Rroxscaffold_3G00263250

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013668
Physical Location & Seq
Forward (+)
57222654 .. 57223879
1226 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00263250.1

Sequence Viewer

Length: 1098 bp
ATGGCTCTTCTTCAATTTCTAAAGGAACCTTCTGTGCTTTTTGCATTCAGTTTTCTCATAGTGTTGCTCAAATATATCTTCAGGGATAAGTTGAGGAAAACAAAAATCAAACTCCCACCTAGCCCTCCAAAGCTACCCATCATTGGTAACCTTCACCAGCTTGGAAACAGCCCTCACCTATCTCTCTGGTCCTTGGCAGAAAAGTCTGGTCCCTTAATTTACTTACAACTTGGTCACATCCCAACAGTGGTGGTGTCATCAGCTAAACTAGCCAGGGAAGTACTGAAAACTCATGACCTTGCACTTTCTAGCAACCCACAGATCTTTTCAGCCAAACATCTCTTCTATAACTGCACTGATGTTGCCTTCTCCCCTTATGGTGCTGATTGGAGGCACATTCGTAAGATTTGCATACGTGAGCTACTAAGTGCCAAAAGAGTGCAATCATTTAGTCATGTAAGAGTAGAAAAAGTTGCTCGTCTGGCTCATCGGGTTGCAGAGTCCTATCCGAGCACCACTAATCTGAGCAAGCTGCTTGGACTATATGCAAATGATATTCTCTGCCGCGTAGCATTTGGAAGAGATTTCTCAGGTGGTGGAGAGTATGATCAGCATGGTTTCCAAAAGATGCTTGAGAAATACCAGGAATTGCTTGGCGGATTTTCTATTGGAGATTTCTTCCCTTCTATGGAGTTTCTGCACACCTTAACAGGAATGAAATCAAGACTTCAAGAGACATTCGGGAGATTCGATAAACTATTTGACCAGATGATAACTGAACATCAAAATCCCAAAAGAGAAAAACAGGAGCACATGGACCTCGTGGATGTTTTACTGGATATACAGAAAAAAGATTCTGCTGACATGTCTCTCACCATGGATAATGTCAAGGCTAGTATCTTGGACATGTTTGCTGCAGGAACTGACACAACCTTCATCACCCTTGATTGGGGAATGACAGAGCTTTTGATGAACCCCAAAGTCCTGGAAAAAGCACAAGCTGAAGTACGAGGTGTTGTTGGGGAAAGAAGAGTCGTATTCGAGAGTGATCTGCATCAGCTGGACTACATGAAAGCTGTCATCAAAGAGGATCTATAG

Protein Analysis

365

Amino Acids

41.52

Weight (kDa)

