Rorug03G0144300

Mitochondrial fission protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003516
Physical Location & Seq
Reverse (-)
11841363 .. 11841830
468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0144300.1

Sequence Viewer

Length: 468 bp
ATGGCAGATGTGATGGAAGGACTTGCTACGGAGTTGACGTGCTCGGATTCAACCCTGGTTTTGTTGGGGGCGGCAGGGGCTGCTGCTTTTCAGGATGGTAATTGGTATATGGTAGGGCGGTTGTTGGCACCAAAAGCAACGTTGCCGGGATTCAAAGGCACCATATCCACCATCCGGCATATCAGGGCAGGGCTCACTATTCAGGATGCAGGAGATCGCTTTGTCTTCCAATTCGAGAGTGAGGCTATACGCAATCGAATTTTTCATAGGGGACCCTGGTTCTATCGAAACACCATGTTGGTGGTGGGAGAGTATGACGGCCTCAGTCCGGTGGAGGAGGTGCCTCTGAACATGATGGAAACCTGGGTTGCAGTTAAGGGACTACCTTTTGCTCTGAGAAACAAGACGGCGTTGTCCATGGTGGGATCGCACTCGGAAGGGTGCTCCGGTTGGATCCGAACACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mito_fiss_Elm1

Protein Analysis

155

Amino Acids

17.0

Weight (kDa)

6.28

Isoelectric Point (pI)

