Rorug03G0144200

Mitochondrial fission protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003516
Physical Location & Seq
Reverse (-)
11822551 .. 11826309
3759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0144200.1

Sequence Viewer

Length: 1389 bp
ATGGCGGCATCGAGGAGGCTCCGGGACCTGCAATCGCAGCCGGGAAACAAGATCTGCGTGGACTGCTCGCAGAAGAATCCTCAATGGGCTTCGGTCTCCTACGGCGTCTTCATGTGCCTAGAGTGCTCCGGCAAGCACCGCGGCCTCGGCGTCCACATCTCCTTCGTCAGATCCGTGACCATGGACTCCTGGTCCGAGATCCAAATCAAGAAGATGGAGGCCGGCGGCAACGAGAGGCTCAACGCCTTCCTCGCCAGCTACGGCGTCTCTAAGGAGACCGATATCGTCACCAAGTACAACACCAACGCTGCCAGCGTCTACCGGGATCGCATTCAAGCCCTGGCCGAGGGTCGGCCTTGGCGGGACCCGCCGGTTGTGAAGGAGAGCGTCGGGGGGTTTGGGAAGACGAAGCCGCCTCTGGCTCACGGCGGAGGAGGTAGCAGGGGAGGCGGTGGCGGAGGCAACAATGGGGGTTGGGATAATTGGGACGACGACGGGTTCAGATCGAACGATATAAGGCGGAATCAGTCCACCGGCGATGTTAGAGGCGTGGGCGGCGGCGGAGGGATGCCGGCGAGGTCGAGATCGACAGAGGACATCATGCGATCGCAATTCGAGGCTTCCGCGGCGAACAAGGAGAGCTTCTTCGCCAGGAAGATGGCGGAGAATGAGTCCAGGCCGGACGGACTCCCACCGTCGCAAGGCGGCAAGTATGTCGGGTTCGGGTCAAGCCCGGCACCTTCTCAGAATAGTTATAATAACAATTCGCAAGGAGATGTGTTATCTGTTGTTTCTCAGGGTTTTGGTAGGCTATCTTTGGTTGCTGCATCTGCAGCTCAATCAGCTGCAACTGTAGTACAGGCAGGCACAAAAGAGATCACCTCTAAGGTGAAAGAGGGTGGCTATGACTACAAGGTGAATGAAACCGTTAATGTTGTTACTGCAAAGACGTCAGAGATAGGACAAAGAACTTGGGGGATTATGAAAGGGGTCATGGCAATGGCATCCCAGAAGGTTGAAGAGTATGCTAAAGAAGGAAACTGGAACACTGACAACTGGCAACGAAATGATAGCGAGCAAAATGGATATTATCAAGACTTTAATAAACAAGAGAATTCAGGATGGAATACTTCTTCTGGACGGGGGCAGTCCTCGTCAAGTGGACATAATAACTCTTATAACTCGAATTCTTGGGATGATTGGGACACCAAAGACAATAGGAAGGAAAGCTCTACGAAGGGAATGGGTTCCAATAGCAGTGATGGTTGGGCTGGCTGGGATGATGGCAAGGATGATGGATTTGATAGTGGCTACCAGAGTGCACCTACGAAAGGTGTTGGTCACAATGGGAAATCTGATTCAACATGGACTGGTGGAGGTTTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mito_fiss_Elm1

Protein Analysis

462

Amino Acids

49.64

Weight (kDa)

6.98

Isoelectric Point (pI)

