Rh7AG012900

TPR repeat-containing thioredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0006594
Physical Location & Seq
Forward (+)
866987 .. 871669
4683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG012900.1

Sequence Viewer

Length: 954 bp
ATGGAAACTAAAGAACAGTTTGATGATGACATTGTTGAATCTGATATTGAGTTGGATGATGCTGATGTTGTGGAGCCTGATAATGATCCTCCGCAACAGATGGGAGACCCATCAGTTGAAGTTACAGAAGAAGTGCAGGATGCTGCGCAGTTGGCGAAATCGAGAGCTATGGATGCTGTTTCTGAAGGTAAACTGGATGAGGCCATAGATCATATAACAGAAGCCATCATGTTGAATCCAACTTCAGCCATACTAAAAGCATCCAGAGCTAGTATCTTTGTCAAATTGAACAAACCAAATGCGGCAATTCGTGATGCTAATGCAGCTATAGAGATCAACCCGGACTCAGCAAAAGGATATAAAATAAGAGGAACTGCAAAGGCTATGTTGGGCCACTGGGAAGAAGCAGCAAGTGACCTGCATATAGCATCCAAACTAGATTATGATGAGGAGATTGGTTTGGTGCTTAAAAAGGTTGAACCTAATGCTCGAAAAATTGAAGAGCATCGCAAAAAATATCAACGGTTGCATAAAGAAAGGGAGATGAAAAGGGCTGAACATGAGAGACAGAGGCAAGCTGAAGCTCGAGAACGGGATGCTTTGTCTGCTCTTAAGGATGGGCAAGTTATTGGTATAAATGGGGCCAGTGAACTTGAGACGAAATTGAATGCTGCTTCAAGGACATCGCGCCTTGCAATCCTGTACTTCACCGCGACATGGTGTGGTCCTTGCCGTACAATATCTCCACTATATACAAGCTTAGCTGAAAAGTACCCAAAAGTAGTTTTCACGAAAATTGACATAGATGTGGCAAGAGCTGTGGCTGAAAGCTGGAATATCAGCAGTGTTCCAGCCTTTTTCTTTGTAAGAAATGGCAAGGAGATTGACAAGGTTGTGGGTGCTGATAAAGCTGCGCTAGAAAAGAAGATTGCACAATATGCAGCCTCAGCATAA

Protein Analysis

317

Amino Acids

35.12

Weight (kDa)

