MD14G1156400.v1.1

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006761
Physical Location & Seq
Forward (+)
25090589 .. 25093134
2546 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1156400.v1.1.491

Sequence Viewer

Length: 717 bp
ATGGACCCCAACCCCAACACTGCCACCACCGCTGCCGCCGCTGCCCCCACCCAGCAGCAACCGCAAGCCCAATCCTCCCCCTACCCAGCAACCCAATCCTCCGTCCCCGCCCCCCCATTCCACCACCTCCTCCAGCAGCAGCAGCAGCAGCTCCAGATGTTCTGGACCTTCCAGCGCCAGGAGATTGAGAGCGTCAACGACTTCAAGAACCACCAGCTCCCCCTTGCCCGCATCAAGAAGATCATGAAGGCCGACGAGGACGTCCGTATGATCTCCGCCGAGGCCCCCGTCCTCTTCGCCAAGGCCTGCGAGCTCTTCATCCTCGAGCTCACCATCCGCTCCTGGCTCCACGCGGAGGAGAACAAGCGCCGGACTCTCCAGAAAAACGACATTGCCGCCGCCATTACCCGCACCGACATATTCGATTTCTTGGTGGATATTGTGCCCCGCGACGAGATCAAGGAGGAGGCGGTGGGTCTCGGAGGTATGGTCGGGGCGACGGCGAGTGGTGTGCCATATTTCTATCCGCCGATGGGTCAGCCGGCTGGTGGCCCCGGGGGGATGATGATTGGGAGGCCTGCGGTGGACCCCACTGGGGTTTACGGCGTGCAGCCTCCGTCGCAGGCGTGGCAGTCGGTGTGGCAGACTGCGGCGGATGATGGGTCGTACGGGAGTGGAGGGAGCAGTGGGCAGGGCAACCTTGATGGTCAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0005737 GO:0005829 GO:0006109 GO:0006355 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009739 GO:0009740 GO:0009755 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010029 GO:0010033 GO:0010099 GO:0010468 GO:0010476 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010628 GO:0010675 GO:0010677 GO:0016602 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0032870 GO:0032881 GO:0032991 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044798 GO:0045893 GO:0045912 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048586 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0080090 GO:0090575 GO:0097305 GO:0097306 GO:0140110 GO:1900140 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000028 GO:2000030 GO:2000112 GO:2000241 GO:2000306 GO:2000904 GO:2000905 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04612 ko05152 map04612 map05152
Pfam Domains
Protein Families
CBFD_NFYB_HMF

Protein Analysis

239

Amino Acids

25.78

Weight (kDa)

5.05

Isoelectric Point (pI)

