Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0006898

Physical Location & Seq

Genomic Coordinates Reverse (-)

16418580 .. 16422942

Gene Structure 4363 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1105600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1611 bp

ATGTCCATGGACCCAAACGCCGTGAAAACGGAGCTGGTCCTGATTCTCGACTATGGCTCCCAATACACCCACCTCATCACCCGCCGGATTCGATCCCTCTCCGTTTTCTCCCTCTGCATCTCCGGCACAAGCCCTCTCAAATCCATCACCGACCTCAACCCCCGCGTCGTAATCCTCTCTGGCGGGCCCCACTCCGTCCACACCCCGGACTCCCCCAGCTTCCCGGCCGGATTCACCGAGTGGGCCCAGGCCAACGGCGTGTTCGTGCTCGGCGTCTGCTACGGGCTTCAGTTGATTGTGCAGAGGCTCGGTGGAGTGGTTAAGGTTGGGGAGAAGCAGGAGTATGGAAGGATGGAGGTTGAGGTGGAGAGAAACACCGGGATTTTCGGGTCCAAGAAGGTTGGGGATAAGCAGGTGGTTTGGATGAGCCACGGTGATGAGGTGGCGAAACTGCCGGAAGGGTTTGAGGTCGTGGCGCGGAGCCAGCAGGGATCGGTGGCTGCAATTGAGAGTCCGGCTAGGAGGTTCTATGGGTTGCAGTACCACCCGGAGGTGACTCATTCGCCAGAAGGGATGGAGACGCTTCGATACTTTTTACTTGATATTTGTAAAGTCAGTGCAGGATGGAGTATGGAAAATGTATTGGATGAAGAAATAAAAGTGATTAAAAGCACAGTGGGGGCTGAAGATCATGTCATATGTGCGTTATCAGGAGGTGTCGATTCCACAGTTGCCGCAACTCTTGTTCATAAGGCAATTGGCGATAGGCTTCATTGTATCTTCGTTGACAATGGTTTATTGAGATTTAAGGAGAGAGAACGTGTGATGGAAACCTTTGAAAAAGATCTTCATTTGCCTGTTACTTGTGTGGATGCCACAGAACAATTTCTTAGTAAGCTTAAAGGTGTTGTTGATCCTGAGACAAAAAGGAAAATAATTGGAAGGGAGTTCATTTGCATTTTTGATGCTTTTGCACATGATTTGGAGCAAAAATTAGGGAGGAAACCTACTTACTTGGTCCAAGGGACCCTGTATCCAGATGTGATTGAATCTTGCCCACCACCTGGAAGTGGAAGGACCCACTCTCACACCATCAAGAGCCATCACAACGTTGGAGGGCTTCCCAAAGACATGAAACTGAAGCTCATTGAGCCTCTTAAACTTCTATTCAAGGATGAGGTTCGTGAATTAGGGAGGATCTTAGATGTTCCTCAGGGATTTCTCAAGCGCCATCCATTTCCTGGCCCTGGCCTTGCAGTACGAGTCTTGGGTGATGTAACTCAAGGCAATGCATTGGATATCCTCCGCCTGGTTGATGAGATATTCATACGATCAATCAAAGATGCTGGGTTGTACGATACAATCTGGCAAGCATTTGCGGTCTTTTTGCCTGTAAGATCTGTTGGGGTTCAGGGCGATCAAAGAACACATTCTCACGTTGTTGCCCTCAGAGCTGTAACAAGTCAAGATGGGATGACAGCTGACTGGTACTATTTTGAACATAAGTTCCTTGATGATGTGTCCCGAAAGATTTGCAATAGTGTTCGGGGTGTAAACAGAGTAGTTCAAGACATTACGTCAAAGCCCCCATCAACAATTGAGTGGGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003921 GO:0003922 GO:0006139 GO:0006163 GO:0006164 GO:0006177 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009163 GO:0009165 GO:0009167 GO:0009168 GO:0009259 GO:0009260 GO:0009987 GO:0016874 GO:0016879 GO:0016884 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0034404 GO:0034641 GO:0034654 GO:0042278 GO:0042451 GO:0042455 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0046037 GO:0046128 GO:0046129 GO:0046390 GO:0046483 GO:0055086 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:1901068 GO:1901070 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

ko00230 ko00983 ko01100 map00230 map00983 map01100

Pfam Domains

Protein Families

GATase GMP_synt_C NAD_synthase

Protein Analysis

537

Amino Acids

59.57

Weight (kDa)

