Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0006594

Physical Location & Seq

Genomic Coordinates Reverse (-)

31728362 .. 31731452

Gene Structure 3091 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1238000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1191 bp

ATGGATGCTGCAAAGGTAGAAGAACTGAAGGAGTTCATCAGCAAGTGCAAGTCAAACCCTTCAATTGTTCACACCCCTTCTCTCGCCTTCTTCAAGTCATATCTCCAAAGCCTCGGCGCTCGGATTCCGCCGGTTCCGAAAATGGATAAAGATGATGGTGACATGTCAGACACGAAACAACATTTTGATGCCAAAAGTGATCCATCTGGGAATAACGATGACAATATTGTTGAATCTGATATTGAGTTGGATGTTACTGACGTTGTGGAGCCTGATAATGATCCTCCACAGAAGATGGGTGACCTGTCAATTGAAGTTACGGAAGAAATGCAGGATGCTGCACAAATAGAAAAATCAAAAGCTATCGATGCTGTCTCTGAAGGCATGGCTTCTTCTTGTTGTGAAACTGTAATTAGCATTTGTAGTAAGCTGGACGAAGCCATGGATCTTATAACAGAAGCCATAATGTTGAATCCAGCTTCAGCTATACTAAAAGCAACCAGAGCTAGTTTCTTTGTCAAATTGAATAAACCAAATGCGGCAATTCGTGATGCTAATGCAGCTTTAGAGATCAACCCCGACTCTGCAAAAGGATATAAAATAAGAGGAATGGCTAAGGCCATGTTGGGTCTATGGGAAGAAGCGGCGAGTGATCTGCATGTAGCATCAAAGTTAGATTATGATGAGGAGATTGGTTTGGTGCTTAAAAAGGTTGAACCTAATGTCCACAAAATTGAAGAGCATCGCAGAAAATATGAACGCCTGAGTAAAGAAAGGGAGATAAAAAGTGCTGAACAGGAGGGAAAGCGGAAAGCTGAAGCTCGAGAATGGGATGCGTTGTCTGCTCTTAAGGATGGGGAAGTCACTGGTATACACTCAGCCAGAGAATTAGAGACAAAGTTGAGTGCTGCTTCAAAGGCATCGCGCCTTGCAATCCTCTACTTCACCGCAACATGGTGTGGTCCTTGCCGCTTCATATCTCCTTTGTATACAAGCTTAGCTGGAAAGTACAAAAAAGTCGTTTTTTTGAAAGTTGACATTGATGAGGCAAGAGATGTGGCTGCAAACTGGAATATCAGCGGTGTTCCGGCCTTTTTCTTTGTAAGAAACGGTAAGGAGGTTGACAAGATGGTGGGGGCAGACAAAGCTGCACTAGAAAGGAAGATTGCGCAACATGCAGGCTCAATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0006457 GO:0006950 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0010286 GO:0016043 GO:0016491 GO:0016667 GO:0016671 GO:0022607 GO:0030544 GO:0031072 GO:0042802 GO:0043933 GO:0044085 GO:0050896 GO:0051259 GO:0055114 GO:0065003 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Thioredoxin

Protein Analysis

397

Amino Acids

43.57

Weight (kDa)

5.29

Isoelectric Point (pI)