64.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 64 - 135 1.3e-17 Core histone H2A/H2B/H3/H4 domain
CBFD_NFYB_HMF PF00808 72 - 135 9.9e-17 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 260
AatII GACGTC 1 cut(s) 264
AccBSI CCGCTC 1 cut(s) 339
AccII CGCG 2 cut(s) 353, 450
AcyI GRCGYC 1 cut(s) 261
AfaI GTAC 1 cut(s) 668
AfiI CCNNNNNNNGG 5 cut(s) 178, 355, 533, 548, 595
AgsI TTSAA 1 cut(s) 205
AjnI CCWGG 2 cut(s) 177, 341
AluBI AGCT 4 cut(s) 151, 217, 313, 328
AluI AGCT 4 cut(s) 151, 217, 313, 328
Alw21I GWGCWC 2 cut(s) 315, 330
Alw26I GTCTC 1 cut(s) 482
Ama87I CYCGRG 2 cut(s) 323, 554
AoxI GGCC 5 cut(s) 249, 282, 303, 550, 575
ApeKI GCWGC 9 cut(s) 32, 41, 55, 136, 139, 142, 145, 148, 610
AspLEI GCGC 2 cut(s) 177, 369
AspS9I GGNCC 5 cut(s) 4, 165, 283, 551, 586
AsuC2I CCSGG 2 cut(s) 555, 556
AsuHPI GGTGA 1 cut(s) 322
AvaI CYCGRG 2 cut(s) 323, 554
AvaII GGWCC 3 cut(s) 4, 165, 586
BaeGI GKGCMC 1 cut(s) 447
BanII GRGCYC 2 cut(s) 315, 330
Bbv12I GWGCWC 2 cut(s) 315, 330
BbvI GCAGC 9 cut(s) 19, 28, 67, 148, 151, 154, 157, 160, 622
BccI CCATC 4 cut(s) 341, 526, 653, 698
BceAI ACGGC 2 cut(s) 516, 619
BcgI CGANNNNNNTGC 2 cut(s) 493, 527
BciT130I CCWGG 2 cut(s) 179, 343
BcnI CCSGG 2 cut(s) 555, 556
BcoDI GTCTC 1 cut(s) 482
BfoI RGCGCY 2 cut(s) 178, 370
Bme1390I CCNGG 4 cut(s) 179, 343, 555, 556
Bme18I GGWCC 3 cut(s) 4, 165, 586
BmeT110I CYCGRG 2 cut(s) 323, 554
BmgT120I GGNCC 5 cut(s) 4, 165, 283, 551, 586
BmiI GGNNCC 5 cut(s) 6, 285, 347, 553, 588
BmrFI CCNGG 4 cut(s) 179, 343, 555, 556
BmrI ACTGGG 1 cut(s) 603
BmsI GCATC 1 cut(s) 240
BmuI ACTGGG 1 cut(s) 603
BpmI CTGGAG 3 cut(s) 116, 137, 362
BpuMI CCSGG 2 cut(s) 555, 556
BsaHI GRCGYC 1 cut(s) 261
BsaI GGTCTC 1 cut(s) 482
BsaJI CCNNGG 5 cut(s) 279, 300, 553, 554, 555
BsaXI ACNNNNNCTCC 2 cut(s) 474, 504
Bsc4I CCNNNNNNNGG 5 cut(s) 178, 355, 533, 548, 595
Bse118I RCCGGY 1 cut(s) 541
Bse1I ACTGG 1 cut(s) 598
Bse3DI GCAATG 1 cut(s) 390
BseBI CCWGG 2 cut(s) 179, 343
BseDI CCNNGG 5 cut(s) 279, 300, 553, 554, 555
BseGI GGATG 4 cut(s) 318, 333, 567, 661
BseLI CCNNNNNNNGG 5 cut(s) 178, 355, 533, 548, 595
BseMI GCAATG 1 cut(s) 390
BseNI ACTGG 1 cut(s) 598
BseRI GAGGAG 3 cut(s) 119, 371, 479
BseSI GKGCMC 1 cut(s) 447
BseXI GCAGC 9 cut(s) 19, 28, 67, 148, 151, 154, 157, 160, 622
BseYI CCCAGC 2 cut(s) 51, 85
BsgI GTGCAG 1 cut(s) 629
Bsh1236I CGCG 2 cut(s) 353, 450
BshFI GGCC 5 cut(s) 251, 284, 305, 552, 577
BsiHKAI GWGCWC 2 cut(s) 315, 330
BsiHKCI CYCGRG 2 cut(s) 323, 554
BsiSI CCGG 3 cut(s) 370, 542, 555
BsiWI CGTACG 1 cut(s) 666
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 5 cut(s) 178, 355, 533, 548, 595
BsmAI GTCTC 1 cut(s) 482
BsmFI GGGAC 1 cut(s) 89
BsnI GGCC 5 cut(s) 251, 284, 305, 552, 577
Bso31I GGTCTC 1 cut(s) 482
BsoBI CYCGRG 2 cut(s) 323, 554
Bsp1286I GDGCHC 3 cut(s) 315, 330, 447
Bsp143I GATC 3 cut(s) 240, 270, 456
BspANI GGCC 5 cut(s) 251, 284, 305, 552, 577
BspFNI CGCG 2 cut(s) 353, 450
BspHI TCATGA 1 cut(s) 243
BspLI GGNNCC 5 cut(s) 6, 285, 347, 553, 588
