Rw7G010570

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0013668
Physical Location & Seq
Reverse (-)
9822577 .. 9824220
1644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G010570.1

Sequence Viewer

Length: 1515 bp
ATGGATCTTCTTCAATTTCTTAACATACCCTCTTTGCTTTTGGCATTCAGTTTCCTCATAGTGTTGCTTACATTCCTCTTGAAGGATAGTTCAACAAAAAGAAAACTTAAACTCCCACCAAGCCCTCCAAAGCTACCTGTTGTTGGCAACCTTCACCAGCTAGGAAACAAGCCTCACCTATCTCTCCGGTCCTTGGCACAAAAGTATGGTCCAATAATTTACTTACAACTTGGTGAGATCCCAACTGTGGTGGTTTCATCAGCTAGACTTGCCAAGGAAGTACTGAAAACCCATGATCTTGCACTATCAAGCCGTCCACAAATCTTTTCAGCTAAACACCTCTTCTATAATTGCACTGATGTTGTCTTCTCCCCTTATGGTGCTTATTGGAGACACATACGTAAGATTTGCATACTTGAGCTGTTAAGTGCCAAAAGAGTGCAGTCATTTAGCCATGCCAGAGAAGAAGAAGTTGCTCGTCTGGCTCGTCGGGTTGCAGAGTCCTATCCCGGCACCACTAATCTCAGCAAGTTGCTTGGACTCTATGCAAATGATATTCTCTGCCGCGTAGCATTTGGAAGAGATTTCTCAGGTGGTGGAGAGTATGATCAGCATGATTTCCAAAAGATGCTTGAGGAATATCAGGAATTGCTAGGTGGATTTTCTATTGGAGATTTCTTCCCTTCCATGGAGTTCCTCCACACCTTAACAGGAATGAAATCAAGACTTCAAGACACATTCAGTCGATTTGATAAACTCTTCGACCAGATTTTGACTGAACATCTCAATCCCAACAGAGAAAAAGAGGAGCATAAGGACCTCGTGGATGTTTTACTGGAGATACAGAAGGAAGAGACTGCCGATATGCCTCTAACCATGGATAATGTCAAGGCTATTATTTTGGACATGTTTGCTGCTGGAACAGACACAACCTTCATCACCCTTGATTGGGGGATGACAGAACTTCTGATGAACCCCAAAGTTCTCAAAAAAGCACAAGCTGAAGTAAGAGGTGTTGTTAGGGAAAAAAGAGTTGTGTTAGAGGGTGATCTTCATCAGCTGGACTATTTGAAAGCTGTCATTAAAGAGATATTTCGGCTGCATCCACCTGCACCAGTACTAGTACCTAGAGAATCCATGGAAGATGTAACCATTGATGGATACGACATTCTAGCCAAAACAAGAATTTTTGTCAATGCCTGGGCAATAGGGAGAGACCCAGAATCTTGGGAAAACCCAGAAAAGTTTGAACCAGAAAGATTTATGGGTAGCACTATTGATTTCAAGGGGCAGGATTTTGAGCTTTTACCTTTTGGGGCGGGTAGAAGAGGTTGTCCGGCTATTACATTCAGCACAGCCAGCATTGAGCTTGCTCTAGCACAACTTCTGCATAGCTTTGATTGGGAGCTTCCTGCTGGTACTGCAGCTGATGATTTGGACATGACAGAAGTTTTTGGCATAACAATGCACAGGATAGCCAATCTGATTGTTGTCGCCAGACCATGGTTTCCCTGA

Protein Analysis

504

Amino Acids

57.15

Weight (kDa)

5.92

Isoelectric Point (pI)

