Prupe.5G175300_v2.0.a1

helix loop helix domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0007090
Physical Location & Seq
Forward (+)
14906726 .. 14908319
1594 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G175300.1

Sequence Viewer

Length: 561 bp
ATGAAGAAGTCCAGCGGTGAGTCTTCTTCCAAACTGGACAGGAAGACAGTGGAGAGGAACAGAAGAATCCACATGAAAGATCTCTGCTTCAAGCTTGCTTCCCTTATTCCTCCCCAACACTCCAAACCCTCCAACTCCAAGGACACGTTGTCGCAGCAAAATCAGTTCGATCTTGCGTCCTCCTACATAAAGCAACTGAGAGAGAGAATAGAGAATTTGAAGGAGAGGAAGGAGAAAGCAATGAGGTCACAATTAGGGATAGATGCTAATAGTACTATCGATGCCGATAATGCTATGATGGTGAGATCAAAATTGCCAGTTCTTGAATTAAGAGACTCAGGTTCAATTATAGAAGTGATGCTGATCAGTGGGTTGAACAAGAACTTCATGTTATATGAAGTGATTAGTGTTCTTGAGGAGGAAGGAGCAGAAGTTATCAGCGCTAGCTTTTCCACTATAGGAGATAAGGTTTTCCACTCGCTCCATGCTCAGGTCAAAATTTCTAGGGTTGGTGTTGAGACTTCAACTGTGTGGCAGAGACTGCAGGATTTGATTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

187

Amino Acids

21.08

Weight (kDa)

