Prupe.5G168500_v2.0.a1

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0013667
Physical Location & Seq
Reverse (-)
14505335 .. 14508230
2896 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G168500.1

Sequence Viewer

Length: 1587 bp
ATGACGTCGTCGGAGTTCATGGATAAGCAGATCATGGAACTTTCTCGGTCCCACTCCGACGATTTATCTCAACTTTCATACCCTCGTGACCTCCGAGACGATGAGGACAAGGACGACGACTCAGTTTCCAGCTTTCACTTCCGCCCCACGCGACATGTCGTCTCGCAGCAGTCACGCGTTTCCACTTTCGAGAATTGCAGTTCTATGGACCACATTGACCTTGCAAAATCGGGTTGCAAGTATGCCGGGGCTTTTGATGACTCGGCATTGATCTCGGTGATTGGTCAGAAGATGAGCCATCATTTTGACAATATGCTGCATGCGGTGGAGGGTCTCAGTGCGCGACTTTCCCAACTGGAGACTCGCTTACGCCGACTAGAGAACTCAGTTGACGACTTGAAGGATTCTACTGAAATTAACCATGGCAGGAATGATGGGAAACTTAGACAACTTGAGAATATTCTAAGACAGGTTGAAGGTGGTATGCAGGATTTGAAAGATAAACAAGAGATATCAGAGGCTAAGCTGCAGCTTGCAACTCTTCAAATGTTAAAGGGTCACCAGCAATTGGAAAACCAAAGGAGCAATGTACAAACAATTTCAACTCAGGGAGTTTTGTCATCTGCCCCTCAACAAAGCCACCAACCACATTCAACTCCTGTTGCTTGTCTACAACTTTCTTCTTTTCCTCCTAATGTTCCCCCAACCCAACCCCATCAGAATTTGCCACCAGCACCTGCAACACAAGTTCATACTCACTCACCTCAGAATCAAATCCCTTCTGTTCAACAGACAGATTCTAACTACTTGCCACCTGTCTTGAATCCAAAACCCGCACCTCAGATGTACTATATGCCTCAAGTTCAGCAGTCACAACCACCACCCACAGCACCATATCAATATTATCCACCAGCACCACATCTTTCATCAACCTCACAATTACAACGGCTACCTGAATTGCACCCGCCTCTCAGTGCAGTTGATCCACAAGCCCAGTCTTCATTGGTTCATAATCCTGGAGAAATCCCCTACATGTTATCTCAAAGATATCCTCCAAGCATGCATAAACCCTCTAATGCAACTGGCATGGCTCTCCCAACCCAACAACAGTACATGGGTTCTTCCCAGTATACGCATGATCAATCCACAAGAAATCATTGTTCAGATTTATTTGCTGGGCATATGCAACCAGTTCAACATTCAACTTATGATGATTTTTATTCCCATGAAGGATCTCCATCTCACAACAGCGGTTCAAAAACGAAACACTCACAAATCTTGCCAGCCTCTTTAGACCGTTCTGGTGGAAGCAGCATTTCGAGACTGCCAACTGCCCAGGTGTTGCCATATGCAATACCTATGGCCTCGAGTATGGATAAGGAGTCAAGTTCTGGTGTGACTGGAAACAGAATACCAATTGATAATGTCATTGATGAGGTTGTAACAATGGGATTCCGAAGGGACATAGTCAGAGCAACAGTGAAGAAAATGACTGAGAATGGGCAATCAGTGGACATTAATGTTGTGCTGGATAAGCTGATGAACAATGGAGAAGTTCAACCCAAAAGTGGTGGATTTGGCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1421

Protein Analysis

529

Amino Acids

58.51

Weight (kDa)

6.13

Isoelectric Point (pI)

