Rroxscaffold_3G00263240

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013668
Physical Location & Seq
Forward (+)
57218607 .. 57221306
2700 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00263240.1

Sequence Viewer

Length: 1362 bp
ATGAGGAAGGAGTTATCACTATACAAGTACGGAAGCCTCCAAGACTCTAGAACCTCCCAGCTAGGAAACAAGCCTCACCTATCTCTCCGGTCCTTGGCACAAAAGTATGGTCCAATAATTTACTTACAACTTGGTGAGATCCCAACTGTGGTGGTTTCATCAGCTAGACTTGCCAAGGAAGTACTGAAAACCCATGATCTTGCACTATCAAGCCGTCCACAAATCTTTTCAGCTAAACACCTCTTCTATAATTGCACTGATGTTGCCTTCTCCCCTTATGGTGCTTATTGGAGACACATACGTAAGATTTGCATCCTTGAGCTGTTAAGTGCCAAAAGAGTGCAGTCATTTAGCCATGCCAGAGAAGAAGAAGTTGCTCGTCTGGCTCGTCGGGTTGCAGAGTCCTATCCTGGCACCACTAATCTCAGCAAGTTGCTTGGACTCTATGCAAATGATATTCTCTGCCGCGTAGCATTTGGAAGAGATTTCTCAGGTGGTGGAGAGTATGATCAGCATGATTTCCAAAAGATGCTTGAGGAATATCAGGAATTGCTTGGTGGATTTTCTATTGGAGATTTCTTCCCTTCCATGGAGTTCCTCCACACCTTAACAGGAATGAAATCAAGACTTCAAGACACGTTCGAGAAAAAAGAGGAGCATAAGGACCTCGTGGATGTTTTACTGGAGATACAGAAGGAAGAGACTGACGATATGCCTCTTACCATGGATAATGTCAAGGCTATTATTTTGGACATGTTTGCTGCTGGAACAGACACAACCTTCATCACCCTTGATTGGGGGATGACAGAACTTCTGATGAACCCCAAAGTTCTCAAAAAAGCACAAGCTGAAGTAAGAGGTGTTGTTGGGGAAAAAAGAGTTGTGTTAGAGGGTGATCTTCATCAGCTGGACTATTTGAAAGCTGTCATTAAAGAGATATTTCGGCTGCATCCACCTGCACCAGTACTAGTACCTAGAGAATCCATGGAAGATGTAACCATTGATGGATACCACATTCTAGCCAAAACAAGAATTTTTGTCAATGCCTGGGCAATAGGGAGAGACCCAGAATCTTGGGAAAACCCAGAAAAGTTTGAACCAGAAAGATTTATGGGTAGCACTATTGATTTCAAGGGGCAGGATTTTGAGCTTTTACCTTTTGGGGCGGGTAGAAGAGGTTGTCCAGCTATTACATTCAGCACAGCCAGCATTGAGCTTGCTCTAGCACAACTTCTGCATAGCTTTGATTGGGAGCTTCCTGCTGGTACTGCAGCTGATGATTTGGACATGACAGAAGTTTTTGGCATAACAATGCACAGGATAGCCAATCTGATTGTTGTCGCCAGACCATGGTTTCCCTGA

Protein Analysis

453

Amino Acids

51.33

Weight (kDa)

