Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0014431

Physical Location & Seq

Genomic Coordinates Forward (+)

24454255 .. 24456823

Gene Structure 2569 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1151300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 897 bp

ATGACTGCATCAACTTCTCACCATCCATTGGCTTTTGCCTTTGGCATTCTAGGCAACATTGTCTCATTCATCGTTTTTCTAGCTCCGCTGCCGACATTTTGGAGGGTGTATAAAAAGAAATCGACGGAGGGATTTCAATCAGTTCCATATGTGTTTGCACTATTCAGTGCAATGATATGGATATACTATGCATTCCTCAAGTCTCATGTGATCCTGCTCATCACCATCAACTCATTCGGTTGTGTCATAGAGACCATTTATATTGCAATTTACCTTACATATGCAACTAAGCATGCGAGGGTGTCTACTCTGAGGCTGCTTTTACTGGTGAACTTCGGGGGATTTTGCTTGATTCTTCTTCTGTCTCACTTCTTGTCACAAGGGCCGACCCGCGTCGAAGTTCTTGGATGGGTTTGTGTGACTTTCTCTGTCAGTGTCTTTGCAGCACCTTTAAGCGTCATGAGAATGGTTATCCGCACCAAGAGCGTGGAGTTCATGCCGTTTAATTTATCCTTCTTCCTCACCCTAAGTGCCGTTATGTGGCTCTGTTATGGCTTACTCCTCAAGGATCTCTACGTTGCAACCCCAAACATACTTGGTTTTTCCTTTGGGGTGGTTCAGATGGCCCTCTATGCAAAGTACAGGAACACCAAAACAATTGTGGAGGAAAAGCTACCAGAACACAAGGCTGATGTTGTGAAGCAAATCAAAATTTTAACTACTACTCCTGAGGTGGAGGTACAAGTCCAAGCACCTGTAACCTCGGATACCAATAGTACTGATGCTCATCAAAACTCTGAGCGTGGCACTGACCAATATGTGCATGCACAAACATGCCGTAACGAGAAGATCATCGAACCATCCATGCCAGGCCAACTGGTTACATGTGAAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0007275 GO:0008150 GO:0008515 GO:0008643 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010431 GO:0015144 GO:0015154 GO:0015157 GO:0015766 GO:0015770 GO:0015772 GO:0016020 GO:0016021 GO:0016043 GO:0021700 GO:0022414 GO:0022607 GO:0022857 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0034219 GO:0043933 GO:0044085 GO:0044425 GO:0044459 GO:0044464 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0051119 GO:0051179 GO:0051234 GO:0051259 GO:0051260 GO:0055085 GO:0061458 GO:0065003 GO:0071695 GO:0071702 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MtN3_slv

Protein Analysis

299

Amino Acids

33.49

Weight (kDa)

8.82

Isoelectric Point (pI)

32.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MtN3_slv	PF03083	13 - 98	3.5e-26	Sugar efflux transporter for intercellular exchange
MtN3_slv	PF03083	134 - 218	4.4e-28	Sugar efflux transporter for intercellular exchange

