MD14G1143600.v1.1
MYB Family

Trihelix transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006309
Physical Location & Seq
Reverse (-)
23623095 .. 23627528
4434 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1143600.v1.1.491

Sequence Viewer

Length: 2184 bp
ATGCAGCAACCAGCAGCTGGAGGAGCTGGAGGGTCCCAAACCCATTACGGAGGAGCTGGAGAGATGAGTGCTGCTGAAGCTGCGGCGGCGGAGCAAGGCCAGCTGGTGGTGGAAGCGGCGTCCCCAATCAGCAGCAGGCCGCCAGCTTCCGCGGCGGTGAATTTGGACGAGCTCATGACTCTGTCCGGTGCGGCGGCTGCAGCAGAAGATGCTCTCGCGGATCGAGGTGGCGGTGGTGGGTCCACCGGGAACAGATGGCCGCGTCAGGAGACCCTGGCGCTTCTCAAAATCAGGTCCGATATGGATGTGGCCTTCCGTGACGCGACCCTCAAAGGCCCCTTGTGGGAAGATGTCTCCAGGAAACTGGCGGAATTGGGGTACCGAAGAAATGCCAAGAAATGCAAGGAGAAATTCGAAAACGTTCACAAATATTACAAGCGGACAAAAGAAGGCCGCGCGGGACGCCAAGACGGCAAAAGCTACAAGTTTTTCAGCGAGCTGGAGGCTCTACATGGCACCGCCGCCACCGCCGCAGCTACGACAGCCAACGTGTCAGCCTCTCCGGCAGTGCACGCCGCGTCTCCGAATCCGGTTTCTGTCGGGCTCGGATCTGTCAGTAACCCCATGCCCATCTCATCGTTCAGAATGTCACAGCAACAACAACCACCTTCCGCCACTGCAATTCCAATAATACCCTCGTCCCAACCTACAGCCACTCCAACGGACTTTAACTTCTCCTCCAACAGCTCGTCGACTTCCCCGGGAACTGACGACGATGACGACGATGATGACGCGGAAGGAGAGCGCTGGAGTCGGAAGCGAAAAAAGGGGAGTGCTAGTACTAGTACCGGAATTAGTGGCGGCGGCAGTACCCGAAAAATGATGGACTTTTTCGAGGTTCTGATGAAGCAAGTGATGCAAAAGCAAGAGAGCATGCAGCAGAGGTTTCTAGAAGTGATAGAGAAGAGGGAGCAGGACAGGACTATCAGGGAGGAGGCGTGGAAACGTCAGGAGATGGCCCGTTTAACTCGCGAGCAGGAGCTCATGAGTCAAGAGCGGGCCATCTCTGCTTCAAGAGATGCCGCCATTATCTCTTTCCTGCAGAAAATTACTGGTCAGACTATTCAGTTGCCTCCGCCGGTCAATGTCCACGCCGCTCCCCCACCACCTGTGCCTCCGTCGGTGTCGGTTGTCACGCCTTTACCACAGACAACGCCACAGCCGACGACACAGACTTATCAAACACCGCAACAGCAACAACCTCAGCCACCGCAACAAATGCAACAAGTTCGTCATGCTCAGCAGACTCAAGCTGTTCAACCGGTGATGGCTGTACCGGAACAGCAGGTACCTCCGACAGCGCAGGAGAATATTGGTAGCGGTGGAGGAGGAGGGAGCTCCGAGCCTGCATCATCTTCGAGATGGCCCAAGGCAGAAGTACTGGCGCTGATAAAGTTGAGGAGCGGGCTTGAGTCGAGGTACCAAGATGCAGGGCCGAAAGGCCCACTTTGGGAGGAAATCTCCGCCGGCATGGGGCGGATGGGGTACAATAGGAGTTGCAAGAGGTGCAAGGAGAAATGGGAGAACATCAACAAGTACTTCAAGAAGGTGAAGGAGAGCAACAAGAAGCGGCCCGAGGATGCGAAAACGTGTCCATATTTTCACGAACTAGATGCCCTTTACCGGAAAAGGGTGCTTGGCGGTGGGTCTACCGGCGGCAGCAGCAGCTCATTGGGAAATAGGCTAGAACAACAACCGCAACAACCTGAACAGGAGCAAGTAGTGGCCGGATCAGAAAACAAGAATGCAGATGATAGCACAGATGTGGAAACAAATTTGAGAGCTAACCTCTTTGGAGAGGGAACAGAAGAGGCTGCCAAGAAGCCAGAAGACATTGTGAAGGAGTTGATGGAGGTGCATGATCACCAACAAGTCCTCCAACAAGGGCACCCACAACATTTTGGAGTAGATGACTGCAATAGGGTGGAGGAAGCGGATAGCGATAATCTTATGGATCAAGAGGAGGAGGACATGGAAGACGAGGACATGGACGAGGAAGACGATGAAGAAACGGAACAAGAGAGGAAGATGGCTTACAAGATAGAGTTTCAGAAGCAGAATACAGGCCCTTCGTCCAACGGCGGAGGCAACGGTGCACCTTCTTTCCTCGCCCTGGTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0000904 