8.75

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 39 - 363 5.3e-61 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 567
AciI CCGC 2 cut(s) 565, 657
AcuI CTGAAG 2 cut(s) 64, 1023
AfaI GTAC 2 cut(s) 282, 1008
AfiI CCNNNNNNNGG 5 cut(s) 143, 247, 688, 948, 949
AflIII ACRYGT 2 cut(s) 864, 906
AgsI TTSAA 2 cut(s) 14, 731
AjnI CCWGG 3 cut(s) 272, 642, 984
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AluBI AGCT 9 cut(s) 133, 160, 263, 421, 532, 964, 1001, 1060, 1076
AluI AGCT 9 cut(s) 133, 160, 263, 421, 532, 964, 1001, 1060, 1076
Alw21I GWGCWC 2 cut(s) 515, 813
Alw26I GTCTC 2 cut(s) 728, 873
AlwNI CAGNNNCTG 1 cut(s) 923
ApeKI GCWGC 2 cut(s) 532, 914
ArsI GACNNNNNNTTYG 2 cut(s) 949, 981
AspS9I GGNCC 3 cut(s) 189, 209, 817
AsuHPI GGTGA 4 cut(s) 146, 167, 865, 931
AvaII GGWCC 3 cut(s) 189, 209, 817
BauI CACGAG 1 cut(s) 821
Bbv12I GWGCWC 2 cut(s) 515, 813
BbvI GCAGC 2 cut(s) 519, 901
BccI CCATC 1 cut(s) 146
BciT130I CCWGG 3 cut(s) 274, 644, 986
BclI TGATCA 1 cut(s) 607
BcoDI GTCTC 2 cut(s) 728, 873
BfaI CTAG 4 cut(s) 120, 269, 309, 894
BfmI CTRYAG 2 cut(s) 915, 1094
BglII AGATCT 1 cut(s) 321
BisI GCNGC 3 cut(s) 533, 565, 915
BlsI GCNGC 3 cut(s) 534, 566, 916
BmcAI AGTACT 1 cut(s) 282
Bme1390I CCNGG 3 cut(s) 274, 644, 986
Bme18I GGWCC 3 cut(s) 189, 209, 817
BmgT120I GGNCC 3 cut(s) 189, 209, 817
BmiI GGNNCC 2 cut(s) 27, 211
BmrFI CCNGG 3 cut(s) 274, 644, 986
BmsI GCATC 2 cut(s) 618, 1063
BpuEI CTTGAG 1 cut(s) 653
BsaAI YACGTR 1 cut(s) 416
BsaBI GATNNNNATC 1 cut(s) 1053
BsaJI CCNNGG 3 cut(s) 192, 273, 876
Bsc4I CCNNNNNNNGG 5 cut(s) 143, 247, 688, 948, 949
Bse1I ACTGG 1 cut(s) 840
Bse8I GATNNNNATC 1 cut(s) 1053
BseBI CCWGG 3 cut(s) 274, 644, 986
BseDI CCNNGG 3 cut(s) 192, 273, 876
BseGI GGATG 2 cut(s) 237, 832
BseJI GATNNNNATC 1 cut(s) 1053
BseLI CCNNNNNNNGG 5 cut(s) 143, 247, 688, 948, 949
BseMII CTCAG 2 cut(s) 515, 603
BseNI ACTGG 1 cut(s) 840
BseXI GCAGC 2 cut(s) 519, 901
BsgI GTGCAG 2 cut(s) 337, 683
Bsh1236I CGCG 1 cut(s) 567
BsiHKAI GWGCWC 2 cut(s) 515, 813
BslFI GGGAC 1 cut(s) 195
BslI CCNNNNNNNGG 5 cut(s) 143, 247, 688, 948, 949
BsmAI GTCTC 2 cut(s) 728, 873
BsmFI GGGAC 1 cut(s) 195
BsmI GAATGC 1 cut(s) 44
Bsp1286I GDGCHC 2 cut(s) 515, 813
Bsp143I GATC 4 cut(s) 321, 607, 1048, 1090
Bsp19I CCATGG 1 cut(s) 876
BspACI CCGC 2 cut(s) 565, 657
BspCNI CTCAG 2 cut(s) 516, 602
BspFNI CGCG 1 cut(s) 567
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 2 cut(s) 27, 211
BspMAI CTGCAG 1 cut(s) 919
BspQI GCTCTTC 1 cut(s) 12
BsrI ACTGG 1 cut(s) 840
BssECI CCNNGG 3 cut(s) 192, 273, 876
BssMI GATC 4 cut(s) 321, 607, 1048, 1090
BssSI CACGAG 1 cut(s) 821
BssT1I CCWWGG 2 cut(s) 192, 876
Bst2BI CACGAG 1 cut(s) 821
Bst2UI CCWGG 3 cut(s) 274, 644, 986
