pycom06g13120

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0006761
Physical Location & Seq
Forward (+)
18182876 .. 18185412
2537 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g13120.1

Sequence Viewer

Length: 720 bp
ATGGACCCCAACCCCAACTCCGGCGCACCCACCTCCGCCGCCGCCGCCCCGACCCAGCAACCACCGCAAGCCCAATCCTCCCCTTACGGAGCAACCCAATCCTCCGTCCCCGCCCTCCCATTCCACCACCTCCTCCAGCAGCAGCAGCAGCAGCTCCAGATGTTCTGGACCTACCAGCGCCACGAGATTGAGAGCGTCAACGACTTCAAGAACCACCAGCTGCCGCTTGCCCGCATCAAGAAGATCATGAAGGCCGACGAGGACGTCCGCATGATCTCCGCCGAGGCCCCCGTCCTCTTCGCCAAAGCCTGCGAGCTCTTCATCCTCGAGCTCACCATCCGCTCCTGGCTCCACGCCGAGGAGAACAAGCGCCGGACCCTCCAGAAAAACGACATTGCCGCTGCCATTACCCGCACCGACATATTCGATTTCTTGGTGGATATAGTGCCCCGCGACGAGATCAAGGAGGAGGCGGTGGGGCTCGGAGGGATGGTCGGGGCGACGGCTAGTGGGGTGCCGTACTACTATCCGCCGATGGGGCAGCCGGCTGGTGGCCCCGGAGGGATGATGATAGGGAGGCCTGCGGTGGACCCTGCAGGGGTTTACGGCGTGCAGCCGCCGTCGCAGGCGTGGCAGTCGGTGTGGCAGACGGCGGCGGATGATGGGTCGTACGGGAGTGGAGGGAGCAGTGGACAGGGCAACCTCGACGGTCAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0005737 GO:0005829 GO:0006109 GO:0006355 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009739 GO:0009740 GO:0009755 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010029 GO:0010033 GO:0010099 GO:0010468 GO:0010476 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010628 GO:0010675 GO:0010677 GO:0016602 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0032870 GO:0032881 GO:0032991 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044798 GO:0045893 GO:0045912 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048586 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0080090 GO:0090575 GO:0097305 GO:0097306 GO:0140110 GO:1900140 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000028 GO:2000030 GO:2000112 GO:2000241 GO:2000306 GO:2000904 GO:2000905 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04612 ko05152 map04612 map05152
Pfam Domains
Protein Families
CBFD_NFYB_HMF

Protein Analysis

240

Amino Acids

25.79

Weight (kDa)

5.17

Isoelectric Point (pI)