5.23

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 228 - 311 9.6e-23 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 147
Acc36I ACCTGC 1 cut(s) 426
AccII CGCG 2 cut(s) 688, 713
AciI CCGC 3 cut(s) 92, 302, 711
AclWI GGATC 1 cut(s) 80
AcuI CTGAAG 3 cut(s) 204, 228, 600
AfaI GTAC 3 cut(s) 704, 736, 773
AfiI CCNNNNNNNGG 1 cut(s) 717
AflII CTTAAG 1 cut(s) 611
AgsI TTSAA 8 cut(s) 38, 119, 235, 289, 479, 500, 667, 678
Alw26I GTCTC 3 cut(s) 99, 559, 650
AlwI GGATC 1 cut(s) 80
Ama87I CYCGRG 1 cut(s) 585
AoxI GGCC 3 cut(s) 201, 391, 642
ApeKI GCWGC 6 cut(s) 143, 323, 407, 671, 911, 941
AspLEI GCGC 3 cut(s) 148, 690, 916
AspS9I GGNCC 3 cut(s) 391, 642, 725
AsuC2I CCSGG 1 cut(s) 341
AsuHPI GGTGA 1 cut(s) 700
AvaI CYCGRG 1 cut(s) 585
AvaII GGWCC 1 cut(s) 725
BbvCI CCTCAGC 1 cut(s) 946
BbvI GCAGC 5 cut(s) 130, 335, 419, 658, 898
BccI CCATC 4 cut(s) 94, 118, 233, 611
BceAI ACGGC 1 cut(s) 717
BcnI CCSGG 1 cut(s) 341
BcoDI GTCTC 3 cut(s) 99, 559, 650
BfaI CTAG 3 cut(s) 270, 437, 917
BfmI CTRYAG 1 cut(s) 327
BfrI CTTAAG 1 cut(s) 611
BfuAI ACCTGC 1 cut(s) 426
BisI GCNGC 7 cut(s) 144, 303, 324, 408, 672, 912, 942
BlpI GCTNAGC 1 cut(s) 760
BlsI GCNGC 7 cut(s) 145, 304, 325, 409, 673, 913, 943
Bme1390I CCNGG 1 cut(s) 341
Bme18I GGWCC 1 cut(s) 725
BmeT110I CYCGRG 1 cut(s) 585
BmgT120I GGNCC 3 cut(s) 391, 642, 725
BmiI GGNNCC 2 cut(s) 75, 643
BmrFI CCNGG 1 cut(s) 341
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 8 cut(s) 49, 130, 163, 269, 304, 437, 514, 586
BmuI ACTGGG 1 cut(s) 406
Bpu10I CCTNAGC 1 cut(s) 946
Bpu1102I GCTNAGC 1 cut(s) 760
BpuEI CTTGAG 1 cut(s) 674
BpuMI CCSGG 1 cut(s) 341
BsaBI GATNNNNATC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 99
BsaXI ACNNNNNCTCC 2 cut(s) 727, 757
Bsc4I CCNNNNNNNGG 1 cut(s) 717
Bse1I ACTGG 3 cut(s) 198, 401, 645
Bse8I GATNNNNATC 1 cut(s) 84
BseGI GGATG 8 cut(s) 61, 145, 178, 202, 260, 428, 601, 622
BseJI GATNNNNATC 1 cut(s) 84
BseLI CCNNNNNNNGG 1 cut(s) 717
BseMII CTCAG 1 cut(s) 360
BseNI ACTGG 3 cut(s) 198, 401, 645
BseRI GAGGAG 1 cut(s) 464
BseXI GCAGC 5 cut(s) 130, 335, 419, 658, 898
BsgI GTGCAG 1 cut(s) 155
Bsh1236I CGCG 2 cut(s) 688, 713
BshFI GGCC 3 cut(s) 203, 393, 644
BsiHKCI CYCGRG 1 cut(s) 585
BsiSI CCGG 1 cut(s) 341
BslI CCNNNNNNNGG 1 cut(s) 717
BsmAI GTCTC 3 cut(s) 99, 559, 650
BsmBI CGTCTC 1 cut(s) 650
BsmI GAATGC 1 cut(s) 673
BsnI GGCC 3 cut(s) 203, 393, 644
Bso31I GGTCTC 1 cut(s) 99
BsoBI CYCGRG 1 cut(s) 585
Bsp143I GATC 3 cut(s) 85, 208, 333
Bsp1720I GCTNAGC 1 cut(s) 760
BspACI CCGC 3 cut(s) 92, 302, 711
BspANI GGCC 3 cut(s) 203, 393, 644
BspCNI CTCAG 1 cut(s) 359
BspFNI CGCG 2 cut(s) 688, 713
