RLG00000004391

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013668
Physical Location & Seq
Forward (+)
58964228 .. 58966344
2117 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004391

Sequence Viewer

Length: 1515 bp
ATGGATCTTCTTCAATTTCTTAACATACCCTCTTTGCTTTTGGCATTCAGTTTCCTCGTAGTGTTGCTTACATTCCTCTTCAAGGATAGTTCAAGAAAAAGAAAACTTAGACTCCCACCAAGCCCTCCAAAGCTACCTGTTGTTGGCAACCTTCACCAGCTAGGAAACAAGCCTCATCTATCTCTCCGGTCCTTGGCACAAAAGTATGGTCCAATAATTTACTTACAACTTGGTGAGATCCCAACTGTGGTGGTTTCATCAGCTAGACTTGCCAAGGAAGTACTGAAAACCCATGATCTTGCACTATCAAGCCGTCCACAAATCTTTTCAGCCAAACACCTCTTCTATAATTGCACTGATGTTGTCTTCTCCCCTTATGGTGCTTATTGGAGACACATACGTAAGATTTGCATACTTGAGCTGTTAAGTGCCAAAAGAGTGCAGTCATTTAGCCATGCCAGAGAAGAAGAAGTTACTCGTCTGGCTTGTAGGGTTGCAGAGTCCTATCCCGGCACCACTAATCTCAGCAAGTTACTTGGACTCTATGCAAATGATATTCTCTGCCGCGTAGCATTTGGAAGAGATTTCTCAGGTGGTGGAGAGTATGATCAGCATGATTTCCAAAAGATGCTTGAAGAATATCAGGAATTGCTTGGTGGATTTTCTATTGGAGATTTCTTCCCTTCCATGGAGTTCCTCCACACCTTAACAGGAATGAAATCAAGACTTCAAGACACATTCGGTCGATTTGATAAACTCTTCGACCAGATTTTGACTGAACATCTCAATCCCAAAAGAGAAACAGAGGAGCATAAGGACCTGGTGGATGTTTTACTGGAGATACAGAAGAAAGAGTCTGCTGATATGCCTCTAACCATGGATAATGTCAAGGCTATTATCTTGGACATGTTTGCTGCTGGAACAGACACAACCTTCATCACCCTTGATTGGGGAATGACTGAACTTTTGATGAACCCCAAAGTCCTCCAAAAAGCACAAGCTGAAGTAAGAGGTGTTGTTGGGGAAAGAAGAGTTGTGTTAGAGGGTGATCTTCATCAGCTGGACTATTTGAAAGCTGTCATTAAAGAGATATTTCGGCTGCATCCACCTGCACCAGTACTAGTACCTCGAGAATCCATGGAAGATGTAACCATTGATGGATACGACATTCTAGCCAGAACAAGAATTTTTGTCAATGCCTGGGCAATAGGGAGAGACCCAGAATCTTGGGAAAACCCAGAAAAGTTTGAACCAGAAAGATTTATGGGTAGCACTATTGATTTCAAGGGGCAGGATTTTGAGCTTCTACCTTTTGGGGCGGGTAGAAGAGGTTGTCCGGCTATTACATTCAGCACAGCCAGCATTGAGCTTGCTCTAGCGCAACTTCTGCATAGCTTTGATTGGGAGCTTCCTGCTGGTACTGCAGCTGATGATTTGGACATGACAGAAGTTTTTGGCATAACAATGCACAGGATAGCCAATCTGATTGTTGTTGCCAGGCCACGGTTTCCCTGA

Protein Analysis

505

Amino Acids

57.1

Weight (kDa)