5.7

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 17 - 438 3.7e-93 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 964
Acc36I ACCTGC 1 cut(s) 964
AccB1I GGYRCC 1 cut(s) 413
AccB7I CCANNNNNTGG 1 cut(s) 1350
AccII CGCG 1 cut(s) 468
AciI CCGC 2 cut(s) 466, 1166
AclWI GGATC 1 cut(s) 133
AcsI RAATTY 1 cut(s) 1032
AcuI CTGAAG 1 cut(s) 870
AfaI GTAC 5 cut(s) 29, 183, 966, 972, 1267
AfiI CCNNNNNNNGG 6 cut(s) 94, 148, 589, 795, 796, 1350
AflIII ACRYGT 2 cut(s) 636, 753
AgsI TTSAA 4 cut(s) 632, 919, 1097, 1132
AhlI ACTAGT 1 cut(s) 967
AjnI CCWGG 2 cut(s) 409, 1046
Alw26I GTCTC 3 cut(s) 286, 695, 1056
AlwI GGATC 1 cut(s) 133
ApeKI GCWGC 3 cut(s) 761, 946, 1271
ApoI RAATTY 1 cut(s) 1032
ArsI GACNNNNNNTTYG 2 cut(s) 94, 126
AspS9I GGNCC 3 cut(s) 90, 110, 664
AsuHPI GGTGA 4 cut(s) 68, 146, 778, 905
AvaII GGWCC 3 cut(s) 90, 110, 664
BanI GGYRCC 1 cut(s) 413
BauI CACGAG 1 cut(s) 668
BbvI GCAGC 3 cut(s) 748, 933, 1283
BccI CCATC 1 cut(s) 998
BceAI ACGGC 1 cut(s) 198
BciT130I CCWGG 2 cut(s) 411, 1048
BciVI GTATCC 1 cut(s) 1001
BclI TGATCA 1 cut(s) 508
BcoDI GTCTC 3 cut(s) 286, 695, 1056
BcuI ACTAGT 1 cut(s) 967
BfaI CTAG 7 cut(s) 48, 62, 165, 968, 975, 1019, 1223
BfmI CTRYAG 1 cut(s) 1269
BfuAI ACCTGC 1 cut(s) 964
BfuI GTATCC 1 cut(s) 1001
BisI GCNGC 4 cut(s) 466, 762, 947, 1272
BlsI GCNGC 4 cut(s) 467, 763, 948, 1273
BmcAI AGTACT 2 cut(s) 183, 966
Bme1390I CCNGG 2 cut(s) 411, 1048
Bme18I GGWCC 3 cut(s) 90, 110, 664
BmgT120I GGNCC 3 cut(s) 90, 110, 664
BmiI GGNNCC 1 cut(s) 415
BmrFI CCNGG 2 cut(s) 411, 1048
BmsI GCATC 3 cut(s) 321, 519, 958
BpmI CTGGAG 1 cut(s) 704
BpuEI CTTGAG 2 cut(s) 338, 554
BsaAI YACGTR 1 cut(s) 302
BsaBI GATNNNNATC 2 cut(s) 311, 900
BsaI GGTCTC 1 cut(s) 1056
BsaJI CCNNGG 7 cut(s) 93, 174, 588, 723, 984, 1047, 1349
BsaWI WCCGGW 1 cut(s) 87
Bsc4I CCNNNNNNNGG 6 cut(s) 94, 148, 589, 795, 796, 1350
Bse1I ACTGG 2 cut(s) 687, 962
Bse8I GATNNNNATC 2 cut(s) 311, 900
BseBI CCWGG 2 cut(s) 411, 1048
BseDI CCNNGG 7 cut(s) 93, 174, 588, 723, 984, 1047, 1349
BseGI GGATG 4 cut(s) 312, 679, 807, 949
BseJI GATNNNNATC 2 cut(s) 311, 900
BseLI CCNNNNNNNGG 6 cut(s) 94, 148, 589, 795, 796, 1350
BseMII CTCAG 2 cut(s) 439, 504
BseNI ACTGG 2 cut(s) 687, 962
BseRI GAGGAG 1 cut(s) 668
BseXI GCAGC 3 cut(s) 748, 933, 1283