GO:0001101 GO:0001558 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006275 GO:0006351 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008156 GO:0008361 GO:0009058 GO:0009059 GO:0009414 GO:0009415 GO:0009605 GO:0009628 GO:0009653 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010026 GO:0010035 GO:0010090 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010948 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030154 GO:0030308 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031668 GO:0032502 GO:0032535 GO:0032774 GO:0032875 GO:0032876 GO:0032989 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0040008 GO:0042221 GO:0042631 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045786 GO:0045892 GO:0045926 GO:0045934 GO:0046483 GO:0048468 GO:0048519 GO:0048523 GO:0048580 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051128 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0090066 GO:0090304 GO:0090329 GO:0090558 GO:0090626 GO:0097159 GO:0097659 GO:0104004 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000026 GO:2000037 GO:2000038 GO:2000104 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-bind_4

Protein Analysis

728

Amino Acids

79.13

Weight (kDa)

5.23

Isoelectric Point (pI)

65.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_4 PF13837 84 - 170 2.5e-23 Myb/SANT-like DNA-binding domain
Myb_DNA-bind_4 PF13837 473 - 560 4.5e-25 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1336
Acc65I GGTACC 3 cut(s) 378, 1348, 1479
AccB1I GGYRCC 5 cut(s) 378, 515, 1348, 1479, 1947
AccB7I CCANNNNNTGG 2 cut(s) 17, 106
AccBSI CCGCTC 3 cut(s) 1057, 1157, 1464
AccI GTMKAC 2 cut(s) 752, 1709
AccII CGCG 9 cut(s) 152, 218, 262, 323, 456, 458, 578, 794, 1032
AclI AACGTT 1 cut(s) 420
AclWI GGATC 4 cut(s) 228, 616, 1798, 2022
AcoI YGGCCR 2 cut(s) 257, 1785
AcsI RAATTY 3 cut(s) 160, 410, 1834
AcuI CTGAAG 1 cut(s) 96
AcyI GRCGYC 2 cut(s) 119, 463
AfeI AGCGCT 1 cut(s) 806
AfiI CCNNNNNNNGG 8 cut(s) 17, 106, 343, 1510, 1533, 1683, 1690, 2173
AflIII ACRYGT 2 cut(s) 549, 1649
AgeI ACCGGT 1 cut(s) 1321
AgsI TTSAA 3 cut(s) 1074, 1319, 1603
AhlI ACTAGT 1 cut(s) 842
AjnI CCWGG 3 cut(s) 273, 356, 2172
AleI CACNNNNGTG 1 cut(s) 1823
Alw21I GWGCWC 5 cut(s) 174, 573, 1044, 1400, 2158
Alw26I GTCTC 3 cut(s) 263, 358, 585
