MD14G1168600.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013668
Physical Location & Seq
Reverse (-)
26176740 .. 26178419
1680 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1168600.v1.1.491

Sequence Viewer

Length: 1515 bp
ATGGCTCTTCTTCAATTGCTAAAGGAACCCTCTTTGCTTCTTGCATTCAGTTTCCTCATATTGTTGCTCAAGTTTTTCTTTAAGGATAAGTCAGGGAAAAGAAAACTCAAACTTCCACCAAGCCCTCCGAAGCTGCCTGTCATCGGTAACCTTCACCAGCTCAGAAACAAACCTCACATATCTCTTTGCGGCTTGGCAGAGAAGTATGGTCCACTAATTTACTTACAACTTGGTGAGGTCCCTACTGTGGTGGTTTCATCGGCTAGAGTAGCCAAGGAAGTACTGAAAACCCATGACCTTGCGCTATCAAGCCGTCCACAAATCTTTTCTGCCAAACACCTCTTCTATAATTGTACAGATGTTGCCTTCTCCCCTTACAGCGCTTATTGGAGGCATATCCGTAAAATTTGCATACTTGAGCTACTAAGTGCCAAACGTGTGCAATCTTATAGCCATGTTAGAGAAGAAGAAGTTGCTCGTCTGGTTTGTCGGGTTGCAGAATCTTATCCCGGCACCACCAATCTGAGCAAGATACTTGGATTGTATGCAAATGATGTTCTCTGTCGAGTAGCATTTGGAAGGGGTTTTTCAGAAGGTGGAGACTATGATCGGCATGGGTTCCAAAAGATGCTCGAGGAATACCAGGAATTGCTTGGAGGATTTTCTATTGGAGATTTCTTCCCTTCCATGGAGTTCATACACAGCTTAACAGGAATGAAATCGAGACTTCAAGAGACCTTCCGGCGCTTTGATGAACTTTTCGACCAGATGGTGACTGATCATCTCAATCCCAACAGAGAAAAAGAGAAGCACAAGGATCTCGTAGATGTTTTACTTGACATACAGAAAAGTGAATCTGCTGAAATACCTCTCACCATGGATAATGTCAAGGCTATTATCTTGGACATGTTTGCTGCAGGAACTGACACAACTTTCATCACCCTTGATTGGGGAATGGCAGAGCTTTTGATGAACCCCAAAGTACTGGGAAGAGCGCAAGCAGAAGTACGAGGAATTGTTGGAGAAAGAAGGGTCGTGTTAGAGAGTGATCTGCCTCAGCTGGACTACCTGAAAGCTGTCATCAAAGAGATATTTAGATTGCATCCACCTGCTCCAGTACTACTCCCTAGAGAATCCATGGAAGATGTAAGCATTGATGGGTATGATATTCCAGCCAAAACAAGAATTTTTGTCAATGCCTGGGCAATAGGGAGGGACCCAGAATCTTGGGAAAATCCAGAAACGTTTGAACCAGAAAGATTCATAGGTAGCACTATAGACTTCAAGGGGCAGCATTATGAGCTTTTACCTTTTGGGGCTGGTAGAAGAATTTGTCCAGCTATTACGTTTGGAACAGCAACCACTGAGCTTGCTCTAGCTCAACTTCTGCATAGCTTTGATTGGGCGCTTCCTCCGGGTACTACATCTAAAGATTTGGACCTGGCAGAAGTTTTTGGTATCACAATGCACAGGATAGCCAATCTGTTTGTTGTCGCCAGACCACGGTTTCCCTGA

Protein Analysis

505

Amino Acids

57.02

Weight (kDa)

6.72

Isoelectric Point (pI)

