Rh7AG102200

Mediates both low-affinity uptake and efflux of sugar across the membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0014431
Physical Location & Seq
Forward (+)
7949312 .. 7951600
2289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG102200.1

Sequence Viewer

Length: 855 bp
ATGACGTCAAGTACTGCTCACTCCCCTCTGGTTTTTGCCTTTGGCATCCTAGGTAACATTGTCTCCTTCATCGTGTTTCTAGCTCCAGTGCCAACATTTTTCAGGGTGTACAAGAAGAAATCAACAGAAGGATTTCAATCAGTTCCATATGTATTTGCACTCTTCAGTGCAACGATCTGGATTTACTATGCATCCCTTAAATCTGATGAGATGCTTCTTATCACCATCAATGGCTTCGGTTGTGTCATCGAGACCATTTATATTGCAATGTACATTACATTTGCACCAAAGCAAGCTAGGGTGAACACCTTGAGGCTGCTTCTTCTTGTGAATTTTGGGGGATTTTGCTTGATTCTTCTTTTGTCTCACTTCCTAGCACAAGGGCCAACTCGGGTTAAAGTTCTTGGATGGGTTTGTGTAGCTTTCTCTGTCAGTGTCTTTGCGGCACCTTTAAGCATCATGATGATACTGTATGCAATTTACCGAAACAGCAAAACGGTTATTGTGGACAAGGAGAAGCTACCAGAGCACAAAACAGCTGATGACATTGTGAAGCAGATCAACATCACGGTTTTGCCTACTTCTGAGGTACAAGTACAAGTAAAAGAAGAGGCCGTAAGCCCCGCCCGTGCCAATAATTCCGACGAAAATGATGATCATGAGCAAAACGAACACGATCAACGTGAGCATGTTCCACAGCCATGTCGCGTTGATGCTCAAGGCATCGACTTGGTCTCTAAGCAAGTCCCGGTATTCATGTGTATGTGTCTATCTAGATATTATAATGGTTATAATGGTGGTGCGTCTGCTTTGGGAGTATTTCAAAAGGTTTCCATTGACGACCGGAATATTTGA

Protein Analysis

284

Amino Acids

31.69

Weight (kDa)

6.99

Isoelectric Point (pI)

