Rh7BG132500

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0013667
Physical Location & Seq
Reverse (-)
9858756 .. 9861724
2969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG132500.1

Sequence Viewer

Length: 1422 bp
ATGTTGTGTTGTGACTCACAGAATTGTGGTTCTATGGATCAAACTGAACGTGCGAAATCCAGGGACTTGGCGATGATTTCGGTGATTGATGGGAAGATGAACCAGCATTTTGGCAATTTGATGCATGCAGTGGAGGGTCTCAGCGCACGAGTCTCTCAGCTGGAGACTCGGTTGCGCCTGGTAGAGAATTCTGTTGACGACTTGAAGGATTGTTCTGATGTTAACTATGGCAAGACTGATGGGAAGCTGAGGGAAGTAGAACACATTTTGAGAAAGGTTGAAGGTGGTATGCAGGATTTGAGAGATAAGCAAGAGATCTCAGAGGCTCAGCTGCAGCTTGCAAAACTTCAAATGCTTAAGGATCACCAGAAGTTGGAACAAGAAAGGAGCAATGTCCAAACAACTTCCGCGTCATCTGCTCCTCAGCAATCCCACCAATCATATTCAACTCCTGTTGCTCCTCATCAATCCCACCAATCATATTCAACTCCTGTTGCTTCTGTACAACATCCTTCCCTTCCTCCTGATATTCCCCGAACCCTGACCCATCAGAACTTTCCACCAGCACCTACAGCAGCTCAGCTTCCAGCTCAGTCATCTCAGTATCAAACTCCTTATAGTCAACAGCCAGATTCTAACTACTCGGCACCTGTGTTGAATCCACCACCCACACCTCAGGCATACTATATGTCTCAAGTTCAGCAGTCGCAACCACCTCCCACAGCACCATATCAACCCTATCCAGCAGTACCACATCCTTCACAAACATCACATTTTCAGCAGCAGAGTCAACTTCACCCGCCTCTCAATGCTCCTGATCCACAAGCCCAGTATTCATCGATTCACAATCCAGCAGAAACCCCGTATGGGTTATGTCAAAGCTATCCCCAGAGCATCCACAAAACCTCTAATGCGCCTGGCGTGGTTCTACCATCACAACAACAGTTTGAGGGTTCCATGCAGTATACACATGACCGATCTACTAGAAATTACAGCACAGACTTCTCCCCTGGAAATATGCAACCAGTTCAACGTTCAAGCTACGATGACTTTTATTCCCACGGCGGATCTCCTTCTCACTACAGTGATTCAAAAATGAAACAGTCGCAAGTATCGCCATCCTCTTCGGTCGCTTCTGGTGGAAGCAGTTTCTCAAGACTGCCAACTGCAAAGGTATTGCCGTATGCATTACCCATGGCTGCCAGTGTGGATAAGGAGTCAGATTCTGGCGGGACTGGAAACCGCGTTCCAGTTGATAATGTCATTGATAAGGTTGTAGCTATGGGATTCCGAAGGGACATAGTCAGAGCAACAGTGAGGAAAATGACTGAGAATGGGCAGGCAGTGGACCTTAATGTTGTGCTGGATAAACTGATGAACAGTGGAGAAGTTCAGTCCCAAAGTGGTGGATTTGGCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1421

Protein Analysis

473

Amino Acids

51.86

Weight (kDa)

6.27

Isoelectric Point (pI)

