Rh7AG131600

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0013667
Physical Location & Seq
Reverse (-)
10539318 .. 10542291
2974 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG131600.1

Sequence Viewer

Length: 1191 bp
ATGACAATCTTCTTGACAGGCTTCATGTTTGTAAACAAACCACAGGTTGAAGGTGGTATGCAGGATTTGAGAGATAAGCAAGAGATCTCAGAGGCTCAGCTGCAGCTTGCAAAACTTCAAATGCTTAAGGATCACCAGAAGTTGGAACAAGAAAGGAGCAGTGTGCAAACAACTTCCGCGTCATCTGCTTCTCAGCAATCCCACCAATCATATTCAACTCCTGTTGCTCCTCATCAATCCCACCAATCATATTCAACTCCTGTTGCTTCTGTACAACATCCTTCCCTTCCTCCTGATATTCCCCCAACCCGGACCCATCAGAACTTTCCACCAGCACCTACAGCAGCTCAGCTTCCAGCTCAGCCATCTCAGTATCAAACTCCTTATAGTCAACAGCCAGATTCTAACTACTCGGCACCTGTGTTGAATCCTCCACCCACACCTCAGGCATACTATATGTCTCAAGTTCAGCAGTCGCAACCACCTCCCACAGCACCGTATCAACCCTATCCAGCAGTACCACATCCTTCACAAACATCACATTTTCAGCAGCAGAGTCAACTTCACCCGCCTCTCAATGCTCCTGATCCACAAGCCCAGTATTCATCGATTCACAATCCAGCAGAAACCCCGTATGGGTTATCTCAAAGCTATCCCCAGAGCATCCACAAAACCTCTAATGCGCCTGGCGTGGATCTACCATCACAACAACAGTTTGAGGGTTCCATGCAGTATACACATGACCGATCTACTAGAAATTACAGCACAGACTTCTCCCCTGGAAATATGCAACCAGTTCAACGTTCAAGCTACGATGACTTTTATTCCCACGGCGGATCTCCTTCTCACTACAGTGATTCAAAAATGAAACAGTCGCAAGTATCGCCATCCTCTTCGGTCGCTTCTGGTGGAAGCAGTTTCTCAAGACTGCCAACTGCAAAGGTATTGCCGTATGCATTACCCATGGCTGCCAGTGTGGATAAGGAGTCAGATTCTGGCGGGACTGGAAACCGCGTTCCAGTTGATAATGTCATTGATAAGGTTGTAGCTATGGGATTCCGAAGGGACATAGTCAGAGCAACAGTGAGGAAAATGGCTGAGAATGGGCAGGCAGTGGACCTTAATGTTGTGCTGGATAAACTGATGAACAGTGGAGAAGTTCAGTCCCAAAGTGGTGGATTTGGCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1421

Protein Analysis

396

Amino Acids

43.21

Weight (kDa)

6.38

Isoelectric Point (pI)

