Rorug07G0294800

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0013667
Physical Location & Seq
Forward (+)
28884561 .. 28895117
10557 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0294800.1

Sequence Viewer

Length: 1224 bp
ATGGAGCTTTGGACGCTCTTCGTCACCGCATCAGTTCCGGTCTTGGAATCGTTCTTGATTACTGCGCTTGGCTCATATCTTGCACTTGATCGCGTGAACCTTTTGGGCCCAGATTGCAGGAAGTACTTGAACACTATTGTATTCTATGTGAACACTCCAGCCCTGATTGCTGCCAACCTTGCAGAGACAATAACATATGACAGCCTGGTTAAACTGTGGTTCATGCCGGTGAATATTCTTCTTACATTCATTGTTGGTTCAATATTTGGATGGATACTCATGAAATTAACAAGAACTCCTCCACATTTGCGAGGTCTCGTCTTGGGTTGTTGCGCTGCTGGAAATTTGGGAAACCTTCTCCTCATTATTATTCCAGCAGTTTGTGAAGAATCAGGGAGTCCATTTGGAGATGCTGATACCTGCGATACTACTGGGACTACTTATGCTTCACTTTCAATGGCGATTGGAGCCATTTATTTGTGGACCTATGTGTATAATATTGTGCGGATATCTTCAAAGACGAGCATCCAAGATTCCGATTCCCCGCAGACCACCGACAGCTCGTCAACATCCAGCCACATAAGTTACACCGAGCCTCTGCTTTCTATAGAGGACAATGTGGATCAATGTGAACCTCCCCGTAATGTATCTGAAGGAGATGCTAAGATGACGTGTCTAGGTAAAACAAAGGAATGGATGGTGATGGTGAATGAAAAGCTCAATTTGAAAATAGTATTTTCCCCCTCAACTATTGCAGTGATTATTGGGTTTGCAGTTGGAATCATATCTCCAATTCGAAACTTATTGATTGGAGACGACGCTCCTCTAGCGGTGATCGACGACACTACTTCGTTGTTAGGAGATGCTGCTATCCCGCTTCTTACTTTGATAATAGGAGGTAACCTTGTTAAAGGTTTGAGAGTGGGAGGGATACAAAAATCTATCCTTATTGGCATCATAATTGTTAAATATGTTGCGGCGCCTCTTACAGGAATATTGATTGTTAAAGGGGCAGTAAAATTTGGCTTTGTAAGCAATGATCCATTGTATCTGTTTGTTCTTCTGCTTCAGTTTGCTGTCCCTCCTGCAATGAACTTGGGAACCATCACTCAACTGTTCGGACAAGGAGAGAGTGAGTGTTCAGTTATCATGCTCTGGACCTACATTTTTGCTTCGCTATCGCTTACGTTTTGGTGTGCCTTCTTCTTGTGGCTTGTGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1421

Protein Analysis

407

Amino Acids

44.01

Weight (kDa)