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0014431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48740 AT5G23660
fragaria_vesca	FvH4_5g08970
malus_domestica	MD06G1136500.v1.1 MD14G1151300.v1.1
prunus_persica	Prupe.5G146400_v2.0.a1
pyrus_communis	pycom14g12600 pycom14g12610
rosa_chinensis	RchiOBHm_Chr7g0189331
rosa_laevigata	RLG00000004618
rosa_multiflora	Rmu_sc0001282.1_g000001
rosa_roxburghii	Rroxscaffold_3G00265530
rosa_rugosa	Rorug06G0497200
rosa_samantha	Rh7AG102200 Rh7BG104800 Rh7DG103800
rosa_wichuraiana	Rw7G008830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	28
AccI	GTMKAC	1 cut(s)	305
AccII	CGCG	1 cut(s)	393
AciI	CCGC	3 cut(s)	86, 391, 475
AclWI	GGATC	2 cut(s)	205, 576
AcsI	RAATTY	1 cut(s)	711
AfaI	GTAC	3 cut(s)	641, 741, 778
AfiI	CCNNNNNNNGG	2 cut(s)	28, 540
AflIII	ACRYGT	1 cut(s)	884
AgsI	TTSAA	1 cut(s)	137
AjnI	CCWGG	1 cut(s)	868
AluBI	AGCT	2 cut(s)	83, 673
AluI	AGCT	2 cut(s)	83, 673
Alw26I	GTCTC	4 cut(s)	67, 207, 245, 369
AlwI	GGATC	2 cut(s)	205, 576
AoxI	GGCC	3 cut(s)	383, 624, 871
ApeKI	GCWGC	3 cut(s)	88, 316, 443
ApoI	RAATTY	1 cut(s)	711
AspS9I	GGNCC	2 cut(s)	383, 625
AsuHPI	GGTGA	4 cut(s)	11, 214, 340, 514
AxyI	CCTNAGG	1 cut(s)	729
BbvI	GCAGC	3 cut(s)	75, 303, 455
BccI	CCATC	5 cut(s)	30, 233, 402, 616, 868
BceAI	ACGGC	3 cut(s)	484, 518, 822
BciT130I	CCWGG	1 cut(s)	870
BciVI	GTATCC	1 cut(s)	760
BcoDI	GTCTC	4 cut(s)	67, 207, 245, 369
BfaI	CTAG	2 cut(s)	50, 80
BfuI	GTATCC	1 cut(s)	760
BisI	GCNGC	3 cut(s)	89, 317, 444
BlsI	GCNGC	3 cut(s)	90, 318, 445
BmcAI	AGTACT	1 cut(s)	778
Bme1390I	CCNGG	1 cut(s)	870
BmgT120I	GGNCC	2 cut(s)	383, 625
BmrFI	CCNGG	1 cut(s)	870
BmsI	GCATC	2 cut(s)	17, 772
BoxI	GACNNNNGTC	1 cut(s)	392
BpuEI	CTTGAG	2 cut(s)	182, 548
BsaBI	GATNNNNATC	2 cut(s)	136, 786
BsaI	GGTCTC	1 cut(s)	245
BsaJI	CCNNGG	1 cut(s)	762
BsaXI	ACNNNNNCTCC	2 cut(s)	709, 739
Bsc4I	CCNNNNNNNGG	2 cut(s)	28, 540
Bse1I	ACTGG	2 cut(s)	330, 882
Bse21I	CCTNAGG	1 cut(s)	729
Bse3DI	GCAATG	1 cut(s)	177
Bse8I	GATNNNNATC	2 cut(s)	136, 786
BseBI	CCWGG	1 cut(s)	870
BseDI	CCNNGG	1 cut(s)	762
BseGI	GGATG	3 cut(s)	22, 413, 860
BseJI	GATNNNNATC	2 cut(s)	136, 786
BseLI	CCNNNNNNNGG	2 cut(s)	28, 540
BseMI	GCAATG	1 cut(s)	177
BseMII	CTCAG	3 cut(s)	302, 720, 789
BseNI	ACTGG	2 cut(s)	330, 882
BseRI	GAGGAG	1 cut(s)	551
BseXI	GCAGC	3 cut(s)	75, 303, 455
Bsh1236I	CGCG	1 cut(s)	393
BshFI	GGCC	3 cut(s)	385, 626, 873
BslI	CCNNNNNNNGG	2 cut(s)	28, 540
BsmAI	GTCTC	4 cut(s)	67, 207, 245, 369
BsmI	GAATGC	2 cut(s)	45, 191
BsnI	GGCC	3 cut(s)	385, 626, 873
Bso31I	GGTCTC	1 cut(s)	245
Bsp143I	GATC	3 cut(s)	210, 568, 849
BspACI	CCGC	3 cut(s)	86, 391, 475
