Rorug03G0278000

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0012658
Physical Location & Seq
Reverse (-)
27528060 .. 27534429
6370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0278000.1

Sequence Viewer

Length: 1713 bp
ATGTCTACCCAATGCTTAGCGAGCAGCGGTAGCCCTACTGGTCAAACTTCACATCTGAATGTTAAAAGACTGACAGCAAATTACAAGCCAAGTATTTGGAGTCACGATTTTCTTCAGTCATTACAAATTGATGATCGTAGTCATGAAGAAGTCTACAAAGATAATAGGTGGAAGTCGATCGAGGAGGATCTGAAAAATATGATTGATGCAGAGTCACTAGAGACTACACTTGAATTGATTGATGATATCCAACGATTAGGTTTGGGACATCGATTCGAGACCAGTATAACAGAAGCCCTTGATAAAGTTTTGAAGAATCTTGACCAAATAACAGACGAGGATACTCCAAGTCTGCACCTCACTGCTCTCAGCTTTAGGCTCCTTAGACAACATGGGTTTCAAGTCTCCCAAGATGTTTTCAAAATTTTCACGGACTGCGACGACAGCTTCAAGGATTGCCTTTGCAAAGATGTCAAAGGAATGTTGAGTCTATACGAAGCTTCATTCCTTGGTATTGAAGGAGAAACCCTCTTGGATGAGGCCCTTGCATTCACCAGCATGCACCTAAAGAACCCCAGCAGATTGCATGTTACTCAAGGCAAAGGTGTATTAGAGCAAGTGAGTCATGCATTGGAGATGCCATTGCACCATAGAATGCAAAGACTAGAGGCTAGATGGTATATTGAGGCATACAGTAAAAAGGCAGATGCAAATAAGGTGCTACTTGAATTTGCCAAGCTAGATTACAATGTGGTGCAACAAACATACCAAAGAGATCTTAAAGACATTTCAAGGTGGTGGAAGGATACGGGGTTAGCAAAGAAGTTATCTTTCAGCAGAGACAGACTGATGGAGTTGTTCTTTTGGTCAGTTGGTATAGTGTTTGAACCTCAACTCAGCAATCTTAGAAAAGGGATAACTAAAGTCAGTGCTCTAATAACCACAATTGATGATGTCTATGATGTTTATGGTGCTTTGGATGAACTAGAGCTATTCACATCTGTTGTTGAAAGATGGGATGTGAATGCTGTGGAGATTCTTTCAGACTACCAACTGAAGCTCTGTTTCCTTGCTCTCTATAACACTGTGAATGAATTGACTTATGAAACTTTGAAGGAGCAAGGAGTGAACATCCTTCCTTACGTGAGAAAAGCTTGGGCAGATATGTGCAAAACTTGGTTAACGGAGGCAGAGTGGCGTCACAACAAGTACACACCTACATTTGAAGAATATCTTGCCAATGCATGGATATCAGTGTCTGGGGTGGTCATTCTAGTCCATACCTACTTTCTACTTAATCAAAATATCTTAGATGAAGCACTTAAATGCTTAGAGAATCACCATGATCTCTTGCGTTGGCCGTCTCTTATTTTTCGGCTCTCAAATGATTTGGTTACTTCGAAGGCAGAACTAGAGAGAGGTGAAACTGCAACTTCAATATCCTGCATTAGACGTGATGGTGTTGTTTCTGATGAGGAATCTGCTCGCAATTATATTAGTAATTTGATTGAGAATAGTTGGAAGAAGATGAACAAAGATGGACTACTATTTGGTACTAGTAGAGCTTCTTCTCCATTCACAAAGGAATTTGTAGCAGCAGCAACGAACCTTGCTCGAATTGCCCAATGCATTTACCAATATGGAGACGGGATTGGTGCCCCAGACAAAAGAGTAAAGAACCAGATCCTAGCAGTGATTGTACAACCCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

570

Amino Acids

65.37

Weight (kDa)

