MD14G1175200.v1.1

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013667
Physical Location & Seq
Reverse (-)
26874808 .. 26878023
3216 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1175200.v1.1.491

Sequence Viewer

Length: 1578 bp
ATGAAGACGACGGAGTTTATGGACAAGCAGATCATGGAACTCTCTCGATCCCACTCCGACGATTTCTATCAGCTTTCGTACCCTCAACAATTCGATAACGACGACAAGGACGACGACGACTTCGTTTCCAGCTTTCAGTTCCGCCCCGGACGACGTGTCGTCTCGCAGGAGCCGCGCATTTCCGCTCTCGAGCGTGGCAGTTCTGTGGACCGCCTCAACCTTGCAAAATCTGGAAGCAAGGATGTTGACAGGGCTTCCGAGGATTCGGCATTGATTTCGGTGATAGAGCGAAAGATGGACGAGCATTTTGGCAATCTGTTGCACGGGGTGGAGGGTCTCAGTGCGCGAATTTCTCAGCTGGAGACTCGATTACGCCGGCTGGAGAATTCTGTTGGCGACTTGAAGGATTCTACTGAAATCAACCATGGGAAGGCTGATGGGAAACTGAGGCAACTAGAGAATATGCTAAGAGAGGTTGACAGTGGTATGCAGGATTTGAGAGATAAACAAGAGATAACAGAGGCTCAGTTGGAGCTTGCAAAGCTTCAAATGTTAAAGGGTTACCAGCAATCCGAAAACCAAACGAACAATGTACAAACAGTTTCAGCTCCAGGAGTACTGTCCTCGGCGCCTCGGCAAAGCTACCAACCGCATTCAGTTCAGGCTCCCACCCAACAAGTTCCTTCGCTTCCTACTGATGATCCCCAAACTCTACCCCATCAGAATTTTCCACCGGCACAGGTCCCCACCCAGTCACCTCAGAATCAAATCCCCTCTATTCAATATGCGGATTCTAAGTACTCACCACCTGTGCAAAATCCACAACCCACACCTGAGATGTACTACAGCTTTCAAGTACAGCAGTCGCAACCGCCGCGCACTGCACCGTATCATCTTTCTCCTCCAGCACCACATCCTTCACTAACCTCACAATTACAGCAGCTGCCTCAACAGCAGCCTCCTTTCAATGCTGTTGACCCACAAGCCCAGTTTTCTTTGGTTCATAATCCCGGAGAAACCTCCATGCCATCTCAAAGATATCCTCCAAGCTTCCACAACACCTCTAATGCACCTGGCATGGCTCTCCCAACCCAACAACAGCAATATGCGGGTTCCATTCAGTATACACATGATCAAACCGCAAGCAATCAGTACCCAGAGTTTTCCCCTGGCCATATGCAGCCAGTTCAACATTCATATTTTGATGATTTTCGTTCCCACAGTGGATCTCAGTCGCACAACAGCAGTCCAAACATGAAACACTCGCAAGTCTTGCCAGCCTCATTAGACCGTTCTGGTGGAAGCAGCTTTTCGAAACTGCCAACTGCCAAGGTATTGCCGCACGCAATACCCATGGCTGCTAGTGTGGATAAGGAATCGAGTTCCAGTGGGACTGGAAACAGAATACCAATTGATAATGTCATTGATAAGGTTGTAGCGATGGGATTCCGAAGGGACATGGTCAGAGCAACGGTGAGGAAAATGACTGAGAATGGGCAGTCGGTGGACGTCAATCTTGTGCTGGATAAGCTGATGAACAATGGAGAACAGCCTCAAAGTGGTGGATTTGGCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1421

Protein Analysis

526

Amino Acids

58.38

Weight (kDa)

5.86

Isoelectric Point (pI)

60.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 471 - 515 2.7e-19 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013667)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05870 FvH4_5g05870