44.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 39 - 487 9e-104 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1117
Acc36I ACCTGC 1 cut(s) 1117
AccB1I GGYRCC 1 cut(s) 512
AciI CCGC 1 cut(s) 189
AclI AACGTT 1 cut(s) 1244
AclWI GGATC 1 cut(s) 825
AcsI RAATTY 3 cut(s) 405, 1185, 1329
AfaI GTAC 6 cut(s) 282, 355, 984, 1008, 1119, 1420
AfeI AGCGCT 1 cut(s) 382
AfiI CCNNNNNNNGG 6 cut(s) 143, 247, 688, 948, 949, 1503
AflIII ACRYGT 2 cut(s) 436, 906
AgsI TTSAA 4 cut(s) 14, 731, 1250, 1285
AjnI CCWGG 3 cut(s) 642, 1199, 1440
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
Alw26I GTCTC 3 cut(s) 594, 718, 728
AlwI GGATC 1 cut(s) 825
AlwNI CAGNNNCTG 1 cut(s) 923
Ama87I CYCGRG 1 cut(s) 632
Aor51HI AGCGCT 1 cut(s) 382
ApeKI GCWGC 3 cut(s) 133, 914, 1291
ApoI RAATTY 3 cut(s) 405, 1185, 1329
AspLEI GCGC 5 cut(s) 304, 383, 747, 997, 1408
AspS9I GGNCC 4 cut(s) 209, 238, 1216, 1438
AsuC2I CCSGG 2 cut(s) 510, 1416
AsuHPI GGTGA 5 cut(s) 146, 245, 784, 865, 931
AvaI CYCGRG 1 cut(s) 632
AvaII GGWCC 4 cut(s) 209, 238, 1216, 1438
BanI GGYRCC 1 cut(s) 512
BbvCI CCTCAGC 1 cut(s) 1056
BbvI GCAGC 3 cut(s) 120, 901, 1303
BccI CCATC 2 cut(s) 763, 1151
BceAI ACGGC 1 cut(s) 297
BciT130I CCWGG 3 cut(s) 644, 1201, 1442
BclI TGATCA 1 cut(s) 778
BcnI CCSGG 2 cut(s) 510, 1416
BcoDI GTCTC 3 cut(s) 594, 718, 728
BfaI CTAG 3 cut(s) 264, 1128, 1376
BfmI CTRYAG 2 cut(s) 915, 1275
BfoI RGCGCY 3 cut(s) 384, 748, 1409
BfuAI ACCTGC 1 cut(s) 1117
BisI GCNGC 4 cut(s) 134, 190, 915, 1292
BlsI GCNGC 4 cut(s) 135, 191, 916, 1293
BmcAI AGTACT 3 cut(s) 282, 984, 1119
Bme1390I CCNGG 5 cut(s) 510, 644, 1201, 1416, 1442
Bme18I GGWCC 4 cut(s) 209, 238, 1216, 1438
BmeT110I CYCGRG 1 cut(s) 632
BmgT120I GGNCC 4 cut(s) 209, 238, 1216, 1438
BmiI GGNNCC 6 cut(s) 27, 240, 514, 620, 1217, 1218
BmrFI CCNGG 5 cut(s) 510, 644, 1201, 1416, 1442
BmrI ACTGGG 1 cut(s) 995
BmsI GCATC 2 cut(s) 618, 1111
BmuI ACTGGG 1 cut(s) 995
BpmI CTGGAG 1 cut(s) 1098
Bpu10I CCTNAGC 1 cut(s) 1056
BpuEI CTTGAG 2 cut(s) 53, 437
BpuMI CCSGG 2 cut(s) 510, 1416
BsaI GGTCTC 1 cut(s) 728
BsaJI CCNNGG 6 cut(s) 273, 687, 876, 1137, 1200, 1502
Bsc4I CCNNNNNNNGG 6 cut(s) 143, 247, 688, 948, 949, 1503
Bse1I ACTGG 2 cut(s) 990, 1115
BseBI CCWGG 3 cut(s) 644, 1201, 1442
BseDI CCNNGG 6 cut(s) 273, 687, 876, 1137, 1200, 1502
BseGI GGATG 1 cut(s) 1102
BseLI CCNNNNNNNGG 6 cut(s) 143, 247, 688, 948, 949, 1503
BseMII CTCAG 4 cut(s) 175, 515, 1070, 1356
BseNI ACTGG 2 cut(s) 990, 1115
BseXI GCAGC 3 cut(s) 120, 901, 1303
BshNI GGYRCC 1 cut(s) 512
BsiHKCI CYCGRG 1 cut(s) 632
BsiSI CCGG 3 cut(s) 510, 742, 1415
