Rw7G011230

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0013667
Physical Location & Seq
Reverse (-)
10511295 .. 10514129
2835 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G011230.1

Sequence Viewer

Length: 1572 bp
ATGAGAACAACGTCGGAGTTCATGGACAAGCAGATTATGGAACTTTCTAGATCCTACTCCGACGACTTCTCTCAGCTCACATACCCTCCTCATCCTCATCAGCTAGAAAACGACGACGACGACGACGACGACGACGACTTGCTCTCCAGCTTTCATTTCCGCCCCGTACGGCAGCAGGACTTGGCGATGATTTCGGTGATTGATGGGAAGATGAACCAGCATTTTGGCAATTTGATGCATGCAGTGGAGGGTCTCAGCGCACGAGTCTCTCAGCTGGAGACTCGGTTGCGCCTGGTAGAGAATTCTGTTGACGACTTGAAGGATTGTTCTGATGTTAACTATGGCAAGACTGATGGGAAGCTGAGGGAAGTAGAACACATTTTGAGAAAGGTTGAAGGTGGTATGCAGGATTTGAGAGATAAGCAAGAGATCTCAGAGGCTCAGCTGCAGCTTGCAAAACTTCAAATGCTTAAGGATCACCAGAAGTTGGAACAAGAAAGGAGCAATGTGCAAACAACTTCCGCGTCATCTGCTCCTCAGAAATCCCACCAATCATATTCAACTCCTGTTGCTCCTCATCAATCCCACCAATCATTTTCAACTCCTGTTGCTCCTCATCAATCCCACCAATCATATTCAACTCCTGTTGCTTCTGTACAACATCCTTCCCTTCCTCCTGATATTCCCCCAACCCTGACCCATCAGAACTTTCCACCAGCACCTACAGCAGCTCAGCTTCCAGCTCAGTCATCTCAGTATCAAACTCCTTATAGCCAACAGCCAGATTCTAACTACTCGGCACCTGTGCTGAATCCACCCACAACTCAGGCATACTATATGTCTCAAGTTCAGCAGTCGCAACCACCTCCCACAGCACCATATCAACCCTATCCAGCAGTACCACATCCTTCACAAACATCACATTTTCAGAAGCAGAGTCAGCTTCACCCGCCTCTCAATGCTCCTGATCCACAAGCCCAGTATTCATCGATTCACAATCCAGCAGAAACCCCGTATGGGTTATCTCAAAGCTATCCCCAGAGCATCCACAAAACCTCTAATGCGCCTGGCATGGTTCTACCATCACAACAACAGTTTGAGGGTTCCATGCAGTATACACATGACCGATCTACTAGAAATTACAGCACAGACTTCTCCCCTGGAAATTTGCAACCAGTTCAACGTTCAAGCTACGATGACTTTTATTCCCACGGCGGATCTCCTTCTCACTACAGTGATTCAAAAATGAAACAGTCGCAAGTATCACCATCCTCTTCGGTCGCTTCTGGTGGAAGCAGTTTCTCAAGACTGCCAACTGCAAAGGTATTGCCGTATGCATTACCCATGGCTGCCAGTGTGGATAAGGAGTCAGATTCTGGCGGGACTGGAAACCGCGTTCCAGTTGATAATGTCATTGATAAGGTTGTAGCTATGGGATTCCGAAGGGACATAGTCAGAGCAACAGTGAGGAAAATGACTGAGAATGGGCAGGCAGTGGACCTTAATGTTGTGCTGGATAAACTGATGAACAGTGGAGAAGTTCAGTCCCAAAGTGGTGGATTTGGCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1421

Protein Analysis

523

Amino Acids

57.84

Weight (kDa)

5.77

Isoelectric Point (pI)