50.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 5 - 110 3.9e-35 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 756, 1179
AatII GACGTC 1 cut(s) 953
Acc36I ACCTGC 1 cut(s) 36
AccB1I GGYRCC 1 cut(s) 736
AccI GTMKAC 1 cut(s) 318
AccII CGCG 2 cut(s) 141, 626
AclWI GGATC 3 cut(s) 165, 193, 333
AcoI YGGCCR 1 cut(s) 342
AcsI RAATTY 2 cut(s) 1114, 1186
AcyI GRCGYC 4 cut(s) 105, 150, 264, 950
AfaI GTAC 2 cut(s) 296, 858
AfiI CCNNNNNNNGG 4 cut(s) 346, 351, 701, 1331
AgsI TTSAA 3 cut(s) 335, 1019, 1362
AhdI GACNNNNNGTC 1 cut(s) 190
AjnI CCWGG 4 cut(s) 188, 339, 650, 674
AluBI AGCT 5 cut(s) 258, 642, 836, 845, 1230
AluI AGCT 5 cut(s) 258, 642, 836, 845, 1230
Alw21I GWGCWC 2 cut(s) 128, 1324
Alw26I GTCTC 3 cut(s) 100, 269, 271
Alw44I GTGCAC 1 cut(s) 1320
AlwI GGATC 3 cut(s) 165, 193, 333
AoxI GGCC 5 cut(s) 142, 219, 342, 353, 677
ApaLI GTGCAC 1 cut(s) 1320
ApeKI GCWGC 5 cut(s) 37, 308, 824, 833, 845
ApoI RAATTY 2 cut(s) 1114, 1186
AsiSI GCGATCGC 1 cut(s) 608
Asp700I GAANNNNTTC 1 cut(s) 1246
AspS9I GGNCC 3 cut(s) 25, 192, 364
AsuC2I CCSGG 4 cut(s) 23, 42, 323, 734
AsuHPI GGTGA 4 cut(s) 280, 871, 901, 928
AvaII GGWCC 3 cut(s) 25, 192, 364
BaeGI GKGCMC 1 cut(s) 1324
BanI GGYRCC 1 cut(s) 736
BbsI GAAGAC 2 cut(s) 100, 410
Bbv12I GWGCWC 2 cut(s) 128, 1324
BbvI GCAGC 5 cut(s) 49, 295, 811, 832, 845
BccI CCATC 6 cut(s) 208, 652, 1116, 1256, 1277, 1289
BceAI ACGGC 3 cut(s) 118, 277, 442
BcgI CGANNNNNNTGC 2 cut(s) 697, 731
BciT130I CCWGG 4 cut(s) 190, 341, 652, 676
BcnI CCSGG 4 cut(s) 23, 42, 323, 734
BcoDI GTCTC 3 cut(s) 100, 269, 271
BfaI CTAG 1 cut(s) 119
BfmI CTRYAG 2 cut(s) 831, 852
BfuAI ACCTGC 1 cut(s) 36
BglII AGATCT 1 cut(s) 51
Bme1390I CCNGG 8 cut(s) 23, 42, 190, 323, 341, 652, 676, 734
Bme18I GGWCC 3 cut(s) 25, 192, 364
BmeRI GACNNNNNGTC 1 cut(s) 190
BmgT120I GGNCC 3 cut(s) 25, 192, 364
BmiI GGNNCC 6 cut(s) 20, 26, 365, 366, 738, 1249
BmrFI CCNGG 8 cut(s) 23, 42, 190, 323, 341, 652, 676, 734
BmsI GCATC 4 cut(s) 17, 558, 836, 1013
BpiI GAAGAC 2 cut(s) 100, 410
BplI GAGNNNNNCTC 2 cut(s) 866, 898
BpuMI CCSGG 4 cut(s) 23, 42, 323, 734
BsaBI GATNNNNATC 1 cut(s) 203
BsaHI GRCGYC 4 cut(s) 105, 150, 264, 950
BsaI GGTCTC 2 cut(s) 100, 269
BsaJI CCNNGG 7 cut(s) 139, 145, 180, 339, 345, 356, 624
BsaXI ACNNNNNCTCC 2 cut(s) 656, 686
Bsc4I CCNNNNNNNGG 4 cut(s) 346, 351, 701, 1331