BslFI GGGAC 2 cut(s) 224, 1229
BslI CCNNNNNNNGG 6 cut(s) 143, 247, 688, 948, 949, 1503
BsmAI GTCTC 3 cut(s) 594, 718, 728
BsmFI GGGAC 2 cut(s) 224, 1229
BsmI GAATGC 1 cut(s) 44
Bso31I GGTCTC 1 cut(s) 728
BsoBI CYCGRG 1 cut(s) 632
Bsp1407I TGTACA 1 cut(s) 353
Bsp143I GATC 4 cut(s) 607, 778, 817, 1048
Bsp19I CCATGG 3 cut(s) 687, 876, 1137
BspACI CCGC 1 cut(s) 189
BspCNI CTCAG 4 cut(s) 174, 516, 1069, 1357
BspLI GGNNCC 6 cut(s) 27, 240, 514, 620, 1217, 1218
BspMAI CTGCAG 1 cut(s) 919
BspMI ACCTGC 1 cut(s) 1117
BspPI GGATC 1 cut(s) 825
BspQI GCTCTTC 2 cut(s) 12, 985
BspT107I GGYRCC 1 cut(s) 512
BspTNI GGTCTC 1 cut(s) 728
BsrGI TGTACA 1 cut(s) 353
BsrI ACTGG 2 cut(s) 990, 1115
BssECI CCNNGG 6 cut(s) 273, 687, 876, 1137, 1200, 1502
BssMI GATC 4 cut(s) 607, 778, 817, 1048
BssT1I CCWWGG 4 cut(s) 273, 687, 876, 1137
Bst2UI CCWGG 3 cut(s) 644, 1201, 1442
Bst4CI ACNGT 2 cut(s) 247, 1506
Bst6I CTCTTC 3 cut(s) 12, 347, 985
BstAUI TGTACA 1 cut(s) 353
BstC8I GCNNGC 2 cut(s) 999, 1371
BstDEI CTNAG 5 cut(s) 161, 425, 524, 1056, 1365
BstDSI CCRYGG 4 cut(s) 687, 876, 1137, 1502
BstEII GGTNACC 1 cut(s) 146
BstF5I GGATG 1 cut(s) 1102
BstH2I RGCGCY 3 cut(s) 384, 748, 1409
BstHHI GCGC 5 cut(s) 304, 383, 747, 997, 1408
BstKTI GATC 4 cut(s) 610, 781, 820, 1051
BstMAI GTCTC 3 cut(s) 594, 718, 728
BstMBI GATC 4 cut(s) 607, 778, 817, 1048
BstMWI GCNNNNNNNGC 2 cut(s) 269, 1300
BstNI CCWGG 3 cut(s) 644, 1201, 1442
BstNSI RCATGY 1 cut(s) 910
BstPI GGTNACC 1 cut(s) 146
BstSCI CCNGG 5 cut(s) 508, 642, 1199, 1414, 1440
BstSFI CTRYAG 2 cut(s) 915, 1275
BstV1I GCAGC 3 cut(s) 120, 901, 1303
BstX2I RGATCY 1 cut(s) 817
BstXI CCANNNNNNTGG 2 cut(s) 985, 1227
BstYI RGATCY 1 cut(s) 817
BtgI CCRYGG 4 cut(s) 687, 876, 1137, 1502
BtsCI GGATG 1 cut(s) 1102
BtsIMutI CAGTG 1 cut(s) 1362
BveI ACCTGC 1 cut(s) 1117
Cac8I GCNNGC 2 cut(s) 999, 1371
CaiI CAGNNNCTG 1 cut(s) 923
CfoI GCGC 5 cut(s) 304, 383, 747, 997, 1408
Cfr13I GGNCC 4 cut(s) 209, 238, 1216, 1438
Csp6I GTAC 6 cut(s) 281, 354, 983, 1007, 1118, 1419
CspCI CAANNNNNGTGG 2 cut(s) 1351, 1386
CviAII CATG 7 cut(s) 293, 455, 614, 688, 877, 907, 1138
CviQI GTAC 6 cut(s) 281, 354, 983, 1007, 1118, 1419
DdeI CTNAG 5 cut(s) 161, 425, 524, 1056, 1365
DpnI GATC 4 cut(s) 609, 780, 819, 1050
DpnII GATC 4 cut(s) 607, 778, 817, 1048
Eam1104I CTCTTC 3 cut(s) 12, 347, 985
EarI CTCTTC 3 cut(s) 12, 347, 985
Eco130I CCWWGG 4 cut(s) 273, 687, 876, 1137
Eco31I GGTCTC 1 cut(s) 728
Eco47I GGWCC 4 cut(s) 209, 238, 1216, 1438
Eco47III AGCGCT 1 cut(s) 382
Eco88I CYCGRG 1 cut(s) 632
Eco91I GGTNACC 1 cut(s) 146
