Rroxscaffold_3G00262510

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013667
Physical Location & Seq
Forward (+)
56528084 .. 56530771
2688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00262510.1

Sequence Viewer

Length: 1701 bp
ATGAGGCTGGGGCTTCAACTTGTAAGTCAATTATCGTTGACACCTCGATCTCCTTGTTTGCACTCAAAAGACAAATCTGAGCTTGGAATCTCAGAAAAGCATGACGCCAAATTACGCAATAAGCCCAACAATCCTACGCCGATCACGATGAGAACAACGCCGGAGTTCATGGACAAGCAGATTATGGAACTTTCTAGATCCTACTCCGACGACTTCTCTCAGCTCACACACCCTCCTCATCCTCATCCTCATCAACTAGAAAACGACGACGACGACGACTTGCTCTCCAGCTTTCATTTCCGCCCCGTACGGCATCAGTCACGCGCCTCCACGCTCGAGAATCGTGGTTCTATGGATCAAACTGAACGTGCAAAATCCAGGGACTTGGCGATGATTTCGGTGATTGATGGGAAGATGAACCAGCATTTTGGCAATTTGATGCATGCAGTGGAGGGTCTCAGCGCACGAGTCTCTCAGCTGGAGACTCGGTTGCGCCTGGTAGAGAATTCTGTTGACGACTTGAAGGATTGTTCTGATGTTAACTATGGCAAGACTGATGGGAAGCTGAGGGAAGTAGAACACATTTTGAGAAAGGTTGAAGGTGGTATGCAGGATTTGAGAGATAAGCAAGAGATCTCAGAGGCTCAGCTGCAGCTTGCAAAACTTCAAATGCTTAAGGATCACCAGAAGTTGGAACAAGAAAGGAGCAATGTGCAAACAACTTCCGCGTCATCTGCTCCTCAGCAATCCCACCAATCATATTCAACTCCTGTTGCTCCTCATCAATCCCACCAATCATATTCAACTCCTGTTGCTCCTCATCAATCCCACCAATCATATTCAACTCCTGTTGCTTCTGTACAACATCCTTCCCTTCCTCCTGATATTCCCCCAACCCTGACCCATCAGAACTTTCCACCAGCACCTACAGCAGCTCAGCTTCCAGCTCAGTCATCTCAGTATCAAACTCCTTATAGTCAACAGCCAGATTCTAACTACTCGGCACCTGTGTTGAATCCACCACCCACACCTCAGGCATACTATATGTCTCAAGTTCAGCAGTCGCAACCACCTCCCACAGCACCATATCAACCCTATCCAGCAGTACCACATCCTTCACAAACATCACATTTTCAGCAGCAGAGTCAACTTCACCTGCCTCTCAATGCTCCTGATCCACAAGCCCAGTATTCATCGATTCACAATCCAGCAGAAACCCCGTATGGGTTATCTCAAAGCTATCCCCAGAGCATCCACAACACCTCTAATGCGCCTGGCGTGGTTCTACCATCACAACAACAGTTTGAGGGTTCCATGCAGTATACACATGACCGATCTACTAGAAATTACAGCACAGACTTCTCCCCTGGAAATATGCAACCATCGCAAGTATCGCCATCCTCTTTGGTCGCTTCTGGTGGAAGCAGTTTCTCAAGACTGCCAACTGCAAAGGTATTGCCGTATGCATTACCCATGGCTGCCAGTGTGGATAAGGAGTCAGATTCTGGCGGGACTGGAAACCGCGTTCCAGTTGATAATGTCATTGATAAGGTTGTAGCTATGGGATTCCGAAGGGACATAGTCAGAGCAACAGTGAGGAAAATGACTGAGAATGGGCAGGCAGTGGACCTTAATGTTGTGCTGGATAAACTGATGAACAGTGGAGAAGTTCAGTCCCAAAGTGGTGGATTTGGCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1421

Protein Analysis

566

Amino Acids

62.55

Weight (kDa)

6.33

Isoelectric Point (pI)