76.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 341 - 384 1.6e-19 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013667)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05870 FvH4_5g05870
malus_domestica MD06G1169700.v1.1 MD14G1175200.v1.1
prunus_persica Prupe.5G168500_v2.0.a1
pyrus_communis pycom06g15100 pycom14g14620
rosa_chinensis RchiOBHm_Chr7g0192951
rosa_laevigata RLG00000004309
rosa_multiflora Rmu_sc0002040.1_g000061
rosa_roxburghii Rroxscaffold_3G00262510
rosa_rugosa Rorug07G0006400 Rorug07G0294800
rosa_samantha Rh7AG131600 Rh7BG132500 Rh7CG135700 Rh7DG133600
rosa_wichuraiana Rw7G011230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 415
AccB7I CCANNNNNTGG 1 cut(s) 142
AccI GTMKAC 1 cut(s) 734
AccII CGCG 2 cut(s) 179, 1014
AciI CCGC 5 cut(s) 177, 569, 834, 999, 1012
AclI AACGTT 1 cut(s) 802
AclWI GGATC 4 cut(s) 138, 581, 702, 844
AcoI YGGCCR 1 cut(s) 1183
AfaI GTAC 2 cut(s) 273, 519
AfiI CCNNNNNNNGG 3 cut(s) 142, 309, 636
AflII CTTAAG 1 cut(s) 125
AgsI TTSAA 8 cut(s) 50, 119, 216, 255, 427, 800, 807, 861
AjnI CCWGG 2 cut(s) 685, 778
AleI CACNNNNGTG 1 cut(s) 852
AloI GAACNNNNNNTCC 2 cut(s) 999, 1031
AluBI AGCT 8 cut(s) 100, 106, 347, 352, 359, 651, 810, 1049
AluI AGCT 8 cut(s) 100, 106, 347, 352, 359, 651, 810, 1049
Alw26I GTCTC 1 cut(s) 465
AlwI GGATC 4 cut(s) 138, 581, 702, 844
AlwNI CAGNNNCTG 1 cut(s) 995
AoxI GGCC 1 cut(s) 1183
ApeKI GCWGC 5 cut(s) 100, 103, 344, 550, 968
AspLEI GCGC 1 cut(s) 685
AspS9I GGNCC 2 cut(s) 312, 1117
AsuC2I CCSGG 1 cut(s) 310
AsuHPI GGTGA 2 cut(s) 125, 557
AvaII GGWCC 2 cut(s) 312, 1117
AxyI CCTNAGG 1 cut(s) 444
BalI TGGCCA 1 cut(s) 1185
BanI GGYRCC 1 cut(s) 415
BbvI GCAGC 5 cut(s) 87, 115, 356, 562, 955
BccI CCATC 4 cut(s) 324, 373, 709, 895
BceAI ACGGC 2 cut(s) 847, 934
BciT130I CCWGG 2 cut(s) 687, 780
BcnI CCSGG 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 465
BfaI CTAG 1 cut(s) 753
BfmI CTRYAG 3 cut(s) 101, 339, 850
BfrI CTTAAG 1 cut(s) 125
BglII AGATCT 1 cut(s) 84
BisI GCNGC 5 cut(s) 101, 104, 345, 551, 969
BlpI GCTNAGC 3 cut(s) 96, 348, 360
BlsI GCNGC 5 cut(s) 102, 105, 346, 552, 970
Bme1390I CCNGG 3 cut(s) 310, 687, 780
Bme18I GGWCC 2 cut(s) 312, 1117
BmgT120I GGNCC 2 cut(s) 312, 1117
BmiI GGNNCC 3 cut(s) 314, 417, 724
BmrFI CCNGG 3 cut(s) 310, 687, 780
BmrI ACTGGG 1 cut(s) 592
BmsI GCATC 1 cut(s) 672
BmuI ACTGGG 1 cut(s) 592
Bpu1102I GCTNAGC 3 cut(s) 96, 348, 360
BpuEI CTTGAG 2 cut(s) 447, 907
BpuMI CCSGG 1 cut(s) 310
Bsa29I ATCGAT 1 cut(s) 608
BsaJI CCNNGG 3 cut(s) 778, 829, 963
Bsc4I CCNNNNNNNGG 3 cut(s) 142, 309, 636
Bse1I ACTGG 6 cut(s) 598, 794, 972, 1009, 1019, 1186