EcoO109I RGGNCCY 2 cut(s) 238, 1216
EcoO65I GGTNACC 1 cut(s) 146
EcoRII CCWGG 3 cut(s) 642, 1199, 1440
EcoT14I CCWWGG 4 cut(s) 273, 687, 876, 1137
ErhI CCWWGG 4 cut(s) 273, 687, 876, 1137
FaeI CATG 7 cut(s) 296, 458, 617, 691, 880, 910, 1141
FalI AAGNNNNNCTT 2 cut(s) 62, 94
FaqI GGGAC 2 cut(s) 224, 1229
FatI CATG 7 cut(s) 292, 454, 613, 687, 876, 906, 1137
FbaI TGATCA 1 cut(s) 778
Fnu4HI GCNGC 4 cut(s) 134, 190, 915, 1292
FokI GGATG 1 cut(s) 1089
Fsp4HI GCNGC 4 cut(s) 134, 190, 915, 1292
FspBI CTAG 3 cut(s) 264, 1128, 1376
GlaI GCGC 5 cut(s) 303, 382, 746, 996, 1407
GluI GCNGC 4 cut(s) 134, 190, 915, 1292
GsuI CTGGAG 1 cut(s) 1098
HaeII RGCGCY 3 cut(s) 384, 748, 1409
HapII CCGG 3 cut(s) 510, 742, 1415
HhaI GCGC 5 cut(s) 304, 383, 747, 997, 1408
Hin1II CATG 7 cut(s) 296, 458, 617, 691, 880, 910, 1141
Hin6I GCGC 5 cut(s) 302, 381, 745, 995, 1406
HinP1I GCGC 5 cut(s) 302, 381, 745, 995, 1406
HinfI GANTC 5 cut(s) 500, 854, 1133, 1223, 1260
HpaII CCGG 3 cut(s) 510, 742, 1415
HphI GGTGA 5 cut(s) 146, 245, 784, 865, 931
Hpy166II GTNNAC 2 cut(s) 212, 317
Hpy188I TCNGA 4 cut(s) 129, 164, 525, 592
Hpy188III TCNNGA 3 cut(s) 723, 731, 1238
Hpy8I GTNNAC 2 cut(s) 212, 317
HpyAV CCTTC 7 cut(s) 161, 376, 573, 587, 693, 748, 1023
HpyCH4III ACNGT 2 cut(s) 247, 1506
HpyCH4IV ACGT 3 cut(s) 436, 1244, 1346
HpyCH4V TGCA 9 cut(s) 44, 411, 442, 497, 548, 917, 1102, 1390, 1468
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 1300
HpyF3I CTNAG 5 cut(s) 161, 425, 524, 1056, 1365
HpySE526I ACGT 3 cut(s) 436, 1244, 1346
Hsp92II CATG 7 cut(s) 296, 458, 617, 691, 880, 910, 1141
HspAI GCGC 5 cut(s) 302, 381, 745, 995, 1406
KflI GGGWCCC 1 cut(s) 1216
Ksp22I TGATCA 1 cut(s) 778
Kzo9I GATC 4 cut(s) 607, 778, 817, 1048
LguI GCTCTTC 2 cut(s) 12, 985
LmnI GCTCC 1 cut(s) 1117
Lsp1109I GCAGC 3 cut(s) 120, 901, 1303
LweI GCATC 2 cut(s) 618, 1111
MaeI CTAG 3 cut(s) 264, 1128, 1376
MaeII ACGT 3 cut(s) 436, 1244, 1346
MaeIII GTNAC 2 cut(s) 146, 772
MalI GATC 4 cut(s) 609, 780, 819, 1050
MboI GATC 4 cut(s) 607, 778, 817, 1048
MboII GAAGA 7 cut(s) 334, 476, 479, 670, 1002, 1154, 1338
MfeI CAATTG 1 cut(s) 14
MflI RGATCY 1 cut(s) 817
MluCI AATT 8 cut(s) 14, 216, 349, 405, 647, 1014, 1185, 1329
MmeI TCCRAC 1 cut(s) 1000
MseI TTAA 2 cut(s) 81, 707
MspA1I CMGCKG 1 cut(s) 1060
MspI CCGG 3 cut(s) 510, 742, 1415
MspR9I CCNGG 5 cut(s) 510, 644, 1201, 1416, 1442
MunI CAATTG 1 cut(s) 14
Mva1269I GAATGC 1 cut(s) 44
MvaI CCWGG 3 cut(s) 644, 1201, 1442
MwoI GCNNNNNNNGC 2 cut(s) 269, 1300
NciI CCSGG 2 cut(s) 510, 1416
NcoI CCATGG 3 cut(s) 687, 876, 1137