4.77

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 10 - 398 1.2e-78 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013667)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05870 FvH4_5g05870
malus_domestica MD06G1169700.v1.1 MD14G1175200.v1.1
prunus_persica Prupe.5G168500_v2.0.a1
pyrus_communis pycom06g15100 pycom14g14620
rosa_chinensis RchiOBHm_Chr7g0192951
rosa_laevigata RLG00000004309
rosa_multiflora Rmu_sc0002040.1_g000061
rosa_roxburghii Rroxscaffold_3G00262510
rosa_rugosa Rorug07G0006400 Rorug07G0294800
rosa_samantha Rh7AG131600 Rh7BG132500 Rh7CG135700 Rh7DG133600
rosa_wichuraiana Rw7G011230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 428
AccB1I GGYRCC 1 cut(s) 979
AccII CGCG 1 cut(s) 93
AciI CCGC 6 cut(s) 27, 505, 545, 830, 875, 977
AclWI GGATC 2 cut(s) 630, 1034
AcsI RAATTY 2 cut(s) 343, 1019
AcuI CTGAAG 2 cut(s) 672, 1052
AcyI GRCGYC 1 cut(s) 980
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 1 cut(s) 989
AflIII ACRYGT 1 cut(s) 671
AgsI TTSAA 5 cut(s) 130, 261, 456, 516, 727
AhdI GACNNNNNGTC 1 cut(s) 562
AjiI CACGTC 1 cut(s) 672
AjnI CCWGG 1 cut(s) 204
AluBI AGCT 3 cut(s) 7, 561, 718
AluI AGCT 3 cut(s) 7, 561, 718
Alw26I GTCTC 3 cut(s) 179, 320, 807
AlwI GGATC 2 cut(s) 630, 1034
AoxI GGCC 2 cut(s) 106, 1218
ApaI GGGCCC 1 cut(s) 110
ApeKI GCWGC 3 cut(s) 170, 335, 866
ApoI RAATTY 2 cut(s) 343, 1019
AspLEI GCGC 3 cut(s) 67, 335, 982
AspS9I GGNCC 4 cut(s) 106, 107, 483, 1158
AsuHPI GGTGA 5 cut(s) 16, 241, 712, 718, 844
AsuII TTCGAA 1 cut(s) 796
AvaII GGWCC 2 cut(s) 483, 1158
BaeGI GKGCMC 1 cut(s) 110
BanI GGYRCC 1 cut(s) 979
BanII GRGCYC 1 cut(s) 110
BbvI GCAGC 3 cut(s) 157, 322, 853
BccI CCATC 4 cut(s) 264, 691, 697, 1112
BciT130I CCWGG 1 cut(s) 206
BciVI GTATCC 2 cut(s) 267, 924
BcoDI GTCTC 3 cut(s) 179, 320, 807
BfaI CTAG 2 cut(s) 677, 827
BfmI CTRYAG 1 cut(s) 606
BfoI RGCGCY 1 cut(s) 983
BfuAI ACCTGC 1 cut(s) 428
BfuI GTATCC 2 cut(s) 267, 924
BisI GCNGC 4 cut(s) 171, 336, 867, 978
BlsI GCNGC 4 cut(s) 172, 337, 868, 979
BmcAI AGTACT 1 cut(s) 125
Bme1390I CCNGG 1 cut(s) 206
Bme18I GGWCC 2 cut(s) 483, 1158
BmeRI GACNNNNNGTC 1 cut(s) 562
BmgBI CACGTC 1 cut(s) 672
BmgT120I GGNCC 4 cut(s) 106, 107, 483, 1158
BmiI GGNNCC 4 cut(s) 108, 469, 981, 1102
BmrFI CCNGG 1 cut(s) 206
BmrI ACTGGG 1 cut(s) 441
BmsI GCATC 6 cut(s) 38, 400, 534, 649, 853, 963
BmuI ACTGGG 1 cut(s) 441
BpmI CTGGAG 1 cut(s) 141
Bpu14I TTCGAA 1 cut(s) 796
BsaHI GRCGYC 1 cut(s) 980
BsaI GGTCTC 1 cut(s) 320
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bsc4I CCNNNNNNNGG 1 cut(s) 989