62.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 65 - 136 7.8e-18 Core histone H2A/H2B/H3/H4 domain
CBFD_NFYB_HMF PF00808 73 - 136 1e-16 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 263
AatII GACGTC 1 cut(s) 267
AccB1I GGYRCC 1 cut(s) 514
AccBSI CCGCTC 1 cut(s) 342
AccII CGCG 1 cut(s) 453
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 2 cut(s) 521, 671
AfiI CCNNNNNNNGG 5 cut(s) 20, 358, 536, 551, 598
AgsI TTSAA 1 cut(s) 208
AjnI CCWGG 1 cut(s) 344
AluBI AGCT 4 cut(s) 154, 220, 316, 331
AluI AGCT 4 cut(s) 154, 220, 316, 331
Alw21I GWGCWC 2 cut(s) 318, 333
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 4 cut(s) 252, 285, 553, 578
ApeKI GCWGC 9 cut(s) 139, 142, 145, 148, 151, 220, 401, 541, 613
AspLEI GCGC 3 cut(s) 26, 180, 372
AspS9I GGNCC 6 cut(s) 4, 168, 286, 375, 554, 589
AsuC2I CCSGG 1 cut(s) 558
AsuHPI GGTGA 1 cut(s) 325
AvaI CYCGRG 1 cut(s) 326
AvaII GGWCC 4 cut(s) 4, 168, 375, 589
BaeGI GKGCMC 1 cut(s) 450
BanI GGYRCC 1 cut(s) 514
BanII GRGCYC 3 cut(s) 318, 333, 483
BauI CACGAG 1 cut(s) 182
Bbv12I GWGCWC 2 cut(s) 318, 333
BbvI GCAGC 9 cut(s) 151, 154, 157, 160, 163, 207, 388, 553, 625
BccI CCATC 4 cut(s) 344, 484, 529, 656
BceAI ACGGC 5 cut(s) 502, 519, 604, 622, 666
BciT130I CCWGG 1 cut(s) 346
BcnI CCSGG 1 cut(s) 558
BfaI CTAG 1 cut(s) 507
BfmI CTRYAG 1 cut(s) 594
BfoI RGCGCY 2 cut(s) 181, 373
BglI GCCNNNNNGGC 1 cut(s) 538
Bme1390I CCNGG 2 cut(s) 346, 558
Bme18I GGWCC 4 cut(s) 4, 168, 375, 589
BmeT110I CYCGRG 1 cut(s) 326
BmgT120I GGNCC 6 cut(s) 4, 168, 286, 375, 554, 589
BmiI GGNNCC 7 cut(s) 6, 288, 350, 377, 516, 556, 591
BmrFI CCNGG 2 cut(s) 346, 558
BmsI GCATC 1 cut(s) 243
BpmI CTGGAG 3 cut(s) 119, 140, 365
BpuMI CCSGG 1 cut(s) 558
BsaHI GRCGYC 1 cut(s) 264
BsaJI CCNNGG 3 cut(s) 282, 357, 556
BsaXI ACNNNNNCTCC 3 cut(s) 32, 477, 507
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 358, 536, 551, 598
Bse118I RCCGGY 1 cut(s) 544
Bse3DI GCAATG 1 cut(s) 393
BseBI CCWGG 1 cut(s) 346
BseDI CCNNGG 3 cut(s) 282, 357, 556
BseGI GGATG 5 cut(s) 321, 336, 495, 570, 664
BseLI CCNNNNNNNGG 5 cut(s) 20, 358, 536, 551, 598
BseMI GCAATG 1 cut(s) 393
BseRI GAGGAG 3 cut(s) 122, 374, 482
BseSI GKGCMC 1 cut(s) 450
BseXI GCAGC 9 cut(s) 151, 154, 157, 160, 163, 207, 388, 553, 625
BseYI CCCAGC 1 cut(s) 54
BsgI GTGCAG 1 cut(s) 632
Bsh1236I CGCG 1 cut(s) 453
BshFI GGCC 4 cut(s) 254, 287, 555, 580
BshNI GGYRCC 1 cut(s) 514
BsiHKAI GWGCWC 2 cut(s) 318, 333
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 4 cut(s) 21, 373, 545, 558
BsiWI CGTACG 1 cut(s) 669
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 5 cut(s) 20, 358, 536, 551, 598
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 4 cut(s) 254, 287, 555, 580
BsoBI CYCGRG 1 cut(s) 326
Bsp1286I GDGCHC 4 cut(s) 318, 333, 450, 483
Bsp143I GATC 3 cut(s) 243, 273, 459
BspANI GGCC 4 cut(s) 254, 287, 555, 580
BspFNI CGCG 1 cut(s) 453
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 7 cut(s) 6, 288, 350, 377, 516, 556, 591
BspMAI CTGCAG 1 cut(s) 598
BspQI GCTCTTC 1 cut(s) 323