62.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 511 - 554 4.8e-20 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013667)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05870 FvH4_5g05870
malus_domestica MD06G1169700.v1.1 MD14G1175200.v1.1
prunus_persica Prupe.5G168500_v2.0.a1
pyrus_communis pycom06g15100 pycom14g14620
rosa_chinensis RchiOBHm_Chr7g0192951
rosa_laevigata RLG00000004309
rosa_multiflora Rmu_sc0002040.1_g000061
rosa_roxburghii Rroxscaffold_3G00262510
rosa_rugosa Rorug07G0006400 Rorug07G0294800
rosa_samantha Rh7AG131600 Rh7BG132500 Rh7CG135700 Rh7DG133600
rosa_wichuraiana Rw7G011230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1164
Acc36I ACCTGC 1 cut(s) 1164
AccB1I GGYRCC 1 cut(s) 1003
AccB7I CCANNNNNTGG 1 cut(s) 691
AccI GTMKAC 1 cut(s) 1322
AccII CGCG 3 cut(s) 324, 728, 1524
AciI CCGC 4 cut(s) 301, 726, 1509, 1522
AclWI GGATC 4 cut(s) 192, 363, 687, 1169
AcoI YGGCCR 1 cut(s) 1693
AcsI RAATTY 1 cut(s) 505
AcyI GRCGYC 1 cut(s) 105
AfaI GTAC 3 cut(s) 309, 861, 1107
AfiI CCNNNNNNNGG 2 cut(s) 691, 1224
AflII CTTAAG 1 cut(s) 674
AgsI TTSAA 8 cut(s) 17, 523, 599, 668, 765, 804, 843, 1015
AjnI CCWGG 4 cut(s) 377, 495, 1273, 1366
AloI GAACNNNNNNTCC 2 cut(s) 1509, 1541
Alw26I GTCTC 4 cut(s) 461, 475, 476, 1053
AlwI GGATC 4 cut(s) 192, 363, 687, 1169
AlwNI CAGNNNCTG 1 cut(s) 1505
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 1 cut(s) 1693
ApeKI GCWGC 5 cut(s) 649, 652, 932, 1138, 1478
ApoI RAATTY 1 cut(s) 505
AspLEI GCGC 4 cut(s) 326, 464, 495, 1273
AspS9I GGNCC 1 cut(s) 1627
AsuHPI GGTGA 3 cut(s) 412, 674, 1145
AvaI CYCGRG 1 cut(s) 335
AvaII GGWCC 1 cut(s) 1627
AxyI CCTNAGG 1 cut(s) 1032
BalI TGGCCA 1 cut(s) 1695
BanI GGYRCC 1 cut(s) 1003
BauI CACGAG 1 cut(s) 465
BbvCI CCTCAGC 2 cut(s) 566, 741
BbvI GCAGC 5 cut(s) 636, 664, 944, 1150, 1465
BccI CCATC 6 cut(s) 401, 551, 912, 1297, 1390, 1405
BceAI ACGGC 2 cut(s) 326, 1444
BciT130I CCWGG 4 cut(s) 379, 497, 1275, 1368
BcoDI GTCTC 4 cut(s) 461, 475, 476, 1053
BfaI CTAG 3 cut(s) 195, 257, 1341
BfmI CTRYAG 2 cut(s) 650, 927
BfrI CTTAAG 1 cut(s) 674
BfuAI ACCTGC 1 cut(s) 1164
BglII AGATCT 1 cut(s) 633
BisI GCNGC 5 cut(s) 650, 653, 933, 1139, 1479
BlpI GCTNAGC 2 cut(s) 645, 936
BlsI GCNGC 5 cut(s) 651, 654, 934, 1140, 1480
Bme1390I CCNGG 4 cut(s) 379, 497, 1275, 1368
Bme18I GGWCC 1 cut(s) 1627
BmeT110I CYCGRG 1 cut(s) 335
BmgT120I GGNCC 1 cut(s) 1627
BmiI GGNNCC 2 cut(s) 1005, 1312