6.0

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 39 - 489 1.4e-103 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1117
Acc36I ACCTGC 1 cut(s) 1117
AccB1I GGYRCC 1 cut(s) 512
AccII CGCG 1 cut(s) 567
AciI CCGC 2 cut(s) 565, 1319
AclWI GGATC 2 cut(s) 12, 232
AcsI RAATTY 1 cut(s) 1185
AcuI CTGAAG 1 cut(s) 1023
AfaI GTAC 4 cut(s) 282, 1119, 1125, 1420
AfiI CCNNNNNNNGG 8 cut(s) 82, 143, 193, 247, 688, 948, 949, 1503
AflIII ACRYGT 1 cut(s) 906
AgsI TTSAA 8 cut(s) 14, 82, 93, 635, 731, 1072, 1250, 1285
AhlI ACTAGT 1 cut(s) 1120
AjnI CCWGG 3 cut(s) 819, 1199, 1496
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
Alw26I GTCTC 2 cut(s) 385, 1209
AlwI GGATC 2 cut(s) 12, 232
Ama87I CYCGRG 1 cut(s) 1128
AoxI GGCC 1 cut(s) 1499
ApeKI GCWGC 3 cut(s) 914, 1099, 1424
ApoI RAATTY 1 cut(s) 1185
ArsI GACNNNNNNTTYG 4 cut(s) 193, 225, 949, 981
AspLEI GCGC 1 cut(s) 1381
AspS9I GGNCC 3 cut(s) 189, 209, 817
AsuC2I CCSGG 1 cut(s) 510
AsuHPI GGTGA 4 cut(s) 146, 245, 931, 1058
AvaI CYCGRG 1 cut(s) 1128
AvaII GGWCC 3 cut(s) 189, 209, 817
BanI GGYRCC 1 cut(s) 512
BbsI GAAGAC 1 cut(s) 358
BbvI GCAGC 3 cut(s) 901, 1086, 1436
BccI CCATC 1 cut(s) 1151
BceAI ACGGC 1 cut(s) 297
BciT130I CCWGG 3 cut(s) 821, 1201, 1498
BciVI GTATCC 1 cut(s) 1154
BclI TGATCA 1 cut(s) 607
BcnI CCSGG 1 cut(s) 510
BcoDI GTCTC 2 cut(s) 385, 1209
BcuI ACTAGT 1 cut(s) 1120
BfaI CTAG 5 cut(s) 161, 264, 1121, 1172, 1376
BfmI CTRYAG 1 cut(s) 1422
BfuAI ACCTGC 1 cut(s) 1117
BfuI GTATCC 1 cut(s) 1154
BisI GCNGC 4 cut(s) 565, 915, 1100, 1425
BlsI GCNGC 4 cut(s) 566, 916, 1101, 1426
BmcAI AGTACT 2 cut(s) 282, 1119
Bme1390I CCNGG 4 cut(s) 510, 821, 1201, 1498
Bme18I GGWCC 3 cut(s) 189, 209, 817
BmeT110I CYCGRG 1 cut(s) 1128
BmgT120I GGNCC 3 cut(s) 189, 209, 817
BmiI GGNNCC 1 cut(s) 514
BmrFI CCNGG 4 cut(s) 510, 821, 1201, 1498
BmsI GCATC 2 cut(s) 618, 1111
BpiI GAAGAC 1 cut(s) 358
BpmI CTGGAG 1 cut(s) 857
BpuEI CTTGAG 1 cut(s) 437
BpuMI CCSGG 1 cut(s) 510
BsaAI YACGTR 1 cut(s) 401
BsaBI GATNNNNATC 1 cut(s) 1053
BsaI GGTCTC 1 cut(s) 1209
BsaJI CCNNGG 7 cut(s) 192, 273, 687, 876, 1137, 1200, 1502
BsaWI WCCGGW 1 cut(s) 186
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 8 cut(s) 82, 143, 193, 247, 688, 948, 949, 1503
Bse1I ACTGG 2 cut(s) 840, 1115
Bse8I GATNNNNATC 1 cut(s) 1053
BseBI CCWGG 3 cut(s) 821, 1201, 1498
BseDI CCNNGG 7 cut(s) 192, 273, 687, 876, 1137, 1200, 1502
BseGI GGATG 2 cut(s) 832, 1102
BseJI GATNNNNATC 1 cut(s) 1053
BseLI CCNNNNNNNGG 8 cut(s) 82, 143, 193, 247, 688, 948, 949, 1503
BseMII CTCAG 2 cut(s) 538, 603
