Rh7BG125300

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0013668
Physical Location & Seq
Reverse (-)
9305672 .. 9307314
1643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG125300.1

Sequence Viewer

Length: 1515 bp
ATGGCTCTTCTTCAATTTCTAAAGGAACCTTCTGTGCTTTTTGCATTCAGTTTTCTCATAGTGTTGCTCAATTTTATCTTCAGGGATAAGTTGAGGAAAACAAAAATCAAACTCCCACCTAGCCCTCCAAAGCTACCCATCATTGGTAACCTTCACCAGCTTGGAAACAGCCCTCACCTATCTCTCTGGTCCTTGGCAGAAAAGTTTGGTCCCTTAATTTACTTACAACTTGGTCACATCCCAACAGTGGTGGTGTCATCAGCTAAACTAGCCAAGGAAGTACTGAAAACTCATGATCTTGCACTTTCTAGCCGCCCACAGATCTTTTCAGCCAAACATCTCTTCTATAACTGCACTGATGTTGCCTTCTCCCCTTATGGTGCTTATTGGAGGCACATTCGTAAGATTTGCATACTTGAGCTACTAAGTGCCAAAAGAGTGCAATCATTTAGTCATGTAAGAGTAGAAGAAGTTGCTCGTCTGGCTCATCGGGTTGCAGAGTCCTATCCTGGCACCACTAATCTGAGCAAGTTGCTTGGACTATATGCAAATGATATTCTCTGCCGCGTAGCATTTGGAAGAGATTTCTCAGGTGGTGGAGAGTATGATCAGCATGGTTTCCAAAAGATGCTTGAGGAATACCAGGAATTGCTTGGCGGATTTTCTATTGGAGATTTCTTCCCTTCTATGGAGTTTCTGCACACCTTAACAGGAATGAAATCAAGACTTCAAGAGACATTCGGGAGATTCGATAAACTATTTGACCAGATGATAACTGAACATCAAAATCCCAAAAGAGAAAAACAGGAGCACAAGGACCTCGTGGATGTTTTACTGGATATACAGAAAAAAGATTCTGCTGACATGCCTCTCACCATGGATAATGTCAAGGCTATTATCTTGGACATGTTTGCTGCAGGAACTGACACAACCTTCATCACCCTTGATTGGGGAATGACAGAGCTTTTGATGAACCCCAAAGTCCTGGAAAAAGCACAAGCTGAAGTACGAGGTGTTGTTGGGGAAAGAAGAGTCGTATTGGAGAGTGATCTGCATCAGCTGGACTACATGAAAGCTGTCATCAAAGAGATCTATAGGTTGCATCCAGCAGCACCAGTACTTCTCCCTAGAGAATCCATGGAAGATGTAAACATTGATGGGTATGATATTCCAGCCAAAACAAGAATTTTTGTCAATGCCTGGGCAATAGGGAGAGACCCAGAATCTTGGGAAAACCCAGAAATGTTTGAACCAGAAAGGTTTATAGGTAGCACTATTGATTTCAAGGGGCAGGACTTTGAGCTTATACCTTTTGGGGCTGGTAGAAGAGGTTGTCCAGCGATTACATTCAGCACAGCCAGCATTGAGCTTGCTCTAGCACAACTTCTGCATAGCTTTGATTGGGAGCTTCCTGCTGGTACTACAGCTAAAGATTTGGACTTGGCCGAAGCTTTTGGCATCACAATGCATAGGATAGCCAATCTGATTGTTGTAGCCAAACCACGGTTTTCCTGA

Protein Analysis

504

Amino Acids

57.02

Weight (kDa)