6.56

Isoelectric Point (pI)

38.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase	PF00117	14 - 201	3.2e-36	Glutamine amidotransferase class-I
Peptidase_C26	PF07722	82 - 184	1.7e-09	Peptidase C26
GMP_synt_C	PF00958	445 - 535	1.7e-41	GMP synthase C terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006898)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63660 AT1G63660
fragaria_vesca	FvH4_5g27720 FvH4_5g27720
malus_domestica	MD06G1084500.v1.1 MD14G1105600.v1.1
prunus_persica	Prupe.5G100800_v2.0.a1
pyrus_communis	pycom06g08080 pycom14g10110
rosa_chinensis	RchiOBHm_Chr1g0356411 RchiOBHm_Chr7g0219471
rosa_laevigata	RLG00000002288 RLG00000005808 RLG00000011737
rosa_multiflora	Rmu_sc0003767.1_g000005 Rmu_sc0012443.1_g000008
rosa_roxburghii	Rroxscaffold_3G00240030 Rroxscaffold_5G00385240 Rroxscaffold_6G00391170
rosa_rugosa	Rorug07G0185400
rosa_samantha	Rh4DG422800 Rh7BG318200 Rh7CG345600 Rh7DG324200
rosa_wichuraiana	Rw1G013280 Rw7G027730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	403
Acc36I	ACCTGC	1 cut(s)	403
AccB7I	CCANNNNNTGG	2 cut(s)	1064, 1241
AccII	CGCG	2 cut(s)	165, 478
AciI	CCGC	7 cut(s)	82, 163, 183, 478, 735, 1306, 1379
AclI	AACGTT	1 cut(s)	1110
AclWI	GGATC	4 cut(s)	87, 499, 908, 1205
AcoI	YGGCCR	1 cut(s)	225
AcuI	CTGAAG	3 cut(s)	272, 705, 1160
AcyI	GRCGYC	1 cut(s)	273
AdeI	CACNNNGTG	1 cut(s)	240
AfaI	GTAC	4 cut(s)	542, 1260, 1355, 1490
AfiI	CCNNNNNNNGG	7 cut(s)	205, 550, 1064, 1070, 1241, 1247, 1309
AflIII	ACRYGT	1 cut(s)	820
AgsI	TTSAA	5 cut(s)	839, 1049, 1171, 1499, 1568
AhdI	GACNNNNNGTC	1 cut(s)	1576
AjnI	CCWGG	5 cut(s)	246, 1063, 1240, 1246, 1308
AjuI	GAANNNNNNNTTGG	2 cut(s)	245, 277
AloI	GAACNNNNNNTCC	2 cut(s)	1491, 1523
AluBI	AGCT	6 cut(s)	34, 219, 898, 1144, 1454, 1481
AluI	AGCT	6 cut(s)	34, 219, 898, 1144, 1454, 1481
Alw21I	GWGCWC	1 cut(s)	270
Alw26I	GTCTC	2 cut(s)	572, 914
AlwI	GGATC	4 cut(s)	87, 499, 908, 1205
AoxI	GGCC	6 cut(s)	185, 225, 243, 249, 1243, 1249
ApaI	GGGCCC	2 cut(s)	189, 247
ApeKI	GCWGC	1 cut(s)	500
Asp700I	GAANNNNTTC	2 cut(s)	885, 1429
AspLEI	GCGC	2 cut(s)	478, 1230
AsuC2I	CCSGG	4 cut(s)	206, 224, 379, 548