BspT107I GGYRCC 1 cut(s) 514
BsrBI CCGCTC 1 cut(s) 342
BsrDI GCAATG 1 cut(s) 393
BsrFI RCCGGY 1 cut(s) 544
BssAI RCCGGY 1 cut(s) 544
BssECI CCNNGG 3 cut(s) 282, 357, 556
BssMI GATC 3 cut(s) 243, 273, 459
BssNI GRCGYC 1 cut(s) 264
BssSI CACGAG 1 cut(s) 182
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 346
Bst4CI ACNGT 1 cut(s) 710
Bst6I CTCTTC 2 cut(s) 302, 323
BstACI GRCGYC 1 cut(s) 264
BstC8I GCNNGC 9 cut(s) 69, 228, 232, 310, 314, 546, 582, 611, 627
BstF5I GGATG 5 cut(s) 321, 336, 495, 570, 664
BstFNI CGCG 1 cut(s) 453
BstH2I RGCGCY 2 cut(s) 181, 373
BstHHI GCGC 3 cut(s) 26, 180, 372
BstKTI GATC 3 cut(s) 246, 276, 462
BstMBI GATC 3 cut(s) 243, 273, 459
BstMWI GCNNNNNNNGC 8 cut(s) 44, 64, 145, 148, 151, 538, 622, 631
BstNI CCWGG 1 cut(s) 346
BstSCI CCNGG 2 cut(s) 344, 556
BstSFI CTRYAG 1 cut(s) 594
BstSLI GKGCMC 1 cut(s) 450
BstUI CGCG 1 cut(s) 453
BstV1I GCAGC 9 cut(s) 151, 154, 157, 160, 163, 207, 388, 553, 625
BsuRI GGCC 4 cut(s) 254, 287, 555, 580
BtsCI GGATG 5 cut(s) 321, 336, 495, 570, 664
BtsI GCAGTG 1 cut(s) 694
BtsIMutI CAGTG 1 cut(s) 694
Cac8I GCNNGC 9 cut(s) 69, 228, 232, 310, 314, 546, 582, 611, 627
CciI TCATGA 1 cut(s) 246
CfoI GCGC 3 cut(s) 26, 180, 372
Cfr10I RCCGGY 1 cut(s) 544
Cfr13I GGNCC 6 cut(s) 4, 168, 286, 375, 554, 589
CseI GACGC 1 cut(s) 184
Csp6I GTAC 2 cut(s) 520, 670
CviAII CATG 2 cut(s) 247, 271
CviQI GTAC 2 cut(s) 520, 670
DpnI GATC 3 cut(s) 245, 275, 461
DpnII GATC 3 cut(s) 243, 273, 459
DrdI GACNNNNNNGTC 1 cut(s) 263
DseDI GACNNNNNNGTC 1 cut(s) 263
Eam1104I CTCTTC 2 cut(s) 302, 323
EarI CTCTTC 2 cut(s) 302, 323
EciI GGCGGA 4 cut(s) 25, 268, 519, 671
Ecl136II GAGCTC 2 cut(s) 316, 331
Eco147I AGGCCT 1 cut(s) 580
Eco24I GRGCYC 3 cut(s) 318, 333, 483
Eco47I GGWCC 4 cut(s) 4, 168, 375, 589
Eco53kI GAGCTC 2 cut(s) 316, 331
Eco88I CYCGRG 1 cut(s) 326
EcoICRI GAGCTC 2 cut(s) 316, 331
EcoO109I RGGNCCY 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 344
EcoT38I GRGCYC 3 cut(s) 318, 333, 483
FaeI CATG 2 cut(s) 250, 274
FaiI YATR 4 cut(s) 248, 272, 422, 443
FaqI GGGAC 1 cut(s) 92
FatI CATG 2 cut(s) 246, 270
FauI CCCGC 4 cut(s) 118, 239, 419, 458
FokI GGATG 5 cut(s) 308, 323, 502, 577, 671
FriOI GRGCYC 3 cut(s) 318, 333, 483
FspBI CTAG 1 cut(s) 507
GlaI GCGC 3 cut(s) 25, 179, 371
GsaI CCCAGC 1 cut(s) 58
GsuI CTGGAG 3 cut(s) 119, 140, 365
HaeII RGCGCY 2 cut(s) 181, 373
HaeIII GGCC 4 cut(s) 254, 287, 555, 580
HapII CCGG 4 cut(s) 21, 373, 545, 558
HgaI GACGC 1 cut(s) 184
HhaI GCGC 3 cut(s) 26, 180, 372
Hin1I GRCGYC 1 cut(s) 264
Hin1II CATG 2 cut(s) 250, 274
Hin6I GCGC 3 cut(s) 24, 178, 370
HinP1I GCGC 3 cut(s) 24, 178, 370
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HpaII CCGG 4 cut(s) 21, 373, 545, 558
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 4 cut(s) 199, 589, 604, 692
Hpy188I TCNGA 1 cut(s) 485
Hpy188III TCNNGA 6 cut(s) 157, 166, 208, 238, 247, 382
Hpy8I GTNNAC 4 cut(s) 199, 589, 604, 692
Hpy99I CGWCG 5 cut(s) 260, 458, 505, 625, 710
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 1 cut(s) 710