37.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MtN3_slv PF03083 13 - 98 9.7e-27 Sugar efflux transporter for intercellular exchange
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014431)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48740 AT5G23660
fragaria_vesca FvH4_5g08970
malus_domestica MD06G1136500.v1.1 MD14G1151300.v1.1
prunus_persica Prupe.5G146400_v2.0.a1
pyrus_communis pycom14g12600 pycom14g12610
rosa_chinensis RchiOBHm_Chr7g0189331
rosa_laevigata RLG00000004618
rosa_multiflora Rmu_sc0001282.1_g000001
rosa_roxburghii Rroxscaffold_3G00265530
rosa_rugosa Rorug06G0497200
rosa_samantha Rh7AG102200 Rh7BG104800 Rh7DG103800
rosa_wichuraiana Rw7G008830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 783, 792
AatII GACGTC 1 cut(s) 8
AccB1I GGYRCC 1 cut(s) 445
AccII CGCG 1 cut(s) 708
AciI CCGC 2 cut(s) 443, 624
AcsI RAATTY 1 cut(s) 331
AcuI CTGAAG 1 cut(s) 148
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 5 cut(s) 13, 110, 272, 591, 597
AgsI TTSAA 2 cut(s) 137, 824
AluBI AGCT 5 cut(s) 83, 296, 422, 520, 539
AluI AGCT 5 cut(s) 83, 296, 422, 520, 539
Alw21I GWGCWC 1 cut(s) 531
Alw26I GTCTC 4 cut(s) 67, 245, 369, 739
Ama87I CYCGRG 1 cut(s) 390
AoxI GGCC 2 cut(s) 383, 612
ApeKI GCWGC 1 cut(s) 316
ApoI RAATTY 1 cut(s) 331
Asp700I GAANNNNTTC 1 cut(s) 132
AspA2I CCTAGG 1 cut(s) 49
AspS9I GGNCC 1 cut(s) 383
AsuC2I CCSGG 1 cut(s) 749
AsuHPI GGTGA 2 cut(s) 214, 313
AvaI CYCGRG 1 cut(s) 390
AvrII CCTAGG 1 cut(s) 49
BanI GGYRCC 1 cut(s) 445
Bbv12I GWGCWC 1 cut(s) 531
BbvI GCAGC 1 cut(s) 303
BccI CCATC 2 cut(s) 233, 402
BceAI ACGGC 1 cut(s) 599
BclI TGATCA 1 cut(s) 655
BcnI CCSGG 1 cut(s) 749
BcoDI GTCTC 4 cut(s) 67, 245, 369, 739
BfaI CTAG 5 cut(s) 50, 80, 297, 374, 774
BisI GCNGC 2 cut(s) 317, 444
BlnI CCTAGG 1 cut(s) 49
BlsI GCNGC 2 cut(s) 318, 445
BmcAI AGTACT 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 749
BmeT110I CYCGRG 1 cut(s) 390
BmgT120I GGNCC 1 cut(s) 383
BmiI GGNNCC 1 cut(s) 447
BmrFI CCNGG 1 cut(s) 749
BmsI GCATC 6 cut(s) 54, 200, 201, 465, 703, 732
BpmI CTGGAG 1 cut(s) 69
BpuEI CTTGAG 2 cut(s) 331, 702
BpuMI CCSGG 1 cut(s) 749
BsaBI GATNNNNATC 2 cut(s) 136, 563
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 245, 739
BsaJI CCNNGG 1 cut(s) 49
BsaWI WCCGGW 1 cut(s) 843
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bse1I ACTGG 1 cut(s) 86
Bse3DI GCAATG 1 cut(s) 273
Bse8I GATNNNNATC 2 cut(s) 136, 563
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 3 cut(s) 45, 191, 413
BseJI GATNNNNATC 2 cut(s) 136, 563
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 1 cut(s) 576
BseNI ACTGG 1 cut(s) 86
BseXI GCAGC 1 cut(s) 303
Bsh1236I CGCG 1 cut(s) 708
Bsh1285I CGRYCG 1 cut(s) 844
BshFI GGCC 2 cut(s) 385, 614
BshNI GGYRCC 1 cut(s) 445
BsiEI CGRYCG 1 cut(s) 844
BsiHKAI GWGCWC 1 cut(s) 531
BsiHKCI CYCGRG 1 cut(s) 390
BsiSI CCGG 2 cut(s) 749, 844
BslFI GGGAC 1 cut(s) 731