44.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 39 - 489 5.8e-105 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1117
Acc36I ACCTGC 1 cut(s) 1117
AccB1I GGYRCC 1 cut(s) 512
AccB7I CCANNNNNTGG 1 cut(s) 1503
AccII CGCG 1 cut(s) 567
AciI CCGC 2 cut(s) 565, 1319
AclWI GGATC 2 cut(s) 12, 232
AcsI RAATTY 1 cut(s) 1185
AcuI CTGAAG 1 cut(s) 1023
AfaI GTAC 4 cut(s) 282, 1119, 1125, 1420
AfiI CCNNNNNNNGG 8 cut(s) 82, 143, 193, 247, 688, 948, 949, 1503
AflIII ACRYGT 1 cut(s) 906
AgsI TTSAA 7 cut(s) 14, 82, 93, 731, 1072, 1250, 1285
AhlI ACTAGT 1 cut(s) 1120
AjnI CCWGG 1 cut(s) 1199
Alw26I GTCTC 3 cut(s) 385, 848, 1209
AlwI GGATC 2 cut(s) 12, 232
ApeKI GCWGC 3 cut(s) 914, 1099, 1424
ApoI RAATTY 1 cut(s) 1185
ArsI GACNNNNNNTTYG 2 cut(s) 193, 225
AspS9I GGNCC 3 cut(s) 189, 209, 817
AsuC2I CCSGG 1 cut(s) 510
AsuHPI GGTGA 5 cut(s) 146, 167, 245, 931, 1058
AvaII GGWCC 3 cut(s) 189, 209, 817
BanI GGYRCC 1 cut(s) 512
BauI CACGAG 1 cut(s) 821
BbsI GAAGAC 1 cut(s) 358
BbvI GCAGC 3 cut(s) 901, 1086, 1436
BccI CCATC 1 cut(s) 1151
BceAI ACGGC 1 cut(s) 297
BciT130I CCWGG 1 cut(s) 1201
BciVI GTATCC 1 cut(s) 1154
BclI TGATCA 1 cut(s) 607
BcnI CCSGG 1 cut(s) 510
BcoDI GTCTC 3 cut(s) 385, 848, 1209
BcuI ACTAGT 1 cut(s) 1120
BfaI CTAG 7 cut(s) 161, 264, 653, 1121, 1128, 1172, 1376
BfmI CTRYAG 1 cut(s) 1422
BfuAI ACCTGC 1 cut(s) 1117
BfuI GTATCC 1 cut(s) 1154
BisI GCNGC 4 cut(s) 565, 915, 1100, 1425
BlsI GCNGC 4 cut(s) 566, 916, 1101, 1426
BmcAI AGTACT 2 cut(s) 282, 1119
Bme1390I CCNGG 2 cut(s) 510, 1201
Bme18I GGWCC 3 cut(s) 189, 209, 817
BmgT120I GGNCC 3 cut(s) 189, 209, 817
BmiI GGNNCC 1 cut(s) 514
BmrFI CCNGG 2 cut(s) 510, 1201
BmsI GCATC 2 cut(s) 618, 1111
BpiI GAAGAC 1 cut(s) 358
BpmI CTGGAG 1 cut(s) 857
BpuEI CTTGAG 2 cut(s) 437, 653
BpuMI CCSGG 1 cut(s) 510
BsaAI YACGTR 1 cut(s) 401
BsaBI GATNNNNATC 1 cut(s) 1053
BsaI GGTCTC 1 cut(s) 1209
BsaJI CCNNGG 7 cut(s) 192, 273, 687, 876, 1137, 1200, 1502
BsaWI WCCGGW 1 cut(s) 186
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 8 cut(s) 82, 143, 193, 247, 688, 948, 949, 1503
Bse1I ACTGG 2 cut(s) 840, 1115
Bse8I GATNNNNATC 1 cut(s) 1053
BseBI CCWGG 1 cut(s) 1201
BseDI CCNNGG 7 cut(s) 192, 273, 687, 876, 1137, 1200, 1502
BseGI GGATG 3 cut(s) 832, 960, 1102
BseJI GATNNNNATC 1 cut(s) 1053
BseLI CCNNNNNNNGG 8 cut(s) 82, 143, 193, 247, 688, 948, 949, 1503
BseMII CTCAG 2 cut(s) 538, 603