BspQI GCTCTTC 1 cut(s) 320
BspTNI GGTCTC 1 cut(s) 482
BsrBI CCGCTC 1 cut(s) 339
BsrDI GCAATG 1 cut(s) 390
BsrFI RCCGGY 1 cut(s) 541
BsrI ACTGG 1 cut(s) 598
BssAI RCCGGY 1 cut(s) 541
BssECI CCNNGG 5 cut(s) 279, 300, 553, 554, 555
BssMI GATC 3 cut(s) 240, 270, 456
BssNI GRCGYC 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 300
Bst2UI CCWGG 2 cut(s) 179, 343
Bst6I CTCTTC 2 cut(s) 299, 320
BstACI GRCGYC 1 cut(s) 261
BstC8I GCNNGC 8 cut(s) 66, 229, 307, 311, 543, 579, 608, 624
BstF5I GGATG 4 cut(s) 318, 333, 567, 661
BstFNI CGCG 2 cut(s) 353, 450
BstH2I RGCGCY 2 cut(s) 178, 370
BstHHI GCGC 2 cut(s) 177, 369
BstKTI GATC 3 cut(s) 243, 273, 459
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 3 cut(s) 240, 270, 456
BstMWI GCNNNNNNNGC 9 cut(s) 29, 38, 41, 61, 142, 145, 148, 619, 628
BstNI CCWGG 2 cut(s) 179, 343
BstSCI CCNGG 4 cut(s) 177, 341, 553, 554
BstSLI GKGCMC 1 cut(s) 447
BstUI CGCG 2 cut(s) 353, 450
BstV1I GCAGC 9 cut(s) 19, 28, 67, 148, 151, 154, 157, 160, 622
BsuRI GGCC 5 cut(s) 251, 284, 305, 552, 577
BtsCI GGATG 4 cut(s) 318, 333, 567, 661
BtsI GCAGTG 2 cut(s) 18, 691
BtsIMutI CAGTG 3 cut(s) 18, 591, 691
Cac8I GCNNGC 8 cut(s) 66, 229, 307, 311, 543, 579, 608, 624
CciI TCATGA 1 cut(s) 243
CfoI GCGC 2 cut(s) 177, 369
Cfr10I RCCGGY 1 cut(s) 541
Cfr13I GGNCC 5 cut(s) 4, 165, 283, 551, 586
Cfr9I CCCGGG 1 cut(s) 554
CseI GACGC 1 cut(s) 181
Csp6I GTAC 1 cut(s) 667
CviAII CATG 1 cut(s) 244
CviQI GTAC 1 cut(s) 667
DpnI GATC 3 cut(s) 242, 272, 458
DpnII GATC 3 cut(s) 240, 270, 456
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
Eam1104I CTCTTC 2 cut(s) 299, 320
EarI CTCTTC 2 cut(s) 299, 320
EciI GGCGGA 3 cut(s) 265, 516, 668
Ecl136II GAGCTC 2 cut(s) 313, 328
Eco130I CCWWGG 1 cut(s) 300
Eco147I AGGCCT 2 cut(s) 305, 577
Eco24I GRGCYC 2 cut(s) 315, 330
Eco31I GGTCTC 1 cut(s) 482
Eco47I GGWCC 3 cut(s) 4, 165, 586
Eco53kI GAGCTC 2 cut(s) 313, 328
Eco88I CYCGRG 2 cut(s) 323, 554
EcoICRI GAGCTC 2 cut(s) 313, 328
EcoO109I RGGNCCY 1 cut(s) 283
EcoRII CCWGG 2 cut(s) 177, 341
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 2 cut(s) 315, 330
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 1 cut(s) 247
FaiI YATR 5 cut(s) 245, 269, 419, 488, 517
FaqI GGGAC 1 cut(s) 89
FatI CATG 1 cut(s) 243
FauI CCCGC 4 cut(s) 115, 236, 416, 455
FokI GGATG 4 cut(s) 305, 320, 574, 668
FriOI GRGCYC 2 cut(s) 315, 330
GlaI GCGC 2 cut(s) 176, 368
GsaI CCCAGC 2 cut(s) 55, 89
GsuI CTGGAG 3 cut(s) 116, 137, 362
HaeII RGCGCY 2 cut(s) 178, 370
HaeIII GGCC 5 cut(s) 251, 284, 305, 552, 577
HapII CCGG 3 cut(s) 370, 542, 555
HgaI GACGC 1 cut(s) 181
HhaI GCGC 2 cut(s) 177, 369
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 1 cut(s) 247
Hin6I GCGC 2 cut(s) 175, 367
HinP1I GCGC 2 cut(s) 175, 367
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 1 cut(s) 373
HpaII CCGG 3 cut(s) 370, 542, 555
HphI GGTGA 1 cut(s) 322
Hpy166II GTNNAC 3 cut(s) 196, 586, 601
Hpy188I TCNGA 1 cut(s) 482