Bse118I RCCGGY 4 cut(s) 221, 370, 533, 571
Bse1I ACTGG 3 cut(s) 1046, 1061, 1375
Bse3DI GCAATG 1 cut(s) 1005
Bse8I GATNNNNATC 1 cut(s) 203
BseBI CCWGG 4 cut(s) 190, 341, 652, 676
BseDI CCNNGG 7 cut(s) 139, 145, 180, 339, 345, 356, 624
BseGI GGATG 6 cut(s) 573, 1004, 1127, 1201, 1285, 1297
BseJI GATNNNNATC 1 cut(s) 203
BseLI CCNNNNNNNGG 4 cut(s) 346, 351, 701, 1331
BseMI GCAATG 1 cut(s) 1005
BseMII CTCAG 2 cut(s) 758, 809
BseNI ACTGG 3 cut(s) 1046, 1061, 1375
BseRI GAGGAG 2 cut(s) 28, 447
BseSI GKGCMC 1 cut(s) 1324
BseXI GCAGC 5 cut(s) 49, 295, 811, 832, 845
BseYI CCCAGC 1 cut(s) 1275
Bsh1236I CGCG 2 cut(s) 141, 626
Bsh1285I CGRYCG 1 cut(s) 608
BshFI GGCC 5 cut(s) 144, 221, 344, 355, 679
BshNI GGYRCC 1 cut(s) 736
BsiEI CGRYCG 1 cut(s) 608
BsiHKAI GWGCWC 2 cut(s) 128, 1324
BslFI GGGAC 4 cut(s) 38, 377, 500, 1217
BslI CCNNNNNNNGG 4 cut(s) 346, 351, 701, 1331
BsmAI GTCTC 3 cut(s) 100, 269, 271
BsmBI CGTCTC 1 cut(s) 271
BsmFI GGGAC 4 cut(s) 38, 377, 500, 1217
BsmI GAATGC 1 cut(s) 330
BsnI GGCC 5 cut(s) 144, 221, 344, 355, 679
Bso31I GGTCTC 2 cut(s) 100, 269
Bsp1286I GDGCHC 2 cut(s) 128, 1324
Bsp143I GATC 8 cut(s) 51, 170, 198, 325, 503, 584, 605, 876
Bsp19I CCATGG 1 cut(s) 180
BspANI GGCC 5 cut(s) 144, 221, 344, 355, 679
BspCNI CTCAG 2 cut(s) 757, 808
BspFNI CGCG 2 cut(s) 141, 626
BspLI GGNNCC 6 cut(s) 20, 26, 365, 366, 738, 1249
BspMAI CTGCAG 1 cut(s) 835
BspMI ACCTGC 1 cut(s) 36
BspPI GGATC 3 cut(s) 165, 193, 333
BspT107I GGYRCC 1 cut(s) 736
BspTNI GGTCTC 2 cut(s) 100, 269
BsrDI GCAATG 1 cut(s) 1005
BsrFI RCCGGY 4 cut(s) 221, 370, 533, 571
BsrI ACTGG 3 cut(s) 1046, 1061, 1375
BssAI RCCGGY 4 cut(s) 221, 370, 533, 571
BssECI CCNNGG 7 cut(s) 139, 145, 180, 339, 345, 356, 624
BssMI GATC 8 cut(s) 51, 170, 198, 325, 503, 584, 605, 876
BssNI GRCGYC 4 cut(s) 105, 150, 264, 950
BssT1I CCWWGG 2 cut(s) 180, 356
Bst2UI CCWGG 4 cut(s) 190, 341, 652, 676
Bst4CI ACNGT 3 cut(s) 696, 853, 928
Bst6I CTCTTC 1 cut(s) 1014
BstACI GRCGYC 4 cut(s) 105, 150, 264, 950
BstC8I GCNNGC 9 cut(s) 68, 134, 223, 256, 313, 573, 865, 1076, 1273
BstDEI CTNAG 4 cut(s) 270, 744, 795, 885
BstDSI CCRYGG 3 cut(s) 139, 180, 624
BstENI CCTNNNNNAGG 2 cut(s) 344, 1329
BstF5I GGATG 6 cut(s) 573, 1004, 1127, 1201, 1285, 1297
BstFNI CGCG 2 cut(s) 141, 626