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 1 cut(s) 436
Bse3DI GCAATG 2 cut(s) 1042, 1095
BseBI CCWGG 1 cut(s) 206
BseGI GGATG 4 cut(s) 275, 525, 569, 702
BseLI CCNNNNNNNGG 1 cut(s) 989
BseMI GCAATG 2 cut(s) 1042, 1095
BseNI ACTGG 1 cut(s) 436
BseRI GAGGAG 3 cut(s) 288, 350, 813
BseSI GKGCMC 1 cut(s) 110
BseXI GCAGC 3 cut(s) 157, 322, 853
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 2 cut(s) 108, 1220
BshNI GGYRCC 1 cut(s) 979
BsiSI CCGG 2 cut(s) 38, 227
BslFI GGGAC 2 cut(s) 448, 1064
BslI CCNNNNNNNGG 1 cut(s) 989
BsmAI GTCTC 3 cut(s) 179, 320, 807
BsmBI CGTCTC 1 cut(s) 807
BsmFI GGGAC 2 cut(s) 448, 1064
BsnI GGCC 2 cut(s) 108, 1220
Bso31I GGTCTC 1 cut(s) 320
Bsp119I TTCGAA 1 cut(s) 796
Bsp120I GGGCCC 1 cut(s) 106
Bsp1286I GDGCHC 1 cut(s) 110
Bsp143I GATC 4 cut(s) 88, 622, 834, 1039
BspACI CCGC 6 cut(s) 27, 505, 545, 830, 875, 977
BspANI GGCC 2 cut(s) 108, 1220
BspFNI CGCG 1 cut(s) 93
BspHI TCATGA 1 cut(s) 279
BspLI GGNNCC 4 cut(s) 108, 469, 981, 1102
BspMI ACCTGC 1 cut(s) 428
BspPI GGATC 2 cut(s) 630, 1034
BspQI GCTCTTC 1 cut(s) 23
BspT104I TTCGAA 1 cut(s) 796
BspT107I GGYRCC 1 cut(s) 979
BspTNI GGTCTC 1 cut(s) 320
BsrDI GCAATG 2 cut(s) 1042, 1095
BsrFI RCCGGY 1 cut(s) 226
BsrI ACTGG 1 cut(s) 436
BssAI RCCGGY 1 cut(s) 226
BssMI GATC 4 cut(s) 88, 622, 834, 1039
BssNI GRCGYC 1 cut(s) 980
Bst2UI CCWGG 1 cut(s) 206
Bst4CI ACNGT 2 cut(s) 216, 1116
Bst6I CTCTTC 1 cut(s) 23
BstACI GRCGYC 1 cut(s) 980
BstBI TTCGAA 1 cut(s) 796
BstDEI CTNAG 1 cut(s) 663
BstEII GGTNACC 1 cut(s) 899
BstENI CCTNNNNNAGG 1 cut(s) 987
BstF5I GGATG 4 cut(s) 275, 525, 569, 702
BstFNI CGCG 1 cut(s) 93
BstH2I RGCGCY 1 cut(s) 983
BstHHI GCGC 3 cut(s) 67, 335, 982
BstKTI GATC 4 cut(s) 91, 625, 837, 1042
BstMAI GTCTC 3 cut(s) 179, 320, 807
BstMBI GATC 4 cut(s) 88, 622, 834, 1039
BstMWI GCNNNNNNNGC 7 cut(s) 13, 114, 167, 179, 467, 827, 1032
BstNI CCWGG 1 cut(s) 206
BstPI GGTNACC 1 cut(s) 899
BstSCI CCNGG 1 cut(s) 204
BstSFI CTRYAG 1 cut(s) 606
BstSLI GKGCMC 1 cut(s) 110
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 3 cut(s) 157, 322, 853
BsuI GTATCC 2 cut(s) 267, 924
BsuRI GGCC 2 cut(s) 108, 1220
BtrI CACGTC 1 cut(s) 672
BtsCI GGATG 4 cut(s) 275, 525, 569, 702
BtsI GCAGTG 1 cut(s) 762
BtsIMutI CAGTG 1 cut(s) 762
BveI ACCTGC 1 cut(s) 428
CciI TCATGA 1 cut(s) 279
CfoI GCGC 3 cut(s) 67, 335, 982
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 4 cut(s) 106, 107, 483, 1158
CseI GACGC 2 cut(s) 22, 827
Csp6I GTAC 1 cut(s) 124
CviAII CATG 3 cut(s) 223, 280, 1150