malus_domestica MD06G1169700.v1.1 MD14G1175200.v1.1
prunus_persica Prupe.5G168500_v2.0.a1
pyrus_communis pycom06g15100 pycom14g14620
rosa_chinensis RchiOBHm_Chr7g0192951
rosa_laevigata RLG00000004309
rosa_multiflora Rmu_sc0002040.1_g000061
rosa_roxburghii Rroxscaffold_3G00262510
rosa_rugosa Rorug07G0006400 Rorug07G0294800
rosa_samantha Rh7AG131600 Rh7BG132500 Rh7CG135700 Rh7DG133600
rosa_wichuraiana Rw7G011230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1512
AccB1I GGYRCC 1 cut(s) 628
AccBSI CCGCTC 1 cut(s) 185
AccI GTMKAC 1 cut(s) 1124
AccII CGCG 3 cut(s) 175, 346, 877
AclWI GGATC 3 cut(s) 42, 695, 1234
AcoI YGGCCR 2 cut(s) 1171, 1570
AcsI RAATTY 3 cut(s) 348, 385, 724
AcyI GRCGYC 2 cut(s) 629, 1509
AdeI CACNNNGTG 1 cut(s) 328
AfaI GTAC 7 cut(s) 80, 594, 618, 800, 842, 858, 1154
AfiI CCNNNNNNNGG 2 cut(s) 430, 1559
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 6 cut(s) 403, 548, 782, 854, 967, 1190
AhdI GACNNNNNGTC 2 cut(s) 155, 158
AjiI CACGTC 1 cut(s) 155
AjnI CCWGG 3 cut(s) 610, 1072, 1168
Alw26I GTCTC 3 cut(s) 166, 341, 356
AlwI GGATC 3 cut(s) 42, 695, 1234
AlwNI CAGNNNCTG 1 cut(s) 943
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 2 cut(s) 1171, 1570
ApeKI GCWGC 6 cut(s) 940, 943, 955, 1180, 1305, 1358
ApoI RAATTY 3 cut(s) 348, 385, 724
ArsI GACNNNNNNTTYG 4 cut(s) 104, 136, 290, 322
AspLEI GCGC 4 cut(s) 177, 346, 631, 879
AspS9I GGNCC 2 cut(s) 208, 742
AsuC2I CCSGG 2 cut(s) 147, 1011
AsuHPI GGTGA 4 cut(s) 292, 747, 795, 1486
AsuII TTCGAA 1 cut(s) 1313
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 2 cut(s) 208, 742
BalI TGGCCA 1 cut(s) 1173
BanI GGYRCC 1 cut(s) 628
BbsI GAAGAC 1 cut(s) 11
BbvI GCAGC 6 cut(s) 930, 952, 967, 1192, 1317, 1345
BccI CCATC 5 cut(s) 289, 431, 726, 1036, 1435
BcgI CGANNNNNNTGC 2 cut(s) 258, 292
BciT130I CCWGG 3 cut(s) 612, 1074, 1170
BclI TGATCA 1 cut(s) 1132
BcnI CCSGG 2 cut(s) 147, 1011
BcoDI GTCTC 3 cut(s) 166, 341, 356
BfaI CTAG 2 cut(s) 455, 1362
BfmI CTRYAG 1 cut(s) 844
BfoI RGCGCY 1 cut(s) 632
BisI GCNGC 9 cut(s) 173, 875, 941, 944, 956, 1181, 1306, 1340, 1359
BlsI GCNGC 9 cut(s) 174, 876, 942, 945, 957, 1182, 1307, 1341, 1360