68.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 468 - 511 4.3e-20 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013667)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05870 FvH4_5g05870
malus_domestica MD06G1169700.v1.1 MD14G1175200.v1.1
prunus_persica Prupe.5G168500_v2.0.a1
pyrus_communis pycom06g15100 pycom14g14620
rosa_chinensis RchiOBHm_Chr7g0192951
rosa_laevigata RLG00000004309
rosa_multiflora Rmu_sc0002040.1_g000061
rosa_roxburghii Rroxscaffold_3G00262510
rosa_rugosa Rorug07G0006400 Rorug07G0294800
rosa_samantha Rh7AG131600 Rh7BG132500 Rh7CG135700 Rh7DG133600
rosa_wichuraiana Rw7G011230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 799
AccB7I CCANNNNNTGG 1 cut(s) 487
AccI GTMKAC 1 cut(s) 1115
AccII CGCG 2 cut(s) 524, 1395
AciI CCGC 6 cut(s) 160, 522, 950, 1215, 1380, 1393
AclI AACGTT 1 cut(s) 1183
AclWI GGATC 4 cut(s) 45, 483, 962, 1225
AcoI YGGCCR 1 cut(s) 1564
AcsI RAATTY 2 cut(s) 301, 1165
AfaI GTAC 3 cut(s) 168, 657, 900
AfiI CCNNNNNNNGG 2 cut(s) 487, 1017
AflII CTTAAG 1 cut(s) 470
AgsI TTSAA 9 cut(s) 319, 395, 464, 561, 600, 639, 1181, 1188, 1242
AjnI CCWGG 3 cut(s) 291, 1066, 1159
AleI CACNNNNGTG 1 cut(s) 1233
AloI GAACNNNNNNTCC 2 cut(s) 1380, 1412
Alw26I GTCTC 4 cut(s) 257, 271, 272, 846
AlwI GGATC 4 cut(s) 45, 483, 962, 1225
AlwNI CAGNNNCTG 1 cut(s) 1376
AoxI GGCC 1 cut(s) 1564
ApeKI GCWGC 5 cut(s) 172, 445, 448, 728, 1349
ApoI RAATTY 2 cut(s) 301, 1165
AspLEI GCGC 3 cut(s) 260, 291, 1066
AspS9I GGNCC 1 cut(s) 1498
AsuHPI GGTGA 4 cut(s) 208, 470, 938, 1257
AvaII GGWCC 1 cut(s) 1498
BalI TGGCCA 1 cut(s) 1566
BanI GGYRCC 1 cut(s) 799
BauI CACGAG 1 cut(s) 261
BbvCI CCTCAGC 1 cut(s) 362
BbvI GCAGC 5 cut(s) 184, 432, 460, 740, 1336
BccI CCATC 5 cut(s) 197, 347, 708, 1090, 1276
BceAI ACGGC 3 cut(s) 185, 1228, 1315
BciT130I CCWGG 3 cut(s) 293, 1068, 1161
BcoDI GTCTC 4 cut(s) 257, 271, 272, 846
BfaI CTAG 3 cut(s) 48, 104, 1134
BfmI CTRYAG 3 cut(s) 446, 723, 1231
BfrI CTTAAG 1 cut(s) 470
BglII AGATCT 1 cut(s) 429
BisI GCNGC 5 cut(s) 173, 446, 449, 729, 1350
BlpI GCTNAGC 2 cut(s) 441, 732
BlsI GCNGC 5 cut(s) 174, 447, 450, 730, 1351
Bme1390I CCNGG 3 cut(s) 293, 1068, 1161
Bme18I GGWCC 1 cut(s) 1498
BmgT120I GGNCC 1 cut(s) 1498
BmiI GGNNCC 2 cut(s) 801, 1105
