Rmu_sc0002040.1_g000061

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002040.1
OG0013667
Physical Location & Seq
Reverse (-)
261852 .. 264437
2586 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002040.1_g000061.1.cds

Sequence Viewer

Length: 1602 bp
atgagaacaacgtcggagttcatggacaagcagattatggaactttctagatcctactccgacgacttctctcagctcacataccctcctcatcctcatcctcatcagctagaaaacgacgacgacgacgacgacgacgacaacttgctctccagctttcatttccgccccgtacggcagcagtcacgcgcctccacgctcgagattcgtggttctatggatcaaactgaacgtgcgaaatccagggacttggcgatgatttcggtgattgatgggaagatgaaccagcattttggcaatttgatgcatgcggtggagggtctcagcgcacgagtctctcagctggagactcggttgcgcctggtagagaattctgttgacgacttgaaggattgttctgatgttaactatggcaagactgatgggaagctgagggaagtagaacacattttgagaaaggttgaaggtggtatgcaggatttgagagataagcaagagatctcagaggctcagctgcagcttgcaaaacttcaaatgcttaaggatcaccagaagttggaacaagaaaggagcaatgtgcaaacaacttccgcgtcatctgctcctcagaaatcccaccaatcatattcaactcctgttgctcctcatcaatcccaccaatcatattcaactcctgttgcttctgtacaacatccttcccttcctcctgatattgccccaaccctgacccatcagaactttccaccagcacctacagcagctcagcttccagctcagtcatctcagtatcaaactccttatagtcaacagccagattctaactactcggcacctgtgctgaatccacccacaactcaggcatactatatgtctcaagttcagcagtcgcaaccacctcccacggcaccatatcaaccctatccagcagtaccacatccttcacaaacatcacattttcagaagcagagtcaacttcacccgcctctcaatgctcctgatccacaagcccagtattcatcgattcacaatccagcagaaaccccgtatgggttatctcaaagctatccccagagcatccacaaaacctctaatgtgcctggcatggttctaccatcacaacaacagtttgagggttccatgcagtatacacatgaccgatctactagaaattacagcacagacttctcccctggaaatatgcaaccagttcaacgttcaagctacgatgacttttattcccacggcggatctccttctcactacagtgattcaaaaatgaaacagtcgcaagtatcaccatcctcttcggtcgcttctggtggaagcagtttctcaagactgccaactgcaaaggtattgccgtatgcattacccatggctgccagtgtggataaggagtcagattctggcgggactggaaaccgtgttccagttgataatgtcattgataaggttgtagctatgggattccgaagggacatagtcagagcaacagtgaggaaaatgactgagaatgggcaggcagtggaccttaatgttgtgctggataaactgatgaacagtggagaagttcagtcccaaagtggtggatttggccagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1421

Protein Analysis

533

Amino Acids

59.05

Weight (kDa)

5.94

Isoelectric Point (pI)