BmcAI AGTACT 2 cut(s) 618, 800
Bme1390I CCNGG 5 cut(s) 147, 612, 1011, 1074, 1170
Bme18I GGWCC 2 cut(s) 208, 742
BmeRI GACNNNNNGTC 2 cut(s) 155, 158
BmeT110I CYCGRG 1 cut(s) 188
BmgBI CACGTC 1 cut(s) 155
BmgT120I GGNCC 2 cut(s) 208, 742
BmiI GGNNCC 5 cut(s) 171, 630, 666, 744, 1114
BmrFI CCNGG 5 cut(s) 147, 612, 1011, 1074, 1170
BmrI ACTGGG 2 cut(s) 745, 982
BmuI ACTGGG 2 cut(s) 745, 982
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 4 cut(s) 380, 401, 594, 888
Bpu14I TTCGAA 1 cut(s) 1313
BpuMI CCSGG 2 cut(s) 147, 1011
BsaBI GATNNNNATC 1 cut(s) 66
BsaHI GRCGYC 2 cut(s) 629, 1509
BsaI GGTCTC 1 cut(s) 341
BsaJI CCNNGG 8 cut(s) 145, 258, 424, 624, 632, 1168, 1329, 1353
Bsc4I CCNNNNNNNGG 2 cut(s) 430, 1559
Bse118I RCCGGY 3 cut(s) 375, 733, 1572
Bse1I ACTGG 5 cut(s) 751, 988, 1184, 1386, 1399
Bse8I GATNNNNATC 1 cut(s) 66
BseBI CCWGG 3 cut(s) 612, 1074, 1170
BseDI CCNNGG 8 cut(s) 145, 258, 424, 624, 632, 1168, 1329, 1353
BseGI GGATG 2 cut(s) 247, 913
BseJI GATNNNNATC 1 cut(s) 66
BseLI CCNNNNNNNGG 2 cut(s) 430, 1559
BseMII CTCAG 8 cut(s) 352, 368, 437, 539, 773, 825, 1244, 1479
BseNI ACTGG 5 cut(s) 751, 988, 1184, 1386, 1399
BseRI GAGGAG 1 cut(s) 891
BseXI GCAGC 6 cut(s) 930, 952, 967, 1192, 1317, 1345
BsgI GTGCAG 1 cut(s) 867
Bsh1236I CGCG 3 cut(s) 175, 346, 877
BshFI GGCC 2 cut(s) 1173, 1572
BshNI GGYRCC 1 cut(s) 628
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 5 cut(s) 147, 376, 734, 1011, 1573
BslFI GGGAC 3 cut(s) 728, 1405, 1469
BslI CCNNNNNNNGG 2 cut(s) 430, 1559
BsmAI GTCTC 3 cut(s) 166, 341, 356
BsmBI CGTCTC 1 cut(s) 166
BsmFI GGGAC 3 cut(s) 728, 1405, 1469
BsmI GAATGC 1 cut(s) 652
BsnI GGCC 2 cut(s) 1173, 1572
Bso31I GGTCTC 1 cut(s) 341
BsoBI CYCGRG 1 cut(s) 188
Bsp119I TTCGAA 1 cut(s) 1313
Bsp1407I TGTACA 1 cut(s) 592
Bsp143I GATC 5 cut(s) 30, 47, 700, 1132, 1226
Bsp19I CCATGG 2 cut(s) 424, 1353
BspANI GGCC 2 cut(s) 1173, 1572
BspCNI CTCAG 8 cut(s) 351, 367, 438, 538, 772, 826, 1243, 1480
BspFNI CGCG 3 cut(s) 175, 346, 877
BspLI GGNNCC 5 cut(s) 171, 630, 666, 744, 1114
BspPI GGATC 3 cut(s) 42, 695, 1234
BspT104I TTCGAA 1 cut(s) 1313
BspT107I GGYRCC 1 cut(s) 628
BspTNI GGTCTC 1 cut(s) 341
