAGAATTTGTTGGATGAAGGTGTCCTTGATTTGGGAGAAGGATTTGTGGAGGGGCGGATGGATCTGGAAACCAATGATTATTCAGGGGTTGGAGCAAATCCGGTGCATGATCCAAAACCCCCAGGAAGAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

13.11

Weight (kDa)

6.81

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 283
AclWI GGATC 3 cut(s) 66, 297, 332
AcsI RAATTY 2 cut(s) 232, 357
AfiI CCNNNNNNNGG 1 cut(s) 259
AhdI GACNNNNNGTC 1 cut(s) 195
AhlI ACTAGT 1 cut(s) 133
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 2 cut(s) 176, 182
AluI AGCT 2 cut(s) 176, 182
AlwI GGATC 3 cut(s) 66, 297, 332
AoxI GGCC 1 cut(s) 217
ApeKI GCWGC 2 cut(s) 176, 179
ApoI RAATTY 2 cut(s) 232, 357
AspLEI GCGC 1 cut(s) 109
BbvI GCAGC 2 cut(s) 163, 191
BccI CCATC 2 cut(s) 20, 280
BciT130I CCWGG 1 cut(s) 351
BcuI ACTAGT 1 cut(s) 133
BfaI CTAG 1 cut(s) 134
BfmI CTRYAG 2 cut(s) 57, 177
BisI GCNGC 2 cut(s) 177, 180
BlsI GCNGC 2 cut(s) 178, 181
Bme1390I CCNGG 1 cut(s) 351
BmeRI GACNNNNNGTC 1 cut(s) 195
BmrFI CCNGG 1 cut(s) 351
BpmI CTGGAG 1 cut(s) 35
BsaJI CCNNGG 1 cut(s) 349
BsaWI WCCGGW 1 cut(s) 328
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse1I ACTGG 1 cut(s) 52
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 1 cut(s) 349
BseGI GGATG 2 cut(s) 247, 291
BseLI CCNNNNNNNGG 1 cut(s) 259
BseNI ACTGG 1 cut(s) 52
BseXI GCAGC 2 cut(s) 163, 191
BshFI GGCC 1 cut(s) 219
BsiSI CCGG 1 cut(s) 329
BslI CCNNNNNNNGG 1 cut(s) 259
BsnI GGCC 1 cut(s) 219
Bsp143I GATC 3 cut(s) 71, 289, 337
BspACI CCGC 1 cut(s) 283
BspANI GGCC 1 cut(s) 219
BspMAI CTGCAG 2 cut(s) 61, 181
BspPI GGATC 3 cut(s) 66, 297, 332
BsrI ACTGG 1 cut(s) 52
BssECI CCNNGG 1 cut(s) 349
BssMI GATC 3 cut(s) 71, 289, 337
Bst2UI CCWGG 1 cut(s) 351
BstF5I GGATG 2 cut(s) 247, 291
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 3 cut(s) 74, 292, 340
BstMBI GATC 3 cut(s) 71, 289, 337
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNI CCWGG 1 cut(s) 351
BstSCI CCNGG 1 cut(s) 349
BstSFI CTRYAG 2 cut(s) 57, 177
BstV1I GCAGC 2 cut(s) 163, 191
BstX2I RGATCY 2 cut(s) 71, 289
BstYI RGATCY 2 cut(s) 71, 289
BsuRI GGCC 1 cut(s) 219
BtsCI GGATG 2 cut(s) 247, 291
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 1 cut(s) 66
CfoI GCGC 1 cut(s) 109
CviAII CATG 2 cut(s) 103, 335
CviJI RGCY 4 cut(s) 11, 176, 182, 219
CviKI_1 RGCY 4 cut(s) 11, 176, 182, 219
DpnI GATC 3 cut(s) 73, 291, 339
DpnII GATC 3 cut(s) 71, 289, 337