BmrFI CCNGG 4 cut(s) 379, 497, 1275, 1368
BmrI ACTGGG 1 cut(s) 1180
BmsI GCATC 3 cut(s) 322, 429, 1260
BmuI ACTGGG 1 cut(s) 1180
BpmI CTGGAG 2 cut(s) 271, 500
Bpu10I CCTNAGC 2 cut(s) 566, 741
Bpu1102I GCTNAGC 2 cut(s) 645, 936
BpuEI CTTGAG 2 cut(s) 1035, 1417
Bsa29I ATCGAT 1 cut(s) 1196
BsaHI GRCGYC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 3 cut(s) 378, 1366, 1473
BsaXI ACNNNNNCTCC 6 cut(s) 217, 247, 269, 299, 473, 503
Bsc4I CCNNNNNNNGG 2 cut(s) 691, 1224
Bse1I ACTGG 5 cut(s) 1186, 1482, 1519, 1529, 1696
Bse21I CCTNAGG 1 cut(s) 1032
Bse3DI GCAATG 1 cut(s) 715
BseBI CCWGG 4 cut(s) 379, 497, 1275, 1368
BseCI ATCGAT 1 cut(s) 1196
BseDI CCNNGG 3 cut(s) 378, 1366, 1473
BseGI GGATG 6 cut(s) 238, 244, 865, 1111, 1251, 1397
BseLI CCNNNNNNNGG 2 cut(s) 691, 1224
BseMI GCAATG 1 cut(s) 715
BseNI ACTGG 5 cut(s) 1186, 1482, 1519, 1529, 1696
BseRI GAGGAG 4 cut(s) 225, 729, 768, 807
BseXI GCAGC 5 cut(s) 636, 664, 944, 1150, 1465
BseYI CCCAGC 1 cut(s) 7
Bsh1236I CGCG 3 cut(s) 324, 728, 1524
BshFI GGCC 1 cut(s) 1695
BshNI GGYRCC 1 cut(s) 1003
BshVI ATCGAT 1 cut(s) 1196
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 1 cut(s) 161
BsiWI CGTACG 1 cut(s) 307
BslFI GGGAC 4 cut(s) 395, 1525, 1589, 1660
BslI CCNNNNNNNGG 2 cut(s) 691, 1224
BsmAI GTCTC 4 cut(s) 461, 475, 476, 1053
BsmFI GGGAC 4 cut(s) 395, 1525, 1589, 1660
BsnI GGCC 1 cut(s) 1695
Bso31I GGTCTC 1 cut(s) 461
BsoBI CYCGRG 1 cut(s) 335
Bsp1407I TGTACA 1 cut(s) 859
Bsp143I GATC 8 cut(s) 47, 141, 197, 355, 633, 679, 1174, 1334
Bsp1720I GCTNAGC 2 cut(s) 645, 936
Bsp19I CCATGG 1 cut(s) 1473
BspACI CCGC 4 cut(s) 301, 726, 1509, 1522
BspANI GGCC 1 cut(s) 1695
BspDI ATCGAT 1 cut(s) 1196
BspFNI CGCG 3 cut(s) 324, 728, 1524
BspLI GGNNCC 2 cut(s) 1005, 1312
BspMAI CTGCAG 1 cut(s) 654
BspMI ACCTGC 1 cut(s) 1164
BspPI GGATC 4 cut(s) 192, 363, 687, 1169
BspT107I GGYRCC 1 cut(s) 1003
BspTI CTTAAG 1 cut(s) 674
BspTNI GGTCTC 1 cut(s) 461
BsrDI GCAATG 1 cut(s) 715
BsrGI TGTACA 1 cut(s) 859
BsrI ACTGG 5 cut(s) 1186, 1482, 1519, 1529, 1696
BssECI CCNNGG 3 cut(s) 378, 1366, 1473
BssMI GATC 8 cut(s) 47, 141, 197, 355, 633, 679, 1174, 1334
BssNAI GTATAC 1 cut(s) 1323
BssNI GRCGYC 1 cut(s) 105
BssSI CACGAG 1 cut(s) 465
BssT1I CCWWGG 1 cut(s) 1473
Bst1107I GTATAC 1 cut(s) 1323
Bst2BI CACGAG 1 cut(s) 465
Bst2UI CCWGG 4 cut(s) 379, 497, 1275, 1368
Bst4CI ACNGT 3 cut(s) 1302, 1594, 1661
BstACI GRCGYC 1 cut(s) 105
BstAFI CTTAAG 1 cut(s) 674
BstAUI TGTACA 1 cut(s) 859