Bse21I CCTNAGG 1 cut(s) 444
BseBI CCWGG 2 cut(s) 687, 780
BseCI ATCGAT 1 cut(s) 608
BseDI CCNNGG 3 cut(s) 778, 829, 963
BseGI GGATG 4 cut(s) 277, 523, 663, 887
BseLI CCNNNNNNNGG 3 cut(s) 142, 309, 636
BseMII CTCAG 8 cut(s) 102, 110, 206, 362, 374, 383, 458, 1089
BseNI ACTGG 6 cut(s) 598, 794, 972, 1009, 1019, 1186
BseRI GAGGAG 1 cut(s) 219
BseXI GCAGC 5 cut(s) 87, 115, 356, 562, 955
Bsh1236I CGCG 2 cut(s) 179, 1014
Bsh1285I CGRYCG 1 cut(s) 900
BshFI GGCC 1 cut(s) 1185
BshNI GGYRCC 1 cut(s) 415
BshVI ATCGAT 1 cut(s) 608
BsiEI CGRYCG 1 cut(s) 900
BsiSI CCGG 1 cut(s) 310
BslFI GGGAC 3 cut(s) 1015, 1079, 1150
BslI CCNNNNNNNGG 3 cut(s) 142, 309, 636
BsmAI GTCTC 1 cut(s) 465
BsmFI GGGAC 3 cut(s) 1015, 1079, 1150
BsnI GGCC 1 cut(s) 1185
Bsp1407I TGTACA 1 cut(s) 271
Bsp143I GATC 6 cut(s) 84, 130, 586, 694, 746, 836
Bsp1720I GCTNAGC 3 cut(s) 96, 348, 360
Bsp19I CCATGG 1 cut(s) 963
BspACI CCGC 5 cut(s) 177, 569, 834, 999, 1012
BspANI GGCC 1 cut(s) 1185
BspCNI CTCAG 8 cut(s) 101, 109, 205, 361, 373, 382, 457, 1090
BspDI ATCGAT 1 cut(s) 608
BspFNI CGCG 2 cut(s) 179, 1014
BspLI GGNNCC 3 cut(s) 314, 417, 724
BspMAI CTGCAG 1 cut(s) 105
BspPI GGATC 4 cut(s) 138, 581, 702, 844
BspT107I GGYRCC 1 cut(s) 415
BspTI CTTAAG 1 cut(s) 125
BsrGI TGTACA 1 cut(s) 271
BsrI ACTGG 6 cut(s) 598, 794, 972, 1009, 1019, 1186
BssECI CCNNGG 3 cut(s) 778, 829, 963
BssMI GATC 6 cut(s) 84, 130, 586, 694, 746, 836
BssNAI GTATAC 1 cut(s) 735
BssT1I CCWWGG 1 cut(s) 963
Bst1107I GTATAC 1 cut(s) 735
Bst2UI CCWGG 2 cut(s) 687, 780
Bst4CI ACNGT 6 cut(s) 498, 714, 854, 873, 1084, 1151
Bst6I CTCTTC 1 cut(s) 898
BstAFI CTTAAG 1 cut(s) 125
BstAUI TGTACA 1 cut(s) 271
BstC8I GCNNGC 2 cut(s) 108, 1110
BstDEI CTNAG 8 cut(s) 88, 96, 192, 348, 360, 369, 444, 1098
BstDSI CCRYGG 2 cut(s) 829, 963
BstF5I GGATG 4 cut(s) 277, 523, 663, 887
BstFNI CGCG 2 cut(s) 179, 1014
BstHHI GCGC 1 cut(s) 685
BstKTI GATC 6 cut(s) 87, 133, 589, 697, 749, 839
BstMAI GTCTC 1 cut(s) 465
BstMBI GATC 6 cut(s) 84, 130, 586, 694, 746, 836
BstMCI CGRYCG 1 cut(s) 900
BstMWI GCNNNNNNNGC 3 cut(s) 185, 341, 883
BstNI CCWGG 2 cut(s) 687, 780
BstSCI CCNGG 3 cut(s) 308, 685, 778
BstSFI CTRYAG 3 cut(s) 101, 339, 850
BstUI CGCG 2 cut(s) 179, 1014
BstV1I GCAGC 5 cut(s) 87, 115, 356, 562, 955
BstX2I RGATCY 3 cut(s) 84, 694, 836
BstXI CCANNNNNNTGG 1 cut(s) 1175
BstYI RGATCY 3 cut(s) 84, 694, 836
BstZ17I GTATAC 1 cut(s) 735
Bsu15I ATCGAT 1 cut(s) 608
Bsu36I CCTNAGG 1 cut(s) 444
BsuRI GGCC 1 cut(s) 1185
BsuTUI ATCGAT 1 cut(s) 608
BtgI CCRYGG 2 cut(s) 829, 963
BtsCI GGATG 4 cut(s) 277, 523, 663, 887