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 1 cut(s) 663
DinI GGCGCC 1 cut(s) 981
DpnI GATC 4 cut(s) 90, 624, 836, 1041
DpnII GATC 4 cut(s) 88, 622, 834, 1039
DriI GACNNNNNGTC 1 cut(s) 562
Eam1104I CTCTTC 1 cut(s) 23
Eam1105I GACNNNNNGTC 1 cut(s) 562
EarI CTCTTC 1 cut(s) 23
Eco24I GRGCYC 1 cut(s) 110
Eco31I GGTCTC 1 cut(s) 320
Eco32I GATATC 1 cut(s) 510
Eco47I GGWCC 2 cut(s) 483, 1158
Eco57I CTGAAG 2 cut(s) 672, 1052
Eco91I GGTNACC 1 cut(s) 899
EcoNI CCTNNNNNAGG 1 cut(s) 987
EcoO65I GGTNACC 1 cut(s) 899
EcoRII CCWGG 1 cut(s) 204
EcoRV GATATC 1 cut(s) 510
EcoT38I GRGCYC 1 cut(s) 110
EgeI GGCGCC 1 cut(s) 981
EheI GGCGCC 1 cut(s) 981
Esp3I CGTCTC 1 cut(s) 807
FaeI CATG 3 cut(s) 226, 283, 1153
FaqI GGGAC 2 cut(s) 448, 1064
FatI CATG 3 cut(s) 222, 279, 1149
FauI CCCGC 2 cut(s) 552, 882
FauNDI CATATG 1 cut(s) 196
Fnu4HI GCNGC 4 cut(s) 171, 336, 867, 978
FokI GGATG 4 cut(s) 282, 512, 556, 709
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 4 cut(s) 171, 336, 867, 978
FspBI CTAG 2 cut(s) 677, 827
GlaI GCGC 3 cut(s) 66, 334, 981
GluI GCNGC 4 cut(s) 171, 336, 867, 978
GsuI CTGGAG 1 cut(s) 141
HaeII RGCGCY 1 cut(s) 983
HaeIII GGCC 2 cut(s) 108, 1220
HapII CCGG 2 cut(s) 38, 227
HgaI GACGC 2 cut(s) 22, 827
HhaI GCGC 3 cut(s) 67, 335, 982
Hin1I GRCGYC 1 cut(s) 980
Hin1II CATG 3 cut(s) 226, 283, 1153
Hin6I GCGC 3 cut(s) 65, 333, 980
HinP1I GCGC 3 cut(s) 65, 333, 980
HincII GTYRAC 1 cut(s) 567
HindII GTYRAC 1 cut(s) 567
HinfI GANTC 6 cut(s) 47, 389, 397, 533, 539, 780
HpaII CCGG 2 cut(s) 38, 227
HphI GGTGA 5 cut(s) 16, 241, 712, 718, 844
Hpy166II GTNNAC 5 cut(s) 97, 151, 483, 567, 632
Hpy188I TCNGA 3 cut(s) 538, 652, 1121
Hpy188III TCNNGA 3 cut(s) 55, 280, 1156
Hpy8I GTNNAC 5 cut(s) 97, 151, 483, 567, 632
Hpy99I CGWCG 2 cut(s) 821, 842
HpyAV CCTTC 3 cut(s) 365, 647, 1210
HpyCH4III ACNGT 2 cut(s) 216, 1116
HpyCH4IV ACGT 2 cut(s) 671, 1187
HpyCH4V TGCA 6 cut(s) 83, 117, 182, 755, 773, 1088
HpyF10VI GCNNNNNNNGC 7 cut(s) 13, 114, 167, 179, 467, 827, 1032
HpyF3I CTNAG 1 cut(s) 663
HpySE526I ACGT 2 cut(s) 671, 1187
Hsp92I GRCGYC 1 cut(s) 980
Hsp92II CATG 3 cut(s) 226, 283, 1153
HspAI GCGC 3 cut(s) 65, 333, 980
KasI GGCGCC 1 cut(s) 979
Kzo9I GATC 4 cut(s) 88, 622, 834, 1039
LguI GCTCTTC 1 cut(s) 23
LmnI GCTCC 3 cut(s) 4, 467, 826
Lsp1109I GCAGC 3 cut(s) 157, 322, 853
LweI GCATC 6 cut(s) 38, 400, 534, 649, 853, 963
MaeI CTAG 2 cut(s) 677, 827
MaeII ACGT 2 cut(s) 671, 1187
MaeIII GTNAC 3 cut(s) 22, 584, 899
MalI GATC 4 cut(s) 90, 624, 836, 1041
MboI GATC 4 cut(s) 88, 622, 834, 1039
MboII GAAGA 6 cut(s) 10, 230, 398, 504, 1052, 1195