6.36

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 39 - 486 1.6e-104 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 512
AccII CGCG 1 cut(s) 567
AciI CCGC 3 cut(s) 313, 565, 657
AcoI YGGCCR 1 cut(s) 1443
AcsI RAATTY 1 cut(s) 1185
AcuI CTGAAG 2 cut(s) 64, 1023
AfaI GTAC 4 cut(s) 282, 1008, 1119, 1420
AfiI CCNNNNNNNGG 6 cut(s) 143, 247, 688, 948, 949, 1503
AflIII ACRYGT 1 cut(s) 906
AgsI TTSAA 4 cut(s) 14, 731, 1250, 1285
AjnI CCWGG 4 cut(s) 508, 642, 984, 1199
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
Alw21I GWGCWC 1 cut(s) 813
Alw26I GTCTC 2 cut(s) 728, 1209
AlwNI CAGNNNCTG 1 cut(s) 923
AoxI GGCC 1 cut(s) 1443
ApeKI GCWGC 2 cut(s) 914, 1109
ApoI RAATTY 1 cut(s) 1185
ArsI GACNNNNNNTTYG 2 cut(s) 949, 981
AspS9I GGNCC 3 cut(s) 189, 209, 817
AsuHPI GGTGA 4 cut(s) 146, 167, 865, 931
AvaII GGWCC 3 cut(s) 189, 209, 817
BanI GGYRCC 1 cut(s) 512
BauI CACGAG 1 cut(s) 821
Bbv12I GWGCWC 1 cut(s) 813
BbvI GCAGC 2 cut(s) 901, 1121
BccI CCATC 2 cut(s) 146, 1151
BciT130I CCWGG 4 cut(s) 510, 644, 986, 1201
BclI TGATCA 1 cut(s) 607
BcoDI GTCTC 2 cut(s) 728, 1209
BfaI CTAG 5 cut(s) 120, 269, 309, 1128, 1376
BfmI CTRYAG 3 cut(s) 915, 1093, 1422
BglII AGATCT 2 cut(s) 321, 1089
BisI GCNGC 4 cut(s) 313, 565, 915, 1110
BlsI GCNGC 4 cut(s) 314, 566, 916, 1111
BmcAI AGTACT 2 cut(s) 282, 1119
Bme1390I CCNGG 4 cut(s) 510, 644, 986, 1201
Bme18I GGWCC 3 cut(s) 189, 209, 817
BmgT120I GGNCC 3 cut(s) 189, 209, 817
BmiI GGNNCC 3 cut(s) 27, 211, 514
BmrFI CCNGG 4 cut(s) 510, 644, 986, 1201
BmsI GCATC 4 cut(s) 618, 1063, 1111, 1467
BpuEI CTTGAG 2 cut(s) 437, 653
BsaBI GATNNNNATC 1 cut(s) 1053
BsaI GGTCTC 1 cut(s) 1209
BsaJI CCNNGG 6 cut(s) 192, 273, 876, 1137, 1200, 1502
Bsc4I CCNNNNNNNGG 6 cut(s) 143, 247, 688, 948, 949, 1503
Bse1I ACTGG 2 cut(s) 840, 1115
Bse8I GATNNNNATC 1 cut(s) 1053
BseBI CCWGG 4 cut(s) 510, 644, 986, 1201
BseDI CCNNGG 6 cut(s) 192, 273, 876, 1137, 1200, 1502
BseGI GGATG 3 cut(s) 237, 832, 1102
BseJI GATNNNNATC 1 cut(s) 1053
BseLI CCNNNNNNNGG 6 cut(s) 143, 247, 688, 948, 949, 1503
BseMII CTCAG 2 cut(s) 515, 603
BseNI ACTGG 2 cut(s) 840, 1115
BseXI GCAGC 2 cut(s) 901, 1121
BsgI GTGCAG 2 cut(s) 337, 683
Bsh1236I CGCG 1 cut(s) 567
BshFI GGCC 1 cut(s) 1445
BshNI GGYRCC 1 cut(s) 512
BsiHKAI GWGCWC 1 cut(s) 813
BslFI GGGAC 1 cut(s) 195
BslI CCNNNNNNNGG 6 cut(s) 143, 247, 688, 948, 949, 1503
BsmAI GTCTC 2 cut(s) 728, 1209