Alw44I GTGCAC 2 cut(s) 569, 2154
AlwI GGATC 4 cut(s) 228, 616, 1798, 2022
AlwNI CAGNNNCTG 1 cut(s) 17
Ama87I CYCGRG 2 cut(s) 760, 1634
Aor51HI AGCGCT 1 cut(s) 806
ApaLI GTGCAC 2 cut(s) 569, 2154
ApoI RAATTY 3 cut(s) 160, 410, 1834
AsiGI ACCGGT 1 cut(s) 1321
Asp700I GAANNNNTTC 1 cut(s) 420
Asp718I GGTACC 3 cut(s) 378, 1348, 1479
AspLEI GCGC 5 cut(s) 280, 458, 807, 1363, 1447
AsuC2I CCSGG 3 cut(s) 247, 761, 762
AsuHPI GGTGA 4 cut(s) 169, 1336, 1621, 1916
AsuII TTCGAA 1 cut(s) 414
AvaI CYCGRG 2 cut(s) 760, 1634
AvaII GGWCC 3 cut(s) 33, 240, 294
BaeGI GKGCMC 3 cut(s) 573, 1950, 2158
BanI GGYRCC 5 cut(s) 378, 515, 1348, 1479, 1947
BanII GRGCYC 4 cut(s) 174, 606, 1044, 1400
BarI GAAGNNNNNNTAC 2 cut(s) 2078, 2110
BbsI GAAGAC 3 cut(s) 1896, 2043, 2064
Bbv12I GWGCWC 5 cut(s) 174, 573, 1044, 1400, 2158
BbvCI CCTCAGC 1 cut(s) 1263
BceAI ACGGC 2 cut(s) 487, 2155
BcgI CGANNNNNNTGC 6 cut(s) 1271, 1305, 1398, 1432, 1655, 1689
BciT130I CCWGG 3 cut(s) 275, 358, 2174
BclI TGATCA 1 cut(s) 1921
BcnI CCSGG 3 cut(s) 247, 761, 762
BcoDI GTCTC 3 cut(s) 263, 358, 585
BcuI ACTAGT 1 cut(s) 842
BfaI CTAG 5 cut(s) 837, 843, 950, 1670, 1745
BfmI CTRYAG 3 cut(s) 198, 708, 1100
BfoI RGCGCY 3 cut(s) 281, 808, 1448
BfuAI ACCTGC 1 cut(s) 1336
BglI GCCNNNNNGGC 2 cut(s) 471, 563
BlpI GCTNAGC 1 cut(s) 1299
BmcAI AGTACT 3 cut(s) 841, 1440, 1598
Bme1390I CCNGG 6 cut(s) 247, 275, 358, 761, 762, 2174
Bme18I GGWCC 3 cut(s) 33, 240, 294
BmeT110I CYCGRG 2 cut(s) 760, 1634
BmiI GGNNCC 9 cut(s) 34, 35, 241, 337, 380, 517, 1350, 1481, 1949
BmrFI CCNGG 6 cut(s) 247, 275, 358, 761, 762, 2174
BmsI GCATC 7 cut(s) 199, 906, 1069, 1418, 1477, 1630, 1663
BpiI GAAGAC 3 cut(s) 1896, 2043, 2064
BplI GAGNNNNNCTC 4 cut(s) 1505, 1537, 1833, 1865
BpmI CTGGAG 6 cut(s) 39, 48, 78, 340, 521, 829
Bpu10I CCTNAGC 1 cut(s) 1263
Bpu1102I GCTNAGC 1 cut(s) 1299
Bpu14I TTCGAA 1 cut(s) 414
BpuEI CTTGAG 2 cut(s) 1293, 1490
BpuMI CCSGG 3 cut(s) 247, 761, 762
BsaHI GRCGYC 2 cut(s) 119, 463
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 7 cut(s) 150, 273, 759, 760, 1428, 1635, 2172
BsaWI WCCGGW 6 cut(s) 185, 589, 848, 1321, 1336, 1683
Bsc4I CCNNNNNNNGG 8 cut(s) 17, 106, 343, 1510, 1533, 1683, 1690, 2173
Bse118I RCCGGY 4 cut(s) 1138, 1321, 1526, 1712
Bse1I ACTGG 3 cut(s) 369, 1117, 1446
BseBI CCWGG 3 cut(s) 275, 358, 2174
BseDI CCNNGG 7 cut(s) 150, 273, 759, 760, 1428, 1635, 2172
BseGI GGATG 3 cut(s) 310, 1545, 1645