BseYI CCCAGC 1 cut(s) 57
BsgI GTGCAG 2 cut(s) 362, 942
Bsh1236I CGCG 1 cut(s) 468
BshNI GGYRCC 1 cut(s) 413
BsiSI CCGG 1 cut(s) 88
BslI CCNNNNNNNGG 6 cut(s) 94, 148, 589, 795, 796, 1350
BsmAI GTCTC 3 cut(s) 286, 695, 1056
Bso31I GGTCTC 1 cut(s) 1056
Bsp143I GATC 4 cut(s) 138, 196, 508, 895
Bsp19I CCATGG 4 cut(s) 588, 723, 984, 1349
BspACI CCGC 2 cut(s) 466, 1166
BspCNI CTCAG 2 cut(s) 438, 503
BspFNI CGCG 1 cut(s) 468
BspLI GGNNCC 1 cut(s) 415
BspMAI CTGCAG 1 cut(s) 1273
BspMI ACCTGC 1 cut(s) 964
BspPI GGATC 1 cut(s) 133
BspT107I GGYRCC 1 cut(s) 413
BspTNI GGTCTC 1 cut(s) 1056
BsrI ACTGG 2 cut(s) 687, 962
BssECI CCNNGG 7 cut(s) 93, 174, 588, 723, 984, 1047, 1349
BssMI GATC 4 cut(s) 138, 196, 508, 895
BssSI CACGAG 1 cut(s) 668
BssT1I CCWWGG 6 cut(s) 93, 174, 588, 723, 984, 1349
Bst2BI CACGAG 1 cut(s) 668
Bst2UI CCWGG 2 cut(s) 411, 1048
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 4 cut(s) 248, 475, 693, 1168
BstBAI YACGTR 1 cut(s) 302
BstC8I GCNNGC 2 cut(s) 1207, 1218
BstDEI CTNAG 2 cut(s) 425, 490
BstDSI CCRYGG 4 cut(s) 588, 723, 984, 1349
BstF5I GGATG 4 cut(s) 312, 679, 807, 949
BstFNI CGCG 1 cut(s) 468
BstKTI GATC 4 cut(s) 141, 199, 511, 898
BstMAI GTCTC 3 cut(s) 286, 695, 1056
BstMBI GATC 4 cut(s) 138, 196, 508, 895
BstMWI GCNNNNNNNGC 4 cut(s) 170, 383, 1206, 1268
BstNI CCWGG 2 cut(s) 411, 1048
BstNSI RCATGY 1 cut(s) 757
BstSCI CCNGG 2 cut(s) 409, 1046
BstSFI CTRYAG 1 cut(s) 1269
BstSNI TACGTA 1 cut(s) 302
BstUI CGCG 1 cut(s) 468
BstV1I GCAGC 3 cut(s) 748, 933, 1283
BstX2I RGATCY 1 cut(s) 138
BstXI CCANNNNNNTGG 1 cut(s) 1074
BstYI RGATCY 1 cut(s) 138
BsuI GTATCC 1 cut(s) 1001
BtgI CCRYGG 4 cut(s) 588, 723, 984, 1349
BtsCI GGATG 4 cut(s) 312, 679, 807, 949
BtsIMutI CAGTG 1 cut(s) 255
BveI ACCTGC 1 cut(s) 964
Cac8I GCNNGC 2 cut(s) 1207, 1218
Cfr13I GGNCC 3 cut(s) 90, 110, 664
Csp6I GTAC 5 cut(s) 28, 182, 965, 971, 1266
CspCI CAANNNNNGTGG 2 cut(s) 132, 167
CviAII CATG 9 cut(s) 194, 356, 515, 589, 724, 754, 985, 1288, 1350
CviQI GTAC 5 cut(s) 28, 182, 965, 971, 1266
DdeI CTNAG 2 cut(s) 425, 490
DpnI GATC 4 cut(s) 140, 198, 510, 897
DpnII GATC 4 cut(s) 138, 196, 508, 895
Eam1104I CTCTTC 4 cut(s) 248, 475, 693, 1168
EarI CTCTTC 4 cut(s) 248, 475, 693, 1168
Eco105I TACGTA 1 cut(s) 302