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 2 cut(s) 596, 613
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 64, 145, 148, 151, 538, 622, 631
HpySE526I ACGT 1 cut(s) 264
Hsp92I GRCGYC 1 cut(s) 264
Hsp92II CATG 2 cut(s) 250, 274
HspAI GCGC 3 cut(s) 24, 178, 370
KroI GCCGGC 1 cut(s) 544
KroNI GCCGGC 1 cut(s) 546
Kzo9I GATC 3 cut(s) 243, 273, 459
LguI GCTCTTC 1 cut(s) 323
LmnI GCTCC 5 cut(s) 89, 159, 347, 354, 684
Lsp1109I GCAGC 9 cut(s) 151, 154, 157, 160, 163, 207, 388, 553, 625
LweI GCATC 1 cut(s) 243
MaeI CTAG 1 cut(s) 507
MaeII ACGT 1 cut(s) 264
MalI GATC 3 cut(s) 245, 275, 461
MbiI CCGCTC 1 cut(s) 342
MboI GATC 3 cut(s) 243, 273, 459
MboII GAAGA 3 cut(s) 253, 289, 310
MhlI GDGCHC 4 cut(s) 318, 333, 450, 483
MroNI GCCGGC 1 cut(s) 544
MseI TTAA 1 cut(s) 718
MspA1I CMGCKG 2 cut(s) 220, 401
MspI CCGG 4 cut(s) 21, 373, 545, 558
MspR9I CCNGG 2 cut(s) 346, 558
MvaI CCWGG 1 cut(s) 346
MvnI CGCG 1 cut(s) 453
MwoI GCNNNNNNNGC 8 cut(s) 44, 64, 145, 148, 151, 538, 622, 631
NaeI GCCGGC 1 cut(s) 546
NciI CCSGG 1 cut(s) 558
NdeII GATC 3 cut(s) 243, 273, 459
NgoMIV GCCGGC 1 cut(s) 544
NlaIII CATG 2 cut(s) 250, 274
NlaIV GGNNCC 7 cut(s) 6, 288, 350, 377, 516, 556, 591
NmeAIII GCCGAG 2 cut(s) 307, 382
PaeR7I CTCGAG 1 cut(s) 326
PagI TCATGA 1 cut(s) 246
PceI AGGCCT 1 cut(s) 580
PciSI GCTCTTC 1 cut(s) 323
PdiI GCCGGC 1 cut(s) 546
Pfl23II CGTACG 1 cut(s) 669
Psp124BI GAGCTC 2 cut(s) 318, 333
Psp6I CCWGG 1 cut(s) 344
PspFI CCCAGC 1 cut(s) 54
PspGI CCWGG 1 cut(s) 344
PspLI CGTACG 1 cut(s) 669
PspN4I GGNNCC 7 cut(s) 6, 288, 350, 377, 516, 556, 591
PspPI GGNCC 6 cut(s) 4, 168, 286, 375, 554, 589
PspXI VCTCGAGB 1 cut(s) 326
PstI CTGCAG 1 cut(s) 598
PvuII CAGCTG 1 cut(s) 220
RsaI GTAC 2 cut(s) 521, 671
RsaNI GTAC 2 cut(s) 520, 670
SacI GAGCTC 2 cut(s) 318, 333
SapI GCTCTTC 1 cut(s) 323
SaqAI TTAA 1 cut(s) 718
Sau3AI GATC 3 cut(s) 243, 273, 459
Sau96I GGNCC 6 cut(s) 4, 168, 286, 375, 554, 589
SbfI CCTGCAGG 1 cut(s) 598
ScrFI CCNGG 2 cut(s) 346, 558
SdaI CCTGCAGG 1 cut(s) 598
SduI GDGCHC 4 cut(s) 318, 333, 450, 483
SetI ASST 9 cut(s) 35, 132, 156, 173, 222, 267, 318, 333, 705
SfaNI GCATC 1 cut(s) 243
SfcI CTRYAG 1 cut(s) 594
Sfr274I CTCGAG 1 cut(s) 326
SinI GGWCC 4 cut(s) 4, 168, 375, 589
SlaI CTCGAG 1 cut(s) 326
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
Sse8387I CCTGCAGG 1 cut(s) 598
SseBI AGGCCT 1 cut(s) 580
SspMI CTAG 1 cut(s) 507
SstI GAGCTC 2 cut(s) 318, 333
StuI AGGCCT 1 cut(s) 580
StyD4I CCNGG 2 cut(s) 344, 556
TaaI ACNGT 1 cut(s) 710
TaiI ACGT 1 cut(s) 267
TaqI TCGA 3 cut(s) 327, 426, 705
TauI GCSGC 7 cut(s) 41, 44, 47, 226, 401, 619, 656
Tru1I TTAA 1 cut(s) 718
Tru9I TTAA 1 cut(s) 718
TscAI CASTG 1 cut(s) 694
TseI GCWGC 9 cut(s) 139, 142, 145, 148, 151, 220, 401, 541, 613
TspDTI ATGAA 2 cut(s) 263, 310
TspGWI ACGGA 2 cut(s) 94, 102
TspRI CASTG 1 cut(s) 694
VpaK11BI GGWCC 4 cut(s) 4, 168, 375, 589
XhoI CTCGAG 1 cut(s) 326
XspI CTAG 1 cut(s) 507
ZraI GACGTC 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.