BseLI CCNNNNNNNGG 8 cut(s) 17, 106, 343, 1510, 1533, 1683, 1690, 2173
BseMII CTCAG 2 cut(s) 1277, 1313
BseNI ACTGG 3 cut(s) 369, 1117, 1446
BseRI GAGGAG 9 cut(s) 36, 66, 727, 1007, 1401, 1404, 1474, 2036, 2039
BseSI GKGCMC 3 cut(s) 573, 1950, 2158
Bsh1236I CGCG 9 cut(s) 152, 218, 262, 323, 456, 458, 578, 794, 1032
BshNI GGYRCC 5 cut(s) 378, 515, 1348, 1479, 1947
BshTI ACCGGT 1 cut(s) 1321
BsiHKAI GWGCWC 5 cut(s) 174, 573, 1044, 1400, 2158
BsiHKCI CYCGRG 2 cut(s) 760, 1634
BslFI GGGAC 4 cut(s) 19, 106, 474, 685
BslI CCNNNNNNNGG 8 cut(s) 17, 106, 343, 1510, 1533, 1683, 1690, 2173
BsmAI GTCTC 3 cut(s) 263, 358, 585
BsmBI CGTCTC 1 cut(s) 585
BsmFI GGGAC 4 cut(s) 19, 106, 474, 685
BsmI GAATGC 1 cut(s) 1810
Bso31I GGTCTC 1 cut(s) 263
BsoBI CYCGRG 2 cut(s) 760, 1634
Bsp119I TTCGAA 1 cut(s) 414
Bsp1286I GDGCHC 7 cut(s) 174, 573, 606, 1044, 1400, 1950, 2158
Bsp143I GATC 5 cut(s) 220, 608, 1790, 1921, 2014
Bsp1720I GCTNAGC 1 cut(s) 1299
Bsp68I TCGCGA 1 cut(s) 1032
BspCNI CTCAG 2 cut(s) 1276, 1312
BspFNI CGCG 9 cut(s) 152, 218, 262, 323, 456, 458, 578, 794, 1032
BspHI TCATGA 2 cut(s) 174, 1044
BspLI GGNNCC 9 cut(s) 34, 35, 241, 337, 380, 517, 1350, 1481, 1949
BspMAI CTGCAG 2 cut(s) 202, 1104
BspMI ACCTGC 1 cut(s) 1336
BspPI GGATC 4 cut(s) 228, 616, 1798, 2022
BspT104I TTCGAA 1 cut(s) 414
BspT107I GGYRCC 5 cut(s) 378, 515, 1348, 1479, 1947
BspTNI GGTCTC 1 cut(s) 263
BsrBI CCGCTC 3 cut(s) 1057, 1157, 1464
BsrFI RCCGGY 4 cut(s) 1138, 1321, 1526, 1712
BsrI ACTGG 3 cut(s) 369, 1117, 1446
BssAI RCCGGY 4 cut(s) 1138, 1321, 1526, 1712
BssECI CCNNGG 7 cut(s) 150, 273, 759, 760, 1428, 1635, 2172
BssMI GATC 5 cut(s) 220, 608, 1790, 1921, 2014
BssNI GRCGYC 2 cut(s) 119, 463
BssT1I CCWWGG 1 cut(s) 1428
Bst2UI CCWGG 3 cut(s) 275, 358, 2174
Bst4CI ACNGT 1 cut(s) 2153
Bst6I CTCTTC 2 cut(s) 959, 1863
BstACI GRCGYC 2 cut(s) 119, 463
BstAPI GCANNNNNTGC 4 cut(s) 209, 916, 1279, 1566
BstBI TTCGAA 1 cut(s) 414
BstDEI CTNAG 2 cut(s) 1263, 1299
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 3 cut(s) 310, 1545, 1645
BstFNI CGCG 9 cut(s) 152, 218, 262, 323, 456, 458, 578, 794, 1032
BstH2I RGCGCY 3 cut(s) 281, 808, 1448
BstHHI GCGC 5 cut(s) 280, 458, 807, 1363, 1447
BstKTI GATC 5 cut(s) 223, 611, 1793, 1924, 2017
BstMAI GTCTC 3 cut(s) 263, 358, 585
BstMBI GATC 5 cut(s) 220, 608, 1790, 1921, 2014
BstNI CCWGG 3 cut(s) 275, 358, 2174
BstNSI RCATGY 1 cut(s) 937
BstSCI CCNGG 6 cut(s) 245, 273, 356, 759, 760, 2172