AsuHPI	GGTGA	6 cut(s)	70, 139, 226, 446, 565, 1283
AvaII	GGWCC	6 cut(s)	10, 37, 390, 1018, 1026, 1077
AxyI	CCTNAGG	1 cut(s)	1212
BaeGI	GKGCMC	2 cut(s)	189, 247
BanII	GRGCYC	2 cut(s)	189, 247
Bbv12I	GWGCWC	1 cut(s)	270
BbvI	GCAGC	1 cut(s)	487
BceAI	ACGGC	2 cut(s)	5, 271
BcgI	CGANNNNNNTGC	2 cut(s)	1515, 1549
BciT130I	CCWGG	5 cut(s)	248, 1065, 1242, 1248, 1310
BciVI	GTATCC	1 cut(s)	1044
BcnI	CCSGG	4 cut(s)	206, 224, 379, 548
BcoDI	GTCTC	2 cut(s)	572, 914
BfaI	CTAG	1 cut(s)	519
BfoI	RGCGCY	1 cut(s)	1231
BfuAI	ACCTGC	1 cut(s)	403
BfuI	GTATCC	1 cut(s)	1044
BglII	AGATCT	2 cut(s)	844, 1397
BisI	GCNGC	2 cut(s)	501, 735
BlsI	GCNGC	2 cut(s)	502, 736
Bme1390I	CCNGG	9 cut(s)	206, 224, 248, 379, 548, 1065, 1242, 1248, 1310
Bme18I	GGWCC	6 cut(s)	10, 37, 390, 1018, 1026, 1077
BmeRI	GACNNNNNGTC	1 cut(s)	1576
BmrFI	CCNGG	9 cut(s)	206, 224, 248, 379, 548, 1065, 1242, 1248, 1310
BmsI	GCATC	4 cut(s)	126, 862, 955, 1333
BpuEI	CTTGAG	2 cut(s)	1208, 1266
BpuMI	CCSGG	4 cut(s)	206, 224, 379, 548
BsaHI	GRCGYC	1 cut(s)	273
BsaJI	CCNNGG	6 cut(s)	6, 204, 246, 430, 1021, 1246
BsaXI	ACNNNNNCTCC	2 cut(s)	41, 71
Bsc4I	CCNNNNNNNGG	7 cut(s)	205, 550, 1064, 1070, 1241, 1247, 1309
Bse1I	ACTGG	1 cut(s)	1490
Bse21I	CCTNAGG	1 cut(s)	1212
Bse3DI	GCAATG	1 cut(s)	1294
BseBI	CCWGG	5 cut(s)	248, 1065, 1242, 1248, 1310
BseDI	CCNNGG	6 cut(s)	6, 204, 246, 430, 1021, 1246
BseGI	GGATG	9 cut(s)	357, 429, 579, 629, 652, 877, 1180, 1231, 1479
BseLI	CCNNNNNNNGG	7 cut(s)	205, 550, 1064, 1070, 1241, 1247, 1309
BseMI	GCAATG	1 cut(s)	1294
BseMII	CTCAG	3 cut(s)	909, 1226, 1462
BseNI	ACTGG	1 cut(s)	1490
BseSI	GKGCMC	2 cut(s)	189, 247
BseX3I	CGGCCG	1 cut(s)	225
BseXI	GCAGC	1 cut(s)	487
BseYI	CCCAGC	2 cut(s)	215, 1346
BsgI	GTGCAG	2 cut(s)	320, 639
Bsh1236I	CGCG	2 cut(s)	165, 478
Bsh1285I	CGRYCG	1 cut(s)	228
BshFI	GGCC	6 cut(s)	187, 227, 245, 251, 1245, 1251
BsiEI	CGRYCG	1 cut(s)	228
BsiHKAI	GWGCWC	1 cut(s)	270
BsiSI	CCGG	9 cut(s)	85, 123, 206, 224, 228, 378, 455, 515, 548
BslFI	GGGAC	2 cut(s)	1039, 1507
BslI	CCNNNNNNNGG	7 cut(s)	205, 550, 1064, 1070, 1241, 1247, 1309
BsmAI	GTCTC	2 cut(s)	572, 914