BsrBI CCGCTC 1 cut(s) 185
BsrFI RCCGGY 3 cut(s) 375, 733, 1572
BsrGI TGTACA 1 cut(s) 592
BsrI ACTGG 5 cut(s) 751, 988, 1184, 1386, 1399
BssAI RCCGGY 3 cut(s) 375, 733, 1572
BssECI CCNNGG 8 cut(s) 145, 258, 424, 624, 632, 1168, 1329, 1353
BssMI GATC 5 cut(s) 30, 47, 700, 1132, 1226
BssNAI GTATAC 1 cut(s) 1125
BssNI GRCGYC 2 cut(s) 629, 1509
BssT1I CCWWGG 3 cut(s) 424, 1329, 1353
Bst1107I GTATAC 1 cut(s) 1125
Bst2UI CCWGG 3 cut(s) 612, 1074, 1170
Bst4CI ACNGT 7 cut(s) 482, 601, 621, 888, 1223, 1292, 1474
BstACI GRCGYC 2 cut(s) 629, 1509
BstAPI GCANNNNNTGC 1 cut(s) 1273
BstAUI TGTACA 1 cut(s) 592
BstBI TTCGAA 1 cut(s) 1313
BstC8I GCNNGC 5 cut(s) 377, 537, 1144, 1278, 1344
BstDSI CCRYGG 2 cut(s) 424, 1353
BstEII GGTNACC 1 cut(s) 560
BstF5I GGATG 2 cut(s) 247, 913
BstFNI CGCG 3 cut(s) 175, 346, 877
BstH2I RGCGCY 1 cut(s) 632
BstHHI GCGC 4 cut(s) 177, 346, 631, 879
BstKTI GATC 5 cut(s) 33, 50, 703, 1135, 1229
BstMAI GTCTC 3 cut(s) 166, 341, 356
BstMBI GATC 5 cut(s) 30, 47, 700, 1132, 1226
BstMWI GCNNNNNNNGC 6 cut(s) 172, 541, 874, 952, 1273, 1528
BstNI CCWGG 3 cut(s) 612, 1074, 1170
BstPI GGTNACC 1 cut(s) 560
BstSCI CCNGG 5 cut(s) 145, 610, 1009, 1072, 1168
BstSFI CTRYAG 1 cut(s) 844
BstUI CGCG 3 cut(s) 175, 346, 877
BstV1I GCAGC 6 cut(s) 930, 952, 967, 1192, 1317, 1345
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 1226
BstYI RGATCY 1 cut(s) 1226
BstZ17I GTATAC 1 cut(s) 1125
BsuRI GGCC 2 cut(s) 1173, 1572
BtgI CCRYGG 2 cut(s) 424, 1353
BtgZI GCGATG 1 cut(s) 1454
BtrI CACGTC 1 cut(s) 155
BtsCI GGATG 2 cut(s) 247, 913
BtsI GCAGTG 1 cut(s) 879
BtsIMutI CAGTG 5 cut(s) 346, 487, 879, 1228, 1393
Cac8I GCNNGC 5 cut(s) 377, 537, 1144, 1278, 1344
CaiI CAGNNNCTG 1 cut(s) 943
CfoI GCGC 4 cut(s) 177, 346, 631, 879
Cfr10I RCCGGY 3 cut(s) 375, 733, 1572
Cfr13I GGNCC 2 cut(s) 208, 742
Csp6I GTAC 7 cut(s) 79, 593, 617, 799, 841, 857, 1153
CviAII CATG 8 cut(s) 34, 425, 1024, 1078, 1130, 1255, 1354, 1459
CviQI GTAC 7 cut(s) 79, 593, 617, 799, 841, 857, 1153
DinI GGCGCC 1 cut(s) 630
DpnI GATC 5 cut(s) 32, 49, 702, 1134, 1228
DpnII GATC 5 cut(s) 30, 47, 700, 1132, 1226
DraIII CACNNNGTG 1 cut(s) 328
DriI GACNNNNNGTC 2 cut(s) 155, 158