BstSFI CTRYAG 3 cut(s) 198, 708, 1100
BstSLI GKGCMC 3 cut(s) 573, 1950, 2158
BstUI CGCG 9 cut(s) 152, 218, 262, 323, 456, 458, 578, 794, 1032
BstV2I GAAGAC 3 cut(s) 1896, 2043, 2064
BstX2I RGATCY 1 cut(s) 608
BstXI CCANNNNNNTGG 1 cut(s) 364
BstYI RGATCY 1 cut(s) 608
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 3 cut(s) 310, 1545, 1645
BtsI GCAGTG 2 cut(s) 573, 675
BtsIMutI CAGTG 2 cut(s) 573, 675
BtuMI TCGCGA 1 cut(s) 1032
BveI ACCTGC 1 cut(s) 1336
CaiI CAGNNNCTG 1 cut(s) 17
CciI TCATGA 2 cut(s) 174, 1044
CfoI GCGC 5 cut(s) 280, 458, 807, 1363, 1447
Cfr10I RCCGGY 4 cut(s) 1138, 1321, 1526, 1712
Cfr42I CCGCGG 1 cut(s) 153
Cfr9I CCCGGG 1 cut(s) 760
CseI GACGC 6 cut(s) 108, 251, 329, 471, 567, 800
CspAI ACCGGT 1 cut(s) 1321
DdeI CTNAG 2 cut(s) 1263, 1299
DpnI GATC 5 cut(s) 222, 610, 1792, 1923, 2016
DpnII GATC 5 cut(s) 220, 608, 1790, 1921, 2014
EaeI YGGCCR 2 cut(s) 257, 1785
Eam1104I CTCTTC 2 cut(s) 959, 1863
EarI CTCTTC 2 cut(s) 959, 1863
EciI GGCGGA 7 cut(s) 104, 383, 661, 1125, 1513, 1552, 2157
Ecl136II GAGCTC 3 cut(s) 172, 1042, 1398
Eco130I CCWWGG 1 cut(s) 1428
Eco24I GRGCYC 4 cut(s) 174, 606, 1044, 1400
Eco31I GGTCTC 1 cut(s) 263
Eco47I GGWCC 3 cut(s) 33, 240, 294
Eco47III AGCGCT 1 cut(s) 806
Eco53kI GAGCTC 3 cut(s) 172, 1042, 1398
Eco57I CTGAAG 1 cut(s) 96
Eco88I CYCGRG 2 cut(s) 760, 1634
EcoICRI GAGCTC 3 cut(s) 172, 1042, 1398
EcoO109I RGGNCCY 3 cut(s) 33, 335, 2126
EcoRII CCWGG 3 cut(s) 273, 356, 2172
EcoT14I CCWWGG 1 cut(s) 1428
EcoT38I GRGCYC 4 cut(s) 174, 606, 1044, 1400
ErhI CCWWGG 1 cut(s) 1428
Esp3I CGTCTC 1 cut(s) 585
FalI AAGNNNNNCTT 2 cut(s) 1491, 1523
FaqI GGGAC 4 cut(s) 19, 106, 474, 685
FauI CCCGC 3 cut(s) 451, 1050, 1457
FbaI TGATCA 1 cut(s) 1921
FblI GTMKAC 2 cut(s) 752, 1709
FokI GGATG 3 cut(s) 317, 1552, 1652
FriOI GRGCYC 4 cut(s) 174, 606, 1044, 1400
FspBI CTAG 5 cut(s) 837, 843, 950, 1670, 1745
GlaI GCGC 5 cut(s) 279, 457, 806, 1362, 1446
GsuI CTGGAG 6 cut(s) 39, 48, 78, 340, 521, 829
HaeII RGCGCY 3 cut(s) 281, 808, 1448
HgaI GACGC 6 cut(s) 108, 251, 329, 471, 567, 800
HhaI GCGC 5 cut(s) 280, 458, 807, 1363, 1447
Hin1I GRCGYC 2 cut(s) 119, 463
Hin6I GCGC 5 cut(s) 278, 456, 805, 1361, 1445
HinP1I GCGC 5 cut(s) 278, 456, 805, 1361, 1445
HincII GTYRAC 1 cut(s) 753
HindII GTYRAC 1 cut(s) 753
HinfI GANTC 6 cut(s) 178, 586, 811, 1048, 1306, 1472
HphI GGTGA 4 cut(s) 169, 1336, 1621, 1916
Hpy166II GTNNAC 7 cut(s) 243, 424, 571, 753, 1150, 1710, 2156