Hpy188III TCNNGA 6 cut(s) 154, 163, 205, 235, 244, 379
Hpy8I GTNNAC 3 cut(s) 196, 586, 601
Hpy99I CGWCG 4 cut(s) 257, 455, 502, 622
HpyAV CCTTC 2 cut(s) 178, 241
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 1 cut(s) 610
HpyF10VI GCNNNNNNNGC 9 cut(s) 29, 38, 41, 61, 142, 145, 148, 619, 628
HpySE526I ACGT 1 cut(s) 261
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 1 cut(s) 247
HspAI GCGC 2 cut(s) 175, 367
KroI GCCGGC 1 cut(s) 541
KroNI GCCGGC 1 cut(s) 543
Kzo9I GATC 3 cut(s) 240, 270, 456
LguI GCTCTTC 1 cut(s) 320
LmnI GCTCC 5 cut(s) 156, 222, 344, 351, 681
Lsp1109I GCAGC 9 cut(s) 19, 28, 67, 148, 151, 154, 157, 160, 622
LweI GCATC 1 cut(s) 240
MaeII ACGT 1 cut(s) 261
MalI GATC 3 cut(s) 242, 272, 458
MbiI CCGCTC 1 cut(s) 339
MboI GATC 3 cut(s) 240, 270, 456
MboII GAAGA 3 cut(s) 250, 286, 307
MhlI GDGCHC 3 cut(s) 315, 330, 447
MlyI GAGTC 1 cut(s) 367
MroNI GCCGGC 1 cut(s) 541
MspA1I CMGCKG 2 cut(s) 32, 41
MspI CCGG 3 cut(s) 370, 542, 555
MspR9I CCNGG 4 cut(s) 179, 343, 555, 556
MvaI CCWGG 2 cut(s) 179, 343
MvnI CGCG 2 cut(s) 353, 450
MwoI GCNNNNNNNGC 9 cut(s) 29, 38, 41, 61, 142, 145, 148, 619, 628
NaeI GCCGGC 1 cut(s) 543
NciI CCSGG 2 cut(s) 555, 556
NdeII GATC 3 cut(s) 240, 270, 456
NgoMIV GCCGGC 1 cut(s) 541
NlaIII CATG 1 cut(s) 247
NlaIV GGNNCC 5 cut(s) 6, 285, 347, 553, 588
NmeAIII GCCGAG 1 cut(s) 304
PaeR7I CTCGAG 1 cut(s) 323
PagI TCATGA 1 cut(s) 243
PceI AGGCCT 2 cut(s) 305, 577
PciSI GCTCTTC 1 cut(s) 320
PdiI GCCGGC 1 cut(s) 543
Pfl23II CGTACG 1 cut(s) 666
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
Psp124BI GAGCTC 2 cut(s) 315, 330
Psp6I CCWGG 2 cut(s) 177, 341
PspFI CCCAGC 2 cut(s) 51, 85
PspGI CCWGG 2 cut(s) 177, 341
PspLI CGTACG 1 cut(s) 666
PspN4I GGNNCC 5 cut(s) 6, 285, 347, 553, 588
PspPI GGNCC 5 cut(s) 4, 165, 283, 551, 586
PspXI VCTCGAGB 1 cut(s) 323
RsaI GTAC 1 cut(s) 668
RsaNI GTAC 1 cut(s) 667
SacI GAGCTC 2 cut(s) 315, 330
SapI GCTCTTC 1 cut(s) 320
Sau3AI GATC 3 cut(s) 240, 270, 456
Sau96I GGNCC 5 cut(s) 4, 165, 283, 551, 586
SchI GAGTC 1 cut(s) 367
ScrFI CCNGG 4 cut(s) 179, 343, 555, 556
SduI GDGCHC 3 cut(s) 315, 330, 447
SetI ASST 9 cut(s) 129, 153, 170, 219, 264, 315, 330, 487, 702
SfaNI GCATC 1 cut(s) 240
Sfr274I CTCGAG 1 cut(s) 323
SinI GGWCC 3 cut(s) 4, 165, 586
SlaI CTCGAG 1 cut(s) 323
SmaI CCCGGG 1 cut(s) 556
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
SseBI AGGCCT 2 cut(s) 305, 577
SstI GAGCTC 2 cut(s) 315, 330
StuI AGGCCT 2 cut(s) 305, 577
StyD4I CCNGG 4 cut(s) 177, 341, 553, 554
StyI CCWWGG 1 cut(s) 300
TaiI ACGT 1 cut(s) 264
TaqI TCGA 2 cut(s) 324, 423
TauI GCSGC 5 cut(s) 38, 41, 398, 401, 653
TscAI CASTG 3 cut(s) 25, 598, 691
TseI GCWGC 9 cut(s) 32, 41, 55, 136, 139, 142, 145, 148, 610
TspDTI ATGAA 2 cut(s) 260, 307
TspGWI ACGGA 3 cut(s) 91, 254, 606
TspMI CCCGGG 1 cut(s) 554
TspRI CASTG 3 cut(s) 25, 598, 691
VpaK11BI GGWCC 3 cut(s) 4, 165, 586
XhoI CTCGAG 1 cut(s) 323
XmaI CCCGGG 1 cut(s) 554
ZraI GACGTC 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.