Eco130I CCWWGG 6 cut(s) 93, 174, 588, 723, 984, 1349
Eco31I GGTCTC 1 cut(s) 1056
Eco47I GGWCC 3 cut(s) 90, 110, 664
Eco57I CTGAAG 1 cut(s) 870
EcoO109I RGGNCCY 1 cut(s) 664
EcoRII CCWGG 2 cut(s) 409, 1046
EcoT14I CCWWGG 6 cut(s) 93, 174, 588, 723, 984, 1349
ErhI CCWWGG 6 cut(s) 93, 174, 588, 723, 984, 1349
FaeI CATG 9 cut(s) 197, 359, 518, 592, 727, 757, 988, 1291, 1353
FatI CATG 9 cut(s) 193, 355, 514, 588, 723, 753, 984, 1287, 1349
FauI CCCGC 1 cut(s) 1159
FbaI TGATCA 1 cut(s) 508
Fnu4HI GCNGC 4 cut(s) 466, 762, 947, 1272
FokI GGATG 4 cut(s) 299, 686, 814, 936
Fsp4HI GCNGC 4 cut(s) 466, 762, 947, 1272
FspBI CTAG 7 cut(s) 48, 62, 165, 968, 975, 1019, 1223
GluI GCNGC 4 cut(s) 466, 762, 947, 1272
GsaI CCCAGC 1 cut(s) 61
GsuI CTGGAG 1 cut(s) 704
HapII CCGG 1 cut(s) 88
Hin1II CATG 9 cut(s) 197, 359, 518, 592, 727, 757, 988, 1291, 1353
HinfI GANTC 5 cut(s) 44, 401, 441, 980, 1070
HpaII CCGG 1 cut(s) 88
HphI GGTGA 4 cut(s) 68, 146, 778, 905
Hpy166II GTNNAC 1 cut(s) 218
Hpy188I TCNGA 2 cut(s) 816, 1332
Hpy188III TCNNGA 5 cut(s) 48, 545, 624, 632, 643
Hpy8I GTNNAC 1 cut(s) 218
Hpy99I CGWCG 1 cut(s) 393
HpyAV CCTTC 4 cut(s) 277, 594, 688, 790
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 2 cut(s) 301, 638
HpyF10VI GCNNNNNNNGC 4 cut(s) 170, 383, 1206, 1268
HpyF3I CTNAG 2 cut(s) 425, 490
HpySE526I ACGT 2 cut(s) 301, 638
Hsp92II CATG 9 cut(s) 197, 359, 518, 592, 727, 757, 988, 1291, 1353
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 4 cut(s) 138, 196, 508, 895
LmnI GCTCC 2 cut(s) 655, 1252
Lsp1109I GCAGC 3 cut(s) 748, 933, 1283
LweI GCATC 3 cut(s) 321, 519, 958
MaeI CTAG 7 cut(s) 48, 62, 165, 968, 975, 1019, 1223
MaeII ACGT 2 cut(s) 301, 638
MaeIII GTNAC 1 cut(s) 994
MalI GATC 4 cut(s) 140, 198, 510, 897
MboI GATC 4 cut(s) 138, 196, 508, 895
MboII GAAGA 9 cut(s) 235, 377, 380, 492, 571, 710, 890, 1001, 1185
MflI RGATCY 1 cut(s) 138
MluCI AATT 4 cut(s) 117, 250, 548, 1032
MlyI GAGTC 3 cut(s) 38, 410, 435
MseI TTAA 3 cut(s) 326, 608, 930
MslI CAYNNNNRTG 1 cut(s) 1310
MspA1I CMGCKG 2 cut(s) 907, 1274
MspI CCGG 1 cut(s) 88
MspR9I CCNGG 2 cut(s) 411, 1048
MvaI CCWGG 2 cut(s) 411, 1048
MvnI CGCG 1 cut(s) 468
MwoI GCNNNNNNNGC 4 cut(s) 170, 383, 1206, 1268
NcoI CCATGG 4 cut(s) 588, 723, 984, 1349
NdeII GATC 4 cut(s) 138, 196, 508, 895