BseNI ACTGG 2 cut(s) 840, 1115
BseRI GAGGAG 1 cut(s) 821
BseXI GCAGC 3 cut(s) 901, 1086, 1436
BsgI GTGCAG 2 cut(s) 461, 1095
Bsh1236I CGCG 1 cut(s) 567
Bsh1285I CGRYCG 1 cut(s) 745
BshFI GGCC 1 cut(s) 1501
BshNI GGYRCC 1 cut(s) 512
BsiEI CGRYCG 1 cut(s) 745
BsiHKCI CYCGRG 1 cut(s) 1128
BsiSI CCGG 3 cut(s) 187, 510, 1337
BslI CCNNNNNNNGG 8 cut(s) 82, 143, 193, 247, 688, 948, 949, 1503
BsmAI GTCTC 2 cut(s) 385, 1209
BsmI GAATGC 1 cut(s) 44
BsnI GGCC 1 cut(s) 1501
Bso31I GGTCTC 1 cut(s) 1209
BsoBI CYCGRG 1 cut(s) 1128
Bsp143I GATC 5 cut(s) 4, 237, 295, 607, 1048
Bsp19I CCATGG 3 cut(s) 687, 876, 1137
BspACI CCGC 2 cut(s) 565, 1319
BspANI GGCC 1 cut(s) 1501
BspCNI CTCAG 2 cut(s) 537, 602
BspFNI CGCG 1 cut(s) 567
BspLI GGNNCC 1 cut(s) 514
BspMAI CTGCAG 1 cut(s) 1426
BspMI ACCTGC 1 cut(s) 1117
BspPI GGATC 2 cut(s) 12, 232
BspT107I GGYRCC 1 cut(s) 512
BspTNI GGTCTC 1 cut(s) 1209
BsrI ACTGG 2 cut(s) 840, 1115
BssECI CCNNGG 7 cut(s) 192, 273, 687, 876, 1137, 1200, 1502
BssMI GATC 5 cut(s) 4, 237, 295, 607, 1048
BssT1I CCWWGG 5 cut(s) 192, 273, 687, 876, 1137
Bst2UI CCWGG 3 cut(s) 821, 1201, 1498
Bst4CI ACNGT 2 cut(s) 247, 1506
Bst6I CTCTTC 6 cut(s) 83, 347, 574, 764, 1024, 1321
BstAPI GCANNNNNTGC 1 cut(s) 1387
BstBAI YACGTR 1 cut(s) 401
BstC8I GCNNGC 2 cut(s) 1360, 1371
BstDEI CTNAG 3 cut(s) 107, 524, 589
BstDSI CCRYGG 4 cut(s) 687, 876, 1137, 1502
BstENI CCTNNNNNAGG 1 cut(s) 80
BstF5I GGATG 2 cut(s) 832, 1102
BstFNI CGCG 1 cut(s) 567
BstHHI GCGC 1 cut(s) 1381
BstKTI GATC 5 cut(s) 7, 240, 298, 610, 1051
BstMAI GTCTC 2 cut(s) 385, 1209
BstMBI GATC 5 cut(s) 4, 237, 295, 607, 1048
BstMCI CGRYCG 1 cut(s) 745
BstMWI GCNNNNNNNGC 4 cut(s) 269, 1359, 1387, 1421
BstNI CCWGG 3 cut(s) 821, 1201, 1498
BstNSI RCATGY 1 cut(s) 910
BstSCI CCNGG 4 cut(s) 508, 819, 1199, 1496
BstSFI CTRYAG 1 cut(s) 1422
BstSNI TACGTA 1 cut(s) 401
BstUI CGCG 1 cut(s) 567
BstV1I GCAGC 3 cut(s) 901, 1086, 1436
BstV2I GAAGAC 1 cut(s) 358
BstX2I RGATCY 2 cut(s) 4, 237
BstXI CCANNNNNNTGG 1 cut(s) 1227
BstYI RGATCY 2 cut(s) 4, 237
BsuI GTATCC 1 cut(s) 1154
BsuRI GGCC 1 cut(s) 1501
BtgI CCRYGG 4 cut(s) 687, 876, 1137, 1502
BtsCI GGATG 2 cut(s) 832, 1102
BtsIMutI CAGTG 1 cut(s) 354
BveI ACCTGC 1 cut(s) 1117
Cac8I GCNNGC 2 cut(s) 1360, 1371
CfoI GCGC 1 cut(s) 1381
Cfr13I GGNCC 3 cut(s) 189, 209, 817
CsiI ACCWGGT 1 cut(s) 819
Csp6I GTAC 4 cut(s) 281, 1118, 1124, 1419
CspCI CAANNNNNGTGG 2 cut(s) 231, 266
CviAII CATG 8 cut(s) 293, 455, 614, 688, 877, 907, 1138, 1441
CviQI GTAC 4 cut(s) 281, 1118, 1124, 1419
DdeI CTNAG 3 cut(s) 107, 524, 589
