MD14G1031500.v1.1

Mitochondrial fission protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003516
Physical Location & Seq
Forward (+)
2810409 .. 2813810
3402 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1031500.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGGCGGAGATGATCCAAGGTGGGGTCCACCGAGCAACCGGAATCAGAAGAGCCGTCGTGATCGGAAACGGGTTCGCCGGCGCGGAGAATCAGAGCATTGGTTTGGTTCGTGCGCTCGGCCTATGGGGTCGTCAGTCCCTTTACCGTGTTAGGCGGCCAAGAGGAGGGATTAACGAGTGGCTTCACTGGCTTCCACTTCCTCTCCATAAAAAAGTGGTGCCTTTGCATCTTTCTTCTGAAAAATTAGGCCTCTCATCTGATATTCCGGAAGCTGATGCAAAGCAGATTGCAGAAATGGCCCGTCGAACATTCGACAAAGATGGCCCGTTGCTGGTGGTTGCATCTGGTCGGGACACCATTCCTGTTTCAAGCGCCATAAAACAGTTAGCTCCCGAAAATGTTTTTCTTGTCCAGATTCAACATCCAAGGTCGCATGTGGATAGGTTTGATTTGGTGATAGCTCCTCGTCATGATTATTACCCATTGACTCCCCATGCACAGAAGCAAATTCCTTGGTTTCTTCGGAGGTGGATCACTCCACGTGAACCACCAGGCAAAAATGTTTTTCTCACTGTGGGAGCTCTTCATCAGGCTGATTTTGCTGCACTTAAGAGTGCTGCTTCTGTCTGGCATGCCGAATTGACACCACTTCCAAAACCTTTGCTTGTGGTTAATATCGGAGGCCCTTCAAGAAATTGTTCATATGATGCGGATCTTGCGAAACATTTAGCAGCTATGCTGCAGAATGTTCTTTGGAGCTGTGGAAGTGTGAGGATATCTTTCTCTAGGAGAACTCCCGAACAGGTGTCCAGAGTTTTGAGGAAGGAATTCAGTACTAATCCCAAAGTCTACATTTGGGATGGTGAAGGTCGCAATCCACATATGGGCCATCTGGCCTGTGCCGATGCTTTTGTCATTACCGCTGATTCAGTTAGTATGTTGAGTGAGGCTTGTAGTACGGGCAAACCTGTTTATGTGATTGGTGCGGAACACTGTACATGGAAGTTTGCAGACTTCCAGAACTCTCTGCGGGAACGAGGAGCGGTTCGACCATTAACCGGTAAAGAAGATATGTCCCTGCGTTGGAGCTATACACCACTGGATGACACTGCCGAGGCTGCTTGGCGGGTGAACAAGGCACTCGCTGAGCGTGGATGGAGTATACAACTTACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mito_fiss_Elm1

Protein Analysis

392

Amino Acids

43.52

Weight (kDa)

9.6

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_fiss_Elm1 PF06258 26 - 380 3.6e-110 Mitochondrial fission ELM1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 217
AccBSI CCGCTC 1 cut(s) 1043
AccI GTMKAC 2 cut(s) 849, 1162
AccII CGCG 1 cut(s) 83
AccIII TCCGGA 1 cut(s) 265
AciI CCGC 9 cut(s) 5, 83, 154, 710, 921, 986, 1030, 1043, 1126
AclWI GGATC 3 cut(s) 7, 539, 720
AcoI YGGCCR 1 cut(s) 155
AcsI RAATTY 2 cut(s) 507, 827
AcvI CACGTG 1 cut(s) 542
AfaI GTAC 3 cut(s) 835, 958, 997
AfiI CCNNNNNNNGG 6 cut(s) 22, 164, 331, 884, 1058, 1083
AflII CTTAAG 1 cut(s) 608
AgeI ACCGGT 1 cut(s) 1058
AgsI TTSAA 3 cut(s) 369, 419, 690
AjnI CCWGG 1 cut(s) 550
AluBI AGCT 7 cut(s) 272, 389, 461, 581, 734, 759, 1089
AluI AGCT 7 cut(s) 272, 389, 461, 581, 734, 759, 1089
Alw21I GWGCWC 1 cut(s) 583
AlwI GGATC 3 cut(s) 7, 539, 720
Aor13HI TCCGGA 1 cut(s) 265
AoxI GGCC 8 cut(s) 118, 155, 247, 297, 322, 682, 886, 894