65.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013667)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05870 FvH4_5g05870
malus_domestica MD06G1169700.v1.1 MD14G1175200.v1.1
prunus_persica Prupe.5G168500_v2.0.a1
pyrus_communis pycom06g15100 pycom14g14620
rosa_chinensis RchiOBHm_Chr7g0192951
rosa_laevigata RLG00000004309
rosa_multiflora Rmu_sc0002040.1_g000061
rosa_roxburghii Rroxscaffold_3G00262510
rosa_rugosa Rorug07G0006400 Rorug07G0294800
rosa_samantha Rh7AG131600 Rh7BG132500 Rh7CG135700 Rh7DG133600
rosa_wichuraiana Rw7G011230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 829, 904
AccB7I CCANNNNNTGG 1 cut(s) 556
AccI GTMKAC 1 cut(s) 1145
AccII CGCG 2 cut(s) 189, 593
AciI CCGC 6 cut(s) 166, 311, 591, 980, 1245, 1410
AclI AACGTT 1 cut(s) 1213
AclWI GGATC 5 cut(s) 45, 228, 552, 992, 1255
AcoI YGGCCR 1 cut(s) 1594
AcsI RAATTY 1 cut(s) 370
AfaI GTAC 3 cut(s) 174, 687, 930
AfiI CCNNNNNNNGG 2 cut(s) 556, 1047
AflII CTTAAG 1 cut(s) 539
AgsI TTSAA 8 cut(s) 388, 464, 533, 630, 669, 1211, 1218, 1272
AjnI CCWGG 4 cut(s) 242, 360, 1096, 1189
AleI CACNNNNGTG 1 cut(s) 1263
AloI GAACNNNNNNTCC 2 cut(s) 1410, 1442
Alw26I GTCTC 4 cut(s) 326, 340, 341, 876
AlwI GGATC 5 cut(s) 45, 228, 552, 992, 1255
AlwNI CAGNNNCTG 1 cut(s) 1406
Ama87I CYCGRG 1 cut(s) 200
AoxI GGCC 1 cut(s) 1594
ApeKI GCWGC 5 cut(s) 178, 514, 517, 758, 1379
ApoI RAATTY 1 cut(s) 370
AspLEI GCGC 3 cut(s) 191, 329, 360
AspS9I GGNCC 1 cut(s) 1528
AsuHPI GGTGA 4 cut(s) 277, 539, 968, 1287
AvaI CYCGRG 1 cut(s) 200
AvaII GGWCC 1 cut(s) 1528
BalI TGGCCA 1 cut(s) 1596
BanI GGYRCC 2 cut(s) 829, 904
BauI CACGAG 1 cut(s) 330
BbvCI CCTCAGC 1 cut(s) 431
BbvI GCAGC 5 cut(s) 190, 501, 529, 770, 1366
BccI CCATC 5 cut(s) 266, 416, 738, 1120, 1306
BceAI ACGGC 4 cut(s) 191, 918, 1258, 1345
BciT130I CCWGG 4 cut(s) 244, 362, 1098, 1191
BcoDI GTCTC 4 cut(s) 326, 340, 341, 876
BfaI CTAG 3 cut(s) 48, 110, 1164
BfmI CTRYAG 3 cut(s) 515, 753, 1261
BfrI CTTAAG 1 cut(s) 539
BglII AGATCT 1 cut(s) 498
BisI GCNGC 5 cut(s) 179, 515, 518, 759, 1380
BlpI GCTNAGC 2 cut(s) 510, 762
BlsI GCNGC 5 cut(s) 180, 516, 519, 760, 1381
Bme1390I CCNGG 4 cut(s) 244, 362, 1098, 1191
Bme18I GGWCC 1 cut(s) 1528
BmeT110I CYCGRG 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 1528
BmiI GGNNCC 3 cut(s) 831, 906, 1135
BmrFI CCNGG 4 cut(s) 244, 362, 1098, 1191
BmrI ACTGGG 1 cut(s) 1003