BmrFI CCNGG 3 cut(s) 293, 1068, 1161
BmrI ACTGGG 1 cut(s) 973
BmsI GCATC 2 cut(s) 225, 1053
BmuI ACTGGG 1 cut(s) 973
BpmI CTGGAG 2 cut(s) 130, 296
Bpu10I CCTNAGC 1 cut(s) 362
Bpu1102I GCTNAGC 2 cut(s) 441, 732
BpuEI CTTGAG 2 cut(s) 828, 1288
Bsa29I ATCGAT 1 cut(s) 989
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 3 cut(s) 1159, 1210, 1344
BsaXI ACNNNNNCTCC 6 cut(s) 70, 100, 128, 158, 269, 299
Bsc4I CCNNNNNNNGG 2 cut(s) 487, 1017
Bse1I ACTGG 6 cut(s) 979, 1175, 1353, 1390, 1400, 1567
Bse3DI GCAATG 1 cut(s) 511
BseBI CCWGG 3 cut(s) 293, 1068, 1161
BseCI ATCGAT 1 cut(s) 989
BseDI CCNNGG 3 cut(s) 1159, 1210, 1344
BseGI GGATG 5 cut(s) 91, 661, 904, 1044, 1268
BseLI CCNNNNNNNGG 2 cut(s) 487, 1017
BseMI GCAATG 1 cut(s) 511
BseNI ACTGG 6 cut(s) 979, 1175, 1353, 1390, 1400, 1567
BseRI GAGGAG 4 cut(s) 78, 525, 564, 603
BseXI GCAGC 5 cut(s) 184, 432, 460, 740, 1336
Bsh1236I CGCG 2 cut(s) 524, 1395
Bsh1285I CGRYCG 1 cut(s) 1281
BshFI GGCC 1 cut(s) 1566
BshNI GGYRCC 1 cut(s) 799
BshVI ATCGAT 1 cut(s) 989
BsiEI CGRYCG 1 cut(s) 1281
BsiWI CGTACG 1 cut(s) 166
BslFI GGGAC 3 cut(s) 1396, 1460, 1531
BslI CCNNNNNNNGG 2 cut(s) 487, 1017
BsmAI GTCTC 4 cut(s) 257, 271, 272, 846
BsmFI GGGAC 3 cut(s) 1396, 1460, 1531
BsnI GGCC 1 cut(s) 1566
Bso31I GGTCTC 1 cut(s) 257
Bsp1407I TGTACA 1 cut(s) 655
Bsp143I GATC 6 cut(s) 50, 429, 475, 967, 1127, 1217
Bsp1720I GCTNAGC 2 cut(s) 441, 732
Bsp19I CCATGG 1 cut(s) 1344
BspACI CCGC 6 cut(s) 160, 522, 950, 1215, 1380, 1393
BspANI GGCC 1 cut(s) 1566
BspDI ATCGAT 1 cut(s) 989
BspFNI CGCG 2 cut(s) 524, 1395
BspLI GGNNCC 2 cut(s) 801, 1105
BspMAI CTGCAG 1 cut(s) 450
BspPI GGATC 4 cut(s) 45, 483, 962, 1225
BspT107I GGYRCC 1 cut(s) 799
BspTI CTTAAG 1 cut(s) 470
BspTNI GGTCTC 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 511
BsrGI TGTACA 1 cut(s) 655
BsrI ACTGG 6 cut(s) 979, 1175, 1353, 1390, 1400, 1567
BssECI CCNNGG 3 cut(s) 1159, 1210, 1344
BssMI GATC 6 cut(s) 50, 429, 475, 967, 1127, 1217
BssNAI GTATAC 1 cut(s) 1116
BssSI CACGAG 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 1344
Bst1107I GTATAC 1 cut(s) 1116
Bst2BI CACGAG 1 cut(s) 261
Bst2UI CCWGG 3 cut(s) 293, 1068, 1161
Bst4CI ACNGT 5 cut(s) 1095, 1235, 1254, 1465, 1532
Bst6I CTCTTC 1 cut(s) 1279
