MD14G1182700.v1.1

helix loop helix domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007090
Physical Location & Seq
Forward (+)
27552212 .. 27553172
961 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1182700.v1.1.491

Sequence Viewer

Length: 564 bp
ATGAAGGGGTGTAACAATGAGTCTTCTTCCAAACCGGACAGGAAGACAGTGGAGAGAAACAGAAGAATTCACATGAAAAGTCTCTGCTTCAAGCTTGCTTCACTTGTTCCTCCCCAACACTTCAAAACCACCAATTCCAAGGGTACCATGTCACAGCAAAATCAGCTTGATACTGCGGCATCCTATATAAAGAAACTGAGAGAGAGAATCGAGAATCTGAAAGAGAGGAAGGAGAAAGCAATGAAGTCACAATTAGGCAGTTCTAAGTCTGACATTGGCTATATTGACCCAGAAAAAACCGCGATGATGGGCTCAAGATTGCCAGTTATTGAATTAAGAGACTCGGGTTCAAGCATAGAAGTGATGCTGATCAGTGGGTTGAATAAGAACTTCATGTTTTATGAAGTCATCAGTGTTCTTGAGGAAGAAGGAGCTGAAGTTGTCAGTGCTAGCTTTACCACCATAGGCGATAAAGTTTTCCACTCGGTGCATGCTCAGGTCAAAATTTCTAGAGTTGGAGTAGATACTTCAACGGTATTGCAGAGACTACAGGATTTGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

188

Amino Acids

20.99

Weight (kDa)