BmsI GCATC 2 cut(s) 294, 1083
BmuI ACTGGG 1 cut(s) 1003
BpmI CTGGAG 2 cut(s) 136, 365
Bpu10I CCTNAGC 1 cut(s) 431
Bpu1102I GCTNAGC 2 cut(s) 510, 762
BpuEI CTTGAG 2 cut(s) 858, 1318
Bsa29I ATCGAT 1 cut(s) 1019
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 5 cut(s) 243, 900, 1189, 1240, 1374
BsaXI ACNNNNNCTCC 6 cut(s) 70, 100, 134, 164, 338, 368
Bsc4I CCNNNNNNNGG 2 cut(s) 556, 1047
Bse1I ACTGG 6 cut(s) 1009, 1205, 1383, 1420, 1430, 1597
Bse3DI GCAATG 1 cut(s) 580
BseBI CCWGG 4 cut(s) 244, 362, 1098, 1191
BseCI ATCGAT 1 cut(s) 1019
BseDI CCNNGG 5 cut(s) 243, 900, 1189, 1240, 1374
BseGI GGATG 6 cut(s) 91, 97, 691, 934, 1074, 1298
BseLI CCNNNNNNNGG 2 cut(s) 556, 1047
BseMI GCAATG 1 cut(s) 580
BseNI ACTGG 6 cut(s) 1009, 1205, 1383, 1420, 1430, 1597
BseRI GAGGAG 3 cut(s) 78, 594, 633
BseXI GCAGC 5 cut(s) 190, 501, 529, 770, 1366
Bsh1236I CGCG 2 cut(s) 189, 593
Bsh1285I CGRYCG 1 cut(s) 1311
BshFI GGCC 1 cut(s) 1596
BshNI GGYRCC 2 cut(s) 829, 904
BshVI ATCGAT 1 cut(s) 1019
BsiEI CGRYCG 1 cut(s) 1311
BsiHKCI CYCGRG 1 cut(s) 200
BsiWI CGTACG 1 cut(s) 172
BslFI GGGAC 4 cut(s) 260, 1426, 1490, 1561
BslI CCNNNNNNNGG 2 cut(s) 556, 1047
BsmAI GTCTC 4 cut(s) 326, 340, 341, 876
BsmFI GGGAC 4 cut(s) 260, 1426, 1490, 1561
BsnI GGCC 1 cut(s) 1596
Bso31I GGTCTC 1 cut(s) 326
BsoBI CYCGRG 1 cut(s) 200
Bsp1407I TGTACA 1 cut(s) 685
Bsp143I GATC 7 cut(s) 50, 220, 498, 544, 997, 1157, 1247
Bsp1720I GCTNAGC 2 cut(s) 510, 762
Bsp19I CCATGG 1 cut(s) 1374
BspACI CCGC 6 cut(s) 166, 311, 591, 980, 1245, 1410
BspANI GGCC 1 cut(s) 1596
BspDI ATCGAT 1 cut(s) 1019
BspFNI CGCG 2 cut(s) 189, 593
BspLI GGNNCC 3 cut(s) 831, 906, 1135
BspMAI CTGCAG 1 cut(s) 519
BspPI GGATC 5 cut(s) 45, 228, 552, 992, 1255
BspT107I GGYRCC 2 cut(s) 829, 904
BspTI CTTAAG 1 cut(s) 539
BspTNI GGTCTC 1 cut(s) 326
BsrDI GCAATG 1 cut(s) 580
BsrGI TGTACA 1 cut(s) 685
BsrI ACTGG 6 cut(s) 1009, 1205, 1383, 1420, 1430, 1597
BssECI CCNNGG 5 cut(s) 243, 900, 1189, 1240, 1374
BssMI GATC 7 cut(s) 50, 220, 498, 544, 997, 1157, 1247
BssNAI GTATAC 1 cut(s) 1146
BssSI CACGAG 1 cut(s) 330
BssT1I CCWWGG 1 cut(s) 1374
Bst1107I GTATAC 1 cut(s) 1146
Bst2BI CACGAG 1 cut(s) 330
Bst2UI CCWGG 4 cut(s) 244, 362, 1098, 1191
Bst4CI ACNGT 6 cut(s) 1125, 1265, 1284, 1424, 1495, 1562
Bst6I CTCTTC 1 cut(s) 1309
BstAFI CTTAAG 1 cut(s) 539
BstAUI TGTACA 1 cut(s) 685
BstC8I GCNNGC 3 cut(s) 309, 522, 1521