BsmAI GTCTC 4 cut(s) 67, 245, 369, 739
BsmFI GGGAC 1 cut(s) 731
BsnI GGCC 2 cut(s) 385, 614
Bso31I GGTCTC 2 cut(s) 245, 739
BsoBI CYCGRG 1 cut(s) 390
Bsp1286I GDGCHC 1 cut(s) 531
Bsp1407I TGTACA 2 cut(s) 108, 270
Bsp143I GATC 4 cut(s) 174, 558, 655, 676
BspACI CCGC 2 cut(s) 443, 624
BspANI GGCC 2 cut(s) 385, 614
BspCNI CTCAG 1 cut(s) 577
BspFNI CGCG 1 cut(s) 708
BspHI TCATGA 2 cut(s) 459, 658
BspLI GGNNCC 1 cut(s) 447
BspT107I GGYRCC 1 cut(s) 445
BspTNI GGTCTC 2 cut(s) 245, 739
BsrDI GCAATG 1 cut(s) 273
BsrGI TGTACA 2 cut(s) 108, 270
BsrI ACTGG 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 4 cut(s) 174, 558, 655, 676
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 49
Bst4CI ACNGT 3 cut(s) 471, 499, 571
Bst6I CTCTTC 2 cut(s) 167, 603
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 2 cut(s) 108, 270
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 2 cut(s) 585, 738
BstF5I GGATG 3 cut(s) 45, 191, 413
BstFNI CGCG 1 cut(s) 708
BstKTI GATC 4 cut(s) 177, 561, 658, 679
BstMAI GTCTC 4 cut(s) 67, 245, 369, 739
BstMBI GATC 4 cut(s) 174, 558, 655, 676
BstMCI CGRYCG 1 cut(s) 844
BstMWI GCNNNNNNNGC 1 cut(s) 526
BstNSI RCATGY 1 cut(s) 692
BstSCI CCNGG 1 cut(s) 747
BstUI CGCG 1 cut(s) 708
BstV1I GCAGC 1 cut(s) 303
BsuRI GGCC 2 cut(s) 385, 614
BtsCI GGATG 3 cut(s) 45, 191, 413
BtsIMutI CAGTG 3 cut(s) 93, 172, 439
Cac8I GCNNGC 1 cut(s) 294
CciI TCATGA 2 cut(s) 459, 658
Cfr13I GGNCC 1 cut(s) 383
CseI GACGC 1 cut(s) 792
Csp6I GTAC 5 cut(s) 12, 109, 271, 590, 596
CviAII CATG 5 cut(s) 460, 659, 689, 702, 757
CviQI GTAC 5 cut(s) 12, 109, 271, 590, 596
DdeI CTNAG 2 cut(s) 585, 738
DpnI GATC 4 cut(s) 176, 560, 657, 678
DpnII GATC 4 cut(s) 174, 558, 655, 676
Eam1104I CTCTTC 2 cut(s) 167, 603
EarI CTCTTC 2 cut(s) 167, 603
Eco130I CCWWGG 1 cut(s) 49
Eco31I GGTCTC 2 cut(s) 245, 739
Eco57I CTGAAG 1 cut(s) 148
Eco88I CYCGRG 1 cut(s) 390
EcoT14I CCWWGG 1 cut(s) 49
EcoT22I ATGCAT 1 cut(s) 193
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 5 cut(s) 463, 662, 692, 705, 760
FaqI GGGAC 1 cut(s) 731
FatI CATG 5 cut(s) 459, 658, 688, 701, 756
FauI CCCGC 1 cut(s) 631
FauNDI CATATG 1 cut(s) 148
FbaI TGATCA 1 cut(s) 655
Fnu4HI GCNGC 2 cut(s) 317, 444
FokI GGATG 3 cut(s) 32, 178, 420
Fsp4HI GCNGC 2 cut(s) 317, 444
FspBI CTAG 5 cut(s) 50, 80, 297, 374, 774
GluI GCNGC 2 cut(s) 317, 444
GsuI CTGGAG 1 cut(s) 69
HaeIII GGCC 2 cut(s) 385, 614
HapII CCGG 2 cut(s) 749, 844
HgaI GACGC 1 cut(s) 792
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 463, 662, 692, 705, 760
HinfI GANTC 1 cut(s) 352
HpaII CCGG 2 cut(s) 749, 844
HphI GGTGA 2 cut(s) 214, 313
Hpy166II GTNNAC 3 cut(s) 109, 304, 508
Hpy188I TCNGA 3 cut(s) 205, 586, 643
Hpy188III TCNNGA 5 cut(s) 178, 250, 460, 659, 774
Hpy8I GTNNAC 3 cut(s) 109, 304, 508
Hpy99I CGWCG 1 cut(s) 647
HpyAV CCTTC 2 cut(s) 76, 122
HpyCH4III ACNGT 3 cut(s) 471, 499, 571
HpyCH4IV ACGT 2 cut(s) 5, 682
HpyCH4V TGCA 6 cut(s) 158, 170, 191, 266, 284, 476