BspANI	GGCC	3 cut(s)	385, 626, 873
BspCNI	CTCAG	3 cut(s)	303, 721, 790
BspFNI	CGCG	1 cut(s)	393
BspHI	TCATGA	1 cut(s)	459
BspPI	GGATC	2 cut(s)	205, 576
BspTNI	GGTCTC	1 cut(s)	245
BsrDI	GCAATG	1 cut(s)	177
BsrI	ACTGG	2 cut(s)	330, 882
BssECI	CCNNGG	1 cut(s)	762
BssMI	GATC	3 cut(s)	210, 568, 849
Bst2UI	CCWGG	1 cut(s)	870
BstC8I	GCNNGC	2 cut(s)	294, 825
BstDEI	CTNAG	5 cut(s)	288, 311, 527, 729, 798
BstF5I	GGATG	3 cut(s)	22, 413, 860
BstFNI	CGCG	1 cut(s)	393
BstKTI	GATC	3 cut(s)	213, 571, 852
BstMAI	GTCTC	4 cut(s)	67, 207, 245, 369
BstMBI	GATC	3 cut(s)	210, 568, 849
BstMWI	GCNNNNNNNGC	3 cut(s)	51, 483, 632
BstNI	CCWGG	1 cut(s)	870
BstNSI	RCATGY	4 cut(s)	296, 827, 837, 888
BstPAI	GACNNNNGTC	1 cut(s)	392
BstSCI	CCNGG	1 cut(s)	868
BstUI	CGCG	1 cut(s)	393
BstV1I	GCAGC	3 cut(s)	75, 303, 455
BstX2I	RGATCY	1 cut(s)	568
BstXI	CCANNNNNNTGG	1 cut(s)	487
BstYI	RGATCY	1 cut(s)	568
Bsu36I	CCTNAGG	1 cut(s)	729
BsuI	GTATCC	1 cut(s)	760
BsuRI	GGCC	3 cut(s)	385, 626, 873
BtsCI	GGATG	3 cut(s)	22, 413, 860
BtsIMutI	CAGTG	3 cut(s)	172, 439, 807
Cac8I	GCNNGC	2 cut(s)	294, 825
CciI	TCATGA	1 cut(s)	459
Cfr13I	GGNCC	2 cut(s)	383, 625
CseI	GACGC	2 cut(s)	382, 445
Csp6I	GTAC	3 cut(s)	640, 740, 777
CviAII	CATG	8 cut(s)	206, 293, 460, 496, 824, 834, 865, 885
CviQI	GTAC	3 cut(s)	640, 740, 777
DdeI	CTNAG	5 cut(s)	288, 311, 527, 729, 798
DpnI	GATC	3 cut(s)	212, 570, 851
DpnII	GATC	3 cut(s)	210, 568, 849
Eco31I	GGTCTC	1 cut(s)	245
Eco81I	CCTNAGG	1 cut(s)	729
EcoRII	CCWGG	1 cut(s)	868
EcoT22I	ATGCAT	1 cut(s)	193
FaeI	CATG	8 cut(s)	209, 296, 463, 499, 827, 837, 868, 888
FatI	CATG	8 cut(s)	205, 292, 459, 495, 823, 833, 864, 884
FauI	CCCGC	1 cut(s)	398
FauNDI	CATATG	2 cut(s)	148, 280
FblI	GTMKAC	1 cut(s)	305
Fnu4HI	GCNGC	3 cut(s)	89, 317, 444
FokI	GGATG	3 cut(s)	9, 420, 847
Fsp4HI	GCNGC	3 cut(s)	89, 317, 444
FspBI	CTAG	2 cut(s)	50, 80
GluI	GCNGC	3 cut(s)	89, 317, 444
HaeIII	GGCC	3 cut(s)	385, 626, 873
HgaI	GACGC	2 cut(s)	382, 445
Hin1II	CATG	8 cut(s)	209, 296, 463, 499, 827, 837, 868, 888
HinfI	GANTC	1 cut(s)	352
HphI	GGTGA	4 cut(s)	11, 214, 340, 514
Hpy166II	GTNNAC	2 cut(s)	306, 331
Hpy188I	TCNGA	4 cut(s)	312, 621, 766, 799
Hpy188III	TCNNGA	2 cut(s)	460, 728
Hpy8I	GTNNAC	2 cut(s)	306, 331
Hpy99I	CGWCG	2 cut(s)	127, 398
HpyAV	CCTTC	1 cut(s)	523
HpyCH4IV	ACGT	1 cut(s)	576
HpyF10VI	GCNNNNNNNGC	3 cut(s)	51, 483, 632
HpyF3I	CTNAG	5 cut(s)	288, 311, 527, 729, 798
HpySE526I	ACGT	1 cut(s)	576
Hsp92II	CATG	8 cut(s)	209, 296, 463, 499, 827, 837, 868, 888
Kzo9I	GATC	3 cut(s)	210, 568, 849
LmnI	GCTCC	1 cut(s)	88
LpnPI	CCDG	9 cut(s)	227, 311, 628, 690, 741, 768, 855, 863, 882