69.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 418 - 461 3.8e-20 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013667)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05870 FvH4_5g05870
malus_domestica MD06G1169700.v1.1 MD14G1175200.v1.1
prunus_persica Prupe.5G168500_v2.0.a1
pyrus_communis pycom06g15100 pycom14g14620
rosa_chinensis RchiOBHm_Chr7g0192951
rosa_laevigata RLG00000004309
rosa_multiflora Rmu_sc0002040.1_g000061
rosa_roxburghii Rroxscaffold_3G00262510
rosa_rugosa Rorug07G0006400 Rorug07G0294800
rosa_samantha Rh7AG131600 Rh7BG132500 Rh7CG135700 Rh7DG133600
rosa_wichuraiana Rw7G011230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 646
AccB7I CCANNNNNTGG 1 cut(s) 373
AccI GTMKAC 1 cut(s) 965
AccII CGCG 2 cut(s) 410, 1245
AciI CCGC 5 cut(s) 408, 800, 1065, 1230, 1243
AclI AACGTT 1 cut(s) 1033
AclWI GGATC 4 cut(s) 45, 369, 812, 1075
AcoI YGGCCR 1 cut(s) 1414
AcsI RAATTY 1 cut(s) 187
AfaI GTAC 2 cut(s) 504, 750
AfiI CCNNNNNNNGG 2 cut(s) 373, 867
AflII CTTAAG 1 cut(s) 356
AgsI TTSAA 9 cut(s) 205, 281, 350, 447, 486, 658, 1031, 1038, 1092
AjnI CCWGG 4 cut(s) 59, 177, 916, 1009
AleI CACNNNNGTG 1 cut(s) 1083
AloI GAACNNNNNNTCC 2 cut(s) 1230, 1262
Alw26I GTCTC 4 cut(s) 143, 157, 158, 696
AlwI GGATC 4 cut(s) 45, 369, 812, 1075
AlwNI CAGNNNCTG 1 cut(s) 1226
AoxI GGCC 1 cut(s) 1414
ApeKI GCWGC 5 cut(s) 331, 334, 575, 781, 1199
ApoI RAATTY 1 cut(s) 187
AspLEI GCGC 3 cut(s) 146, 177, 916
AspS9I GGNCC 1 cut(s) 1348
AsuHPI GGTGA 3 cut(s) 94, 356, 788
AvaII GGWCC 1 cut(s) 1348
AxyI CCTNAGG 1 cut(s) 675
BalI TGGCCA 1 cut(s) 1416
BanI GGYRCC 1 cut(s) 646
BauI CACGAG 1 cut(s) 147
BbvCI CCTCAGC 2 cut(s) 248, 423
BbvI GCAGC 5 cut(s) 318, 346, 587, 793, 1186
BccI CCATC 5 cut(s) 83, 233, 555, 940, 1126
BceAI ACGGC 2 cut(s) 1078, 1165
BciT130I CCWGG 4 cut(s) 61, 179, 918, 1011
BcoDI GTCTC 4 cut(s) 143, 157, 158, 696
BfaI CTAG 1 cut(s) 984
BfmI CTRYAG 3 cut(s) 332, 570, 1081
BfrI CTTAAG 1 cut(s) 356
BglII AGATCT 1 cut(s) 315
BisI GCNGC 5 cut(s) 332, 335, 576, 782, 1200
BlpI GCTNAGC 2 cut(s) 327, 579
BlsI GCNGC 5 cut(s) 333, 336, 577, 783, 1201
Bme1390I CCNGG 4 cut(s) 61, 179, 918, 1011
Bme18I GGWCC 1 cut(s) 1348
BmgT120I GGNCC 1 cut(s) 1348
