Rh7BG125500

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0013668
Physical Location & Seq
Reverse (-)
9309340 .. 9310329
990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG125500.1

Sequence Viewer

Length: 990 bp
ATGGATGTTCTTCAATTTCTTAACATACCCTCTTTGCTTTTGGCATTCAGTTTCCTCATAGTGTTGCTTACTTTCCTCTTCAAGGATAGTTCAAGAAAAAGAAAACTTAAACTCCCACCAAGCCCTCCAAAGCTACCTGTTGTTGGCAACCTTCACCAGCTAGGAAACCAGCCTCACCTATCTCTCCGGTCCTTGGCACAAAAGTATGGTCCAATAATTTACTTACAACTTGGTGAGATCCCAACTGTGGTGGTTTCATCAGCTAGACTTGCCAAGGAAGTACTGAAAACCCATGATCTTGCACTATCAAGCCGTCCACAAATCTTTTCAGCCAAACACCTCTTCTATAATTGCACTGATGTTGTCTTCTCCCCTTATGGTGCTTATTGGAGACACATACGTAAGATTTGCATACTTGAGCTGTTAAGTGCCAAAAGAGTGCAGTCATTTAGCCATGCCAGAGAAGAAGAAGTTGCTCGTCTGGCTCGTCGGGTTGCAGAGTCCTATCCTGGCACCACTAATCTCAGCAAGTTGCTTGGACTCTATGCAAATGATATTCTCTGCCGCGTAGCATTTGGAAGAGATTTCTCAGGTGGTGGAGAGTATGATCAGCATGATTTCCAAAAGATGCTTGAGGAATATCAGGAATTGCTTGGTGGATTTTCTATTGGAGATTTCTTCCCTTCCATGGAGTTCCTCCACACCTTAACAGGAATGAAATCAAGACTTCAAGACACATTCAGTCGATTTGATAAACTCTTCGACCAGATTTTGACTGAACATCTCAATCCCAAAAGAGAAAAAGAGGAGCATAAGGACCTCATGGATGTTTTACTGGAGATACAGAAGGAAGAGACTGCTGATATGCCTCTAACCATGGATAATGTCAAGGCTATTATTTTGGTCAGTGTAGACTCTTTGATGCATAAACTATATTCATTCACAGTAATGAAATCCATATTAAATTATTTTCATATGAATTGCACTTAA

Protein Analysis

329

Amino Acids

37.72

Weight (kDa)