NdeII GATC 4 cut(s) 607, 778, 817, 1048
NlaIII CATG 7 cut(s) 296, 458, 617, 691, 880, 910, 1141
NlaIV GGNNCC 6 cut(s) 27, 240, 514, 620, 1217, 1218
NmuCI GTSAC 1 cut(s) 772
NspI RCATGY 1 cut(s) 910
PaeR7I CTCGAG 1 cut(s) 632
PaqCI CACCTGC 1 cut(s) 1117
PciI ACATGT 1 cut(s) 906
PciSI GCTCTTC 2 cut(s) 12, 985
PctI GAATGC 1 cut(s) 44
PfeI GAWTC 5 cut(s) 500, 854, 1133, 1223, 1260
PkrI GCNGC 4 cut(s) 135, 191, 916, 1293
PpuMI RGGWCCY 2 cut(s) 238, 1216
PscI ACATGT 1 cut(s) 906
Psp1406I AACGTT 1 cut(s) 1244
Psp5II RGGWCCY 2 cut(s) 238, 1216
Psp6I CCWGG 3 cut(s) 642, 1199, 1440
PspEI GGTNACC 1 cut(s) 146
PspGI CCWGG 3 cut(s) 642, 1199, 1440
PspN4I GGNNCC 6 cut(s) 27, 240, 514, 620, 1217, 1218
PspPI GGNCC 4 cut(s) 209, 238, 1216, 1438
PspPPI RGGWCCY 2 cut(s) 238, 1216
PspXI VCTCGAGB 1 cut(s) 632
PstI CTGCAG 1 cut(s) 919
PstNI CAGNNNCTG 1 cut(s) 923
PsuI RGATCY 1 cut(s) 817
PvuII CAGCTG 1 cut(s) 1060
RsaI GTAC 6 cut(s) 282, 355, 984, 1008, 1119, 1420
RsaNI GTAC 6 cut(s) 281, 354, 983, 1007, 1118, 1419
SapI GCTCTTC 2 cut(s) 12, 985
SaqAI TTAA 2 cut(s) 81, 707
SatI GCNGC 4 cut(s) 134, 190, 915, 1292
Sau3AI GATC 4 cut(s) 607, 778, 817, 1048
Sau96I GGNCC 4 cut(s) 209, 238, 1216, 1438
ScaI AGTACT 3 cut(s) 282, 984, 1119
ScrFI CCNGG 5 cut(s) 510, 644, 1201, 1416, 1442
SfaNI GCATC 2 cut(s) 618, 1111
SfcI CTRYAG 2 cut(s) 915, 1275
Sfr274I CTCGAG 1 cut(s) 632
SinI GGWCC 4 cut(s) 209, 238, 1216, 1438
SlaI CTCGAG 1 cut(s) 632
SmlI CTYRAG 3 cut(s) 68, 416, 632
SmoI CTYRAG 3 cut(s) 68, 416, 632
Sse9I AATT 8 cut(s) 14, 216, 349, 405, 647, 1014, 1185, 1329
SsiI CCGC 1 cut(s) 189
SspMI CTAG 3 cut(s) 264, 1128, 1376
StyD4I CCNGG 5 cut(s) 508, 642, 1199, 1414, 1440
StyI CCWWGG 4 cut(s) 273, 687, 876, 1137
TaaI ACNGT 2 cut(s) 247, 1506
TaiI ACGT 3 cut(s) 439, 1247, 1349
TaqI TCGA 4 cut(s) 565, 633, 722, 762
TasI AATT 8 cut(s) 14, 216, 349, 405, 647, 1014, 1185, 1329
TatI WGTACW 4 cut(s) 280, 353, 982, 1117
TauI GCSGC 1 cut(s) 192
TfiI GAWTC 5 cut(s) 500, 854, 1133, 1223, 1260
Tru1I TTAA 2 cut(s) 81, 707
Tru9I TTAA 2 cut(s) 81, 707
TscAI CASTG 1 cut(s) 1369
TseFI GTSAC 1 cut(s) 772
TseI GCWGC 3 cut(s) 133, 914, 1291
Tsp45I GTSAC 1 cut(s) 772
TspDTI ATGAA 7 cut(s) 246, 685, 731, 768, 925, 986, 1252
TspGWI ACGGA 1 cut(s) 389
TspRI CASTG 1 cut(s) 1369
VpaK11BI GGWCC 4 cut(s) 209, 238, 1216, 1438
XapI RAATTY 3 cut(s) 405, 1185, 1329
XceI RCATGY 1 cut(s) 910
XcmI CCANNNNNNNNNTGG 1 cut(s) 650
XhoI CTCGAG 1 cut(s) 632
XspI CTAG 3 cut(s) 264, 1128, 1376
ZrmI AGTACT 3 cut(s) 282, 984, 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.