BsmBI	CGTCTC	1 cut(s)	572
BsmFI	GGGAC	2 cut(s)	1039, 1507
BsnI	GGCC	6 cut(s)	187, 227, 245, 251, 1245, 1251
Bsp120I	GGGCCC	2 cut(s)	185, 243
Bsp1286I	GDGCHC	3 cut(s)	189, 247, 270
Bsp143I	GATC	9 cut(s)	92, 491, 688, 844, 913, 1197, 1331, 1397, 1417
Bsp19I	CCATGG	1 cut(s)	6
BspACI	CCGC	7 cut(s)	82, 163, 183, 478, 735, 1306, 1379
BspANI	GGCC	6 cut(s)	187, 227, 245, 251, 1245, 1251
BspCNI	CTCAG	3 cut(s)	910, 1225, 1461
BspFNI	CGCG	2 cut(s)	165, 478
BspMI	ACCTGC	1 cut(s)	403
BspPI	GGATC	4 cut(s)	87, 499, 908, 1205
BsrDI	GCAATG	1 cut(s)	1294
BsrI	ACTGG	1 cut(s)	1490
BssECI	CCNNGG	6 cut(s)	6, 204, 246, 430, 1021, 1246
BssMI	GATC	9 cut(s)	92, 491, 688, 844, 913, 1197, 1331, 1397, 1417
BssNI	GRCGYC	1 cut(s)	273
BssT1I	CCWWGG	2 cut(s)	6, 1021
Bst2UI	CCWGG	5 cut(s)	248, 1065, 1242, 1248, 1310
Bst4CI	ACNGT	3 cut(s)	434, 676, 730
BstACI	GRCGYC	1 cut(s)	273
BstC8I	GCNNGC	3 cut(s)	185, 485, 1371
BstDEI	CTNAG	5 cut(s)	890, 918, 1201, 1212, 1448
BstDSI	CCRYGG	2 cut(s)	6, 430
BstF5I	GGATG	9 cut(s)	357, 429, 579, 629, 652, 877, 1180, 1231, 1479
BstFNI	CGCG	2 cut(s)	165, 478
BstH2I	RGCGCY	1 cut(s)	1231
BstHHI	GCGC	2 cut(s)	478, 1230
BstKTI	GATC	9 cut(s)	95, 494, 691, 847, 916, 1200, 1334, 1400, 1420
BstMAI	GTCTC	2 cut(s)	572, 914
BstMBI	GATC	9 cut(s)	92, 491, 688, 844, 913, 1197, 1331, 1397, 1417
BstMCI	CGRYCG	1 cut(s)	228
BstMWI	GCNNNNNNNGC	4 cut(s)	123, 484, 1150, 1451
BstNI	CCWGG	5 cut(s)	248, 1065, 1242, 1248, 1310
BstSCI	CCNGG	9 cut(s)	204, 222, 246, 377, 546, 1063, 1240, 1246, 1308
BstSLI	GKGCMC	2 cut(s)	189, 247
BstUI	CGCG	2 cut(s)	165, 478
BstV1I	GCAGC	1 cut(s)	487
BstX2I	RGATCY	3 cut(s)	844, 1197, 1397
BstYI	RGATCY	3 cut(s)	844, 1197, 1397
BstZI	CGGCCG	1 cut(s)	225
Bsu36I	CCTNAGG	1 cut(s)	1212
BsuI	GTATCC	1 cut(s)	1044
BsuRI	GGCC	6 cut(s)	187, 227, 245, 251, 1245, 1251
BtgI	CCRYGG	2 cut(s)	6, 430
BtsCI	GGATG	9 cut(s)	357, 429, 579, 629, 652, 877, 1180, 1231, 1479
BtsIMutI	CAGTG	2 cut(s)	622, 681
BveI	ACCTGC	1 cut(s)	403
Cac8I	GCNNGC	3 cut(s)	185, 485, 1371
CfoI	GCGC	2 cut(s)	478, 1230
CseI	GACGC	3 cut(s)	154, 262, 589
Csp6I	GTAC	4 cut(s)	541, 1259, 1354, 1489