ApeKI GCWGC 5 cut(s) 602, 617, 731, 739, 1118
ApoI RAATTY 2 cut(s) 507, 827
AsiGI ACCGGT 1 cut(s) 1058
Asp700I GAANNNNTTC 2 cut(s) 697, 827
AspLEI GCGC 3 cut(s) 83, 115, 374
AspS9I GGNCC 5 cut(s) 25, 298, 323, 683, 886
AsuHPI GGTGA 3 cut(s) 466, 875, 1141
AvaII GGWCC 1 cut(s) 25
BanI GGYRCC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 583
BbrPI CACGTG 1 cut(s) 542
Bbv12I GWGCWC 1 cut(s) 583
BbvI GCAGC 5 cut(s) 589, 604, 726, 743, 1105
BccI CCATC 4 cut(s) 314, 854, 897, 1149
BceAI ACGGC 1 cut(s) 38
BciT130I CCWGG 1 cut(s) 552
BfaI CTAG 1 cut(s) 786
BfmI CTRYAG 1 cut(s) 740
BfoI RGCGCY 1 cut(s) 375
BfrI CTTAAG 1 cut(s) 608
BisI GCNGC 6 cut(s) 155, 603, 618, 732, 740, 1119
BlpI GCTNAGC 1 cut(s) 1146
BlsI GCNGC 6 cut(s) 156, 604, 619, 733, 741, 1120
BmcAI AGTACT 1 cut(s) 835
Bme1390I CCNGG 1 cut(s) 552
Bme18I GGWCC 1 cut(s) 25
BmgT120I GGNCC 5 cut(s) 25, 298, 323, 683, 886
BmiI GGNNCC 2 cut(s) 26, 219
BmrFI CCNGG 1 cut(s) 552
BmsI GCATC 5 cut(s) 235, 265, 350, 697, 895
Bpu1102I GCTNAGC 1 cut(s) 1146
BsaAI YACGTR 1 cut(s) 542
BsaBI GATNNNNATC 1 cut(s) 711
BsaJI CCNNGG 4 cut(s) 16, 425, 512, 1113
BsaWI WCCGGW 3 cut(s) 38, 265, 1058
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 6 cut(s) 22, 164, 331, 884, 1058, 1083
Bse118I RCCGGY 2 cut(s) 77, 1058
Bse1I ACTGG 2 cut(s) 191, 1104
Bse8I GATNNNNATC 1 cut(s) 711
BseAI TCCGGA 1 cut(s) 265
BseBI CCWGG 1 cut(s) 552
BseDI CCNNGG 4 cut(s) 16, 425, 512, 1113
BseGI GGATG 4 cut(s) 421, 865, 1108, 1160
BseJI GATNNNNATC 1 cut(s) 711
BseLI CCNNNNNNNGG 6 cut(s) 22, 164, 331, 884, 1058, 1083
BseMII CTCAG 1 cut(s) 1137
BseNI ACTGG 2 cut(s) 191, 1104
BseRI GAGGAG 3 cut(s) 177, 453, 1053
BseXI GCAGC 5 cut(s) 589, 604, 726, 743, 1105
BsgI GTGCAG 1 cut(s) 588
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 8 cut(s) 120, 157, 249, 299, 324, 684, 888, 896
BshNI GGYRCC 1 cut(s) 217
BshTI ACCGGT 1 cut(s) 1058
BsiHKAI GWGCWC 1 cut(s) 583
BsiSI CCGG 4 cut(s) 39, 78, 266, 1059
BslFI GGGAC 3 cut(s) 121, 365, 1060
BslI CCNNNNNNNGG 6 cut(s) 22, 164, 331, 884, 1058, 1083
BsmFI GGGAC 3 cut(s) 121, 365, 1060
BsnI GGCC 8 cut(s) 120, 157, 249, 299, 324, 684, 888, 896
Bsp1286I GDGCHC 1 cut(s) 583
Bsp13I TCCGGA 1 cut(s) 265
Bsp1407I TGTACA 1 cut(s) 995
Bsp143I GATC 4 cut(s) 12, 60, 531, 712
Bsp1720I GCTNAGC 1 cut(s) 1146
BspACI CCGC 9 cut(s) 5, 83, 154, 710, 921, 986, 1030, 1043, 1126
BspANI GGCC 8 cut(s) 120, 157, 249, 299, 324, 684, 888, 896
BspCNI CTCAG 1 cut(s) 1138