DpnI GATC 5 cut(s) 6, 239, 297, 609, 1050
DpnII GATC 5 cut(s) 4, 237, 295, 607, 1048
Eam1104I CTCTTC 6 cut(s) 83, 347, 574, 764, 1024, 1321
EarI CTCTTC 6 cut(s) 83, 347, 574, 764, 1024, 1321
Eco105I TACGTA 1 cut(s) 401
Eco130I CCWWGG 5 cut(s) 192, 273, 687, 876, 1137
Eco31I GGTCTC 1 cut(s) 1209
Eco47I GGWCC 3 cut(s) 189, 209, 817
Eco57I CTGAAG 1 cut(s) 1023
Eco88I CYCGRG 1 cut(s) 1128
EcoNI CCTNNNNNAGG 1 cut(s) 80
EcoO109I RGGNCCY 1 cut(s) 817
EcoRII CCWGG 3 cut(s) 819, 1199, 1496
EcoT14I CCWWGG 5 cut(s) 192, 273, 687, 876, 1137
ErhI CCWWGG 5 cut(s) 192, 273, 687, 876, 1137
FaeI CATG 8 cut(s) 296, 458, 617, 691, 880, 910, 1141, 1444
FatI CATG 8 cut(s) 292, 454, 613, 687, 876, 906, 1137, 1440
FauI CCCGC 1 cut(s) 1312
FbaI TGATCA 1 cut(s) 607
Fnu4HI GCNGC 4 cut(s) 565, 915, 1100, 1425
FokI GGATG 2 cut(s) 839, 1089
Fsp4HI GCNGC 4 cut(s) 565, 915, 1100, 1425
FspBI CTAG 5 cut(s) 161, 264, 1121, 1172, 1376
GlaI GCGC 1 cut(s) 1380
GluI GCNGC 4 cut(s) 565, 915, 1100, 1425
GsuI CTGGAG 1 cut(s) 857
HaeIII GGCC 1 cut(s) 1501
HapII CCGG 3 cut(s) 187, 510, 1337
HhaI GCGC 1 cut(s) 1381
Hin1II CATG 8 cut(s) 296, 458, 617, 691, 880, 910, 1141, 1444
Hin6I GCGC 1 cut(s) 1379
HinP1I GCGC 1 cut(s) 1379
HinfI GANTC 6 cut(s) 111, 500, 540, 854, 1133, 1223
HpaII CCGG 3 cut(s) 187, 510, 1337
HphI GGTGA 4 cut(s) 146, 245, 931, 1058
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 1 cut(s) 1485
Hpy188III TCNNGA 5 cut(s) 93, 644, 723, 731, 1130
Hpy8I GTNNAC 1 cut(s) 317
HpyAV CCTTC 3 cut(s) 161, 693, 943
HpyCH4III ACNGT 2 cut(s) 247, 1506
HpyCH4IV ACGT 1 cut(s) 400
HpyF10VI GCNNNNNNNGC 4 cut(s) 269, 1359, 1387, 1421
HpyF3I CTNAG 3 cut(s) 107, 524, 589
HpySE526I ACGT 1 cut(s) 400
Hsp92II CATG 8 cut(s) 296, 458, 617, 691, 880, 910, 1141, 1444
HspAI GCGC 1 cut(s) 1379
Ksp22I TGATCA 1 cut(s) 607
Kzo9I GATC 5 cut(s) 4, 237, 295, 607, 1048
LmnI GCTCC 2 cut(s) 808, 1405
Lsp1109I GCAGC 3 cut(s) 901, 1086, 1436
LweI GCATC 2 cut(s) 618, 1111
MabI ACCWGGT 1 cut(s) 819
MaeI CTAG 5 cut(s) 161, 264, 1121, 1172, 1376
MaeII ACGT 1 cut(s) 400
MaeIII GTNAC 3 cut(s) 472, 531, 1147
MalI GATC 5 cut(s) 6, 239, 297, 609, 1050
MboI GATC 5 cut(s) 4, 237, 295, 607, 1048
MflI RGATCY 2 cut(s) 4, 237
MluCI AATT 5 cut(s) 14, 216, 349, 647, 1185
MlyI GAGTC 4 cut(s) 105, 509, 534, 863
MseI TTAA 4 cut(s) 21, 425, 707, 1083
MslI CAYNNNNRTG 1 cut(s) 1463
MspA1I CMGCKG 2 cut(s) 1060, 1427
MspI CCGG 3 cut(s) 187, 510, 1337
MspR9I CCNGG 4 cut(s) 510, 821, 1201, 1498
Mva1269I GAATGC 1 cut(s) 44
MvaI CCWGG 3 cut(s) 821, 1201, 1498