BtsI GCAGTG 2 cut(s) 166, 1119
BtsIMutI CAGTG 6 cut(s) 166, 859, 979, 1089, 1119, 1156
Cac8I GCNNGC 2 cut(s) 108, 1110
CaiI CAGNNNCTG 1 cut(s) 995
CfoI GCGC 1 cut(s) 685
Cfr13I GGNCC 2 cut(s) 312, 1117
ClaI ATCGAT 1 cut(s) 608
CseI GACGC 1 cut(s) 168
Csp6I GTAC 2 cut(s) 272, 518
CviAII CATG 4 cut(s) 25, 727, 740, 964
CviQI GTAC 2 cut(s) 272, 518
DdeI CTNAG 8 cut(s) 88, 96, 192, 348, 360, 369, 444, 1098
DpnI GATC 6 cut(s) 86, 132, 588, 696, 748, 838
DpnII GATC 6 cut(s) 84, 130, 586, 694, 746, 836
EaeI YGGCCR 1 cut(s) 1183
Eam1104I CTCTTC 1 cut(s) 898
EarI CTCTTC 1 cut(s) 898
EciI GGCGGA 1 cut(s) 849
Eco130I CCWWGG 1 cut(s) 963
Eco47I GGWCC 2 cut(s) 312, 1117
Eco81I CCTNAGG 1 cut(s) 444
EcoRII CCWGG 2 cut(s) 685, 778
EcoT14I CCWWGG 1 cut(s) 963
EcoT22I ATGCAT 1 cut(s) 958
ErhI CCWWGG 1 cut(s) 963
FaeI CATG 4 cut(s) 28, 730, 743, 967
FaqI GGGAC 3 cut(s) 1015, 1079, 1150
FatI CATG 4 cut(s) 24, 726, 739, 963
FauI CCCGC 2 cut(s) 576, 992
FblI GTMKAC 1 cut(s) 734
Fnu4HI GCNGC 5 cut(s) 101, 104, 345, 551, 969
FokI GGATG 4 cut(s) 264, 510, 650, 874
Fsp4HI GCNGC 5 cut(s) 101, 104, 345, 551, 969
FspBI CTAG 1 cut(s) 753
GlaI GCGC 1 cut(s) 684
GluI GCNGC 5 cut(s) 101, 104, 345, 551, 969
HaeIII GGCC 1 cut(s) 1185
HapII CCGG 1 cut(s) 310
HgaI GACGC 1 cut(s) 168
HhaI GCGC 1 cut(s) 685
Hin1II CATG 4 cut(s) 28, 730, 743, 967
Hin6I GCGC 1 cut(s) 683
HinP1I GCGC 1 cut(s) 683
HincII GTYRAC 2 cut(s) 392, 560
HindII GTYRAC 2 cut(s) 392, 560
HinfI GANTC 8 cut(s) 401, 427, 556, 610, 857, 986, 992, 1056
HpaII CCGG 1 cut(s) 310
HphI GGTGA 2 cut(s) 125, 557
Hpy166II GTNNAC 5 cut(s) 34, 392, 560, 735, 1117
Hpy188I TCNGA 5 cut(s) 91, 321, 991, 1061, 1076
Hpy188III TCNNGA 4 cut(s) 13, 293, 584, 924
Hpy8I GTNNAC 5 cut(s) 34, 392, 560, 735, 1117
HpyAV CCTTC 6 cut(s) 44, 291, 296, 537, 852, 1056
HpyCH4III ACNGT 6 cut(s) 498, 714, 854, 873, 1084, 1151
HpyCH4IV ACGT 1 cut(s) 802
HpyCH4V TGCA 8 cut(s) 61, 103, 110, 166, 730, 790, 938, 956
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 341, 883
HpyF3I CTNAG 8 cut(s) 88, 96, 192, 348, 360, 369, 444, 1098
HpySE526I ACGT 1 cut(s) 802
Hsp92II CATG 4 cut(s) 28, 730, 743, 967
HspAI GCGC 1 cut(s) 683
Kzo9I GATC 6 cut(s) 84, 130, 586, 694, 746, 836
LmnI GCTCC 3 cut(s) 156, 232, 586
Lsp1109I GCAGC 5 cut(s) 87, 115, 356, 562, 955
LweI GCATC 1 cut(s) 672
MaeI CTAG 1 cut(s) 753
MaeII ACGT 1 cut(s) 802
MalI GATC 6 cut(s) 86, 132, 588, 696, 748, 838
MboI GATC 6 cut(s) 84, 130, 586, 694, 746, 836
MboII GAAGA 1 cut(s) 885
MflI RGATCY 3 cut(s) 84, 694, 836
MlsI TGGCCA 1 cut(s) 1185
MluCI AATT 1 cut(s) 757
MluNI TGGCCA 1 cut(s) 1185