Lsp1109I	GCAGC	3 cut(s)	75, 303, 455
LweI	GCATC	2 cut(s)	17, 772
MaeI	CTAG	2 cut(s)	50, 80
MaeII	ACGT	1 cut(s)	576
MaeIII	GTNAC	5 cut(s)	375, 418, 757, 839, 880
MalI	GATC	3 cut(s)	212, 570, 851
MboI	GATC	3 cut(s)	210, 568, 849
MboII	GAAGA	4 cut(s)	347, 350, 508, 859
MfeI	CAATTG	1 cut(s)	657
MflI	RGATCY	1 cut(s)	568
MluCI	AATT	4 cut(s)	267, 505, 657, 711
Mph1103I	ATGCAT	1 cut(s)	193
MseI	TTAA	3 cut(s)	452, 504, 716
MslI	CAYNNNNRTG	2 cut(s)	464, 832
MspA1I	CMGCKG	1 cut(s)	88
MspR9I	CCNGG	1 cut(s)	870
MunI	CAATTG	1 cut(s)	657
Mva1269I	GAATGC	2 cut(s)	45, 191
MvaI	CCWGG	1 cut(s)	870
MvnI	CGCG	1 cut(s)	393
MwoI	GCNNNNNNNGC	3 cut(s)	51, 483, 632
NdeI	CATATG	2 cut(s)	148, 280
NdeII	GATC	3 cut(s)	210, 568, 849
NlaIII	CATG	8 cut(s)	209, 296, 463, 499, 827, 837, 868, 888
NmuCI	GTSAC	2 cut(s)	375, 418
NsiI	ATGCAT	1 cut(s)	193
NspI	RCATGY	4 cut(s)	296, 827, 837, 888
PaeI	GCATGC	2 cut(s)	296, 827
PagI	TCATGA	1 cut(s)	459
PciI	ACATGT	1 cut(s)	884
PctI	GAATGC	2 cut(s)	45, 191
PfeI	GAWTC	1 cut(s)	352
PflMI	CCANNNNNTGG	1 cut(s)	28
PkrI	GCNGC	3 cut(s)	90, 318, 445
PscI	ACATGT	1 cut(s)	884
PshAI	GACNNNNGTC	1 cut(s)	392
Psp6I	CCWGG	1 cut(s)	868
PspGI	CCWGG	1 cut(s)	868
PspPI	GGNCC	2 cut(s)	383, 625
PsuI	RGATCY	1 cut(s)	568
RsaI	GTAC	3 cut(s)	641, 741, 778
RsaNI	GTAC	3 cut(s)	640, 740, 777
RseI	CAYNNNNRTG	2 cut(s)	464, 832
SaqAI	TTAA	3 cut(s)	452, 504, 716
SatI	GCNGC	3 cut(s)	89, 317, 444
Sau3AI	GATC	3 cut(s)	210, 568, 849
Sau96I	GGNCC	2 cut(s)	383, 625
ScaI	AGTACT	1 cut(s)	778
ScrFI	CCNGG	1 cut(s)	870
SetI	ASST	9 cut(s)	85, 276, 451, 579, 675, 735, 741, 757, 764
SfaNI	GCATC	2 cut(s)	17, 772
SmiMI	CAYNNNNRTG	2 cut(s)	464, 832
SmlI	CTYRAG	2 cut(s)	197, 563
SmoI	CTYRAG	2 cut(s)	197, 563
SphI	GCATGC	2 cut(s)	296, 827
Sse9I	AATT	4 cut(s)	267, 505, 657, 711
SsiI	CCGC	3 cut(s)	86, 391, 475
SspMI	CTAG	2 cut(s)	50, 80
StyD4I	CCNGG	1 cut(s)	868
TaiI	ACGT	1 cut(s)	579
TaqI	TCGA	3 cut(s)	122, 396, 855
TasI	AATT	4 cut(s)	267, 505, 657, 711
TatI	WGTACW	2 cut(s)	639, 776
TfiI	GAWTC	1 cut(s)	352
Tru1I	TTAA	3 cut(s)	452, 504, 716
Tru9I	TTAA	3 cut(s)	452, 504, 716
TscAI	CASTG	3 cut(s)	172, 439, 814
TseFI	GTSAC	2 cut(s)	375, 418
TseI	GCWGC	3 cut(s)	88, 316, 443
Tsp45I	GTSAC	2 cut(s)	375, 418
TspDTI	ATGAA	2 cut(s)	58, 484
TspGWI	ACGGA	1 cut(s)	140
TspRI	CASTG	3 cut(s)	172, 439, 814
Van91I	CCANNNNNTGG	1 cut(s)	28
XapI	RAATTY	1 cut(s)	711
XceI	RCATGY	4 cut(s)	296, 827, 837, 888
XcmI	CCANNNNNNNNNTGG	1 cut(s)	658
XmiI	GTMKAC	1 cut(s)	305
XspI	CTAG	2 cut(s)	50, 80
ZrmI	AGTACT	1 cut(s)	778
Zsp2I	ATGCAT	1 cut(s)	193

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.