pycom12g00970

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012658
Physical Location & Seq
Reverse (-)
827558 .. 828330
773 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g00970.3

Sequence Viewer

Length: 267 bp
ATGGCGCACAAGTGTAATTTTATGACACAACTAGTTCTCTTTCTATGTTTCACGTATGTCTTCTCGTCAATTGCTATTGCTCCTACGACAAGAATCGATCATATAGGAAGCCAGAAGATAAATAAGGAAGACCTACTGAGTCAGAATTTGCTGGATGAAGGTGTCCTGGATTTGGGAGAAGGATTTGTGGAGGGGCGGATGGAGCTGGAAACCAATGATTATTCAGGGGTAGGAGCAAATCCGGATCATGATCCAAAACCCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

89

Amino Acids

9.75

Weight (kDa)

4.6

Isoelectric Point (pI)

23.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 241
AciI CCGC 1 cut(s) 196
AclWI GGATC 2 cut(s) 245, 252
AcsI RAATTY 1 cut(s) 145
AfiI CCNNNNNNNGG 1 cut(s) 172
AhlI ACTAGT 1 cut(s) 31
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 1 cut(s) 205
AluI AGCT 1 cut(s) 205
AlwI GGATC 2 cut(s) 245, 252
Aor13HI TCCGGA 1 cut(s) 241
ApoI RAATTY 1 cut(s) 145
AspLEI GCGC 1 cut(s) 7
BbsI GAAGAC 2 cut(s) 52, 135
BccI CCATC 1 cut(s) 193
BciT130I CCWGG 1 cut(s) 167
BcuI ACTAGT 1 cut(s) 31
BfaI CTAG 1 cut(s) 32
Bme1390I CCNGG 1 cut(s) 167
BmrFI CCNGG 1 cut(s) 167
BpiI GAAGAC 2 cut(s) 52, 135
Bsa29I ATCGAT 1 cut(s) 96
BsaAI YACGTR 1 cut(s) 54
BsaBI GATNNNNATC 1 cut(s) 249
BsaWI WCCGGW 1 cut(s) 241
Bsc4I CCNNNNNNNGG 1 cut(s) 172
Bse8I GATNNNNATC 1 cut(s) 249
BseAI TCCGGA 1 cut(s) 241
BseBI CCWGG 1 cut(s) 167
BseCI ATCGAT 1 cut(s) 96
BseGI GGATG 2 cut(s) 160, 204
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 1 cut(s) 172
BseMII CTCAG 1 cut(s) 128
BshVI ATCGAT 1 cut(s) 96
BsiSI CCGG 1 cut(s) 242
BslI CCNNNNNNNGG 1 cut(s) 172
Bsp13I TCCGGA 1 cut(s) 241
Bsp143I GATC 3 cut(s) 97, 244, 250
BspACI CCGC 1 cut(s) 196
BspCNI CTCAG 1 cut(s) 129
BspDI ATCGAT 1 cut(s) 96
BspEI TCCGGA 1 cut(s) 241
BspHI TCATGA 1 cut(s) 247
BspPI GGATC 2 cut(s) 245, 252
BssMI GATC 3 cut(s) 97, 244, 250
Bst2UI CCWGG 1 cut(s) 167
BstBAI YACGTR 1 cut(s) 54
BstDEI CTNAG 1 cut(s) 137
BstF5I GGATG 2 cut(s) 160, 204
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 3 cut(s) 100, 247, 253
BstMBI GATC 3 cut(s) 97, 244, 250
BstMWI GCNNNNNNNGC 1 cut(s) 202
BstNI CCWGG 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 165
BstV2I GAAGAC 2 cut(s) 52, 135
Bsu15I ATCGAT 1 cut(s) 96
BsuTUI ATCGAT 1 cut(s) 96
BtsCI GGATG 2 cut(s) 160, 204
CciI TCATGA 1 cut(s) 247
CfoI GCGC 1 cut(s) 7
ClaI ATCGAT 1 cut(s) 96
CviAII CATG 2 cut(s) 248, 264
CviJI RGCY 2 cut(s) 111, 205
CviKI_1 RGCY 2 cut(s) 111, 205
DdeI CTNAG 1 cut(s) 137
DpnI GATC 3 cut(s) 99, 246, 252
DpnII GATC 3 cut(s) 97, 244, 250
EciI GGCGGA 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 165
FaeI CATG 2 cut(s) 251, 267
FaiI YATR 7 cut(s) 23, 46, 57, 102, 104, 249, 265
FatI CATG 2 cut(s) 247, 263
FokI GGATG 2 cut(s) 167, 211
FspBI CTAG 1 cut(s) 32
GlaI GCGC 1 cut(s) 6
HapII CCGG 1 cut(s) 242
HhaI GCGC 1 cut(s) 7
Hin1II CATG 2 cut(s) 251, 267
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 2 cut(s) 93, 139
HpaII CCGG 1 cut(s) 242
Hpy188I TCNGA 1 cut(s) 144
Hpy188III TCNNGA 2 cut(s) 242, 248
HpyAV CCTTC 2 cut(s) 152, 173
HpyCH4IV ACGT 1 cut(s) 53
HpyF10VI GCNNNNNNNGC 1 cut(s) 202
HpyF3I CTNAG 1 cut(s) 137
HpySE526I ACGT 1 cut(s) 53
Hsp92II CATG 2 cut(s) 251, 267
HspAI GCGC 1 cut(s) 5
Kpn2I TCCGGA 1 cut(s) 241
Kzo9I GATC 3 cut(s) 97, 244, 250
LmnI GCTCC 3 cut(s) 85, 202, 233
LpnPI CCDG 7 cut(s) 125, 137, 152, 179, 191, 210, 255
MaeI CTAG 1 cut(s) 32
MaeII ACGT 1 cut(s) 53
MalI GATC 3 cut(s) 99, 246, 252
MboI GATC 3 cut(s) 97, 244, 250
MboII GAAGA 3 cut(s) 52, 127, 140
MfeI CAATTG 1 cut(s) 69
MluCI AATT 3 cut(s) 16, 69, 145
MlyI GAGTC 1 cut(s) 148
MnlI CCTC 1 cut(s) 184
MroI TCCGGA 1 cut(s) 241
MspI CCGG 1 cut(s) 242
MspR9I CCNGG 1 cut(s) 167
MunI CAATTG 1 cut(s) 69
MvaI CCWGG 1 cut(s) 167
MwoI GCNNNNNNNGC 1 cut(s) 202
NdeII GATC 3 cut(s) 97, 244, 250
NlaIII CATG 2 cut(s) 251, 267
PagI TCATGA 1 cut(s) 247
PfeI GAWTC 1 cut(s) 93
PfoI TCCNGGA 1 cut(s) 165
PleI GAGTC 1 cut(s) 147
PpsI GAGTC 1 cut(s) 147
Ppu21I YACGTR 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 165
PspGI CCWGG 1 cut(s) 165
Sau3AI GATC 3 cut(s) 97, 244, 250
SchI GAGTC 1 cut(s) 148
ScrFI CCNGG 1 cut(s) 167
SetI ASST 4 cut(s) 56, 135, 163, 207
SpeI ACTAGT 1 cut(s) 31
Sse9I AATT 3 cut(s) 16, 69, 145
SsiI CCGC 1 cut(s) 196
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 165
TaiI ACGT 1 cut(s) 56
TaqI TCGA 1 cut(s) 96
TasI AATT 3 cut(s) 16, 69, 145
TfiI GAWTC 1 cut(s) 93
TspDTI ATGAA 1 cut(s) 171
XapI RAATTY 1 cut(s) 145
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.