BseNI ACTGG 2 cut(s) 840, 1115
BseRI GAGGAG 1 cut(s) 821
BseXI GCAGC 3 cut(s) 901, 1086, 1436
BsgI GTGCAG 2 cut(s) 461, 1095
Bsh1236I CGCG 1 cut(s) 567
BshNI GGYRCC 1 cut(s) 512
BsiSI CCGG 3 cut(s) 187, 510, 1337
BslI CCNNNNNNNGG 8 cut(s) 82, 143, 193, 247, 688, 948, 949, 1503
BsmAI GTCTC 3 cut(s) 385, 848, 1209
BsmI GAATGC 1 cut(s) 44
Bso31I GGTCTC 1 cut(s) 1209
Bsp143I GATC 5 cut(s) 4, 237, 295, 607, 1048
Bsp19I CCATGG 4 cut(s) 687, 876, 1137, 1502
BspACI CCGC 2 cut(s) 565, 1319
BspCNI CTCAG 2 cut(s) 537, 602
BspFNI CGCG 1 cut(s) 567
BspLI GGNNCC 1 cut(s) 514
BspMAI CTGCAG 1 cut(s) 1426
BspMI ACCTGC 1 cut(s) 1117
BspPI GGATC 2 cut(s) 12, 232
BspT107I GGYRCC 1 cut(s) 512
BspTNI GGTCTC 1 cut(s) 1209
BsrI ACTGG 2 cut(s) 840, 1115
BssECI CCNNGG 7 cut(s) 192, 273, 687, 876, 1137, 1200, 1502
BssMI GATC 5 cut(s) 4, 237, 295, 607, 1048
BssSI CACGAG 1 cut(s) 821
BssT1I CCWWGG 6 cut(s) 192, 273, 687, 876, 1137, 1502
Bst2BI CACGAG 1 cut(s) 821
Bst2UI CCWGG 1 cut(s) 1201
Bst4CI ACNGT 1 cut(s) 247
Bst6I CTCTTC 5 cut(s) 347, 574, 764, 846, 1321
BstBAI YACGTR 1 cut(s) 401
BstC8I GCNNGC 2 cut(s) 1360, 1371
BstDEI CTNAG 2 cut(s) 524, 589
BstDSI CCRYGG 4 cut(s) 687, 876, 1137, 1502
BstENI CCTNNNNNAGG 1 cut(s) 80
BstF5I GGATG 3 cut(s) 832, 960, 1102
BstFNI CGCG 1 cut(s) 567
BstKTI GATC 5 cut(s) 7, 240, 298, 610, 1051
BstMAI GTCTC 3 cut(s) 385, 848, 1209
BstMBI GATC 5 cut(s) 4, 237, 295, 607, 1048
BstMWI GCNNNNNNNGC 4 cut(s) 269, 482, 1359, 1421
BstNI CCWGG 1 cut(s) 1201
BstNSI RCATGY 1 cut(s) 910
BstSCI CCNGG 2 cut(s) 508, 1199
BstSFI CTRYAG 1 cut(s) 1422
BstSNI TACGTA 1 cut(s) 401
BstUI CGCG 1 cut(s) 567
BstV1I GCAGC 3 cut(s) 901, 1086, 1436
BstV2I GAAGAC 1 cut(s) 358
BstX2I RGATCY 2 cut(s) 4, 237
BstXI CCANNNNNNTGG 1 cut(s) 1227
BstYI RGATCY 2 cut(s) 4, 237
BsuI GTATCC 1 cut(s) 1154
BtgI CCRYGG 4 cut(s) 687, 876, 1137, 1502
BtsCI GGATG 3 cut(s) 832, 960, 1102
BtsIMutI CAGTG 1 cut(s) 354
BveI ACCTGC 1 cut(s) 1117
Cac8I GCNNGC 2 cut(s) 1360, 1371
Cfr13I GGNCC 3 cut(s) 189, 209, 817
Csp6I GTAC 4 cut(s) 281, 1118, 1124, 1419
CspCI CAANNNNNGTGG 2 cut(s) 231, 266
CviAII CATG 9 cut(s) 293, 455, 614, 688, 877, 907, 1138, 1441, 1503
CviQI GTAC 4 cut(s) 281, 1118, 1124, 1419
DdeI CTNAG 2 cut(s) 524, 589
DpnI GATC 5 cut(s) 6, 239, 297, 609, 1050
DpnII GATC 5 cut(s) 4, 237, 295, 607, 1048
Eam1104I CTCTTC 5 cut(s) 347, 574, 764, 846, 1321