35.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HipN	PF18253	2 - 43	7.1e-19	Hsp70-interacting protein N N-terminal domain
Thioredoxin	PF00085	305 - 390	2.4e-24	Thioredoxin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006594)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17880 AT3G17880
fragaria_vesca	FvH4_5g17540 FvH4_5g17540 FvH4_5g17540 FvH4_5g17540
malus_domestica	MD06G1228800.v1.1 MD14G1238000.v1.1
prunus_persica	Prupe.2G018600_v2.0.a1 Prupe.2G018600_v2.0.a1 Prupe.2G018600_v2.0.a1 Prupe.2G018700_v2.0.a1 Prupe.5G235200_v2.0.a1
pyrus_communis	pycom06g20510 pycom14g19910
rosa_chinensis	RchiOBHm_Chr7g0178111
rosa_laevigata	RLG00000005470
rosa_multiflora	Rmu_sc0000425.1_g000010
rosa_roxburghii	Rroxscaffold_3G00275170
rosa_rugosa	Rorug06G0413500 Rorug06G0413600
rosa_samantha	Rh7AG012900 Rh7BG012800 Rh7CG012800 Rh7DG013100
rosa_wichuraiana	Rw7G001020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	452
Acc16I	TGCGCA	1 cut(s)	1170
AccI	GTMKAC	2 cut(s)	871, 989
AccII	CGCG	1 cut(s)	925
AciI	CCGC	7 cut(s)	128, 539, 644, 808, 948, 970, 1080
AclWI	GGATC	3 cut(s)	194, 275, 453
AcuI	CTGAAG	4 cut(s)	47, 399, 465, 837
AfaI	GTAC	1 cut(s)	1010
AfiI	CCNNNNNNNGG	1 cut(s)	954
AflII	CTTAAG	1 cut(s)	848
AflIII	ACRYGT	1 cut(s)	162
AloI	GAACNNNNNNTCC	2 cut(s)	708, 740
Alw26I	GTCTC	2 cut(s)	379, 887
AlwI	GGATC	3 cut(s)	194, 275, 453
Ama87I	CYCGRG	1 cut(s)	822
AoxI	GGCC	2 cut(s)	618, 1089
ApeKI	GCWGC	6 cut(s)	8, 338, 560, 908, 1061, 1148
Asp700I	GAANNNNTTC	1 cut(s)	32
AspLEI	GCGC	3 cut(s)	119, 927, 1171
AspS9I	GGNCC	1 cut(s)	962
AsuHPI	GGTGA	3 cut(s)	170, 311, 937
AvaI	CYCGRG	1 cut(s)	822
AvaII	GGWCC	1 cut(s)	962
BbvI	GCAGC	5 cut(s)	325, 572, 895, 1048, 1135
BccI	CCATC	5 cut(s)	149, 211, 289, 848, 1123
BcgI	CGANNNNNNTGC	2 cut(s)	637, 671
BcoDI	GTCTC	2 cut(s)	379, 887
BfaI	CTAG	2 cut(s)	507, 1154
BfoI	RGCGCY	1 cut(s)	120
BfrI	CTTAAG	1 cut(s)	848
BisI	GCNGC	9 cut(s)	9, 339, 540, 561, 645, 909, 970, 1062, 1149
BlpI	GCTNAGC	1 cut(s)	997
BlsI	GCNGC	9 cut(s)	10, 340, 541, 562, 646, 910, 971, 1063, 1150
Bme18I	GGWCC	1 cut(s)	962
BmeT110I	CYCGRG	1 cut(s)	822
BmgT120I	GGNCC	1 cut(s)	962
BmiI	GGNNCC	2 cut(s)	135, 270
BmsI	GCATC	8 cut(s)	178, 325, 358, 541, 674, 751, 823, 929
Bpu10I	CCTNAGC	1 cut(s)	615
Bpu1102I	GCTNAGC	1 cut(s)	997
Bsa29I	ATCGAT	1 cut(s)	366
BsaBI	GATNNNNATC	1 cut(s)	279
BsaJI	CCNNGG	2 cut(s)	112, 441
Bsc4I	CCNNNNNNNGG	1 cut(s)	954
Bse118I	RCCGGY	1 cut(s)	130
Bse1I	ACTGG	2 cut(s)	871, 1073
Bse8I	GATNNNNATC	1 cut(s)	279
BseCI	ATCGAT	1 cut(s)	366
