pycom12g00980

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012658
Physical Location & Seq
Forward (+)
833818 .. 835049
1232 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g00980.1

Sequence Viewer

Length: 285 bp
ATGCTGCAGAAGCCTTTCCTTAGACTGCTGCTGGTACTCTTATTGGTTTTCTCTCATGTTCTATTTTTGTCTGCGGTTCCTGCAACCAGGAGCCTCAAATCAGAGGAGGAAGACTTATCGGTCCAAGAATCTCTGGGTCTAGTGGAGTTGGTGAATGGAGAGGAGGTGTTTGATGTGGGTGGGGGAGAGGCGTTTATTGAAGGAAGGGTGACTATAGAAAGTGAAGACTATCCGGGAACAGGAGCAAACAGCCATCATGACCCAAGAACCCCTGGAAAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

95

Amino Acids

10.2

Weight (kDa)

4.65

Isoelectric Point (pI)

54.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AciI CCGC 1 cut(s) 74
AfaI GTAC 1 cut(s) 36
AfiI CCNNNNNNNGG 1 cut(s) 239
AgsI TTSAA 1 cut(s) 200
AjnI CCWGG 2 cut(s) 86, 271
AluBI AGCT 1 cut(s) 281
AluI AGCT 1 cut(s) 281
ApeKI GCWGC 2 cut(s) 4, 28
Asp700I GAANNNNTTC 1 cut(s) 14
AspS9I GGNCC 1 cut(s) 121
AsuC2I CCSGG 1 cut(s) 234
AsuHPI GGTGA 2 cut(s) 163, 220
AvaII GGWCC 1 cut(s) 121
BbsI GAAGAC 2 cut(s) 117, 231
BbvI GCAGC 1 cut(s) 15
BccI CCATC 1 cut(s) 261
BciT130I CCWGG 2 cut(s) 88, 273
BcnI CCSGG 1 cut(s) 234
BfaI CTAG 1 cut(s) 140
BfmI CTRYAG 2 cut(s) 5, 213
BisI GCNGC 2 cut(s) 5, 29
BlsI GCNGC 2 cut(s) 6, 30
Bme1390I CCNGG 3 cut(s) 88, 234, 273
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 121
BmiI GGNNCC 2 cut(s) 78, 92
BmrFI CCNGG 3 cut(s) 88, 234, 273
BpiI GAAGAC 2 cut(s) 117, 231
BpuMI CCSGG 1 cut(s) 234
BsaJI CCNNGG 1 cut(s) 271
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 1 cut(s) 239
BseBI CCWGG 2 cut(s) 88, 273
BseDI CCNNGG 1 cut(s) 271
BseLI CCNNNNNNNGG 1 cut(s) 239
BseRI GAGGAG 2 cut(s) 119, 176
BseXI GCAGC 1 cut(s) 15
BsiSI CCGG 1 cut(s) 233
BslI CCNNNNNNNGG 1 cut(s) 239
BspACI CCGC 1 cut(s) 74
BspHI TCATGA 1 cut(s) 256
BspLI GGNNCC 2 cut(s) 78, 92
BspMAI CTGCAG 1 cut(s) 9
BssECI CCNNGG 1 cut(s) 271
Bst2UI CCWGG 2 cut(s) 88, 273
BstDEI CTNAG 1 cut(s) 20
BstMWI GCNNNNNNNGC 2 cut(s) 10, 80
BstNI CCWGG 2 cut(s) 88, 273
BstSCI CCNGG 3 cut(s) 86, 232, 271
BstSFI CTRYAG 2 cut(s) 5, 213
BstV1I GCAGC 1 cut(s) 15
BstV2I GAAGAC 2 cut(s) 117, 231
CciI TCATGA 1 cut(s) 256
Cfr13I GGNCC 1 cut(s) 121
Csp6I GTAC 1 cut(s) 35
CviAII CATG 2 cut(s) 56, 257