BspLI GGNNCC 2 cut(s) 75, 643
BspMI ACCTGC 1 cut(s) 426
BspPI GGATC 1 cut(s) 80
BspQI GCTCTTC 1 cut(s) 495
BspTI CTTAAG 1 cut(s) 611
BspTNI GGTCTC 1 cut(s) 99
BsrI ACTGG 3 cut(s) 198, 401, 645
BssMI GATC 3 cut(s) 85, 208, 333
Bst4CI ACNGT 2 cut(s) 18, 525
Bst6I CTCTTC 1 cut(s) 495
BstAFI CTTAAG 1 cut(s) 611
BstAPI GCANNNNNTGC 1 cut(s) 938
BstC8I GCNNGC 1 cut(s) 576
BstDEI CTNAG 3 cut(s) 346, 760, 946
BstF5I GGATG 8 cut(s) 61, 145, 178, 202, 260, 428, 601, 622
BstFNI CGCG 2 cut(s) 688, 713
BstHHI GCGC 3 cut(s) 148, 690, 916
BstKTI GATC 3 cut(s) 88, 211, 336
BstMAI GTCTC 3 cut(s) 99, 559, 650
BstMBI GATC 3 cut(s) 85, 208, 333
BstMWI GCNNNNNNNGC 8 cut(s) 152, 173, 266, 323, 605, 908, 938, 947
BstSCI CCNGG 1 cut(s) 339
BstSFI CTRYAG 1 cut(s) 327
BstUI CGCG 2 cut(s) 688, 713
BstV1I GCAGC 5 cut(s) 130, 335, 419, 658, 898
BsuRI GGCC 3 cut(s) 203, 393, 644
BtgZI GCGATG 2 cut(s) 491, 669
BtsCI GGATG 8 cut(s) 61, 145, 178, 202, 260, 428, 601, 622
BtsI GCAGTG 1 cut(s) 850
BtsIMutI CAGTG 3 cut(s) 394, 652, 850
BveI ACCTGC 1 cut(s) 426
Cac8I GCNNGC 1 cut(s) 576
CfoI GCGC 3 cut(s) 148, 690, 916
Cfr13I GGNCC 3 cut(s) 391, 642, 725
Csp6I GTAC 3 cut(s) 703, 735, 772
CspCI CAANNNNNGTGG 2 cut(s) 801, 836
CviAII CATG 3 cut(s) 229, 560, 717
CviQI GTAC 3 cut(s) 703, 735, 772
DdeI CTNAG 3 cut(s) 346, 760, 946
DpnI GATC 3 cut(s) 87, 210, 335
DpnII GATC 3 cut(s) 85, 208, 333
Eam1104I CTCTTC 1 cut(s) 495
EarI CTCTTC 1 cut(s) 495
Eco31I GGTCTC 1 cut(s) 99
Eco47I GGWCC 1 cut(s) 725
Eco57I CTGAAG 3 cut(s) 204, 228, 600
Eco88I CYCGRG 1 cut(s) 585
Esp3I CGTCTC 1 cut(s) 650
FaeI CATG 3 cut(s) 232, 563, 720
FatI CATG 3 cut(s) 228, 559, 716
Fnu4HI GCNGC 7 cut(s) 144, 303, 324, 408, 672, 912, 942
FokI GGATG 8 cut(s) 68, 152, 185, 209, 247, 415, 608, 629
Fsp4HI GCNGC 7 cut(s) 144, 303, 324, 408, 672, 912, 942
FspBI CTAG 3 cut(s) 270, 437, 917
FspI TGCGCA 1 cut(s) 147
GlaI GCGC 3 cut(s) 147, 689, 915
GluI GCNGC 7 cut(s) 144, 303, 324, 408, 672, 912, 942
HaeIII GGCC 3 cut(s) 203, 393, 644
HapII CCGG 1 cut(s) 341
HhaI GCGC 3 cut(s) 148, 690, 916
Hin1II CATG 3 cut(s) 232, 563, 720
Hin6I GCGC 3 cut(s) 146, 688, 914
HinP1I GCGC 3 cut(s) 146, 688, 914
HindIII AAGCTT 1 cut(s) 757
HinfI GANTC 3 cut(s) 38, 235, 344
HpaII CCGG 1 cut(s) 341
HphI GGTGA 1 cut(s) 700
Hpy166II GTNNAC 2 cut(s) 191, 650
Hpy188I TCNGA 2 cut(s) 43, 184
Hpy188III TCNNGA 5 cut(s) 162, 264, 311, 587, 790
Hpy8I GTNNAC 2 cut(s) 191, 650
HpyAV CCTTC 1 cut(s) 179
HpyCH4III ACNGT 2 cut(s) 18, 525
HpyCH4V TGCA 8 cut(s) 136, 323, 377, 421, 529, 695, 932, 941
HpyF10VI GCNNNNNNNGC 8 cut(s) 152, 173, 266, 323, 605, 908, 938, 947
HpyF3I CTNAG 3 cut(s) 346, 760, 946