BsmFI GGGAC 1 cut(s) 195
BsmI GAATGC 1 cut(s) 44
BsnI GGCC 1 cut(s) 1445
Bso31I GGTCTC 1 cut(s) 1209
Bsp1286I GDGCHC 1 cut(s) 813
Bsp143I GATC 5 cut(s) 295, 321, 607, 1048, 1089
Bsp19I CCATGG 2 cut(s) 876, 1137
BspACI CCGC 3 cut(s) 313, 565, 657
BspANI GGCC 1 cut(s) 1445
BspCNI CTCAG 2 cut(s) 516, 602
BspFNI CGCG 1 cut(s) 567
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 3 cut(s) 27, 211, 514
BspMAI CTGCAG 1 cut(s) 919
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 512
BspTNI GGTCTC 1 cut(s) 1209
BsrI ACTGG 2 cut(s) 840, 1115
BssECI CCNNGG 6 cut(s) 192, 273, 876, 1137, 1200, 1502
BssMI GATC 5 cut(s) 295, 321, 607, 1048, 1089
BssSI CACGAG 1 cut(s) 821
BssT1I CCWWGG 4 cut(s) 192, 273, 876, 1137
Bst2BI CACGAG 1 cut(s) 821
Bst2UI CCWGG 4 cut(s) 510, 644, 986, 1201
Bst4CI ACNGT 2 cut(s) 247, 1506
Bst6I CTCTTC 5 cut(s) 12, 347, 574, 1024, 1321
BstC8I GCNNGC 2 cut(s) 1360, 1371
BstDEI CTNAG 3 cut(s) 425, 524, 589
BstDSI CCRYGG 3 cut(s) 876, 1137, 1502
BstEII GGTNACC 1 cut(s) 146
BstF5I GGATG 3 cut(s) 237, 832, 1102
BstFNI CGCG 1 cut(s) 567
BstKTI GATC 5 cut(s) 298, 324, 610, 1051, 1092
BstMAI GTCTC 2 cut(s) 728, 1209
BstMBI GATC 5 cut(s) 295, 321, 607, 1048, 1089
BstMWI GCNNNNNNNGC 3 cut(s) 269, 482, 1359
BstNI CCWGG 4 cut(s) 510, 644, 986, 1201
BstNSI RCATGY 2 cut(s) 868, 910
BstPI GGTNACC 1 cut(s) 146
BstSCI CCNGG 4 cut(s) 508, 642, 984, 1199
BstSFI CTRYAG 3 cut(s) 915, 1093, 1422
BstUI CGCG 1 cut(s) 567
BstV1I GCAGC 2 cut(s) 901, 1121
BstX2I RGATCY 2 cut(s) 321, 1089
BstXI CCANNNNNNTGG 2 cut(s) 985, 1227
BstYI RGATCY 2 cut(s) 321, 1089
BsuRI GGCC 1 cut(s) 1445
BtgI CCRYGG 3 cut(s) 876, 1137, 1502
BtsCI GGATG 3 cut(s) 237, 832, 1102
BtsIMutI CAGTG 2 cut(s) 252, 354
Cac8I GCNNGC 2 cut(s) 1360, 1371
CaiI CAGNNNCTG 1 cut(s) 923
CciI TCATGA 1 cut(s) 292
Cfr13I GGNCC 3 cut(s) 189, 209, 817
Csp6I GTAC 4 cut(s) 281, 1007, 1118, 1419
CspCI CAANNNNNGTGG 2 cut(s) 231, 266
CviAII CATG 8 cut(s) 293, 455, 614, 865, 877, 907, 1069, 1138
CviQI GTAC 4 cut(s) 281, 1007, 1118, 1419
DdeI CTNAG 3 cut(s) 425, 524, 589
DpnI GATC 5 cut(s) 297, 323, 609, 1050, 1091
DpnII GATC 5 cut(s) 295, 321, 607, 1048, 1089
EaeI YGGCCR 1 cut(s) 1443
Eam1104I CTCTTC 5 cut(s) 12, 347, 574, 1024, 1321
EarI CTCTTC 5 cut(s) 12, 347, 574, 1024, 1321
EciI GGCGGA 1 cut(s) 672
Eco130I CCWWGG 4 cut(s) 192, 273, 876, 1137
Eco31I GGTCTC 1 cut(s) 1209