8.52

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 39 - 301 9.1e-38 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 512
AccI GTMKAC 1 cut(s) 912
AccII CGCG 1 cut(s) 567
AciI CCGC 1 cut(s) 565
AclWI GGATC 1 cut(s) 232
AfaI GTAC 1 cut(s) 282
AfiI CCNNNNNNNGG 5 cut(s) 82, 143, 193, 247, 688
AgsI TTSAA 4 cut(s) 14, 82, 93, 731
AjnI CCWGG 1 cut(s) 508
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AluBI AGCT 4 cut(s) 133, 160, 263, 421
AluI AGCT 4 cut(s) 133, 160, 263, 421
Alw26I GTCTC 2 cut(s) 385, 848
AlwI GGATC 1 cut(s) 232
ArsI GACNNNNNNTTYG 2 cut(s) 193, 225
AspS9I GGNCC 3 cut(s) 189, 209, 817
AsuHPI GGTGA 3 cut(s) 146, 167, 245
AvaII GGWCC 3 cut(s) 189, 209, 817
BanI GGYRCC 1 cut(s) 512
BbsI GAAGAC 1 cut(s) 358
BceAI ACGGC 1 cut(s) 297
BciT130I CCWGG 1 cut(s) 510
BclI TGATCA 1 cut(s) 607
BcoDI GTCTC 2 cut(s) 385, 848
BfaI CTAG 2 cut(s) 161, 264
BisI GCNGC 1 cut(s) 565
BlsI GCNGC 1 cut(s) 566
BmcAI AGTACT 1 cut(s) 282
Bme1390I CCNGG 1 cut(s) 510
Bme18I GGWCC 3 cut(s) 189, 209, 817
BmgT120I GGNCC 3 cut(s) 189, 209, 817
BmiI GGNNCC 1 cut(s) 514
BmrFI CCNGG 1 cut(s) 510
BmsI GCATC 2 cut(s) 618, 912
BpiI GAAGAC 1 cut(s) 358
BpmI CTGGAG 1 cut(s) 857
BpuEI CTTGAG 2 cut(s) 437, 653
BsaAI YACGTR 1 cut(s) 401
BsaJI CCNNGG 4 cut(s) 192, 273, 687, 876
BsaWI WCCGGW 1 cut(s) 186
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 143, 193, 247, 688
Bse1I ACTGG 1 cut(s) 840
BseBI CCWGG 1 cut(s) 510
BseDI CCNNGG 4 cut(s) 192, 273, 687, 876
BseGI GGATG 2 cut(s) 10, 832
BseLI CCNNNNNNNGG 5 cut(s) 82, 143, 193, 247, 688
BseMII CTCAG 2 cut(s) 538, 603
BseNI ACTGG 1 cut(s) 840
BseRI GAGGAG 1 cut(s) 821
BsgI GTGCAG 1 cut(s) 461
Bsh1236I CGCG 1 cut(s) 567
BshNI GGYRCC 1 cut(s) 512
BsiSI CCGG 1 cut(s) 187
BslI CCNNNNNNNGG 5 cut(s) 82, 143, 193, 247, 688
BsmAI GTCTC 2 cut(s) 385, 848
BsmI GAATGC 1 cut(s) 44
Bsp143I GATC 3 cut(s) 237, 295, 607
Bsp19I CCATGG 2 cut(s) 687, 876
BspACI CCGC 1 cut(s) 565
BspCNI CTCAG 2 cut(s) 537, 602
BspFNI CGCG 1 cut(s) 567
BspLI GGNNCC 1 cut(s) 514
BspPI GGATC 1 cut(s) 232
BspT107I GGYRCC 1 cut(s) 512
BsrI ACTGG 1 cut(s) 840
BssECI CCNNGG 4 cut(s) 192, 273, 687, 876
BssMI GATC 3 cut(s) 237, 295, 607
BssT1I CCWWGG 4 cut(s) 192, 273, 687, 876
Bst2UI CCWGG 1 cut(s) 510
Bst4CI ACNGT 2 cut(s) 247, 946
Bst6I CTCTTC 5 cut(s) 83, 347, 574, 764, 846
BstBAI YACGTR 1 cut(s) 401
BstDEI CTNAG 2 cut(s) 524, 589
BstDSI CCRYGG 2 cut(s) 687, 876
BstENI CCTNNNNNAGG 1 cut(s) 80
BstF5I GGATG 2 cut(s) 10, 832
BstFNI CGCG 1 cut(s) 567
BstKTI GATC 3 cut(s) 240, 298, 610
BstMAI GTCTC 2 cut(s) 385, 848
BstMBI GATC 3 cut(s) 237, 295, 607
BstMWI GCNNNNNNNGC 2 cut(s) 269, 482
BstNI CCWGG 1 cut(s) 510
BstSCI CCNGG 1 cut(s) 508
BstSNI TACGTA 1 cut(s) 401
BstUI CGCG 1 cut(s) 567
BstV2I GAAGAC 1 cut(s) 358
BstX2I RGATCY 1 cut(s) 237
BstYI RGATCY 1 cut(s) 237
BtgI CCRYGG 2 cut(s) 687, 876
BtsCI GGATG 2 cut(s) 10, 832
BtsIMutI CAGTG 2 cut(s) 354, 913
Cfr13I GGNCC 3 cut(s) 189, 209, 817
Csp6I GTAC 1 cut(s) 281
CspCI CAANNNNNGTGG 2 cut(s) 231, 266
CviAII CATG 6 cut(s) 293, 455, 614, 688, 823, 877
CviQI GTAC 1 cut(s) 281
DdeI CTNAG 2 cut(s) 524, 589
DpnI GATC 3 cut(s) 239, 297, 609
DpnII GATC 3 cut(s) 237, 295, 607
Eam1104I CTCTTC 5 cut(s) 83, 347, 574, 764, 846
EarI CTCTTC 5 cut(s) 83, 347, 574, 764, 846
Eco105I TACGTA 1 cut(s) 401
Eco130I CCWWGG 4 cut(s) 192, 273, 687, 876
Eco47I GGWCC 3 cut(s) 189, 209, 817
EcoNI CCTNNNNNAGG 1 cut(s) 80
EcoO109I RGGNCCY 1 cut(s) 817
EcoRII CCWGG 1 cut(s) 508
EcoT14I CCWWGG 4 cut(s) 192, 273, 687, 876
EcoT22I ATGCAT 1 cut(s) 927