BspEI TCCGGA 1 cut(s) 265
BspFNI CGCG 1 cut(s) 83
BspHI TCATGA 1 cut(s) 469
BspLI GGNNCC 2 cut(s) 26, 219
BspMAI CTGCAG 1 cut(s) 744
BspPI GGATC 3 cut(s) 7, 539, 720
BspQI GCTCTTC 2 cut(s) 43, 588
BspT107I GGYRCC 1 cut(s) 217
BspTI CTTAAG 1 cut(s) 608
BsrBI CCGCTC 1 cut(s) 1043
BsrFI RCCGGY 2 cut(s) 77, 1058
BsrGI TGTACA 1 cut(s) 995
BsrI ACTGG 2 cut(s) 191, 1104
BssAI RCCGGY 2 cut(s) 77, 1058
BssECI CCNNGG 4 cut(s) 16, 425, 512, 1113
BssMI GATC 4 cut(s) 12, 60, 531, 712
BssNAI GTATAC 1 cut(s) 1163
BssT1I CCWWGG 3 cut(s) 16, 425, 512
Bst1107I GTATAC 1 cut(s) 1163
Bst2UI CCWGG 1 cut(s) 552
Bst4CI ACNGT 4 cut(s) 146, 384, 574, 995
Bst6I CTCTTC 2 cut(s) 43, 588
BstAFI CTTAAG 1 cut(s) 608
BstAUI TGTACA 1 cut(s) 995
BstBAI YACGTR 1 cut(s) 542
BstC8I GCNNGC 2 cut(s) 79, 633
BstDEI CTNAG 1 cut(s) 1146
BstF5I GGATG 4 cut(s) 421, 865, 1108, 1160
BstFNI CGCG 1 cut(s) 83
BstH2I RGCGCY 1 cut(s) 375
BstHHI GCGC 3 cut(s) 83, 115, 374
BstKTI GATC 4 cut(s) 15, 63, 534, 715
BstMBI GATC 4 cut(s) 12, 60, 531, 712
BstMWI GCNNNNNNNGC 5 cut(s) 187, 296, 599, 716, 1118
BstNI CCWGG 1 cut(s) 552
BstNSI RCATGY 2 cut(s) 437, 635
BstSCI CCNGG 1 cut(s) 550
BstSFI CTRYAG 1 cut(s) 740
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 5 cut(s) 589, 604, 726, 743, 1105
BstX2I RGATCY 1 cut(s) 712
BstYI RGATCY 1 cut(s) 712
BstZ17I GTATAC 1 cut(s) 1163
BsuRI GGCC 8 cut(s) 120, 157, 249, 299, 324, 684, 888, 896
BtsCI GGATG 4 cut(s) 421, 865, 1108, 1160
BtsI GCAGTG 1 cut(s) 1107
BtsIMutI CAGTG 5 cut(s) 184, 570, 991, 1097, 1107
Cac8I GCNNGC 2 cut(s) 79, 633
CciI TCATGA 1 cut(s) 469
CfoI GCGC 3 cut(s) 83, 115, 374
Cfr10I RCCGGY 2 cut(s) 77, 1058
Cfr13I GGNCC 5 cut(s) 25, 298, 323, 683, 886
Csp6I GTAC 3 cut(s) 834, 957, 996
CspAI ACCGGT 1 cut(s) 1058
CviAII CATG 5 cut(s) 434, 470, 494, 632, 999
CviQI GTAC 3 cut(s) 834, 957, 996
DdeI CTNAG 1 cut(s) 1146
DpnI GATC 4 cut(s) 14, 62, 533, 714
DpnII GATC 4 cut(s) 12, 60, 531, 712
EaeI YGGCCR 1 cut(s) 155
Eam1104I CTCTTC 2 cut(s) 43, 588
EarI CTCTTC 2 cut(s) 43, 588
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 581
Eco130I CCWWGG 3 cut(s) 16, 425, 512
Eco147I AGGCCT 1 cut(s) 249
Eco24I GRGCYC 1 cut(s) 583
Eco32I GATATC 1 cut(s) 777
Eco47I GGWCC 1 cut(s) 25
Eco53kI GAGCTC 1 cut(s) 581
Eco72I CACGTG 1 cut(s) 542
EcoICRI GAGCTC 1 cut(s) 581
EcoO109I RGGNCCY 1 cut(s) 683
EcoRI GAATTC 1 cut(s) 827
EcoRII CCWGG 1 cut(s) 550
EcoRV GATATC 1 cut(s) 777
EcoT14I CCWWGG 3 cut(s) 16, 425, 512
EcoT38I GRGCYC 1 cut(s) 583