Eco47I GGWCC 3 cut(s) 189, 209, 817
Eco57I CTGAAG 2 cut(s) 64, 1023
Eco91I GGTNACC 1 cut(s) 146
EcoO109I RGGNCCY 1 cut(s) 817
EcoO65I GGTNACC 1 cut(s) 146
EcoRII CCWGG 4 cut(s) 508, 642, 984, 1199
EcoT14I CCWWGG 4 cut(s) 192, 273, 876, 1137
EcoT22I ATGCAT 1 cut(s) 1470
ErhI CCWWGG 4 cut(s) 192, 273, 876, 1137
FaeI CATG 8 cut(s) 296, 458, 617, 868, 880, 910, 1072, 1141
FaqI GGGAC 1 cut(s) 195
FatI CATG 8 cut(s) 292, 454, 613, 864, 876, 906, 1068, 1137
FbaI TGATCA 1 cut(s) 607
Fnu4HI GCNGC 4 cut(s) 313, 565, 915, 1110
FokI GGATG 3 cut(s) 224, 839, 1089
Fsp4HI GCNGC 4 cut(s) 313, 565, 915, 1110
FspBI CTAG 5 cut(s) 120, 269, 309, 1128, 1376
GluI GCNGC 4 cut(s) 313, 565, 915, 1110
HaeIII GGCC 1 cut(s) 1445
Hin1II CATG 8 cut(s) 296, 458, 617, 868, 880, 910, 1072, 1141
HindIII AAGCTT 1 cut(s) 1449
HinfI GANTC 6 cut(s) 500, 747, 854, 1032, 1133, 1223
HphI GGTGA 4 cut(s) 146, 167, 865, 931
Hpy166II GTNNAC 1 cut(s) 1150
Hpy188I TCNGA 2 cut(s) 525, 1485
Hpy188III TCNNGA 5 cut(s) 293, 723, 731, 742, 1512
Hpy8I GTNNAC 1 cut(s) 1150
HpyAV CCTTC 5 cut(s) 39, 161, 376, 693, 943
HpyCH4III ACNGT 2 cut(s) 247, 1506
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 482, 1359
HpyF3I CTNAG 3 cut(s) 425, 524, 589
Hsp92II CATG 8 cut(s) 296, 458, 617, 868, 880, 910, 1072, 1141
Ksp22I TGATCA 1 cut(s) 607
Kzo9I GATC 5 cut(s) 295, 321, 607, 1048, 1089
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 2 cut(s) 808, 1405
Lsp1109I GCAGC 2 cut(s) 901, 1121
LweI GCATC 4 cut(s) 618, 1063, 1111, 1467
MaeI CTAG 5 cut(s) 120, 269, 309, 1128, 1376
MaeIII GTNAC 2 cut(s) 146, 233
MalI GATC 5 cut(s) 297, 323, 609, 1050, 1091
MboI GATC 5 cut(s) 295, 321, 607, 1048, 1089
MboII GAAGA 8 cut(s) 70, 334, 479, 591, 670, 1041, 1154, 1338
MflI RGATCY 2 cut(s) 321, 1089
MhlI GDGCHC 1 cut(s) 813
MluCI AATT 5 cut(s) 14, 70, 216, 647, 1185
MlyI GAGTC 2 cut(s) 509, 1041
MnlI CCTC 9 cut(s) 87, 135, 183, 384, 628, 830, 879, 1004, 1322
Mph1103I ATGCAT 1 cut(s) 1470
MseI TTAA 2 cut(s) 215, 707
MslI CAYNNNNRTG 1 cut(s) 1463
MspA1I CMGCKG 1 cut(s) 1060
MspR9I CCNGG 4 cut(s) 510, 644, 986, 1201
Mva1269I GAATGC 1 cut(s) 44
MvaI CCWGG 4 cut(s) 510, 644, 986, 1201
MvnI CGCG 1 cut(s) 567
MwoI GCNNNNNNNGC 3 cut(s) 269, 482, 1359
NcoI CCATGG 2 cut(s) 876, 1137
NdeII GATC 5 cut(s) 295, 321, 607, 1048, 1089
NlaIII CATG 8 cut(s) 296, 458, 617, 868, 880, 910, 1072, 1141
NlaIV GGNNCC 3 cut(s) 27, 211, 514