ErhI CCWWGG 4 cut(s) 192, 273, 687, 876
FaeI CATG 6 cut(s) 296, 458, 617, 691, 826, 880
FatI CATG 6 cut(s) 292, 454, 613, 687, 822, 876
FauNDI CATATG 1 cut(s) 975
FbaI TGATCA 1 cut(s) 607
FblI GTMKAC 1 cut(s) 912
Fnu4HI GCNGC 1 cut(s) 565
FokI GGATG 2 cut(s) 17, 839
Fsp4HI GCNGC 1 cut(s) 565
FspBI CTAG 2 cut(s) 161, 264
GluI GCNGC 1 cut(s) 565
GsuI CTGGAG 1 cut(s) 857
HapII CCGG 1 cut(s) 187
Hin1II CATG 6 cut(s) 296, 458, 617, 691, 826, 880
HinfI GANTC 3 cut(s) 500, 540, 914
HpaII CCGG 1 cut(s) 187
HphI GGTGA 3 cut(s) 146, 167, 245
Hpy166II GTNNAC 2 cut(s) 317, 913
Hpy188III TCNNGA 4 cut(s) 93, 644, 723, 731
Hpy8I GTNNAC 2 cut(s) 317, 913
Hpy99I CGWCG 1 cut(s) 492
HpyAV CCTTC 3 cut(s) 161, 693, 841
HpyCH4III ACNGT 2 cut(s) 247, 946
HpyCH4IV ACGT 1 cut(s) 400
HpyCH4V TGCA 8 cut(s) 302, 354, 411, 442, 497, 548, 925, 984
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 482
HpyF3I CTNAG 2 cut(s) 524, 589
HpySE526I ACGT 1 cut(s) 400
Hsp92II CATG 6 cut(s) 296, 458, 617, 691, 826, 880
Ksp22I TGATCA 1 cut(s) 607
Kzo9I GATC 3 cut(s) 237, 295, 607
LmnI GCTCC 1 cut(s) 808
LweI GCATC 2 cut(s) 618, 912
MaeI CTAG 2 cut(s) 161, 264
MaeII ACGT 1 cut(s) 400
MalI GATC 3 cut(s) 239, 297, 609
MboI GATC 3 cut(s) 237, 295, 607
MboII GAAGA 9 cut(s) 70, 334, 358, 476, 479, 591, 670, 751, 863
MflI RGATCY 1 cut(s) 237
MluCI AATT 6 cut(s) 14, 216, 349, 647, 964, 979
MlyI GAGTC 3 cut(s) 509, 534, 908
Mph1103I ATGCAT 1 cut(s) 927
MseI TTAA 6 cut(s) 21, 108, 425, 707, 962, 988
MslI CAYNNNNRTG 1 cut(s) 947
MspI CCGG 1 cut(s) 187
MspR9I CCNGG 1 cut(s) 510
Mva1269I GAATGC 1 cut(s) 44
MvaI CCWGG 1 cut(s) 510
MvnI CGCG 1 cut(s) 567
MwoI GCNNNNNNNGC 2 cut(s) 269, 482
NcoI CCATGG 2 cut(s) 687, 876
NdeI CATATG 1 cut(s) 975
NdeII GATC 3 cut(s) 237, 295, 607
NlaIII CATG 6 cut(s) 296, 458, 617, 691, 826, 880
NlaIV GGNNCC 1 cut(s) 514
NsiI ATGCAT 1 cut(s) 927
PcsI WCGNNNNNNNCGW 1 cut(s) 484
PctI GAATGC 1 cut(s) 44
PkrI GCNGC 1 cut(s) 566
PleI GAGTC 3 cut(s) 508, 534, 908
PpsI GAGTC 3 cut(s) 508, 534, 908
Ppu21I YACGTR 1 cut(s) 401
PpuMI RGGWCCY 1 cut(s) 817
Psp5II RGGWCCY 1 cut(s) 817
Psp6I CCWGG 1 cut(s) 508
PspGI CCWGG 1 cut(s) 508
PspN4I GGNNCC 1 cut(s) 514
PspPI GGNCC 3 cut(s) 189, 209, 817
PspPPI RGGWCCY 1 cut(s) 817
PsuI RGATCY 1 cut(s) 237
RsaI GTAC 1 cut(s) 282
RsaNI GTAC 1 cut(s) 281
RseI CAYNNNNRTG 1 cut(s) 947
SaqAI TTAA 6 cut(s) 21, 108, 425, 707, 962, 988
SatI GCNGC 1 cut(s) 565
Sau3AI GATC 3 cut(s) 237, 295, 607
Sau96I GGNCC 3 cut(s) 189, 209, 817
ScaI AGTACT 1 cut(s) 282
SchI GAGTC 3 cut(s) 509, 534, 908
ScrFI CCNGG 1 cut(s) 510
SfaNI GCATC 2 cut(s) 618, 912
SinI GGWCC 3 cut(s) 189, 209, 817
SmiMI CAYNNNNRTG 1 cut(s) 947
SmlI CTYRAG 2 cut(s) 416, 632
SmoI CTYRAG 2 cut(s) 416, 632
SnaBI TACGTA 1 cut(s) 401
Sse9I AATT 6 cut(s) 14, 216, 349, 647, 964, 979
SsiI CCGC 1 cut(s) 565
SspMI CTAG 2 cut(s) 161, 264
StyD4I CCNGG 1 cut(s) 508
StyI CCWWGG 4 cut(s) 192, 273, 687, 876
TaaI ACNGT 2 cut(s) 247, 946
TaiI ACGT 1 cut(s) 403
TaqI TCGA 2 cut(s) 745, 762
TasI AATT 6 cut(s) 14, 216, 349, 647, 964, 979
TatI WGTACW 1 cut(s) 280
TauI GCSGC 1 cut(s) 567
Tru1I TTAA 6 cut(s) 21, 108, 425, 707, 962, 988
Tru9I TTAA 6 cut(s) 21, 108, 425, 707, 962, 988
TscAI CASTG 2 cut(s) 361, 913
TspDTI ATGAA 5 cut(s) 246, 731, 927, 962, 965
TspRI CASTG 2 cut(s) 361, 913
VpaK11BI GGWCC 3 cut(s) 189, 209, 817
XagI CCTNNNNNAGG 1 cut(s) 80
XmiI GTMKAC 1 cut(s) 912
XspI CTAG 2 cut(s) 161, 264
ZrmI AGTACT 1 cut(s) 282
Zsp2I ATGCAT 1 cut(s) 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.