ErhI CCWWGG 3 cut(s) 16, 425, 512
FaeI CATG 5 cut(s) 437, 473, 497, 635, 1002
FaqI GGGAC 3 cut(s) 121, 365, 1060
FatI CATG 5 cut(s) 433, 469, 493, 631, 998
FauI CCCGC 2 cut(s) 1023, 1119
FauNDI CATATG 2 cut(s) 703, 882
FblI GTMKAC 2 cut(s) 849, 1162
Fnu4HI GCNGC 6 cut(s) 155, 603, 618, 732, 740, 1119
FokI GGATG 4 cut(s) 408, 872, 1115, 1167
FriOI GRGCYC 1 cut(s) 583
Fsp4HI GCNGC 6 cut(s) 155, 603, 618, 732, 740, 1119
FspBI CTAG 1 cut(s) 786
GlaI GCGC 3 cut(s) 82, 114, 373
GluI GCNGC 6 cut(s) 155, 603, 618, 732, 740, 1119
HaeII RGCGCY 1 cut(s) 375
HaeIII GGCC 8 cut(s) 120, 157, 249, 299, 324, 684, 888, 896
HapII CCGG 4 cut(s) 39, 78, 266, 1059
HhaI GCGC 3 cut(s) 83, 115, 374
Hin1II CATG 5 cut(s) 437, 473, 497, 635, 1002
Hin6I GCGC 3 cut(s) 81, 113, 372
HinP1I GCGC 3 cut(s) 81, 113, 372
HinfI GANTC 5 cut(s) 42, 88, 415, 487, 926
HpaII CCGG 4 cut(s) 39, 78, 266, 1059
HphI GGTGA 3 cut(s) 466, 875, 1141
Hpy166II GTNNAC 5 cut(s) 28, 545, 850, 1132, 1163
Hpy188I TCNGA 7 cut(s) 47, 65, 93, 238, 259, 525, 680
Hpy8I GTNNAC 5 cut(s) 28, 545, 850, 1132, 1163
Hpy99I CGWCG 2 cut(s) 59, 306
HpyAV CCTTC 3 cut(s) 696, 817, 860
HpyCH4III ACNGT 4 cut(s) 146, 384, 574, 995
HpyCH4IV ACGT 1 cut(s) 541
HpyCH4V TGCA 8 cut(s) 226, 278, 290, 341, 497, 605, 742, 1010
HpyF10VI GCNNNNNNNGC 5 cut(s) 187, 296, 599, 716, 1118
HpyF3I CTNAG 1 cut(s) 1146
HpySE526I ACGT 1 cut(s) 541
Hsp92II CATG 5 cut(s) 437, 473, 497, 635, 1002
HspAI GCGC 3 cut(s) 81, 113, 372
Kpn2I TCCGGA 1 cut(s) 265
KroI GCCGGC 1 cut(s) 77
KroNI GCCGGC 1 cut(s) 79
Kzo9I GATC 4 cut(s) 12, 60, 531, 712
LguI GCTCTTC 2 cut(s) 43, 588
LmnI GCTCC 6 cut(s) 394, 466, 578, 756, 1040, 1086
Lsp1109I GCAGC 5 cut(s) 589, 604, 726, 743, 1105
LweI GCATC 5 cut(s) 235, 265, 350, 697, 895
MaeI CTAG 1 cut(s) 786
MaeII ACGT 1 cut(s) 541
MalI GATC 4 cut(s) 14, 62, 533, 714
MbiI CCGCTC 1 cut(s) 1043
MboI GATC 4 cut(s) 12, 60, 531, 712
MboII GAAGA 5 cut(s) 60, 225, 512, 575, 1079
MflI RGATCY 1 cut(s) 712
MhlI GDGCHC 1 cut(s) 583
MluCI AATT 5 cut(s) 242, 507, 638, 694, 827
MlyI GAGTC 1 cut(s) 481
MmeI TCCRAC 1 cut(s) 1064
MreI CGCCGGCG 1 cut(s) 77
MroI TCCGGA 1 cut(s) 265
MroNI GCCGGC 1 cut(s) 77
MroXI GAANNNNTTC 2 cut(s) 697, 827
MseI TTAA 4 cut(s) 171, 609, 672, 1055
MspA1I CMGCKG 1 cut(s) 923
MspCI CTTAAG 1 cut(s) 608
MspI CCGG 4 cut(s) 39, 78, 266, 1059
MspR9I CCNGG 1 cut(s) 552
MvaI CCWGG 1 cut(s) 552
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 5 cut(s) 187, 296, 599, 716, 1118
NaeI GCCGGC 1 cut(s) 79