9.16

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 15
AcsI RAATTY 2 cut(s) 214, 498
AfaI GTAC 1 cut(s) 274
AfeI AGCGCT 1 cut(s) 442
AfiI CCNNNNNNNGG 1 cut(s) 490
AflIII ACRYGT 1 cut(s) 144
AgsI TTSAA 6 cut(s) 91, 220, 326, 345, 376, 525
AhdI GACNNNNNGTC 1 cut(s) 148
AluBI AGCT 2 cut(s) 94, 447
AluI AGCT 2 cut(s) 94, 447
Alw26I GTCTC 3 cut(s) 327, 512, 532
AlwNI CAGNNNCTG 1 cut(s) 541
Aor51HI AGCGCT 1 cut(s) 442
ApeKI GCWGC 1 cut(s) 154
ApoI RAATTY 2 cut(s) 214, 498
AspLEI GCGC 1 cut(s) 443
AsuHPI GGTGA 2 cut(s) 29, 313
AsuNHI GCTAGC 1 cut(s) 443
BbsI GAAGAC 2 cut(s) 15, 50
BbvI GCAGC 1 cut(s) 166
BccI CCATC 1 cut(s) 292
BclI TGATCA 1 cut(s) 363
BcoDI GTCTC 3 cut(s) 327, 512, 532
BfaI CTAG 2 cut(s) 444, 504
BfmI CTRYAG 2 cut(s) 456, 542
BfoI RGCGCY 1 cut(s) 444
BglII AGATCT 1 cut(s) 79
BisI GCNGC 1 cut(s) 155
BlsI GCNGC 1 cut(s) 156
BmcAI AGTACT 1 cut(s) 274
BmeRI GACNNNNNGTC 1 cut(s) 148
BmsI GCATC 3 cut(s) 253, 271, 348
BmtI GCTAGC 1 cut(s) 447
BpiI GAAGAC 2 cut(s) 15, 50
Bpu10I CCTNAGC 1 cut(s) 489
BpuEI CTTGAG 1 cut(s) 434
Bsa29I ATCGAT 1 cut(s) 279
BsaBI GATNNNNATC 1 cut(s) 362
BsaJI CCNNGG 1 cut(s) 138
BsaXI ACNNNNNCTCC 4 cut(s) 417, 447, 453, 483
Bsc4I CCNNNNNNNGG 1 cut(s) 490
Bse1I ACTGG 2 cut(s) 39, 317
Bse3DI GCAATG 1 cut(s) 246
Bse8I GATNNNNATC 1 cut(s) 362
BseCI ATCGAT 1 cut(s) 279
BseDI CCNNGG 1 cut(s) 138
BseJI GATNNNNATC 1 cut(s) 362
BseLI CCNNNNNNNGG 1 cut(s) 490
BseMI GCAATG 1 cut(s) 246
BseMII CTCAG 3 cut(s) 188, 351, 503
BseNI ACTGG 2 cut(s) 39, 317
BseRI GAGGAG 1 cut(s) 431
BseXI GCAGC 1 cut(s) 166
BshVI ATCGAT 1 cut(s) 279
BslI CCNNNNNNNGG 1 cut(s) 490
BsmAI GTCTC 3 cut(s) 327, 512, 532
Bsp143I GATC 4 cut(s) 79, 169, 305, 363
BspACI CCGC 1 cut(s) 15
BspCNI CTCAG 3 cut(s) 189, 350, 502
BspDI ATCGAT 1 cut(s) 279
BspMAI CTGCAG 1 cut(s) 546
BspOI GCTAGC 1 cut(s) 447
BsrDI GCAATG 1 cut(s) 246
BsrI ACTGG 2 cut(s) 39, 317
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 4 cut(s) 79, 169, 305, 363
BssT1I CCWWGG 1 cut(s) 138
Bst4CI ACNGT 2 cut(s) 49, 529
BstAPI GCANNNNNTGC 1 cut(s) 541
BstC8I GCNNGC 2 cut(s) 96, 445
BstDEI CTNAG 3 cut(s) 197, 337, 489
BstH2I RGCGCY 1 cut(s) 444
BstHHI GCGC 1 cut(s) 443
BstKTI GATC 4 cut(s) 82, 172, 308, 366
BstMAI GTCTC 3 cut(s) 327, 512, 532
BstMBI GATC 4 cut(s) 79, 169, 305, 363
BstMWI GCNNNNNNNGC 2 cut(s) 290, 541
BstSFI CTRYAG 2 cut(s) 456, 542
BstV1I GCAGC 1 cut(s) 166
BstV2I GAAGAC 2 cut(s) 15, 50
BstX2I RGATCY 1 cut(s) 79
BstYI RGATCY 1 cut(s) 79
Bsu15I ATCGAT 1 cut(s) 279
BsuTUI ATCGAT 1 cut(s) 279
BtsIMutI CAGTG 2 cut(s) 54, 373
Cac8I GCNNGC 2 cut(s) 96, 445
CaiI CAGNNNCTG 1 cut(s) 541
CfoI GCGC 1 cut(s) 443
ClaI ATCGAT 1 cut(s) 279
CseI GACGC 1 cut(s) 165
Csp6I GTAC 1 cut(s) 273
CviAII CATG 3 cut(s) 73, 388, 485
CviJI RGCY 2 cut(s) 94, 447
CviKI_1 RGCY 2 cut(s) 94, 447
CviQI GTAC 1 cut(s) 273
DdeI CTNAG 3 cut(s) 197, 337, 489
DpnI GATC 4 cut(s) 81, 171, 307, 365
DpnII GATC 4 cut(s) 79, 169, 305, 363
DriI GACNNNNNGTC 1 cut(s) 148
Eam1105I GACNNNNNGTC 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 138