EaeI YGGCCR 2 cut(s) 1171, 1570
Eam1105I GACNNNNNGTC 2 cut(s) 155, 158
EciI GGCGGA 1 cut(s) 131
Eco130I CCWWGG 3 cut(s) 424, 1329, 1353
Eco31I GGTCTC 1 cut(s) 341
Eco32I GATATC 1 cut(s) 1040
Eco47I GGWCC 2 cut(s) 208, 742
Eco88I CYCGRG 1 cut(s) 188
Eco91I GGTNACC 1 cut(s) 560
EcoO109I RGGNCCY 1 cut(s) 742
EcoO65I GGTNACC 1 cut(s) 560
EcoRI GAATTC 1 cut(s) 385
EcoRII CCWGG 3 cut(s) 610, 1072, 1168
EcoRV GATATC 1 cut(s) 1040
EcoT14I CCWWGG 3 cut(s) 424, 1329, 1353
EgeI GGCGCC 1 cut(s) 630
EheI GGCGCC 1 cut(s) 630
ErhI CCWWGG 3 cut(s) 424, 1329, 1353
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 8 cut(s) 37, 428, 1027, 1081, 1133, 1258, 1357, 1462
FaqI GGGAC 3 cut(s) 728, 1405, 1469
FatI CATG 8 cut(s) 33, 424, 1023, 1077, 1129, 1254, 1353, 1458
FauI CCCGC 1 cut(s) 1102
FauNDI CATATG 1 cut(s) 1176
FbaI TGATCA 1 cut(s) 1132
FblI GTMKAC 1 cut(s) 1124
Fnu4HI GCNGC 9 cut(s) 173, 875, 941, 944, 956, 1181, 1306, 1340, 1359
FokI GGATG 2 cut(s) 254, 900
Fsp4HI GCNGC 9 cut(s) 173, 875, 941, 944, 956, 1181, 1306, 1340, 1359
FspBI CTAG 2 cut(s) 455, 1362
GlaI GCGC 4 cut(s) 176, 345, 630, 878
GluI GCNGC 9 cut(s) 173, 875, 941, 944, 956, 1181, 1306, 1340, 1359
GsuI CTGGAG 4 cut(s) 380, 401, 594, 888
HaeII RGCGCY 1 cut(s) 632
HaeIII GGCC 2 cut(s) 1173, 1572
HapII CCGG 5 cut(s) 147, 376, 734, 1011, 1573
HhaI GCGC 4 cut(s) 177, 346, 631, 879
Hin1I GRCGYC 2 cut(s) 629, 1509
Hin1II CATG 8 cut(s) 37, 428, 1027, 1081, 1133, 1258, 1357, 1462
Hin6I GCGC 4 cut(s) 175, 344, 629, 877
HinP1I GCGC 4 cut(s) 175, 344, 629, 877
HincII GTYRAC 3 cut(s) 247, 478, 976
HindII GTYRAC 3 cut(s) 247, 478, 976
HindIII AAGCTT 2 cut(s) 542, 1048
HinfI GANTC 7 cut(s) 263, 364, 407, 763, 791, 1376, 1446
HpaII CCGG 5 cut(s) 147, 376, 734, 1011, 1573
HphI GGTGA 4 cut(s) 292, 747, 795, 1486
Hpy166II GTNNAC 6 cut(s) 208, 247, 478, 976, 1125, 1507
Hpy188I TCNGA 7 cut(s) 58, 259, 574, 723, 762, 1451, 1466
Hpy188III TCNNGA 3 cut(s) 45, 188, 231
Hpy8I GTNNAC 6 cut(s) 208, 247, 478, 976, 1125, 1507
Hpy99I CGWCG 6 cut(s) 13, 62, 104, 116, 119, 156
HpyAV CCTTC 5 cut(s) 397, 424, 693, 927, 1446
HpyCH4III ACNGT 7 cut(s) 482, 601, 621, 888, 1223, 1292, 1474
HpyCH4IV ACGT 2 cut(s) 154, 1509