BstC8I GCNNGC 3 cut(s) 444, 657, 1620
BstDSI CCRYGG 1 cut(s) 1473
BstF5I GGATG 6 cut(s) 238, 244, 865, 1111, 1251, 1397
BstFNI CGCG 3 cut(s) 324, 728, 1524
BstHHI GCGC 4 cut(s) 326, 464, 495, 1273
BstKTI GATC 8 cut(s) 50, 144, 200, 358, 636, 682, 1177, 1337
BstMAI GTCTC 4 cut(s) 461, 475, 476, 1053
BstMBI GATC 8 cut(s) 47, 141, 197, 355, 633, 679, 1174, 1334
BstMWI GCNNNNNNNGC 4 cut(s) 734, 929, 1384, 1393
BstNI CCWGG 4 cut(s) 379, 497, 1275, 1368
BstNSI RCATGY 1 cut(s) 446
BstSCI CCNGG 4 cut(s) 377, 495, 1273, 1366
BstSFI CTRYAG 2 cut(s) 650, 927
BstUI CGCG 3 cut(s) 324, 728, 1524
BstV1I GCAGC 5 cut(s) 636, 664, 944, 1150, 1465
BstX2I RGATCY 2 cut(s) 197, 633
BstXI CCANNNNNNTGG 3 cut(s) 385, 428, 1685
BstYI RGATCY 2 cut(s) 197, 633
BstZ17I GTATAC 1 cut(s) 1323
Bsu15I ATCGAT 1 cut(s) 1196
Bsu36I CCTNAGG 1 cut(s) 1032
BsuRI GGCC 1 cut(s) 1695
BsuTUI ATCGAT 1 cut(s) 1196
BtgI CCRYGG 1 cut(s) 1473
BtgZI GCGATG 2 cut(s) 404, 1368
BtsCI GGATG 6 cut(s) 238, 244, 865, 1111, 1251, 1397
BtsI GCAGTG 2 cut(s) 453, 1629
BtsIMutI CAGTG 5 cut(s) 453, 1489, 1599, 1629, 1666
BveI ACCTGC 1 cut(s) 1164
Cac8I GCNNGC 3 cut(s) 444, 657, 1620
CaiI CAGNNNCTG 1 cut(s) 1505
CfoI GCGC 4 cut(s) 326, 464, 495, 1273
Cfr13I GGNCC 1 cut(s) 1627
ClaI ATCGAT 1 cut(s) 1196
CseI GACGC 2 cut(s) 113, 717
Csp6I GTAC 3 cut(s) 308, 860, 1106
CviAII CATG 6 cut(s) 101, 169, 443, 1315, 1328, 1474
CviQI GTAC 3 cut(s) 308, 860, 1106
DpnI GATC 8 cut(s) 49, 143, 199, 357, 635, 681, 1176, 1336
DpnII GATC 8 cut(s) 47, 141, 197, 355, 633, 679, 1174, 1334
EaeI YGGCCR 1 cut(s) 1693
EciI GGCGGA 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 1473
Eco31I GGTCTC 1 cut(s) 461
Eco47I GGWCC 1 cut(s) 1627
Eco81I CCTNAGG 1 cut(s) 1032
Eco88I CYCGRG 1 cut(s) 335
EcoRI GAATTC 1 cut(s) 505
EcoRII CCWGG 4 cut(s) 377, 495, 1273, 1366
EcoT14I CCWWGG 1 cut(s) 1473
EcoT22I ATGCAT 2 cut(s) 444, 1468
ErhI CCWWGG 1 cut(s) 1473
FaeI CATG 6 cut(s) 104, 172, 446, 1318, 1331, 1477
FaqI GGGAC 4 cut(s) 395, 1525, 1589, 1660
FatI CATG 6 cut(s) 100, 168, 442, 1314, 1327, 1473
FauI CCCGC 1 cut(s) 1502
FblI GTMKAC 1 cut(s) 1322
Fnu4HI GCNGC 5 cut(s) 650, 653, 933, 1139, 1479
FokI GGATG 6 cut(s) 225, 231, 852, 1098, 1238, 1384
Fsp4HI GCNGC 5 cut(s) 650, 653, 933, 1139, 1479
FspBI CTAG 3 cut(s) 195, 257, 1341
GlaI GCGC 4 cut(s) 325, 463, 494, 1272
GluI GCNGC 5 cut(s) 650, 653, 933, 1139, 1479
GsaI CCCAGC 1 cut(s) 11
GsuI CTGGAG 2 cut(s) 271, 500