MhlI GDGCHC 1 cut(s) 110
MluCI AATT 6 cut(s) 284, 343, 721, 792, 960, 1019
Mly113I GGCGCC 1 cut(s) 980
MlyI GAGTC 1 cut(s) 406
MmeI TCCRAC 1 cut(s) 757
MseI TTAA 5 cut(s) 210, 287, 909, 966, 1005
MslI CAYNNNNRTG 1 cut(s) 227
MspI CCGG 2 cut(s) 38, 227
MspR9I CCNGG 1 cut(s) 206
MvaI CCWGG 1 cut(s) 206
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 7 cut(s) 13, 114, 167, 179, 467, 827, 1032
NarI GGCGCC 1 cut(s) 980
NdeI CATATG 1 cut(s) 196
NdeII GATC 4 cut(s) 88, 622, 834, 1039
NlaIII CATG 3 cut(s) 226, 283, 1153
NlaIV GGNNCC 4 cut(s) 108, 469, 981, 1102
NmuCI GTSAC 1 cut(s) 22
NspV TTCGAA 1 cut(s) 796
PagI TCATGA 1 cut(s) 279
PciSI GCTCTTC 1 cut(s) 23
PfeI GAWTC 5 cut(s) 47, 389, 533, 539, 780
PkrI GCNGC 4 cut(s) 172, 337, 868, 979
PleI GAGTC 1 cut(s) 405
PluTI GGCGCC 1 cut(s) 983
PpsI GAGTC 1 cut(s) 405
Psp6I CCWGG 1 cut(s) 204
PspEI GGTNACC 1 cut(s) 899
PspGI CCWGG 1 cut(s) 204
PspN4I GGNNCC 4 cut(s) 108, 469, 981, 1102
PspOMI GGGCCC 1 cut(s) 106
PspPI GGNCC 4 cut(s) 106, 107, 483, 1158
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
RseI CAYNNNNRTG 1 cut(s) 227
SapI GCTCTTC 1 cut(s) 23
SaqAI TTAA 5 cut(s) 210, 287, 909, 966, 1005
SatI GCNGC 4 cut(s) 171, 336, 867, 978
Sau3AI GATC 4 cut(s) 88, 622, 834, 1039
Sau96I GGNCC 4 cut(s) 106, 107, 483, 1158
ScaI AGTACT 1 cut(s) 125
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 1 cut(s) 206
SduI GDGCHC 1 cut(s) 110
SfaNI GCATC 6 cut(s) 38, 400, 534, 649, 853, 963
SfcI CTRYAG 1 cut(s) 606
SfoI GGCGCC 1 cut(s) 981
SfuI TTCGAA 1 cut(s) 796
SinI GGWCC 2 cut(s) 483, 1158
SmiMI CAYNNNNRTG 1 cut(s) 227
Sse9I AATT 6 cut(s) 284, 343, 721, 792, 960, 1019
SsiI CCGC 6 cut(s) 27, 505, 545, 830, 875, 977
SspDI GGCGCC 1 cut(s) 979
SspI AATATT 4 cut(s) 235, 264, 499, 996
SspMI CTAG 2 cut(s) 677, 827
StyD4I CCNGG 1 cut(s) 204
TaaI ACNGT 2 cut(s) 216, 1116
TaiI ACGT 2 cut(s) 674, 1190
TaqI TCGA 2 cut(s) 796, 837
TasI AATT 6 cut(s) 284, 343, 721, 792, 960, 1019
TatI WGTACW 1 cut(s) 123
TauI GCSGC 1 cut(s) 980
TfiI GAWTC 5 cut(s) 47, 389, 533, 539, 780
Tru1I TTAA 5 cut(s) 210, 287, 909, 966, 1005
Tru9I TTAA 5 cut(s) 210, 287, 909, 966, 1005
TscAI CASTG 1 cut(s) 762
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 3 cut(s) 170, 335, 866
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 5 cut(s) 211, 238, 296, 726, 1106
TspRI CASTG 1 cut(s) 762
VpaK11BI GGWCC 2 cut(s) 483, 1158
XagI CCTNNNNNAGG 1 cut(s) 987
XapI RAATTY 2 cut(s) 343, 1019
XspI CTAG 2 cut(s) 677, 827
ZrmI AGTACT 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.