BseDI	CCNNGG	2 cut(s)	112, 441
BseGI	GGATG	5 cut(s)	10, 256, 340, 838, 859
BseJI	GATNNNNATC	1 cut(s)	279
BseLI	CCNNNNNNNGG	1 cut(s)	954
BseMII	CTCAG	2 cut(s)	755, 891
BseNI	ACTGG	2 cut(s)	871, 1073
BseRI	GAGGAG	1 cut(s)	701
BseXI	GCAGC	5 cut(s)	325, 572, 895, 1048, 1135
BsgI	GTGCAG	2 cut(s)	324, 1134
Bsh1236I	CGCG	1 cut(s)	925
BshFI	GGCC	2 cut(s)	620, 1091
BshVI	ATCGAT	1 cut(s)	366
BsiHKCI	CYCGRG	1 cut(s)	822
BsiSI	CCGG	2 cut(s)	131, 1088
BslI	CCNNNNNNNGG	1 cut(s)	954
BsmAI	GTCTC	2 cut(s)	379, 887
BsnI	GGCC	2 cut(s)	620, 1091
BsoBI	CYCGRG	1 cut(s)	822
Bsp143I	GATC	5 cut(s)	199, 280, 445, 570, 652
Bsp1720I	GCTNAGC	1 cut(s)	997
Bsp19I	CCATGG	1 cut(s)	441
BspACI	CCGC	7 cut(s)	128, 539, 644, 808, 948, 970, 1080
BspANI	GGCC	2 cut(s)	620, 1091
BspCNI	CTCAG	2 cut(s)	756, 890
BspDI	ATCGAT	1 cut(s)	366
BspFNI	CGCG	1 cut(s)	925
BspLI	GGNNCC	2 cut(s)	135, 270
BspPI	GGATC	3 cut(s)	194, 275, 453
BspQI	GCTCTTC	1 cut(s)	732
BspTI	CTTAAG	1 cut(s)	848
BsrFI	RCCGGY	1 cut(s)	130
BsrI	ACTGG	2 cut(s)	871, 1073
BssAI	RCCGGY	1 cut(s)	130
BssECI	CCNNGG	2 cut(s)	112, 441
BssMI	GATC	5 cut(s)	199, 280, 445, 570, 652
BssNAI	GTATAC	2 cut(s)	872, 990
BssT1I	CCWWGG	1 cut(s)	441
Bst1107I	GTATAC	2 cut(s)	872, 990
Bst4CI	ACNGT	2 cut(s)	409, 1112
Bst6I	CTCTTC	1 cut(s)	732
BstAFI	CTTAAG	1 cut(s)	848
BstC8I	GCNNGC	1 cut(s)	1180
BstDEI	CTNAG	4 cut(s)	615, 764, 877, 997
BstDSI	CCRYGG	1 cut(s)	441
BstEII	GGTNACC	1 cut(s)	299
BstF5I	GGATG	5 cut(s)	10, 256, 340, 838, 859
BstFNI	CGCG	1 cut(s)	925
BstH2I	RGCGCY	1 cut(s)	120
BstHHI	GCGC	3 cut(s)	119, 927, 1171
BstKTI	GATC	5 cut(s)	202, 283, 448, 573, 655
BstMAI	GTCTC	2 cut(s)	379, 887
BstMBI	GATC	5 cut(s)	199, 280, 445, 570, 652
BstMWI	GCNNNNNNNGC	7 cut(s)	368, 503, 560, 842, 917, 1145, 1175
BstNSI	RCATGY	3 cut(s)	166, 662, 1178
BstPI	GGTNACC	1 cut(s)	299
BstUI	CGCG	1 cut(s)	925
BstV1I	GCAGC	5 cut(s)	325, 572, 895, 1048, 1135
BstX2I	RGATCY	1 cut(s)	445
BstYI	RGATCY	1 cut(s)	445
BstZ17I	GTATAC	2 cut(s)	872, 990
Bsu15I	ATCGAT	1 cut(s)	366
BsuRI	GGCC	2 cut(s)	620, 1091
BsuTUI	ATCGAT	1 cut(s)	366
BtgI	CCRYGG	1 cut(s)	441
BtgZI	GCGATG	2 cut(s)	728, 906
BtsCI	GGATG	5 cut(s)	10, 256, 340, 838, 859
BtsIMutI	CAGTG	1 cut(s)	864
Cac8I	GCNNGC	1 cut(s)	1180
CfoI	GCGC	3 cut(s)	119, 927, 1171
Cfr10I	RCCGGY	1 cut(s)	130
Cfr13I	GGNCC	1 cut(s)	962
ClaI	ATCGAT	1 cut(s)	366
Csp6I	GTAC	1 cut(s)	1009
CspCI	CAANNNNNGTGG	2 cut(s)	1038, 1073
CviAII	CATG	7 cut(s)	163, 385, 442, 622, 659, 954, 1175
CviQI	GTAC	1 cut(s)	1009
DdeI	CTNAG	4 cut(s)	615, 764, 877, 997