BstDSI CCRYGG 3 cut(s) 900, 1240, 1374
BstF5I GGATG 6 cut(s) 91, 97, 691, 934, 1074, 1298
BstFNI CGCG 2 cut(s) 189, 593
BstHHI GCGC 3 cut(s) 191, 329, 360
BstKTI GATC 7 cut(s) 53, 223, 501, 547, 1000, 1160, 1250
BstMAI GTCTC 4 cut(s) 326, 340, 341, 876
BstMBI GATC 7 cut(s) 50, 220, 498, 544, 997, 1157, 1247
BstMCI CGRYCG 1 cut(s) 1311
BstMWI GCNNNNNNNGC 2 cut(s) 599, 755
BstNI CCWGG 4 cut(s) 244, 362, 1098, 1191
BstNSI RCATGY 1 cut(s) 311
BstSCI CCNGG 4 cut(s) 242, 360, 1096, 1189
BstSFI CTRYAG 3 cut(s) 515, 753, 1261
BstUI CGCG 2 cut(s) 189, 593
BstV1I GCAGC 5 cut(s) 190, 501, 529, 770, 1366
BstX2I RGATCY 3 cut(s) 50, 498, 1247
BstXI CCANNNNNNTGG 3 cut(s) 250, 293, 1586
BstYI RGATCY 3 cut(s) 50, 498, 1247
BstZ17I GTATAC 1 cut(s) 1146
Bsu15I ATCGAT 1 cut(s) 1019
BsuRI GGCC 1 cut(s) 1596
BsuTUI ATCGAT 1 cut(s) 1019
BtgI CCRYGG 3 cut(s) 900, 1240, 1374
BtgZI GCGATG 1 cut(s) 269
BtsCI GGATG 6 cut(s) 91, 97, 691, 934, 1074, 1298
BtsI GCAGTG 1 cut(s) 1530
BtsIMutI CAGTG 5 cut(s) 1270, 1390, 1500, 1530, 1567
Cac8I GCNNGC 3 cut(s) 309, 522, 1521
CaiI CAGNNNCTG 1 cut(s) 1406
CfoI GCGC 3 cut(s) 191, 329, 360
Cfr13I GGNCC 1 cut(s) 1528
ClaI ATCGAT 1 cut(s) 1019
CseI GACGC 1 cut(s) 582
Csp6I GTAC 3 cut(s) 173, 686, 929
CviAII CATG 6 cut(s) 22, 308, 1102, 1138, 1151, 1375
CviQI GTAC 3 cut(s) 173, 686, 929
DpnI GATC 7 cut(s) 52, 222, 500, 546, 999, 1159, 1249
DpnII GATC 7 cut(s) 50, 220, 498, 544, 997, 1157, 1247
EaeI YGGCCR 1 cut(s) 1594
Eam1104I CTCTTC 1 cut(s) 1309
EarI CTCTTC 1 cut(s) 1309
EciI GGCGGA 2 cut(s) 155, 1260
Eco130I CCWWGG 1 cut(s) 1374
Eco31I GGTCTC 1 cut(s) 326
Eco47I GGWCC 1 cut(s) 1528
Eco88I CYCGRG 1 cut(s) 200
EcoRI GAATTC 1 cut(s) 370
EcoRII CCWGG 4 cut(s) 242, 360, 1096, 1189
EcoT14I CCWWGG 1 cut(s) 1374
EcoT22I ATGCAT 2 cut(s) 309, 1369
ErhI CCWWGG 1 cut(s) 1374
FaeI CATG 6 cut(s) 25, 311, 1105, 1141, 1154, 1378
FaqI GGGAC 4 cut(s) 260, 1426, 1490, 1561
FatI CATG 6 cut(s) 21, 307, 1101, 1137, 1150, 1374
FauI CCCGC 2 cut(s) 987, 1403
FblI GTMKAC 1 cut(s) 1145
Fnu4HI GCNGC 5 cut(s) 179, 515, 518, 759, 1380
FokI GGATG 6 cut(s) 78, 84, 678, 921, 1061, 1285
Fsp4HI GCNGC 5 cut(s) 179, 515, 518, 759, 1380
FspBI CTAG 3 cut(s) 48, 110, 1164
GlaI GCGC 3 cut(s) 190, 328, 359
GluI GCNGC 5 cut(s) 179, 515, 518, 759, 1380
GsuI CTGGAG 2 cut(s) 136, 365
HaeIII GGCC 1 cut(s) 1596
HgaI GACGC 1 cut(s) 582
HhaI GCGC 3 cut(s) 191, 329, 360