MlyI GAGTC 2 cut(s) 565, 995
MmeI TCCRAC 1 cut(s) 123
Mox20I TGGCCA 1 cut(s) 1185
Mph1103I ATGCAT 1 cut(s) 958
MscI TGGCCA 1 cut(s) 1185
MseI TTAA 2 cut(s) 126, 1122
MslI CAYNNNNRTG 1 cut(s) 852
Msp20I TGGCCA 1 cut(s) 1185
MspA1I CMGCKG 1 cut(s) 100
MspCI CTTAAG 1 cut(s) 125
MspI CCGG 1 cut(s) 310
MspR9I CCNGG 3 cut(s) 310, 687, 780
MvaI CCWGG 2 cut(s) 687, 780
MvnI CGCG 2 cut(s) 179, 1014
MwoI GCNNNNNNNGC 3 cut(s) 185, 341, 883
NciI CCSGG 1 cut(s) 310
NcoI CCATGG 1 cut(s) 963
NdeII GATC 6 cut(s) 84, 130, 586, 694, 746, 836
NlaIII CATG 4 cut(s) 28, 730, 743, 967
NlaIV GGNNCC 3 cut(s) 314, 417, 724
NmeAIII GCCGAG 1 cut(s) 392
NsiI ATGCAT 1 cut(s) 958
OliI CACNNNNGTG 1 cut(s) 852
PfeI GAWTC 6 cut(s) 401, 427, 610, 857, 992, 1056
PflFI GACNNNGTC 1 cut(s) 1070
PflMI CCANNNNNTGG 1 cut(s) 142
PkrI GCNGC 5 cut(s) 102, 105, 346, 552, 970
PleI GAGTC 2 cut(s) 564, 994
PpsI GAGTC 2 cut(s) 564, 994
Psp1406I AACGTT 1 cut(s) 802
Psp6I CCWGG 2 cut(s) 685, 778
PspGI CCWGG 2 cut(s) 685, 778
PspN4I GGNNCC 3 cut(s) 314, 417, 724
PspPI GGNCC 2 cut(s) 312, 1117
PstI CTGCAG 1 cut(s) 105
PstNI CAGNNNCTG 1 cut(s) 995
PsuI RGATCY 3 cut(s) 84, 694, 836
PsyI GACNNNGTC 1 cut(s) 1070
PvuII CAGCTG 1 cut(s) 100
RsaI GTAC 2 cut(s) 273, 519
RsaNI GTAC 2 cut(s) 272, 518
RseI CAYNNNNRTG 1 cut(s) 852
SaqAI TTAA 2 cut(s) 126, 1122
SatI GCNGC 5 cut(s) 101, 104, 345, 551, 969
Sau3AI GATC 6 cut(s) 84, 130, 586, 694, 746, 836
Sau96I GGNCC 2 cut(s) 312, 1117
SchI GAGTC 2 cut(s) 565, 995
ScrFI CCNGG 3 cut(s) 310, 687, 780
SfaNI GCATC 1 cut(s) 672
SfcI CTRYAG 3 cut(s) 101, 339, 850
SinI GGWCC 2 cut(s) 312, 1117
SmiMI CAYNNNNRTG 1 cut(s) 852
SmlI CTYRAG 3 cut(s) 125, 462, 922
SmoI CTYRAG 3 cut(s) 125, 462, 922
Sse9I AATT 1 cut(s) 757
SsiI CCGC 5 cut(s) 177, 569, 834, 999, 1012
SspMI CTAG 1 cut(s) 753
StyD4I CCNGG 3 cut(s) 308, 685, 778
StyI CCWWGG 1 cut(s) 963
TaaI ACNGT 6 cut(s) 498, 714, 854, 873, 1084, 1151
TaiI ACGT 1 cut(s) 805
TaqI TCGA 1 cut(s) 608
TaqII GACCGA 2 cut(s) 759, 886
TasI AATT 1 cut(s) 757
TatI WGTACW 1 cut(s) 271
TfiI GAWTC 6 cut(s) 401, 427, 610, 857, 992, 1056
Tru1I TTAA 2 cut(s) 126, 1122
Tru9I TTAA 2 cut(s) 126, 1122
TscAI CASTG 6 cut(s) 166, 859, 979, 1089, 1119, 1156
TseI GCWGC 5 cut(s) 100, 103, 344, 550, 968
TspDTI ATGAA 4 cut(s) 13, 594, 881, 1160
TspRI CASTG 6 cut(s) 166, 859, 979, 1089, 1119, 1156
Tth111I GACNNNGTC 1 cut(s) 1070
Van91I CCANNNNNTGG 1 cut(s) 142
Vha464I CTTAAG 1 cut(s) 125
VpaK11BI GGWCC 2 cut(s) 312, 1117
XmiI GTMKAC 1 cut(s) 734
XspI CTAG 1 cut(s) 753
Zsp2I ATGCAT 1 cut(s) 958
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.