DpnI	GATC	5 cut(s)	201, 282, 447, 572, 654
DpnII	GATC	5 cut(s)	199, 280, 445, 570, 652
Eam1104I	CTCTTC	1 cut(s)	732
EarI	CTCTTC	1 cut(s)	732
EciI	GGCGGA	1 cut(s)	117
Eco130I	CCWWGG	1 cut(s)	441
Eco47I	GGWCC	1 cut(s)	962
Eco57I	CTGAAG	4 cut(s)	47, 399, 465, 837
Eco88I	CYCGRG	1 cut(s)	822
Eco91I	GGTNACC	1 cut(s)	299
EcoO65I	GGTNACC	1 cut(s)	299
EcoT14I	CCWWGG	1 cut(s)	441
ErhI	CCWWGG	1 cut(s)	441
FaeI	CATG	7 cut(s)	166, 388, 445, 625, 662, 957, 1178
FatI	CATG	7 cut(s)	162, 384, 441, 621, 658, 953, 1174
FblI	GTMKAC	2 cut(s)	871, 989
Fnu4HI	GCNGC	9 cut(s)	9, 339, 540, 561, 645, 909, 970, 1062, 1149
FokI	GGATG	5 cut(s)	17, 263, 347, 845, 866
Fsp4HI	GCNGC	9 cut(s)	9, 339, 540, 561, 645, 909, 970, 1062, 1149
FspBI	CTAG	2 cut(s)	507, 1154
FspI	TGCGCA	1 cut(s)	1170
GlaI	GCGC	3 cut(s)	118, 926, 1170
GluI	GCNGC	9 cut(s)	9, 339, 540, 561, 645, 909, 970, 1062, 1149
HaeII	RGCGCY	1 cut(s)	120
HaeIII	GGCC	2 cut(s)	620, 1091
HapII	CCGG	2 cut(s)	131, 1088
HhaI	GCGC	3 cut(s)	119, 927, 1171
Hin1II	CATG	7 cut(s)	166, 388, 445, 625, 662, 957, 1178
Hin6I	GCGC	3 cut(s)	117, 925, 1169
HinP1I	GCGC	3 cut(s)	117, 925, 1169
HincII	GTYRAC	2 cut(s)	1036, 1123
HindII	GTYRAC	2 cut(s)	1036, 1123
HindIII	AAGCTT	1 cut(s)	994
HinfI	GANTC	4 cut(s)	124, 233, 472, 581
HpaII	CCGG	2 cut(s)	131, 1088
HphI	GGTGA	3 cut(s)	170, 311, 937
Hpy166II	GTNNAC	6 cut(s)	70, 727, 872, 990, 1036, 1123
Hpy188I	TCNGA	6 cut(s)	123, 138, 169, 238, 379, 1190
Hpy188III	TCNNGA	2 cut(s)	548, 824
Hpy8I	GTNNAC	6 cut(s)	70, 727, 872, 990, 1036, 1123
HpyAV	CCTTC	5 cut(s)	22, 69, 87, 97, 374
HpyCH4III	ACNGT	2 cut(s)	409, 1112
HpyCH4IV	ACGT	1 cut(s)	261
HpyF10VI	GCNNNNNNNGC	7 cut(s)	368, 503, 560, 842, 917, 1145, 1175
HpyF3I	CTNAG	4 cut(s)	615, 764, 877, 997
HpySE526I	ACGT	1 cut(s)	261
Hsp92II	CATG	7 cut(s)	166, 388, 445, 625, 662, 957, 1178
HspAI	GCGC	3 cut(s)	117, 925, 1169
Kzo9I	GATC	5 cut(s)	199, 280, 445, 570, 652
LguI	GCTCTTC	1 cut(s)	732
LmnI	GCTCC	1 cut(s)	268
Lsp1109I	GCAGC	5 cut(s)	325, 572, 895, 1048, 1135
LweI	GCATC	8 cut(s)	178, 325, 358, 541, 674, 751, 823, 929
MaeI	CTAG	2 cut(s)	507, 1154
MaeII	ACGT	1 cut(s)	261
MaeIII	GTNAC	5 cut(s)	158, 253, 299, 316, 862
MalI	GATC	5 cut(s)	201, 282, 447, 572, 654
MboI	GATC	5 cut(s)	199, 280, 445, 570, 652
MboII	GAAGA	8 cut(s)	32, 82, 304, 335, 384, 650, 749, 1174
MfeI	CAATTG	2 cut(s)	63, 309
MflI	RGATCY	1 cut(s)	445
MluCI	AATT	7 cut(s)	63, 309, 411, 521, 543, 732, 887
MlyI	GAGTC	1 cut(s)	575
MmeI	TCCRAC	1 cut(s)	228
MnlI	CCTC	8 cut(s)	122, 294, 599, 679, 793, 947, 1039, 1111