BstKTI GATC 8 cut(s) 54, 173, 201, 328, 506, 587, 608, 879
BstMAI GTCTC 3 cut(s) 100, 269, 271
BstMBI GATC 8 cut(s) 51, 170, 198, 325, 503, 584, 605, 876
BstMCI CGRYCG 1 cut(s) 608
BstNI CCWGG 4 cut(s) 190, 341, 652, 676
BstSCI CCNGG 8 cut(s) 21, 40, 188, 321, 339, 650, 674, 732
BstSFI CTRYAG 2 cut(s) 831, 852
BstSLI GKGCMC 1 cut(s) 1324
BstUI CGCG 2 cut(s) 141, 626
BstV1I GCAGC 5 cut(s) 49, 295, 811, 832, 845
BstV2I GAAGAC 2 cut(s) 100, 410
BstX2I RGATCY 3 cut(s) 51, 170, 198
BstXI CCANNNNNNTGG 1 cut(s) 658
BstYI RGATCY 3 cut(s) 51, 170, 198
BsuRI GGCC 5 cut(s) 144, 221, 344, 355, 679
BtgI CCRYGG 3 cut(s) 139, 180, 624
BtgZI GCGATG 1 cut(s) 552
BtsCI GGATG 6 cut(s) 573, 1004, 1127, 1201, 1285, 1297
BtsI GCAGTG 1 cut(s) 1264
BtsIMutI CAGTG 2 cut(s) 1047, 1264
BveI ACCTGC 1 cut(s) 36
Cac8I GCNNGC 9 cut(s) 68, 134, 223, 256, 313, 573, 865, 1076, 1273
Cfr10I RCCGGY 4 cut(s) 221, 370, 533, 571
Cfr13I GGNCC 3 cut(s) 25, 192, 364
Cfr42I CCGCGG 2 cut(s) 142, 627
CseI GACGC 5 cut(s) 94, 139, 253, 304, 376
Csp6I GTAC 2 cut(s) 295, 857
CviAII CATG 5 cut(s) 112, 181, 601, 994, 1365
CviQI GTAC 2 cut(s) 295, 857
DdeI CTNAG 4 cut(s) 270, 744, 795, 885
DpnI GATC 8 cut(s) 53, 172, 200, 327, 505, 586, 607, 878
DpnII GATC 8 cut(s) 51, 170, 198, 325, 503, 584, 605, 876
DriI GACNNNNNGTC 1 cut(s) 190
EaeI YGGCCR 1 cut(s) 342
Eam1104I CTCTTC 1 cut(s) 1014
Eam1105I GACNNNNNGTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 1014
EciI GGCGGA 5 cut(s) 444, 471, 535, 576, 677
Eco130I CCWWGG 2 cut(s) 180, 356
Eco31I GGTCTC 2 cut(s) 100, 269
Eco32I GATATC 1 cut(s) 283
Eco47I GGWCC 3 cut(s) 25, 192, 364
EcoNI CCTNNNNNAGG 2 cut(s) 344, 1329
EcoO109I RGGNCCY 2 cut(s) 25, 364
EcoRI GAATTC 2 cut(s) 1114, 1186
EcoRII CCWGG 4 cut(s) 188, 339, 650, 674
EcoRV GATATC 1 cut(s) 283
EcoT14I CCWWGG 2 cut(s) 180, 356
ErhI CCWWGG 2 cut(s) 180, 356
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 5 cut(s) 115, 184, 604, 997, 1368
FalI AAGNNNNNCTT 2 cut(s) 626, 658
FaqI GGGAC 4 cut(s) 38, 377, 500, 1217
FatI CATG 5 cut(s) 111, 180, 600, 993, 1364
FauI CCCGC 2 cut(s) 354, 375
FblI GTMKAC 1 cut(s) 318
FokI GGATG 6 cut(s) 580, 991, 1134, 1208, 1292, 1304
FspBI CTAG 1 cut(s) 119
GsaI CCCAGC 1 cut(s) 1279
HaeIII GGCC 5 cut(s) 144, 221, 344, 355, 679
HgaI GACGC 5 cut(s) 94, 139, 253, 304, 376
Hin1I GRCGYC 4 cut(s) 105, 150, 264, 950