Hsp92II CATG 3 cut(s) 232, 563, 720
HspAI GCGC 3 cut(s) 146, 688, 914
Kzo9I GATC 3 cut(s) 85, 208, 333
LguI GCTCTTC 1 cut(s) 495
LmnI GCTCC 1 cut(s) 73
Lsp1109I GCAGC 5 cut(s) 130, 335, 419, 658, 898
LweI GCATC 8 cut(s) 49, 130, 163, 269, 304, 437, 514, 586
MaeI CTAG 3 cut(s) 270, 437, 917
MaeIII GTNAC 2 cut(s) 121, 413
MalI GATC 3 cut(s) 87, 210, 335
MboI GATC 3 cut(s) 85, 208, 333
MboII GAAGA 4 cut(s) 140, 413, 512, 937
MluCI AATT 5 cut(s) 284, 306, 495, 662, 795
MlyI GAGTC 1 cut(s) 338
MmeI TCCRAC 2 cut(s) 33, 263
MnlI CCTC 5 cut(s) 99, 193, 362, 442, 564
MseI TTAA 2 cut(s) 468, 612
MslI CAYNNNNRTG 1 cut(s) 806
MspCI CTTAAG 1 cut(s) 611
MspI CCGG 1 cut(s) 341
MspR9I CCNGG 1 cut(s) 341
Mva1269I GAATGC 1 cut(s) 673
MvnI CGCG 2 cut(s) 688, 713
MwoI GCNNNNNNNGC 8 cut(s) 152, 173, 266, 323, 605, 908, 938, 947
NciI CCSGG 1 cut(s) 341
NdeII GATC 3 cut(s) 85, 208, 333
NlaIII CATG 3 cut(s) 232, 563, 720
NlaIV GGNNCC 2 cut(s) 75, 643
NmuCI GTSAC 1 cut(s) 413
NsbI TGCGCA 1 cut(s) 147
PaeR7I CTCGAG 1 cut(s) 585
PciSI GCTCTTC 1 cut(s) 495
PctI GAATGC 1 cut(s) 673
PfeI GAWTC 2 cut(s) 38, 235
PkrI GCNGC 7 cut(s) 145, 304, 325, 409, 673, 913, 943
PleI GAGTC 1 cut(s) 338
PpsI GAGTC 1 cut(s) 338
PspN4I GGNNCC 2 cut(s) 75, 643
PspPI GGNCC 3 cut(s) 391, 642, 725
RsaI GTAC 3 cut(s) 704, 736, 773
RsaNI GTAC 3 cut(s) 703, 735, 772
RseI CAYNNNNRTG 1 cut(s) 806
SapI GCTCTTC 1 cut(s) 495
SaqAI TTAA 2 cut(s) 468, 612
SatI GCNGC 7 cut(s) 144, 303, 324, 408, 672, 912, 942
Sau3AI GATC 3 cut(s) 85, 208, 333
Sau96I GGNCC 3 cut(s) 391, 642, 725
SchI GAGTC 1 cut(s) 338
ScrFI CCNGG 1 cut(s) 341
SfaNI GCATC 8 cut(s) 49, 130, 163, 269, 304, 437, 514, 586
SfcI CTRYAG 1 cut(s) 327
Sfr274I CTCGAG 1 cut(s) 585
SinI GGWCC 1 cut(s) 725
SlaI CTCGAG 1 cut(s) 585
SmiMI CAYNNNNRTG 1 cut(s) 806
SmlI CTYRAG 3 cut(s) 585, 611, 653
SmoI CTYRAG 3 cut(s) 585, 611, 653
Sse9I AATT 5 cut(s) 284, 306, 495, 662, 795
SsiI CCGC 3 cut(s) 92, 302, 711
SspMI CTAG 3 cut(s) 270, 437, 917
StyD4I CCNGG 1 cut(s) 339
TaaI ACNGT 2 cut(s) 18, 525
TaqI TCGA 3 cut(s) 161, 490, 586
TasI AATT 5 cut(s) 284, 306, 495, 662, 795
TatI WGTACW 1 cut(s) 702
TauI GCSGC 1 cut(s) 305
TfiI GAWTC 2 cut(s) 38, 235
Tru1I TTAA 2 cut(s) 468, 612
Tru9I TTAA 2 cut(s) 468, 612
TscAI CASTG 3 cut(s) 401, 652, 850
TseFI GTSAC 1 cut(s) 413
TseI GCWGC 6 cut(s) 143, 323, 407, 671, 911, 941
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 1 cut(s) 560
TspRI CASTG 3 cut(s) 401, 652, 850
Vha464I CTTAAG 1 cut(s) 611
VpaK11BI GGWCC 1 cut(s) 725
XhoI CTCGAG 1 cut(s) 585
XspI CTAG 3 cut(s) 270, 437, 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.