68.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013667)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05870 FvH4_5g05870
malus_domestica MD06G1169700.v1.1 MD14G1175200.v1.1
prunus_persica Prupe.5G168500_v2.0.a1
pyrus_communis pycom06g15100 pycom14g14620
rosa_chinensis RchiOBHm_Chr7g0192951
rosa_laevigata RLG00000004309
rosa_multiflora Rmu_sc0002040.1_g000061
rosa_roxburghii Rroxscaffold_3G00262510
rosa_rugosa Rorug07G0006400 Rorug07G0294800
rosa_samantha Rh7AG131600 Rh7BG132500 Rh7CG135700 Rh7DG133600
rosa_wichuraiana Rw7G011230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 745
AatII GACGTC 1 cut(s) 8
Acc36I ACCTGC 1 cut(s) 745
AccB7I CCANNNNNTGG 1 cut(s) 568
AccI GTMKAC 2 cut(s) 670, 1130
AccII CGCG 3 cut(s) 151, 177, 343
AciI CCGC 5 cut(s) 142, 323, 834, 965, 1251
AclWI GGATC 2 cut(s) 977, 1240
AcoI YGGCCR 1 cut(s) 1579
AcsI RAATTY 1 cut(s) 721
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 591, 848, 1112
AfiI CCNNNNNNNGG 2 cut(s) 568, 1568
AflIII ACRYGT 3 cut(s) 154, 175, 1032
AhdI GACNNNNNGTC 1 cut(s) 158
AjnI CCWGG 2 cut(s) 1015, 1335
AluBI AGCT 4 cut(s) 132, 526, 532, 1537
AluI AGCT 4 cut(s) 132, 526, 532, 1537
Alw26I GTCTC 5 cut(s) 90, 166, 338, 353, 1315
AlwI GGATC 2 cut(s) 977, 1240
AlwNI CAGNNNCTG 1 cut(s) 737
Ama87I CYCGRG 1 cut(s) 1366
AoxI GGCC 2 cut(s) 1362, 1579
ApeKI GCWGC 5 cut(s) 166, 316, 526, 529, 1311
ApoI RAATTY 1 cut(s) 721
AseI ATTAAT 1 cut(s) 1518
AspLEI GCGC 1 cut(s) 343
AspS9I GGNCC 2 cut(s) 48, 208
AsuC2I CCSGG 1 cut(s) 247
AsuHPI GGTGA 3 cut(s) 289, 551, 753
AvaI CYCGRG 1 cut(s) 1366
AvaII GGWCC 2 cut(s) 48, 208
BalI TGGCCA 1 cut(s) 1581
BauI CACGAG 1 cut(s) 84
BbsI GAAGAC 1 cut(s) 990
BbvI GCAGC 5 cut(s) 178, 303, 513, 541, 1323
BccI CCATC 4 cut(s) 306, 428, 723, 1246
BceAI ACGGC 1 cut(s) 962
BcgI CGANNNNNNTGC 2 cut(s) 255, 289
BciT130I CCWGG 2 cut(s) 1017, 1337
BclI TGATCA 1 cut(s) 1138
BcnI CCSGG 1 cut(s) 247
BcoDI GTCTC 5 cut(s) 90, 166, 338, 353, 1315
BfaI CTAG 1 cut(s) 377
BfmI CTRYAG 1 cut(s) 527
BfuAI ACCTGC 1 cut(s) 745
BisI GCNGC 5 cut(s) 167, 317, 527, 530, 1312
BlpI GCTNAGC 1 cut(s) 522
BlsI GCNGC 5 cut(s) 168, 318, 528, 531, 1313
Bme1390I CCNGG 3 cut(s) 247, 1017, 1337
Bme18I GGWCC 2 cut(s) 48, 208
BmeRI GACNNNNNGTC 1 cut(s) 158