Hin1II CATG 5 cut(s) 115, 184, 604, 997, 1368
HinfI GANTC 6 cut(s) 76, 185, 523, 671, 687, 1358
HphI GGTGA 4 cut(s) 280, 871, 901, 928
Hpy166II GTNNAC 6 cut(s) 61, 154, 319, 531, 1163, 1322
Hpy188I TCNGA 6 cut(s) 170, 196, 503, 747, 955, 1357
Hpy188III TCNNGA 5 cut(s) 208, 582, 1094, 1119, 1137
Hpy8I GTNNAC 6 cut(s) 61, 154, 319, 531, 1163, 1322
Hpy99I CGWCG 4 cut(s) 392, 494, 497, 700
HpyAV CCTTC 8 cut(s) 172, 256, 373, 750, 1006, 1028, 1216, 1231
HpyCH4III ACNGT 3 cut(s) 696, 853, 928
HpyCH4IV ACGT 1 cut(s) 950
HpyCH4V TGCA 6 cut(s) 31, 827, 833, 848, 944, 1322
HpyF3I CTNAG 4 cut(s) 270, 744, 795, 885
HpySE526I ACGT 1 cut(s) 950
Hsp92I GRCGYC 4 cut(s) 105, 150, 264, 950
Hsp92II CATG 5 cut(s) 115, 184, 604, 997, 1368
KflI GGGWCCC 1 cut(s) 364
KroI GCCGGC 2 cut(s) 221, 571
KroNI GCCGGC 2 cut(s) 223, 573
KspI CCGCGG 2 cut(s) 142, 627
Kzo9I GATC 8 cut(s) 51, 170, 198, 325, 503, 584, 605, 876
LmnI GCTCC 2 cut(s) 24, 131
Lsp1109I GCAGC 5 cut(s) 49, 295, 811, 832, 845
LweI GCATC 4 cut(s) 17, 558, 836, 1013
MaeI CTAG 1 cut(s) 119
MaeII ACGT 1 cut(s) 950
MaeIII GTNAC 4 cut(s) 175, 286, 937, 1340
MalI GATC 8 cut(s) 53, 172, 200, 327, 505, 586, 607, 878
MboI GATC 8 cut(s) 51, 170, 198, 325, 503, 584, 605, 876
MboII GAAGA 8 cut(s) 85, 100, 223, 415, 637, 667, 1031, 1125
MflI RGATCY 3 cut(s) 51, 170, 198
MhlI GDGCHC 2 cut(s) 128, 1324
MluCI AATT 5 cut(s) 481, 611, 763, 1114, 1186
MlyI GAGTC 3 cut(s) 179, 680, 681
MroNI GCCGGC 2 cut(s) 221, 571
MroXI GAANNNNTTC 1 cut(s) 1246
MseI TTAA 2 cut(s) 930, 1101
MslI CAYNNNNRTG 1 cut(s) 998
MspA1I CMGCKG 3 cut(s) 141, 626, 845
MspR9I CCNGG 8 cut(s) 23, 42, 190, 323, 341, 652, 676, 734
Mva1269I GAATGC 1 cut(s) 330
MvaI CCWGG 4 cut(s) 190, 341, 652, 676
MvnI CGCG 2 cut(s) 141, 626
NaeI GCCGGC 2 cut(s) 223, 573
NciI CCSGG 4 cut(s) 23, 42, 323, 734
NcoI CCATGG 1 cut(s) 180
NdeII GATC 8 cut(s) 51, 170, 198, 325, 503, 584, 605, 876
NgoMIV GCCGGC 2 cut(s) 221, 571
NlaIII CATG 5 cut(s) 115, 184, 604, 997, 1368
NlaIV GGNNCC 6 cut(s) 20, 26, 365, 366, 738, 1249
NmeAIII GCCGAG 2 cut(s) 126, 370
NmuCI GTSAC 3 cut(s) 175, 286, 1340
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 312
PctI GAATGC 1 cut(s) 330
PdiI GCCGGC 2 cut(s) 223, 573
PdmI GAANNNNTTC 1 cut(s) 1246
PfeI GAWTC 3 cut(s) 76, 523, 1358
PfoI TCCNGGA 1 cut(s) 21