BstAFI CTTAAG 1 cut(s) 470
BstAUI TGTACA 1 cut(s) 655
BstC8I GCNNGC 3 cut(s) 240, 453, 1491
BstDSI CCRYGG 2 cut(s) 1210, 1344
BstF5I GGATG 5 cut(s) 91, 661, 904, 1044, 1268
BstFNI CGCG 2 cut(s) 524, 1395
BstHHI GCGC 3 cut(s) 260, 291, 1066
BstKTI GATC 6 cut(s) 53, 432, 478, 970, 1130, 1220
BstMAI GTCTC 4 cut(s) 257, 271, 272, 846
BstMBI GATC 6 cut(s) 50, 429, 475, 967, 1127, 1217
BstMCI CGRYCG 1 cut(s) 1281
BstMWI GCNNNNNNNGC 4 cut(s) 530, 725, 940, 949
BstNI CCWGG 3 cut(s) 293, 1068, 1161
BstNSI RCATGY 1 cut(s) 242
BstSCI CCNGG 3 cut(s) 291, 1066, 1159
BstSFI CTRYAG 3 cut(s) 446, 723, 1231
BstUI CGCG 2 cut(s) 524, 1395
BstV1I GCAGC 5 cut(s) 184, 432, 460, 740, 1336
BstX2I RGATCY 3 cut(s) 50, 429, 1217
BstXI CCANNNNNNTGG 2 cut(s) 224, 1556
BstYI RGATCY 3 cut(s) 50, 429, 1217
BstZ17I GTATAC 1 cut(s) 1116
Bsu15I ATCGAT 1 cut(s) 989
BsuRI GGCC 1 cut(s) 1566
BsuTUI ATCGAT 1 cut(s) 989
BtgI CCRYGG 2 cut(s) 1210, 1344
BtgZI GCGATG 1 cut(s) 200
BtsCI GGATG 5 cut(s) 91, 661, 904, 1044, 1268
BtsI GCAGTG 2 cut(s) 249, 1500
BtsIMutI CAGTG 6 cut(s) 249, 1240, 1360, 1470, 1500, 1537
Cac8I GCNNGC 3 cut(s) 240, 453, 1491
CaiI CAGNNNCTG 1 cut(s) 1376
CfoI GCGC 3 cut(s) 260, 291, 1066
Cfr13I GGNCC 1 cut(s) 1498
ClaI ATCGAT 1 cut(s) 989
CseI GACGC 1 cut(s) 513
Csp6I GTAC 3 cut(s) 167, 656, 899
CviAII CATG 6 cut(s) 22, 239, 1072, 1108, 1121, 1345
CviQI GTAC 3 cut(s) 167, 656, 899
DpnI GATC 6 cut(s) 52, 431, 477, 969, 1129, 1219
DpnII GATC 6 cut(s) 50, 429, 475, 967, 1127, 1217
EaeI YGGCCR 1 cut(s) 1564
Eam1104I CTCTTC 1 cut(s) 1279
EarI CTCTTC 1 cut(s) 1279
EciI GGCGGA 2 cut(s) 149, 1230
Eco130I CCWWGG 1 cut(s) 1344
Eco31I GGTCTC 1 cut(s) 257
Eco47I GGWCC 1 cut(s) 1498
EcoRI GAATTC 1 cut(s) 301
EcoRII CCWGG 3 cut(s) 291, 1066, 1159
EcoT14I CCWWGG 1 cut(s) 1344
EcoT22I ATGCAT 2 cut(s) 240, 1339
ErhI CCWWGG 1 cut(s) 1344
FaeI CATG 6 cut(s) 25, 242, 1075, 1111, 1124, 1348
FaqI GGGAC 3 cut(s) 1396, 1460, 1531
FatI CATG 6 cut(s) 21, 238, 1071, 1107, 1120, 1344
FauI CCCGC 2 cut(s) 957, 1373
FblI GTMKAC 1 cut(s) 1115
Fnu4HI GCNGC 5 cut(s) 173, 446, 449, 729, 1350
FokI GGATG 5 cut(s) 78, 648, 891, 1031, 1255
Fsp4HI GCNGC 5 cut(s) 173, 446, 449, 729, 1350
FspBI CTAG 3 cut(s) 48, 104, 1134
GlaI GCGC 3 cut(s) 259, 290, 1065