Hpy8I GTNNAC 7 cut(s) 243, 424, 571, 753, 1150, 1710, 2156
Hpy99I CGWCG 5 cut(s) 754, 776, 785, 1183, 1228
HpyAV CCTTC 9 cut(s) 322, 443, 678, 791, 1600, 1606, 1894, 2139, 2169
HpyCH4III ACNGT 1 cut(s) 2153
HpyCH4IV ACGT 4 cut(s) 420, 549, 1006, 1649
HpyF3I CTNAG 2 cut(s) 1263, 1299
HpySE526I ACGT 4 cut(s) 420, 549, 1006, 1649
Hsp92I GRCGYC 2 cut(s) 119, 463
HspAI GCGC 5 cut(s) 278, 456, 805, 1361, 1445
KflI GGGWCCC 1 cut(s) 33
KpnI GGTACC 3 cut(s) 382, 1352, 1483
KroI GCCGGC 1 cut(s) 1526
KroNI GCCGGC 1 cut(s) 1528
Ksp22I TGATCA 1 cut(s) 1921
KspI CCGCGG 1 cut(s) 153
Kzo9I GATC 5 cut(s) 220, 608, 1790, 1921, 2014
LweI GCATC 7 cut(s) 199, 906, 1069, 1418, 1477, 1630, 1663
MaeI CTAG 5 cut(s) 837, 843, 950, 1670, 1745
MaeII ACGT 4 cut(s) 420, 549, 1006, 1649
MaeIII GTNAC 4 cut(s) 317, 617, 648, 1192
MalI GATC 5 cut(s) 222, 610, 1792, 1923, 2016
MbiI CCGCTC 3 cut(s) 1057, 1157, 1464
MboI GATC 5 cut(s) 220, 608, 1790, 1921, 2014
MflI RGATCY 1 cut(s) 608
MhlI GDGCHC 7 cut(s) 174, 573, 606, 1044, 1400, 1950, 2158
MluCI AATT 7 cut(s) 160, 371, 410, 681, 852, 1107, 1834
MlyI GAGTC 5 cut(s) 172, 820, 1057, 1300, 1481
MmeI TCCRAC 6 cut(s) 743, 765, 794, 1379, 1963, 2160
MroNI GCCGGC 1 cut(s) 1526
MroXI GAANNNNTTC 1 cut(s) 420
MseI TTAA 2 cut(s) 729, 1025
MslI CAYNNNNRTG 1 cut(s) 1823
MspA1I CMGCKG 3 cut(s) 17, 103, 152
MspR9I CCNGG 6 cut(s) 247, 275, 358, 761, 762, 2174
Mva1269I GAATGC 1 cut(s) 1810
MvaI CCWGG 3 cut(s) 275, 358, 2174
MvnI CGCG 9 cut(s) 152, 218, 262, 323, 456, 458, 578, 794, 1032
NaeI GCCGGC 1 cut(s) 1528
NciI CCSGG 3 cut(s) 247, 761, 762
NdeII GATC 5 cut(s) 220, 608, 1790, 1921, 2014
NgoMIV GCCGGC 1 cut(s) 1526
NlaIV GGNNCC 9 cut(s) 34, 35, 241, 337, 380, 517, 1350, 1481, 1949
NmuCI GTSAC 3 cut(s) 317, 648, 1192
NruI TCGCGA 1 cut(s) 1032
NspI RCATGY 1 cut(s) 937
NspV TTCGAA 1 cut(s) 414
OliI CACNNNNGTG 1 cut(s) 1823
PaeI GCATGC 1 cut(s) 937
PagI TCATGA 2 cut(s) 174, 1044
PcsI WCGNNNNNNNCGW 4 cut(s) 777, 780, 1220, 2058
PctI GAATGC 1 cut(s) 1810
PdiI GCCGGC 1 cut(s) 1528
PdmI GAANNNNTTC 1 cut(s) 420
PfeI GAWTC 1 cut(s) 586
PflFI GACNNNGTC 1 cut(s) 181
PflMI CCANNNNNTGG 2 cut(s) 17, 106
PfoI TCCNGGA 1 cut(s) 356
PinAI ACCGGT 1 cut(s) 1321
PleI GAGTC 5 cut(s) 172, 819, 1056, 1300, 1480
PpsI GAGTC 5 cut(s) 172, 819, 1056, 1300, 1480
PpuMI RGGWCCY 1 cut(s) 33
Psp124BI GAGCTC 3 cut(s) 174, 1044, 1400
Psp1406I AACGTT 1 cut(s) 420
Psp5II RGGWCCY 1 cut(s) 33