HpyCH4V TGCA 8 cut(s) 224, 322, 490, 539, 814, 884, 1070, 1180
HpyF10VI GCNNNNNNNGC 6 cut(s) 172, 541, 874, 952, 1273, 1528
HpySE526I ACGT 2 cut(s) 154, 1509
Hsp92I GRCGYC 2 cut(s) 629, 1509
Hsp92II CATG 8 cut(s) 37, 428, 1027, 1081, 1133, 1258, 1357, 1462
HspAI GCGC 4 cut(s) 175, 344, 629, 877
KasI GGCGCC 1 cut(s) 628
KroI GCCGGC 1 cut(s) 375
KroNI GCCGGC 1 cut(s) 377
Ksp22I TGATCA 1 cut(s) 1132
Kzo9I GATC 5 cut(s) 30, 47, 700, 1132, 1226
LmnI GCTCC 4 cut(s) 169, 532, 613, 670
Lsp1109I GCAGC 6 cut(s) 930, 952, 967, 1192, 1317, 1345
MaeI CTAG 2 cut(s) 455, 1362
MaeII ACGT 2 cut(s) 154, 1509
MaeIII GTNAC 2 cut(s) 560, 753
MalI GATC 5 cut(s) 32, 49, 702, 1134, 1228
MbiI CCGCTC 1 cut(s) 185
MboI GATC 5 cut(s) 30, 47, 700, 1132, 1226
MboII GAAGA 1 cut(s) 16
MfeI CAATTG 1 cut(s) 1410
MflI RGATCY 1 cut(s) 1226
MlsI TGGCCA 1 cut(s) 1173
MluCI AATT 6 cut(s) 89, 348, 385, 724, 932, 1410
MluNI TGGCCA 1 cut(s) 1173
Mly113I GGCGCC 1 cut(s) 629
MlyI GAGTC 1 cut(s) 358
MmeI TCCRAC 2 cut(s) 81, 510
Mox20I TGGCCA 1 cut(s) 1173
MroNI GCCGGC 1 cut(s) 375
MscI TGGCCA 1 cut(s) 1173
MseI TTAA 1 cut(s) 554
Msp20I TGGCCA 1 cut(s) 1173
MspA1I CMGCKG 2 cut(s) 358, 943
MspI CCGG 5 cut(s) 147, 376, 734, 1011, 1573
MspR9I CCNGG 5 cut(s) 147, 612, 1011, 1074, 1170
MunI CAATTG 1 cut(s) 1410
Mva1269I GAATGC 1 cut(s) 652
MvaI CCWGG 3 cut(s) 612, 1074, 1170
MvnI CGCG 3 cut(s) 175, 346, 877
MwoI GCNNNNNNNGC 6 cut(s) 172, 541, 874, 952, 1273, 1528
NaeI GCCGGC 1 cut(s) 377
NarI GGCGCC 1 cut(s) 629
NciI CCSGG 2 cut(s) 147, 1011
NcoI CCATGG 2 cut(s) 424, 1353
NdeI CATATG 1 cut(s) 1176
NdeII GATC 5 cut(s) 30, 47, 700, 1132, 1226
NgoMIV GCCGGC 1 cut(s) 375
NlaIII CATG 8 cut(s) 37, 428, 1027, 1081, 1133, 1258, 1357, 1462
NlaIV GGNNCC 5 cut(s) 171, 630, 666, 744, 1114
NmeAIII GCCGAG 2 cut(s) 605, 613
NmuCI GTSAC 1 cut(s) 753
NspV TTCGAA 1 cut(s) 1313
PaeR7I CTCGAG 1 cut(s) 188
PcsI WCGNNNNNNNCGW 3 cut(s) 99, 108, 120
PctI GAATGC 1 cut(s) 652
PdiI GCCGGC 1 cut(s) 377
PfeI GAWTC 6 cut(s) 263, 407, 763, 791, 1376, 1446
PflFI GACNNNGTC 1 cut(s) 1460
PfoI TCCNGGA 2 cut(s) 610, 1009
PkrI GCNGC 9 cut(s) 174, 876, 942, 945, 957, 1182, 1307, 1341, 1360