Eco47III AGCGCT 1 cut(s) 442
EcoT14I CCWWGG 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 3 cut(s) 76, 391, 488
FaiI YATR 9 cut(s) 74, 188, 296, 350, 389, 394, 396, 458, 486
FatI CATG 3 cut(s) 72, 387, 484
FbaI TGATCA 1 cut(s) 363
Fnu4HI GCNGC 1 cut(s) 155
Fsp4HI GCNGC 1 cut(s) 155
FspBI CTAG 2 cut(s) 444, 504
GlaI GCGC 1 cut(s) 442
GluI GCNGC 1 cut(s) 155
HaeII RGCGCY 1 cut(s) 444
HgaI GACGC 1 cut(s) 165
HhaI GCGC 1 cut(s) 443
Hin1II CATG 3 cut(s) 76, 391, 488
Hin6I GCGC 1 cut(s) 441
HinP1I GCGC 1 cut(s) 441
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 3 cut(s) 20, 66, 335
HphI GGTGA 2 cut(s) 29, 313
Hpy188III TCNNGA 2 cut(s) 323, 413
HpyAV CCTTC 3 cut(s) 214, 223, 416
HpyCH4III ACNGT 2 cut(s) 49, 529
HpyCH4IV ACGT 1 cut(s) 146
HpyCH4V TGCA 1 cut(s) 544
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 541
HpyF3I CTNAG 3 cut(s) 197, 337, 489
HpySE526I ACGT 1 cut(s) 146
Hsp92II CATG 3 cut(s) 76, 391, 488
HspAI GCGC 1 cut(s) 441
Ksp22I TGATCA 1 cut(s) 363
Kzo9I GATC 4 cut(s) 79, 169, 305, 363
LmnI GCTCC 2 cut(s) 425, 486
LpnPI CCDG 7 cut(s) 20, 25, 25, 324, 330, 476, 530
Lsp1109I GCAGC 1 cut(s) 166
LweI GCATC 3 cut(s) 253, 271, 348
MaeI CTAG 2 cut(s) 444, 504
MaeII ACGT 1 cut(s) 146
MaeIII GTNAC 1 cut(s) 246
MalI GATC 4 cut(s) 81, 171, 307, 365
MboI GATC 4 cut(s) 79, 169, 305, 363
MboII GAAGA 5 cut(s) 15, 16, 18, 55, 75
MflI RGATCY 1 cut(s) 79
MluCI AATT 6 cut(s) 214, 251, 311, 326, 345, 498
MlyI GAGTC 2 cut(s) 29, 329
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 8 cut(s) 48, 120, 139, 190, 219, 237, 409, 412
MseI TTAA 1 cut(s) 329
MspA1I CMGCKG 1 cut(s) 15
MwoI GCNNNNNNNGC 2 cut(s) 290, 541
NdeII GATC 4 cut(s) 79, 169, 305, 363
NheI GCTAGC 1 cut(s) 443
NlaIII CATG 3 cut(s) 76, 391, 488
NmuCI GTSAC 1 cut(s) 246
PfeI GAWTC 1 cut(s) 66
PkrI GCNGC 1 cut(s) 156
PleI GAGTC 2 cut(s) 28, 329
PpsI GAGTC 2 cut(s) 28, 329
PstI CTGCAG 1 cut(s) 546
PstNI CAGNNNCTG 1 cut(s) 541
PsuI RGATCY 1 cut(s) 79
RsaI GTAC 1 cut(s) 274
RsaNI GTAC 1 cut(s) 273
SaqAI TTAA 1 cut(s) 329
SatI GCNGC 1 cut(s) 155
Sau3AI GATC 4 cut(s) 79, 169, 305, 363
ScaI AGTACT 1 cut(s) 274
SchI GAGTC 2 cut(s) 29, 329
SetI ASST 7 cut(s) 96, 149, 248, 343, 449, 471, 495
SfaNI GCATC 3 cut(s) 253, 271, 348
SfcI CTRYAG 2 cut(s) 456, 542
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
Sse9I AATT 6 cut(s) 214, 251, 311, 326, 345, 498
SsiI CCGC 1 cut(s) 15
SspMI CTAG 2 cut(s) 444, 504
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 2 cut(s) 49, 529
TaiI ACGT 1 cut(s) 149
TaqI TCGA 2 cut(s) 168, 279
TasI AATT 6 cut(s) 214, 251, 311, 326, 345, 498
TatI WGTACW 1 cut(s) 272
TfiI GAWTC 1 cut(s) 66
Tru1I TTAA 1 cut(s) 329
Tru9I TTAA 1 cut(s) 329
TscAI CASTG 2 cut(s) 54, 373
TseFI GTSAC 1 cut(s) 246
TseI GCWGC 1 cut(s) 154
Tsp45I GTSAC 1 cut(s) 246
TspDTI ATGAA 4 cut(s) 17, 89, 376, 411
TspRI CASTG 2 cut(s) 54, 373
XapI RAATTY 2 cut(s) 214, 498
XspI CTAG 2 cut(s) 444, 504
ZrmI AGTACT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.