EarI CTCTTC 5 cut(s) 347, 574, 764, 846, 1321
Eco105I TACGTA 1 cut(s) 401
Eco130I CCWWGG 6 cut(s) 192, 273, 687, 876, 1137, 1502
Eco31I GGTCTC 1 cut(s) 1209
Eco47I GGWCC 3 cut(s) 189, 209, 817
Eco57I CTGAAG 1 cut(s) 1023
EcoNI CCTNNNNNAGG 1 cut(s) 80
EcoO109I RGGNCCY 1 cut(s) 817
EcoRII CCWGG 1 cut(s) 1199
EcoT14I CCWWGG 6 cut(s) 192, 273, 687, 876, 1137, 1502
ErhI CCWWGG 6 cut(s) 192, 273, 687, 876, 1137, 1502
FaeI CATG 9 cut(s) 296, 458, 617, 691, 880, 910, 1141, 1444, 1506
FatI CATG 9 cut(s) 292, 454, 613, 687, 876, 906, 1137, 1440, 1502
FauI CCCGC 1 cut(s) 1312
FbaI TGATCA 1 cut(s) 607
Fnu4HI GCNGC 4 cut(s) 565, 915, 1100, 1425
FokI GGATG 3 cut(s) 839, 967, 1089
Fsp4HI GCNGC 4 cut(s) 565, 915, 1100, 1425
FspBI CTAG 7 cut(s) 161, 264, 653, 1121, 1128, 1172, 1376
GluI GCNGC 4 cut(s) 565, 915, 1100, 1425
GsuI CTGGAG 1 cut(s) 857
HapII CCGG 3 cut(s) 187, 510, 1337
Hin1II CATG 9 cut(s) 296, 458, 617, 691, 880, 910, 1141, 1444, 1506
HinfI GANTC 4 cut(s) 500, 540, 1133, 1223
HpaII CCGG 3 cut(s) 187, 510, 1337
HphI GGTGA 5 cut(s) 146, 167, 245, 931, 1058
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 2 cut(s) 969, 1485
Hpy188III TCNNGA 4 cut(s) 79, 644, 723, 731
Hpy8I GTNNAC 1 cut(s) 317
Hpy99I CGWCG 1 cut(s) 492
HpyAV CCTTC 5 cut(s) 76, 161, 693, 841, 943
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 1 cut(s) 400
HpyF10VI GCNNNNNNNGC 4 cut(s) 269, 482, 1359, 1421
HpyF3I CTNAG 2 cut(s) 524, 589
HpySE526I ACGT 1 cut(s) 400
Hsp92II CATG 9 cut(s) 296, 458, 617, 691, 880, 910, 1141, 1444, 1506
Ksp22I TGATCA 1 cut(s) 607
Kzo9I GATC 5 cut(s) 4, 237, 295, 607, 1048
LmnI GCTCC 2 cut(s) 808, 1405
Lsp1109I GCAGC 3 cut(s) 901, 1086, 1436
LweI GCATC 2 cut(s) 618, 1111
MaeI CTAG 7 cut(s) 161, 264, 653, 1121, 1128, 1172, 1376
MaeII ACGT 1 cut(s) 400
MaeIII GTNAC 1 cut(s) 1147
MalI GATC 5 cut(s) 6, 239, 297, 609, 1050
MboI GATC 5 cut(s) 4, 237, 295, 607, 1048
MflI RGATCY 2 cut(s) 4, 237
MluCI AATT 5 cut(s) 14, 216, 349, 647, 1185
MlyI GAGTC 2 cut(s) 509, 534
MseI TTAA 5 cut(s) 21, 108, 425, 707, 1083
MslI CAYNNNNRTG 1 cut(s) 1463
MspA1I CMGCKG 2 cut(s) 1060, 1427
MspI CCGG 3 cut(s) 187, 510, 1337
MspR9I CCNGG 2 cut(s) 510, 1201
Mva1269I GAATGC 1 cut(s) 44
MvaI CCWGG 1 cut(s) 1201
MvnI CGCG 1 cut(s) 567
MwoI GCNNNNNNNGC 4 cut(s) 269, 482, 1359, 1421
NciI CCSGG 1 cut(s) 510