9.44

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 16 - 67 6.4e-06 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 143
AccII CGCG 1 cut(s) 302
AciI CCGC 2 cut(s) 176, 300
AcsI RAATTY 2 cut(s) 66, 504
AcuI CTGAAG 1 cut(s) 456
AdeI CACNNNGTG 1 cut(s) 487
AfaI GTAC 1 cut(s) 145
AgsI TTSAA 6 cut(s) 91, 124, 332, 351, 382, 531
AluBI AGCT 4 cut(s) 94, 166, 434, 453
AluI AGCT 4 cut(s) 94, 166, 434, 453
Alw26I GTCTC 3 cut(s) 86, 333, 538
Ama87I CYCGRG 1 cut(s) 343
ApoI RAATTY 2 cut(s) 66, 504
Asp718I GGTACC 1 cut(s) 143
AsuNHI GCTAGC 1 cut(s) 449
AvaI CYCGRG 1 cut(s) 343
BanI GGYRCC 1 cut(s) 143
BanII GRGCYC 1 cut(s) 314
BbsI GAAGAC 2 cut(s) 15, 50
BccI CCATC 1 cut(s) 301
BclI TGATCA 1 cut(s) 369
BcoDI GTCTC 3 cut(s) 86, 333, 538
BfaI CTAG 2 cut(s) 450, 510
BfmI CTRYAG 1 cut(s) 548
BisI GCNGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 178
BmeT110I CYCGRG 1 cut(s) 343
BmiI GGNNCC 1 cut(s) 145
BmsI GCATC 2 cut(s) 188, 354
BmtI GCTAGC 1 cut(s) 453
BpiI GAAGAC 2 cut(s) 15, 50
Bpu10I CCTNAGC 1 cut(s) 495
BpuEI CTTGAG 2 cut(s) 298, 440
BsaBI GATNNNNATC 1 cut(s) 368
BsaJI CCNNGG 1 cut(s) 138
BsaWI WCCGGW 1 cut(s) 34
BsaXI ACNNNNNCTCC 2 cut(s) 423, 453
Bse1I ACTGG 1 cut(s) 323
Bse3DI GCAATG 1 cut(s) 246
Bse8I GATNNNNATC 1 cut(s) 368
BseDI CCNNGG 1 cut(s) 138
BseGI GGATG 1 cut(s) 179
BseJI GATNNNNATC 1 cut(s) 368
BseMI GCAATG 1 cut(s) 246
BseMII CTCAG 2 cut(s) 188, 509
BseNI ACTGG 1 cut(s) 323
Bsh1236I CGCG 1 cut(s) 302
BshNI GGYRCC 1 cut(s) 143
BsiHKCI CYCGRG 1 cut(s) 343
BsiSI CCGG 1 cut(s) 35
BsmAI GTCTC 3 cut(s) 86, 333, 538
BsoBI CYCGRG 1 cut(s) 343
Bsp1286I GDGCHC 1 cut(s) 314
Bsp143I GATC 1 cut(s) 369
BspACI CCGC 2 cut(s) 176, 300
BspCNI CTCAG 2 cut(s) 189, 508
BspFNI CGCG 1 cut(s) 302
BspLI GGNNCC 1 cut(s) 145
BspOI GCTAGC 1 cut(s) 453
BspT107I GGYRCC 1 cut(s) 143
BsrDI GCAATG 1 cut(s) 246
BsrI ACTGG 1 cut(s) 323
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 1 cut(s) 369
BssT1I CCWWGG 1 cut(s) 138
Bst4CI ACNGT 2 cut(s) 49, 535
BstC8I GCNNGC 3 cut(s) 96, 451, 492
BstDEI CTNAG 3 cut(s) 197, 264, 495
BstF5I GGATG 1 cut(s) 179
BstFNI CGCG 1 cut(s) 302
BstKTI GATC 1 cut(s) 372
BstMAI GTCTC 3 cut(s) 86, 333, 538
BstMBI GATC 1 cut(s) 369
BstMWI GCNNNNNNNGC 1 cut(s) 163
BstNSI RCATGY 1 cut(s) 494
BstSFI CTRYAG 1 cut(s) 548
BstUI CGCG 1 cut(s) 302
BstV2I GAAGAC 2 cut(s) 15, 50
BtgZI GCGATG 1 cut(s) 317
BtsCI GGATG 1 cut(s) 179
BtsIMutI CAGTG 4 cut(s) 54, 379, 418, 451
Cac8I GCNNGC 3 cut(s) 96, 451, 492
Csp6I GTAC 1 cut(s) 144
CviAII CATG 4 cut(s) 73, 148, 394, 491
CviJI RGCY 6 cut(s) 94, 166, 279, 312, 434, 453
CviKI_1 RGCY 6 cut(s) 94, 166, 279, 312, 434, 453
CviQI GTAC 1 cut(s) 144
DdeI CTNAG 3 cut(s) 197, 264, 495
DpnI GATC 1 cut(s) 371
DpnII GATC 1 cut(s) 369
DraIII CACNNNGTG 1 cut(s) 487
Eco130I CCWWGG 1 cut(s) 138
Eco24I GRGCYC 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 456
Eco88I CYCGRG 1 cut(s) 343