CviAII	CATG	4 cut(s)	7, 692, 977, 1132
CviQI	GTAC	4 cut(s)	541, 1259, 1354, 1489
DdeI	CTNAG	5 cut(s)	890, 918, 1201, 1212, 1448
DpnI	GATC	9 cut(s)	94, 493, 690, 846, 915, 1199, 1333, 1399, 1419
DpnII	GATC	9 cut(s)	92, 491, 688, 844, 913, 1197, 1331, 1397, 1417
DraIII	CACNNNGTG	1 cut(s)	240
DriI	GACNNNNNGTC	1 cut(s)	1576
EaeI	YGGCCR	1 cut(s)	225
EagI	CGGCCG	1 cut(s)	225
Eam1105I	GACNNNNNGTC	1 cut(s)	1576
EciI	GGCGGA	1 cut(s)	1295
EclXI	CGGCCG	1 cut(s)	225
Eco130I	CCWWGG	2 cut(s)	6, 1021
Eco24I	GRGCYC	2 cut(s)	189, 247
Eco32I	GATATC	1 cut(s)	1300
Eco47I	GGWCC	6 cut(s)	10, 37, 390, 1018, 1026, 1077
Eco52I	CGGCCG	1 cut(s)	225
Eco57I	CTGAAG	3 cut(s)	272, 705, 1160
Eco81I	CCTNAGG	1 cut(s)	1212
EcoO109I	RGGNCCY	3 cut(s)	186, 1026, 1077
EcoRII	CCWGG	5 cut(s)	246, 1063, 1240, 1246, 1308
EcoRV	GATATC	1 cut(s)	1300
EcoT14I	CCWWGG	2 cut(s)	6, 1021
EcoT22I	ATGCAT	1 cut(s)	1294
EcoT38I	GRGCYC	2 cut(s)	189, 247
ErhI	CCWWGG	2 cut(s)	6, 1021
Esp3I	CGTCTC	1 cut(s)	572
FaeI	CATG	4 cut(s)	10, 695, 980, 1135
FaqI	GGGAC	2 cut(s)	1039, 1507
FatI	CATG	4 cut(s)	6, 691, 976, 1131
FauI	CCCGC	3 cut(s)	89, 170, 176
FauNDI	CATATG	1 cut(s)	698
Fnu4HI	GCNGC	2 cut(s)	501, 735
FokI	GGATG	9 cut(s)	364, 436, 586, 636, 659, 884, 1187, 1218, 1486
FriOI	GRGCYC	2 cut(s)	189, 247
Fsp4HI	GCNGC	2 cut(s)	501, 735
FspBI	CTAG	1 cut(s)	519
GlaI	GCGC	2 cut(s)	477, 1229
GluI	GCNGC	2 cut(s)	501, 735
GsaI	CCCAGC	2 cut(s)	219, 1350
HaeII	RGCGCY	1 cut(s)	1231
HaeIII	GGCC	6 cut(s)	187, 227, 245, 251, 1245, 1251
HapII	CCGG	9 cut(s)	85, 123, 206, 224, 228, 378, 455, 515, 548
HgaI	GACGC	3 cut(s)	154, 262, 589
HhaI	GCGC	2 cut(s)	478, 1230
Hin1I	GRCGYC	1 cut(s)	273
Hin1II	CATG	4 cut(s)	10, 695, 980, 1135
Hin6I	GCGC	2 cut(s)	476, 1228
HinP1I	GCGC	2 cut(s)	476, 1228
HincII	GTYRAC	1 cut(s)	787
HindII	GTYRAC	1 cut(s)	787
HindIII	AAGCTT	1 cut(s)	896
HinfI	GANTC	9 cut(s)	43, 88, 209, 231, 511, 556, 722, 1049, 1263
HpaII	CCGG	9 cut(s)	85, 123, 206, 224, 228, 378, 455, 515, 548
HphI	GGTGA	6 cut(s)	70, 139, 226, 446, 565, 1283
Hpy166II	GTNNAC	3 cut(s)	199, 787, 1555
Hpy188I	TCNGA	1 cut(s)	1451
Hpy8I	GTNNAC	3 cut(s)	199, 787, 1555