GluI GCNGC 5 cut(s) 173, 446, 449, 729, 1350
GsuI CTGGAG 2 cut(s) 130, 296
HaeIII GGCC 1 cut(s) 1566
HgaI GACGC 1 cut(s) 513
HhaI GCGC 3 cut(s) 260, 291, 1066
Hin1II CATG 6 cut(s) 25, 242, 1075, 1111, 1124, 1348
Hin6I GCGC 3 cut(s) 258, 289, 1064
HinP1I GCGC 3 cut(s) 258, 289, 1064
HincII GTYRAC 2 cut(s) 310, 337
HindII GTYRAC 2 cut(s) 310, 337
HpaI GTTAAC 1 cut(s) 337
HphI GGTGA 4 cut(s) 208, 470, 938, 1257
Hpy166II GTNNAC 4 cut(s) 310, 337, 1116, 1498
Hpy188III TCNNGA 4 cut(s) 48, 677, 965, 1305
Hpy8I GTNNAC 4 cut(s) 310, 337, 1116, 1498
HpyAV CCTTC 7 cut(s) 313, 389, 675, 680, 918, 1233, 1437
HpyCH4III ACNGT 5 cut(s) 1095, 1235, 1254, 1465, 1532
HpyCH4IV ACGT 2 cut(s) 11, 1183
HpyF10VI GCNNNNNNNGC 4 cut(s) 530, 725, 940, 949
HpySE526I ACGT 2 cut(s) 11, 1183
Hsp92II CATG 6 cut(s) 25, 242, 1075, 1111, 1124, 1348
HspAI GCGC 3 cut(s) 258, 289, 1064
KspAI GTTAAC 1 cut(s) 337
Kzo9I GATC 6 cut(s) 50, 429, 475, 967, 1127, 1217
LmnI GCTCC 5 cut(s) 501, 538, 577, 616, 967
Lsp1109I GCAGC 5 cut(s) 184, 432, 460, 740, 1336
LweI GCATC 2 cut(s) 225, 1053
MaeI CTAG 3 cut(s) 48, 104, 1134
MaeII ACGT 2 cut(s) 11, 1183
MalI GATC 6 cut(s) 52, 431, 477, 969, 1129, 1219
MboI GATC 6 cut(s) 50, 429, 475, 967, 1127, 1217
MboII GAAGA 2 cut(s) 220, 1266
MflI RGATCY 3 cut(s) 50, 429, 1217
MlsI TGGCCA 1 cut(s) 1566
MluCI AATT 4 cut(s) 229, 301, 1138, 1165
MluNI TGGCCA 1 cut(s) 1566
MlyI GAGTC 4 cut(s) 273, 274, 946, 1376
MmeI TCCRAC 2 cut(s) 84, 468
Mox20I TGGCCA 1 cut(s) 1566
Mph1103I ATGCAT 2 cut(s) 240, 1339
MscI TGGCCA 1 cut(s) 1566
MseI TTAA 3 cut(s) 336, 471, 1503
MslI CAYNNNNRTG 1 cut(s) 1233
Msp20I TGGCCA 1 cut(s) 1566
MspA1I CMGCKG 2 cut(s) 274, 445
MspCI CTTAAG 1 cut(s) 470
MspR9I CCNGG 3 cut(s) 293, 1068, 1161
MvaI CCWGG 3 cut(s) 293, 1068, 1161
MvnI CGCG 2 cut(s) 524, 1395
MwoI GCNNNNNNNGC 4 cut(s) 530, 725, 940, 949
NcoI CCATGG 1 cut(s) 1344
NdeII GATC 6 cut(s) 50, 429, 475, 967, 1127, 1217
NlaIII CATG 6 cut(s) 25, 242, 1075, 1111, 1124, 1348
NlaIV GGNNCC 2 cut(s) 801, 1105
NmeAIII GCCGAG 1 cut(s) 776
NsiI ATGCAT 2 cut(s) 240, 1339
NspI RCATGY 1 cut(s) 242
OliI CACNNNNGTG 1 cut(s) 1233
PaeI GCATGC 1 cut(s) 242
PcsI WCGNNNNNNNCGW 6 cut(s) 117, 120, 123, 126, 129, 132