EcoRI GAATTC 1 cut(s) 66
EcoT14I CCWWGG 1 cut(s) 138
EcoT38I GRGCYC 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 4 cut(s) 76, 151, 397, 494
FatI CATG 4 cut(s) 72, 147, 393, 490
FbaI TGATCA 1 cut(s) 369
Fnu4HI GCNGC 1 cut(s) 177
FokI GGATG 1 cut(s) 166
FriOI GRGCYC 1 cut(s) 314
Fsp4HI GCNGC 1 cut(s) 177
FspBI CTAG 2 cut(s) 450, 510
GluI GCNGC 1 cut(s) 177
HapII CCGG 1 cut(s) 35
Hin1II CATG 4 cut(s) 76, 151, 397, 494
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 4 cut(s) 20, 207, 214, 341
HpaII CCGG 1 cut(s) 35
Hpy188I TCNGA 2 cut(s) 219, 271
Hpy188III TCNNGA 4 cut(s) 211, 315, 419, 510
HpyAV CCTTC 2 cut(s) 223, 422
HpyCH4III ACNGT 2 cut(s) 49, 535
HpyCH4V TGCA 2 cut(s) 490, 541
HpyF10VI GCNNNNNNNGC 1 cut(s) 163
HpyF3I CTNAG 3 cut(s) 197, 264, 495
Hsp92II CATG 4 cut(s) 76, 151, 397, 494
KpnI GGTACC 1 cut(s) 147
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 1 cut(s) 369
LmnI GCTCC 1 cut(s) 431
LpnPI CCDG 6 cut(s) 25, 48, 303, 336, 482, 536
LweI GCATC 2 cut(s) 188, 354
MaeI CTAG 2 cut(s) 450, 510
MaeIII GTNAC 3 cut(s) 11, 150, 246
MalI GATC 1 cut(s) 371
MboI GATC 1 cut(s) 369
MboII GAAGA 5 cut(s) 15, 18, 55, 75, 437
MhlI GDGCHC 1 cut(s) 314
MluCI AATT 5 cut(s) 66, 133, 251, 332, 504
MlyI GAGTC 2 cut(s) 29, 335
MmeI TCCRAC 1 cut(s) 496
MnlI CCTC 3 cut(s) 120, 219, 415
MseI TTAA 1 cut(s) 335
MslI CAYNNNNRTG 1 cut(s) 359
MspI CCGG 1 cut(s) 35
MvnI CGCG 1 cut(s) 302
MwoI GCNNNNNNNGC 1 cut(s) 163
NdeII GATC 1 cut(s) 369
NheI GCTAGC 1 cut(s) 449
NlaIII CATG 4 cut(s) 76, 151, 397, 494
NlaIV GGNNCC 1 cut(s) 145
NmuCI GTSAC 2 cut(s) 150, 246
NspI RCATGY 1 cut(s) 494
PaeI GCATGC 1 cut(s) 494
PfeI GAWTC 2 cut(s) 207, 214
PkrI GCNGC 1 cut(s) 178
PleI GAGTC 2 cut(s) 28, 335
PpsI GAGTC 2 cut(s) 28, 335
PspN4I GGNNCC 1 cut(s) 145
RsaI GTAC 1 cut(s) 145
RsaNI GTAC 1 cut(s) 144
RseI CAYNNNNRTG 1 cut(s) 359
SaqAI TTAA 1 cut(s) 335
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 1 cut(s) 369
SchI GAGTC 2 cut(s) 29, 335
SduI GDGCHC 1 cut(s) 314
SetI ASST 5 cut(s) 96, 168, 436, 455, 501
SfaNI GCATC 2 cut(s) 188, 354
SfcI CTRYAG 1 cut(s) 548
SmiMI CAYNNNNRTG 1 cut(s) 359
SmlI CTYRAG 2 cut(s) 313, 419
SmoI CTYRAG 2 cut(s) 313, 419
SphI GCATGC 1 cut(s) 494
Sse9I AATT 5 cut(s) 66, 133, 251, 332, 504
SsiI CCGC 2 cut(s) 176, 300
SspMI CTAG 2 cut(s) 450, 510
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 2 cut(s) 49, 535
TaqI TCGA 1 cut(s) 210
TasI AATT 5 cut(s) 66, 133, 251, 332, 504
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 2 cut(s) 207, 214
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TscAI CASTG 4 cut(s) 54, 379, 418, 451
TseFI GTSAC 2 cut(s) 150, 246
Tsp45I GTSAC 2 cut(s) 150, 246
TspDTI ATGAA 5 cut(s) 17, 89, 257, 382, 417
TspRI CASTG 4 cut(s) 54, 379, 418, 451
XapI RAATTY 2 cut(s) 66, 504
XbaI TCTAGA 1 cut(s) 509
XceI RCATGY 1 cut(s) 494
XspI CTAG 2 cut(s) 450, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.