Bst4CI ACNGT 1 cut(s) 247
Bst6I CTCTTC 4 cut(s) 12, 347, 574, 1024
BstBAI YACGTR 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 530
BstDEI CTNAG 3 cut(s) 425, 524, 589
BstDSI CCRYGG 1 cut(s) 876
BstEII GGTNACC 1 cut(s) 146
BstF5I GGATG 2 cut(s) 237, 832
BstFNI CGCG 1 cut(s) 567
BstKTI GATC 4 cut(s) 324, 610, 1051, 1093
BstMAI GTCTC 2 cut(s) 728, 873
BstMBI GATC 4 cut(s) 321, 607, 1048, 1090
BstMWI GCNNNNNNNGC 2 cut(s) 269, 482
BstNI CCWGG 3 cut(s) 274, 644, 986
BstNSI RCATGY 2 cut(s) 868, 910
BstPI GGTNACC 1 cut(s) 146
BstSCI CCNGG 3 cut(s) 272, 642, 984
BstSFI CTRYAG 2 cut(s) 915, 1094
BstUI CGCG 1 cut(s) 567
BstV1I GCAGC 2 cut(s) 519, 901
BstX2I RGATCY 2 cut(s) 321, 1090
BstXI CCANNNNNNTGG 1 cut(s) 985
BstYI RGATCY 2 cut(s) 321, 1090
BtgI CCRYGG 1 cut(s) 876
BtsCI GGATG 2 cut(s) 237, 832
BtsIMutI CAGTG 2 cut(s) 252, 354
Cac8I GCNNGC 1 cut(s) 530
CaiI CAGNNNCTG 1 cut(s) 923
CciI TCATGA 1 cut(s) 292
Cfr13I GGNCC 3 cut(s) 189, 209, 817
Csp6I GTAC 2 cut(s) 281, 1007
CspCI CAANNNNNGTGG 2 cut(s) 231, 266
CviAII CATG 8 cut(s) 293, 455, 614, 814, 865, 877, 907, 1069
CviQI GTAC 2 cut(s) 281, 1007
DdeI CTNAG 3 cut(s) 425, 524, 589
DpnI GATC 4 cut(s) 323, 609, 1050, 1092
DpnII GATC 4 cut(s) 321, 607, 1048, 1090
Eam1104I CTCTTC 4 cut(s) 12, 347, 574, 1024
EarI CTCTTC 4 cut(s) 12, 347, 574, 1024
EciI GGCGGA 1 cut(s) 672
Eco130I CCWWGG 2 cut(s) 192, 876
Eco47I GGWCC 3 cut(s) 189, 209, 817
Eco57I CTGAAG 2 cut(s) 64, 1023
Eco91I GGTNACC 1 cut(s) 146
EcoO65I GGTNACC 1 cut(s) 146
EcoRII CCWGG 3 cut(s) 272, 642, 984
EcoT14I CCWWGG 2 cut(s) 192, 876
ErhI CCWWGG 2 cut(s) 192, 876
FaeI CATG 8 cut(s) 296, 458, 617, 817, 868, 880, 910, 1072
FaqI GGGAC 1 cut(s) 195
FatI CATG 8 cut(s) 292, 454, 613, 813, 864, 876, 906, 1068
FbaI TGATCA 1 cut(s) 607
Fnu4HI GCNGC 3 cut(s) 533, 565, 915
FokI GGATG 2 cut(s) 224, 839
Fsp4HI GCNGC 3 cut(s) 533, 565, 915
FspBI CTAG 4 cut(s) 120, 269, 309, 894
GluI GCNGC 3 cut(s) 533, 565, 915
Hin1II CATG 8 cut(s) 296, 458, 617, 817, 868, 880, 910, 1072
HinfI GANTC 4 cut(s) 500, 747, 854, 1032
HphI GGTGA 4 cut(s) 146, 167, 865, 931
Hpy188I TCNGA 2 cut(s) 510, 525
Hpy188III TCNNGA 5 cut(s) 293, 723, 731, 742, 1042
HpyAV CCTTC 5 cut(s) 39, 161, 376, 693, 943
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 1 cut(s) 415
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 482
HpyF3I CTNAG 3 cut(s) 425, 524, 589
HpySE526I ACGT 1 cut(s) 415
Hsp92II CATG 8 cut(s) 296, 458, 617, 817, 868, 880, 910, 1072
Ksp22I TGATCA 1 cut(s) 607
Kzo9I GATC 4 cut(s) 321, 607, 1048, 1090
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 808
Lsp1109I GCAGC 2 cut(s) 519, 901
LweI GCATC 2 cut(s) 618, 1063
MaeI CTAG 4 cut(s) 120, 269, 309, 894
MaeII ACGT 1 cut(s) 415
MaeIII GTNAC 2 cut(s) 146, 233