5.44

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 32 - 211 2.9e-50 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 268 - 509 2e-84 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 308 - 509 5.7e-56 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1655
AccB7I CCANNNNNTGG 1 cut(s) 1245
AccI GTMKAC 2 cut(s) 5, 153
AciI CCGC 1 cut(s) 27
AclWI GGATC 2 cut(s) 195, 1678
AcoI YGGCCR 1 cut(s) 1358
AcsI RAATTY 3 cut(s) 423, 728, 1586
AcuI CTGAAG 2 cut(s) 98, 1076
AcyI GRCGYC 1 cut(s) 1198
AfaI GTAC 3 cut(s) 1211, 1555, 1701
AfiI CCNNNNNNNGG 1 cut(s) 1245
AhlI ACTAGT 1 cut(s) 1556
AjiI CACGTC 1 cut(s) 1454
AluBI AGCT 8 cut(s) 372, 447, 500, 739, 991, 1060, 1154, 1565
AluI AGCT 8 cut(s) 372, 447, 500, 739, 991, 1060, 1154, 1565
Alw21I GWGCWC 1 cut(s) 934
Alw26I GTCTC 6 cut(s) 215, 272, 409, 834, 1368, 1638
AlwI GGATC 2 cut(s) 195, 1678
AlwNI CAGNNNCTG 1 cut(s) 1259
AoxI GGCC 2 cut(s) 540, 1358
ApeKI GCWGC 3 cut(s) 24, 1595, 1598
ApoI RAATTY 3 cut(s) 423, 728, 1586
AspS9I GGNCC 1 cut(s) 541
AsuHPI GGTGA 3 cut(s) 544, 1331, 1433
AsuII TTCGAA 1 cut(s) 1400
BaeGI GKGCMC 1 cut(s) 1660
BanI GGYRCC 1 cut(s) 1655
BarI GAAGNNNNNNTAC 2 cut(s) 1552, 1584
Bbv12I GWGCWC 1 cut(s) 934
BbvI GCAGC 3 cut(s) 36, 1607, 1610
BccI CCATC 5 cut(s) 669, 844, 1008, 1451, 1532
BceAI ACGGC 1 cut(s) 1345
BciVI GTATCC 2 cut(s) 334, 799
BcoDI GTCTC 6 cut(s) 215, 272, 409, 834, 1368, 1638
BcuI ACTAGT 1 cut(s) 1556
BfaI CTAG 9 cut(s) 218, 665, 672, 740, 986, 1274, 1412, 1557, 1688
BfuI GTATCC 2 cut(s) 334, 799
BglII AGATCT 1 cut(s) 775
BisI GCNGC 3 cut(s) 25, 1596, 1599
BlpI GCTNAGC 1 cut(s) 16
BlsI GCNGC 3 cut(s) 26, 1597, 1600
BmgBI CACGTC 1 cut(s) 1454
BmgT120I GGNCC 1 cut(s) 541
BmiI GGNNCC 2 cut(s) 380, 1657
BmsI GCATC 3 cut(s) 196, 627, 697
BplI GAGNNNNNCTC 2 cut(s) 513, 545
Bpu1102I GCTNAGC 1 cut(s) 16
Bpu14I TTCGAA 1 cut(s) 1400
BpuEI CTTGAG 1 cut(s) 579
Bsa29I ATCGAT 1 cut(s) 271
BsaAI YACGTR 1 cut(s) 1144
BsaHI GRCGYC 1 cut(s) 1198
BsaI GGTCTC 1 cut(s) 272
BsaJI CCNNGG 1 cut(s) 508
BsaXI ACNNNNNCTCC 2 cut(s) 1178, 1208
Bsc4I CCNNNNNNNGG 1 cut(s) 1245
Bse1I ACTGG 2 cut(s) 43, 282
Bse3DI GCAATG 1 cut(s) 641
BseCI ATCGAT 1 cut(s) 271
BseDI CCNNGG 1 cut(s) 508
BseGI GGATG 4 cut(s) 541, 985, 1024, 1131
BseLI CCNNNNNNNGG 1 cut(s) 1245
BseMI GCAATG 1 cut(s) 641
BseMII CTCAG 2 cut(s) 382, 910
BseNI ACTGG 2 cut(s) 43, 282
BseRI GAGGAG 1 cut(s) 197
BseSI GKGCMC 1 cut(s) 1660
BseXI GCAGC 3 cut(s) 36, 1607, 1610
BseYI CCCAGC 1 cut(s) 575
BsgI GTGCAG 1 cut(s) 338