35.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 34 - 103 8e-07 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 412
AccB1I GGYRCC 3 cut(s) 127, 158, 340
AciI CCGC 2 cut(s) 71, 118
AclI AACGTT 1 cut(s) 140
AclWI GGATC 3 cut(s) 433, 448, 461
AcsI RAATTY 1 cut(s) 258
AfiI CCNNNNNNNGG 2 cut(s) 174, 328
AgsI TTSAA 2 cut(s) 51, 154
AjiI CACGTC 1 cut(s) 39
AjnI CCWGG 3 cut(s) 54, 275, 362
Alw21I GWGCWC 2 cut(s) 44, 446
AlwI GGATC 3 cut(s) 433, 448, 461
AlwNI CAGNNNCTG 1 cut(s) 80
AoxI GGCC 1 cut(s) 319
ApeKI GCWGC 2 cut(s) 80, 83
ApoI RAATTY 1 cut(s) 258
AspS9I GGNCC 1 cut(s) 272
AsuC2I CCSGG 1 cut(s) 147
AvaII GGWCC 1 cut(s) 272
BamHI GGATCC 1 cut(s) 453
BanI GGYRCC 3 cut(s) 127, 158, 340
BanII GRGCYC 1 cut(s) 195
BbsI GAAGAC 1 cut(s) 217
Bbv12I GWGCWC 2 cut(s) 44, 446
BbvI GCAGC 2 cut(s) 67, 70
BccI CCATC 4 cut(s) 7, 89, 179, 349
BceAI ACGGC 2 cut(s) 334, 423
BciT130I CCWGG 3 cut(s) 56, 277, 364
BcnI CCSGG 1 cut(s) 147
BisI GCNGC 3 cut(s) 72, 81, 84
BlsI GCNGC 3 cut(s) 73, 82, 85
Bme1390I CCNGG 4 cut(s) 56, 147, 277, 364
Bme18I GGWCC 1 cut(s) 272
BmgBI CACGTC 1 cut(s) 39
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 6 cut(s) 129, 160, 273, 274, 342, 455
BmrFI CCNGG 4 cut(s) 56, 147, 277, 364
BmsI GCATC 1 cut(s) 196
BpiI GAAGAC 1 cut(s) 217
BpuMI CCSGG 1 cut(s) 147
BsaJI CCNNGG 4 cut(s) 54, 275, 363, 417
BsaWI WCCGGW 2 cut(s) 328, 446
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 328
BseBI CCWGG 3 cut(s) 56, 277, 364
BseDI CCNNGG 4 cut(s) 54, 275, 363, 417
BseGI GGATG 3 cut(s) 100, 171, 211
BseLI CCNNNNNNNGG 2 cut(s) 174, 328
BseMII CTCAG 2 cut(s) 337, 386
BseRI GAGGAG 1 cut(s) 350
BseXI GCAGC 2 cut(s) 67, 70
BshFI GGCC 1 cut(s) 321
BshNI GGYRCC 3 cut(s) 127, 158, 340
BsiHKAI GWGCWC 2 cut(s) 44, 446
BsiSI CCGG 4 cut(s) 146, 175, 329, 447
BslFI GGGAC 2 cut(s) 285, 393
BslI CCNNNNNNNGG 2 cut(s) 174, 328
BsmFI GGGAC 2 cut(s) 285, 393
BsnI GGCC 1 cut(s) 321
Bsp1286I GDGCHC 3 cut(s) 44, 195, 446
Bsp143I GATC 3 cut(s) 214, 425, 453
Bsp19I CCATGG 1 cut(s) 417
BspACI CCGC 2 cut(s) 71, 118
BspANI GGCC 1 cut(s) 321
BspCNI CTCAG 2 cut(s) 336, 387
BspLI GGNNCC 6 cut(s) 129, 160, 273, 274, 342, 455
BspPI GGATC 3 cut(s) 433, 448, 461
BspT107I GGYRCC 3 cut(s) 127, 158, 340
BssECI CCNNGG 4 cut(s) 54, 275, 363, 417
BssMI GATC 3 cut(s) 214, 425, 453
BssT1I CCWWGG 1 cut(s) 417
Bst2UI CCWGG 3 cut(s) 56, 277, 364
BstAPI GCANNNNNTGC 1 cut(s) 80
BstDEI CTNAG 2 cut(s) 323, 395
BstDSI CCRYGG 1 cut(s) 417
BstF5I GGATG 3 cut(s) 100, 171, 211
BstKTI GATC 3 cut(s) 217, 428, 456
BstMBI GATC 3 cut(s) 214, 425, 453
BstMWI GCNNNNNNNGC 3 cut(s) 77, 80, 134
BstNI CCWGG 3 cut(s) 56, 277, 364
BstSCI CCNGG 4 cut(s) 54, 145, 275, 362
BstV1I GCAGC 2 cut(s) 67, 70
BstV2I GAAGAC 1 cut(s) 217
BstX2I RGATCY 1 cut(s) 453
BstXI CCANNNNNNTGG 1 cut(s) 301
BstYI RGATCY 1 cut(s) 453
BsuRI GGCC 1 cut(s) 321
BtgI CCRYGG 1 cut(s) 417
BtrI CACGTC 1 cut(s) 39
BtsCI GGATG 3 cut(s) 100, 171, 211
CaiI CAGNNNCTG 1 cut(s) 80
Cfr13I GGNCC 1 cut(s) 272
CviAII CATG 3 cut(s) 295, 352, 418
CviJI RGCY 4 cut(s) 80, 193, 245, 321
CviKI_1 RGCY 4 cut(s) 80, 193, 245, 321
DdeI CTNAG 2 cut(s) 323, 395
DpnI GATC 3 cut(s) 216, 427, 455
DpnII GATC 3 cut(s) 214, 425, 453
DrdI GACNNNNNNGTC 1 cut(s) 412