CviJI RGCY 4 cut(s) 13, 93, 252, 281
CviKI_1 RGCY 4 cut(s) 13, 93, 252, 281
CviQI GTAC 1 cut(s) 35
DdeI CTNAG 1 cut(s) 20
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
Eco47I GGWCC 1 cut(s) 121
EcoRII CCWGG 2 cut(s) 86, 271
FaeI CATG 2 cut(s) 59, 260
FaiI YATR 3 cut(s) 57, 215, 258
FatI CATG 2 cut(s) 55, 256
Fnu4HI GCNGC 2 cut(s) 5, 29
Fsp4HI GCNGC 2 cut(s) 5, 29
FspBI CTAG 1 cut(s) 140
GluI GCNGC 2 cut(s) 5, 29
HapII CCGG 1 cut(s) 233
Hin1II CATG 2 cut(s) 59, 260
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 1 cut(s) 128
HpaII CCGG 1 cut(s) 233
HphI GGTGA 2 cut(s) 163, 220
Hpy188I TCNGA 1 cut(s) 103
Hpy188III TCNNGA 1 cut(s) 257
HpyAV CCTTC 2 cut(s) 194, 198
HpyCH4V TGCA 2 cut(s) 7, 83
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 80
HpyF3I CTNAG 1 cut(s) 20
Hsp92II CATG 2 cut(s) 59, 260
LmnI GCTCC 2 cut(s) 90, 242
LpnPI CCDG 8 cut(s) 17, 73, 93, 100, 119, 225, 246, 258
Lsp1109I GCAGC 1 cut(s) 15
MaeI CTAG 1 cut(s) 140
MaeIII GTNAC 1 cut(s) 208
MboII GAAGA 2 cut(s) 122, 236
MnlI CCTC 6 cut(s) 97, 100, 104, 154, 157, 181
MroXI GAANNNNTTC 1 cut(s) 14
MseI TTAA 1 cut(s) 283
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 3 cut(s) 88, 234, 273
MvaI CCWGG 2 cut(s) 88, 273
MwoI GCNNNNNNNGC 2 cut(s) 10, 80
NciI CCSGG 1 cut(s) 234
NlaIII CATG 2 cut(s) 59, 260
NlaIV GGNNCC 2 cut(s) 78, 92
NmuCI GTSAC 1 cut(s) 208
PagI TCATGA 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 14
PfeI GAWTC 1 cut(s) 128
PfoI TCCNGGA 1 cut(s) 232
PkrI GCNGC 2 cut(s) 6, 30
Psp6I CCWGG 2 cut(s) 86, 271
PspGI CCWGG 2 cut(s) 86, 271
PspN4I GGNNCC 2 cut(s) 78, 92
PspPI GGNCC 1 cut(s) 121
PstI CTGCAG 1 cut(s) 9
RsaI GTAC 1 cut(s) 36
RsaNI GTAC 1 cut(s) 35
SaqAI TTAA 1 cut(s) 283
SatI GCNGC 2 cut(s) 5, 29
Sau96I GGNCC 1 cut(s) 121
ScrFI CCNGG 3 cut(s) 88, 234, 273
SetI ASST 2 cut(s) 168, 283
SfcI CTRYAG 2 cut(s) 5, 213
SinI GGWCC 1 cut(s) 121
SsiI CCGC 1 cut(s) 74
SspMI CTAG 1 cut(s) 140
StyD4I CCNGG 3 cut(s) 86, 232, 271
TaqII GACCGA 1 cut(s) 109
TfiI GAWTC 1 cut(s) 128
Tru1I TTAA 1 cut(s) 283
Tru9I TTAA 1 cut(s) 283
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 2 cut(s) 4, 28
Tsp45I GTSAC 1 cut(s) 208
VpaK11BI GGWCC 1 cut(s) 121
XmnI GAANNNNTTC 1 cut(s) 14
XspI CTAG 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.