Hin1II CATG 6 cut(s) 25, 311, 1105, 1141, 1154, 1378
Hin6I GCGC 3 cut(s) 189, 327, 358
HinP1I GCGC 3 cut(s) 189, 327, 358
HincII GTYRAC 4 cut(s) 379, 406, 806, 971
HindII GTYRAC 4 cut(s) 379, 406, 806, 971
HpaI GTTAAC 1 cut(s) 406
HphI GGTGA 4 cut(s) 277, 539, 968, 1287
Hpy166II GTNNAC 6 cut(s) 379, 406, 806, 971, 1146, 1528
Hpy188III TCNNGA 5 cut(s) 48, 202, 707, 995, 1335
Hpy8I GTNNAC 6 cut(s) 379, 406, 806, 971, 1146, 1528
Hpy99I CGWCG 9 cut(s) 16, 65, 122, 125, 128, 131, 134, 137, 140
HpyAV CCTTC 7 cut(s) 382, 458, 705, 710, 948, 1263, 1467
HpyCH4III ACNGT 6 cut(s) 1125, 1265, 1284, 1424, 1495, 1562
HpyCH4IV ACGT 3 cut(s) 11, 232, 1213
HpyCH4V TGCA 9 cut(s) 307, 475, 517, 524, 580, 1141, 1201, 1349, 1367
HpyF10VI GCNNNNNNNGC 2 cut(s) 599, 755
HpySE526I ACGT 3 cut(s) 11, 232, 1213
Hsp92II CATG 6 cut(s) 25, 311, 1105, 1141, 1154, 1378
HspAI GCGC 3 cut(s) 189, 327, 358
KspAI GTTAAC 1 cut(s) 406
Kzo9I GATC 7 cut(s) 50, 220, 498, 544, 997, 1157, 1247
LmnI GCTCC 4 cut(s) 570, 607, 646, 997
Lsp1109I GCAGC 5 cut(s) 190, 501, 529, 770, 1366
LweI GCATC 2 cut(s) 294, 1083
MaeI CTAG 3 cut(s) 48, 110, 1164
MaeII ACGT 3 cut(s) 11, 232, 1213
MaeIII GTNAC 1 cut(s) 183
MalI GATC 7 cut(s) 52, 222, 500, 546, 999, 1159, 1249
MboI GATC 7 cut(s) 50, 220, 498, 544, 997, 1157, 1247
MboII GAAGA 2 cut(s) 289, 1296
MflI RGATCY 3 cut(s) 50, 498, 1247
MlsI TGGCCA 1 cut(s) 1596
MluCI AATT 3 cut(s) 298, 370, 1168
MluNI TGGCCA 1 cut(s) 1596
MlyI GAGTC 4 cut(s) 342, 343, 976, 1406
MmeI TCCRAC 2 cut(s) 84, 537
Mox20I TGGCCA 1 cut(s) 1596
Mph1103I ATGCAT 2 cut(s) 309, 1369
MscI TGGCCA 1 cut(s) 1596
MseI TTAA 3 cut(s) 405, 540, 1533
MslI CAYNNNNRTG 1 cut(s) 1263
Msp20I TGGCCA 1 cut(s) 1596
MspA1I CMGCKG 2 cut(s) 343, 514
MspCI CTTAAG 1 cut(s) 539
MspR9I CCNGG 4 cut(s) 244, 362, 1098, 1191
MvaI CCWGG 4 cut(s) 244, 362, 1098, 1191
MvnI CGCG 2 cut(s) 189, 593
MwoI GCNNNNNNNGC 2 cut(s) 599, 755
NcoI CCATGG 1 cut(s) 1374
NdeII GATC 7 cut(s) 50, 220, 498, 544, 997, 1157, 1247
NlaIII CATG 6 cut(s) 25, 311, 1105, 1141, 1154, 1378
NlaIV GGNNCC 3 cut(s) 831, 906, 1135
NmeAIII GCCGAG 1 cut(s) 806
NmuCI GTSAC 1 cut(s) 183
NsiI ATGCAT 2 cut(s) 309, 1369
NspI RCATGY 1 cut(s) 311
OliI CACNNNNGTG 1 cut(s) 1263
PaeI GCATGC 1 cut(s) 311
PaeR7I CTCGAG 1 cut(s) 200
PcsI WCGNNNNNNNCGW 5 cut(s) 123, 126, 129, 132, 135
PfeI GAWTC 7 cut(s) 205, 815, 841, 1021, 1268, 1403, 1467