BmiI GGNNCC 2 cut(s) 648, 955
BmrFI CCNGG 4 cut(s) 61, 179, 918, 1011
BmrI ACTGGG 1 cut(s) 823
BmsI GCATC 2 cut(s) 111, 903
BmuI ACTGGG 1 cut(s) 823
BpmI CTGGAG 1 cut(s) 182
Bpu10I CCTNAGC 2 cut(s) 248, 423
Bpu1102I GCTNAGC 2 cut(s) 327, 579
BpuEI CTTGAG 2 cut(s) 678, 1138
Bsa29I ATCGAT 1 cut(s) 839
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 4 cut(s) 60, 1009, 1060, 1194
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 2 cut(s) 373, 867
Bse1I ACTGG 6 cut(s) 829, 1025, 1203, 1240, 1250, 1417
Bse21I CCTNAGG 1 cut(s) 675
Bse3DI GCAATG 1 cut(s) 397
BseBI CCWGG 4 cut(s) 61, 179, 918, 1011
BseCI ATCGAT 1 cut(s) 839
BseDI CCNNGG 4 cut(s) 60, 1009, 1060, 1194
BseGI GGATG 4 cut(s) 508, 754, 894, 1118
BseLI CCNNNNNNNGG 2 cut(s) 373, 867
BseMI GCAATG 1 cut(s) 397
BseNI ACTGG 6 cut(s) 829, 1025, 1203, 1240, 1250, 1417
BseRI GAGGAG 2 cut(s) 411, 450
BseXI GCAGC 5 cut(s) 318, 346, 587, 793, 1186
Bsh1236I CGCG 2 cut(s) 410, 1245
Bsh1285I CGRYCG 1 cut(s) 1131
BshFI GGCC 1 cut(s) 1416
BshNI GGYRCC 1 cut(s) 646
BshVI ATCGAT 1 cut(s) 839
BsiEI CGRYCG 1 cut(s) 1131
BslFI GGGAC 4 cut(s) 77, 1246, 1310, 1381
BslI CCNNNNNNNGG 2 cut(s) 373, 867
BsmAI GTCTC 4 cut(s) 143, 157, 158, 696
BsmFI GGGAC 4 cut(s) 77, 1246, 1310, 1381
BsnI GGCC 1 cut(s) 1416
Bso31I GGTCTC 1 cut(s) 143
Bsp1407I TGTACA 1 cut(s) 502
Bsp143I GATC 6 cut(s) 37, 315, 361, 817, 977, 1067
Bsp1720I GCTNAGC 2 cut(s) 327, 579
Bsp19I CCATGG 1 cut(s) 1194
BspACI CCGC 5 cut(s) 408, 800, 1065, 1230, 1243
BspANI GGCC 1 cut(s) 1416
BspDI ATCGAT 1 cut(s) 839
BspFNI CGCG 2 cut(s) 410, 1245
BspLI GGNNCC 2 cut(s) 648, 955
BspMAI CTGCAG 1 cut(s) 336
BspPI GGATC 4 cut(s) 45, 369, 812, 1075
BspT107I GGYRCC 1 cut(s) 646
BspTI CTTAAG 1 cut(s) 356
BspTNI GGTCTC 1 cut(s) 143
BsrDI GCAATG 1 cut(s) 397
BsrGI TGTACA 1 cut(s) 502
BsrI ACTGG 6 cut(s) 829, 1025, 1203, 1240, 1250, 1417
BssECI CCNNGG 4 cut(s) 60, 1009, 1060, 1194
BssMI GATC 6 cut(s) 37, 315, 361, 817, 977, 1067
BssNAI GTATAC 1 cut(s) 966
BssSI CACGAG 1 cut(s) 147
BssT1I CCWWGG 1 cut(s) 1194
Bst1107I GTATAC 1 cut(s) 966
Bst2BI CACGAG 1 cut(s) 147
Bst2UI CCWGG 4 cut(s) 61, 179, 918, 1011
Bst4CI ACNGT 5 cut(s) 945, 1085, 1104, 1315, 1382
Bst6I CTCTTC 1 cut(s) 1129