MalI GATC 4 cut(s) 323, 609, 1050, 1092
MboI GATC 4 cut(s) 321, 607, 1048, 1090
MboII GAAGA 5 cut(s) 70, 334, 591, 670, 1041
MflI RGATCY 2 cut(s) 321, 1090
MhlI GDGCHC 2 cut(s) 515, 813
MluCI AATT 3 cut(s) 14, 216, 647
MlyI GAGTC 2 cut(s) 509, 1041
MnlI CCTC 7 cut(s) 87, 135, 183, 384, 830, 1004, 1081
MseI TTAA 2 cut(s) 215, 707
MspA1I CMGCKG 1 cut(s) 1060
MspR9I CCNGG 3 cut(s) 274, 644, 986
Mva1269I GAATGC 1 cut(s) 44
MvaI CCWGG 3 cut(s) 274, 644, 986
MvnI CGCG 1 cut(s) 567
MwoI GCNNNNNNNGC 2 cut(s) 269, 482
NcoI CCATGG 1 cut(s) 876
NdeII GATC 4 cut(s) 321, 607, 1048, 1090
NlaIII CATG 8 cut(s) 296, 458, 617, 817, 868, 880, 910, 1072
NlaIV GGNNCC 2 cut(s) 27, 211
NmuCI GTSAC 1 cut(s) 233
NspI RCATGY 2 cut(s) 868, 910
PagI TCATGA 1 cut(s) 292
PciI ACATGT 2 cut(s) 864, 906
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 747
PctI GAATGC 1 cut(s) 44
PfeI GAWTC 2 cut(s) 747, 854
PfoI TCCNGGA 1 cut(s) 984
PkrI GCNGC 3 cut(s) 534, 566, 916
PleI GAGTC 2 cut(s) 508, 1040
PpsI GAGTC 2 cut(s) 508, 1040
Ppu21I YACGTR 1 cut(s) 416
PscI ACATGT 2 cut(s) 864, 906
Psp6I CCWGG 3 cut(s) 272, 642, 984
PspEI GGTNACC 1 cut(s) 146
PspGI CCWGG 3 cut(s) 272, 642, 984
PspN4I GGNNCC 2 cut(s) 27, 211
PspPI GGNCC 3 cut(s) 189, 209, 817
PstI CTGCAG 1 cut(s) 919
PstNI CAGNNNCTG 1 cut(s) 923
PsuI RGATCY 2 cut(s) 321, 1090
PvuII CAGCTG 1 cut(s) 1060
RsaI GTAC 2 cut(s) 282, 1008
RsaNI GTAC 2 cut(s) 281, 1007
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 215, 707
SatI GCNGC 3 cut(s) 533, 565, 915
Sau3AI GATC 4 cut(s) 321, 607, 1048, 1090
Sau96I GGNCC 3 cut(s) 189, 209, 817
ScaI AGTACT 1 cut(s) 282
SchI GAGTC 2 cut(s) 509, 1041
ScrFI CCNGG 3 cut(s) 274, 644, 986
SduI GDGCHC 2 cut(s) 515, 813
SfaNI GCATC 2 cut(s) 618, 1063
SfcI CTRYAG 2 cut(s) 915, 1094
SinI GGWCC 3 cut(s) 189, 209, 817
SmlI CTYRAG 1 cut(s) 632
SmoI CTYRAG 1 cut(s) 632
Sse9I AATT 3 cut(s) 14, 216, 647
SsiI CCGC 2 cut(s) 565, 657
SspMI CTAG 4 cut(s) 120, 269, 309, 894
StyD4I CCNGG 3 cut(s) 272, 642, 984
StyI CCWWGG 2 cut(s) 192, 876
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 1 cut(s) 418
TaqI TCGA 2 cut(s) 750, 1041
TasI AATT 3 cut(s) 14, 216, 647
TatI WGTACW 1 cut(s) 280
TauI GCSGC 1 cut(s) 567
TfiI GAWTC 2 cut(s) 747, 854
Tru1I TTAA 2 cut(s) 215, 707
Tru9I TTAA 2 cut(s) 215, 707
TscAI CASTG 2 cut(s) 252, 361
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 2 cut(s) 532, 914
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 4 cut(s) 731, 925, 986, 1085
TspRI CASTG 2 cut(s) 252, 361
VpaK11BI GGWCC 3 cut(s) 189, 209, 817
XceI RCATGY 2 cut(s) 868, 910
XcmI CCANNNNNNNNNTGG 1 cut(s) 650
XspI CTAG 4 cut(s) 120, 269, 309, 894
ZrmI AGTACT 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.