PfeI GAWTC 6 cut(s) 785, 811, 991, 1238, 1373, 1437
Pfl23II CGTACG 1 cut(s) 166
PflFI GACNNNGTC 1 cut(s) 1451
PflMI CCANNNNNTGG 1 cut(s) 487
PkrI GCNGC 5 cut(s) 174, 447, 450, 730, 1351
PleI GAGTC 4 cut(s) 272, 274, 945, 1375
PpsI GAGTC 4 cut(s) 272, 274, 945, 1375
Psp1406I AACGTT 1 cut(s) 1183
Psp6I CCWGG 3 cut(s) 291, 1066, 1159
PspGI CCWGG 3 cut(s) 291, 1066, 1159
PspLI CGTACG 1 cut(s) 166
PspN4I GGNNCC 2 cut(s) 801, 1105
PspPI GGNCC 1 cut(s) 1498
PstI CTGCAG 1 cut(s) 450
PstNI CAGNNNCTG 1 cut(s) 1376
PsuI RGATCY 3 cut(s) 50, 429, 1217
PsyI GACNNNGTC 1 cut(s) 1451
PvuII CAGCTG 2 cut(s) 274, 445
RsaI GTAC 3 cut(s) 168, 657, 900
RsaNI GTAC 3 cut(s) 167, 656, 899
RseI CAYNNNNRTG 1 cut(s) 1233
SaqAI TTAA 3 cut(s) 336, 471, 1503
SatI GCNGC 5 cut(s) 173, 446, 449, 729, 1350
Sau3AI GATC 6 cut(s) 50, 429, 475, 967, 1127, 1217
Sau96I GGNCC 1 cut(s) 1498
SchI GAGTC 4 cut(s) 273, 274, 946, 1376
ScrFI CCNGG 3 cut(s) 293, 1068, 1161
SfaNI GCATC 2 cut(s) 225, 1053
SfcI CTRYAG 3 cut(s) 446, 723, 1231
SinI GGWCC 1 cut(s) 1498
SmiMI CAYNNNNRTG 1 cut(s) 1233
SmlI CTYRAG 3 cut(s) 470, 843, 1303
SmoI CTYRAG 3 cut(s) 470, 843, 1303
SphI GCATGC 1 cut(s) 242
Sse9I AATT 4 cut(s) 229, 301, 1138, 1165
SsiI CCGC 6 cut(s) 160, 522, 950, 1215, 1380, 1393
SspMI CTAG 3 cut(s) 48, 104, 1134
StyD4I CCNGG 3 cut(s) 291, 1066, 1159
StyI CCWWGG 1 cut(s) 1344
TaaI ACNGT 5 cut(s) 1095, 1235, 1254, 1465, 1532
TaiI ACGT 2 cut(s) 14, 1186
TaqI TCGA 1 cut(s) 989
TaqII GACCGA 2 cut(s) 1140, 1267
TasI AATT 4 cut(s) 229, 301, 1138, 1165
TatI WGTACW 1 cut(s) 655
TfiI GAWTC 6 cut(s) 785, 811, 991, 1238, 1373, 1437
Tru1I TTAA 3 cut(s) 336, 471, 1503
Tru9I TTAA 3 cut(s) 336, 471, 1503
TscAI CASTG 6 cut(s) 249, 1240, 1360, 1470, 1500, 1537
TseI GCWGC 5 cut(s) 172, 445, 448, 728, 1349
TspDTI ATGAA 6 cut(s) 10, 143, 227, 975, 1262, 1541
TspRI CASTG 6 cut(s) 249, 1240, 1360, 1470, 1500, 1537
Tth111I GACNNNGTC 1 cut(s) 1451
Van91I CCANNNNNTGG 1 cut(s) 487
Vha464I CTTAAG 1 cut(s) 470
VpaK11BI GGWCC 1 cut(s) 1498
XapI RAATTY 2 cut(s) 301, 1165
XbaI TCTAGA 1 cut(s) 47
XceI RCATGY 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 1115
XspI CTAG 3 cut(s) 48, 104, 1134
Zsp2I ATGCAT 2 cut(s) 240, 1339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.