MroXI	GAANNNNTTC	1 cut(s)	32
MseI	TTAA	2 cut(s)	705, 849
MslI	CAYNNNNRTG	1 cut(s)	186
MspA1I	CMGCKG	1 cut(s)	1080
MspCI	CTTAAG	1 cut(s)	848
MspI	CCGG	2 cut(s)	131, 1088
MunI	CAATTG	2 cut(s)	63, 309
MvnI	CGCG	1 cut(s)	925
MwoI	GCNNNNNNNGC	7 cut(s)	368, 503, 560, 842, 917, 1145, 1175
NcoI	CCATGG	1 cut(s)	441
NdeII	GATC	5 cut(s)	199, 280, 445, 570, 652
NlaIII	CATG	7 cut(s)	166, 388, 445, 625, 662, 957, 1178
NlaIV	GGNNCC	2 cut(s)	135, 270
NmeAIII	GCCGAG	1 cut(s)	93
NmuCI	GTSAC	3 cut(s)	158, 299, 862
NsbI	TGCGCA	1 cut(s)	1170
NspI	RCATGY	3 cut(s)	166, 662, 1178
PaeR7I	CTCGAG	1 cut(s)	822
PciI	ACATGT	1 cut(s)	162
PciSI	GCTCTTC	1 cut(s)	732
PdmI	GAANNNNTTC	1 cut(s)	32
PfeI	GAWTC	3 cut(s)	124, 233, 472
PkrI	GCNGC	9 cut(s)	10, 340, 541, 562, 646, 910, 971, 1063, 1150
PleI	GAGTC	1 cut(s)	575
PpsI	GAGTC	1 cut(s)	575
PscI	ACATGT	1 cut(s)	162
PsiI	TTATAA	1 cut(s)	452
PspEI	GGTNACC	1 cut(s)	299
PspN4I	GGNNCC	2 cut(s)	135, 270
PspPI	GGNCC	1 cut(s)	962
PsuI	RGATCY	1 cut(s)	445
RsaI	GTAC	1 cut(s)	1010
RsaNI	GTAC	1 cut(s)	1009
RseI	CAYNNNNRTG	1 cut(s)	186
SapI	GCTCTTC	1 cut(s)	732
SaqAI	TTAA	2 cut(s)	705, 849
SatI	GCNGC	9 cut(s)	9, 339, 540, 561, 645, 909, 970, 1062, 1149
Sau3AI	GATC	5 cut(s)	199, 280, 445, 570, 652
Sau96I	GGNCC	1 cut(s)	962
SchI	GAGTC	1 cut(s)	575
SfaNI	GCATC	8 cut(s)	178, 325, 358, 541, 674, 751, 823, 929
Sfr274I	CTCGAG	1 cut(s)	822
SinI	GGWCC	1 cut(s)	962
SlaI	CTCGAG	1 cut(s)	822
SmiMI	CAYNNNNRTG	1 cut(s)	186
SmlI	CTYRAG	2 cut(s)	822, 848
SmoI	CTYRAG	2 cut(s)	822, 848
Sse9I	AATT	7 cut(s)	63, 309, 411, 521, 543, 732, 887
SsiI	CCGC	7 cut(s)	128, 539, 644, 808, 948, 970, 1080
SspI	AATATT	1 cut(s)	226
SspMI	CTAG	2 cut(s)	507, 1154
StyI	CCWWGG	1 cut(s)	441
TaaI	ACNGT	2 cut(s)	409, 1112
TaiI	ACGT	1 cut(s)	264
TaqI	TCGA	2 cut(s)	366, 823
TasI	AATT	7 cut(s)	63, 309, 411, 521, 543, 732, 887
TatI	WGTACW	1 cut(s)	1008
TauI	GCSGC	3 cut(s)	542, 647, 972
TfiI	GAWTC	3 cut(s)	124, 233, 472
Tru1I	TTAA	2 cut(s)	705, 849
Tru9I	TTAA	2 cut(s)	705, 849
TscAI	CASTG	1 cut(s)	871
TseFI	GTSAC	3 cut(s)	158, 299, 862
TseI	GCWGC	6 cut(s)	8, 338, 560, 908, 1061, 1148
Tsp45I	GTSAC	3 cut(s)	158, 299, 862
TspDTI	ATGAA	3 cut(s)	25, 771, 964
TspGWI	ACGGA	1 cut(s)	335
TspRI	CASTG	1 cut(s)	871
Vha464I	CTTAAG	1 cut(s)	848
VpaK11BI	GGWCC	1 cut(s)	962
XceI	RCATGY	3 cut(s)	166, 662, 1178
XhoI	CTCGAG	1 cut(s)	822
XmiI	GTMKAC	2 cut(s)	871, 989
XmnI	GAANNNNTTC	1 cut(s)	32
XspI	CTAG	2 cut(s)	507, 1154

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.