BmeT110I CYCGRG 1 cut(s) 1366
BmgT120I GGNCC 2 cut(s) 48, 208
BmiI GGNNCC 1 cut(s) 50
BmrFI CCNGG 3 cut(s) 247, 1017, 1337
BmrI ACTGGG 2 cut(s) 988, 1120
BmuI ACTGGG 2 cut(s) 988, 1120
BpiI GAAGAC 1 cut(s) 990
BpmI CTGGAG 2 cut(s) 377, 1038
Bpu1102I GCTNAGC 1 cut(s) 522
BpuEI CTTGAG 2 cut(s) 473, 843
BpuMI CCSGG 1 cut(s) 247
BsaBI GATNNNNATC 1 cut(s) 1237
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 338
BsaJI CCNNGG 3 cut(s) 246, 421, 1335
Bsc4I CCNNNNNNNGG 2 cut(s) 568, 1568
Bse1I ACTGG 7 cut(s) 360, 994, 1087, 1126, 1190, 1405, 1582
Bse3DI GCAATG 1 cut(s) 592
Bse8I GATNNNNATC 1 cut(s) 1237
BseBI CCWGG 2 cut(s) 1017, 1337
BseDI CCNNGG 3 cut(s) 246, 421, 1335
BseJI GATNNNNATC 1 cut(s) 1237
BseLI CCNNNNNNNGG 2 cut(s) 568, 1568
BseMI GCAATG 1 cut(s) 592
BseMII CTCAG 8 cut(s) 135, 349, 399, 620, 779, 854, 985, 1485
BseNI ACTGG 7 cut(s) 360, 994, 1087, 1126, 1190, 1405, 1582
BseXI GCAGC 5 cut(s) 178, 303, 513, 541, 1323
BseYI CCCAGC 1 cut(s) 1175
BsgI GTGCAG 1 cut(s) 996
Bsh1236I CGCG 3 cut(s) 151, 177, 343
BshFI GGCC 2 cut(s) 1364, 1581
BsiHKCI CYCGRG 1 cut(s) 1366
BsiSI CCGG 1 cut(s) 246
BslFI GGGAC 2 cut(s) 34, 1475
BslI CCNNNNNNNGG 2 cut(s) 568, 1568
BsmAI GTCTC 5 cut(s) 90, 166, 338, 353, 1315
BsmBI CGTCTC 2 cut(s) 90, 166
BsmFI GGGAC 2 cut(s) 34, 1475
BsnI GGCC 2 cut(s) 1364, 1581
Bso31I GGTCTC 1 cut(s) 338
BsoBI CYCGRG 1 cut(s) 1366
Bsp1407I TGTACA 1 cut(s) 589
Bsp143I GATC 5 cut(s) 30, 270, 982, 1138, 1232
Bsp1720I GCTNAGC 1 cut(s) 522
Bsp19I CCATGG 1 cut(s) 421
BspACI CCGC 5 cut(s) 142, 323, 834, 965, 1251
BspANI GGCC 2 cut(s) 1364, 1581
BspCNI CTCAG 8 cut(s) 134, 348, 398, 619, 778, 853, 984, 1486
BspFNI CGCG 3 cut(s) 151, 177, 343
BspLI GGNNCC 1 cut(s) 50
BspMAI CTGCAG 1 cut(s) 531
BspMI ACCTGC 1 cut(s) 745
BspPI GGATC 2 cut(s) 977, 1240
BspTNI GGTCTC 1 cut(s) 338
BsrDI GCAATG 1 cut(s) 592
BsrGI TGTACA 1 cut(s) 589
BsrI ACTGG 7 cut(s) 360, 994, 1087, 1126, 1190, 1405, 1582
BssECI CCNNGG 3 cut(s) 246, 421, 1335
BssMI GATC 5 cut(s) 30, 270, 982, 1138, 1232
BssNAI GTATAC 1 cut(s) 1131
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 421
Bst1107I GTATAC 1 cut(s) 1131
Bst2BI CACGAG 1 cut(s) 84
Bst2UI CCWGG 2 cut(s) 1017, 1337
Bst4CI ACNGT 3 cut(s) 1110, 1298, 1480