DriI GACNNNNNGTC 1 cut(s) 195
Eam1105I GACNNNNNGTC 1 cut(s) 195
EciI GGCGGA 1 cut(s) 298
Eco147I AGGCCT 1 cut(s) 219
EcoRII CCWGG 1 cut(s) 349
FaeI CATG 2 cut(s) 106, 338
FaiI YATR 7 cut(s) 89, 104, 125, 148, 157, 159, 336
FalI AAGNNNNNCTT 2 cut(s) 237, 269
FatI CATG 2 cut(s) 102, 334
FauNDI CATATG 1 cut(s) 157
Fnu4HI GCNGC 2 cut(s) 177, 180
FokI GGATG 2 cut(s) 254, 298
Fsp4HI GCNGC 2 cut(s) 177, 180
FspBI CTAG 1 cut(s) 134
GlaI GCGC 1 cut(s) 108
GluI GCNGC 2 cut(s) 177, 180
GsuI CTGGAG 1 cut(s) 35
HaeIII GGCC 1 cut(s) 219
HapII CCGG 1 cut(s) 329
HhaI GCGC 1 cut(s) 109
Hin1II CATG 2 cut(s) 106, 338
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HinfI GANTC 1 cut(s) 226
HpaII CCGG 1 cut(s) 329
Hpy188I TCNGA 2 cut(s) 201, 231
Hpy188III TCNNGA 1 cut(s) 293
HpyAV CCTTC 3 cut(s) 209, 239, 260
HpyCH4V TGCA 3 cut(s) 59, 179, 334
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
Hsp92II CATG 2 cut(s) 106, 338
HspAI GCGC 1 cut(s) 107
Kzo9I GATC 3 cut(s) 71, 289, 337
LmnI GCTCC 2 cut(s) 187, 320
LpnPI CCDG 5 cut(s) 65, 278, 297, 336, 342
Lsp1109I GCAGC 2 cut(s) 163, 191
MaeI CTAG 1 cut(s) 134
MalI GATC 3 cut(s) 73, 291, 339
MboI GATC 3 cut(s) 71, 289, 337
MboII GAAGA 1 cut(s) 214
MflI RGATCY 2 cut(s) 71, 289
MluCI AATT 3 cut(s) 118, 232, 357
MlyI GAGTC 1 cut(s) 235
MmeI TCCRAC 3 cut(s) 179, 219, 298
MnlI CCTC 3 cut(s) 36, 45, 271
MspI CCGG 1 cut(s) 329
MspR9I CCNGG 1 cut(s) 351
MvaI CCWGG 1 cut(s) 351
MwoI GCNNNNNNNGC 1 cut(s) 17
NdeI CATATG 1 cut(s) 157
NdeII GATC 3 cut(s) 71, 289, 337
NlaIII CATG 2 cut(s) 106, 338
PceI AGGCCT 1 cut(s) 219
PkrI GCNGC 2 cut(s) 178, 181
PleI GAGTC 1 cut(s) 234
PpsI GAGTC 1 cut(s) 234
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PstI CTGCAG 2 cut(s) 61, 181
PsuI RGATCY 2 cut(s) 71, 289
SatI GCNGC 2 cut(s) 177, 180
Sau3AI GATC 3 cut(s) 71, 289, 337
SchI GAGTC 1 cut(s) 235
ScrFI CCNGG 1 cut(s) 351
SetI ASST 3 cut(s) 178, 184, 250
SfcI CTRYAG 2 cut(s) 57, 177
SpeI ACTAGT 1 cut(s) 133
Sse9I AATT 3 cut(s) 118, 232, 357
SseBI AGGCCT 1 cut(s) 219
SsiI CCGC 1 cut(s) 283
SspMI CTAG 1 cut(s) 134
StuI AGGCCT 1 cut(s) 219
StyD4I CCNGG 1 cut(s) 349
TasI AATT 3 cut(s) 118, 232, 357
TscAI CASTG 1 cut(s) 66
TseI GCWGC 2 cut(s) 176, 179
TspDTI ATGAA 2 cut(s) 76, 258
TspRI CASTG 1 cut(s) 66
XapI RAATTY 2 cut(s) 232, 357
XspI CTAG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.