BstAFI CTTAAG 1 cut(s) 356
BstAUI TGTACA 1 cut(s) 502
BstC8I GCNNGC 3 cut(s) 126, 339, 1341
BstDSI CCRYGG 2 cut(s) 1060, 1194
BstF5I GGATG 4 cut(s) 508, 754, 894, 1118
BstFNI CGCG 2 cut(s) 410, 1245
BstHHI GCGC 3 cut(s) 146, 177, 916
BstKTI GATC 6 cut(s) 40, 318, 364, 820, 980, 1070
BstMAI GTCTC 4 cut(s) 143, 157, 158, 696
BstMBI GATC 6 cut(s) 37, 315, 361, 817, 977, 1067
BstMCI CGRYCG 1 cut(s) 1131
BstMWI GCNNNNNNNGC 3 cut(s) 416, 572, 1114
BstNI CCWGG 4 cut(s) 61, 179, 918, 1011
BstNSI RCATGY 1 cut(s) 128
BstSCI CCNGG 4 cut(s) 59, 177, 916, 1009
BstSFI CTRYAG 3 cut(s) 332, 570, 1081
BstUI CGCG 2 cut(s) 410, 1245
BstV1I GCAGC 5 cut(s) 318, 346, 587, 793, 1186
BstX2I RGATCY 2 cut(s) 315, 1067
BstXI CCANNNNNNTGG 3 cut(s) 67, 110, 1406
BstYI RGATCY 2 cut(s) 315, 1067
BstZ17I GTATAC 1 cut(s) 966
Bsu15I ATCGAT 1 cut(s) 839
Bsu36I CCTNAGG 1 cut(s) 675
BsuRI GGCC 1 cut(s) 1416
BsuTUI ATCGAT 1 cut(s) 839
BtgI CCRYGG 2 cut(s) 1060, 1194
BtgZI GCGATG 1 cut(s) 86
BtsCI GGATG 4 cut(s) 508, 754, 894, 1118
BtsI GCAGTG 2 cut(s) 135, 1350
BtsIMutI CAGTG 6 cut(s) 135, 1090, 1210, 1320, 1350, 1387
Cac8I GCNNGC 3 cut(s) 126, 339, 1341
CaiI CAGNNNCTG 1 cut(s) 1226
CfoI GCGC 3 cut(s) 146, 177, 916
Cfr13I GGNCC 1 cut(s) 1348
ClaI ATCGAT 1 cut(s) 839
CseI GACGC 1 cut(s) 399
Csp6I GTAC 2 cut(s) 503, 749
CviAII CATG 4 cut(s) 125, 958, 971, 1195
CviQI GTAC 2 cut(s) 503, 749
DpnI GATC 6 cut(s) 39, 317, 363, 819, 979, 1069
DpnII GATC 6 cut(s) 37, 315, 361, 817, 977, 1067
EaeI YGGCCR 1 cut(s) 1414
Eam1104I CTCTTC 1 cut(s) 1129
EarI CTCTTC 1 cut(s) 1129
EciI GGCGGA 1 cut(s) 1080
Eco130I CCWWGG 1 cut(s) 1194
Eco31I GGTCTC 1 cut(s) 143
Eco47I GGWCC 1 cut(s) 1348
Eco81I CCTNAGG 1 cut(s) 675
EcoRI GAATTC 1 cut(s) 187
EcoRII CCWGG 4 cut(s) 59, 177, 916, 1009
EcoT14I CCWWGG 1 cut(s) 1194
EcoT22I ATGCAT 2 cut(s) 126, 1189
ErhI CCWWGG 1 cut(s) 1194
FaeI CATG 4 cut(s) 128, 961, 974, 1198
FaqI GGGAC 4 cut(s) 77, 1246, 1310, 1381
FatI CATG 4 cut(s) 124, 957, 970, 1194
FauI CCCGC 2 cut(s) 807, 1223
FblI GTMKAC 1 cut(s) 965
Fnu4HI GCNGC 5 cut(s) 332, 335, 576, 782, 1200
FokI GGATG 4 cut(s) 495, 741, 881, 1105
Fsp4HI GCNGC 5 cut(s) 332, 335, 576, 782, 1200
FspBI CTAG 1 cut(s) 984
GlaI GCGC 3 cut(s) 145, 176, 915