Pfl23II CGTACG 1 cut(s) 172
PflFI GACNNNGTC 1 cut(s) 1481
PflMI CCANNNNNTGG 1 cut(s) 556
PkrI GCNGC 5 cut(s) 180, 516, 519, 760, 1381
PleI GAGTC 4 cut(s) 341, 343, 975, 1405
PpsI GAGTC 4 cut(s) 341, 343, 975, 1405
Psp1406I AACGTT 1 cut(s) 1213
Psp6I CCWGG 4 cut(s) 242, 360, 1096, 1189
PspGI CCWGG 4 cut(s) 242, 360, 1096, 1189
PspLI CGTACG 1 cut(s) 172
PspN4I GGNNCC 3 cut(s) 831, 906, 1135
PspPI GGNCC 1 cut(s) 1528
PstI CTGCAG 1 cut(s) 519
PstNI CAGNNNCTG 1 cut(s) 1406
PsuI RGATCY 3 cut(s) 50, 498, 1247
PsyI GACNNNGTC 1 cut(s) 1481
PvuII CAGCTG 2 cut(s) 343, 514
RsaI GTAC 3 cut(s) 174, 687, 930
RsaNI GTAC 3 cut(s) 173, 686, 929
RseI CAYNNNNRTG 1 cut(s) 1263
SaqAI TTAA 3 cut(s) 405, 540, 1533
SatI GCNGC 5 cut(s) 179, 515, 518, 759, 1380
Sau3AI GATC 7 cut(s) 50, 220, 498, 544, 997, 1157, 1247
Sau96I GGNCC 1 cut(s) 1528
SchI GAGTC 4 cut(s) 342, 343, 976, 1406
ScrFI CCNGG 4 cut(s) 244, 362, 1098, 1191
SfaNI GCATC 2 cut(s) 294, 1083
SfcI CTRYAG 3 cut(s) 515, 753, 1261
Sfr274I CTCGAG 1 cut(s) 200
SinI GGWCC 1 cut(s) 1528
SlaI CTCGAG 1 cut(s) 200
SmiMI CAYNNNNRTG 1 cut(s) 1263
SmlI CTYRAG 4 cut(s) 200, 539, 873, 1333
SmoI CTYRAG 4 cut(s) 200, 539, 873, 1333
SphI GCATGC 1 cut(s) 311
Sse9I AATT 3 cut(s) 298, 370, 1168
SsiI CCGC 6 cut(s) 166, 311, 591, 980, 1245, 1410
SspMI CTAG 3 cut(s) 48, 110, 1164
StyD4I CCNGG 4 cut(s) 242, 360, 1096, 1189
StyI CCWWGG 1 cut(s) 1374
TaaI ACNGT 6 cut(s) 1125, 1265, 1284, 1424, 1495, 1562
TaiI ACGT 3 cut(s) 14, 235, 1216
TaqI TCGA 2 cut(s) 201, 1019
TaqII GACCGA 2 cut(s) 1170, 1297
TasI AATT 3 cut(s) 298, 370, 1168
TatI WGTACW 1 cut(s) 685
TfiI GAWTC 7 cut(s) 205, 815, 841, 1021, 1268, 1403, 1467
Tru1I TTAA 3 cut(s) 405, 540, 1533
Tru9I TTAA 3 cut(s) 405, 540, 1533
TscAI CASTG 5 cut(s) 1270, 1390, 1500, 1530, 1567
TseFI GTSAC 1 cut(s) 183
TseI GCWGC 5 cut(s) 178, 514, 517, 758, 1379
Tsp45I GTSAC 1 cut(s) 183
TspDTI ATGAA 6 cut(s) 10, 149, 296, 1005, 1292, 1571
TspRI CASTG 5 cut(s) 1270, 1390, 1500, 1530, 1567
Tth111I GACNNNGTC 1 cut(s) 1481
Van91I CCANNNNNTGG 1 cut(s) 556
Vha464I CTTAAG 1 cut(s) 539
VpaK11BI GGWCC 1 cut(s) 1528
XapI RAATTY 1 cut(s) 370
XbaI TCTAGA 1 cut(s) 47
XceI RCATGY 1 cut(s) 311
XhoI CTCGAG 1 cut(s) 200
XmiI GTMKAC 1 cut(s) 1145
XspI CTAG 3 cut(s) 48, 110, 1164
Zsp2I ATGCAT 2 cut(s) 309, 1369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.