Bst6I CTCTTC 1 cut(s) 546
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 589
BstC8I GCNNGC 4 cut(s) 321, 534, 1061, 1284
BstDSI CCRYGG 1 cut(s) 421
BstEII GGTNACC 1 cut(s) 557
BstFNI CGCG 3 cut(s) 151, 177, 343
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 5 cut(s) 33, 273, 985, 1141, 1235
BstMAI GTCTC 5 cut(s) 90, 166, 338, 353, 1315
BstMBI GATC 5 cut(s) 30, 270, 982, 1138, 1232
BstMWI GCNNNNNNNGC 1 cut(s) 1534
BstNI CCWGG 2 cut(s) 1017, 1337
BstNSI RCATGY 4 cut(s) 158, 323, 1036, 1063
BstPI GGTNACC 1 cut(s) 557
BstSCI CCNGG 3 cut(s) 245, 1015, 1335
BstSFI CTRYAG 1 cut(s) 527
BstUI CGCG 3 cut(s) 151, 177, 343
BstV1I GCAGC 5 cut(s) 178, 303, 513, 541, 1323
BstV2I GAAGAC 1 cut(s) 990
BstX2I RGATCY 1 cut(s) 1232
BstYI RGATCY 1 cut(s) 1232
BstZ17I GTATAC 1 cut(s) 1131
BsuRI GGCC 2 cut(s) 1364, 1581
BtgI CCRYGG 1 cut(s) 421
BtsIMutI CAGTG 4 cut(s) 343, 979, 1485, 1515
BveI ACCTGC 1 cut(s) 745
Cac8I GCNNGC 4 cut(s) 321, 534, 1061, 1284
CaiI CAGNNNCTG 1 cut(s) 737
CfoI GCGC 1 cut(s) 343
Cfr13I GGNCC 2 cut(s) 48, 208
Csp6I GTAC 3 cut(s) 590, 847, 1111
CspCI CAANNNNNGTGG 4 cut(s) 801, 836, 1552, 1587
CviQI GTAC 3 cut(s) 590, 847, 1111
DpnI GATC 5 cut(s) 32, 272, 984, 1140, 1234
DpnII GATC 5 cut(s) 30, 270, 982, 1138, 1232
DriI GACNNNNNGTC 1 cut(s) 158
EaeI YGGCCR 1 cut(s) 1579
Eam1104I CTCTTC 1 cut(s) 546
Eam1105I GACNNNNNGTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 546
EciI GGCGGA 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 421
Eco31I GGTCTC 1 cut(s) 338
Eco32I GATATC 2 cut(s) 513, 1049
Eco47I GGWCC 2 cut(s) 48, 208
Eco88I CYCGRG 1 cut(s) 1366
Eco91I GGTNACC 1 cut(s) 557
EcoO65I GGTNACC 1 cut(s) 557
EcoRII CCWGG 2 cut(s) 1015, 1335
EcoRV GATATC 2 cut(s) 513, 1049
EcoT14I CCWWGG 1 cut(s) 421
EcoT22I ATGCAT 1 cut(s) 1065
ErhI CCWWGG 1 cut(s) 421
Esp3I CGTCTC 2 cut(s) 90, 166
FaqI GGGAC 2 cut(s) 34, 1475
FauI CCCGC 2 cut(s) 841, 972
FauNDI CATATG 2 cut(s) 1182, 1348
FbaI TGATCA 1 cut(s) 1138
FblI GTMKAC 2 cut(s) 670, 1130
Fnu4HI GCNGC 5 cut(s) 167, 317, 527, 530, 1312
Fsp4HI GCNGC 5 cut(s) 167, 317, 527, 530, 1312
FspBI CTAG 1 cut(s) 377
GlaI GCGC 1 cut(s) 342
GluI GCNGC 5 cut(s) 167, 317, 527, 530, 1312
GsaI CCCAGC 1 cut(s) 1179
GsuI CTGGAG 2 cut(s) 377, 1038
HaeIII GGCC 2 cut(s) 1364, 1581