NlaIII CATG 9 cut(s) 197, 359, 518, 592, 727, 757, 988, 1291, 1353
NlaIV GGNNCC 1 cut(s) 415
NspI RCATGY 1 cut(s) 757
PaqCI CACCTGC 1 cut(s) 964
PciI ACATGT 1 cut(s) 753
PcsI WCGNNNNNNNCGW 1 cut(s) 385
PfeI GAWTC 2 cut(s) 980, 1070
PflMI CCANNNNNTGG 1 cut(s) 1350
PkrI GCNGC 4 cut(s) 467, 763, 948, 1273
PleI GAGTC 3 cut(s) 38, 409, 435
PpsI GAGTC 3 cut(s) 38, 409, 435
Ppu21I YACGTR 1 cut(s) 302
PpuMI RGGWCCY 1 cut(s) 664
PscI ACATGT 1 cut(s) 753
Psp5II RGGWCCY 1 cut(s) 664
Psp6I CCWGG 2 cut(s) 409, 1046
PspFI CCCAGC 1 cut(s) 57
PspGI CCWGG 2 cut(s) 409, 1046
PspN4I GGNNCC 1 cut(s) 415
PspPI GGNCC 3 cut(s) 90, 110, 664
PspPPI RGGWCCY 1 cut(s) 664
PstI CTGCAG 1 cut(s) 1273
PsuI RGATCY 1 cut(s) 138
PvuII CAGCTG 2 cut(s) 907, 1274
RsaI GTAC 5 cut(s) 29, 183, 966, 972, 1267
RsaNI GTAC 5 cut(s) 28, 182, 965, 971, 1266
RseI CAYNNNNRTG 1 cut(s) 1310
SaqAI TTAA 3 cut(s) 326, 608, 930
SatI GCNGC 4 cut(s) 466, 762, 947, 1272
Sau3AI GATC 4 cut(s) 138, 196, 508, 895
Sau96I GGNCC 3 cut(s) 90, 110, 664
ScaI AGTACT 2 cut(s) 183, 966
SchI GAGTC 3 cut(s) 38, 410, 435
ScrFI CCNGG 2 cut(s) 411, 1048
SfaNI GCATC 3 cut(s) 321, 519, 958
SfcI CTRYAG 1 cut(s) 1269
SinI GGWCC 3 cut(s) 90, 110, 664
SmiMI CAYNNNNRTG 1 cut(s) 1310
SmlI CTYRAG 2 cut(s) 317, 533
SmoI CTYRAG 2 cut(s) 317, 533
SnaBI TACGTA 1 cut(s) 302
SpeI ACTAGT 1 cut(s) 967
Sse9I AATT 4 cut(s) 117, 250, 548, 1032
SsiI CCGC 2 cut(s) 466, 1166
SspMI CTAG 7 cut(s) 48, 62, 165, 968, 975, 1019, 1223
StyD4I CCNGG 2 cut(s) 409, 1046
StyI CCWWGG 6 cut(s) 93, 174, 588, 723, 984, 1349
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 2 cut(s) 304, 641
TaqI TCGA 1 cut(s) 642
TasI AATT 4 cut(s) 117, 250, 548, 1032
TatI WGTACW 2 cut(s) 181, 964
TauI GCSGC 1 cut(s) 468
TfiI GAWTC 2 cut(s) 980, 1070
Tru1I TTAA 3 cut(s) 326, 608, 930
Tru9I TTAA 3 cut(s) 326, 608, 930
TscAI CASTG 1 cut(s) 262
TseI GCWGC 3 cut(s) 761, 946, 1271
TspDTI ATGAA 5 cut(s) 147, 632, 772, 833, 890
TspGWI ACGGA 1 cut(s) 45
TspRI CASTG 1 cut(s) 262
Van91I CCANNNNNTGG 1 cut(s) 1350
VpaK11BI GGWCC 3 cut(s) 90, 110, 664
XapI RAATTY 1 cut(s) 1032
XbaI TCTAGA 1 cut(s) 47
XceI RCATGY 1 cut(s) 757
XspI CTAG 7 cut(s) 48, 62, 165, 968, 975, 1019, 1223
ZrmI AGTACT 2 cut(s) 183, 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.