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 2 cut(s) 542, 1360
BshNI GGYRCC 1 cut(s) 1655
BshVI ATCGAT 1 cut(s) 271
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 934
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 1 cut(s) 1245
BsmAI GTCTC 6 cut(s) 215, 272, 409, 834, 1368, 1638
BsmBI CGTCTC 2 cut(s) 1368, 1638
BsmFI GGGAC 1 cut(s) 279
BsmI GAATGC 3 cut(s) 548, 660, 1030
BsnI GGCC 2 cut(s) 542, 1360
Bso31I GGTCTC 1 cut(s) 272
Bsp119I TTCGAA 1 cut(s) 1400
Bsp1286I GDGCHC 2 cut(s) 934, 1660
Bsp1407I TGTACA 1 cut(s) 1699
Bsp143I GATC 6 cut(s) 133, 177, 187, 775, 1345, 1683
Bsp1720I GCTNAGC 1 cut(s) 16
BspACI CCGC 1 cut(s) 27
BspANI GGCC 2 cut(s) 542, 1360
BspCNI CTCAG 2 cut(s) 381, 909
BspDI ATCGAT 1 cut(s) 271
BspHI TCATGA 1 cut(s) 142
BspLI GGNNCC 2 cut(s) 380, 1657
BspPI GGATC 2 cut(s) 195, 1678
BspT104I TTCGAA 1 cut(s) 1400
BspT107I GGYRCC 1 cut(s) 1655
BspTNI GGTCTC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 641
BsrGI TGTACA 1 cut(s) 1699
BsrI ACTGG 2 cut(s) 43, 282
BssECI CCNNGG 1 cut(s) 508
BssMI GATC 6 cut(s) 133, 177, 187, 775, 1345, 1683
BssNI GRCGYC 1 cut(s) 1198
BssT1I CCWWGG 1 cut(s) 508
Bst4CI ACNGT 2 cut(s) 695, 1087
BstACI GRCGYC 1 cut(s) 1198
BstAUI TGTACA 1 cut(s) 1699
BstBAI YACGTR 1 cut(s) 1144
BstBI TTCGAA 1 cut(s) 1400
BstC8I GCNNGC 3 cut(s) 22, 560, 1486
BstDEI CTNAG 7 cut(s) 16, 368, 383, 896, 905, 1309, 1330
BstF5I GGATG 4 cut(s) 541, 985, 1024, 1131
BstKTI GATC 6 cut(s) 136, 180, 190, 778, 1348, 1686
BstMAI GTCTC 6 cut(s) 215, 272, 409, 834, 1368, 1638
BstMBI GATC 6 cut(s) 133, 177, 187, 775, 1345, 1683
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 4 cut(s) 21, 30, 444, 1619
BstNSI RCATGY 2 cut(s) 562, 590
BstSLI GKGCMC 1 cut(s) 1660
BstV1I GCAGC 3 cut(s) 36, 1607, 1610
BstX2I RGATCY 3 cut(s) 187, 775, 1683
BstXI CCANNNNNNTGG 1 cut(s) 96
BstYI RGATCY 3 cut(s) 187, 775, 1683
Bsu15I ATCGAT 1 cut(s) 271
BsuI GTATCC 2 cut(s) 334, 799
BsuRI GGCC 2 cut(s) 542, 1360
BsuTUI ATCGAT 1 cut(s) 271
BtrI CACGTC 1 cut(s) 1454
BtsCI GGATG 4 cut(s) 541, 985, 1024, 1131
BtsI GCAGTG 2 cut(s) 360, 1698
BtsIMutI CAGTG 5 cut(s) 360, 934, 1083, 1260, 1698
Cac8I GCNNGC 3 cut(s) 22, 560, 1486
CaiI CAGNNNCTG 1 cut(s) 1259
CciI TCATGA 1 cut(s) 142
Cfr13I GGNCC 1 cut(s) 541
ClaI ATCGAT 1 cut(s) 271
CseI GACGC 1 cut(s) 1187
Csp6I GTAC 3 cut(s) 1210, 1554, 1700
CviAII CATG 7 cut(s) 143, 392, 559, 587, 626, 1245, 1343
CviQI GTAC 3 cut(s) 1210, 1554, 1700
DdeI CTNAG 7 cut(s) 16, 368, 383, 896, 905, 1309, 1330
DpnI GATC 6 cut(s) 135, 179, 189, 777, 1347, 1685