Hpy99I	CGWCG	1 cut(s)	170
HpyAV	CCTTC	6 cut(s)	342, 391, 452, 563, 936, 1068
HpyCH4III	ACNGT	3 cut(s)	434, 676, 730
HpyCH4IV	ACGT	4 cut(s)	820, 1110, 1437, 1577
HpyF10VI	GCNNNNNNNGC	4 cut(s)	123, 484, 1150, 1451
HpyF3I	CTNAG	5 cut(s)	890, 918, 1201, 1212, 1448
HpySE526I	ACGT	4 cut(s)	820, 1110, 1437, 1577
Hsp92I	GRCGYC	1 cut(s)	273
Hsp92II	CATG	4 cut(s)	10, 695, 980, 1135
HspAI	GCGC	2 cut(s)	476, 1228
KflI	GGGWCCC	1 cut(s)	1026
Kzo9I	GATC	9 cut(s)	92, 491, 688, 844, 913, 1197, 1331, 1397, 1417
LmnI	GCTCC	4 cut(s)	31, 62, 480, 985
Lsp1109I	GCAGC	1 cut(s)	487
LweI	GCATC	4 cut(s)	126, 862, 955, 1333
MaeI	CTAG	1 cut(s)	519
MaeII	ACGT	4 cut(s)	820, 1110, 1437, 1577
MaeIII	GTNAC	4 cut(s)	553, 859, 1276, 1456
MalI	GATC	9 cut(s)	94, 493, 690, 846, 915, 1199, 1333, 1399, 1419
MboI	GATC	9 cut(s)	92, 491, 688, 844, 913, 1197, 1331, 1397, 1417
MboII	GAAGA	4 cut(s)	662, 698, 772, 839
MfeI	CAATTG	3 cut(s)	504, 756, 1596
MflI	RGATCY	3 cut(s)	844, 1197, 1397
MhlI	GDGCHC	3 cut(s)	189, 247, 270
MluCI	AATT	7 cut(s)	504, 756, 884, 936, 992, 1187, 1596
MlyI	GAGTC	4 cut(s)	203, 520, 550, 1272
MmeI	TCCRAC	1 cut(s)	1093
Mph1103I	ATGCAT	1 cut(s)	1294
MroXI	GAANNNNTTC	2 cut(s)	885, 1429
MseI	TTAA	5 cut(s)	321, 666, 807, 900, 1158
MslI	CAYNNNNRTG	1 cut(s)	435
MspA1I	CMGCKG	1 cut(s)	1481
MspI	CCGG	9 cut(s)	85, 123, 206, 224, 228, 378, 455, 515, 548
MspR9I	CCNGG	9 cut(s)	206, 224, 248, 379, 548, 1065, 1242, 1248, 1310
MunI	CAATTG	3 cut(s)	504, 756, 1596
MvaI	CCWGG	5 cut(s)	248, 1065, 1242, 1248, 1310
MvnI	CGCG	2 cut(s)	165, 478
MwoI	GCNNNNNNNGC	4 cut(s)	123, 484, 1150, 1451
NciI	CCSGG	4 cut(s)	206, 224, 379, 548
NcoI	CCATGG	1 cut(s)	6
NdeI	CATATG	1 cut(s)	698
NdeII	GATC	9 cut(s)	92, 491, 688, 844, 913, 1197, 1331, 1397, 1417
NlaIII	CATG	4 cut(s)	10, 695, 980, 1135
NmeAIII	GCCGAG	1 cut(s)	249
NmuCI	GTSAC	1 cut(s)	553
NsiI	ATGCAT	1 cut(s)	1294
PaqCI	CACCTGC	1 cut(s)	403
PcsI	WCGNNNNNNNCGW	2 cut(s)	261, 270
PdmI	GAANNNNTTC	2 cut(s)	885, 1429
PfeI	GAWTC	5 cut(s)	43, 88, 231, 722, 1049
PflMI	CCANNNNNTGG	2 cut(s)	1064, 1241
PkrI	GCNGC	2 cut(s)	502, 736