GluI GCNGC 5 cut(s) 332, 335, 576, 782, 1200
GsuI CTGGAG 1 cut(s) 182
HaeIII GGCC 1 cut(s) 1416
HgaI GACGC 1 cut(s) 399
HhaI GCGC 3 cut(s) 146, 177, 916
Hin1II CATG 4 cut(s) 128, 961, 974, 1198
Hin6I GCGC 3 cut(s) 144, 175, 914
HinP1I GCGC 3 cut(s) 144, 175, 914
HincII GTYRAC 4 cut(s) 196, 223, 623, 791
HindII GTYRAC 4 cut(s) 196, 223, 623, 791
HpaI GTTAAC 1 cut(s) 223
HphI GGTGA 3 cut(s) 94, 356, 788
Hpy166II GTNNAC 6 cut(s) 196, 223, 623, 791, 966, 1348
Hpy188I TCNGA 6 cut(s) 217, 322, 552, 1222, 1292, 1307
Hpy188III TCNNGA 3 cut(s) 524, 815, 1155
Hpy8I GTNNAC 6 cut(s) 196, 223, 623, 791, 966, 1348
HpyAV CCTTC 7 cut(s) 199, 275, 522, 527, 768, 1083, 1287
HpyCH4III ACNGT 5 cut(s) 945, 1085, 1104, 1315, 1382
HpyCH4IV ACGT 2 cut(s) 49, 1033
HpyCH4V TGCA 9 cut(s) 124, 128, 292, 334, 341, 961, 1021, 1169, 1187
HpyF10VI GCNNNNNNNGC 3 cut(s) 416, 572, 1114
HpySE526I ACGT 2 cut(s) 49, 1033
Hsp92II CATG 4 cut(s) 128, 961, 974, 1198
HspAI GCGC 3 cut(s) 144, 175, 914
KspAI GTTAAC 1 cut(s) 223
Kzo9I GATC 6 cut(s) 37, 315, 361, 817, 977, 1067
LmnI GCTCC 4 cut(s) 387, 424, 463, 817
Lsp1109I GCAGC 5 cut(s) 318, 346, 587, 793, 1186
LweI GCATC 2 cut(s) 111, 903
MaeI CTAG 1 cut(s) 984
MaeII ACGT 2 cut(s) 49, 1033
MaeIII GTNAC 1 cut(s) 11
MalI GATC 6 cut(s) 39, 317, 363, 819, 979, 1069
MboI GATC 6 cut(s) 37, 315, 361, 817, 977, 1067
MboII GAAGA 2 cut(s) 106, 1116
MflI RGATCY 2 cut(s) 315, 1067
MlsI TGGCCA 1 cut(s) 1416
MluCI AATT 4 cut(s) 22, 115, 187, 988
MluNI TGGCCA 1 cut(s) 1416
MlyI GAGTC 5 cut(s) 8, 159, 160, 796, 1226
MmeI TCCRAC 1 cut(s) 354
Mox20I TGGCCA 1 cut(s) 1416
Mph1103I ATGCAT 2 cut(s) 126, 1189
MscI TGGCCA 1 cut(s) 1416
MseI TTAA 3 cut(s) 222, 357, 1353
MslI CAYNNNNRTG 1 cut(s) 1083
Msp20I TGGCCA 1 cut(s) 1416
MspA1I CMGCKG 2 cut(s) 160, 331
MspCI CTTAAG 1 cut(s) 356
MspR9I CCNGG 4 cut(s) 61, 179, 918, 1011
MvaI CCWGG 4 cut(s) 61, 179, 918, 1011
MvnI CGCG 2 cut(s) 410, 1245
MwoI GCNNNNNNNGC 3 cut(s) 416, 572, 1114
NcoI CCATGG 1 cut(s) 1194
NdeII GATC 6 cut(s) 37, 315, 361, 817, 977, 1067
NlaIII CATG 4 cut(s) 128, 961, 974, 1198
NlaIV GGNNCC 2 cut(s) 648, 955
NmeAIII GCCGAG 1 cut(s) 623
NmuCI GTSAC 1 cut(s) 11
NsiI ATGCAT 2 cut(s) 126, 1189
NspI RCATGY 1 cut(s) 128