MvnI CGCG 1 cut(s) 567
MwoI GCNNNNNNNGC 4 cut(s) 269, 1359, 1387, 1421
NciI CCSGG 1 cut(s) 510
NcoI CCATGG 3 cut(s) 687, 876, 1137
NdeII GATC 5 cut(s) 4, 237, 295, 607, 1048
NlaIII CATG 8 cut(s) 296, 458, 617, 691, 880, 910, 1141, 1444
NlaIV GGNNCC 1 cut(s) 514
NspI RCATGY 1 cut(s) 910
PaeR7I CTCGAG 1 cut(s) 1128
PaqCI CACCTGC 1 cut(s) 1117
PciI ACATGT 1 cut(s) 906
PctI GAATGC 1 cut(s) 44
PfeI GAWTC 2 cut(s) 1133, 1223
PkrI GCNGC 4 cut(s) 566, 916, 1101, 1426
PleI GAGTC 4 cut(s) 105, 508, 534, 862
PpsI GAGTC 4 cut(s) 105, 508, 534, 862
Ppu21I YACGTR 1 cut(s) 401
PpuMI RGGWCCY 1 cut(s) 817
PscI ACATGT 1 cut(s) 906
Psp5II RGGWCCY 1 cut(s) 817
Psp6I CCWGG 3 cut(s) 819, 1199, 1496
PspGI CCWGG 3 cut(s) 819, 1199, 1496
PspN4I GGNNCC 1 cut(s) 514
PspPI GGNCC 3 cut(s) 189, 209, 817
PspPPI RGGWCCY 1 cut(s) 817
PstI CTGCAG 1 cut(s) 1426
PsuI RGATCY 2 cut(s) 4, 237
PvuII CAGCTG 2 cut(s) 1060, 1427
RsaI GTAC 4 cut(s) 282, 1119, 1125, 1420
RsaNI GTAC 4 cut(s) 281, 1118, 1124, 1419
RseI CAYNNNNRTG 1 cut(s) 1463
SaqAI TTAA 4 cut(s) 21, 425, 707, 1083
SatI GCNGC 4 cut(s) 565, 915, 1100, 1425
Sau3AI GATC 5 cut(s) 4, 237, 295, 607, 1048
Sau96I GGNCC 3 cut(s) 189, 209, 817
ScaI AGTACT 2 cut(s) 282, 1119
SchI GAGTC 4 cut(s) 105, 509, 534, 863
ScrFI CCNGG 4 cut(s) 510, 821, 1201, 1498
SexAI ACCWGGT 1 cut(s) 819
SfaNI GCATC 2 cut(s) 618, 1111
SfcI CTRYAG 1 cut(s) 1422
Sfr274I CTCGAG 1 cut(s) 1128
SinI GGWCC 3 cut(s) 189, 209, 817
SlaI CTCGAG 1 cut(s) 1128
SmiMI CAYNNNNRTG 1 cut(s) 1463
SmlI CTYRAG 2 cut(s) 416, 1128
SmoI CTYRAG 2 cut(s) 416, 1128
SnaBI TACGTA 1 cut(s) 401
SpeI ACTAGT 1 cut(s) 1120
Sse9I AATT 5 cut(s) 14, 216, 349, 647, 1185
SsiI CCGC 2 cut(s) 565, 1319
SspMI CTAG 5 cut(s) 161, 264, 1121, 1172, 1376
StyD4I CCNGG 4 cut(s) 508, 819, 1199, 1496
StyI CCWWGG 5 cut(s) 192, 273, 687, 876, 1137
TaaI ACNGT 2 cut(s) 247, 1506
TaiI ACGT 1 cut(s) 403
TaqI TCGA 3 cut(s) 745, 762, 1129
TaqII GACCGA 1 cut(s) 731
TasI AATT 5 cut(s) 14, 216, 349, 647, 1185
TatI WGTACW 2 cut(s) 280, 1117
TauI GCSGC 1 cut(s) 567
TfiI GAWTC 2 cut(s) 1133, 1223
Tru1I TTAA 4 cut(s) 21, 425, 707, 1083
Tru9I TTAA 4 cut(s) 21, 425, 707, 1083
TscAI CASTG 1 cut(s) 361
TseI GCWGC 3 cut(s) 914, 1099, 1424
TspDTI ATGAA 5 cut(s) 246, 731, 925, 986, 1043
TspRI CASTG 1 cut(s) 361
VpaK11BI GGWCC 3 cut(s) 189, 209, 817
XagI CCTNNNNNAGG 1 cut(s) 80
XapI RAATTY 1 cut(s) 1185
XceI RCATGY 1 cut(s) 910
XhoI CTCGAG 1 cut(s) 1128
XspI CTAG 5 cut(s) 161, 264, 1121, 1172, 1376
ZrmI AGTACT 2 cut(s) 282, 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.