PleI	GAGTC	4 cut(s)	203, 519, 550, 1271
PpsI	GAGTC	4 cut(s)	203, 519, 550, 1271
PpuMI	RGGWCCY	2 cut(s)	1026, 1077
Psp1406I	AACGTT	1 cut(s)	1110
Psp5II	RGGWCCY	2 cut(s)	1026, 1077
Psp6I	CCWGG	5 cut(s)	246, 1063, 1240, 1246, 1308
PspFI	CCCAGC	2 cut(s)	215, 1346
PspGI	CCWGG	5 cut(s)	246, 1063, 1240, 1246, 1308
PspOMI	GGGCCC	2 cut(s)	185, 243
PspPPI	RGGWCCY	2 cut(s)	1026, 1077
PsuI	RGATCY	3 cut(s)	844, 1197, 1397
PvuII	CAGCTG	1 cut(s)	1481
RsaI	GTAC	4 cut(s)	542, 1260, 1355, 1490
RsaNI	GTAC	4 cut(s)	541, 1259, 1354, 1489
RseI	CAYNNNNRTG	1 cut(s)	435
SaqAI	TTAA	5 cut(s)	321, 666, 807, 900, 1158
SatI	GCNGC	2 cut(s)	501, 735
Sau3AI	GATC	9 cut(s)	92, 491, 688, 844, 913, 1197, 1331, 1397, 1417
SchI	GAGTC	4 cut(s)	203, 520, 550, 1272
ScrFI	CCNGG	9 cut(s)	206, 224, 248, 379, 548, 1065, 1242, 1248, 1310
SduI	GDGCHC	3 cut(s)	189, 247, 270
SfaNI	GCATC	4 cut(s)	126, 862, 955, 1333
SinI	GGWCC	6 cut(s)	10, 37, 390, 1018, 1026, 1077
SmiMI	CAYNNNNRTG	1 cut(s)	435
SmlI	CTYRAG	2 cut(s)	1223, 1281
SmoI	CTYRAG	2 cut(s)	1223, 1281
Sse9I	AATT	7 cut(s)	504, 756, 884, 936, 992, 1187, 1596
SsiI	CCGC	7 cut(s)	82, 163, 183, 478, 735, 1306, 1379
SspMI	CTAG	1 cut(s)	519
StyD4I	CCNGG	9 cut(s)	204, 222, 246, 377, 546, 1063, 1240, 1246, 1308
StyI	CCWWGG	2 cut(s)	6, 1021
TaaI	ACNGT	3 cut(s)	434, 676, 730
TaiI	ACGT	4 cut(s)	823, 1113, 1440, 1580
TaqI	TCGA	4 cut(s)	48, 91, 586, 720
TasI	AATT	7 cut(s)	504, 756, 884, 936, 992, 1187, 1596
TauI	GCSGC	1 cut(s)	737
TfiI	GAWTC	5 cut(s)	43, 88, 231, 722, 1049
Tru1I	TTAA	5 cut(s)	321, 666, 807, 900, 1158
Tru9I	TTAA	5 cut(s)	321, 666, 807, 900, 1158
TscAI	CASTG	2 cut(s)	622, 681
TseFI	GTSAC	1 cut(s)	553
TseI	GCWGC	1 cut(s)	500
Tsp45I	GTSAC	1 cut(s)	553
TspDTI	ATGAA	7 cut(s)	663, 737, 761, 839, 940, 1148, 1315
TspGWI	ACGGA	3 cut(s)	44, 91, 184
TspRI	CASTG	2 cut(s)	622, 681
Van91I	CCANNNNNTGG	2 cut(s)	1064, 1241
VpaK11BI	GGWCC	6 cut(s)	10, 37, 390, 1018, 1026, 1077
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1109, 1238
XmnI	GAANNNNTTC	2 cut(s)	885, 1429
XspI	CTAG	1 cut(s)	519
Zsp2I	ATGCAT	1 cut(s)	1294

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.