OliI CACNNNNGTG 1 cut(s) 1083
PaeI GCATGC 1 cut(s) 128
PfeI GAWTC 6 cut(s) 632, 658, 841, 1088, 1223, 1287
PflFI GACNNNGTC 1 cut(s) 1301
PflMI CCANNNNNTGG 1 cut(s) 373
PkrI GCNGC 5 cut(s) 333, 336, 577, 783, 1201
PleI GAGTC 5 cut(s) 8, 158, 160, 795, 1225
PpsI GAGTC 5 cut(s) 8, 158, 160, 795, 1225
Psp1406I AACGTT 1 cut(s) 1033
Psp6I CCWGG 4 cut(s) 59, 177, 916, 1009
PspGI CCWGG 4 cut(s) 59, 177, 916, 1009
PspN4I GGNNCC 2 cut(s) 648, 955
PspPI GGNCC 1 cut(s) 1348
PstI CTGCAG 1 cut(s) 336
PstNI CAGNNNCTG 1 cut(s) 1226
PsuI RGATCY 2 cut(s) 315, 1067
PsyI GACNNNGTC 1 cut(s) 1301
PvuII CAGCTG 2 cut(s) 160, 331
RsaI GTAC 2 cut(s) 504, 750
RsaNI GTAC 2 cut(s) 503, 749
RseI CAYNNNNRTG 1 cut(s) 1083
SaqAI TTAA 3 cut(s) 222, 357, 1353
SatI GCNGC 5 cut(s) 332, 335, 576, 782, 1200
Sau3AI GATC 6 cut(s) 37, 315, 361, 817, 977, 1067
Sau96I GGNCC 1 cut(s) 1348
SchI GAGTC 5 cut(s) 8, 159, 160, 796, 1226
ScrFI CCNGG 4 cut(s) 61, 179, 918, 1011
SfaNI GCATC 2 cut(s) 111, 903
SfcI CTRYAG 3 cut(s) 332, 570, 1081
SinI GGWCC 1 cut(s) 1348
SmiMI CAYNNNNRTG 1 cut(s) 1083
SmlI CTYRAG 3 cut(s) 356, 693, 1153
SmoI CTYRAG 3 cut(s) 356, 693, 1153
SphI GCATGC 1 cut(s) 128
Sse9I AATT 4 cut(s) 22, 115, 187, 988
SsiI CCGC 5 cut(s) 408, 800, 1065, 1230, 1243
SspMI CTAG 1 cut(s) 984
StyD4I CCNGG 4 cut(s) 59, 177, 916, 1009
StyI CCWWGG 1 cut(s) 1194
TaaI ACNGT 5 cut(s) 945, 1085, 1104, 1315, 1382
TaiI ACGT 2 cut(s) 52, 1036
TaqI TCGA 1 cut(s) 839
TaqII GACCGA 2 cut(s) 990, 1117
TasI AATT 4 cut(s) 22, 115, 187, 988
TatI WGTACW 1 cut(s) 502
TfiI GAWTC 6 cut(s) 632, 658, 841, 1088, 1223, 1287
Tru1I TTAA 3 cut(s) 222, 357, 1353
Tru9I TTAA 3 cut(s) 222, 357, 1353
TscAI CASTG 6 cut(s) 135, 1090, 1210, 1320, 1350, 1387
TseFI GTSAC 1 cut(s) 11
TseI GCWGC 5 cut(s) 331, 334, 575, 781, 1199
Tsp45I GTSAC 1 cut(s) 11
TspDTI ATGAA 4 cut(s) 113, 825, 1112, 1391
TspRI CASTG 6 cut(s) 135, 1090, 1210, 1320, 1350, 1387
Tth111I GACNNNGTC 1 cut(s) 1301
Van91I CCANNNNNTGG 1 cut(s) 373
Vha464I CTTAAG 1 cut(s) 356
VpaK11BI GGWCC 1 cut(s) 1348
XapI RAATTY 1 cut(s) 187
XceI RCATGY 1 cut(s) 128
XmiI GTMKAC 1 cut(s) 965
XspI CTAG 1 cut(s) 984
Zsp2I ATGCAT 2 cut(s) 126, 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.