DpnII GATC 6 cut(s) 133, 177, 187, 775, 1345, 1683
EaeI YGGCCR 1 cut(s) 1358
Eco130I CCWWGG 1 cut(s) 508
Eco31I GGTCTC 1 cut(s) 272
Eco32I GATATC 2 cut(s) 247, 1251
Eco57I CTGAAG 2 cut(s) 98, 1076
EcoO109I RGGNCCY 1 cut(s) 541
EcoRV GATATC 2 cut(s) 247, 1251
EcoT14I CCWWGG 1 cut(s) 508
EcoT22I ATGCAT 3 cut(s) 631, 1246, 1631
ErhI CCWWGG 1 cut(s) 508
Esp3I CGTCTC 2 cut(s) 1368, 1638
FaeI CATG 7 cut(s) 146, 395, 562, 590, 629, 1248, 1346
FalI AAGNNNNNCTT 2 cut(s) 1218, 1250
FaqI GGGAC 1 cut(s) 279
FatI CATG 7 cut(s) 142, 391, 558, 586, 625, 1244, 1342
FblI GTMKAC 2 cut(s) 5, 153
Fnu4HI GCNGC 3 cut(s) 25, 1596, 1599
FokI GGATG 4 cut(s) 548, 992, 1031, 1118
Fsp4HI GCNGC 3 cut(s) 25, 1596, 1599
FspBI CTAG 9 cut(s) 218, 665, 672, 740, 986, 1274, 1412, 1557, 1688
GluI GCNGC 3 cut(s) 25, 1596, 1599
GsaI CCCAGC 1 cut(s) 579
HaeIII GGCC 2 cut(s) 542, 1360
HgaI GACGC 1 cut(s) 1187
Hin1I GRCGYC 1 cut(s) 1198
Hin1II CATG 7 cut(s) 146, 395, 562, 590, 629, 1248, 1346
HincII GTYRAC 1 cut(s) 1182
HindII GTYRAC 1 cut(s) 1182
HindIII AAGCTT 2 cut(s) 498, 1152
HinfI GANTC 9 cut(s) 100, 212, 273, 316, 487, 622, 1036, 1336, 1478
HpaI GTTAAC 1 cut(s) 1182
HphI GGTGA 3 cut(s) 544, 1331, 1433
Hpy166II GTNNAC 5 cut(s) 6, 154, 1129, 1182, 1212
Hpy188I TCNGA 4 cut(s) 57, 192, 1045, 1471
Hpy188III TCNNGA 4 cut(s) 104, 143, 277, 320
Hpy8I GTNNAC 5 cut(s) 6, 154, 1129, 1182, 1212
Hpy99I CGWCG 1 cut(s) 443
HpyAV CCTTC 5 cut(s) 512, 796, 1108, 1145, 1396
HpyCH4III ACNGT 2 cut(s) 695, 1087
HpyCH4IV ACGT 2 cut(s) 1143, 1453
HpyF10VI GCNNNNNNNGC 4 cut(s) 21, 30, 444, 1619
HpyF3I CTNAG 7 cut(s) 16, 368, 383, 896, 905, 1309, 1330
HpySE526I ACGT 2 cut(s) 1143, 1453
Hsp92I GRCGYC 1 cut(s) 1198
Hsp92II CATG 7 cut(s) 146, 395, 562, 590, 629, 1248, 1346
KspAI GTTAAC 1 cut(s) 1182
Kzo9I GATC 6 cut(s) 133, 177, 187, 775, 1345, 1683
LmnI GCTCC 2 cut(s) 384, 1117
LpnPI CCDG 8 cut(s) 24, 295, 568, 589, 1245, 1456, 1674, 1694
Lsp1109I GCAGC 3 cut(s) 36, 1607, 1610
LweI GCATC 3 cut(s) 196, 627, 697
MaeI CTAG 9 cut(s) 218, 665, 672, 740, 986, 1274, 1412, 1557, 1688
MaeII ACGT 2 cut(s) 1143, 1453
MaeIII GTNAC 5 cut(s) 101, 213, 589, 1199, 1393
MalI GATC 6 cut(s) 135, 179, 189, 777, 1347, 1685
MboI GATC 6 cut(s) 133, 177, 187, 775, 1345, 1683
MboII GAAGA 7 cut(s) 104, 158, 325, 1238, 1534, 1537, 1560
MfeI CAATTG 1 cut(s) 945
MflI RGATCY 3 cut(s) 187, 775, 1683
MhlI GDGCHC 2 cut(s) 934, 1660
MlyI GAGTC 4 cut(s) 109, 221, 496, 631
MmeI TCCRAC 2 cut(s) 274, 1499
Mph1103I ATGCAT 3 cut(s) 631, 1246, 1631
MseI TTAA 6 cut(s) 63, 780, 1181, 1296, 1323, 1711