NcoI CCATGG 4 cut(s) 687, 876, 1137, 1502
NdeII GATC 5 cut(s) 4, 237, 295, 607, 1048
NlaIII CATG 9 cut(s) 296, 458, 617, 691, 880, 910, 1141, 1444, 1506
NlaIV GGNNCC 1 cut(s) 514
NspI RCATGY 1 cut(s) 910
PaqCI CACCTGC 1 cut(s) 1117
PciI ACATGT 1 cut(s) 906
PcsI WCGNNNNNNNCGW 1 cut(s) 484
PctI GAATGC 1 cut(s) 44
PfeI GAWTC 2 cut(s) 1133, 1223
PflMI CCANNNNNTGG 1 cut(s) 1503
PkrI GCNGC 4 cut(s) 566, 916, 1101, 1426
PleI GAGTC 2 cut(s) 508, 534
PpsI GAGTC 2 cut(s) 508, 534
Ppu21I YACGTR 1 cut(s) 401
PpuMI RGGWCCY 1 cut(s) 817
PscI ACATGT 1 cut(s) 906
Psp5II RGGWCCY 1 cut(s) 817
Psp6I CCWGG 1 cut(s) 1199
PspGI CCWGG 1 cut(s) 1199
PspN4I GGNNCC 1 cut(s) 514
PspPI GGNCC 3 cut(s) 189, 209, 817
PspPPI RGGWCCY 1 cut(s) 817
PstI CTGCAG 1 cut(s) 1426
PsuI RGATCY 2 cut(s) 4, 237
PvuII CAGCTG 2 cut(s) 1060, 1427
RsaI GTAC 4 cut(s) 282, 1119, 1125, 1420
RsaNI GTAC 4 cut(s) 281, 1118, 1124, 1419
RseI CAYNNNNRTG 1 cut(s) 1463
SaqAI TTAA 5 cut(s) 21, 108, 425, 707, 1083
SatI GCNGC 4 cut(s) 565, 915, 1100, 1425
Sau3AI GATC 5 cut(s) 4, 237, 295, 607, 1048
Sau96I GGNCC 3 cut(s) 189, 209, 817
ScaI AGTACT 2 cut(s) 282, 1119
SchI GAGTC 2 cut(s) 509, 534
ScrFI CCNGG 2 cut(s) 510, 1201
SfaNI GCATC 2 cut(s) 618, 1111
SfcI CTRYAG 1 cut(s) 1422
SinI GGWCC 3 cut(s) 189, 209, 817
SmiMI CAYNNNNRTG 1 cut(s) 1463
SmlI CTYRAG 2 cut(s) 416, 632
SmoI CTYRAG 2 cut(s) 416, 632
SnaBI TACGTA 1 cut(s) 401
SpeI ACTAGT 1 cut(s) 1120
Sse9I AATT 5 cut(s) 14, 216, 349, 647, 1185
SsiI CCGC 2 cut(s) 565, 1319
SspMI CTAG 7 cut(s) 161, 264, 653, 1121, 1128, 1172, 1376
StyD4I CCNGG 2 cut(s) 508, 1199
StyI CCWWGG 6 cut(s) 192, 273, 687, 876, 1137, 1502
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 1 cut(s) 403
TaqI TCGA 2 cut(s) 745, 762
TasI AATT 5 cut(s) 14, 216, 349, 647, 1185
TatI WGTACW 2 cut(s) 280, 1117
TauI GCSGC 1 cut(s) 567
TfiI GAWTC 2 cut(s) 1133, 1223
Tru1I TTAA 5 cut(s) 21, 108, 425, 707, 1083
Tru9I TTAA 5 cut(s) 21, 108, 425, 707, 1083
TscAI CASTG 1 cut(s) 361
TseI GCWGC 3 cut(s) 914, 1099, 1424
TspDTI ATGAA 5 cut(s) 246, 731, 925, 986, 1043
TspRI CASTG 1 cut(s) 361
Van91I CCANNNNNTGG 1 cut(s) 1503
VpaK11BI GGWCC 3 cut(s) 189, 209, 817
XagI CCTNNNNNAGG 1 cut(s) 80
XapI RAATTY 1 cut(s) 1185
XceI RCATGY 1 cut(s) 910
XspI CTAG 7 cut(s) 161, 264, 653, 1121, 1128, 1172, 1376
ZrmI AGTACT 2 cut(s) 282, 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.