DseDI GACNNNNNNGTC 1 cut(s) 412
Eco130I CCWWGG 1 cut(s) 417
Eco24I GRGCYC 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 272
EcoRII CCWGG 3 cut(s) 54, 275, 362
EcoT14I CCWWGG 1 cut(s) 417
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 417
FaeI CATG 3 cut(s) 298, 355, 421
FaqI GGGAC 2 cut(s) 285, 393
FatI CATG 3 cut(s) 294, 351, 417
Fnu4HI GCNGC 3 cut(s) 72, 81, 84
FokI GGATG 3 cut(s) 107, 158, 218
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 3 cut(s) 72, 81, 84
GluI GCNGC 3 cut(s) 72, 81, 84
HaeIII GGCC 1 cut(s) 321
HapII CCGG 4 cut(s) 146, 175, 329, 447
Hin1II CATG 3 cut(s) 298, 355, 421
HincII GTYRAC 1 cut(s) 36
HindII GTYRAC 1 cut(s) 36
HinfI GANTC 2 cut(s) 47, 150
HpaII CCGG 4 cut(s) 146, 175, 329, 447
Hpy166II GTNNAC 1 cut(s) 36
Hpy188I TCNGA 5 cut(s) 46, 348, 396, 436, 458
Hpy188III TCNNGA 3 cut(s) 92, 203, 235
Hpy8I GTNNAC 1 cut(s) 36
HpyAV CCTTC 2 cut(s) 11, 431
HpyCH4IV ACGT 2 cut(s) 38, 140
HpyCH4V TGCA 2 cut(s) 209, 371
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 80, 134
HpyF3I CTNAG 2 cut(s) 323, 395
HpySE526I ACGT 2 cut(s) 38, 140
Hsp92II CATG 3 cut(s) 298, 355, 421
KflI GGGWCCC 1 cut(s) 272
Kzo9I GATC 3 cut(s) 214, 425, 453
LmnI GCTCC 1 cut(s) 449
Lsp1109I GCAGC 2 cut(s) 67, 70
LweI GCATC 1 cut(s) 196
MaeII ACGT 2 cut(s) 38, 140
MalI GATC 3 cut(s) 216, 427, 455
MboI GATC 3 cut(s) 214, 425, 453
MboII GAAGA 1 cut(s) 217
MflI RGATCY 1 cut(s) 453
MhlI GDGCHC 3 cut(s) 44, 195, 446
MluCI AATT 3 cut(s) 100, 230, 258
MmeI TCCRAC 1 cut(s) 431
MnlI CCTC 5 cut(s) 235, 328, 331, 332, 354
MseI TTAA 2 cut(s) 375, 466
MslI CAYNNNNRTG 1 cut(s) 299
MspI CCGG 4 cut(s) 146, 175, 329, 447
MspR9I CCNGG 4 cut(s) 56, 147, 277, 364
MvaI CCWGG 3 cut(s) 56, 277, 364
MwoI GCNNNNNNNGC 3 cut(s) 77, 80, 134
NciI CCSGG 1 cut(s) 147
NcoI CCATGG 1 cut(s) 417
NdeII GATC 3 cut(s) 214, 425, 453
NlaIII CATG 3 cut(s) 298, 355, 421
NlaIV GGNNCC 6 cut(s) 129, 160, 273, 274, 342, 455
PcsI WCGNNNNNNNCGW 1 cut(s) 35
PfeI GAWTC 2 cut(s) 47, 150
PkrI GCNGC 3 cut(s) 73, 82, 85
PpuMI RGGWCCY 1 cut(s) 272
Psp1406I AACGTT 1 cut(s) 140
Psp5II RGGWCCY 1 cut(s) 272
Psp6I CCWGG 3 cut(s) 54, 275, 362
PspGI CCWGG 3 cut(s) 54, 275, 362
PspN4I GGNNCC 6 cut(s) 129, 160, 273, 274, 342, 455
PspPI GGNCC 1 cut(s) 272
PspPPI RGGWCCY 1 cut(s) 272
PstNI CAGNNNCTG 1 cut(s) 80
PsuI RGATCY 1 cut(s) 453
RseI CAYNNNNRTG 1 cut(s) 299
SaqAI TTAA 2 cut(s) 375, 466
SatI GCNGC 3 cut(s) 72, 81, 84
Sau3AI GATC 3 cut(s) 214, 425, 453
Sau96I GGNCC 1 cut(s) 272
ScrFI CCNGG 4 cut(s) 56, 147, 277, 364
SduI GDGCHC 3 cut(s) 44, 195, 446
SetI ASST 5 cut(s) 41, 143, 342, 365, 388
SfaNI GCATC 1 cut(s) 196
SinI GGWCC 1 cut(s) 272
SmiMI CAYNNNNRTG 1 cut(s) 299
Sse9I AATT 3 cut(s) 100, 230, 258
SsiI CCGC 2 cut(s) 71, 118
StyD4I CCNGG 4 cut(s) 54, 145, 275, 362
StyI CCWWGG 1 cut(s) 417
TaiI ACGT 2 cut(s) 41, 143
TaqI TCGA 3 cut(s) 234, 256, 286
TasI AATT 3 cut(s) 100, 230, 258
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 2 cut(s) 47, 150
Tru1I TTAA 2 cut(s) 375, 466
Tru9I TTAA 2 cut(s) 375, 466
TseI GCWGC 2 cut(s) 80, 83
TspDTI ATGAA 1 cut(s) 254
TspGWI ACGGA 1 cut(s) 44
VpaK11BI GGWCC 1 cut(s) 272
XapI RAATTY 1 cut(s) 258
XcmI CCANNNNNNNNNTGG 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.