MslI CAYNNNNRTG 3 cut(s) 57, 557, 1324
MspA1I CMGCKG 1 cut(s) 27
MunI CAATTG 1 cut(s) 945
Mva1269I GAATGC 3 cut(s) 548, 660, 1030
MwoI GCNNNNNNNGC 4 cut(s) 21, 30, 444, 1619
NdeII GATC 6 cut(s) 133, 177, 187, 775, 1345, 1683
NlaIII CATG 7 cut(s) 146, 395, 562, 590, 629, 1248, 1346
NlaIV GGNNCC 2 cut(s) 380, 1657
NmuCI GTSAC 3 cut(s) 101, 213, 1199
NsiI ATGCAT 3 cut(s) 631, 1246, 1631
NspI RCATGY 2 cut(s) 562, 590
NspV TTCGAA 1 cut(s) 1400
PaeI GCATGC 1 cut(s) 562
PagI TCATGA 1 cut(s) 142
PctI GAATGC 3 cut(s) 548, 660, 1030
PfeI GAWTC 5 cut(s) 273, 316, 1036, 1336, 1478
PflMI CCANNNNNTGG 1 cut(s) 1245
PkrI GCNGC 3 cut(s) 26, 1597, 1600
Ple19I CGATCG 1 cut(s) 180
PleI GAGTC 4 cut(s) 108, 220, 495, 630
PpsI GAGTC 4 cut(s) 108, 220, 495, 630
Ppu21I YACGTR 1 cut(s) 1144
PspFI CCCAGC 1 cut(s) 575
PspN4I GGNNCC 2 cut(s) 380, 1657
PspPI GGNCC 1 cut(s) 541
PstNI CAGNNNCTG 1 cut(s) 1259
PsuI RGATCY 3 cut(s) 187, 775, 1683
PvuI CGATCG 1 cut(s) 180
RsaI GTAC 3 cut(s) 1211, 1555, 1701
RsaNI GTAC 3 cut(s) 1210, 1554, 1700
RseI CAYNNNNRTG 3 cut(s) 57, 557, 1324
SaqAI TTAA 6 cut(s) 63, 780, 1181, 1296, 1323, 1711
SatI GCNGC 3 cut(s) 25, 1596, 1599
Sau3AI GATC 6 cut(s) 133, 177, 187, 775, 1345, 1683
Sau96I GGNCC 1 cut(s) 541
SchI GAGTC 4 cut(s) 109, 221, 496, 631
SduI GDGCHC 2 cut(s) 934, 1660
SfaNI GCATC 3 cut(s) 196, 627, 697
SfuI TTCGAA 1 cut(s) 1400
SmiMI CAYNNNNRTG 3 cut(s) 57, 557, 1324
SmlI CTYRAG 1 cut(s) 594
SmoI CTYRAG 1 cut(s) 594
SpeI ACTAGT 1 cut(s) 1556
SphI GCATGC 1 cut(s) 562
SsiI CCGC 1 cut(s) 27
SspMI CTAG 9 cut(s) 218, 665, 672, 740, 986, 1274, 1412, 1557, 1688
StyI CCWWGG 1 cut(s) 508
TaaI ACNGT 2 cut(s) 695, 1087
TaiI ACGT 2 cut(s) 1146, 1456
TaqI TCGA 6 cut(s) 176, 180, 271, 276, 1400, 1615
TatI WGTACW 2 cut(s) 1209, 1699
TfiI GAWTC 5 cut(s) 273, 316, 1036, 1336, 1478
Tru1I TTAA 6 cut(s) 63, 780, 1181, 1296, 1323, 1711
Tru9I TTAA 6 cut(s) 63, 780, 1181, 1296, 1323, 1711
TscAI CASTG 5 cut(s) 367, 934, 1090, 1260, 1698
TseFI GTSAC 3 cut(s) 101, 213, 1199
TseI GCWGC 3 cut(s) 24, 1595, 1598
Tsp45I GTSAC 3 cut(s) 101, 213, 1199
TspDTI ATGAA 7 cut(s) 159, 492, 996, 1107, 1119, 1329, 1544
TspGWI ACGGA 2 cut(s) 446, 1199
TspRI CASTG 5 cut(s) 367, 934, 1090, 1260, 1698
Van91I CCANNNNNTGG 1 cut(s) 1245
XapI RAATTY 3 cut(s) 423, 728, 1586
XceI RCATGY 2 cut(s) 562, 590
XmiI GTMKAC 2 cut(s) 5, 153
XspI CTAG 9 cut(s) 218, 665, 672, 740, 986, 1274, 1412, 1557, 1688
Zsp2I ATGCAT 3 cut(s) 631, 1246, 1631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.