PleI GAGTC 1 cut(s) 358
PluTI GGCGCC 1 cut(s) 632
PpsI GAGTC 1 cut(s) 358
PpuMI RGGWCCY 1 cut(s) 742
Psp5II RGGWCCY 1 cut(s) 742
Psp6I CCWGG 3 cut(s) 610, 1072, 1168
PspEI GGTNACC 1 cut(s) 560
PspGI CCWGG 3 cut(s) 610, 1072, 1168
PspN4I GGNNCC 5 cut(s) 171, 630, 666, 744, 1114
PspPI GGNCC 2 cut(s) 208, 742
PspPPI RGGWCCY 1 cut(s) 742
PstNI CAGNNNCTG 1 cut(s) 943
PsuI RGATCY 1 cut(s) 1226
PsyI GACNNNGTC 1 cut(s) 1460
PvuII CAGCTG 2 cut(s) 358, 943
RsaI GTAC 7 cut(s) 80, 594, 618, 800, 842, 858, 1154
RsaNI GTAC 7 cut(s) 79, 593, 617, 799, 841, 857, 1153
SaqAI TTAA 1 cut(s) 554
SatI GCNGC 9 cut(s) 173, 875, 941, 944, 956, 1181, 1306, 1340, 1359
Sau3AI GATC 5 cut(s) 30, 47, 700, 1132, 1226
Sau96I GGNCC 2 cut(s) 208, 742
ScaI AGTACT 2 cut(s) 618, 800
SchI GAGTC 1 cut(s) 358
ScrFI CCNGG 5 cut(s) 147, 612, 1011, 1074, 1170
SfcI CTRYAG 1 cut(s) 844
SfoI GGCGCC 1 cut(s) 630
Sfr274I CTCGAG 1 cut(s) 188
SfuI TTCGAA 1 cut(s) 1313
SinI GGWCC 2 cut(s) 208, 742
SlaI CTCGAG 1 cut(s) 188
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 6 cut(s) 89, 348, 385, 724, 932, 1410
SspDI GGCGCC 1 cut(s) 628
SspMI CTAG 2 cut(s) 455, 1362
StyD4I CCNGG 5 cut(s) 145, 610, 1009, 1072, 1168
StyI CCWWGG 3 cut(s) 424, 1329, 1353
TaaI ACNGT 7 cut(s) 482, 601, 621, 888, 1223, 1292, 1474
TaiI ACGT 2 cut(s) 157, 1512
TaqI TCGA 6 cut(s) 46, 93, 189, 367, 1313, 1379
TasI AATT 6 cut(s) 89, 348, 385, 724, 932, 1410
TatI WGTACW 5 cut(s) 592, 616, 798, 840, 856
TauI GCSGC 3 cut(s) 175, 877, 1342
TfiI GAWTC 6 cut(s) 263, 407, 763, 791, 1376, 1446
Tru1I TTAA 1 cut(s) 554
Tru9I TTAA 1 cut(s) 554
TscAI CASTG 5 cut(s) 346, 487, 886, 1228, 1393
TseFI GTSAC 1 cut(s) 753
TseI GCWGC 6 cut(s) 940, 943, 955, 1180, 1305, 1358
Tsp45I GTSAC 1 cut(s) 753
TspDTI ATGAA 5 cut(s) 17, 992, 1185, 1271, 1550
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 5 cut(s) 346, 487, 886, 1228, 1393
Tth111I GACNNNGTC 1 cut(s) 1460
VpaK11BI GGWCC 2 cut(s) 208, 742
XapI RAATTY 3 cut(s) 348, 385, 724
XhoI CTCGAG 1 cut(s) 188
XmiI GTMKAC 1 cut(s) 1124
XspI CTAG 2 cut(s) 455, 1362
ZraI GACGTC 1 cut(s) 1510
ZrmI AGTACT 2 cut(s) 618, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.