HapII CCGG 1 cut(s) 246
HhaI GCGC 1 cut(s) 343
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HinfI GANTC 9 cut(s) 119, 260, 361, 404, 769, 797, 823, 1382, 1452
HpaII CCGG 1 cut(s) 246
HphI GGTGA 3 cut(s) 289, 551, 753
Hpy166II GTNNAC 4 cut(s) 391, 671, 1131, 1513
Hpy188III TCNNGA 4 cut(s) 86, 190, 820, 1320
Hpy8I GTNNAC 4 cut(s) 391, 671, 1131, 1513
Hpy99I CGWCG 4 cut(s) 10, 13, 62, 119
HpyAV CCTTC 5 cut(s) 394, 470, 789, 1223, 1452
HpyCH4III ACNGT 3 cut(s) 1110, 1298, 1480
HpyCH4IV ACGT 1 cut(s) 5
HpyF10VI GCNNNNNNNGC 1 cut(s) 1534
HpySE526I ACGT 1 cut(s) 5
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 341
Ksp22I TGATCA 1 cut(s) 1138
Kzo9I GATC 5 cut(s) 30, 270, 982, 1138, 1232
LmnI GCTCC 1 cut(s) 582
Lsp1109I GCAGC 5 cut(s) 178, 303, 513, 541, 1323
MaeI CTAG 1 cut(s) 377
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 6 cut(s) 86, 171, 557, 870, 1396, 1441
MalI GATC 5 cut(s) 32, 272, 984, 1140, 1234
MboI GATC 5 cut(s) 30, 270, 982, 1138, 1232
MboII GAAGA 6 cut(s) 301, 533, 672, 990, 1113, 1495
MfeI CAATTG 2 cut(s) 566, 1416
MflI RGATCY 1 cut(s) 1232
MlsI TGGCCA 1 cut(s) 1581
MluCI AATT 8 cut(s) 193, 414, 566, 597, 721, 938, 956, 1416
MluI ACGCGT 1 cut(s) 175
MluNI TGGCCA 1 cut(s) 1581
MlyI GAGTC 4 cut(s) 113, 254, 355, 1391
MmeI TCCRAC 1 cut(s) 81
Mox20I TGGCCA 1 cut(s) 1581
Mph1103I ATGCAT 1 cut(s) 1065
MscI TGGCCA 1 cut(s) 1581
MseI TTAA 3 cut(s) 417, 551, 1518
Msp20I TGGCCA 1 cut(s) 1581
MspA1I CMGCKG 1 cut(s) 1251
MspI CCGG 1 cut(s) 246
MspR9I CCNGG 3 cut(s) 247, 1017, 1337
MunI CAATTG 2 cut(s) 566, 1416
MvaI CCWGG 2 cut(s) 1017, 1337
MvnI CGCG 3 cut(s) 151, 177, 343
MwoI GCNNNNNNNGC 1 cut(s) 1534
NciI CCSGG 1 cut(s) 247
NcoI CCATGG 1 cut(s) 421
NdeI CATATG 2 cut(s) 1182, 1348
NdeII GATC 5 cut(s) 30, 270, 982, 1138, 1232
NlaIV GGNNCC 1 cut(s) 50
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 5 cut(s) 86, 171, 557, 870, 1396
NsiI ATGCAT 1 cut(s) 1065
NspI RCATGY 4 cut(s) 158, 323, 1036, 1063
PaeI GCATGC 2 cut(s) 323, 1063
PaeR7I CTCGAG 1 cut(s) 1366
PaqCI CACCTGC 1 cut(s) 745
PciI ACATGT 2 cut(s) 154, 1032
PcsI WCGNNNNNNNCGW 2 cut(s) 91, 370
PfeI GAWTC 5 cut(s) 404, 769, 797, 823, 1452
PflFI GACNNNGTC 2 cut(s) 7, 1466
PflMI CCANNNNNTGG 1 cut(s) 568