NdeI CATATG 2 cut(s) 703, 882
NdeII GATC 4 cut(s) 12, 60, 531, 712
NgoMIV GCCGGC 1 cut(s) 77
NlaIII CATG 5 cut(s) 437, 473, 497, 635, 1002
NlaIV GGNNCC 2 cut(s) 26, 219
NmeAIII GCCGAG 2 cut(s) 96, 1138
NspI RCATGY 2 cut(s) 437, 635
PaeI GCATGC 1 cut(s) 635
PagI TCATGA 1 cut(s) 469
PceI AGGCCT 1 cut(s) 249
PciSI GCTCTTC 2 cut(s) 43, 588
PdiI GCCGGC 1 cut(s) 79
PdmI GAANNNNTTC 2 cut(s) 697, 827
PfeI GAWTC 4 cut(s) 42, 88, 415, 926
PinAI ACCGGT 1 cut(s) 1058
PkrI GCNGC 6 cut(s) 156, 604, 619, 733, 741, 1120
PleI GAGTC 1 cut(s) 481
PmaCI CACGTG 1 cut(s) 542
PmlI CACGTG 1 cut(s) 542
PpsI GAGTC 1 cut(s) 481
Ppu21I YACGTR 1 cut(s) 542
Psp124BI GAGCTC 1 cut(s) 583
Psp6I CCWGG 1 cut(s) 550
PspCI CACGTG 1 cut(s) 542
PspGI CCWGG 1 cut(s) 550
PspN4I GGNNCC 2 cut(s) 26, 219
PspPI GGNCC 5 cut(s) 25, 298, 323, 683, 886
PstI CTGCAG 1 cut(s) 744
PsuI RGATCY 1 cut(s) 712
RsaI GTAC 3 cut(s) 835, 958, 997
RsaNI GTAC 3 cut(s) 834, 957, 996
SacI GAGCTC 1 cut(s) 583
SapI GCTCTTC 2 cut(s) 43, 588
SaqAI TTAA 4 cut(s) 171, 609, 672, 1055
SatI GCNGC 6 cut(s) 155, 603, 618, 732, 740, 1119
Sau3AI GATC 4 cut(s) 12, 60, 531, 712
Sau96I GGNCC 5 cut(s) 25, 298, 323, 683, 886
ScaI AGTACT 1 cut(s) 835
SchI GAGTC 1 cut(s) 481
ScrFI CCNGG 1 cut(s) 552
SduI GDGCHC 1 cut(s) 583
SfaNI GCATC 5 cut(s) 235, 265, 350, 697, 895
SfcI CTRYAG 1 cut(s) 740
SgrAI CRCCGGYG 1 cut(s) 77
SinI GGWCC 1 cut(s) 25
SmlI CTYRAG 1 cut(s) 608
SmoI CTYRAG 1 cut(s) 608
SphI GCATGC 1 cut(s) 635
Sse9I AATT 5 cut(s) 242, 507, 638, 694, 827
SseBI AGGCCT 1 cut(s) 249
SsiI CCGC 9 cut(s) 5, 83, 154, 710, 921, 986, 1030, 1043, 1126
SspMI CTAG 1 cut(s) 786
SstI GAGCTC 1 cut(s) 583
StuI AGGCCT 1 cut(s) 249
StyD4I CCNGG 1 cut(s) 550
StyI CCWWGG 3 cut(s) 16, 425, 512
TaaI ACNGT 4 cut(s) 146, 384, 574, 995
TaiI ACGT 1 cut(s) 544
TaqI TCGA 3 cut(s) 304, 312, 1048
TasI AATT 5 cut(s) 242, 507, 638, 694, 827
TatI WGTACW 2 cut(s) 833, 995
TauI GCSGC 1 cut(s) 157
TfiI GAWTC 4 cut(s) 42, 88, 415, 926
Tru1I TTAA 4 cut(s) 171, 609, 672, 1055
Tru9I TTAA 4 cut(s) 171, 609, 672, 1055
TscAI CASTG 5 cut(s) 191, 577, 998, 1104, 1114
TseI GCWGC 5 cut(s) 602, 617, 731, 739, 1118
TspDTI ATGAA 2 cut(s) 575, 690
TspRI CASTG 5 cut(s) 191, 577, 998, 1104, 1114
Vha464I CTTAAG 1 cut(s) 608
VpaK11BI GGWCC 1 cut(s) 25
XapI RAATTY 2 cut(s) 507, 827
XceI RCATGY 2 cut(s) 437, 635
XmiI GTMKAC 2 cut(s) 849, 1162
XmnI GAANNNNTTC 2 cut(s) 697, 827
XspI CTAG 1 cut(s) 786
ZrmI AGTACT 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.