Psp6I CCWGG 3 cut(s) 273, 356, 2172
PspGI CCWGG 3 cut(s) 273, 356, 2172
PspN4I GGNNCC 9 cut(s) 34, 35, 241, 337, 380, 517, 1350, 1481, 1949
PspPPI RGGWCCY 1 cut(s) 33
PsrI GAACNNNNNNTAC 2 cut(s) 1332, 1364
PstI CTGCAG 2 cut(s) 202, 1104
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 1 cut(s) 608
PsyI GACNNNGTC 1 cut(s) 181
PvuII CAGCTG 2 cut(s) 17, 103
RruI TCGCGA 1 cut(s) 1032
RseI CAYNNNNRTG 1 cut(s) 1823
SacI GAGCTC 3 cut(s) 174, 1044, 1400
SacII CCGCGG 1 cut(s) 153
SalI GTCGAC 1 cut(s) 751
SaqAI TTAA 2 cut(s) 729, 1025
Sau3AI GATC 5 cut(s) 220, 608, 1790, 1921, 2014
ScaI AGTACT 3 cut(s) 841, 1440, 1598
SchI GAGTC 5 cut(s) 172, 820, 1057, 1300, 1481
ScrFI CCNGG 6 cut(s) 247, 275, 358, 761, 762, 2174
SduI GDGCHC 7 cut(s) 174, 573, 606, 1044, 1400, 1950, 2158
SfaNI GCATC 7 cut(s) 199, 906, 1069, 1418, 1477, 1630, 1663
SfcI CTRYAG 3 cut(s) 198, 708, 1100
Sfr303I CCGCGG 1 cut(s) 153
SfuI TTCGAA 1 cut(s) 414
SgrBI CCGCGG 1 cut(s) 153
SinI GGWCC 3 cut(s) 33, 240, 294
SmaI CCCGGG 1 cut(s) 762
SmiMI CAYNNNNRTG 1 cut(s) 1823
SmlI CTYRAG 2 cut(s) 1308, 1469
SmoI CTYRAG 2 cut(s) 1308, 1469
SpeI ACTAGT 1 cut(s) 842
SphI GCATGC 1 cut(s) 937
Sse9I AATT 7 cut(s) 160, 371, 410, 681, 852, 1107, 1834
SspI AATATT 2 cut(s) 431, 1372
SspMI CTAG 5 cut(s) 837, 843, 950, 1670, 1745
SstI GAGCTC 3 cut(s) 174, 1044, 1400
StyD4I CCNGG 6 cut(s) 245, 273, 356, 759, 760, 2172
StyI CCWWGG 1 cut(s) 1428
TaaI ACNGT 1 cut(s) 2153
TaiI ACGT 4 cut(s) 423, 552, 1009, 1652
TaqI TCGA 6 cut(s) 223, 414, 752, 894, 1418, 1475
TasI AATT 7 cut(s) 160, 371, 410, 681, 852, 1107, 1834
TatI WGTACW 3 cut(s) 839, 1438, 1596
TfiI GAWTC 1 cut(s) 586
Tru1I TTAA 2 cut(s) 729, 1025
Tru9I TTAA 2 cut(s) 729, 1025
TscAI CASTG 2 cut(s) 573, 682
TseFI GTSAC 3 cut(s) 317, 648, 1192
Tsp45I GTSAC 3 cut(s) 317, 648, 1192
TspDTI ATGAA 2 cut(s) 920, 2079
TspGWI ACGGA 5 cut(s) 63, 305, 737, 1167, 2087
TspMI CCCGGG 1 cut(s) 760
TspRI CASTG 2 cut(s) 573, 682
Tth111I GACNNNGTC 1 cut(s) 181
Van91I CCANNNNNTGG 2 cut(s) 17, 106
VneI GTGCAC 2 cut(s) 569, 2154
VpaK11BI GGWCC 3 cut(s) 33, 240, 294
XapI RAATTY 3 cut(s) 160, 410, 1834
XbaI TCTAGA 1 cut(s) 949
XceI RCATGY 1 cut(s) 937
XmaI CCCGGG 1 cut(s) 760
XmiI GTMKAC 2 cut(s) 752, 1709
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 5 cut(s) 837, 843, 950, 1670, 1745
ZrmI AGTACT 3 cut(s) 841, 1440, 1598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.