HaeIII GGCC 1 cut(s) 1695
HapII CCGG 1 cut(s) 161
HgaI GACGC 2 cut(s) 113, 717
HhaI GCGC 4 cut(s) 326, 464, 495, 1273
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 6 cut(s) 104, 172, 446, 1318, 1331, 1477
Hin6I GCGC 4 cut(s) 324, 462, 493, 1271
HinP1I GCGC 4 cut(s) 324, 462, 493, 1271
HincII GTYRAC 5 cut(s) 39, 514, 541, 980, 1148
HindII GTYRAC 5 cut(s) 39, 514, 541, 980, 1148
HpaI GTTAAC 1 cut(s) 541
HpaII CCGG 1 cut(s) 161
HphI GGTGA 3 cut(s) 412, 674, 1145
Hpy166II GTNNAC 7 cut(s) 39, 514, 541, 980, 1148, 1323, 1627
Hpy188I TCNGA 9 cut(s) 79, 94, 208, 535, 640, 909, 1501, 1571, 1586
Hpy188III TCNNGA 6 cut(s) 145, 195, 337, 881, 1172, 1434
Hpy8I GTNNAC 7 cut(s) 39, 514, 541, 980, 1148, 1323, 1627
Hpy99I CGWCG 5 cut(s) 212, 269, 272, 275, 278
HpyAV CCTTC 6 cut(s) 517, 593, 879, 884, 1125, 1566
HpyCH4III ACNGT 3 cut(s) 1302, 1594, 1661
HpyCH4IV ACGT 1 cut(s) 367
HpyF10VI GCNNNNNNNGC 4 cut(s) 734, 929, 1384, 1393
HpySE526I ACGT 1 cut(s) 367
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 6 cut(s) 104, 172, 446, 1318, 1331, 1477
HspAI GCGC 4 cut(s) 324, 462, 493, 1271
KspAI GTTAAC 1 cut(s) 541
Kzo9I GATC 8 cut(s) 47, 141, 197, 355, 633, 679, 1174, 1334
LmnI GCTCC 5 cut(s) 705, 742, 781, 820, 1174
Lsp1109I GCAGC 5 cut(s) 636, 664, 944, 1150, 1465
LweI GCATC 3 cut(s) 322, 429, 1260
MaeI CTAG 3 cut(s) 195, 257, 1341
MaeII ACGT 1 cut(s) 367
MaeIII GTNAC 1 cut(s) 318
MalI GATC 8 cut(s) 49, 143, 199, 357, 635, 681, 1176, 1336
MboI GATC 8 cut(s) 47, 141, 197, 355, 633, 679, 1174, 1334
MboII GAAGA 1 cut(s) 424
MflI RGATCY 2 cut(s) 197, 633
MlsI TGGCCA 1 cut(s) 1695
MluCI AATT 5 cut(s) 29, 110, 433, 505, 1345
MluNI TGGCCA 1 cut(s) 1695
MlyI GAGTC 4 cut(s) 477, 478, 1153, 1505
MmeI TCCRAC 2 cut(s) 231, 672
Mox20I TGGCCA 1 cut(s) 1695
Mph1103I ATGCAT 2 cut(s) 444, 1468
MscI TGGCCA 1 cut(s) 1695
MseI TTAA 3 cut(s) 540, 675, 1632
Msp20I TGGCCA 1 cut(s) 1695
MspA1I CMGCKG 2 cut(s) 478, 649
MspCI CTTAAG 1 cut(s) 674
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 4 cut(s) 379, 497, 1275, 1368
MvaI CCWGG 4 cut(s) 379, 497, 1275, 1368
MvnI CGCG 3 cut(s) 324, 728, 1524
MwoI GCNNNNNNNGC 4 cut(s) 734, 929, 1384, 1393
NcoI CCATGG 1 cut(s) 1473
NdeII GATC 8 cut(s) 47, 141, 197, 355, 633, 679, 1174, 1334
NlaIII CATG 6 cut(s) 104, 172, 446, 1318, 1331, 1477
NlaIV GGNNCC 2 cut(s) 1005, 1312
NmeAIII GCCGAG 1 cut(s) 980
NmuCI GTSAC 1 cut(s) 318
NsiI ATGCAT 2 cut(s) 444, 1468
NspI RCATGY 1 cut(s) 446