HpyF10VI GCNNNNNNNGC 1 cut(s) 526
HpyF3I CTNAG 2 cut(s) 585, 738
HpySE526I ACGT 2 cut(s) 5, 682
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 463, 662, 692, 705, 760
Ksp22I TGATCA 1 cut(s) 655
Kzo9I GATC 4 cut(s) 174, 558, 655, 676
LmnI GCTCC 1 cut(s) 88
LpnPI CCDG 6 cut(s) 14, 88, 99, 163, 537, 762
Lsp1109I GCAGC 1 cut(s) 303
LweI GCATC 6 cut(s) 54, 200, 201, 465, 703, 732
MaeI CTAG 5 cut(s) 50, 80, 297, 374, 774
MaeII ACGT 2 cut(s) 5, 682
MaeIII GTNAC 1 cut(s) 53
MalI GATC 4 cut(s) 176, 560, 657, 678
MboI GATC 4 cut(s) 174, 558, 655, 676
MboII GAAGA 5 cut(s) 127, 154, 314, 347, 620
MhlI GDGCHC 1 cut(s) 531
MluCI AATT 3 cut(s) 331, 477, 637
MmeI TCCRAC 1 cut(s) 666
MnlI CCTC 4 cut(s) 36, 306, 580, 604
Mph1103I ATGCAT 1 cut(s) 193
MroXI GAANNNNTTC 1 cut(s) 132
MseI TTAA 3 cut(s) 198, 396, 452
MslI CAYNNNNRTG 3 cut(s) 461, 700, 761
MspA1I CMGCKG 1 cut(s) 539
MspI CCGG 2 cut(s) 749, 844
MspR9I CCNGG 1 cut(s) 749
MvnI CGCG 1 cut(s) 708
MwoI GCNNNNNNNGC 1 cut(s) 526
NciI CCSGG 1 cut(s) 749
NdeI CATATG 1 cut(s) 148
NdeII GATC 4 cut(s) 174, 558, 655, 676
NlaIII CATG 5 cut(s) 463, 662, 692, 705, 760
NlaIV GGNNCC 1 cut(s) 447
NsiI ATGCAT 1 cut(s) 193
NspI RCATGY 1 cut(s) 692
PagI TCATGA 2 cut(s) 459, 658
PdmI GAANNNNTTC 1 cut(s) 132
PfeI GAWTC 1 cut(s) 352
PflFI GACNNNGTC 1 cut(s) 731
PkrI GCNGC 2 cut(s) 318, 445
PsiI TTATAA 2 cut(s) 783, 792
PspN4I GGNNCC 1 cut(s) 447
PspPI GGNCC 1 cut(s) 383
PsyI GACNNNGTC 1 cut(s) 731
PvuII CAGCTG 1 cut(s) 539
RsaI GTAC 5 cut(s) 13, 110, 272, 591, 597
RsaNI GTAC 5 cut(s) 12, 109, 271, 590, 596
RseI CAYNNNNRTG 3 cut(s) 461, 700, 761
SaqAI TTAA 3 cut(s) 198, 396, 452
SatI GCNGC 2 cut(s) 317, 444
Sau3AI GATC 4 cut(s) 174, 558, 655, 676
Sau96I GGNCC 1 cut(s) 383
ScaI AGTACT 1 cut(s) 13
ScrFI CCNGG 1 cut(s) 749
SduI GDGCHC 1 cut(s) 531
SfaNI GCATC 6 cut(s) 54, 200, 201, 465, 703, 732
SmiMI CAYNNNNRTG 3 cut(s) 461, 700, 761
SmlI CTYRAG 2 cut(s) 310, 717
SmoI CTYRAG 2 cut(s) 310, 717
Sse9I AATT 3 cut(s) 331, 477, 637
SsiI CCGC 2 cut(s) 443, 624
SspI AATATT 1 cut(s) 850
SspMI CTAG 5 cut(s) 50, 80, 297, 374, 774
StyD4I CCNGG 1 cut(s) 747
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 3 cut(s) 471, 499, 571
TaiI ACGT 2 cut(s) 8, 685
TaqI TCGA 2 cut(s) 249, 726
TasI AATT 3 cut(s) 331, 477, 637
TatI WGTACW 4 cut(s) 11, 108, 270, 595
TauI GCSGC 1 cut(s) 446
TfiI GAWTC 1 cut(s) 352
Tru1I TTAA 3 cut(s) 198, 396, 452
Tru9I TTAA 3 cut(s) 198, 396, 452
TscAI CASTG 3 cut(s) 93, 172, 439
TseI GCWGC 1 cut(s) 316
TspDTI ATGAA 2 cut(s) 58, 745
TspRI CASTG 3 cut(s) 93, 172, 439
Tth111I GACNNNGTC 1 cut(s) 731
XapI RAATTY 1 cut(s) 331
XbaI TCTAGA 1 cut(s) 773
XceI RCATGY 1 cut(s) 692
XmaJI CCTAGG 1 cut(s) 49
XmnI GAANNNNTTC 1 cut(s) 132
XspI CTAG 5 cut(s) 50, 80, 297, 374, 774
ZraI GACGTC 1 cut(s) 6
ZrmI AGTACT 1 cut(s) 13
Zsp2I ATGCAT 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.