PfoI TCCNGGA 1 cut(s) 1015
PkrI GCNGC 5 cut(s) 168, 318, 528, 531, 1313
PleI GAGTC 4 cut(s) 113, 254, 355, 1390
PpsI GAGTC 4 cut(s) 113, 254, 355, 1390
PscI ACATGT 2 cut(s) 154, 1032
PshBI ATTAAT 1 cut(s) 1518
Psp6I CCWGG 2 cut(s) 1015, 1335
PspEI GGTNACC 1 cut(s) 557
PspFI CCCAGC 1 cut(s) 1175
PspGI CCWGG 2 cut(s) 1015, 1335
PspN4I GGNNCC 1 cut(s) 50
PspPI GGNCC 2 cut(s) 48, 208
PspXI VCTCGAGB 1 cut(s) 1366
PstI CTGCAG 1 cut(s) 531
PstNI CAGNNNCTG 1 cut(s) 737
PsuI RGATCY 1 cut(s) 1232
PsyI GACNNNGTC 2 cut(s) 7, 1466
RsaI GTAC 3 cut(s) 591, 848, 1112
RsaNI GTAC 3 cut(s) 590, 847, 1111
SaqAI TTAA 3 cut(s) 417, 551, 1518
SatI GCNGC 5 cut(s) 167, 317, 527, 530, 1312
Sau3AI GATC 5 cut(s) 30, 270, 982, 1138, 1232
Sau96I GGNCC 2 cut(s) 48, 208
SchI GAGTC 4 cut(s) 113, 254, 355, 1391
ScrFI CCNGG 3 cut(s) 247, 1017, 1337
SfcI CTRYAG 1 cut(s) 527
Sfr274I CTCGAG 1 cut(s) 1366
SinI GGWCC 2 cut(s) 48, 208
SlaI CTCGAG 1 cut(s) 1366
SmlI CTYRAG 3 cut(s) 452, 858, 1366
SmoI CTYRAG 3 cut(s) 452, 858, 1366
SphI GCATGC 2 cut(s) 323, 1063
Sse9I AATT 8 cut(s) 193, 414, 566, 597, 721, 938, 956, 1416
SsiI CCGC 5 cut(s) 142, 323, 834, 965, 1251
SspI AATATT 2 cut(s) 460, 902
SspMI CTAG 1 cut(s) 377
StyD4I CCNGG 3 cut(s) 245, 1015, 1335
StyI CCWWGG 1 cut(s) 421
TaaI ACNGT 3 cut(s) 1110, 1298, 1480
TaiI ACGT 1 cut(s) 8
TaqI TCGA 3 cut(s) 189, 1319, 1367
TaqII GACCGA 1 cut(s) 36
TasI AATT 8 cut(s) 193, 414, 566, 597, 721, 938, 956, 1416
TatI WGTACW 3 cut(s) 589, 846, 1110
TfiI GAWTC 5 cut(s) 404, 769, 797, 823, 1452
Tru1I TTAA 3 cut(s) 417, 551, 1518
Tru9I TTAA 3 cut(s) 417, 551, 1518
TscAI CASTG 4 cut(s) 343, 979, 1485, 1515
TseFI GTSAC 5 cut(s) 86, 171, 557, 870, 1396
TseI GCWGC 5 cut(s) 166, 316, 526, 529, 1311
Tsp45I GTSAC 5 cut(s) 86, 171, 557, 870, 1396
TspDTI ATGAA 8 cut(s) 7, 66, 740, 915, 990, 998, 1242, 1556
TspRI CASTG 4 cut(s) 343, 979, 1485, 1515
Tth111I GACNNNGTC 2 cut(s) 7, 1466
Van91I CCANNNNNTGG 1 cut(s) 568
VpaK11BI GGWCC 2 cut(s) 48, 208
VspI ATTAAT 1 cut(s) 1518
XapI RAATTY 1 cut(s) 721
XceI RCATGY 4 cut(s) 158, 323, 1036, 1063
XhoI CTCGAG 1 cut(s) 1366
XmiI GTMKAC 2 cut(s) 670, 1130
XspI CTAG 1 cut(s) 377
ZraI GACGTC 1 cut(s) 6
Zsp2I ATGCAT 1 cut(s) 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.