Ple19I CGATCG 1 cut(s) 608
PleI GAGTC 3 cut(s) 179, 679, 681
PpsI GAGTC 3 cut(s) 179, 679, 681
PpuMI RGGWCCY 2 cut(s) 25, 364
PsiI TTATAA 2 cut(s) 756, 1179
Psp5II RGGWCCY 2 cut(s) 25, 364
Psp6I CCWGG 4 cut(s) 188, 339, 650, 674
PspFI CCCAGC 1 cut(s) 1275
PspGI CCWGG 4 cut(s) 188, 339, 650, 674
PspN4I GGNNCC 6 cut(s) 20, 26, 365, 366, 738, 1249
PspPI GGNCC 3 cut(s) 25, 192, 364
PspPPI RGGWCCY 2 cut(s) 25, 364
PsrI GAACNNNNNNTAC 2 cut(s) 704, 736
PstI CTGCAG 1 cut(s) 835
PsuI RGATCY 3 cut(s) 51, 170, 198
PvuI CGATCG 1 cut(s) 608
PvuII CAGCTG 1 cut(s) 845
RgaI GCGATCGC 1 cut(s) 608
RsaI GTAC 2 cut(s) 296, 858
RsaNI GTAC 2 cut(s) 295, 857
RseI CAYNNNNRTG 1 cut(s) 998
SacII CCGCGG 2 cut(s) 142, 627
SaqAI TTAA 2 cut(s) 930, 1101
Sau3AI GATC 8 cut(s) 51, 170, 198, 325, 503, 584, 605, 876
Sau96I GGNCC 3 cut(s) 25, 192, 364
SchI GAGTC 3 cut(s) 179, 680, 681
ScrFI CCNGG 8 cut(s) 23, 42, 190, 323, 341, 652, 676, 734
SduI GDGCHC 2 cut(s) 128, 1324
SfaAI GCGATCGC 1 cut(s) 608
SfaNI GCATC 4 cut(s) 17, 558, 836, 1013
SfcI CTRYAG 2 cut(s) 831, 852
Sfr303I CCGCGG 2 cut(s) 142, 627
SgfI GCGATCGC 1 cut(s) 608
SgrAI CRCCGGYG 1 cut(s) 533
SgrBI CCGCGG 2 cut(s) 142, 627
SinI GGWCC 3 cut(s) 25, 192, 364
SmiMI CAYNNNNRTG 1 cut(s) 998
Sse9I AATT 5 cut(s) 481, 611, 763, 1114, 1186
SspMI CTAG 1 cut(s) 119
StyD4I CCNGG 8 cut(s) 21, 40, 188, 321, 339, 650, 674, 732
StyI CCWWGG 2 cut(s) 180, 356
TaaI ACNGT 3 cut(s) 696, 853, 928
TaiI ACGT 1 cut(s) 953
TaqI TCGA 6 cut(s) 11, 506, 581, 587, 615, 1184
TaqII GACCGA 2 cut(s) 82, 293
TasI AATT 5 cut(s) 481, 611, 763, 1114, 1186
TatI WGTACW 2 cut(s) 294, 856
TauI GCSGC 8 cut(s) 8, 144, 228, 415, 558, 561, 629, 708
TfiI GAWTC 3 cut(s) 76, 523, 1358
Tru1I TTAA 2 cut(s) 930, 1101
Tru9I TTAA 2 cut(s) 930, 1101
TscAI CASTG 2 cut(s) 1054, 1264
TseFI GTSAC 3 cut(s) 175, 286, 1340
TseI GCWGC 5 cut(s) 37, 308, 824, 833, 845
Tsp45I GTSAC 3 cut(s) 175, 286, 1340
TspDTI ATGAA 3 cut(s) 100, 936, 998
TspGWI ACGGA 2 cut(s) 163, 699
TspRI CASTG 2 cut(s) 1054, 1264
VneI GTGCAC 1 cut(s) 1320
VpaK11BI GGWCC 3 cut(s) 25, 192, 364
XagI CCTNNNNNAGG 2 cut(s) 344, 1329
XapI RAATTY 2 cut(s) 1114, 1186
XmiI GTMKAC 1 cut(s) 318
XmnI GAANNNNTTC 1 cut(s) 1246
XspI CTAG 1 cut(s) 119
ZraI GACGTC 1 cut(s) 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.