NmuCI GTSAC 1 cut(s) 233
NsiI ATGCAT 1 cut(s) 1470
NspI RCATGY 2 cut(s) 868, 910
PagI TCATGA 1 cut(s) 292
PciI ACATGT 1 cut(s) 906
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 747
PctI GAATGC 1 cut(s) 44
PfeI GAWTC 4 cut(s) 747, 854, 1133, 1223
PfoI TCCNGGA 1 cut(s) 984
PkrI GCNGC 4 cut(s) 314, 566, 916, 1111
PleI GAGTC 2 cut(s) 508, 1040
PpsI GAGTC 2 cut(s) 508, 1040
PpuMI RGGWCCY 1 cut(s) 817
PscI ACATGT 1 cut(s) 906
Psp5II RGGWCCY 1 cut(s) 817
Psp6I CCWGG 4 cut(s) 508, 642, 984, 1199
PspEI GGTNACC 1 cut(s) 146
PspGI CCWGG 4 cut(s) 508, 642, 984, 1199
PspN4I GGNNCC 3 cut(s) 27, 211, 514
PspPI GGNCC 3 cut(s) 189, 209, 817
PspPPI RGGWCCY 1 cut(s) 817
PstI CTGCAG 1 cut(s) 919
PstNI CAGNNNCTG 1 cut(s) 923
PsuI RGATCY 2 cut(s) 321, 1089
PvuII CAGCTG 1 cut(s) 1060
RsaI GTAC 4 cut(s) 282, 1008, 1119, 1420
RsaNI GTAC 4 cut(s) 281, 1007, 1118, 1419
RseI CAYNNNNRTG 1 cut(s) 1463
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 215, 707
SatI GCNGC 4 cut(s) 313, 565, 915, 1110
Sau3AI GATC 5 cut(s) 295, 321, 607, 1048, 1089
Sau96I GGNCC 3 cut(s) 189, 209, 817
ScaI AGTACT 2 cut(s) 282, 1119
SchI GAGTC 2 cut(s) 509, 1041
ScrFI CCNGG 4 cut(s) 510, 644, 986, 1201
SduI GDGCHC 1 cut(s) 813
SfaNI GCATC 4 cut(s) 618, 1063, 1111, 1467
SfcI CTRYAG 3 cut(s) 915, 1093, 1422
SinI GGWCC 3 cut(s) 189, 209, 817
SmiMI CAYNNNNRTG 1 cut(s) 1463
SmlI CTYRAG 2 cut(s) 416, 632
SmoI CTYRAG 2 cut(s) 416, 632
Sse9I AATT 5 cut(s) 14, 70, 216, 647, 1185
SsiI CCGC 3 cut(s) 313, 565, 657
SspMI CTAG 5 cut(s) 120, 269, 309, 1128, 1376
StyD4I CCNGG 4 cut(s) 508, 642, 984, 1199
StyI CCWWGG 4 cut(s) 192, 273, 876, 1137
TaaI ACNGT 2 cut(s) 247, 1506
TaqI TCGA 1 cut(s) 750
TasI AATT 5 cut(s) 14, 70, 216, 647, 1185
TatI WGTACW 2 cut(s) 280, 1117
TauI GCSGC 2 cut(s) 315, 567
TfiI GAWTC 4 cut(s) 747, 854, 1133, 1223
Tru1I TTAA 2 cut(s) 215, 707
Tru9I TTAA 2 cut(s) 215, 707
TscAI CASTG 2 cut(s) 252, 361
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 2 cut(s) 914, 1109
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 4 cut(s) 731, 925, 986, 1085
TspRI CASTG 2 cut(s) 252, 361
VpaK11BI GGWCC 3 cut(s) 189, 209, 817
XapI RAATTY 1 cut(s) 1185
XceI RCATGY 2 cut(s) 868, 910
XcmI CCANNNNNNNNNTGG 1 cut(s) 650
XspI CTAG 5 cut(s) 120, 269, 309, 1128, 1376
ZrmI AGTACT 2 cut(s) 282, 1119
Zsp2I ATGCAT 1 cut(s) 1470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.