PaeI GCATGC 1 cut(s) 446
PaeR7I CTCGAG 1 cut(s) 335
PaqCI CACCTGC 1 cut(s) 1164
PcsI WCGNNNNNNNCGW 3 cut(s) 143, 270, 273
PfeI GAWTC 7 cut(s) 87, 340, 989, 1015, 1198, 1502, 1566
Pfl23II CGTACG 1 cut(s) 307
PflFI GACNNNGTC 1 cut(s) 1580
PflMI CCANNNNNTGG 1 cut(s) 691
PkrI GCNGC 5 cut(s) 651, 654, 934, 1140, 1480
PleI GAGTC 4 cut(s) 476, 478, 1152, 1504
PpsI GAGTC 4 cut(s) 476, 478, 1152, 1504
Psp6I CCWGG 4 cut(s) 377, 495, 1273, 1366
PspFI CCCAGC 1 cut(s) 7
PspGI CCWGG 4 cut(s) 377, 495, 1273, 1366
PspLI CGTACG 1 cut(s) 307
PspN4I GGNNCC 2 cut(s) 1005, 1312
PspPI GGNCC 1 cut(s) 1627
PstI CTGCAG 1 cut(s) 654
PstNI CAGNNNCTG 1 cut(s) 1505
PsuI RGATCY 2 cut(s) 197, 633
PsyI GACNNNGTC 1 cut(s) 1580
PvuII CAGCTG 2 cut(s) 478, 649
RsaI GTAC 3 cut(s) 309, 861, 1107
RsaNI GTAC 3 cut(s) 308, 860, 1106
SaqAI TTAA 3 cut(s) 540, 675, 1632
SatI GCNGC 5 cut(s) 650, 653, 933, 1139, 1479
Sau3AI GATC 8 cut(s) 47, 141, 197, 355, 633, 679, 1174, 1334
Sau96I GGNCC 1 cut(s) 1627
SchI GAGTC 4 cut(s) 477, 478, 1153, 1505
ScrFI CCNGG 4 cut(s) 379, 497, 1275, 1368
SfaNI GCATC 3 cut(s) 322, 429, 1260
SfcI CTRYAG 2 cut(s) 650, 927
Sfr274I CTCGAG 1 cut(s) 335
SinI GGWCC 1 cut(s) 1627
SlaI CTCGAG 1 cut(s) 335
SmlI CTYRAG 4 cut(s) 335, 674, 1050, 1432
SmoI CTYRAG 4 cut(s) 335, 674, 1050, 1432
SphI GCATGC 1 cut(s) 446
Sse9I AATT 5 cut(s) 29, 110, 433, 505, 1345
SsiI CCGC 4 cut(s) 301, 726, 1509, 1522
SspMI CTAG 3 cut(s) 195, 257, 1341
StyD4I CCNGG 4 cut(s) 377, 495, 1273, 1366
StyI CCWWGG 1 cut(s) 1473
TaaI ACNGT 3 cut(s) 1302, 1594, 1661
TaiI ACGT 1 cut(s) 370
TaqI TCGA 3 cut(s) 46, 336, 1196
TaqII GACCGA 1 cut(s) 1347
TasI AATT 5 cut(s) 29, 110, 433, 505, 1345
TatI WGTACW 1 cut(s) 859
TfiI GAWTC 7 cut(s) 87, 340, 989, 1015, 1198, 1502, 1566
Tru1I TTAA 3 cut(s) 540, 675, 1632
Tru9I TTAA 3 cut(s) 540, 675, 1632
TscAI CASTG 5 cut(s) 453, 1489, 1599, 1629, 1666
TseFI GTSAC 1 cut(s) 318
TseI GCWGC 5 cut(s) 649, 652, 932, 1138, 1478
Tsp45I GTSAC 1 cut(s) 318
TspDTI ATGAA 5 cut(s) 157, 284, 431, 1182, 1670
TspRI CASTG 5 cut(s) 453, 1489, 1599, 1629, 1666
Tth111I GACNNNGTC 1 cut(s) 1580
Van91I CCANNNNNTGG 1 cut(s) 691
Vha464I CTTAAG 1 cut(s) 674
VpaK11BI GGWCC 1 cut(s) 1627
XapI RAATTY 1 cut(s) 505
XbaI TCTAGA 1 cut(s) 194
XceI RCATGY 1 cut(s) 446
XhoI CTCGAG 1 cut(s) 335
XmiI GTMKAC 1 cut(s) 1322
XspI CTAG 3 cut(s) 195, 257, 1341
Zsp2I ATGCAT 2 cut(s) 444, 1468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.